24 results about "Vaginal epithelial cell" patented technology

The invention discloses application of tannic acid combined triazole medicines in preparation of medicines for treating diseases caused by fungi, and belongs to the technical field of antifungal. A series of drug sensitive tests show that tannic acid lower than 3 [mu] g / ml has no influence on the activity of human vaginal epithelial cells, and after the tannic acid is combined with fluconazole, the tannic acid can significantly inhibit the growth activity of drug-resistant candida (including drug-resistant candida albicans and drug-resistant candida glabrata). In view of the natural property, wide source and low toxicity of tannic acid, the invention provides a new antifungal treatment strategy, and the tannic acid has important clinical application value for treating infection (such as vaginal candidiasis) caused by drug-resistant fungi, especially under the condition that the existing antifungal drugs are poor in effect. Therefore, the tannic acid combined triazole medicine can be used for preparing the medicine for treating diseases caused by fungi.

The invention discloses combined lactobacillus salivarius CCFM1332 with a multi-target regulation effect and application of the combined lactobacillus salivarius CCFM1332 in periodontitis, and belongs to the technical field of microorganisms. The invention discloses a strain of combined lactobacillus salivarius CCFM1332, and the combined lactobacillus salivarius not only has a vaginal immune regulation effect, but also has an effect of relieving periodontitis, which is specifically embodied in that: the combined lactobacillus salivarius can promote vaginal epithelial cells VK2 / E6E7 to secrete antibacterial peptides; the periodontal probing and diagnosing depth of the periodontitis rats can be reduced; the number of pathogenic bacteria in the oral cavity of the periodontitis rat is reduced; the levels of inflammatory factors IL-1beta, IL-6 and IL-17A in serum are reduced; alveolar bone loss of rats suffering from periodontitis is reduced, and the alveolar bone absorption condition of the rats is effectively relieved.

The application discloses a human vagina micro-ecological in-vitro model and a construction method and application thereof, and relates to the technical field of medicines.The application discloses a human vagina micro-ecological in-vitro model and a construction method and application thereof.Firstly, a mixed cell group of the vaginal epithelium containing a complete cell lineage and capable of long-term subculture is successfully established, a technical bottleneck that primary vaginal epithelial cells are difficult to expand is solved, and ideal seed cells are provided for model construction.Secondly, a highly-bionic vaginal mucosa model having a tight connection structure, rich layers and an orderly arrangement is constructed, and the physiological barrier and immune regulation functions of the vaginal mucosa are truly simulated.Finally, by introducing lactobacillus symbiosis and confirming the response to estrogen, a multi-element in-vitro research model integrating the anatomical structure of the vagina, resident flora, local immunity and endocrine regulation is constructed, and a reliable technical platform is provided for in-depth exploration of the mechanism of vaginal micro-ecological disorder and efficient screening of related drugs.

The invention discloses a composition of type III collagen and beta-glucan and application of the composition in repairing vaginal mucosa injury, and belongs to the technical field of biological medicine, and the composition comprises the following effective components: type III collagen with the concentration of 0.3-1.8 mg / mL and beta-glucan with the mass concentration of 0.1-0.8%. The collagen III provides structural support required by tissue repair, the beta-glucan improves inflammation microenvironment, regulates immune response and jointly promotes proliferation and migration of damaged vaginal epithelial cells, the cell proliferation rate and the scratch healing rate are far higher than those of a group using any component independently, and rapid repair of mucosal lesions can be achieved.

The present invention relates to a method for culturing lactic acid bacteria that adapt to the vaginal environment and strongly adhere to vaginal epithelial cells. The method comprises inoculating the cultured lactic acid bacteria seed solution into a liquid culture medium, and culturing the culture medium statically until the OD value of the culture medium reaches 0. 600 When the value is 0.3-0.6, the preparation is added and culture is continued, and after completion, the lactic acid bacteria that strongly adhere to vaginal epithelial cells are obtained; the preparation includes L-arabinose. The present invention also relates to the use of L-arabinose in preparing a preparation for improving the adhesion of lactic acid bacteria to vaginal epithelial cells. By adding the relevant preparation during a specific growth stage of lactic acid bacteria, it is beneficial to ensure rapid bacterial proliferation and stable formation of surface hydrophobic proteins, thereby further improving the adhesion and colonization ability of the cultured lactic acid bacteria.

The present application relates to the technical field of microorganisms, in particular to a lactobacillus crispatus Lcr5-6 and application thereof, the lactobacillus crispatus Lcr5-6 is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.38486;The adhesion rate of the lactobacillus crispatus Lcr5-6 to Hela cells is as high as 61.11%, which is significantly higher than that of the control strain lactobacillus delbrueckii DM8909 (12.13%), has excellent vaginal epithelial cell adhesion and colonization ability, and can be long-term colonization in the vagina to play a probiotic role.

The invention discloses a sheep oestrus monitoring system based on cell image morphology recognition, and the system is characterized in that the system comprises a sample collection module (100) which obtains a sheep vagina epithelial cell sample and carries out the dyeing of the cell sample; the microscopic imaging module (200) performs optical amplification on the dyed cell sample to generate a cell morphology image; the image processing module (300) extracts cell edge contours and quantifies morphological irregular features; and the intelligent analysis module (400) judges the estrus state of the sheep by comprehensively analyzing the nuclear off-center proportion and the form irregularity index. The sheep oestrus monitoring system based on cell image morphological recognition has the advantages that high-sensitivity capture of biological markers in the early oestrus stage is achieved by fusing dual morphological criteria of cell nucleus spatial displacement analysis and contour geometric distortion detection, and the timeliness and accuracy of reproduction regulation and control are remarkably improved.

The invention provides a construction method of a human vaginal epithelium organ and application of the human vaginal epithelium organ in construction of a chlamydia trachomatis infection model, and belongs to the technical field of biomedicine. The invention provides a liquid culture medium which takes a human cervical organ culture medium as a basic culture medium and further comprises Wnt3a. Vaginal epithelial cells are inoculated into the liquid culture medium for culture, and human vaginal epithelial organs (VEOs) can be obtained. The liquid culture medium provided by the invention can support long-term amplification of vaginal epithelial cells for more than 6 months. Moreover, the vaginal epithelial cells obtained by amplification can still maintain the multiplication capacity after being subjected to long-term freeze-thaw cycle, and show excellent stability.

The application discloses a recombinant humanized collagen composition for resisting HPV infection and a preparation method thereof. The composition comprises the following components in percentage by mass: 30-45% of a recombinant humanized collagen fusion protein, 30-40% of probiotics and 20-35% of glycogen. The recombinant humanized collagen fusion protein comprises, from N-terminal to C-terminal, a recombinant humanized collagen fragment and a human fibronectin fragment connected in sequence. The recombinant humanized collagen fragment and the human fibronectin fragment are connected by a linker. The recombinant humanized collagen fusion protein in the composition can promote the adhesion and colonization of the probiotic composition to the vaginal epithelial cells, rapidly improve the microbial environment of the vagina and effectively enhance the resistance of the vagina to harmful bacteria and viruses.

The invention discloses a sperm-friendly medical lubricant and a preparation method thereof. The lubricant comprises the following components: a water-based matrix, an active component, phosphate, a humectant, an osmotic pressure regulator and water, the active ingredients comprise small molecule peptidoglycan and alkaline polypeptide. The alkaline polypeptide is alkaline protein, can mildly improve the pH environment of the vagina and is beneficial to maintaining the activity of sperms, and the small molecule peptidoglycan has a good adhesion effect on vaginal epithelial cells, can be adhered to the vaginal epithelial cells and prevents the alkaline polypeptide from stimulating the vaginal inner wall; the small molecule peptidoglycan also can promote local immune regulation, enhance secretion of anti-inflammatory factors and immune response of macrophages, and improve the anti-infection ability of vagina; the composition of the two active substances can maintain sperm activity and protect and resist infection on the vagina at the same time, and is a lubricant which is friendly to the vagina and sperms.

The present invention addresses the problem of providing a novel therapeutic agent that is effective against diseases involving stratified squamous epithelial cells such as dry eye syndrome. The present invention is an agent for promoting normal differentiation / maturation of stratified squamous epithelial cells, which comprises a secretion of mesenchymal stem cells. It is preferable that the secretions not include animal serum, that the mesenchymal stem cells be adipose-derived mesenchymal stem cells, umbilical cord-derived mesenchymal stem cells, or bone marrow-derived mesenchymal stem cells, and that the stratified squamous epithelial cells be at least one selected from the group consisting of corneal epithelial cells, conjunctival epithelial cells, epidermal keratinocytes, oral epithelial cells, epiglottic epithelial cells, esophageal epithelial cells, vaginal epithelial cells, vocal fold epithelial cells, nasal epithelial cells, and nasal vestibular epithelial cells.

The invention discloses a condom added with sophocarpidine hyaluronic acid lubricating liquid and a preparation method of the condom, and belongs to the technical field of condom production. The core of the method is that after matrine is dissolved, the matrine is added into hyaluronic acid lubricating liquid for the condoms, the hyaluronic acid lubricating liquid containing the matrine is prepared through stirring and mixing, the concentration of the matrine in the hyaluronic acid lubricating liquid is 7.7-25.0 mg / g, when the condoms are subjected to inner packaging, the matrine-containing lubricating agent is added into the condoms, and when the condoms are subjected to inner packaging, the matrine-containing lubricating agent is added into the condoms. And preparing the condom added with the sophocarpidine-containing hyaluronic acid lubricating liquid. According to the condom, inflammation generated after vaginal epithelial cells are stimulated by bacterial structural components is improved through matrine, and the common pathogenic bacteria such as staphylococcus aureus, escherichia coli, enterococcus faecalis and streptococcus agalactiae of vaginitis are inhibited in the using process; and the up-regulation level of NF-kB phosphorylation generated after lipopolysaccharide and peptidoglycan stimulate vaginal epithelial cells is relieved.

This invention belongs to the field of biomedicine and discloses the application of HMOX1 inhibitors in the preparation of drugs for treating senile vaginitis. This invention is the first to discover that the content of the metabolite MESV1 (Guggulsterone) is significantly elevated in the vaginal secretions of patients with senile vaginitis. MESV1 induces ferroptosis in vaginal epithelial cells by upregulating HMOX1 protein expression, thereby driving the disease. Based on this, this invention proposes a novel therapeutic strategy targeting HMOX1. The inhibitors include shRNAs targeting HMOX1 (such as SEQ ID NO: 1-3), which can be prepared into vaginal gels, creams, and other dosage forms. Simultaneously, MESV1 can also be used to prepare auxiliary diagnostic kits. This invention provides a novel target and precise treatment approach for non-hormonal treatment of senile vaginitis.

This application discloses an in vitro reconstructed vaginal mucosa model, its construction method, and its application. The construction method includes an improved culture medium: HVU medium is added to immortalized vaginal epithelial cell line VK2 / E6E7, which is then seeded into Transwell chambers and cultured. The HVU medium on the surface of the culture in the Transwell chambers is aspirated, and the Transwell chambers are raised to the air-liquid interface for air-liquid surface culture. The culture medium is then replaced with HVA medium to obtain the vaginal mucosa model. This method improves the stability of cells during in vitro culture and constructs an in vitro reconstructed vaginal mucosa model with a tissue structure highly similar to human vaginal mucosa (possessing a complete stratified squamous epithelial structure and normal intercellular connections), thereby enhancing the model's physiological functional simulation.

A controlled release, antimicrobial gel for the treatment of vaginal infections, comprises a muco-adhesive gel matrix containing one or more anti-infective agents bound to said matrix and effective for the treatment of vaginal infections and capable of pH dependent delivery of said one or more anti-infective agents as a single application to the vaginal cavity over a period of 3 days, while the muco-adhesive gel matrix remains bound to the vaginal epithelia. The preferred anti-infective agents are thymol and carvacrol in combination. The antimicrobial gel is biocidal against biofilms formed by the causative agents of bacterial vaginosis, trichomoniasis and candidiasis. The antimicrobial gel is effective for achieving sustained delivery of the anti-infective agents to the vaginal cavity at a therapeutically efficacious dose from a single application of the gel for the treatment and prevention, and prevention of recurrence, of bacterial vaginosis, trichomoniasis and candidiasis.

The invention relates to lactobacillus reuteri LR35 beneficial to female genital tract health and having the function of improving gastritis and application of the lactobacillus reuteri LR35. The LR35 strain has excellent acid resistance, acid production capacity, self-agglutination capacity and vaginal epithelial cell adhesion capacity; common vaginal pathogenic bacteria can be antagonized; adhesion and reproduction of pathogenic bacteria can be prevented, and a competitive repulsive effect is achieved; the composition also has an excellent effect of inhibiting inflammatory factors; the symptoms of a mixed vaginitis mouse model can be improved. Meanwhile, the strain also has an antagonistic effect on the main pathogenic bacterium helicobacter pylori causing gastritis, and can form a copolymer with the helicobacter pylori to discharge the helicobacter pylori out of a body; and the traditional Chinese medicine composition also has an inhibiting effect on inflammatory factors secreted by human gastric adenocarcinoma cells, namely has a good potential of improving inflammatory response caused by helicobacter pylori.

The invention belongs to the technical field of biological medicine, and particularly discloses an AIE molecular material and application of the AIE molecular material in preparation of a medicine for treating candida albicans infectious vaginitis. The AIE molecule prepared by the invention can specifically bind and kill candida albicans in an aggregation state, pathogenic bacteria can be completely removed within 30 minutes under 100 mu M, and lactobacillus and vaginal epithelial cells are not damaged. The action mechanism comprises the steps of inducing release of fungal reactive oxygen species (ROS), destroying a cell structure, and remarkably reducing inflammatory factors (IL-6 and IL-1beta) and reducing hypha and neutrophil infiltration through animal experiment verification. And after treatment, the diversity of vaginal microbiota and abundance of lactobacillus are remarkably improved, and the recurrence risk is reduced. The molecule has the functions of fluorescence imaging diagnosis and treatment, is simple and convenient to synthesize, stable and efficient, provides an accurate and safe novel scheme for VVC treatment, and has remarkable clinical application potential.

The invention discloses lactobacillus gasseri WT002 which is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on February 18, 2025, and the preservation number of the lactobacillus gasseri WT002 is CGMCC No.33552. The lactobacillus gasseri WT002 is named as Lactobacillus gasseri WT002. The lactobacillus gasseri WT002 can inhibit the growth of various vaginal pathogenic bacteria, has strong vaginal epithelial cell and cervical cancer cell adhesion ability, can be used for preventing and / or relieving and / or treating genital tract diseases, and further can be used for preparing medicines, hygienic products and the like.

The invention discloses lactobacillus crispatus WT001 which is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on February 18, 2025, and the preservation number of the lactobacillus crispatus WT001 is CGMCC No.33551. The lactobacillus crispatus WT001 has the advantages that the lactobacillus crispatus WT001 is preserved in the China General Microbiological Culture Collection Center on February 18, 2025; the lactobacillus crispatus WT001 can inhibit the growth of various vaginal pathogenic bacteria, has strong vaginal epithelial cell and cervical cancer cell adhesion ability, can be used for preventing and / or relieving and / or treating genital tract diseases, and further can be used for preparing medicines, hygienic products and the like.

The application discloses a lactobacillus crispatus for relieving various vaginitis based on host immune regulation, and belongs to the technical field of microorganisms. The application provides a lactobacillus crispatus CCFM1298, which is preserved in the Guangdong Microbial Culture Collection Center on December 9, 2022, and has a preservation number of GDMCC No: 62275. The lactobacillus crispatus CCFM1298 targets the vaginal immune system, down-regulates pro-inflammatory factors IL-1beta and TNF-alpha, and up-regulates the expression of anti-inflammatory factor IL-10, is suitable for relieving various vaginal inflammations and pathological improvement, and significantly reduces the shedding of vaginal epithelial cells. The lactobacillus crispatus is derived from the vagina of a healthy female, is a probiotic strain suitable for human vaginal application, helps to relieve and improve various vaginal infections of females, and has a good application prospect.

The invention discloses anti-aging, moisturizing and antibacterial vaginal gel, and belongs to the field of daily necessities for health. The gel is composed of a solution A, a solution B and a solution C, wherein the solution A contains 15-25 parts of glycerol, 10-15 parts of sodium hyaluronate and 1-5 parts of xanthan gum; the solution B is prepared from 0.5 to 2 parts of hydroxyethyl cellulose and 1 to 2 parts of carboxymethyl chitosan; the solution C contains 0.5-3 parts of beta-nicotinamide mononucleotide, 0.5-1.5 parts of an antibacterial agent and 0.1-0.8 part of a transdermal absorption enhancer, and further contains coenzyme Q10 and a centella asiatica extract or a lactobacillus fermentation product on the basis. The antibacterial rate of the vaginal gel to pathogenic bacteria is greater than or equal to 99.5%, lactobacilli are not interfered, vaginal epithelial cell collagen can be improved by 35% or above, the mitochondrial activity can be improved by 40%, and the vaginal gel is suitable for preventing infection, delaying tissue aging and maintaining micro-ecological balance and can also be combined with clotrimazole to enhance the antifungal curative effect.

The application discloses a kind of based on cell image morphological identification's sheep estrus monitoring system, including its features in, including sample acquisition module (100) obtains sheep vaginal epithelial cell sample and carries out dyeing treatment to cell sample;Microscopic imaging module (200) carries out optical amplification to the cell sample after dyeing and generates cell morphology image;Image processing module (300) extracts cell edge contour and quantifies morphological irregular feature;Intelligent analysis module (400) is determined by the comprehensive analysis to nuclear deviation center proportion and morphological irregular index, judges sheep estrus state.The application has beneficial effect for proposing a kind of based on cell image morphological identification's sheep estrus monitoring system, by fusing the double morphological criterion of nuclear space displacement analysis and contour geometric distortion detection, realizes the high sensitivity capture to early estrus biological marker, significantly improves the timeliness and precision of reproduction control.

This invention discloses a strain of *Lactobacillus johnsonii* and its metabiotic that simultaneously improves vaginal mucosal damage and inhibits vaginal inflammation, belonging to the field of microbial technology. The *Lactobacillus johnsonii* strain of this invention was isolated from vaginal secretions of healthy women. It has the effects of improving vaginal mucosal damage and inhibiting vaginal inflammation, specifically manifested in: promoting the migration and repair of vaginal epithelial cells; reducing the content of soluble cadherin E in the vaginal mucosa of mice and increasing the expression of vaginal tight junction protein OCLN; reducing the shedding of vaginal mucosal epithelial cells; reducing the secretion of MPO and IL-1β in mice with vaginitis; and (6) improving the pathological characteristics of the vagina in mice. Therefore, this *Lactobacillus johnsonii* has great application potential in products that improve vaginal mucosal damage and inhibit vaginal inflammation.

The invention discloses lactobacillus reuteri for promoting host beta defensin and immune globulin secretion and relieving vaginal mucosa damage through oral administration and external application and a metagen thereof, and belongs to the technical field of microorganisms. The lactobacillus reuteri is separated from vaginal secretion of healthy women, has the effects of improving vaginal mucosa injury and inhibiting vagina inflammation, and is specifically reflected in that secretion of a vaginal epithelial cell host defense peptide HBD2 is promoted; the secretion of a vaginal epithelial cell host polymeric immunoglobulin receptor pIgR is promoted; the secretion of animal vaginal tissue host defense peptide mBD3 is improved; the production of secretory immunoglobulin sIgA is promoted; the generation of animal serum immune globulin IgG is promoted, so that the systemic immunity is improved; the pathological characterization of the vagina of the mouse is improved. Therefore, the lactobacillus reuteri has a huge application prospect in products for enhancing the anti-infection barrier of the host and improving the local immunity and systemic immunity of the host.