46 results about "Plant microbe" patented technology

The invention provides a low-carbon modular artificial wetland system and a carbon sink collaborative purification method thereof, and relates to the technical field of artificial wetlands, the low-carbon modular artificial wetland system comprises a modular box body, and a water inlet layer, a carbon sink layer and a purification layer which are sequentially arranged in the modular box body from top to bottom; the water inlet layer is used for filtering suspended matters through coarse sand; the carbon sequestration layer is used for adsorbing carbon dioxide through biochar and carbon sequestration microorganisms, and meanwhile, the carbon sequestration capacity is enhanced through the plant-microorganism synergistic effect; the purification layer is used for purifying a water body through an iron-based phosphorus removal material and a zeolite denitrification filler. The technical problems of low-carbon material selection, carbon sink potential excavation, renewable energy source coupling and greenhouse gas emission inhibition of an existing constructed wetland system are solved, the technical effects of efficient sewage purification and carbon emission reduction are achieved, and a carbon emission reduction closed loop of the whole life cycle is achieved.

The invention relates to the technical field of heavy metal contaminated soil remediation, in particular to a plant-microorganism-passivator synergistic remediation method for a heavy metal contaminated farmland, which comprises the following steps: step 1, constructing a soil digital twinborn model; step 2, intelligent decision making and verification of a collaborative restoration scheme; 3, soil pretreatment before remediation; step 4, hierarchical collaborative restoration and dynamic regulation and control; 5, performing long-term verification on the repair effect and performing model iteration; physical heat treatment is used as strengthening pretreatment, the occurrence form of heavy metal is changed through high temperature, biomass charcoal is generated in situ, passivation immobilization-microbial strengthening-plant extraction three-stage collaborative operation is carried out according to the time sequence, pollutants are rapidly locked through a passivating agent, rhizosphere immobilization is strengthened through functional microorganisms, plant growth is promoted, and the quality of plants is improved. And the hyperaccumulator finally removes the heavy metals from the soil, so that the unification of short-term control and long-term reduction is realized.

The present disclosure provides non-intergeneric remodeled microbes that are able to fix atmospheric nitrogen and deliver such to plants in a targeted, efficient, and environmentally sustainable manner. The utilization of the taught microbial products will enable farmers to realize more productive and predictable crop yields without the nutrient degradation, leaching, or toxic runoff associated with traditional synthetically derived nitrogen fertilizer, by mitigating or eliminating the need for exogenous nitrogen-containing fertilizers. The remodeled microbes have unique colonization and nitrogen fixation abilities, which enable the microbes to deliver nitrogen to a cereal plant in a spatially targeted (e.g. rhizospheric) and temporally targeted (e.g. during advantageous stages of plant's life cycle) manner. The microbes are able to replace the standard agricultural practice of sidedressing and enable a more environmentally sustainable form of farming. The present disclosure also provides methods of using non-intergeneric remodeled microbes, for example, to fix atmospheric nitrogen by reducing or eliminating the need for exogenous nitrogen-containing fertilizers, to increase yield, and to reduce infield variability in the yield.

The invention provides a method for strengthening plant microorganism combined remediation of heavy metal contaminated soil, and relates to the technical field of soil remediation. The method for strengthening the plant and microorganism combined remediation of the heavy metal contaminated soil comprises the following steps: a) selecting and planting pioneer plants with tolerance to target heavy metal; b) applying a compound microbial agent containing at least two functional microorganisms, wherein the functional microorganisms comprise plant growth-promoting bacteria and microorganisms forming a symbiotic relationship with plants; c) loading the functional microorganisms on a biochar carrier, and applying the biochar carrier to soil; d) selectively adding an exogenous chelating agent according to the effective state concentration of the heavy metals in the soil; and e) selectively carrying out genetic engineering modification on the functional microorganisms or pioneer plants according to repair requirements. The technology is high in flexibility, optimization combination can be carried out according to different heavy metal types, combined pollution and soil conditions, and the advantages of high efficiency, environmental safety and long-term sustainability of the remediation process are achieved.

The invention discloses a water quality purification system based on a heterogeneous catalytic material and an ecological floating bed. The water quality purification system comprises a floating bed device, a purification device, a fixing device and a water moving trolley, the floating bed device comprises a floating bed carrier and aquatic plants planted on the floating bed carrier; root systems of the aquatic plants extend into the water, and microorganisms are attached to the root systems; the purification device is arranged on the floating bed carrier, is in contact with water flow and comprises a plurality of purification assemblies distributed on the floating bed carrier at intervals, and each purification assembly comprises a purification bag suspended on the floating bed carrier and a heterogeneous catalytic material loaded in the purification bag; the fixing device is arranged on the floating bed carrier and fixes the floating bed carrier on the water surface of a target area; the water moving trolley is connected with the floating bed carrier and drives the floating bed carrier to the water surface of the target area; according to the water quality purification system disclosed by the invention, a material-plant-microorganism three-in-one synergistic purification framework is constructed, and the potential of an ecological floating bed in the aspects of sustainable pollution control and phosphorus resource recovery is remarkably enhanced.

The invention provides a submerged plant-microorganism synergistic lake ecological restoration system and application thereof, and belongs to the technical field of water body ecological restoration. The submerged plant-microorganism synergistic lake ecological restoration system comprises a functional complementary symbiotic combination module and a three-dimensional microenvironment enhancement module, the functional complementary symbiotic combination module comprises a submerged plant community, a microbial community, an inducer and a nutritive salt regulating agent; the three-dimensional microenvironment enhancement module comprises a rhizosphere microenvironment optimization unit, a water body microenvironment regulation and control unit and a substrate microenvironment improvement unit. According to the system, by constructing the functional complementary symbiotic combination module and the three-dimensional microenvironment enhancement module, efficient coupling and synergistic interaction of submerged plants and microbial communities are achieved, and lake water quality and ecology can be systematically and comprehensively improved.

This disclosure relates to a method for improving the colonization efficiency of rhizosphere growth-promoting bacteria using nanomaterials and a magnetic interaction system. The method includes: S1, mixing modified iron oxide nanoparticles with a suspension of rhizosphere growth-promoting bacteria and then conducting a first culture to obtain modified rhizosphere growth-promoting bacteria; S2, applying a dispersion of magnetic iron oxide nanoparticles to the roots of a plant for a second culture to obtain modified plants; S3, applying the suspension of the modified rhizosphere growth-promoting bacteria to the roots of the modified plants for a third culture; wherein the modified iron oxide nanoparticles are cationic polymer-modified iron oxide nanoparticles. This method constructs a plant-microorganism magnetic interaction system, improving the colonization ability of rhizosphere growth-promoting bacteria in plant roots, and also enhancing the nitrogen-fixing ability of the rhizosphere growth-promoting bacteria, effectively promoting plant growth.

The invention discloses a method for behavioral growth of plants and microorganisms under catalysis of nitrogen-based nutrition in the crossing field of biotechnology, agriculture and microbial engineering. Nitrogen-based substances of an organism are extracted to prepare a targeted nutrition carrier, and nutrition and environmental parameters are accurately regulated and controlled in combination with physical and chemical means, so that the limitation of a traditional technology is broken through, and low-grade plants and specific microorganisms can grow according to preset patterns (such as human body images). Compared with the traditional technology, the method does not need high-cost external intervention, has the functions of resource circulation, art expression and ecological restoration, and provides an innovative technical path for commemorative art, ecological restoration and personalized gardening development.

The invention discloses a chenopodium ambrosioides seed endophytic fungus Alternaria alstroemia HZ132. The endophytic fungus Alternaria alstroemia HZ132 is preserved in Guangdong Microbial Culture Collection Center with the preservation number of GDMCC No. 66913, and the preservation number of the endophytic fungus Alternaria alstroemia HZ132 in Guangdong Microbial Culture Collection Center is GDMCC No. 66913. The strain has strong capabilities of solubilizing phosphorus, producing indoleacetic acid, producing siderophores and resisting cadmium, is inoculated with HZ132 under cadmium stress, can significantly promote plant growth and enhance cadmium resistance of plants, and has important significance in plant-microorganism combined remediation of heavy metal contaminated soil.

The invention discloses a soil heavy metal remediation method based on indigenous plant-microorganism-charcoal synergy, and belongs to the technical field of soil remediation. The soil heavy metal remediation method comprises the following steps that S1, a heavy metal polluted land parcel is selected, and the variety and content of heavy metal in the land parcel are analyzed; s2, selecting crop rotation plants according to heavy metal pollution conditions; s3, preparing an activated carbon-functional microbial agent; s4, cleaning the heavy metal polluted land parcel, applying an activated carbon-functional microbial agent, and ploughing; s5, sowing seeds of the selected plants, and performing field management, harvesting and crop rotation. A'indigenous plant-functional microorganism-modified charcoal 'synergistic remediation system is constructed, through efficient synergism of targeted plant rotation, acid modified charcoal fixation and compound microorganism activation, the total amount and the effective state content of various heavy metals in soil can be remarkably reduced, meanwhile, the physical and chemical properties of the soil are improved, and the soil remediation effect is improved. And efficient, green and sustainable treatment of the heavy metal contaminated soil is achieved.

The application provides a method for repairing mine wasteland by synergistically activating shield slurry and sludge by plants and microorganisms, injecting ozone into domestic sludge to obtain modified sludge, uniformly mixing the modified sludge, the shield slurry, biological bacterial fertilizer and arbuscular mycorrhizal preparation at a dry weight ratio of (4-5):(1-2):(0.2-0.3):(0.2-0.3) to obtain mixed slurry, performing pressure filtration dewatering treatment on the mixed slurry, and controlling the water content to be 30-50% to obtain mixed dry material, adding tailing sand and pioneer grass seeds to the mixed dry material, irrigating and cultivating at room temperature for 7 days to obtain mixed plant sludge cake, crushing the mixed plant sludge cake to obtain ecological restoration soil, adding ecological curing agent to the ecological restoration soil to obtain cured restoration soil, adding grass seeds to the cured restoration soil to obtain modified restoration soil, adding grass seeds to the ecological restoration soil after laying the ecological restoration soil on the flat section of the mine wasteland, spraying and sowing the modified restoration soil on the slope section of the mine wasteland, repairing the mine wasteland by the ecological restoration soil and the modified restoration soil, and realizing ecological environment restoration succession cycle.

The invention discloses a biomass charcoal material-based modular artificial wetland water quality stabilizing system, which comprises a plurality of independent wetland modules, the independent wetland modules are transversely or longitudinally connected with one another through quick connectors, and each independent wetland module comprises a vertical layered water quality purification system, a self-adaptive hydraulic regulation and control system and an energy self-supply system; the vertical layered water quality purification system comprises a water supply layer, a matrix layer and a plant-microorganism layer, the matrix layer comprises a magnetic biochar layer, a middle slow-release microorganism carrier layer and a bottom permeable conductive concrete layer, and all the layers are integrated with optical fiber sensors and are connected to a central control system; the plurality of bionic hydraulic structures are arranged in the independent wetland modules to form a spirally rising water flow path; through the cooperation of the vertical layered water quality purification system, the self-adaptive hydraulic regulation and control system and the energy self-supply system, the functions of water quality purification and energy utilization are realized, so that the stability of the water quality of the wetland is ensured.

The invention discloses application of adenine in promoting corn growth, and belongs to the technical field of microorganisms and agriculture. The invention finds and verifies that adenine is a key signal molecule for driving the assembly of corn genotype-specific microbiome for the first time, and exogenous addition can directly and efficiently convert a microbial community structure to a beneficial direction; exogenous application of adenine can significantly promote increase of root length, fresh weight and dry weight of corn seedlings and increase starch content, which indicates that the adenine optimizes the physiological status and carbon assimilation efficiency of plants. Adenine is a natural purine substance, is environment-friendly, is different from chemical fertilizers and pesticides, and accords with the development direction of green sustainable agriculture. According to the invention, a new plant-microorganism interaction mechanism is disclosed from a causal chain of'plant signal-microorganism response-phenotypic feedback ', and a brand new theoretical basis and a technical path are provided for crop micro-ecological regulation and control.

The invention discloses a method for purifying micropterus salmoides culture tail water, and belongs to the technical field of aquaculture wastewater treatment. The purification method comprises the following steps: planting barley, hemp peas and vanilla in micropterus salmoides culture tail water, and adding a carrier immobilized complex microbial inoculant into the micropterus salmoides culture tail water before planting the barley, the hemp peas and the vanilla. A high-efficiency plant-carrier immobilized complex microbial inoculant synergistic purification system is constructed by cooperatively putting a carrier immobilized complex microbial inoculant into a water body and planting specific plants (barley, hemp peas and vanilla). Through the triple synergistic effect of the plants, the microorganisms and the carrier, the problems of low efficiency, incomplete nitrogen and phosphorus removal, poor system stability and the like in the traditional single plant purification technology are effectively solved, so that stable and efficient purification of the micropterus salmoides culture tail water with high pollution load is realized.

The application belongs to the technical field of agricultural microbiology and plant protection, and discloses application of an immunosuppressive active substance, and aims to solve the problem that there is no immunosuppressive substance for effectively suppressing plant immunity, improving the colonization efficiency of microorganisms on crops, and improving the biological prevention and control effect in the prior art. The immunosuppressive substance comprises the following applications: application in inhibiting plant immunity, application in promoting plant microbial colonization, application in plant disease biological prevention and control, and application in preparation of agricultural microbial agents, biological prevention and control agents and / or growth promoting agents. The application is suitable for crop health and sustainable agricultural production, and can achieve the effect of plant disease biological prevention and control.

The invention discloses a nitrogen-fixing, phosphorus-dissolving and potassium-promoting multifunctional microbial fertilizer and a preparation method thereof, and relates to the technical field of microbial fertilizers, the nitrogen-fixing, phosphorus-dissolving and potassium-promoting multifunctional microbial fertilizer comprises a multifunctional microbial agent core and a PVA composite hydrogel coating layer; the preparation method comprises the following steps: 1) preparing the multifunctional microbial agent core; (2) preparing PVA (polyvinyl alcohol) composite hydrogel; 3) coating process; according to the invention, through molecular design, the coating layer can accurately identify salicylic acid signals secreted by a root system, and specific degradation occurs in a root-fertilizer interface micro-domain, so as to realize on-demand supply and spatial targeting of nutrients; the mechanism greatly avoids soil fixation, leaching loss and invalid competition of microorganisms; meanwhile, the system integrates nitrogen-fixing, phosphorus-dissolving and potassium-promoting multifunctional microbial agents, and combines the immune excitation function of salicylic acid, so that a plant-microorganism synergistic rhizosphere micro-ecology is constructed, and the unification of weight-losing synergism and green sustainable development is realized.

The invention relates to a method for improving colonization efficiency of growth-promoting rhizobacteria by utilizing a nano material and a magnetic interaction system. The method comprises the following steps: S1, mixing modified ferroferric oxide nanoparticles with a bacterial suspension of growth-promoting rhizobacteria, and carrying out first culture to obtain modified growth-promoting rhizobacteria; s2, applying the dispersion liquid of the magnetic ferroferric oxide nanoparticles to the roots of the plants for secondary culture to obtain modified plants; s3, applying the bacterial suspension of the modified growth-promoting rhizobacteria to the root of the modified plant for third culture; wherein the modified ferroferric oxide nano particles are nano ferroferric oxide modified by a cationic polymer. According to the method, a plant-microorganism magnetic interaction system is constructed, the colonization capacity of the growth-promoting rhizobacteria at plant roots is improved, meanwhile, the nitrogen fixation capacity of the growth-promoting rhizobacteria is improved, and plant growth is effectively promoted.

The invention provides a processing and using method of an arbuscular mycorrhizal fungus soil conditioner, which utilizes a plant-microorganism-soil symbiotic structure-activity relationship, and recruits and assembles local dominant microorganisms in a natural environment by screening and planting host plant dominant mycorrhizal fungi, so that the arbuscular mycorrhizal fungus soil conditioner is obtained. A rhizosphere life community which is beneficial to plant growth and perirhizosphere soil health is formed, and is separated, induced and purified to manufacture compound microorganisms, fertilizers and soil amendment products.

The invention provides application of cellooligosaccharide in inducing plant PTI to promote rhizobium recruitment and symbiotic nodulation, and belongs to the technical field of plant-microorganism interaction. The invention provides an application of cellooligosaccharide in inducing plant innate immunity, cellooligosaccharide with structure specificity is used for inducing PTI signals, and accurate targeting recruitment of rhizobium is carried out to improve the nodulation nitrogen fixation and growth performance of plants. The invention further provides a method for promoting plant growth, the compatibility state of plant growth and immunity is maintained under PTI activation only through PTI stimulation of the cello-oligosaccharide, and the nodulation efficiency and yield of crops are effectively improved by establishing a positive feedback model of PTI immune activation-microbial interaction-nutrition enhancement-growth promotion. According to the method, the in-situ symbiotic rhizobium can be accurately enriched without supplementing an exogenous microbial agent, and the method is an in-situ microorganism regulation and control method which is high in ecological adaptability and high in field generalizability.

The utility model provides a purification device for lake water quality water ecology, which relates to the technical field of water quality purification, and comprises a buoy, a plurality of mounting and adjusting components, a plurality of mounting plates, a floating plant culture cylinder, a microbial carrier box, a submerged plant planting box and a connecting component, the floating plant cultivation barrel can float in an area where lake water pollution is serious through the buoy, the positions of the plant cultivation barrel and the microorganism carrier box can be adjusted, and the submerged plant planting box can be moved to the area where lake water pollution is serious. Microorganisms in a floating plant microorganism carrier box in the floating plant culture cylinder and submerged plants in a submerged plant planting box can directly purify lake water, and the purification speed of a lake water pollution area is higher through the combined action of the submerged plants, the floating plants and the microorganisms; and the purification effect is better.

The application discloses a kind of immobilized microbial biological seed carrier product and its preparation method, preparation method includes the following steps: first, diatomite is used to adsorb petroleum hydrocarbon-degrading bacteria, then vacuum freeze-drying into powdered bacterium agent, secondly, powdered bacterium agent is mixed with coating agent high molecular water-absorbing resin, bentonite, polyvinyl alcohol, gibberellic acid uniformly, with seed and coating agent raw material 1:1 is coated, color rape seed is placed in the rotating disc of coating machine with uniform speed, quantitative binder solution is sprayed, it is mixed granulation under the rotation speed of 90r / min, continuous alternate operation several times to the coating agent is evenly wrapped on rape seed, finally idle 2min to increase strength, obtain seed carrier immobilized microbial product.The carrier material is green and environment-friendly, low in cost, simple in operation, and can be mass-produced, and lays a certain foundation for strengthening plant-microbe collaborative remediation.

Modified synthetic peptides exhibiting improved antimicrobial activity in comparison to wild-type peptide sequences as well as compositions and plant parts which have been treated with the peptides are provided. Methods of using the modified peptides to control microbial infections of plant and animal subjects are also provided.

The invention relates to the technical field of farmland improvement. The invention provides an ecological improvement method based on improvement of land fertility of reclaimed cultivated land. The ecological improvement method comprises the following steps: (1) leveling a field; (2) field irrigation; (3) sowing the cichorium intybus; (4) chicory heading and covering; (5) putting earthworm seedlings; (6) chicory heading, covering and the like. According to the invention, a cichorium intybus-earthworm-microbial inoculum composite ecological system is utilized to improve the physicochemical properties of the reclaimed soil, improve the soil fertility of a plough layer and promote the restoration of a soil ecological system of the reclaimed land. According to the scheme, a set of'animal-plant-microorganism 'composite ecological system is constructed by adopting a'chicory-earthworm-bamboo fiber fungicide' ecological system, so that the improvement and fertilization effects of the reclamation land are remarkably improved.

The invention relates to an integrated plant microbial fuel cell system, and belongs to the technical field of microbial fuel cells. The system comprises a plurality of reactor units, each reactor unit is provided with a cathode and an anode, and the reactor units are electrically connected in series to form a voltage output system. Series connection is achieved through a special series module, the module integrates a power transmission guide rail and a clamping groove structure, and the current path and the voltage superposition efficiency are optimized. The system supports modular expansion, output voltages of the multiple reactor units are superposed through series connection, stable voltage output is achieved, and the system is suitable for energy supply of outdoor low-power-consumption equipment. According to the invention, the problems of low power generation efficiency, unreliable connection, poor expansibility and the like when the existing PMFC systems are connected in series are solved, and the system has the advantages of high output voltage, stable connection and easy maintenance.

The invention discloses a method for repairing organic matters in alpine desertified soil by utilizing female salix psammophila symbiotic arbuscular mycorrhizal fungi, which comprises the following steps: removing impurities in the alpine desertified soil, inoculating the surface of the soil with an arbuscular mycorrhizal fungi inoculant, and then covering and compacting the soil; the single sex salix psammophila is planted on the inoculated soil, and the arbuscular mycorrhizal fungus inoculant comprises near glomus amari or / and sporocyst mucilaginosum. The problems that in the prior art, nitrogen circulation is weak, male and female mixed planting mycorrhiza infection is low, accurate matching is lacked, and carbon sequestration is slow are solved, an efficient plant-microorganism symbiotic system is established by combining arbuscular mycorrhizal fungi with different sexes of salix psammophila, the synergistic interaction effect is remarkable, the organic carbon content of soil is systematically increased, and the soil quality is improved. The ecological function of desertified soil is restored, so that the growth of the salix brachypoda is promoted; the bidirectional substance and energy exchange forms a virtuous cycle, and the productivity and stability of an ecological system are fundamentally improved.

This invention discloses a method for purifying wastewater from California bass farming, belonging to the field of aquaculture wastewater treatment technology. The purification method includes the following steps: planting barley, peas, and herbs in the California bass farming wastewater, and adding a carrier-immobilized compound microbial agent to the wastewater before planting the barley, peas, and herbs. By synergistically adding the carrier-immobilized compound microbial agent and planting specific plants (barley, peas, and herbs) in the water, a highly efficient plant-carrier-immobilized compound microbial agent synergistic purification system is constructed. Through the triple synergistic effect of plants, microorganisms, and the carrier, the system effectively overcomes the problems of low efficiency, incomplete nitrogen and phosphorus removal, and poor system stability inherent in traditional single-plant purification technologies, thereby achieving stable and efficient purification of California bass farming wastewater with high pollution loads.

Provided herein are RNAi delivery compositions and methods for use in targeting organisms found in a plant environment. Features include modification of a chassis microorganism to include exogenous nucleic acids encoding for an siRNA. The compositions and methods provided herein can be used for delivery to a wide variety of crops and for targeting one or more plant-associated pathogens.