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Method used for extracting high purity vincristine from cultured catharanthus roseus

A technology for vincristine and vinblastine, applied in the field of extracting high-purity vincristine, can solve the problems of waste, low extraction rate, low vinblastine content and the like

Inactive Publication Date: 2016-01-20
CHANGZHOU OPTICAL MATERIAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem mainly solved by the present invention: aiming at the low content of vinblastine in periwinkle in my country at present, the extraction purity generally does not reach 98%, and the extraction rate is lower than 30%, which reduces the value of its industrial production and causes a lot of waste, The invention provides a method of cultivating periwinkle by mixing activated sludge, corn stalks, phosphate enol-pyruvate, etc. as a culture substrate, and then spraying the mixed solution to increase the content of vinblastine in periwinkle, and then After its treatment, it is soaked in salt water, fermented, concentrated and purified to obtain vinblastine

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0020] Take 5kg of activated sludge, 2.3kg of 40-mesh corn stalk powder, 1kg of glucose, 0.5kg of enol-pyruvate phosphate, 1kg of tryptophan with a concentration of 80mg / L and 0.2kg of methyl jasmonate in a container, and stir evenly; Put the mixture obtained above into the fermentation tank, set the temperature at 30°C, seal and stir the fermentation for 5 hours to remove the fermentation liquid, use the fermented material as the culture substrate, spread it evenly in the culture tank with a thickness of 8cm, and plant 2 pairs of true rice per cubic meter. The periwinkle leaves are moved into the culture tank for cultivation, the temperature is maintained at 20°C, the water content of the culture medium is 20%, and the mixed solution is sprayed every other day. The mixed solution is 8% hydrochloric acid according to the mass fraction per liter. Add 84g6-furfuryl aminopurine to the solution and mix it evenly. The amount of spraying is to spray 4mL of the mixed solution on each ...

example 2

[0023]Get 5.5kg of activated sludge, 2.3kg of 60 mesh corn stalk powder, 0.8kg of glucose, 0.5kg of enol-pyruvate phosphate, 0.8kg of 100mg / L tryptophan and 0.1kg of methyl jasmonate into the container. Stir evenly; put the above-mentioned mixture into the fermentation tank, set the temperature at 35°C, seal and stir for 7 hours to remove the fermentation liquid, use the fermented product as a culture medium, spread it evenly in the culture tank with a thickness of 10cm, and calculate it per cubic meter Plant 4 pairs of true leaves of periwinkle, move into the culture tank for cultivation, keep the temperature at 33°C, and the water content of the culture medium is 35%, and spray the mixed solution every 2 days, and the mixed solution is according to the mass fraction per liter of 8% hydrochloric acid solution is added into 84g6-furfuryl aminopurine and uniformly mixed, and the spraying amount is to spray 5mL of the mixed solution on each periwinkle, and when the periwinkle bea...

example 3

[0026] Take 5.2kg of activated sludge, 2.3kg of 40-60 mesh corn stalk powder, 0.9kg of glucose, 0.5kg of phosphate enol-pyruvate, 0.9kg of 90mg / L tryptophan and 0.2kg of methyl jasmonate into the container and stir evenly; put the mixture obtained above into a fermentation tank, set the temperature at 32°C, seal and stir for 6 hours to remove the fermentation liquid, use the fermented product as a culture medium, spread it evenly in the culture tank with a thickness of 9cm, and press Plant 3 pairs of true leaves of periwinkle per cubic meter, move it into the culture pool and cultivate it, keep the temperature at 25°C, and the water content of the culture substrate is 25%, spray the mixed solution every other day, and the mixed solution is based on the mass per liter The hydrochloric acid solution that fraction is 8% is added 84g6-furfuryl aminopurine and is evenly mixed to form, and its spraying amount is that every periwinkle sprays 5mL mixed solution, when periwinkle bears f...

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Abstract

The invention discloses a method used for extracting high purity vincristine from cultured catharanthus roseus, and belongs to the technical field of biotechnology. The method is used for solving problems of the prior art that, catharanthus roseus is low in vinblastine content, extraction purity is lower than 98%, extraction rate is lower than 30%, industrialized production value is reduced, and huge waste is caused. According to the method, active sludge, corn stalk, and phosphoenolpyruvic acid are mixed so as to obtain a culture medium used for culturing catharanthus roseus; a mixed solution is sprayed so as to increase vincristine content of catharanthus roseus; the cultured catharanthus roseus is subjected to treatment, salt solution immersion, fermentation, and concentration so as to purify vinblastine. The method is unique; it is confirmed by detection that vinblastine content of the catharanthus roseus obtained culturing is increased by 20 to 30%, the purity of obtained vinblastine is higher than 98%, and extraction rate ranges from 55 to 60%.

Description

technical field [0001] The invention relates to a method for extracting high-purity vincristine from cultivated periwinkle, belonging to the field of biotechnology. Background technique [0002] Vinblastine, also known as vinblastine, and its sulfate, vinblastine sulfate, are national essential medicines, mainly used for the treatment of Hodgkin's disease and choriocarcinoma. At present, vinblastine flower alkaloids are generally extracted from vinca, a plant of the family Apocynaceae. Vinca contains nearly a hundred kinds of alkaloids, most of which are active, among them, vinblastine sulfate, but because the content of vinblastine in vinca is extremely low, it is difficult to realize industrial production by chemical synthesis methods, therefore, it is difficult to find high-content vinblastine Raw materials for the production of vinca alkaloids are crucial. [0003] So far, my country has only reported the patent on the extraction of vinblastine, but there is no special...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D519/04A01G31/00
CPCC07D519/04A01G31/00
Inventor 陈毅忠宋国
Owner CHANGZHOU OPTICAL MATERIAL
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