78 results about "Collagen Type III" patented technology

The invention provides a medical-grade recombinant three-type collagen and application thereof. The recombinant humanized three-type collagen has certain cell proliferation promoting capacity and is free of cytotoxicity. The medical-grade recombinant three-type collagen provided by the invention not only has the capability of promoting cell proliferation, is free of cytotoxicity and high in cell adhesion activity, but also has a three-helix structure characterized by the collagen and is extremely high in stability, the purity can reach about 85% after the collagen is induced for 120H in a 100L fermentation tank, the difficulty of a downstream purification process is greatly reduced, and the production cost is reduced. The effect in the aspect of cell activity is obvious.

The invention provides recombinant III-type collagen and application thereof, and relates to the technical field of genetic engineering. According to the invention, the yield of the collagen is improved by optimizing the nucleotide sequence of the target gene and optimizing the culture conditions of pichia pastoris, so that the collagen is efficiently expressed in engineering bacteria. The recombinant engineering bacterium shows strong potential in the aspect of improving the yield of protein with high economic value, and is suitable for large-scale industrial production. The recombinant III-type collagen is high in expression quantity, simple in preparation process and suitable for preparation of biological materials for medical cosmetology, wound repair and the like, and has a wide application prospect.

This invention discloses recombinant type III collagen, its preparation method, and its applications. The amino acid sequence of the recombinant type III collagen is shown in SEQ ID NO: 9. The recombinant type III collagen integrates the RGDV highly active motif and the EKEK hydrophilic stable domain with the core structure of type III collagen to achieve an integrated design of "high activity and long-lasting effect". The in vitro cell migration and collagen secretion effects are improved by more than 50% compared with wild type. Through anti-enzyme cleavage site modification and hydrophilic modification, the in vivo residence half-life is significantly prolonged to meet the long-term repair needs of the dermis.

The invention belongs to the technical field of bioengineering, and particularly relates to a kluyveromyces marxianus engineering strain for recombinant expression of hydroxylated human III-type collagen and application of the kluyveromyces marxianus engineering strain. The Kluyveromyces marxianus engineering strain of the human type III collagen is obtained by carrying out recombinant expression construction on proline hydroxylase and a human type III collagen gene in the Kluyveromyces marxianus; according to the invention, two truncation designs and codon optimization are carried out on the existing natural human III type collagen amino acid sequence to obtain the human III type collagen C3S1 and C3S2, and the recombinant secretory expression in the kluyveromyces marxianus engineering strain is realized. Like hydroxylation modification of the human III type collagen, the human III type collagen C3S1 and the human III type collagen C3S2 which are subjected to recombinant expression have proline hydroxylation characteristics. The recombinant human III-type collagen is prepared by using the kluyveromyces marxianus engineering strain, the method is simple, the production efficiency is high, and large-scale production is easy.

The invention discloses a composition of type III collagen and beta-glucan and application of the composition in repairing vaginal mucosa injury, and belongs to the technical field of biological medicine, and the composition comprises the following effective components: type III collagen with the concentration of 0.3-1.8 mg / mL and beta-glucan with the mass concentration of 0.1-0.8%. The collagen III provides structural support required by tissue repair, the beta-glucan improves inflammation microenvironment, regulates immune response and jointly promotes proliferation and migration of damaged vaginal epithelial cells, the cell proliferation rate and the scratch healing rate are far higher than those of a group using any component independently, and rapid repair of mucosal lesions can be achieved.

The invention discloses recombinant human III-type collagen and application thereof, and belongs to the technical field of biochemical engineering. The method comprises the following steps: according to human III-type collagen, obtaining a target amino acid sequence through splitting, and integrating the target amino acid sequence into an expression vector to obtain plasmids; transforming the plasmid into a host, and screening positive clones to obtain an expression strain; and culturing the expression strain, and separating and purifying to obtain the high-yield and high-purity human III-type collagen. The obtained recombinant human III-type collagen has a collagen triple helix region characteristic sequence (Gly-X-Y) n, has good performances of promoting cell migration and adhesion and preventing light damage, and can be widely applied to the fields of medicines, biological materials, health care products, cosmetics and the like.

The application discloses recombinant collagen type III, a hydrogel, a preparation method and application, and belongs to the technical field of medical materials. The protein has an amino acid sequence as shown in SEQ ID No. 2 or 3, and has excellent biocompatibility and cell activity. The hydrogel based on the protein is formed by mixing a solution of the protein with a solution containing oxidized sodium alginate and hydroxypropyl methyl cellulose, adjusting pH, sequentially introducing an EDC / NHS crosslinking system and calcium ions, and standing. The process constructs a stable double-network structure of EDC / NHS catalyzed covalent crosslinking network and OSA-Ca 2+ ion crosslinking network synergistic effect. The obtained hydrogel has high mechanical strength, excellent toughness, long-lasting wet tissue adhesion and suitable swelling performance, can effectively adapt to the complex stress changes of dynamic wounds, and provides an active matrix for cells, and promotes tissue repair.

This invention belongs to the field of biomedical technology, specifically relating to a delivery system for type III collagen mRNA and its applications. The nucleic acid delivery system of this invention includes a delivery carrier and a delivered nucleic acid, wherein the nucleic acid is collagen mRNA, and the delivery carrier includes one or more of modified amino acids, growth factors, and polymeric materials. Through intradermal injection, the delivery system of this invention can be administered to the dermal layer of the skin and enter relevant cells, utilizing endogenous cellular proteins to achieve mRNA transfection, forming natural human type III collagen. This invention avoids the immunogenicity risks and structural inactivation problems associated with exogenous collagen injections, and can efficiently and safely replenish non-regenerative type III collagen in tissues. It can be used for various indications such as treating skin aging, promoting wound healing, reducing scar formation, and improving vascular elasticity.

The invention belongs to the technical field of protein engineering, and discloses a humanized collagen XVII type polypeptide as well as a preparation method and application thereof. The amino acid sequence of the humanized collagen XVII type polypeptide is as shown in SEQ ID NO.1, and the polynucleotide sequence for coding the humanized collagen XVII type polypeptide is as shown in SEQ ID NO.2. The invention also provides application of the polypeptide in preparation of a protein product with functions of preventing hair loss and growing hair and / or repairing, resisting wrinkles and tightening skin. The application proves that the humanized collagen XVII type polypeptide can promote proliferation of hair papilla cells, keratinocytes and fibroblasts and promote up-regulation of expression of beta-Catenin signal channel protein closely related to hair growth in the hair papilla cells; the up-regulation expression of fibroblast type III collagen, type IV collagen, type VII collagen, fibronectin and elastin is promoted, so that the polypeptide is proved to have the effects of preventing hair loss and growing hair and / or repairing, resisting wrinkles and tightening skin.

The invention relates to an mRNA (messenger Ribonucleic Acid) molecule and application thereof in preparation of beauty and skin care products, and particularly, the mRNA molecule comprises a nucleic acid sequence for coding type III collagen. After the mRNA provided by the invention is applied to the skin, the skin cells can generate collagen, and the aged skin is normalized by increasing the collagen content in the skin tissue, so that the effect of repairing the skin is achieved.

The invention relates to the technical field of gene engineering, and discloses a recombinant human III-type collagen and a preparation method and application thereof.The amino acid sequence of the recombinant human III-type collagen comprises the amino acid sequence shown in SEQ ID NO.1. The amino acid sequence of the recombinant human III-type collagen has the advantages of being high in hydrophilicity, uniform in charge distribution and the like; due to the characteristic, the transdermal absorption efficiency of the product is greatly improved, so that the product can penetrate into the bottom layer of the skin more deeply, and a better effect is exerted. Meanwhile, due to the 100% humanized amino acid sequence design, the high affinity of the product and the human skin is ensured, and the risk of allergic reaction is reduced to the maximum extent.

The invention provides a composition for preventing or treating radioactive skin injury and application of the composition. The composition comprises (1) recombinant III type collagen; (2) component (a): at least one hyaluronic acid or a salt thereof with a molecular weight of 1000-2500k Da; (3) a component (b): at least one hyaluronic acid or a salt thereof with a molecular weight of 200-500 k Da; the component (a), the component (b) and the component (c) form a network structure, so that an excellent external environment can be provided for the collagen, the recombinant collagen can promote cell proliferation and migration, and the component (a), the component (b) and the component (c) have a mutual synergistic effect.

The invention discloses a water-based skin care product containing type IV collagen and a preparation method of the water-based skin care product. The water-based skin care product comprises the recombinant collagen skin care composition and a water-based matrix. The recombinant collagen skin care composition comprises a recombinant IV type collagen, a GLP tripeptide, a PGD tripeptide, a recombinant III type collagen, a recombinant I type collagen and a recombinant human-like collagen according to a specific ratio. According to the invention, the recombinant collagen composition is creatively applied to a water-based skin care product to maintain the collagen of the whole skin layer, so that the effect of comprehensively promoting skin barrier repair is achieved.

The invention provides an oral preparation for improving anemia after gastric resection operation and a preparation method thereof, and relates to the technical field of biological medicines. The oral preparation is prepared from the following raw materials: vitamin B12, internal factors, chickpea protein, III type collagen peptide, a pH regulator, an antioxidant and a dispersing agent. The vitamin B12 and the internal factors are taken as active ingredients, the utilization degree of the vitamin B12 is remarkably improved through the specific proportion of the vitamin B12 and the internal factors, and the prepared reagent meets the requirement that the reagent is not released in a gastric acid environment and then is slowly released in the intestinal tract, and has a remarkable effect on the anemia problem caused by deficiency of the vitamin B12 after the gastric resection operation; and the stability of the medicine is high.

The invention discloses a collagen maintenance liquid dressing with an anti-aging function as well as a preparation method and application of the collagen maintenance liquid dressing, and belongs to the technical field of cosmetics. Comprising the following components in parts by volume: 40-60 parts of distilled water, 20-30 parts of skin care product base oil or olive oil, 10-15 parts of recombinant collagen III, 3-8 parts of a pH regulator, 1-5 parts of an antioxidant and 0.5-2 parts of a plant preservative, wherein the pH regulator is a combination of lactic acid and tartaric acid, and the tartaric acid is selected according to the difference of skin types: malic acid for dry skin, citric acid for oily skin and a malic acid and citric acid compound for neutral or mixed skin. The invention aims to realize full dissolution and effective embedding of the components through batch charging and differential process parameters, and improve the stability and efficacy of the product. A nanoscale crystal structure is constructed, and the permeability and the slow-release effect of active ingredients are enhanced. Special maintenance liquid for different skin types is developed, and functional indexes such as moisturizing, water locking and antioxidation are optimized.

The application discloses a liquid for repairing oral mucosa injury and belongs to the technical field of mucosa repair. The liquid comprises seabuckthorn seed oil extract, Periplaneta americana active polypeptide, recombinant human collagen type III, polyoxyethylene 40 hydrogenated castor oil, poloxamer, propylene glycol, glycerol, dipotassium glycyrrhizinate, sucralose, a cooling agent, nipagin methyl ester, nipagin propyl ester, essence, and purified water. The liquid prepared by the application has a smooth and soft taste, a sweet and pleasant flavor, a fresh and clean breath after spraying / flushing, good product stability, no mucosa irritation, and can quickly repair damaged mucosa, and the preparation method is simple, so that the liquid can be used for industrial large-scale production.

1. The name of the design product: packaging kit (medical recombinant humanized type III collagen dressing). 2. The use of the design product: for product packaging. 3. The design points of the design product: in the pattern. 4. The picture or photo that best indicates the design points: main view of kit 2. 5. Other circumstances that need to be explained: The design product is a kit product, so it is used and sold as a set. Kit 1 is an inner packaging hose, kit 2 is an outer packaging box, and kit 1 is packed into kit 2 for sale and use.

The present application relates to a kind of recombinant human type III collagen and its preparation method and application, belong to the field of bioengineering technology.The amino acid sequence of the recombinant human type III collagen described in the present application is as shown in SEQ ID No.7, is through the directional arrangement of five characteristic triple helix domain and the expansion of polymerization, combined with high-density fermentation process of Pichia pastoris, successfully break through the technical barrier of 4.5g / L target protein concentration.The present application research shows that the recombinant protein at 0.5% concentration not only maintains more than 80% cell viability, more exhibits significant synergistic effect-promote fibroblast migration rate to increase more than 29% compared with blank control group, keratinocyte proliferation rate increases 40.62%, adhesion rate increases 6.53%, provides innovative solution for developing new collagen product with high-efficiency expression and multiple biological activities.

The invention belongs to the technical field of gene engineering, and provides a method for producing III-type collagen by bacillus subtilis and application of the III-type collagen, and the amino acid sequence of the III-type collagen is as shown in SEQ ID No.1. The III-type collagen has various biological activities such as repairing, moisturizing, anti-inflammation and anti-aging, the method for producing the III-type collagen by the bacillus subtilis has the advantages of high secretion efficiency and high yield, and the method is simple, low in cost and wide in application prospect.

This invention provides a medical collagen solution for wound repair and its preparation process, belonging to the field of collagen solution preparation technology. The preparation process includes the following steps: preparation of a zinc tea polyphenol complex; preparation of zinc tea polyphenol complex liposomes; preparation of thermosensitive composite microspheres; and preparation of the collagen solution. The collagen solution of this invention contains thermosensitive composite microspheres prepared by first encapsulating the zinc tea polyphenol complex with liposomes and then compounding it with recombinant human collagen type III. The thermosensitive composite microspheres are thermosensitive and can achieve intelligent release. Under human body temperature, the microspheres quickly release recombinant human collagen type III and zinc tea polyphenol complex liposomes, continuously providing nutrition and support for wound repair, which is beneficial for long-term wound repair and healing.

The present application relates to the technical field of collagen cosmetic, in particular to an anti-aging composition containing collagen, and a preparation method and application thereof. The anti-aging composition comprises recombinant human collagen type III and plant active ingredients, wherein the plant active ingredients at least include any one of fruiting body extract of NAEMATELIA AURANTIALBA and extract of GENTIANA TRIFLORA, and the anti-aging composition further comprises an auxiliary agent, wherein the auxiliary agent at least includes any one or more of trehalose, mannitol, carbomer, hydroxyethyl cellulose, glycerol, butanediol and pentanediol. The anti-aging composition can not only achieve a synergistic effect in terms of anti-aging effect, but also solve the problem of insufficient stability of recombinant human collagen type III to a certain extent through reasonable compounding of components.

The invention provides a collagen peptide-containing sebum membrane repairing composition and a preparation method thereof, and relates to the field of biomedical materials. The sebum film repairing composition is prepared from a recombinant III type collagen peptide, a recombinant I type collagen peptide, a ceramide compound, squalane, a natural moisturizing factor, glycosyl trehalose, phytosterol, glycerol, butanediol, polyglycerol-10 laurate, laurocapram and water. According to the sebum membrane repairing composition, the recombinant III-type collagen peptide and the recombinant I-type collagen peptide have a synergistic effect with the ceramide compound, so that the ceramide recovery rate is greatly improved, and the composition can realize'lipid barrier-aquaporin-tight connection 'three-in-one repairing of a sebum membrane.

The invention relates to the technical field of gene engineering, and discloses a recombinant human III-type collagen and a preparation method and application thereof.The amino acid sequence of the recombinant human III-type collagen comprises the amino acid sequence shown in SEQ ID NO.1. The amino acid sequence of the recombinant human III-type collagen has the advantages of being high in hydrophilicity, uniform in charge distribution and the like; due to the characteristic, the transdermal absorption efficiency of the product is greatly improved, so that the product can penetrate into the bottom layer of the skin more deeply, and a better effect is exerted. Meanwhile, due to the 100% humanized amino acid sequence design, the high affinity of the product and the human skin is ensured, and the risk of allergic reaction is reduced to the maximum extent.

The invention provides an anti-aging and anti-sugar folium cortex eucommiae plant extraction composition, a preparation method thereof and application of the anti-aging and anti-sugar folium cortex eucommiae plant extraction composition in oral beauty products, and belongs to the technical field of food. The composition is prepared from folium cortex eucommiae powder, herba cistanche powder, fructus corni powder and rhizoma gastrodiae powder by adopting deep eutectic solvent extraction, microbial fermentation, subcritical water extraction and ethanol reflux extraction processes respectively, and the extraction efficiency and bioavailability of active ingredients are remarkably improved. The composition disclosed by the invention is compounded with auxiliary materials such as III-type collagen peptide, vitamin C and the like to prepare a beautifying oral liquid, and the beautifying oral liquid has multiple beautifying effects of resisting saccharification, resisting oxidation, promoting collagen synthesis, enhancing skin barrier and the like. In-vitro, animal and clinical tests prove that the beautifying oral liquid has the effects of resisting saccharification and aging, increasing the thickness of an epidermal layer, improving skin barrier health and improving skin compactness.

The application belongs to the technical field of biological medicine, and provides a recombinant type III human collagen peptide for resisting HPV infection and a preparation method and application thereof, an amino acid sequence of which is a coding sequence of positions 991-1032 of type III human collagen UniProtKB: P02461 repeated three times, named as ATHPV3, and an amino acid sequence of which is shown as SEQ ID NO. 1. After expression and purification, the recombinant type III human collagen peptide with a molecular weight of about 13 kD is obtained, can be specifically combined with HPV 16 / 18 L1 protein, has multiple hydrogen bond actions, and can inhibit pseudo-virus infection of 293T cells at high and medium concentrations. The application successfully obtains a recombinant type III human collagen peptide with good purity, which can be combined with HPV 16 / 18 L1 and can effectively inhibit HPV 16 / 18 pseudo-virus infection of 293T cells, and provides an effective candidate drug for preventing and treating HPV infection.

The invention is applicable to the technical field of biological materials, and provides recombinant III-type collagen with a transdermal function and application thereof. The recombinant III-type collagen with the transdermal function comprises an oligopeptide segment with skin permeability, a flexible human III-type collagen segment located between the oligopeptide segment and a collagen functional structure segment, and a functional structure segment derived from human III-type collagen. According to the invention, an oligopeptide segment with a transdermal function is introduced into the molecular structure of the III-type collagen, so that the collagen keeps good biocompatibility and structural function, and meanwhile, the whole collagen obtains stable skin penetrating power, thereby expanding the application scene of the collagen in external application such as skin repair, aging resistance and skin care.

1. The name of the design product: packaging box (Wei Mei Hive Col 164.88° horizontal box). 2. The use of the design product: packaging box for recombinant humanized collagen type III gel for injection. 3. The design points of the design product: the combination of shape, pattern and color. 4. The picture or photo that best indicates the design points: perspective view. 5. The design claimed contains color.

The present application relates to the use of recombinant humanized collagen type III in the treatment of cervical cancer, in particular, the present application provides the use of recombinant humanized collagen type III in the preparation of products for preventing and / or inhibiting and / or treating cervical cancer; the amino acid sequence of the recombinant humanized collagen type III comprises n repeats of the sequence shown in SEQ ID NO. 1, n is an integer greater than or equal to 1, n is preferably 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 20, 24 or 32, wherein when n is an integer greater than or equal to 2, each repeat sequence is directly connected. Research data shows that the recombinant humanized collagen type III can significantly inhibit the proliferation and migration ability of cervical cancer cells, which provides help for the preparation and wide application of products for preventing and treating cervical cancer and the clinical treatment of cervical cancer.

The invention provides recombinant III-type collagen as well as a preparation method and application thereof, and belongs to the technical field of bioengineering. On the basis of human-derived III-type collagen COL3A1, an amino acid sequence suitable for efficient expression of pichia pastoris is obtained after optimization design and screening, a recombinant expression vector is provided on the basis, and recombinant engineering bacteria are constructed. The recombinant III-type micromolecular collagen is successfully expressed by adopting the constructed recombinant engineering bacteria, and the invention further provides a preparation method of the recombinant III-type micromolecular collagen. According to the present invention, the efficient expression of the recombinant III-type micromolecule collagen in the yeast is achieved, and the recombinant III-type micromolecule collagen has characteristics of high stability, and no significant degradation under different pH conditions, repeated freezing and thawing treatment, and different temperature and time duration treatment; the main band accounts for 90% or above in the fermentation stage, the purification difficulty is low, the sponge purity is 95% or above, and the cell adhesion activity is excellent while the stability is high.

1. The name of the design product: packaging box (Weiwei whitening flip cover box). 2. The use of the design product: packaging box for recombination of humanized collagen type III freeze-dried fiber. 3. The design points of the design product: the combination of shape, pattern and color. 4. The picture or photo that best indicates the design points: perspective view. 5. The design protected contains color.