61 results about "Collagen Type III" patented technology

The invention provides recombinant III-type collagen and application thereof, and relates to the technical field of genetic engineering. According to the invention, the yield of the collagen is improved by optimizing the nucleotide sequence of the target gene and optimizing the culture conditions of pichia pastoris, so that the collagen is efficiently expressed in engineering bacteria. The recombinant engineering bacterium shows strong potential in the aspect of improving the yield of protein with high economic value, and is suitable for large-scale industrial production. The recombinant III-type collagen is high in expression quantity, simple in preparation process and suitable for preparation of biological materials for medical cosmetology, wound repair and the like, and has a wide application prospect.

This invention discloses recombinant type III collagen, its preparation method, and its applications. The amino acid sequence of the recombinant type III collagen is shown in SEQ ID NO: 9. The recombinant type III collagen integrates the RGDV highly active motif and the EKEK hydrophilic stable domain with the core structure of type III collagen to achieve an integrated design of "high activity and long-lasting effect". The in vitro cell migration and collagen secretion effects are improved by more than 50% compared with wild type. Through anti-enzyme cleavage site modification and hydrophilic modification, the in vivo residence half-life is significantly prolonged to meet the long-term repair needs of the dermis.

The invention belongs to the technical field of bioengineering, and particularly relates to a kluyveromyces marxianus engineering strain for recombinant expression of hydroxylated human III-type collagen and application of the kluyveromyces marxianus engineering strain. The Kluyveromyces marxianus engineering strain of the human type III collagen is obtained by carrying out recombinant expression construction on proline hydroxylase and a human type III collagen gene in the Kluyveromyces marxianus; according to the invention, two truncation designs and codon optimization are carried out on the existing natural human III type collagen amino acid sequence to obtain the human III type collagen C3S1 and C3S2, and the recombinant secretory expression in the kluyveromyces marxianus engineering strain is realized. Like hydroxylation modification of the human III type collagen, the human III type collagen C3S1 and the human III type collagen C3S2 which are subjected to recombinant expression have proline hydroxylation characteristics. The recombinant human III-type collagen is prepared by using the kluyveromyces marxianus engineering strain, the method is simple, the production efficiency is high, and large-scale production is easy.

The invention discloses a composition of type III collagen and beta-glucan and application of the composition in repairing vaginal mucosa injury, and belongs to the technical field of biological medicine, and the composition comprises the following effective components: type III collagen with the concentration of 0.3-1.8 mg / mL and beta-glucan with the mass concentration of 0.1-0.8%. The collagen III provides structural support required by tissue repair, the beta-glucan improves inflammation microenvironment, regulates immune response and jointly promotes proliferation and migration of damaged vaginal epithelial cells, the cell proliferation rate and the scratch healing rate are far higher than those of a group using any component independently, and rapid repair of mucosal lesions can be achieved.

The invention discloses recombinant human III-type collagen and application thereof, and belongs to the technical field of biochemical engineering. The method comprises the following steps: according to human III-type collagen, obtaining a target amino acid sequence through splitting, and integrating the target amino acid sequence into an expression vector to obtain plasmids; transforming the plasmid into a host, and screening positive clones to obtain an expression strain; and culturing the expression strain, and separating and purifying to obtain the high-yield and high-purity human III-type collagen. The obtained recombinant human III-type collagen has a collagen triple helix region characteristic sequence (Gly-X-Y) n, has good performances of promoting cell migration and adhesion and preventing light damage, and can be widely applied to the fields of medicines, biological materials, health care products, cosmetics and the like.

The application discloses recombinant collagen type III, a hydrogel, a preparation method and application, and belongs to the technical field of medical materials. The protein has an amino acid sequence as shown in SEQ ID No. 2 or 3, and has excellent biocompatibility and cell activity. The hydrogel based on the protein is formed by mixing a solution of the protein with a solution containing oxidized sodium alginate and hydroxypropyl methyl cellulose, adjusting pH, sequentially introducing an EDC / NHS crosslinking system and calcium ions, and standing. The process constructs a stable double-network structure of EDC / NHS catalyzed covalent crosslinking network and OSA-Ca 2+ ion crosslinking network synergistic effect. The obtained hydrogel has high mechanical strength, excellent toughness, long-lasting wet tissue adhesion and suitable swelling performance, can effectively adapt to the complex stress changes of dynamic wounds, and provides an active matrix for cells, and promotes tissue repair.

This invention belongs to the field of biomedical technology, specifically relating to a delivery system for type III collagen mRNA and its applications. The nucleic acid delivery system of this invention includes a delivery carrier and a delivered nucleic acid, wherein the nucleic acid is collagen mRNA, and the delivery carrier includes one or more of modified amino acids, growth factors, and polymeric materials. Through intradermal injection, the delivery system of this invention can be administered to the dermal layer of the skin and enter relevant cells, utilizing endogenous cellular proteins to achieve mRNA transfection, forming natural human type III collagen. This invention avoids the immunogenicity risks and structural inactivation problems associated with exogenous collagen injections, and can efficiently and safely replenish non-regenerative type III collagen in tissues. It can be used for various indications such as treating skin aging, promoting wound healing, reducing scar formation, and improving vascular elasticity.

The invention relates to an mRNA (messenger Ribonucleic Acid) molecule and application thereof in preparation of beauty and skin care products, and particularly, the mRNA molecule comprises a nucleic acid sequence for coding type III collagen. After the mRNA provided by the invention is applied to the skin, the skin cells can generate collagen, and the aged skin is normalized by increasing the collagen content in the skin tissue, so that the effect of repairing the skin is achieved.

The invention relates to the technical field of gene engineering, and discloses a recombinant human III-type collagen and a preparation method and application thereof.The amino acid sequence of the recombinant human III-type collagen comprises the amino acid sequence shown in SEQ ID NO.1. The amino acid sequence of the recombinant human III-type collagen has the advantages of being high in hydrophilicity, uniform in charge distribution and the like; due to the characteristic, the transdermal absorption efficiency of the product is greatly improved, so that the product can penetrate into the bottom layer of the skin more deeply, and a better effect is exerted. Meanwhile, due to the 100% humanized amino acid sequence design, the high affinity of the product and the human skin is ensured, and the risk of allergic reaction is reduced to the maximum extent.

The invention provides a composition for preventing or treating radioactive skin injury and application of the composition. The composition comprises (1) recombinant III type collagen; (2) component (a): at least one hyaluronic acid or a salt thereof with a molecular weight of 1000-2500k Da; (3) a component (b): at least one hyaluronic acid or a salt thereof with a molecular weight of 200-500 k Da; the component (a), the component (b) and the component (c) form a network structure, so that an excellent external environment can be provided for the collagen, the recombinant collagen can promote cell proliferation and migration, and the component (a), the component (b) and the component (c) have a mutual synergistic effect.

The invention discloses a water-based skin care product containing type IV collagen and a preparation method of the water-based skin care product. The water-based skin care product comprises the recombinant collagen skin care composition and a water-based matrix. The recombinant collagen skin care composition comprises a recombinant IV type collagen, a GLP tripeptide, a PGD tripeptide, a recombinant III type collagen, a recombinant I type collagen and a recombinant human-like collagen according to a specific ratio. According to the invention, the recombinant collagen composition is creatively applied to a water-based skin care product to maintain the collagen of the whole skin layer, so that the effect of comprehensively promoting skin barrier repair is achieved.

The invention provides an oral preparation for improving anemia after gastric resection operation and a preparation method thereof, and relates to the technical field of biological medicines. The oral preparation is prepared from the following raw materials: vitamin B12, internal factors, chickpea protein, III type collagen peptide, a pH regulator, an antioxidant and a dispersing agent. The vitamin B12 and the internal factors are taken as active ingredients, the utilization degree of the vitamin B12 is remarkably improved through the specific proportion of the vitamin B12 and the internal factors, and the prepared reagent meets the requirement that the reagent is not released in a gastric acid environment and then is slowly released in the intestinal tract, and has a remarkable effect on the anemia problem caused by deficiency of the vitamin B12 after the gastric resection operation; and the stability of the medicine is high.

The application discloses a liquid for repairing oral mucosa injury and belongs to the technical field of mucosa repair. The liquid comprises seabuckthorn seed oil extract, Periplaneta americana active polypeptide, recombinant human collagen type III, polyoxyethylene 40 hydrogenated castor oil, poloxamer, propylene glycol, glycerol, dipotassium glycyrrhizinate, sucralose, a cooling agent, nipagin methyl ester, nipagin propyl ester, essence, and purified water. The liquid prepared by the application has a smooth and soft taste, a sweet and pleasant flavor, a fresh and clean breath after spraying / flushing, good product stability, no mucosa irritation, and can quickly repair damaged mucosa, and the preparation method is simple, so that the liquid can be used for industrial large-scale production.

1. The name of the design product: packaging kit (medical recombinant humanized type III collagen dressing). 2. The use of the design product: for product packaging. 3. The design points of the design product: in the pattern. 4. The picture or photo that best indicates the design points: main view of kit 2. 5. Other circumstances that need to be explained: The design product is a kit product, so it is used and sold as a set. Kit 1 is an inner packaging hose, kit 2 is an outer packaging box, and kit 1 is packed into kit 2 for sale and use.

The present application relates to a kind of recombinant human type III collagen and its preparation method and application, belong to the field of bioengineering technology.The amino acid sequence of the recombinant human type III collagen described in the present application is as shown in SEQ ID No.7, is through the directional arrangement of five characteristic triple helix domain and the expansion of polymerization, combined with high-density fermentation process of Pichia pastoris, successfully break through the technical barrier of 4.5g / L target protein concentration.The present application research shows that the recombinant protein at 0.5% concentration not only maintains more than 80% cell viability, more exhibits significant synergistic effect-promote fibroblast migration rate to increase more than 29% compared with blank control group, keratinocyte proliferation rate increases 40.62%, adhesion rate increases 6.53%, provides innovative solution for developing new collagen product with high-efficiency expression and multiple biological activities.

The invention belongs to the technical field of gene engineering, and provides a method for producing III-type collagen by bacillus subtilis and application of the III-type collagen, and the amino acid sequence of the III-type collagen is as shown in SEQ ID No.1. The III-type collagen has various biological activities such as repairing, moisturizing, anti-inflammation and anti-aging, the method for producing the III-type collagen by the bacillus subtilis has the advantages of high secretion efficiency and high yield, and the method is simple, low in cost and wide in application prospect.

The present application relates to the technical field of collagen cosmetic, in particular to an anti-aging composition containing collagen, and a preparation method and application thereof. The anti-aging composition comprises recombinant human collagen type III and plant active ingredients, wherein the plant active ingredients at least include any one of fruiting body extract of NAEMATELIA AURANTIALBA and extract of GENTIANA TRIFLORA, and the anti-aging composition further comprises an auxiliary agent, wherein the auxiliary agent at least includes any one or more of trehalose, mannitol, carbomer, hydroxyethyl cellulose, glycerol, butanediol and pentanediol. The anti-aging composition can not only achieve a synergistic effect in terms of anti-aging effect, but also solve the problem of insufficient stability of recombinant human collagen type III to a certain extent through reasonable compounding of components.

The invention relates to the technical field of gene engineering, and discloses a recombinant human III-type collagen and a preparation method and application thereof.The amino acid sequence of the recombinant human III-type collagen comprises the amino acid sequence shown in SEQ ID NO.1. The amino acid sequence of the recombinant human III-type collagen has the advantages of being high in hydrophilicity, uniform in charge distribution and the like; due to the characteristic, the transdermal absorption efficiency of the product is greatly improved, so that the product can penetrate into the bottom layer of the skin more deeply, and a better effect is exerted. Meanwhile, due to the 100% humanized amino acid sequence design, the high affinity of the product and the human skin is ensured, and the risk of allergic reaction is reduced to the maximum extent.

The invention is applicable to the technical field of biological materials, and provides recombinant III-type collagen with a transdermal function and application thereof. The recombinant III-type collagen with the transdermal function comprises an oligopeptide segment with skin permeability, a flexible human III-type collagen segment located between the oligopeptide segment and a collagen functional structure segment, and a functional structure segment derived from human III-type collagen. According to the invention, an oligopeptide segment with a transdermal function is introduced into the molecular structure of the III-type collagen, so that the collagen keeps good biocompatibility and structural function, and meanwhile, the whole collagen obtains stable skin penetrating power, thereby expanding the application scene of the collagen in external application such as skin repair, aging resistance and skin care.

1. The name of the design product: packaging box (Wei Mei Hive Col 164.88° horizontal box). 2. The use of the design product: packaging box for recombinant humanized collagen type III gel for injection. 3. The design points of the design product: the combination of shape, pattern and color. 4. The picture or photo that best indicates the design points: perspective view. 5. The design claimed contains color.

The present application relates to the use of recombinant humanized collagen type III in the treatment of cervical cancer, in particular, the present application provides the use of recombinant humanized collagen type III in the preparation of products for preventing and / or inhibiting and / or treating cervical cancer; the amino acid sequence of the recombinant humanized collagen type III comprises n repeats of the sequence shown in SEQ ID NO. 1, n is an integer greater than or equal to 1, n is preferably 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 20, 24 or 32, wherein when n is an integer greater than or equal to 2, each repeat sequence is directly connected. Research data shows that the recombinant humanized collagen type III can significantly inhibit the proliferation and migration ability of cervical cancer cells, which provides help for the preparation and wide application of products for preventing and treating cervical cancer and the clinical treatment of cervical cancer.

The invention provides recombinant III-type collagen as well as a preparation method and application thereof, and belongs to the technical field of bioengineering. On the basis of human-derived III-type collagen COL3A1, an amino acid sequence suitable for efficient expression of pichia pastoris is obtained after optimization design and screening, a recombinant expression vector is provided on the basis, and recombinant engineering bacteria are constructed. The recombinant III-type micromolecular collagen is successfully expressed by adopting the constructed recombinant engineering bacteria, and the invention further provides a preparation method of the recombinant III-type micromolecular collagen. According to the present invention, the efficient expression of the recombinant III-type micromolecule collagen in the yeast is achieved, and the recombinant III-type micromolecule collagen has characteristics of high stability, and no significant degradation under different pH conditions, repeated freezing and thawing treatment, and different temperature and time duration treatment; the main band accounts for 90% or above in the fermentation stage, the purification difficulty is low, the sponge purity is 95% or above, and the cell adhesion activity is excellent while the stability is high.

1. The name of the design product: packaging box (Weiwei whitening flip cover box). 2. The use of the design product: packaging box for recombination of humanized collagen type III freeze-dried fiber. 3. The design points of the design product: the combination of shape, pattern and color. 4. The picture or photo that best indicates the design points: perspective view. 5. The design protected contains color.

The invention discloses a yeast heterologous expression type III recombinant human collagen peptide and a preparation method thereof. The recombinant human collagen peptide comprises an amino acid sequence as shown in SEQ ID No. 1. The multi-copy tandem repeat peptide comprises a basic repeat unit as shown in SEQ ID No. 1. The preparation method comprises the following steps: connecting a nucleic acid molecule for coding the multi-copy tandem repeat peptide into a vector to construct a recombinant expression vector plasmid, and transferring the recombinant expression vector plasmid into a host cell to obtain a multi-copy positive transformant; culturing a multi-copy positive transformant, and performing induction treatment; performing centrifugation, microfiltration, ultrafiltration and purification on the fermentation liquor to obtain multi-copy tandem repeat peptide; carrying out enzymolysis on the multi-copy tandem repeat peptide, and then carrying out ultrafiltration, collection and purification. According to the invention, human collagen amino acid sequences are screened, and specific amino acids are replaced, so that the influence caused by glycosylation and non-specific combination of MMP-1 enzyme cutting sites and lysine sites is avoided, and the biological activity and the utilization degree are remarkably improved.

The invention relates to the field of biomedical materials, in particular to Tibetan sheep embryo type III collagen as well as a preparation method and application of the Tibetan sheep embryo type III collagen. Comprising the following steps: sequentially carrying out cation exchange chromatography purification and gel chromatography purification on an extracting solution which is extracted from a Tibetan sheep embryo and contains type III collagen; in the cation exchange chromatography purification step, a sample is loaded in an acetic acid buffer solution with the pH value of 2.5-3.5 and the concentration of 0.4-0.6 M, an eluent is a sodium chloride solution, and gradient elution is carried out within the eluent concentration range of 0-1 mol / L; in the step of gel chromatography purification, an eluent is a phosphate buffer solution with the pH value of 7.2-7.4, and the concentration of the eluent is 0.01-0.1 M; type III collagen with a complete triple helix structure and high biological activity is prepared from Tibetan sheep embryonic tissue, has the properties of good biocompatibility, good thermal stability, promotion of cell proliferation, adhesion and migration and the like, and has high medical value.

The invention discloses recombinant III-type collagen with thermal stability as well as a preparation method and application thereof, and belongs to the field of medical materials. The amino acid sequence of the recombinant III type collagen with thermal stability is any one of SEQ ID NO: 4-6. Based on rational design and functional optimization of the amino acid sequence, a key active sequence and a triple helix structure which are consistent with those of natural human body collagen are completely reserved; meanwhile, efficient and controllable large-scale preparation by utilizing an escherichia coli expression system is realized. The recombinant III-type collagen obtained by the invention shows excellent thermal stability, and a stable, reliable, economical and feasible raw material solution meeting strict quality control requirements is provided for large-scale and industrial application of the collagen in the fields of medical instruments, functional skin care products, tissue engineering and the like.

This invention relates to the fields of microbial fermentation and genetic engineering technology, specifically disclosing a method for preparing human type III collagen fragments. Based on Pichia pastoris, this invention constructs a multidimensional synergistic expression system of "molecular chaperone co-expression - precursor supply - osmotic pressure adaptation regulation," reducing the structural instability of collagen fragments during secretion and post-processing, and constructing an expression system suitable for stable and efficient secretion of collagen fragments. The method described in this invention can achieve efficient extracellular secretory expression of human type III collagen fragments, and the obtained protein has high structural uniformity and stability, with a protein yield of up to 6.55 g / L under 5 L fermenter conditions. The method described in this invention is suitable for the large-scale preparation of human type III collagen fragments and provides a new technical solution for obtaining collagen products with high structural uniformity and good functional activity.

The application discloses a kind of circular RNA of expressing collagen type III polypeptide and application thereof, belong to collagen preparation technical field.The application provides a kind of circular recombinant RNA by no-scar design, element screening, with high translation efficiency, good cyclization effect, strong expression stability, more durable, more close to nature, nucleotide chain is sequentially connected by promoter, 5'homologous arm, 3'intron, IRES sequence, target protein coding sequence, 5'intron, 3'homologous arm, nucleotide sequence is as shown in SEQ ID NO.8;Circular recombinant RNA can successfully express collagen type III peptide in ex vivo human fibroblast, mouse fibroblast and mouse in vivo, to a certain extent, reverse ultraviolet light-induced photoaging process, improve skin photoaging damage and promote tissue repair, with high cell uptake rate, strong expression stability, good persistence;Low immunogenicity, good biocompatibility and in vitro safety.

The application provides a kind of stable recombinant collagen type III and its application, the recombinant collagen type III includes the amino acid sequence as shown in SEQ ID NO.1.The application efficiently prepares the recombinant human collagen type III by genetic engineering technology, and high purity product is obtained by combining purification process.The recombinant human collagen type III has excellent water solubility and stability, and can be stored at room temperature for more than one year.In addition, the completely humanized sequence eliminates the immunogenicity and pathogen contamination risk of animal-derived collagen, ensuring the safety of application, and has excellent effects of inhibiting hyaluronidase, cell adhesion, repair, soothing and the like.The application provides a new collagen raw material for food, medicine, health care products, cosmetics, medical device materials and scientific research fields, and has wide application prospect.

The invention discloses an mRNA (messenger Ribonucleic Acid) medicine of type III collagen and application of the mRNA medicine, and belongs to the technical field of biological medicines. The method comprises the following steps: carrying out mRNA (messenger ribonucleic acid) coding optimization design on the basis of an amino acid sequence of human type III collagen, and carrying out in-vitro expression screening to obtain a candidate molecule Conus001 with high expression performance. Wild-type mouse in-vivo experiments prove that the molecule can significantly improve the expression level of III-type collagen, and subsequent mouse pharmacodynamic studies show that the molecule has a definite ultraviolet damage protection function. Experimental data reveals that through gene delivery of Conus001, expression of III-type collagen is promoted, ultraviolet damage resistance of an organism can be effectively improved, and skin quality and skin elasticity can be recovered. A novel treatment scheme is provided for delaying senescence of human beings.