439results about How to "Enhance colonization ability" patented technology

The present invention discloses a fruit and vegetable probiotic tablet and a preparation method thereof. The fruit and vegetable probiotic tablet uses probiotic powders such as lactobacillus plantarum powder as the main raw material, and the preparation method scientifically mixes modified dietary fibers, fruit and vegetable powder, oligosaccharides, plant extracts, protein powder, tea leaf extracts and traditional Chinese medicine extracts and etc., thus improves the content of soluble celluloses which are of real significance for probiotic flora, enhances the physiological activity of celluloses, thereby increases the species of intestinal probiotic flora as well as significantly enhances the colonization ability and time of endogenous and exogenous probiotics in the human intestinal tracts, effectively inhibits the growth and reproduction of harmful intestinal bacteria, especially gram-negative bacteria, and fully regulates the composition of the intestinal probiotic flora. The prepared fruit and vegetable probiotic tablet has a high biological activity, a long human intestinal colonization time, and a significant weight loss effect, and is suitable for a wide range of people.

The invention discloses a production method of a pig feed additive containing traditional Chinese medicine and active probiotics, which comprises the steps of: respectively conducting single-spawn strain quantity expanding culture to the parent strains of active probiotics for pigs; mixing equiponderant first-order seed liquid of all kinds of active probiotics for pigs, inoculating the liquid into seed tank culture mediums for multi-spawn mixed culture and then inoculating the liquid into traditional Chinese medicine liquid culture mediums for primary traditional Chinese medicine fermentation; and mixing multi-spawn mixed traditional Chinese medicine fermentation liquid with corn cob meal with the same volume to obtain solid fermented seeds and then inoculating the seeds into traditional Chinese medicine solid culture mediums for secondary traditional Chinese medicine fermentation. By implementing the embodiment of the invention, the strain activity of the active probiotics in the pigfeed additive and the colonization performance in intestinal canals can be greatly improved, the produced pig feed additive is rich in traditional Chinese medicine compositions, the effect of active compositions which take a pharmacological effect in the traditional Chinese medicine compositions can be fully exerted, the body strengthening and disease curing performance and the growth promoting performance of the pig feed additive are improved and therefore the use of antibiotics can be fully avoided.

The invention discloses a bdellovibrio bacteriovorus for preventing and curing rice bacterial diseases and application thereof. The invention can obtain bdellovibrio bacteriovorus BDFM05 by separationand ultraviolet mutation. The bdellovibrio bacteriovorus BDFM05 is combined with oligosaccharide to obtain bdellovibrio bacteriovorus for preventing and curing crop bacterial diseases. The inventionapplies bdellovibrio bacteriovorus BDFM05 to prevent and cure crop bacterial diseases for the first time. The bdellovibrio bacteriovorus prepared by the method has better rice bacterial diseases preventing and curing effect than common Saisentong, has no toxicity or pollution, is safe to natural enemies of crop diseases and pests, produces no pathogenic bacteria resistance, has no toxic or side effects to people and animals, improves the quality and safety of agricultural products and conforms to the requirements of people on healthy and green food and sustainable-development for agriculturalproduction.

The invention relates to a biological preparation, in particular to a Trichodrema spp. microbial agent and a preparation method thereof. The method comprises the following steps: the dried and crushed 8-9 edible fungus chaff according to parts by weight is mixed with 2-4 auxiliary material according to parts by weight, the acidity-alkalinity pH of the mixed raw material is adjusted to 5.0-6.5, then the Trichodrema spp. suspension with 5-10 percent of mass ratio with the mixed raw material is inoculated to the mixed raw material and cultured for 7-10 days at the temperature of 8-30 DEG C, and after the Trichodrema spp. is generated, the well cultured material is dried and crushed to obtain Trichodrema spp. microbial agent. The invention takes the treated edible fungus chaff as the raw material to produce the Trichodrema spp. microbial agent, fully utilizes the rest nutritious substance in the edible fungus chaff as well as the characteristics of loose and permeability of the fungus chaff, promotes the value in use of the fungus chaff of the edible fungus, and simultaneously reduces the production cost of the Trichodrema spp. microbial agent, the produced Trichodrema spp. is mainly chlamydospore, the multiplication capacity and the colonizing capacity of the Trichodrema spp. in the microbial agent are enhanced, the capability of resisting the unfavorable environmental conditions is also enhanced and the survival time of the Trichodrema spp. is prolonged.

The invention discloses a nutritional health food with the function of improving gastrointestinal function and a preparation method thereof. The nutritional health food can be used for regulating the intestinal flora to improve the gastrointestinal function from the aspects of increasing the probiotics type and quantity, increasing the probiotics nutrient substance, inhibiting the growth of harmful bacteria and enhancing the immunity of human body. According to the preparation method, probiotics powder such as lactobacillus plantarum is used as the main raw material, and edible fungi extracts and modified dietary fibers are scientifically compounded; saussurea involucrata culture extracts which are capable of remarkably enhancing the immunity of human body, resisting inflammatory and easing pain, and have the functions of oxidation resistance, radiation resistance and fatigue resistance are used, so that while the intestinal probiotics flora types is increased, the fix planting capacity of the endogenous probiotics and exogenous probiotics in the intestinal tracts of human body is remarkably enhanced, and fix planting time of the endogenous probiotics and exogenous probiotics in the intestinal tracts of human body is prolonged remarkably, the growth and reproduction of the harmful bacteria, particularly the Gram negative bacteria, in the intestinal tracts are effectively inhibited, the forming of the probiotic floras in the intestinal tracts is fully regulated and the nutritional health food which is good in probiotic property, capable of remarkably enhancing the immunity of human body and has the effect of improving the gastrointestinal function is prepared.

The invention discloses a health food capable of improving intestinal floras and a preparation method of the health food. The intestinal floras are adjusted on a plurality of aspects of increasing the probiotics variety and quantity, adding probiotic nutrient substances, inhibiting the harmful bacterial growth and increasing the human immunity, so that the gastrointestinal functions are improved. With probiotic powder such as lactobacillus plantarum as a main raw material, an edible mushroom extract is scientifically compounded, and dietary fibers are modified, so that the human immunity can be significantly improved; a saussurea involucrate culture extract has the efficacies of resisting inflammation, relieving pain, and resisting oxidation, radiation and fatigue; the variety of the intestinal beneficial bacteria floras is increased; the field planting capacity of endogenous and exogenous probiotics in human intestinal tracts is significantly enhanced and the field planting time of endogenous and exogenous probiotics in human intestinal tracts is significantly prolonged; the growth and the reproduction of intestinal harmful bacteria, especially gram negative bacteria, are effectively inhibited; the components of the intestinal beneficial bacteria floras are fully adjusted; and the health food which is high in probiotic property, and is capable of significantly improving the human immunity and improving the intestinal floras is prepared.

The invention relates to a preparation method for probiotic yoghurt. The preparation method is characterized in that: the preparation method comprises the following steps: evenly mixing fresh milk, sucrose and a stabilizer; carrying out homogenizing and sterilization; and then adding a ferment prepared from probiotic powder with high activity to prepare the probiotic yoghurt through mixing and fermentation. The prepared yoghurt contains probiotics with a high survival rate, and has higher nutritive value.

The invention discloses a probiotic-containing health food and a preparation method thereof, wherein intestinal florae are adjusted in many aspects of increasing the types and quantity of probiotics, increasing the nutrient substances of the probiotics, inhibiting the growth of harmful fungi, enhancing human immunity and the like, so as to improve gastrointestinal functions. Probiotic powders of lactobacillus plantarum and the like are taken as main raw materials, and edible fungi extract, modified dietary fibres, a saussurea involucrata culture extract capable of remarkably enhancing the human immunity, resisting inflammation and easing pain and having anti-oxidization, anti-radiation, anti-fatigue functions and the like are scientifically compounded, and then the planting capacity and the planting time of endogenous and exogenous probiotics in human intestinal tracts are remarkably improved and prolonged, the growth and reproduction of intestinal harmful fungi, especially, Gram-negative bacterium, are effectively inhibited, and the composition of intestinal probiotic florae is adequately regulated while the types of the intestinal probiotic florae are increased; therefore, a health food with high probiotic property, capable of remarkably enhancing human immunity and containing probiotics is prepared.

The invention discloses weight-losing probiotic tablets and a preparation method thereof. According to the weight-losing probiotic tablets and the preparation method thereof, probiotic powder such as lactobacillus plantarum is taken as a main raw material, and modified dietary fibres, fruit and vegetable powder, oligosaccharides, plant extract, protein powder, tea leaf extract, Chinese herb extracts and the like are scientifically compounded, thus increasing the content of soluble celluloses with real significance in probiotic groups, and enhancing the physiological activity of the celluloses, and then remarkably improving the planting capacity and the planting time of endogenous and exogenous probiotics in human intestinal tracts, effectively suppressing the growth and the reproduction of intestinal harmful bacteria, in particular, Gram negative bacteria, and adequately regulating the composition of the intestinal probiotic groups while increasing the types of the intestinal probiotic groups; the prepared weight-losing probiotic tablets are high in bioactivity, long in planting time in the human intestinal tracts, remarkable in weight-losing effect, and wide in the range of appropriate crowds.

The present invention is recompounded microbe germicide for preventing and controlling banded sclerotial blight and green smut of rice. The recompounded microbe germicide is recompounded through thefermentation culture of Bacillus subtilis and recompounding the fermented liquid with certain content of Bacillus subtilis and Jinggangmeisu. It is used in preventing and controlling banded sclerotial blight and green smut of rice in field via utilizing the complementary superiority of Bacillus subtilis and Jinggangmeisu. It has high functions of preventing and controlling banded sclerotial blight and green smut of rice, less influence on rice quality, stable germicidal effect and long preservation period.

The invention provides an antagonistic bacterium for controlling radix rehmannia root rot. The antagonistic bacterium is named as pseudomonas aeruginosa LK-12 (the Preservation No.: CGMCC 10966) after authentication. The pseudomonas aeruginosa LK-12 disclosed by the invention is obtained by screening from continuous cropping rhizosphere soil of radix rehmannia root through an agar disk diffusion method. Fusarium oxysporum and aspergillus flavus pathogenic bacteria separated from lesion parts of the radix rehmannia root have a high antagonistic effect; in the meanwhile, fusarium oxysporum, fusarium moniliforme, talaromyces sp. and other pathogenic bacteria separated from lesion parts of radix pseudostellariae also have a remarkable restriction effect and can be used for effectively controlling root rot diseases of medicinal plants comprising the radix rehmannia root to provide a strain for overcoming or relieving replant diseases of the medicinal plants. Therefore, the antagonistic bacterium is a potential biological control strain which is good in biological control effect, free of pollution as well as safe and ecological, and is wide in development and application prospects.

The present invention discloses lactobacillus rhamnosus, named lactobacillus rhamnosus HCS01-003. The strain is preserved on August 14, 2018 in the "China General Microbiological Culture Collection Center", and the preservation number is CGMCC No.16257. The invention also discloses an application of the strain used as an additive lactobacillus rhamnosus lyophilized powder which has strong anti-digestive tract anti-environment ability and good bacteriostatic property in preparing foods, health foods or pharmaceutical compositions. The invention further discloses an application of the strain used as a food additive lactobacillus rhamnosus lyophilized powder which has strong anti-digestive tract anti-environment ability and good bacteriostatic property in preparing infant foods.

The invention discloses a saline-alkali soil modified microbial inoculum and a preparation method thereof. The microbial inoculum comprises, by weight, 10-30 parts of bacillus megatherium HB bacteriapowder, 35-65 parts of phosphorus tailings powder, 12-18 parts of phosphogypsum, 8-25 parts of sugar refinery wastes, 6-26 parts of seaweed residue and 1-8 parts of mannitol. Components of the saline-alkali soil modified microbial inoculum have synergistic interactions with each other to enhance effects, if any one of the components lacks, expected effects cannot be reached, and only when the saline-alkali soil modified microbial inoculum with whole formulas is adopted and 30-80 kg of the microbial inoculum is used per mu, can the effects of increasing fertilizer utilization rate and crop yields, and improving quality and saline-alkali lands be achieved.

The invention provides new burkholderia cenocepacia and application thereof. The bacterium is identified as new burkholderia cenocepacia and is named as Burkholderia cenocepacia CJ-P8 with the collection number of CGMCC NO.12119. The new Burkholderia cenocepacia CJ-P8 disclosed by the invention is obtained by screening from radix pseudostellariae rhizosphere soil by adopting a plate confrontation process, respectively has a strong antagonistic effect on radix pseudostellariae soil-borne pathogens such as fusarium oxysporum and fusarium moniliforme which are obtained through separation in an early stage, and has no pathogenicity on other crops in a radix pseudostellariae producing area. The invention also provides an optimal medium for growth of the antagonistic bacterium, an optimal formula is 1/4 LB+1/80 MS (does not contain ferric salt)+0.1wt% of brown sugar, and fermented liquid has an obvious antagonistic effect. The antagonistic strain provided by the invention can be used for effectively preventing and treating soil-borne diseases of radix pseudostellariae and is pollution-free, ecological and safe, thereby having good application prospects.

The present invention belongs to the technical field of agricultural microbe, and specifically relates to a microbial capsule preparation taking poly-gamma-glutamic acid as a wall material, a preparation method thereof, and applications thereof in preparing microbial pesticides and microbial fertilizers. The microbial microcapsules taking the poly-gamma-glutamic acid as the wall material have good mechanical strength and flexibility, can be applied in microbial embedding medium for good sustained-release ability, can prevent microbial cells from injuries of ultraviolet rays, temperature, acid or base, ionic strength and heavy metal ions, can prevent microbial cells from damages in a production process, and have the advantages of effectively extending the shelf life of the preparation and the like. The microbial microcapsule preparation of the present invention can prepare different microbial microcapsule preparations according to different microbial species, and can effectively control capsule wall thickness and diameter of the microcapsules in the preparation process. The microcapsules of the invention have an embedding rate of more than 90% for microbial cells, and can realize industrial mass production.

The invention relates to bacillus megaterium, a polypeptide agricultural microbial agent thereof as well as a preparation method thereof and applicationof the polypeptide agricultural microbial agent.The bacillus megaterium takes distillers' grains, soya bean meal and straw powder, which are rich in protein content, as main raw materials, and are mixed with corn flour, wheat bran, inorganic saltsand a few carbon source to reasonably compound and steam; high-yield protease bacillus subtilis is firstly utilized, the bacillus megaterium is utilized for decomposing different proteins in raw materials under a special fermentation condition to prepare the agricultural microbial agent which takes active peptides, amino acid, polysaccharides and the like as efficient ingredients; the microbial agent contains a great number of living spores, 17 free amino acids, a plurality of active peptides, and polysaccharides, and nutrient content is rich, the activity of thallus is high, and shelf life reaches two years or more; and according to the field experiment, the good effects of improving quality and increasing the yield are achieved.

The invention belongs to the technical field of formulas of edible-medicinal foods and new resource foods, and particularly provides a medical formula food, a specific medical formula food or a non-specific medical formula food for patients suffering insomnia. The formula is prepared according to the requirements of the <General Rules for Formula Foods for Special Medical Purposes>, and combines the physical characteristics of patients suffering insomnia. The formula food provided by the invention is prepared by reasonably mixing various 'edible-medicinal' traditional Chinese medicines according to the quintessence theory of traditional Chinese doctors, and is prepared by mixing essences which are extracted from various edible-medicinal traditional Chinese medicines by adopting a prehydrolysis SBE technique, various micro-encapsulated probiotics, short peptides extracted from biological enzymolysis, prebiotics, an amino acid, carbohydrate, grease with a healthy keeping function, various vitamins and mineral substances. The formula food can be used as a single nutrient source meeting the nutrient demands of patients suffering insomnia, and has the efficacies of soothing nerves, assisting sleep, benefiting qi, nourishing blood, nourishing yin to lessen fire, calming livers to lessen fire, nourishing hearts and livers, and preventing and curing insomnia.

The invention relates to two ralstonia-solanacearum-resistant endophytic bacillus velezensis strains and application thereof. By separation, screening and purification in roots of healthy tobacco plants in a ralstonia solanacearum occurrence area of the Enshi Prefecture, two biocontrol bacillus endophyticus strains R-3 and R-9 are obtained, have evident resistance effects on ralstonia solanacearumand are both capable of generating high-temperature-resistant ralstonia-solanacearum-resistant substances. By gyrB gene sequence identification and phylogenetic tree construction, results show that the R-3 and R-9 are both bacillus velezensis; by potted plant root irrigation experimental verification, the bacilli R-3 and R-9 have great colonization performance in tobacco roots and can enter stemsthrough root transmission; by field experimental verification, bacillus velezensis R-3 and R-9 are effective in ralstonia solanacearum prevention and treatment, remarkably superior to chemical agentkasugamycin and free of pollution. The biocontrol endophyte research field is further enriched by researching on the bacilli R-3 and R-9, and a guiding significance to ralstonia solanacearum prevention and treatment is achieved.

The invention discloses a method for preparing a grass carp feed additive containing traditional Chinese medicine active probiotics, comprising the following steps: respectively carrying out amplification culture of the strain number of a single bacterial specie on original strains of active probiotics for grass carps; mixing primary seed liquors of active probiotics for grass carps with same weight, inoculating the mixture in a seed tank medium to carry out multiple-strain mixed culture, then inoculating in a traditional Chinese medicine liquid medium to carry out a primary traditional Chinese medicine fermentation; and mixing the multiple-strain mixed traditional Chinese medicine fermentation liquor with isometric cob powder to obtain solid fermentation seeds, and then inoculating in a traditional Chinese medicine solid medium to carry out a secondary traditional Chinese medicine fermentation. According to the invention, the strain activity and colonization performance in the intestinal tracts of active probiotics in the grass carp feed additive are improved greatly, the prepared grass carp feed additive is rich in traditional Chinese medicine components, the active components with pharmacological effects in the traditional Chinese medicine components fully play the role, thus the performances of strengthening the body, treating diseases and promoting growth of the grass carp feed additive are improved, and the grass carp feed additive can effectively treat and prevent four diseases of grass carps, and furthermore completely displace antibiotics.

The invention discloses a pest-repelling biological organic fertilizer for dragon fruit trees, and belongs to the technical field of preparation of fertilizers. The pest-repelling biological organic fertilizer for dragon fruit trees comprises a tobacco fertilizer, Nerium indicum leaves, onion, Crotalaria mucronata, tea leaves, barnyard manure, bagasse, plant ash, a cake fertilizer, animal manure, household garbage, ammonium dihydrogen phosphate, Aspergillus oryzae, yeast, Bacillus subtilis, mycorrhizal fungi, Azotobacter vinelandii, photosynthetic bacteria and Paecilomyces lilacinus. The tobacco fertilizer, Nerium indicum leaves, onion, Crotalaria mucronata and tea leaves having a pest repelling function are added to the pest-repelling biological organic fertilizer, so the stress resistance of the dragon fruit trees is improved, grubs, mole crickets, termites, ground nematodes and other dragon fruit tree plantation common pests are effectively dispelled, the use of pesticides is reduced, and the quality of dragon fruits is improved.