The invention relates to the field of traditional Chinese medicines, in particular to a medicine composition for treating vaginitis, which comprises the following components in percentage by weight: 0.1-20.0 percent of blue lotus essential oil, 0.1-20.0 percent of borneol essence, 0.5-5.0 percent of vitamin E, 0.5-15.0 percent of arginine and 0.5-1.0 percent of nitroglycerin or pentaerythritol tetranitrate. The invention also provides a gel of the medicine composition and a preparation method thereof. The medicine composition and the gel thereof can improve the blood circulation around vagina and prevent premature aging of epithelial cells of the vagina, and have no side effect and potential risk on human bodies after long-time use. In the invention, antibiosis experiments show that the medicine composition can inhibit the growth of various pathogenic bacteria, acute skin irritancy tests show that no toxic reaction is found, the microbiological indices of the medicine composition reach the standard, and no pathogenic bacteria exists, thereby the invention has a wide application prospect.