Method for repairing heavy metal-contaminated soil by combining sludge biochar immobilized bacteria with plants

A technology for phytoremediation and polluted soil, applied in the restoration of polluted soil, etc., can solve the problems of difficult competition of indigenous microorganisms, prone to functional degradation, and difficult survival of microorganisms, so as to promote the extraction of heavy metals, improve the ability of survival and colonization, and improve The effect of plant biomass

Active Publication Date: 2021-02-23
HUNAN ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, artificially added microorganisms with repairing functions have many problems such as difficulty in survival in the environment, low repairing efficiency, difficulty in competing with indigenous microorganisms, and prone to functional degradation.

Method used

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  • Method for repairing heavy metal-contaminated soil by combining sludge biochar immobilized bacteria with plants
  • Method for repairing heavy metal-contaminated soil by combining sludge biochar immobilized bacteria with plants
  • Method for repairing heavy metal-contaminated soil by combining sludge biochar immobilized bacteria with plants

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: A kind of method of sludge biochar immobilization bacteria combined with phytoremediation of heavy metal polluted soil comprises the following steps:

[0030] S1, screen out the black locust that has high resistance level to heavy metal cadmium;

[0031] S2. Screen and isolate Robinia pseudoacacia rhizosphere bacteria resistant to various heavy metals from the repair plants in the mining area. The Robinia pseudoacacia rhizosphere bacteria can be added to the rhizosphere of various plants including Robinia pseudoacacia to play a role;

[0032] S3, screen the bacteria resistant to multiple heavy metals obtained in step S2, and isolate a single bacterial strain capable of dissolving phosphorus, fixing nitrogen, secreting auxin (IAA and ACC) and secreting siderophore;

[0033] S4. Using a pot test, the bacteria obtained in step S3 are subjected to a plant reconnection test, and the bacteria that can promote plant biomass accumulation, root development, and gro...

Embodiment 2

[0036] Embodiment 2: A kind of method of sludge biochar immobilization bacteria combined with phytoremediation of heavy metal polluted soil comprises the following steps:

[0037] S1, screen out the black locust that has high resistance level to heavy metal cadmium;

[0038] S2. Screen and isolate Robinia pseudoacacia rhizosphere bacteria resistant to various heavy metals from the repair plants in the mining area. The Robinia pseudoacacia rhizosphere bacteria can be added to the rhizosphere of various plants including Robinia pseudoacacia to play a role;

[0039] S3, screen the bacteria resistant to multiple heavy metals obtained in step S2, and isolate a single bacterial strain capable of dissolving phosphorus, fixing nitrogen, secreting auxin (IAA and ACC) and secreting siderophore;

[0040] S4. Using a pot test, the bacteria obtained in step S3 are subjected to a plant backgrafting test to obtain bacteria that can promote plant biomass accumulation, root development, and gr...

Embodiment 3

[0043] Embodiment 3: A kind of method of sludge biochar immobilization bacteria combined with phytoremediation of heavy metal polluted soil comprises the following steps:

[0044] S1, screen out the black locust that has high resistance level to heavy metal cadmium;

[0045] S2. Screen and isolate Robinia pseudoacacia rhizosphere bacteria resistant to various heavy metals from the repair plants in the mining area. The Robinia pseudoacacia rhizosphere bacteria can be added to the rhizosphere of various plants including Robinia pseudoacacia to play a role;

[0046] S3, screen the bacteria resistant to multiple heavy metals obtained in step S2, and isolate a single bacterial strain capable of dissolving phosphorus, fixing nitrogen, secreting auxin (IAA and ACC) and secreting siderophore;

[0047] S4. Using a pot test, the bacteria obtained in step S3 are subjected to a plant backgrafting test to obtain bacteria that can promote plant biomass accumulation, root development, and gr...

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Abstract

The invention relates to a method for remediating heavy metal-contaminated soil by combining sludge biochar immobilized bacteria with plants. The method comprises the following steps: screening plants; screening and separating bacteria resistant to various heavy metals; separating out bacteria capable of dissolving phosphorus, fixing nitrogen, secreting plant growth hormone, secreting siderophore,promoting biomass accumulation of plants, root development and growth hormone secretion; loading and fixing the bacteria on sludge biochar; repairing soil; and the like. According to the invention, the sludge biochar is used for immobilizing strains, and microorganisms are protected by the biochar and can be slowly released in soil, so the survival and field planting capacity of the microorganisms in plant bodies and rhizospheres is effectively improved, the biomass of plants is greatly improved, and the heavy metal content of soil is effectively reduced; and meanwhile, root extraction of remediation plants is promoted, and the goal of remediation of heavy metal pollution of the soil by a combination of plant-microorganism-carbon-based material is achieved.

Description

technical field [0001] The invention relates to the field of heavy metal soil remediation, in particular to a method for remediating heavy metal polluted soil with sludge biochar immobilization bacteria combined with plants. Background technique [0002] There are many bioremediation technologies currently applied to heavy metal-contaminated soil in mining areas, among which the method of using plant-microbe joint remediation to achieve synergistic effect is widely recognized. However, at present, artificially added microorganisms with repairing functions have many problems such as difficulty in surviving in the environment, low repairing efficiency, difficulty in competing with indigenous microorganisms, and prone to functional degradation. Contents of the invention [0003] Based on the above-mentioned shortcomings of the above-mentioned prior art, it is necessary to provide a method of sludge biochar immobilization bacteria combined with phytoremediation of heavy metal ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10B09C1/00
CPCB09C1/10B09C1/105
Inventor 张轩李辉黄忠良吴子剑黄兢覃晓莉喻金兰姚雨轩谭梦娇江世林
Owner HUNAN ACAD OF FORESTRY
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