34 results about "Candidatus Liberibacter" patented technology

A Candidatus Liberibacter spp. online rapid detection system based on deep learning includes a mobile phone client and a server end; the server end includes a data transmission module, a leaf and fruit detection module, a Candidatus Liberibacter spp. diagnosis module, and a sample database; a model of the leaf and fruit detection module is trained by using the data with instance annotations in a sample database to obtain the optimal model; a model of the Candidatus Liberibacter spp. diagnosis module is trained by using the data with diagnostic annotations in the sample database to obtain the optimal model; the leaf and fruit detection module transmits the output and detected single leaf and single fruit images to the Candidatus Liberibacter spp. diagnosis module. A Candidatus Liberibacterspp. online rapid detection method based on deep learning adopts the Candidatus Liberibacter spp. online rapid detection system based on deep learning. The invention belongs to the technical field ofintelligent identification of Candidatus Liberibacter spp., and has the advantages of rapid, efficient and reliable detection.

The invention relates to a deep learning-based Candidatus Liberibacter spp detection method, device and system. The method comprises the steps: acquiring image data of citrus leaves to be identified;Inputting the image data into a neural network model of Candidatus Liberibacter spp detection of a mobile terminal; And determining a detection result corresponding to the image data. The detection method is simple and convenient to operate, lossless, low in cost and popular with citrus producers; Moreover, the diagnosis process is simplified, the diagnosis cost is reduced, fruit farmers can be helped to detect and discover diseases as soon as possible, rapid, real-time, accurate and non-destructive diagnosis on citrus fruit trees in orchards is realized, reference can be provided for fertilization and production of the fruit farmers, great help is provided for the fruit tree yield, and a positive role is played in promoting accurate agriculture and agricultural informatization.

The present invention relates to the use of compositions that, when applied to citrus, are capable of controlling the psyllids Trioza erytreae and Diaphorina citri, vectors of bacteria of the genus Candidatus liberibacter, which cause the disease known as Huanglongbing (HLB), deemed to be the most destructive disease currently to affect citrus crops worldwide. These compositions may contain, without distinction, or jointly, as active components, vitamin K3, soluble derivatives thereof (preferably menadione sodium bisulphite [MSB]) or sparingly soluble derivatives thereof (preferably menadione nicotinamide bisulphite [MNB]), with respective concentrations of between 0.0001 and 200 ppm, 0.001 and 10 000 ppm, preferably vitamin K3 between 0.001 and 100 ppm, MSB between 0.01 and 5000 ppm, and MNB between 0.01 and 5000 ppm. Said compositions are applied preferably by spraying to the aerial part of the plant or via the irrigation system, and may be mixed with a variety of additives, such as organic and inorganic fertilizers, insecticides, nematocides, fungicides, bactericides, acaricides or herbicides.

The invention discloses a method for identifying Candidatus Liberibacter spp. of different varieties of citrus by adopting model transfer. According to the method, an LS-SVM discrimination model for citrus Candidatus Liberibacter spp. in different seasons is established based on a hyperspectral image technology, and then spectral data of different varieties of citrus are corrected by adopting a piecewise direct correction algorithm (PDS) in combination with a model updating method, so that the citrus Candidatus Liberibacter spp. in different varieties and different seasons is identified.

The invention discloses an application of a Candidatus Liberibacter asiaticum effector as a target for screening anti-Candidatus Liberibacter asiaticum drugs, and finds that the Candidatus Liberibacter asiaticum flagellum matrix P-ring protein as the effector can cause significant callose deposition, and an inhibitor of the P-ring protein can be used for preventing and treating Candidatus Liberibacter asiaticum, and has a good application prospect in the field of prevention and treatment of Candidatus Liberobacter asiaticum. Meanwhile, the characteristics of small genome, simple culture, short life cycle and stable genetic manipulation of the saccharomyces cerevisiae are utilized, and the saccharomyces cerevisiae is used as a model organism for researching the effector of the bacterium CLas. Candidatus liberobacter asiaticum effector bacterium flagellum matrix P-ring protein is overexpressed in the saccharomyces cerevisiae, so that the inherent physiological function of the saccharomyces cerevisiae is disturbed, the phenotype of the saccharomyces cerevisiae is changed, and the proliferation of saccharomyces cerevisiae cells is inhibited. Meanwhile, transient expression in the Bensi tobacco leaves under agrobacterium tumefaciens mediation verifies and identifies that the gene can induce plant defense reactions such as local allergic necrosis of tobacco leaves and callose deposition.

The invention provides a bactericide compound for preventing and treating candidatus liberibacter and a preparation method thereof. The bactericide compound is a compound composition prepared from antibiotics, ammonium nitrate, monopotassium phosphate, magnesium sulfate, EDDHA-Fr, EDTA-Mn, EDTA-Zn, EDTA-Cu, EDTA-Co, EDTA-Ca, borax, sodium molybdate, trehalose, potassium fulvic acid, a stabilizing agent, an antifreezing agent, novel permeation promoter, a dispersing agent, an emulsifying agent and co-solvent, the pH value is regulated to 6.5, and 1000-2000ml of water is added. The composition is reasonably mixed by bactericide and multiple nutritional substances according to objective requirement of prevention and control of candidatus liberibacter, is applied by adopting a trunk transfusion method to complement advantages, has the characteristics of high efficiency, low toxicity, safety and multiple functions, and can be used for better preventing and controlling candidatus liberibacter.

The invention discloses a citrus candidatus liberibacter and citrus canker pathogen dual detection kit and application thereof. A dual digital PCR quantitative detection agent composition for the citrus candidatus liberibacter and citrus canker pathogen is designed. The kit comprises a citrus disease sample DNA extraction agent, a digital PCR reaction agent, a citrus liberibacter asiaticus and citrus canker positive control sample template and a citrus liberibacter asiaticus and citrus canker negative control sample template. The kit has the advantages of being high in specificity, higher in impurity interference resistance, simple and fast when used for detecting the citrus candidatus liberibacter and citrus canker pathogen, the detection sensitivity is higher than that of an existing real-time fluorescence quantification PCR detection method by about 10 times, and the kit can be used for early-stage qualitative and quantitative detection of the citrus candidatus liberibacter and citrus canker pathogen and epidemiologic investigation.

A method and compounds are described and claimed relating to plant defense inducer compositions and compounds, and to their use for improving certain aspects of plant and crop management, including treating plant disease, improving the ability of plants to defend against disease, reducing disease symptoms, treating HLB disease, minimizing crop yield decreases due to plant disease, improving crop productivity, and increasing crop quality. In particular, the compounds and compositions are useful for controlling and treating plant disease, for example Huanglongbing disease (HLB) and mitigating disease symptoms of HLB and Ca. Liberibacter infection in plants. Crop plants are contemplated for use with the invention, for example citrus trees. Methods according to embodiments of the invention involve treatment of affected plants by application (preferably by injection into the trunk, soil application or foliar spraying) of one or more compounds selected from β-aminobutyric acid or a salt thereof, 2-deoxy-D-glucose, salicylic acid or a salt thereof, oxalic acid. Typical methods involve application of a composition containing a botanically acceptable vehicle and β-aminobutyric acid. In certain methods, the plants used in the method are citrus plants, and the citrus plants are affected by or susceptible to Huanglongbing (HLB) disease or infection by Candidatus Liberibacter species.

The invention discloses a method for analyzing genetic diversity of a Candidatus Liberibacter by utilizing an SSR (simple sequence repeat) molecular marker primer system. The SSR molecular marker primer system comprises 33 pairs of Las-SSR available primers, 18 pairs of which are SSR core primers. The invention puts forward the SSR molecular marker based method for analyzing diversity of a Ca.L.asiaticus strain in combination with PAGE (polyacrylamide gelelectrophoresis). The method is simple and convenient, is high in analysis efficiency, and lays a molecular foundation for study of Candidatus Liberibacter molecular epidemiology.

The invention relates to a method for screening a Candidatus Liberibacter resisting chemical through catharanthus roseus. The method includes the steps of cultivation of diseased catharanthus roseus plants, cutting regeneration of catharanthus roseus, optimization of cutting regeneration conditions of catharanthus roseus, chemical treatment and screening of the Candidatus Liberibacter resisting chemical. According to the method, the natural host catharanthus roseus for the plant Candidatus Liberibacter is taken as a test material for screening the Candidatus Liberibacter resisting chemical, the screening method is fast, easy and convenient to operate, and the effect of the chemical to be screened on resistance to Candidatus Liberibacter can be evaluated visually and accurately.

The present invention relates to the use of dithiine-tetracarboximides of formula (I) for controlling selected bacterial harmful organisms in useful plants, wherein the bacterial harmful organisms are selected from the group consisting of Acidovorax avenae, Burkholderia spec., Burkholderia glumae, Candidatus Liberibacter spec., Candidatus Liberibacter asiaticus, Corynebacterium, Erwinia spec. (Dickeya, Pectobacterium carotovorum, Erwinia amylovora), Pseudomonas syringae, Pseudomonas syringae pv. actinidae, Pseudomonas syringae pv. glycinea, Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. lachrymans, Pseudomonas tumefaciens (=Agrobacterium tumefaciens), Streptomyces spp., Xanthomonas spp., Xanthomonas ampelina, Xanthomonas axonopodis, Xanthomonas axonopodis pv. citri, Xanthomonas axonopodis pv. glycines, Xanthomonas campestris, Xanthomonas campestris pv. musacearum, Xanthomonas campestris pv. pruni, Xanthomonas campestris pv. Viticola, Xanthomonas fragariae and Xanthomonas transluscens or Xylella fastidiosa. The present invention also relates to a method for controlling the selected bacterial harmful organisms in useful plants by treatment with a dithiine-tetracarboximides of formula (I).

The invention relates to the technical field of fertilizer, in particular to water soluble fertilizer for controlling candidates liberibacter of fruit trees. The water soluble fertilizer is prepared from the following raw materials in parts by weight: 20 to 50 parts of composite microbial fungicide, 20 to 300 parts of organic matters, 90 to 120 parts of medium-trace element soil conditioning agent, 300 to 450 parts of water, 75 to 85 parts of water-soluble coal-based mineral potassium humate or water-soluble coal-based mineral sodium humate, 10 to 125 parts of dipotassium hydrogen phosphate, 20 to 300 parts of urea, 50 to 500 parts of potassium tripolyphosphate, 50 to 500 parts of potassium formate, 5 to 15 parts of borax, boric acid or disodium octoborate tetrahydrate, 10 to 50 parts of potassium hydroxide, 10 to 25 parts of organic acid, 5 to 20 parts of EDTA complex trace elements, 3 to 5 parts of AES or AEO, and 0.5 to 1.2 parts of xanthan gum. By adopting the water soluble fertilizer for controlling the candidates liberibacter of fruit trees, the low-solubility salts and precipitation effect can be avoided, a good sustained release effect for the fertilizer can be achieved, the buffering performance of the soil can be apparently improved, the PH value of the acidic soil can be increased, the acidity-alkalinity of the soil can be adjusted, the stability is high, the flocculation resistance can be apparently improved, the orchard environment can be optimized, and a purpose of high quality, high yield and high performance can be achieved.

DNA amplification methods using novel primers obtained from the novel genes for hyvI and hyvII s from the Candidatus Liberibacter asiaticus genome and useful for detecting Ca. L. species in plants and insect hosts.

Numerous plant microbes, including the vascular-limited Candidatus spp.—causal agents of citrus greening and potato zebra chip diseases—are non-culturable. The present disclosure relates, according to some embodiments, to compositions, methods and systems for culturing such organisms. For example, the present disclosure relates to methods for culturing, propagating, and characterizing fastidious vascular-colonizing microbes using a hairy root system (e.g., in vitro, in planta). The present disclosure relates, in some embodiments, to methods for cultivating a fastidious plant microbe including: contacting a plant (e.g., a tomato plant, a potato plant, a citrus plant) colonized by a fastidious plant microbe (e.g., Xylella fastidiosa, Candidatus Liberibacter spp.) with a suspension of R. rhizogenes under conditions that permit induction of hairy roots colonized with the fastidious plant microbe, and propagating the colonized microbial hairy roots.

The invention belongs to the technical field of plant disease prevention and treatment and particularly relates to a method for reducing citrus Huanglongbing pathogenic bacterium CLas (Candidatus Liberibacter asisticus) content. Carbon quantum dot nanoparticles are applied to preparation of carbon quantum dot nano oxytetracycline which is then injected to citrus plants with citrus Huanglongbing bymeans of trunk injection. Preparation of carbon quantum dot nano oxytetracycline includes carbon quantum dot nanoparticle preparation and carbon quantum dot nano oxytetracycline preparation. The carbon quantum dot nano oxytetracycline has the advantages of small size and is smaller than the pore diameter of a plant sieve tube in which pathogenic bacteria are parasitized, so that great bactericidal effects can be achieved; in addition, corresponding solution is stable and uniform in property and meets injection requirements. The method for reducing citrus Huanglongbing pathogenic bacterium CLas content is significant to citrus Huanglongbing prevention and treatment and has a potential of large-scale agricultural application.

Numerous plant microbes, including the vascular-limited Candidatus spp.—causal agents of citrus greening and potato zebra chip diseases—are non-culturable. The present disclosure relates, according to some embodiments, to compositions, methods and systems for culturing such organisms. For example, the present disclosure relates to methods for culturing, propagating, and characterizing fastidious vascular-colonizing microbes using a hairy root system (e.g., in vitro, in planta). The present disclosure relates, in some embodiments, to methods for cultivating a fastidious plant microbe including: contacting a plant (e.g., a tomato plant, a potato plant, a citrus plant) colonized by a fastidious plant microbe (e.g., Xylella fastidiosa, Candidatus Liberibacter spp.) with a suspension of R. rhizogenes under conditions that permit induction of hairy roots colonized with the fastidious plant microbe, and propagating the colonized microbial hairy roots.

The invention discloses a method for detoxification of citrus by utilizing microwaves, and belongs to the technical field of microwave application. The method is characterized in that during microwave pretreatment, the microwave irradiation frequency is 1.0 GHz-3.0 GHz, the power is 100-900 W, the microwave irradiation time is 5-20 seconds, then pausing is carried out for 10-30 seconds, repetitive operation is carried out 3-10 times, the temperature of each part of the citrus is increased to 40-45 DEG C, and the constant temperature is maintained for 30-60 seconds; then it is adjusted that the microwave irradiation frequency is 350 MHz-950 MHz, the power is 1,000-3,000 W, the microwave irradiation time is 3-10 seconds, then pausing is carried out for 20-60 seconds, repetitive operation is carried out 5-20 times, the temperature of each part of the citrus is increased to 50-55 DEG C, the constant temperature is maintained for 1-5 minutes, and citrus detoxification is carried out by utilizing the microwaves. The method for detoxification of the citrus by utilizing the microwaves can make each part of the citrus reach the fatal temperature of candidatus liberibacter more quickly and evenly, and a tree body is not affected; under the non-thermal effect of a microwave electromagnetic field, nucleic acid, protein and other molecules in the candidatus liberibacter are easy to denature and inactivate to achieve double sterilization effects; the detoxificated citrus through the microwaves are detected by adopting GB / T 28062-2011 <Real-Time Fluorescence PCR Detection Method of Candidatus Liberibacter>, and the negative conversion ratio of the candidatus liberibacter is greater than or equal to 82%. The method is an efficient, quick and environment-friendly citrus detoxification method.

The invention provides a method for obtaining CLas infected diaphorina citri polypide, and relates to the technical field of insect physiology and biochemistry. The method comprises the following steps: firstly, screening out a concentration which is most suitable for survival of in-vitro citrus twigs from Hoagland nutrient solutions with different concentrations; selecting citrus twigs infected with Candidatus Liberibacter asiaticum by using conventional PCR (Polymerase Chain Reaction) and real-time fluorescent quantitative PCR technologies, and performing in-vitro culture; the method comprises the following steps: selecting diaphorina citri adults, nymphs of 4-5 years old and nymphs of 3 years old or below, screening an optimal bacterium obtaining period, and finally obtaining diaphorina citri polypides infected with the Candidatus Liberibacter asiaticum by utilizing a constructed system, so as to provide a basic material for subsequent theoretical research.

The invention discloses a granular fertilizer and a method for delaying decline of a tree body with Huanglongbing. The combined application amount of manganese in the raw materials of the granular fertilizer is increased by about 300%, the amount of NPK is reduced by 30-50%, and the usage amount of the granular fertilizer is 1-5 kg / plant. According to the invention, it is found that excessive application of Mn can inhibit the number of Candidatus Liberibacter asiaticum pathogens in citrus root systems, the inhibition effect is better if NPK is reduced by 30-50%, and the special fertilizer designed based on the above discovery can well achieve the effect of treating root causes, and delays decline of Candidatus Liberibacter asiaticum trees.

The invention relates to the technical field of biological control, in particular to a method for screening biocontrol bacteria of Candidatus Liberibacter spp. by a two-step method. According to the method disclosed by the invention, five indicator pathogenic bacteria are used for performing confrontation culture, potential biocontrol bacteria are screened, a half leaf method is adopted, the content of Candidatus Liberibacter spp. pathogenic bacteria of half leaves which are not treated is detected through real-time fluorescent quantitation PCR, after the other half leaves are treated with thepotential biocontrol bacterium liquid, the content of the Candidatus Liberibacter spp. pathogenic bacteria of the other half leaves treated with the potential biocontrol bacterium liquid is detectedthrough the real-time fluorescent quantitation PCR, the efficacy of the potential biocontrol bacteria is obtained through a calculating formula, and the potential biocontrol bacteria high in control effects are screened to be used as target Candidatus Liberibacter spp. biocontrol bacteria. The method disclosed by the invention can be used for various hosts of the Candidatus Liberibacter spp., hasthe characteristics of being high in efficiency, quick and the like, and has important significance in biological control of the Candidatus Liberibacter spp..

Numerous plant microbes, including the vascular-limited Candidatus spp.—causal agents of citrus greening and potato zebra chip diseases—are non-culturable. The present disclosure relates, according to some embodiments, to compositions, methods and systems for culturing such organisms. For example, the present disclosure relates to methods for culturing, propagating, and characterizing fastidious vascular-colonizing microbes using a hairy root system (e.g., in vitro, in planta). The present disclosure relates, in some embodiments, to methods for cultivating a fastidious plant microbe including: contacting a plant (e.g., a tomato plant, a potato plant, a citrus plant) colonized by a fastidious plant microbe (e.g., Xylella fastidiosa, Candidatus Liberibacter spp.) with a suspension of R. rhizogenes under conditions that permit induction of hairy roots colonized with the fastidious plant microbe, and propagating the colonized microbial hairy roots.

The invention discloses a hybridoma cell strain 1D4 secreting an anti-CLas transport protein Ctp2 monoclonal antibody, the monoclonal antibody and application. The hybridoma cell strain 1D4 was preserved in China Center for Type Culture Collection (CCTCC) on November 26, 2019, the preservation number is CCTCC NO: C2019295, and the anti-CLas transport protein Ctp2 monoclonal antibody mAb 1D4 is secreted by the hybridoma cell strain 1D4. The anti-CLas transport protein Ctp2 monoclonal antibody mAb 1D4 secreted by the hybridoma cell strain 1D4 can recognize Candidatus Liberibacter spp. transportprotein Ctp2, and it is proved through an immunoblotting reaction that the monoclonal antibody mAb 1D4 can specifically recognize the transport protein Ctp2 of pathogenic bacteria CLas. The monoclonalantibody mAb 1D4 can be used for preparing preparations and drugs for inhibiting or controlling citrus Huanglongbing, and therefore, the citrus Huanglongbing is prevented and treated, and good popularization and application prospects are achieved.

The invention discloses a hybridoma cell strain 5A4 secreting an anti-CLas membrane protein Cmp1 monoclonal antibody, a monoclonal antibody and an application. The hybridoma cell strain 5A4 is collected in China Center for Type Culture Collection (CCTCC) on October 3, 2019, with a collection number of CCTCC NO: C2019219. The anti-CLas membrane protein Cmp1 monoclonal antibody mAb 5A4 secreted by the hybridoma cell strain 5A4 can specifically identify candidatus liberibacter membrane protein Cmp1; and an immunoblotting reaction proves that the monoclonal antibody mAb 5A4 can specifically identify the membrane protein Cmp1 of a pathogenic bacterium CLas in a susceptible plant leaf tissue. A preparation, a kit or a test strip for detecting liberobacter asiaticum can be prepared from the monoclonal antibody mAb 5A4, so that the field detection of the liberobacter asiaticum is achieved quickly and conveniently; and the monoclonal antibody mAb 5A4 has very good popularization and applicationprospects.

The invention belongs to the technical field of breeding of citrus virus-free seedlings and particularly relates to a method for controlling a pathogen of candidatus liberibacter in seedlings throughcomposite microorganism treatment and an application. Citrus Huanglongbing is a destructive disease in citrus production all over the world, and the pathogen of the citrus Huanglongbing is caused by difficult-to-culture candidatus liberibacter. According to the method, scions are provided for cultivation of virus-free seedlings or non-toxic or low-toxic shoot tip materials are provided for micro-shoot grafting and detoxification of shoot tips by reducing the pathogen concentration or controlling the pathogen of the candidatus liberibacter in citrus seedlings, so that the detoxification efficiency can be greatly improved, and effective means are provided for solving the bottleneck problem of virus-free improved variety breeding of citrus. Through high-strength application of a bacillus complex, a method for controlling and reducing the pathogen concentration of the Huanglongbing is found; an important foundation is laid for subsequent micro-shoot grafting and detoxification work; the targets of reducing the pathogen concentration of a maternal plant and improving the detoxification effect are achieved; and safe and healthy shoot tip scions are quickly provided for industry, therebyachieving the disease-free improved variety of the citrus.

The invention provides application of diaphorina citri ubiquitin-binding enzyme E2J2 gene in prevention and control of citrus huanglongbing, and belongs to the technical field of animal gene engineering. The invention provides the application of the diaphorina citri ubiquitin-binding enzyme E2J2 gene with a nucleotide sequence as shown in SEQ ID NO.1 in prevention and control of the citrus huanglongbing. A specific primer is designed to synthesize dsRNA, the silent E2J2 gene is interfered by RNA, the gene silencing efficiency of the gene is detected, the gene is subsequently placed on a branch infected with Candidatus Liberibacter asiaticum for feeding, and the influence of the gene on Candidatus Liberibacter asiaticum (CLas) infection is studied. By blocking the transmission path of the liberobacter asiaticum in the diaphorina citri instead of controlling the diaphorina citri, a new strategy is provided for effective prevention and control of the liberobacter asiaticum, and a new prevention and control thought is provided for controlling transmission of bacterial pathogens by means of vector insects.

The invention relates to compositions comprising Candidatus Liberibacter. The invention includes growth mediums, microbial cultures, methods, assays, and kits related to the compositions comprising Candidatus Liberibacter.

The invention relates to a method for screening a Candidatus Liberibacter resisting chemical through catharanthus roseus. The method includes the steps of cultivation of diseased catharanthus roseus plants, cutting regeneration of catharanthus roseus, optimization of cutting regeneration conditions of catharanthus roseus, chemical treatment and screening of the Candidatus Liberibacter resisting chemical. According to the method, the natural host catharanthus roseus for the plant Candidatus Liberibacter is taken as a test material for screening the Candidatus Liberibacter resisting chemical, the screening method is fast, easy and convenient to operate, and the effect of the chemical to be screened on resistance to Candidatus Liberibacter can be evaluated visually and accurately.

The invention discloses a method for analyzing the genetic diversity of citrus huanglongbing bacteria by using an SSR molecular marker primer system. The SSR molecular marker primer system includes 33 pairs of available primers for Las-SSR, of which 18 pairs are SSR core primers. The present invention proposes for the first time a method for analyzing the strain diversity of Ca.L.asiaticus based on SSR molecular markers combined with PAGE electrophoresis. The molecular basis is laid.