34 results about "Candidatus Liberibacter" patented technology

A method and compounds are described and claimed relating to plant defense inducer compositions and compounds, and to their use for improving certain aspects of plant and crop management, including treating plant disease, improving the ability of plants to defend against disease, reducing disease symptoms, treating HLB disease, minimizing crop yield decreases due to plant disease, improving crop productivity, and increasing crop quality. In particular, the compounds and compositions are useful for controlling and treating plant disease, for example Huanglongbing disease (HLB) and mitigating disease symptoms of HLB and Ca. Liberibacter infection in plants. Crop plants are contemplated for use with the invention, for example citrus trees. Methods according to embodiments of the invention involve treatment of affected plants by application (preferably by injection into the trunk, soil application or foliar spraying) of one or more compounds selected from β-aminobutyric acid or a salt thereof, 2-deoxy-D-glucose, salicylic acid or a salt thereof, oxalic acid. Typical methods involve application of a composition containing a botanically acceptable vehicle and β-aminobutyric acid. In certain methods, the plants used in the method are citrus plants, and the citrus plants are affected by or susceptible to Huanglongbing (HLB) disease or infection by Candidatus Liberibacter species.

The invention discloses a method for analyzing genetic diversity of a Candidatus Liberibacter by utilizing an SSR (simple sequence repeat) molecular marker primer system. The SSR molecular marker primer system comprises 33 pairs of Las-SSR available primers, 18 pairs of which are SSR core primers. The invention puts forward the SSR molecular marker based method for analyzing diversity of a Ca.L.asiaticus strain in combination with PAGE (polyacrylamide gelelectrophoresis). The method is simple and convenient, is high in analysis efficiency, and lays a molecular foundation for study of Candidatus Liberibacter molecular epidemiology.

The present invention relates to the use of dithiine-tetracarboximides of formula (I) for controlling selected bacterial harmful organisms in useful plants, wherein the bacterial harmful organisms are selected from the group consisting of Acidovorax avenae, Burkholderia spec., Burkholderia glumae, Candidatus Liberibacter spec., Candidatus Liberibacter asiaticus, Corynebacterium, Erwinia spec. (Dickeya, Pectobacterium carotovorum, Erwinia amylovora), Pseudomonas syringae, Pseudomonas syringae pv. actinidae, Pseudomonas syringae pv. glycinea, Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. lachrymans, Pseudomonas tumefaciens (=Agrobacterium tumefaciens), Streptomyces spp., Xanthomonas spp., Xanthomonas ampelina, Xanthomonas axonopodis, Xanthomonas axonopodis pv. citri, Xanthomonas axonopodis pv. glycines, Xanthomonas campestris, Xanthomonas campestris pv. musacearum, Xanthomonas campestris pv. pruni, Xanthomonas campestris pv. Viticola, Xanthomonas fragariae and Xanthomonas transluscens or Xylella fastidiosa. The present invention also relates to a method for controlling the selected bacterial harmful organisms in useful plants by treatment with a dithiine-tetracarboximides of formula (I).

DNA amplification methods using novel primers obtained from the novel genes for hyvI and hyvII s from the Candidatus Liberibacter asiaticus genome and useful for detecting Ca. L. species in plants and insect hosts.

The invention discloses a method for detoxification of citrus by utilizing microwaves, and belongs to the technical field of microwave application. The method is characterized in that during microwave pretreatment, the microwave irradiation frequency is 1.0 GHz-3.0 GHz, the power is 100-900 W, the microwave irradiation time is 5-20 seconds, then pausing is carried out for 10-30 seconds, repetitive operation is carried out 3-10 times, the temperature of each part of the citrus is increased to 40-45 DEG C, and the constant temperature is maintained for 30-60 seconds; then it is adjusted that the microwave irradiation frequency is 350 MHz-950 MHz, the power is 1,000-3,000 W, the microwave irradiation time is 3-10 seconds, then pausing is carried out for 20-60 seconds, repetitive operation is carried out 5-20 times, the temperature of each part of the citrus is increased to 50-55 DEG C, the constant temperature is maintained for 1-5 minutes, and citrus detoxification is carried out by utilizing the microwaves. The method for detoxification of the citrus by utilizing the microwaves can make each part of the citrus reach the fatal temperature of candidatus liberibacter more quickly and evenly, and a tree body is not affected; under the non-thermal effect of a microwave electromagnetic field, nucleic acid, protein and other molecules in the candidatus liberibacter are easy to denature and inactivate to achieve double sterilization effects; the detoxificated citrus through the microwaves are detected by adopting GB / T 28062-2011 <Real-Time Fluorescence PCR Detection Method of Candidatus Liberibacter>, and the negative conversion ratio of the candidatus liberibacter is greater than or equal to 82%. The method is an efficient, quick and environment-friendly citrus detoxification method.

Numerous plant microbes, including the vascular-limited Candidatus spp.—causal agents of citrus greening and potato zebra chip diseases—are non-culturable. The present disclosure relates, according to some embodiments, to compositions, methods and systems for culturing such organisms. For example, the present disclosure relates to methods for culturing, propagating, and characterizing fastidious vascular-colonizing microbes using a hairy root system (e.g., in vitro, in planta). The present disclosure relates, in some embodiments, to methods for cultivating a fastidious plant microbe including: contacting a plant (e.g., a tomato plant, a potato plant, a citrus plant) colonized by a fastidious plant microbe (e.g., Xylella fastidiosa, Candidatus Liberibacter spp.) with a suspension of R. rhizogenes under conditions that permit induction of hairy roots colonized with the fastidious plant microbe, and propagating the colonized microbial hairy roots.

The invention discloses a hybridoma cell strain 1D4 secreting an anti-CLas transport protein Ctp2 monoclonal antibody, the monoclonal antibody and application. The hybridoma cell strain 1D4 was preserved in China Center for Type Culture Collection (CCTCC) on November 26, 2019, the preservation number is CCTCC NO: C2019295, and the anti-CLas transport protein Ctp2 monoclonal antibody mAb 1D4 is secreted by the hybridoma cell strain 1D4. The anti-CLas transport protein Ctp2 monoclonal antibody mAb 1D4 secreted by the hybridoma cell strain 1D4 can recognize Candidatus Liberibacter spp. transportprotein Ctp2, and it is proved through an immunoblotting reaction that the monoclonal antibody mAb 1D4 can specifically recognize the transport protein Ctp2 of pathogenic bacteria CLas. The monoclonalantibody mAb 1D4 can be used for preparing preparations and drugs for inhibiting or controlling citrus Huanglongbing, and therefore, the citrus Huanglongbing is prevented and treated, and good popularization and application prospects are achieved.

The invention discloses a hybridoma cell strain 5A4 secreting an anti-CLas membrane protein Cmp1 monoclonal antibody, a monoclonal antibody and an application. The hybridoma cell strain 5A4 is collected in China Center for Type Culture Collection (CCTCC) on October 3, 2019, with a collection number of CCTCC NO: C2019219. The anti-CLas membrane protein Cmp1 monoclonal antibody mAb 5A4 secreted by the hybridoma cell strain 5A4 can specifically identify candidatus liberibacter membrane protein Cmp1; and an immunoblotting reaction proves that the monoclonal antibody mAb 5A4 can specifically identify the membrane protein Cmp1 of a pathogenic bacterium CLas in a susceptible plant leaf tissue. A preparation, a kit or a test strip for detecting liberobacter asiaticum can be prepared from the monoclonal antibody mAb 5A4, so that the field detection of the liberobacter asiaticum is achieved quickly and conveniently; and the monoclonal antibody mAb 5A4 has very good popularization and applicationprospects.

The invention discloses a citrus candidatus liberibacter and citrus canker pathogen dual detection kit and application thereof. A dual digital PCR quantitative detection agent composition for the citrus candidatus liberibacter and citrus canker pathogen is designed. The kit comprises a citrus disease sample DNA extraction agent, a digital PCR reaction agent, a citrus liberibacter asiaticus and citrus canker positive control sample template and a citrus liberibacter asiaticus and citrus canker negative control sample template. The kit has the advantages of being high in specificity, higher in impurity interference resistance, simple and fast when used for detecting the citrus candidatus liberibacter and citrus canker pathogen, the detection sensitivity is higher than that of an existing real-time fluorescence quantification PCR detection method by about 10 times, and the kit can be used for early-stage qualitative and quantitative detection of the citrus candidatus liberibacter and citrus canker pathogen and epidemiologic investigation.

The invention provides application of diaphorina citri ubiquitin-binding enzyme E2J2 gene in prevention and control of citrus huanglongbing, and belongs to the technical field of animal gene engineering. The invention provides the application of the diaphorina citri ubiquitin-binding enzyme E2J2 gene with a nucleotide sequence as shown in SEQ ID NO.1 in prevention and control of the citrus huanglongbing. A specific primer is designed to synthesize dsRNA, the silent E2J2 gene is interfered by RNA, the gene silencing efficiency of the gene is detected, the gene is subsequently placed on a branch infected with Candidatus Liberibacter asiaticum for feeding, and the influence of the gene on Candidatus Liberibacter asiaticum (CLas) infection is studied. By blocking the transmission path of the liberobacter asiaticum in the diaphorina citri instead of controlling the diaphorina citri, a new strategy is provided for effective prevention and control of the liberobacter asiaticum, and a new prevention and control thought is provided for controlling transmission of bacterial pathogens by means of vector insects.

The invention relates to compositions comprising Candidatus Liberibacter. The invention includes growth mediums, microbial cultures, methods, assays, and kits related to the compositions comprising Candidatus Liberibacter.

The invention relates to a method for screening a Candidatus Liberibacter resisting chemical through catharanthus roseus. The method includes the steps of cultivation of diseased catharanthus roseus plants, cutting regeneration of catharanthus roseus, optimization of cutting regeneration conditions of catharanthus roseus, chemical treatment and screening of the Candidatus Liberibacter resisting chemical. According to the method, the natural host catharanthus roseus for the plant Candidatus Liberibacter is taken as a test material for screening the Candidatus Liberibacter resisting chemical, the screening method is fast, easy and convenient to operate, and the effect of the chemical to be screened on resistance to Candidatus Liberibacter can be evaluated visually and accurately.

The invention discloses a method for analyzing the genetic diversity of citrus huanglongbing bacteria by using an SSR molecular marker primer system. The SSR molecular marker primer system includes 33 pairs of available primers for Las-SSR, of which 18 pairs are SSR core primers. The present invention proposes for the first time a method for analyzing the strain diversity of Ca.L.asiaticus based on SSR molecular markers combined with PAGE electrophoresis. The molecular basis is laid.