130 results about "Plant microbe" patented technology

The invention relates to an in-situ rain leaching repair system for contaminated soil. The in-situ rain leaching repair system at least comprises three parts, namely a rain collection part, a leaching repair part and a waste water treatment part, wherein the rain collection part is provided with water guiding pipes, a water collecting tank and canals, the leaching repair part is provided with an observation well, water collecting pipes and plants planted for the soil phytoremediation, the observation well is built in a middle-upper layer of contaminated soil, and the water collecting pipes are in the middle-lower layer; and the waste water treatment part is provided with a filter tank, a microorganism putting tank, an electric separation tank, a wind power generating set and the like, leaching waste water is firstly filtered by the filter tank, and is then repaired by the microorganism putting tank, and finally, the leaching waste water passes through the electric separation tank to process heavy metal so as to obtain the purified liquid. The in-situ rain leaching repair system establishes a complete contaminated soil simulated repairing system for the soil in an aeration zone, and combines repair technologies of leaching, plants, microorganisms, electric separation and the like. The in-situ rain leaching repair system has the characteristics of energy conservation, emission reduction and no secondary pollution, and is suitable for repairing the soil in a large-area aeration zone with little pollution.

The invention belongs to the field of ecological treatment of water environment, and discloses a method for ecologically purifying a black and odorous riverway water body. The ecological water body purification of the black and odorous riverway, realized by arranging an artificial wetland in a black and odorous riverway seriously polluted by city sewage and utilizing the comprehensive soil-plant-microorganism action, is less in investment, high in efficiency, stable in treatment effect, low in running cost, convenient to maintain, and has a good landscape ecology effect.

The present invention discloses a strain of bacillus, wherein the bacillus is Bacillusamyloliquefaciens WY11, and is preserved in the China General Microbiological Culture Collection Center (CGMCC) on October 11, 2012, and a preservation number is CGMCC No.6663. The bacterial agent adopting the bacillus as an active component is prepared by carrying out slant culture, seed culture and fermentation on the Bacillusamyloliquefaciens WY11 and adding an active agent. The preparation method for the bacterial agent adopting the bacillus as the active component comprises steps such as slant culture, seed culture, fermentation, liquid bacterial agent preparation and solid bacterial agent preparation. The invention further provides applications of the bacterial agent adopting the bacillus as the active component in plant root-knot nematode homicidal poisoning drug preparation. According to the present invention, the bacillus and the bacterial agent adopting the bacillus as the active component provide significant homicidal poisoning effects for plant root-knot nematodes; use is convenient; no toxic and harmful effect is generated to humans and animals; and plant microbe ecology flora can be regulated, and plant growth can be promoted.

The invention relates to a plant microorganism combination remediation soil and a shallow groundwater organic pollutant system, comprising a treating groove, plant remediation floating beds, an embedding immobilized microorganism processing film, a star-type aerating apparatus and a tail gas treatment device, wherein the system is remediated by the treating groove, the plant remediation floating beds, the embedding immobilized microorganism processing film, the star-type aerating apparatus and the tail gas treatment device; the treating groove is supported by a mesh supporting wall; the plantremediation floating beds are sampled mutually connected; the embedding immobilized microorganism processing film is fixed at a position 1.5m away from the bottom of the treating groove; and the star-type aerating apparatus is connected with the tail gas treatment device by a pipeline. The advantages are as follows: with relative simple design, the system is more convenient to carry out; the groundwater with organic pollutants has the characteristics of high efficiency, economy and non secondary pollution; the range for remediating underwater is wide; the variety of the removable pollutant isvery wide; and the system is applied to treating municipal sewage and domestic sewage.

The invention discloses a method for substantially increasing the dissolution of cell contents in animals, plants, and microbes. The method comprises the following steps: pre-grinding, soaking mixing, cavitation breaking, and backflow circulation, wherein the step of cavitation braking is to use a specially-designed machine to perform cavitation treatment to suspension liquid; and the machine, through a mechanical rotation mode, produces a great number of bubble clusters in the suspension liquid passing through the machine to generate strong cavitation impact force which can smash particles in the suspension liquid, accelerate the dissolution, homogenize further, and lower the system viscosity, so that cell walls or cell membranes are ruptured in order to promote the cell contents to flow out more easily, thereby substantially improving the quantity of the dissolution and the dissolution efficiency. At the same time, the invention discloses a device for initiating the cavitation phenomenon.

The invention discloses a method of bagging seed coat that utilizes composite active earth, adsorption and flocculent for seed, and the bagging-coat agent. Wherein active mineral earth, starch and chitosan, poyacrylamide composite, together with pesticide and chemical fertilizers are utilized for seed coat bagging.

The invention discloses a method for utilizing cadmium-resisting fungus aspergillus aculeatus for promoting festuca arundinacea to remedy cadmium contaminated soil, and relates to the technical field of plant-microorganism combined ecological remediation. The method mainly comprises the steps that firstly, cadmium-resisting fungus aspergillus aculeatus separated, purified and identified out of rhizosphere soil of dominant species in heavy metal contaminated soil is bred and inoculated into crushed crop straw to be prepared into a microbial remediation matrix, and the microbial remediation matrix is applied into cadmium contaminated soil; secondly, a festuca arundinacea lawn is planted in a sowing manner; thirdly, watering of a citric acid aqueous solution of a proper concentration and a proper quantity is carried out in batches; and fourthly, the rhizosphere microenvironment of festuca arundinacea is adjusted and controlled through citric acid to promote the interaction of cadmium-resisting fungus aspergillus aculeatus and a root system of festuca arundinacea so as to improve cadmium remediation efficiency. The problem of adaptability of screened-out microorganisms to the soil environment is solved; transferring of cadmium towards ground parts of plants is promoted, and the cadmium remediation efficiency is improved; and good environmental benefits, economic benefits and social benefits are achieved.

The invention discloses a reinforced denitrification dephosphorization pretreatment method and device for micropolluted water treatment artificial wetland. The method is suitable for advanced treatment of micropolluted lake (river) water, sewage treatment plant tail water and the like. The device is composed of a plant floating bed layer, a microbial bed filler, a filling granular layer and a supporting layer from top to bottom, and is provided with a back flush plant. The device disclosed by the invention fully utilizes the space in the device, and the plant-microbe can intercept, adsorb, absorb, enrich and decompose part of pollutants; the zeolite-steel slag-limestone filler combination can effectively remove nitrogen, phosphorus and other pollutants in water, thereby ensuring that all the water-quality indexes of the effluent water of the subsequent artificial wetland can achieve the related high-quality water application standard; and the back flush process is beneficial to enhancing the purification efficiency and prolonging the service life of the device.

A composition for treating waters, e.g. ballast water or injection water for oil recovery, to kill in-situ aquatic invasive species comprises at least one biocide capable of killing both animal and plant micro-organisms. The at least one biocide preferably comprises Brilliant Green, Gentian Violet, and/or erythrosine, and a wetting agent or detergent-like compound such as CTAB or CTAC. The invention also relates to a system for treating ballast water in situ comprising means for injecting a composition for treating ballast water; means for measuring the flow rate or amount of ballast water to be treated; means for controlling the dosing of the composition; and means for storing or receiving the composition. The invention also relates to a method of detecting viable aquatic organisms in ballast water in situ comprising detecting metabolism in viable micro-organisms in ballast water and, therefore, measuring the efficacy of any treatment.

The invention discloses denitrifying bacteria and an aquatic plant-microbe combined repair method using the same. The denitrifying bacteria LR were collected in China Center for Type Culture Collection on May 3rd, 2011, and the collection number of the strains is CCTCC NO: M2011159. The plant-microbe combined repair method for a nitrogen eutrophic water body comprises the following steps of: planting cyperus alternifolius in the nitrogen eutrophic water body, and inoculating the denitrifying bacteria LR. The denitrifying bacteria LR and the cyperus alternifolius or cyperus alternifolius root exudates are combined for repairing the nitrogen eutrophic water body, and the strains LR has a promotion effect of repairing the nitrogen polluted water body through the cyperus alternifolius. The method for repairing the nitrogen eutrophic water body by combining the denitrifying bacteria LR and the cyperus alternifolius or the cyperus alternifolius root exudates is particularly suitable for treating the nitrogen eutrophic water body in nature.

A plant-microbe joint directional restoring penchlorol contaminated soil starts from selecting specific high degrading PCP degrading bacteria, then testing them and choosing optimum cultivating conditions. With soils of different degree of contamination, it can be used for potting plant suaeda glauce testing soil. Test the PCP left quantity in different growing stage of the suaeda glauce through sampling of the soil. It has the highest PCP degrading rate, with low density PCP (5mg/kg) reaching 94.73%. Combining of the specific PCP degrading bacteria and planting saeda glauce, restoring effect of soil is far bigger than implementing either one of them, reaching optimum PCP degrading result.

Disclosed herein are systems, methods, and devices for determining plant-microbe interactions. In some embodiments, the device comprises: a root chamber configured to allow a root of a plant to grow, wherein the root chamber is connected with: an inlet channel for introducing a medium into the root chamber, an outlet channel for collecting plant exudates and metabolites, and a plant reservoir for the plant shoot of the plant to grow.

The invention relates to wettable Bacillus methylotrophicus powder and a preparation method and application thereof.The wettable Bacillus methylotrophicus powder is prepared from a strain with the preservation number being CGMCC No.11838 according to the steps of seed activation, seed cultivation, ferment cultivation, drying, compounding and the like.The application of the wettable Bacillus methylotrophicus powder to preparation of medicines for controlling potato common scab is involved.The wettable Bacillus methylotrophicus powder, a microbial agent, is high in biological activity, remarkably effective in inhibition of the potato common scab, good in environmental compatibility, capable of regulating ecosystems of plant microbes, nonhazardous to people and livestock and convenient to use.

The invention relates to a red-spotted stonecrop root lead-resistant strain Providence bacterium (Providencia sp.) BPb7. In particular, the invention relates to the lead-resistant strain Providence bacterium (Providencia sp.) BPb7 separated from a red-spotted stonecrop root. The lead-resistant strain Providence bacterium (Providencia sp.) BPb7 is preserved in the China General Microbiological Culture Collection Center on December 1, 2014; and the preservation serial number is CGMCC No.10085. The lead-resistant strain Providence bacterium (Providencia sp.) BPb7 is a gram negative bacterium and aerobasilus, pili exist, and capsules do not exist. A bacterial strain has a higher tolerance on the heavy metal lead, and the red-spotted stonecrop root lead-resistant strain Providence bacterium (Providencia sp.) BPb7 has the practical significance and the important engineering application value on heavy metal contaminated soil recovering through plants and microorganisms jointly.

The invention relates to a sedum rhizosphere cadmium-resistant bacillus sp. BCd5 strain and its screening method. The invention specifically relates to a sedum rhizosphere cadmium-resistant bacillus sp. BCd5 strain which has been preserved in the China General Microbiological Culture Collection Center (CGMCC) on December 1st, 2014 with the preservation number being CGMCC NO. 10077. 16S rDMA gene sequence of the strain is as shown in the Figure 1. The strain is a Gram-negative bacterium, aerobasilus having flagellum and capsule. The strain has high resistance to cadmium and has practical significance and important engineering application value in plant-microbial remediation of cadmium-contaminated soil.

The invention relates to hylotelephium erythrostictum rhizosphere cadmium-resistant Cupriavidus sp. BCd1, and a screening method and applications thereof, and more specifically relates to the cadmium-resistant Cupriavidus sp. BCd1 obtained via separation from hylotelephium erythrostictum rhizosphere. The hylotelephium erythrostictum rhizosphere cadmium-resistant Cupriavidus sp. BCd1 is stored in China General Microbiological Culture Collection Center on 1th December 2014, and preservation number is CGMCC No.10080. The 16S rDNA gene sequence is represented by graph 1. The hylotelephium erythrostictum rhizosphere cadmium-resistant Cupriavidus sp. BCd1 is Gram negative, belongs to aerobic coccobacillus, possesses flagellum and capsule, possesses relatively high resistance on heavy metal cadmium, and possesses practical significance and high engineering value in plant-microorganism combined remediation of cadmium heavy metal polluted soil.

The invention relates to a plant-microorganism combined remediation method for a moderate and mild lindane-cadmium combined polluted farmland. Cadmium hyperaccumulators and crops are planted on polluted soil, a compound fertilizer and a biochar base fertilizer are applied, the carbon-nitrogen ratio of the soil is regulated while the pH value of the soil is reduced by using the biochar base fertilizer, the absorption of the hyperaccumulators to pollutants isenhanced while the biomass of the hyperaccumulators is improved, the growth of the hyperaccumulators is not influenced by concentration aggregation of the pollutants, pseudomonas and arbuscular mycorrhizal fungi are prepared into a mixed microbial inoculum, and the resistance of the hyperaccumulators is improved; and economic crops with root systems deeper than those of the hyperaccumulators and the hyperaccumulators are selected for intercropping/relay intercropping, the soil fertility is improved, the growth environment of the hyperaccumulators is adjusted, the remediation efficiency of the hyperaccumulators on lindane and cadmium is further enhanced, and the purpose of remediation while production is achieved.

The invention specifically relates to enteroaerogen HBRM-86 and applicaton of the same as a rubber tree growth-promoting rhizobacterium strain, belonging to the technical field of plant microbes. According to the invention, ACC deaminase is used as a main index; the enteroaerogen HBRM-86 is obtained through elaborate screening and preserved in China Center for Type Culture Collection; and experimental results prove that the screened enteroaerogen HBRM-86 can effectively function as a growth-promoting rhizobacterium strain, benefits growth of the seedlings of a rubber tree and is favorable for increasing the output of natural rubber.

The invention provides a method for improving water quality by using plant-microbe coupled ecological floating islands. The method is characterized in that self purification capacity of a water ecosystem is promoted by constructing a plant-microbe coupled bio-community. The method comprises the following steps: an ecological enclosure is arranged along a river channel coast, so that the exchange rate between coastal water body and an open water area is reduced; a composite ecological floating island is arranged, and aquatic plants are planted on a floating bed; elastic three-dimensional filleris suspended under and around the floating bed; and an immobilized compound water purification agent is added to the root of the aquatic plants. According to the method, the repairing effect is obvious, the maintenance is convenient, and secondary pollution to water body is not caused during the treatment process; and the method is especially suitable for water body treatment of water flowing river channels with cement revetment.

The invention belongs to the technical field of plant microbes, and in particular relates to serratia marcescens HBRM-16 and an application of the serratia marcescens HBRM-16 as a bacterial strain for promoting the growth of the roots of a rubber tree. According to the invention, ACC deaminase is taken as the main index, a serratia marcescens HBRM-16 strain is obtained through elaborate selection, and the serratia marcescens HBRM-16 strain is preserved in the China Center for Type Culture Collection (CCTCC); the experiment proves that the selected serratia marcescens HBRM-16 can be effectively taken as the strain for promoting the root growth, so that the growth of rubber tree seedlings is promoted, and the yield of natural rubber is improved.

The invention relates to a method for remediating heavy metal-contaminated soil by combining sludge biochar immobilized bacteria with plants. The method comprises the following steps: screening plants; screening and separating bacteria resistant to various heavy metals; separating out bacteria capable of dissolving phosphorus, fixing nitrogen, secreting plant growth hormone, secreting siderophore,promoting biomass accumulation of plants, root development and growth hormone secretion; loading and fixing the bacteria on sludge biochar; repairing soil; and the like. According to the invention, the sludge biochar is used for immobilizing strains, and microorganisms are protected by the biochar and can be slowly released in soil, so the survival and field planting capacity of the microorganisms in plant bodies and rhizospheres is effectively improved, the biomass of plants is greatly improved, and the heavy metal content of soil is effectively reduced; and meanwhile, root extraction of remediation plants is promoted, and the goal of remediation of heavy metal pollution of the soil by a combination of plant-microorganism-carbon-based material is achieved.

The invention discloses a method for screening control enzyme of microalgae adipopexis. The method comprises the steps of (1) establishing a salt environment under the manual simulation of a laboratory, and culturing salt-sensitive chlorella and salt-resistant chlorella; (2) analyzing fluorescence difference gel electrophoresis proteomics of the whole protein of algae cells; and (3) screening and identifying key control enzyme of the microalgae adipopexis. The method disclosed by the invention has the advantages that due to the same key control enzyme for inducing grease synthesis in different algae plants by adopting the salt-resistant chlorella and the salt-sensitive chlorella as screening objects under the salt environment, the effective control for anabolism of microalgae grease with different energies and the construction of high-yield grease gene engineering microalgae are excepted to be realized; the error between gel and gel in the traditional two-dimensional electrophoresis (silver staining and coomassie-brilliant-blue staining) is eliminated, and the accuracy and the repeatability are improved; and simultaneously, an established general technology system for discovering and identifying the key control enzyme of the grease synthesis can be applied to the fields of metabonomics analysis of animals, plants, microorganisms and fungus in a radiation manner.

The invention relates to the technical field of ecological cycle, in particular to a fish and vegetable symbiotic system. The invention provides the fish and vegetable symbiosis system, which is characterized in that specially cultured autotrophic bacteria are added and conveyed to a fish pond for fish culture, aquaculture water is periodically pumped and conveyed into a cultivation system, and the aquaculture water returns to a clean water pond after being absorbed by vegetables and filtered by a sewage discharge pond, so that ecological circulation is realized, and waste resource utilizationis realized; during the period, the autotrophic bacteria decompose a large amount of ammonia nitrogen generated by aquaculture into nitrite, and then the nitrite is decomposed into nitrate by nitrifying bacteria to be absorbed and utilized by plants. The fish and vegetable symbiotic system provided by the invention enables animals, plants and microorganisms to achieve ecological balance, is a sustainable, circulating, pollution-free and zero-emission low-carbon production mode, can realize large-density culture of fish, and meanwhile, planted crops are high in growth vigor, high in nutrition,simple in system structure, high in integration, convenient to clean and suitable for popularization, and huge popularization values and development prospects are realized.

The invention provides a method for rapid remediation of contaminated soil by MP matrix technology. The method utilizes microorganisms and plants to remediate contaminated soil synergistically so as to achieve the purpose of rapid remediation. The method provided by the invention comprises the steps of: 1) conducting sampling and detection on contaminated soil; 2) selecting appropriate plants forplanting according to the detection result and plant properties to remediate the contaminated soil; and 3) selecting microorganisms for inoculation into the soil according to the detection result andmicrobial properties. The method provided by the invention is a fast and green plant-microorganism combined method for remediation of contaminated soil. The method is simple in operation, has strong adaptability and inclusiveness, and has good treatment effect.