68 results about "Plant microbe" patented technology

The invention relates to the technical field of ecological restoration, in particular to a preparation method of novel vegetation concrete greening additive AB bacteria, which comprises the following steps: step (1) screening and activating double bacteria; (2) preparing an inner core structure; (3) loading double bacteria; (4) coating a shell layer; (5) loading a functional matrix; and (6) preparing the vegetation concrete greening additive AB bacteria. According to the method, a multi-layer and function-synergistic microorganism-seed-carrier composite system is constructed and used for surface concrete attachment of the high steep slope, and a plant-microorganism symbiotic system with physical isolation, chemical buffering and nutrition self-sustaining capacities is actively constructed for plant seeds and functional microorganisms in a strongly alkaline macroscopic environment; and finally, the long-acting ecological stability of the high and steep slope is realized.

The invention provides a low-carbon modular artificial wetland system and a carbon sink collaborative purification method thereof, and relates to the technical field of artificial wetlands, the low-carbon modular artificial wetland system comprises a modular box body, and a water inlet layer, a carbon sink layer and a purification layer which are sequentially arranged in the modular box body from top to bottom; the water inlet layer is used for filtering suspended matters through coarse sand; the carbon sequestration layer is used for adsorbing carbon dioxide through biochar and carbon sequestration microorganisms, and meanwhile, the carbon sequestration capacity is enhanced through the plant-microorganism synergistic effect; the purification layer is used for purifying a water body through an iron-based phosphorus removal material and a zeolite denitrification filler. The technical problems of low-carbon material selection, carbon sink potential excavation, renewable energy source coupling and greenhouse gas emission inhibition of an existing constructed wetland system are solved, the technical effects of efficient sewage purification and carbon emission reduction are achieved, and a carbon emission reduction closed loop of the whole life cycle is achieved.

The invention relates to a composite microbial inoculant for soil improvement, the composite microbial inoculant for soil improvement is composed of PGPR (Pseudomonas tianjinensis) and AMF (Amplified Macrophage Fungi), the PGPR is Pseudomonas tianjinensis and Pseudomonas oryzae, and the AMF is rhizophagus clarus. The composite microbial inoculant for soil improvement is characterized in that the PGPR is Pseudomonas tianjinensis and Pseudomonas oryzae, and the AMF is Pseudomonas oryzae, the PGPR is Pseudomonas tianjinensis and Pseudomonas oryzae. According to the invention, the Pseudomonas tianjinensis, Pseudomonas oryzae and AMF are compounded, so that a synergistic effect is exerted, an obvious growth promoting effect on the ryegrass in the saline-alkali soil is achieved, the content of photosynthetic pigments in the ryegrass is increased, the contents of soluble sugar, soluble protein, proline and malondialdehyde in the ryegrass are further increased, and the CAT enzyme activity is improved. In addition, the complex microbial inoculant is combined with ryegrass planting, a plant-microorganism synergistic system is formed, salinized soil is effectively improved, the conductivity and pH of the soil are reduced, the content of nitrogen, phosphorus and potassium in the soil and the content of rapidly available phosphorus and rapidly available potassium are increased, and meanwhile the activity of urease and phosphatase is improved.

Provided herein are compositions and methods for use in challenging pathogenic bacteria on plants. Optional features include modification of a donor bacteria to include exogenous nucleic acids encoding for conjugation machinery and gene modification components, such as guide sequence for use in CRISPR. The compositions and methods provided herein can be used for delivery to a wide variety of crops and for targeting one or more pathogens.

The invention relates to the technical field of heavy metal contaminated soil remediation, in particular to a plant-microorganism-passivator synergistic remediation method for a heavy metal contaminated farmland, which comprises the following steps: step 1, constructing a soil digital twinborn model; step 2, intelligent decision making and verification of a collaborative restoration scheme; 3, soil pretreatment before remediation; step 4, hierarchical collaborative restoration and dynamic regulation and control; 5, performing long-term verification on the repair effect and performing model iteration; physical heat treatment is used as strengthening pretreatment, the occurrence form of heavy metal is changed through high temperature, biomass charcoal is generated in situ, passivation immobilization-microbial strengthening-plant extraction three-stage collaborative operation is carried out according to the time sequence, pollutants are rapidly locked through a passivating agent, rhizosphere immobilization is strengthened through functional microorganisms, plant growth is promoted, and the quality of plants is improved. And the hyperaccumulator finally removes the heavy metals from the soil, so that the unification of short-term control and long-term reduction is realized.

The present disclosure provides non-intergeneric remodeled microbes that are able to fix atmospheric nitrogen and deliver such to plants in a targeted, efficient, and environmentally sustainable manner. The utilization of the taught microbial products will enable farmers to realize more productive and predictable crop yields without the nutrient degradation, leaching, or toxic runoff associated with traditional synthetically derived nitrogen fertilizer, by mitigating or eliminating the need for exogenous nitrogen-containing fertilizers. The remodeled microbes have unique colonization and nitrogen fixation abilities, which enable the microbes to deliver nitrogen to a cereal plant in a spatially targeted (e.g. rhizospheric) and temporally targeted (e.g. during advantageous stages of plant's life cycle) manner. The microbes are able to replace the standard agricultural practice of sidedressing and enable a more environmentally sustainable form of farming. The present disclosure also provides methods of using non-intergeneric remodeled microbes, for example, to fix atmospheric nitrogen by reducing or eliminating the need for exogenous nitrogen-containing fertilizers, to increase yield, and to reduce infield variability in the yield.

The invention provides a method for repairing a coal gangue area without foreign soil by utilizing plant-microorganism combination, and aims to form a self-sustaining repairing system through plant planting and biological characteristics and circulation of root exudate-microorganism metabolism of microorganisms, so that the ecological environment of the coal gangue area is effectively improved, vegetation recovery is promoted, and leaching water pollution is reduced. According to the method, the characteristics of rapid growth, high stress resistance, large biomass, good soil improvement effect and the like of the Juncao are fully utilized, a large amount of litters are produced through multiple times of cutting and laying, then a soil-like matrix is formed, the barren soil structure of the coal gangue area is improved, and conditions are created for subsequent plant community succession; meanwhile, green coverage can be quickly formed by matching with climbing characteristics of climbing plants, visual pollution of a coal gangue area is improved, and the landscape of a mining area is improved. The method provides a new thought and technical means for the treatment of the coal gangue area.

The invention provides a method for strengthening plant microorganism combined remediation of heavy metal contaminated soil, and relates to the technical field of soil remediation. The method for strengthening the plant and microorganism combined remediation of the heavy metal contaminated soil comprises the following steps: a) selecting and planting pioneer plants with tolerance to target heavy metal; b) applying a compound microbial agent containing at least two functional microorganisms, wherein the functional microorganisms comprise plant growth-promoting bacteria and microorganisms forming a symbiotic relationship with plants; c) loading the functional microorganisms on a biochar carrier, and applying the biochar carrier to soil; d) selectively adding an exogenous chelating agent according to the effective state concentration of the heavy metals in the soil; and e) selectively carrying out genetic engineering modification on the functional microorganisms or pioneer plants according to repair requirements. The technology is high in flexibility, optimization combination can be carried out according to different heavy metal types, combined pollution and soil conditions, and the advantages of high efficiency, environmental safety and long-term sustainability of the remediation process are achieved.

A modular ecological floating island with a plant-microbe combination, characterized in that it comprises the following: a floating shell body which is provided with a cavity extending through a thickness direction of the floating shell body; a planting trough that has a tubular structure with an open top and a closed bottom and is removablely arranged within the cavity, where the underside of the planting trough is lower than the lower surface of the floating bowl body; a filter screen that is attached horizontally inside the planting trough and divides the planting trough into an upper planting area and a lower bioreaction area; where aquatic plants are planted in the planting area; wherein the floating shell body is provided on one side with a tenon connection structure, wherein the adjacent floating shell bodies are joined horizontally and vertically by the tenon connection structure.

The present invention proposes a water treatment system that couples anoxic biofilters with artificial wetlands, belonging to the field of water treatment technology. The key technical points are: sewage is collected through a pipe network and flows into a water collection pool, and then enters a regulating / anoxic pool through a lift pump. The solid carbon source in the pool provides a carrier for microorganisms while achieving slow-release supplementation of the carbon source, thereby strengthening the denitrification reaction to remove total nitrogen. The system is then transported to a biofilter and a contact oxidation pool by a lift pump, and COD and ammonia nitrogen are removed through carbon oxidation and nitrification reactions. The effluent enters a high-efficiency clarifier, and after most of the total phosphorus and SS are removed through chemical phosphorus removal and coagulation and sedimentation, the overflow enters an artificial wetland, and is subjected to a comprehensive treatment of plant-microorganism-filler to deeply purify the pollutants and meet the discharge standards. Using the water treatment method of the present application, the effluent water quality can be improved to the Class B emission standard.

The invention belongs to the technical field of riparian zone restoration and treatment, and mainly relates to a plant-microorganism coupling bank protection method suitable for cities in cold regions. The method specifically comprises the following steps: selecting zoysia japonica as revetment vegetation, selecting bacillus subtilis and pseudomonas stutzeri as revetment microorganisms, and constructing a plant-microorganism coupling system to realize natural ecological revetment. The method is a novel ecological bank protection technology integrating multiple functions of river bank reinforcement, water quality purification, soil remediation and the like, the stability of the river bank can be remarkably enhanced, water and soil loss is reduced, water and soil are purified, the ecological environment is continuously improved, and a new thought method is provided for river and lake shoreline remediation; and the method has a good practical engineering application prospect.

The invention discloses a water quality purification system based on a heterogeneous catalytic material and an ecological floating bed. The water quality purification system comprises a floating bed device, a purification device, a fixing device and a water moving trolley, the floating bed device comprises a floating bed carrier and aquatic plants planted on the floating bed carrier; root systems of the aquatic plants extend into the water, and microorganisms are attached to the root systems; the purification device is arranged on the floating bed carrier, is in contact with water flow and comprises a plurality of purification assemblies distributed on the floating bed carrier at intervals, and each purification assembly comprises a purification bag suspended on the floating bed carrier and a heterogeneous catalytic material loaded in the purification bag; the fixing device is arranged on the floating bed carrier and fixes the floating bed carrier on the water surface of a target area; the water moving trolley is connected with the floating bed carrier and drives the floating bed carrier to the water surface of the target area; according to the water quality purification system disclosed by the invention, a material-plant-microorganism three-in-one synergistic purification framework is constructed, and the potential of an ecological floating bed in the aspects of sustainable pollution control and phosphorus resource recovery is remarkably enhanced.

The invention discloses a multi-source composite peptide instant solid beverage and an enzymolysis preparation method thereof, and belongs to the technical field of functional food processing.The beverage is prepared by taking animal, plant, microorganism, insect and algae five-element composite protein sources as raw materials, conducting enzymolysis and then compounding with functional auxiliary materials, a flavoring agent and a stabilizer. Wherein the active peptide fragment is high in proportion and is modified by functional groups. The preparation method comprises the following steps: crushing the multi-element protein, carrying out ultrasonic pretreatment, carrying out enzymolysis by adopting a compound enzyme system containing multiple proteases, carrying out enzyme deactivation and nanofiltration graded purification, and carrying out supercritical COspray drying or freeze drying to obtain the solid beverage. The problems that a traditional peptide beverage is single in protein source, low in biological activity, poor in instant solubility and the like are solved, the obtained product is comprehensive in amino acid composition and has the characteristics of rapid cold water instant dissolution, long-term stability and non-layering, a synergistic effect is formed among functional components, the absorption efficiency and physiological functions such as immune regulation and oxidation resistance are remarkably improved, and the beverage is suitable for large-scale production. Wide industrial application prospects are realized.

The invention provides a submerged plant-microorganism synergistic lake ecological restoration system and application thereof, and belongs to the technical field of water body ecological restoration. The submerged plant-microorganism synergistic lake ecological restoration system comprises a functional complementary symbiotic combination module and a three-dimensional microenvironment enhancement module, the functional complementary symbiotic combination module comprises a submerged plant community, a microbial community, an inducer and a nutritive salt regulating agent; the three-dimensional microenvironment enhancement module comprises a rhizosphere microenvironment optimization unit, a water body microenvironment regulation and control unit and a substrate microenvironment improvement unit. According to the system, by constructing the functional complementary symbiotic combination module and the three-dimensional microenvironment enhancement module, efficient coupling and synergistic interaction of submerged plants and microbial communities are achieved, and lake water quality and ecology can be systematically and comprehensively improved.

This disclosure relates to a method for improving the colonization efficiency of rhizosphere growth-promoting bacteria using nanomaterials and a magnetic interaction system. The method includes: S1, mixing modified iron oxide nanoparticles with a suspension of rhizosphere growth-promoting bacteria and then conducting a first culture to obtain modified rhizosphere growth-promoting bacteria; S2, applying a dispersion of magnetic iron oxide nanoparticles to the roots of a plant for a second culture to obtain modified plants; S3, applying the suspension of the modified rhizosphere growth-promoting bacteria to the roots of the modified plants for a third culture; wherein the modified iron oxide nanoparticles are cationic polymer-modified iron oxide nanoparticles. This method constructs a plant-microorganism magnetic interaction system, improving the colonization ability of rhizosphere growth-promoting bacteria in plant roots, and also enhancing the nitrogen-fixing ability of the rhizosphere growth-promoting bacteria, effectively promoting plant growth.

The invention discloses a seed coating method and an identification method based on a microbial anti-counterfeiting technology. The seed coating method comprises the following steps: S1, selecting 1-3 kinds of specific microbial powder, mixing according to a specific proportion, screening through a net, removing larger particles, and collecting uniform and fine mixed powder; s2, performing corresponding confidential recording on the information of the microorganisms in the powder and the information of the seeds to be coated, and making a confidential recording comparison table; s3, fully mixing the screened microbial powder in the S1 with the seed granulation layer powder according to a certain proportion to form microbial anti-counterfeiting granule layer coating powder; specific plant microorganisms and combinations thereof are inoculated to the seeds in a pelleting and coating mode, and due to factors such as a plant growth regulator secreted by the microorganisms or promotion of nutrient absorption of the seeds, seed germination can be promoted, the seed germination rate can be increased, the stress resistance of the seeds is further improved, and the seed quality is improved.

The invention discloses a method for behavioral growth of plants and microorganisms under catalysis of nitrogen-based nutrition in the crossing field of biotechnology, agriculture and microbial engineering. Nitrogen-based substances of an organism are extracted to prepare a targeted nutrition carrier, and nutrition and environmental parameters are accurately regulated and controlled in combination with physical and chemical means, so that the limitation of a traditional technology is broken through, and low-grade plants and specific microorganisms can grow according to preset patterns (such as human body images). Compared with the traditional technology, the method does not need high-cost external intervention, has the functions of resource circulation, art expression and ecological restoration, and provides an innovative technical path for commemorative art, ecological restoration and personalized gardening development.

The invention discloses a chenopodium ambrosioides seed endophytic fungus Alternaria alstroemia HZ132. The endophytic fungus Alternaria alstroemia HZ132 is preserved in Guangdong Microbial Culture Collection Center with the preservation number of GDMCC No. 66913, and the preservation number of the endophytic fungus Alternaria alstroemia HZ132 in Guangdong Microbial Culture Collection Center is GDMCC No. 66913. The strain has strong capabilities of solubilizing phosphorus, producing indoleacetic acid, producing siderophores and resisting cadmium, is inoculated with HZ132 under cadmium stress, can significantly promote plant growth and enhance cadmium resistance of plants, and has important significance in plant-microorganism combined remediation of heavy metal contaminated soil.

The invention belongs to the technical field of mining area ecological restoration and soil microbial engineering, and particularly relates to a loess sandstorm coal mine area reclaimed soil microbial diversity maintaining method. A'plant-microorganism-soil 'coordinated regulation and control system is designed, a'strip-type crop rotation' planting mode is provided, and microbial communities with specific functions are synchronously inoculated, so that a microbial network structure of the reclaimed soil is reconstructed, a microbial interaction network is constructed, and the complexity and stability of the reconstructed microbial network are evaluated; and dynamically regulating and controlling the reconstructed soil microorganism system according to an evaluation result to stably maintain the biological diversity of the micro soil. The method provided by the invention is suitable for mining area reclamation scenes with strong drought and wind erosion and heavy metal pollution, and has the advantages of wide adaptability, high microbial community construction efficiency, strong stress resistance and disturbance resistance and the like. And a systematized and refined regulation and control path can be provided for microbial diversity recovery of the reclaimed soil in the loess sandstorm coal mine area.

The invention discloses a soil heavy metal remediation method based on indigenous plant-microorganism-charcoal synergy, and belongs to the technical field of soil remediation. The soil heavy metal remediation method comprises the following steps that S1, a heavy metal polluted land parcel is selected, and the variety and content of heavy metal in the land parcel are analyzed; s2, selecting crop rotation plants according to heavy metal pollution conditions; s3, preparing an activated carbon-functional microbial agent; s4, cleaning the heavy metal polluted land parcel, applying an activated carbon-functional microbial agent, and ploughing; s5, sowing seeds of the selected plants, and performing field management, harvesting and crop rotation. A'indigenous plant-functional microorganism-modified charcoal 'synergistic remediation system is constructed, through efficient synergism of targeted plant rotation, acid modified charcoal fixation and compound microorganism activation, the total amount and the effective state content of various heavy metals in soil can be remarkably reduced, meanwhile, the physical and chemical properties of the soil are improved, and the soil remediation effect is improved. And efficient, green and sustainable treatment of the heavy metal contaminated soil is achieved.

The application provides a method for repairing mine wasteland by synergistically activating shield slurry and sludge by plants and microorganisms, injecting ozone into domestic sludge to obtain modified sludge, uniformly mixing the modified sludge, the shield slurry, biological bacterial fertilizer and arbuscular mycorrhizal preparation at a dry weight ratio of (4-5):(1-2):(0.2-0.3):(0.2-0.3) to obtain mixed slurry, performing pressure filtration dewatering treatment on the mixed slurry, and controlling the water content to be 30-50% to obtain mixed dry material, adding tailing sand and pioneer grass seeds to the mixed dry material, irrigating and cultivating at room temperature for 7 days to obtain mixed plant sludge cake, crushing the mixed plant sludge cake to obtain ecological restoration soil, adding ecological curing agent to the ecological restoration soil to obtain cured restoration soil, adding grass seeds to the cured restoration soil to obtain modified restoration soil, adding grass seeds to the ecological restoration soil after laying the ecological restoration soil on the flat section of the mine wasteland, spraying and sowing the modified restoration soil on the slope section of the mine wasteland, repairing the mine wasteland by the ecological restoration soil and the modified restoration soil, and realizing ecological environment restoration succession cycle.

The invention discloses a biomass charcoal material-based modular artificial wetland water quality stabilizing system, which comprises a plurality of independent wetland modules, the independent wetland modules are transversely or longitudinally connected with one another through quick connectors, and each independent wetland module comprises a vertical layered water quality purification system, a self-adaptive hydraulic regulation and control system and an energy self-supply system; the vertical layered water quality purification system comprises a water supply layer, a matrix layer and a plant-microorganism layer, the matrix layer comprises a magnetic biochar layer, a middle slow-release microorganism carrier layer and a bottom permeable conductive concrete layer, and all the layers are integrated with optical fiber sensors and are connected to a central control system; the plurality of bionic hydraulic structures are arranged in the independent wetland modules to form a spirally rising water flow path; through the cooperation of the vertical layered water quality purification system, the self-adaptive hydraulic regulation and control system and the energy self-supply system, the functions of water quality purification and energy utilization are realized, so that the stability of the water quality of the wetland is ensured.

The invention discloses application of adenine in promoting corn growth, and belongs to the technical field of microorganisms and agriculture. The invention finds and verifies that adenine is a key signal molecule for driving the assembly of corn genotype-specific microbiome for the first time, and exogenous addition can directly and efficiently convert a microbial community structure to a beneficial direction; exogenous application of adenine can significantly promote increase of root length, fresh weight and dry weight of corn seedlings and increase starch content, which indicates that the adenine optimizes the physiological status and carbon assimilation efficiency of plants. Adenine is a natural purine substance, is environment-friendly, is different from chemical fertilizers and pesticides, and accords with the development direction of green sustainable agriculture. According to the invention, a new plant-microorganism interaction mechanism is disclosed from a causal chain of'plant signal-microorganism response-phenotypic feedback ', and a brand new theoretical basis and a technical path are provided for crop micro-ecological regulation and control.

The invention discloses a method for purifying micropterus salmoides culture tail water, and belongs to the technical field of aquaculture wastewater treatment. The purification method comprises the following steps: planting barley, hemp peas and vanilla in micropterus salmoides culture tail water, and adding a carrier immobilized complex microbial inoculant into the micropterus salmoides culture tail water before planting the barley, the hemp peas and the vanilla. A high-efficiency plant-carrier immobilized complex microbial inoculant synergistic purification system is constructed by cooperatively putting a carrier immobilized complex microbial inoculant into a water body and planting specific plants (barley, hemp peas and vanilla). Through the triple synergistic effect of the plants, the microorganisms and the carrier, the problems of low efficiency, incomplete nitrogen and phosphorus removal, poor system stability and the like in the traditional single plant purification technology are effectively solved, so that stable and efficient purification of the micropterus salmoides culture tail water with high pollution load is realized.

The present invention relates to the technical field of algae control in landscape lake water bodies, and specifically to a method for controlling algae in landscape water bodies based on a plant-microorganism electrochemical system, which is composed of aquatic plants and a microbial electrochemical system. The aquatic plants include submerged plants (potaeros, foxtail algae, vallisneria, and hornwort) and floating plants (water lilies, water spinach, and water hyacinth). The main body of the microbial electrochemical system is composed of an anode electrode and a cathode electrode. The anode electrode is made of nickel-plated carbon felt, and the cathode electrode is made of a vanadium-based hybrid material. The anode electrode and the cathode electrode are connected by a stainless steel rod, and an anaerobic biofilm is attached to the surface of the anode. The present invention achieves the purpose of controlling algae in urban landscape water bodies by coupling plants and microbial electrochemical systems. At the same time, it can also simultaneously remove pollutants such as nitrogen, phosphorus, and organic matter in water bodies and sediments. It has the characteristics of simple operation, low cost, and excellent treatment effect.

The invention provides a method for repairing a mining wasteland by utilizing plant-microorganism synergistic activation shield slurry and sludge, which comprises the following steps: injecting ozone into domestic sludge to obtain modified sludge, and uniformly mixing the modified sludge, the shield slurry, a biological bacterial fertilizer and an arbuscular mycorrhiza preparation according to a dry weight ratio of (4-5): (1-2): (0.2-0.3): (0.2-0.3) to obtain mixed slurry; carrying out filter pressing dehydration treatment on the mixed slurry, and controlling the water content to be 30-50% to obtain a mixed dried material; adding tailing sand and pioneer grass seeds into the mixed dried material, irrigating and cultivating for 7 days at room temperature to obtain a mixed plant mud cake, and crushing to obtain ecological restoration soil; adding an ecological curing agent into the ecological restoration soil to obtain cured restoration soil, and adding grass seeds into the cured restoration soil to obtain modified restoration soil; after ecological restoration soil is laid on the flat land section of the mine wasteland, grass seeds are added; the modified restoration soil is sprayed and sown on the slope section of the mine wasteland, the mine wasteland is restored through the ecological restoration soil and the modified restoration soil, and the succession cycle of ecological environment restoration is achieved.

The utility model discloses an aquatic plant-microorganism combined bottom mud remediation reactor, which belongs to the technical field of sludge treatment and comprises an ecological cylinder, bottom mud is laid at the bottom of the ecological cylinder and used for planting aquatic plants, overlying water is added into the ecological cylinder, and an adding fixing device is inserted into the top of the bottom mud. The bottom of the adding fixing device is communicated with the interior of the bottom mud through an opening, the top of the adding fixing device penetrates through overlying water to be communicated with the outside, and the adding fixing device is used for adding microbial agents; the ecological cylinder is further provided with a water flow simulation device used for simulating water flow. According to the aquatic plant-microorganism combined in-situ remediation system, an aquatic plant-microorganism combined in-situ remediation scheme with the highest cost performance can be obtained through low economic investment, and a better effect is achieved with low operation cost and maintenance cost.