23results about How to "Enhance colonization ability" patented technology

The invention belongs to the field of tumor drugs, and discloses a radioactive hydrogel based on crosslinking of sodium alginate and metal ions and a preparation method and application thereof.The preparation method of the radioactive hydrogel comprises the following steps that lutetium chloride and metal ions are mixed, and a metal ion mixed solution is obtained; doping 177Lu radioactive isotope into the obtained metal ion mixed solution to obtain a metal ion cross-linking agent; and injecting a metal ion cross-linking agent into the sodium alginate solution under a vortex condition, centrifuging, and discarding the supernatant to obtain the radioactive hydrogel. The radioactive hydrogel (177Lu-RH) provided by the invention has good in-tumor colonization capability, can be directly injected into a tumor to realize local radiotherapy, has good radioactive stability, and can be stably retained at a tumor part for more than 120 hours, so that the stability of radioactive labeling in a treatment process is ensured.

The invention provides bifidobacterium bifidum and application thereof, and relates to the technical field of microorganisms. The invention provides Bifidobacterium bifidum NKU FB3-13, which is preserved in the Guangdong Microbial Culture Collection Center, the preservation number of which is GDMCC No: 64747, and the preservation number of which is GDMCC No: 64747. The invention also provides a preparation method of the Bifidobacterium bifidum NKU FB3-13. The strain has good acid resistance and can well adapt to the stomach environment; the strain can effectively reduce the colonization number of helicobacter pylori on gastric epithelium, can greatly reduce the colonization number of helicobacter pylori in animal experiments, relieves inflammation caused by helicobacter pylori infection, and reduces stomach intestinal metaplasia. The preparation method has a wide application prospect in the fields of clinical prevention, treatment and alleviation of diseases related to helicobacter pylori infection, gastrointestinal health-care food and the like.

The invention relates to a culture method of lactic acid bacteria adapting to vaginal environment and strongly adhering to vaginal epithelial cells, which comprises the following steps: inoculating a lactic acid bacteria seed solution obtained by culture into a liquid culture medium, standing and culturing until the OD600 value of the culture solution is 0.3-0.6, and adding a preparation for continuous culture, and obtaining the lactic acid bacteria strongly adhering to the vaginal epithelial cells after the fermentation is finished. The preparation comprises L-arabinose. The invention further relates to application of the L-arabinose in preparation of a preparation for improving the vaginal epithelial cell adhesion capacity of the lactic acid bacteria, and by adding the related preparation in the specific growth stage of the lactic acid bacteria, rapid proliferation of thalli and stable formation of surface hydrophobic protein can be guaranteed; therefore, the adhesion and planting capabilities of the cultured lactic acid bacteria are further improved.

This invention relates to the field of microbial technology. Specifically, it relates to a highly colonizing and aroma-producing brewing yeast, CX51, and its application in red wine production. This brewing yeast, CX51, is deposited at the China General Microbiological Culture Collection Center (CGMCC) on November 19, 2025, with accession number CGMCC No. 39063. In multi-strain mixed fermentation, CX51 effectively suppresses the proliferation of native brewing yeasts and maintains a significant competitive advantage over commercial brewing yeasts, exhibiting outstanding colonization ability. Fermentation of 'Marselle' wine solely with CX51 significantly increases the content of ethanol esters and acetate esters in the wine, enhancing floral and fruity aromas and optimizing the aroma composition and sensory quality. Brewing yeast CX51 has broad prospects for industrial application in the production of specialty red wines.

The application discloses a kind of bio-organic fertilizer and preparation method thereof, belong to organic fertilizer technical field.The bio-organic fertilizer includes by weight parts: plant source organic matter matrix 62-75 parts, carbonized rice husk 10-20 parts, compound functional bacterial agent 0.5-3 parts, mineral conditioning component 0.5-2 parts.Plant source organic matter matrix is composed of rice bran, corn meal, soybean meal, soybean oil;Compound functional bacterial agent is compounded by indigenous functional bacterial flora and artificial intensive bacterial flora according to mass ratio 1:(1-2);Preparation whole process uses normal temperature powder process, avoids high temperature inactivation bacterial flora.The whole plant source formula of the application eliminates heavy metal and antibiotic pollution of livestock and poultry manure source, and the porous carrier of carbonized rice husk cooperates with normal temperature process to greatly improve the survival rate of bacterial flora, and the double bacterial system is adapted to local soil, and is colonized for a long time, which can realize multiple functions such as organic matter supplement, acid-base two-way adjustment, granular structure repair, soil-borne disease prevention and control.

The invention relates to the technical field of probiotics, in particular to intestinal tract source pediococcus acidilactici of Yanqu county in Tibet and fat reducing application thereof. The pediococcus acidilactici disclosed by the invention is preserved in the China General Microbiological Culture Collection Center on April 29, 2025, and the preservation number is CGMCC NO.34406; the strain has the characteristics of acid resistance, cholate resistance and stress resistance, and has high survival rate and planting efficiency; the strain is free of hemolysis, free of toxic factors and high in safety; the strain can secrete short-chain fatty acid and antibacterial peptide, inhibit pathogenic bacteria and promote intestinal barrier repair; short-chain fatty acid generated by metabolism of most of carbon water by the strain can be directly absorbed by intestinal epithelial cells, and can be used as an energy substance to replace partial glucose demand, so that the metabolism energy consumption is reduced, the energy conversion rate of feed is improved, the feed conversion is promoted, and the effects of reducing fat and weight and improving the non-alcoholic fatty liver disease are achieved.

A probiotic microsphere with in-situ proliferation, its preparation method and application. The microsphere is prepared by a two-step Pickering double emulsion method, encapsulating probiotics and panax notoginseng saponins, and then mixed with sodium alginate and cross-linked by calcium ions. The structure significantly improves the survival rate of probiotics in simulated gastric juice, intestinal juice, heat treatment and antibiotic environment, and maintains high viable cell count during long-term storage. Co-encapsulated PNS and double emulsion stabilizer natural polysaccharide-protein nanocomposite can promote the proliferation of probiotics in-situ, increase their activity. In addition, the microsphere can effectively promote the colonization and proliferation of probiotics in the intestinal tract, enhance the oral bioavailability of bioactive ingredients, and is suitable for functional food, feed, biological medicine field, especially as 3D printing special medical food for dysphagia population and prevention and treatment of intestinal inflammatory diseases.

The invention belongs to the technical field of crop control, and particularly relates to saline-alkaline tolerant bacillus velezensis capable of colonizing trichosanthes kirilowii maxim and application of the bacillus velezensis. The bacillus velezensis is bacillus velezensis MH24, the preservation number of the bacillus velezensis MH24 is CCTCC (China Center For Type Culture Collection) NO: M20221680, and the bacillus velezensis MH24 can be used for preventing and controlling diseases such as trichosanthes kirilowii maxim anthracnose, bacterial angular leaf spot, virus diseases, root rot and root-knot nematode. And the composition has no cross resistance with existing bactericides and insecticides. The strain has saline-alkaline tolerance and can be used for prevention and control and yield increase of soil-borne diseases of trichosanthes kirilowii maxim in salinized soil. Compared with the existing chemical synthetic pesticide for prevention and treatment, the compound is from the natural environment, has the advantages of high efficiency, low toxicity, no environmental pollution, low possibility of generating drug resistance and the like, and has a wide application prospect.

The invention belongs to the technical field of microorganisms, and relates to a streptococcus salivarius subsp. Thermophilus Ss-PG-10 strain for relieving hyperuricemia and a preparation method of the streptococcus salivarius subsp. Thermophilus Ss-PG-10 strain. The strain provided by the invention is streptococcus salivarius subsp. Thermophilus Ss-PG-10, the classification name of the strain is Streptococcus salivarius subsp. Thermophilus, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No. 31867. The streptococcus salivarius subsp. Thermophilus Ss-PG-10 is separated from pickled Chinese cabbages, has a function of relieving hyperuricemia, has a certain antibacterial effect on pathogenic bacteria such as staphylococcus aureus, bacillus subtilis and escherichia coli, and has relatively strong acid resistance, cholate resistance and artificial simulated gastrointestinal fluid resistance.

The present invention relates to the field of microorganisms, and in particular to Lactobacillus amylovora SKLAN202401ZF and applications thereof. Lactobacillus amylovorus ) SKLAN202401ZF, with a deposit number of CGMCC No.30022. This strain can produce the anti-inflammatory metabolite chrysin in the intestines of animals, and has excellent antibacterial and immunomodulatory functions. By adding this strain to piglet feed, the intestinal health of piglets can be significantly improved, the incidence of diarrhea can be reduced, and the feed conversion rate and growth rate can be increased. The application of this strain not only helps to improve the efficiency of pig farming, but also can replace antibiotics, making it suitable for antibiotic-free farming.

The present application belongs to the field of microbial technology, and particularly relates to a synbiotic preparation of lactobacillus helveticus, a preparation method and application thereof. The synbiotic preparation of lactobacillus helveticus provided by the present application is a microcapsule preparation prepared by embedding lactobacillus helveticus as probiotics and sialylated human milk oligosaccharide as prebiotics in turn by using sodium alginate and chitosan. The lactobacillus helveticus is lactobacillus helveticus HL88. The lactobacillus helveticus HL88 used in the present application has better lysis activity on sialylated human milk oligosaccharide, and can effectively improve the utilization efficiency of sialylated human milk oligosaccharide. Further, the lactobacillus helveticus and sialylated human milk oligosaccharide are complexly embedded in the present application, which can play a synergistic metabolic role, thereby effectively promoting the brain development process. Therefore, the present application can provide a treatment idea for improving brain development retardation, and can provide a technical basis for developing probiotic drugs for promoting brain development.

The present application relates to the technical field of feed engineering and microbial application, and discloses a probiotic chicken feed and a preparation method thereof.The feed comprises corn flour, soybean meal, wheat bran, a synergistically modified probiotic carrier, natural plant enzyme and various probiotics, wherein the probiotics comprise Lactobacillus delbrueckii subsp.Bulgaricus, Bacillus subtilis, yeast and rice koji.The synergistically modified probiotic carrier is formed into a limited inclusion and ion crosslinking structure by β-cyclodextrin and chitosan in the presence of calcium lactate, and is used to coat and modify the probiotics and natural plant enzyme.Through the above structure design, the stability of the probiotics and enzyme during processing and storage can be effectively improved, and the survival ability in the gastrointestinal environment and the intestinal colonization effect can be enhanced, so as to improve the feed utilization rate and improve the growth performance of chickens.The present application has simple process, is suitable for large-scale production, and has good application prospect.

This invention relates to the field of biomedical technology, disclosing a targeted probiotic nanocapsule, its preparation method, and its application. The method involves using a photosensitizing agent ICG and polysaccharides to covalently couple probiotics to prepare probiotic nanocapsules. The core material of the microcapsule is primarily probiotics, and the microcapsule wall material is carboxymethyl dextran and the photosensitizing agent ICG. These microcapsules are easy to prepare, exhibit good sustained-release and controlled-release properties, and the materials are non-toxic, biocompatible, and biodegradable. This invention improves the wall material of the probiotic microcapsules and designs the preparation method and overall process accordingly. By utilizing polysaccharides and photosensitizing agents to form the capsule wall, the resulting nano-probiotics are acid-resistant, bile salt-resistant, digestive enzyme-resistant, and heat-resistant, resulting in improved probiotic stability and enhanced intestinal colonization.

The invention discloses a method for preparing a functional biological flora in a targeted biological flora and host original flora coupling mode. The method comprises the following steps: screening a single strain with a function of degrading pesticides, antibiotics or heavy metals; purifying and amplifying the single strain, and compounding according to a ratio to obtain a compound flora; carrying out coupling symbiotic culture on the compound flora and a host original flora to obtain a coupled flora; carrying out thermal shock and gradient domestication on the coupled flora, and screening stress-tolerant sporulation strains; carrying out targeted delivery treatment on the stress-tolerant strain by utilizing microcapsule embedding, lipidosome or nanoparticles; the invention relates to the technical field of agriculture, animal husbandry and environmental restoration, and aims to realize the synergistic symbiosis of the flora and the host environment and improve the colonization capability and action effect of the microbial inoculum. Multiple functions of degrading pesticide residues, antibiotics and heavy metals are realized; high temperature resistance, acid and alkali resistance and adaptability to complex environments; the targeting property is strong, and the action efficiency is high.

This invention discloses a method for preparing functional compost, belonging to the field of agricultural waste resource utilization and grassland ecological restoration technology. The key technical points include: mixing fresh sheep manure, wheat straw, and corn straw at a specific wet weight ratio and adding superphosphate to obtain a basic mixture; inoculating this mixture with a compound microbial fermentation agent composed of *Bacillus steatophilus*, *Bacillus subtilis*, and *Bacillus mucilaginosus* at a specific viable count ratio to obtain an inoculum; subjecting the inoculum to aerobic composting fermentation and implementing dynamic turning control based on dual temperature thresholds to maintain the compost within a specific high-temperature range for a sufficient number of days; and finally, aging the material until its seed germination index reaches a specific range to obtain functional compost. The functional compost prepared by this method is mainly used for restoring degraded grazing grasslands.

The present application relates to the field of agricultural informatization and intelligent management, in particular to a full-process management system for selenium-rich bio-organic fertilizer research and development and demonstration and popularization, comprising: a raw material digital filing module for executing a sliding average filtering algorithm to clean data and generating a digital raw material fingerprint feature vector; a market demand reverse analysis module for extracting and calculating an environmental demand constraint matrix representing differentiated market demand based on preset element flow rules; a research and production intelligent decision-making module for starting an evolutionary solution model, mapping the raw material fingerprint to the environmental constraint matrix, and outputting a dynamic production management instruction sheet; a production execution supervision module for distributing instructions to terminals and real-time monitoring of process execution data; and a full-life-cycle closed-loop optimization module for obtaining feedback data to calculate deviations and generating a correction factor to update model parameters; the present application solves the contradiction between the heterogeneity of non-standard biomass raw materials and the precise nutritional requirements of functional agricultural products.

The application belongs to the technical field of microorganisms, and discloses a salt and alkali tolerant squirrel mammalian coccus and application thereof. Mammaliicoccus sciuri ​ STM10, which is a gram-positive coccus, has been preserved in the China General Microbiological Culture Collection Center with a preservation number of CGMCC No.38450. The strain has salt and alkali tolerance, can play the promoting functions of nitrogen fixation, phosphorus dissolution and resolution, iron carrier production and protease production, and can be prepared into microbial fertilizer. Application of the microbial fertilizer can effectively relieve salt and alkali stress, improve the seed germination and growth ability of crops, improve the physical and chemical properties of saline-alkali soil, optimize the ion environment, promote the nutrient absorption of crops, and provide support for agricultural utilization of saline-alkali land.

This invention relates to the field of wastewater treatment technology, and more particularly to a method for enhancing anaerobic ammonia oxidation (AAO) denitrification using iron-loaded polyurethane porous materials. The method includes the following steps: Anaerobic ammonia oxidation sludge, iron-loaded polyurethane porous materials, and wastewater containing ammonia nitrogen and nitrite nitrogen are thoroughly mixed in an anaerobic reactor and reacted under anaerobic conditions at a temperature of 31-35°C. The iron-loaded polyurethane porous materials are prepared as follows: polyurethane sponge is selected as a carrier, cut and cleaned, and then immersed and stirred in a mixed iron salt solution for a period of time. The pH of the mixed solution is adjusted to alkaline, resulting in the formation of a red precipitate. The iron-loaded polyurethane porous materials are then hydrothermally synthesized. The mixed iron salt solution includes ferric chloride and ferric nitrate. The carrier material prepared by this invention, when applied to an anaerobic ammonia oxidation system, can effectively shorten the process start-up time, promote the enrichment and biofilm formation of anaerobic ammonia oxidizing bacteria, and achieve a high total nitrogen removal rate.

The invention provides a preparation method and application of a biomass charcoal-based soil conditioner. The method comprises the following steps: carrying out ball milling on biochar and titanium gypsum to prepare titanium gypsum-biochar (TBC), and immobilizing sphingomonas by using TBC to obtain the TBC immobilized sphingomonas. The biomass charcoal-based soil conditioner prepared by the invention has the advantages of large specific surface area, high porosity, high calcium ion content and the like, and when the biomass charcoal-based soil conditioner is applied to the problems caused by continuous cropping of cowpeas, the phenolic acid content in soil can be reduced, the stress resistance of the cowpeas can be effectively improved, the physicochemical properties of the soil are improved, and the quality of the cowpeas is improved. Therefore, the adverse effect of continuous cropping obstacles on the growth of cowpeas is relieved.

The invention discloses a compound microbial functional fertilizer as well as a preparation method and application thereof, and belongs to the technical field of microbial fertilizers. The functional fertilizer is prepared by taking six functional strains including clostridium butyricum (CCTCC NO: M 2022151), bacillus coagulans (CGMCC NO.32216), kluyveromyces marxianus (CGMCC NO.24455) and the like as cores, matching with auxiliary materials including soy protein concentrate and the like and performing a two-stage compound fermentation process. Experiments show that the functional fertilizer can significantly increase the content of medium trace elements such as quick-acting phosphorus, potassium, calcium, magnesium, iron and zinc in soil, and effectively repair the degraded soil structure; when the fertilizer is used for wheat planting, the acre yield, the wheat protein content and the wet gluten content of wheat can be remarkably improved, and the crop quality is greatly improved. The soil remediation agent has the dual effects of soil remediation and crop yield increase and quality improvement, and a new technical scheme is provided for sustainable development of green agriculture.

The present invention discloses a composition having the function of relieving ulcerative colitis and its application, and belongs to the technical field of microorganisms and microorganism addition. The present invention shows through in vivo and in vitro experiments that the composition can significantly improve the weight loss and disease activity index increase of mice with ulcerative colitis, reverse the colon atrophy caused by colitis, protect the integrity of colon tissue structure, reduce the level of inflammation in the intestine, and has a good relieving effect on colitis. At the same time, the composition can promote the abundance of targeted promotion of Lactobacillus, enhance the colonization of Lactobacillus johnsonii in the intestine, and is conducive to its prebiotic effect. The composition is used to prepare a composition and fermented food for the preparation of drugs for relieving ulcerative colitis and related diseases, and has a very broad application prospect.

The present invention relates to a Chinese medicine probiotic composition for improving oral bacteria, and a preparation method and application thereof, and belongs to the technical field of probiotic application. The Chinese medicine probiotic composition of the present invention is formed by fermenting a Chinese medicine extract with a probiotic composition, and the Chinese medicine extract is extracted from the Chinese medicine composition; the probiotic composition is composed of the following raw materials: Lactobacillus rhamnosus, Lactobacillus reuteri, Lactobacillus bulgaricus, Bacillus subtilis, Bifidobacterium, Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus salivarius; the Chinese medicine composition is composed of the following raw materials: Jerusalem artichoke, Artemisia argyi, cinnamon bark, Evergreen, tea tree leaf, Magnolia officinalis, corn silk, clove, Ginkgo biloba, licorice, loquat leaf and Polygonatum odoratum. The Chinese medicine probiotic composition of the present invention converts the Chinese medicine extract into a small molecule substance that is easily absorbed by fermentation, and then improves the oral bacteria group through the generated effective components, maintains oral health, and can effectively prevent and treat problems such as dental diseases and bad breath.

The invention discloses lactobacillus reuteri RD003, a microbial agent containing the lactobacillus reuteri RD003 and application of the lactobacillus reuteri RD003 and belongs to the technical field of microorganisms. The invention aims to provide lactobacillus reuteri which can be added into animal feed to improve the immunity of sika deer and the flora abundance in rumen fluid. The lactobacillus reuteri RD003 is preserved in the China Center for Type Culture Collection, the preservation date is June 9, 2022, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M 2022847. The invention provides a theoretical basis for the use of sika deer feed or feed additives.