43 results about "Three dimensional scaffolds" patented technology

The invention provides a preparation method of a cell culture three-dimensional scaffold and cell culture meat, which comprises the following steps: firstly, preparing a gelatin-carrageenan mixed solution and a gel precursor solution of transglutaminase, dividing into two parts, incubating at constant temperature to obtain two parts of composite hydrogel, randomly freeze-drying one part to obtain aerogel, and freeze-drying the aerogel to obtain the cell culture three-dimensional scaffold. Stacking on the other part and incubating at constant temperature again to obtain the prefabricated double-layer gel. The prefabricated double-layer gel is directionally frozen, ice crystals grow upwards from the bottom, freezing is stopped after the temperature is reduced to the set temperature, and the cell culture three-dimensional scaffold is obtained through drying. The stent adopts an ordered-disordered partition design, parallel micro-channels are formed in the lower layer by using a directional ice template method, and muscle fiber arrangement is simulated; a porous spongy structure is constructed on the upper layer by using a random ice template method, and is adapted to adipocyte proliferation. Two layers of interfaces are interpenetrated and communicated, natural mechanical gradients are combined, natural tissue characteristics are re-engraved, the traditional limitation is broken through, the cell co-culture efficiency and the cultured meat quality are improved, and industrialization is promoted.

Novel nanoconjugate compositions, such as nanoparticle-oligomer arrays (NOAs), and related methods for precision therapeutics and molecular diagnostics. These compositions comprise a core, optionally a nanoparticle core, and a plurality of oligomeric strands forming a dense, highly oriented three-dimensional scaffold. This scaffold architecture is engineered to position functional molecules precisely at desired distances from the core and / or the external surface, thereby controlling their physical and chemical interactions with the core and / or microenvironment, enabling distinct functionalities not observed with the functional molecules in an unstructured formulation.

A method is provided. The method includes: generating light by a plasma of a light source of a semiconductor processing tool; generating patterned light by a mask assembly, the patterned light including the light reflected by a pattern of the mask assembly; during generating the patterned light, protecting the mask assembly by a pellicle assembly including a pellicle membrane, the pellicle membrane including a nanotube-based scaffold structure having nanotubes bound together by a capping layer; and performing a semiconductor process on a semiconductor wafer by the patterned light.

The present disclosure provides, among other things, a method of isolating and expanding gamma delta (γδ) T cells, wherein the method comprises: (a) isolating non-hematopoeitic tissue by biopsy or explant, and (b) culturing the isolated non-hematopoeitic tissue in the absence of a three-dimensional scaffold or grid, thereby expanding and isolating gamma delta (γδ) T cells.

The present disclosure pertains to a 3D scaffold for cell growth and proliferation. In particular, the present disclosure provides a method of producing an artificial 3D scaffold to support stem cell growth and later their differentiation, by converting biodegradable amphiphilic copolymers (star polymer) into nanowire scaffolds, through a molecular self-assembly process. The invention also relates to the use of said scaffold for cell culture and / or transplantation.

According to the self-energized biological 3D printing bio-ink, the 3D printing bioactive scaffold and the preparation method of the self-energized biological 3D printing bio-ink, the controllable-release self-energized biological 3D printing bio-ink is prepared by mixing the organic high-molecular polymers with different molecular weights and the energy metabolism promoting substances, and the ink is good in biocompatibility; by changing the content of energy supply substances, the ink can be designed to'provide 'and'accept' different cell metabolism regulation clues in an adjustable time regulation manner, better migration and adhesion of cells are realized, and functions are exerted, and meanwhile, the ink has controllable slow release of energy metabolism promoting substances and generates micro-nano pores when being used for constructing a three-dimensional bracket through 3D printing, so that the energy metabolism promoting effect is improved. The hydrophilicity of the scaffold is improved, the biological energy metabolism activity of the composite material is enhanced, the energy metabolism promoting substance can provide a sufficient nutrition environment for cell growth, the cell energy metabolism process is regulated and controlled, and adhesion, growth, proliferation and functionalization of cells are facilitated.

Described herein are three-dimensional scaffold compositions and methods for bone repair or regeneration. In some embodiments, the disclosed scaffolds comprise a core portion mimicking a native trabecular bone structure that is surrounded by an exterior portion mimicking a native cortical bone structure. In some embodiments, the scaffolds may be functionalized by mineralization and / or pre-vascularization to promote bone and blood vessel formation.

A sterilized PLA-collagen scaffold comprising animal collagen and a method for preparing a sterilized scaffold for medical use, the method comprising the steps of:i) Loading animal collagen to a fiber mesh containing fibers of polylactide polymer or copolymer (commonly denoted PLA) to obtain a PLA-collagen scaffold,ii) Drying the PLA-collagen scaffold obtained from step i),iii) Sterilizing the PLA-collagen scaffold obtained from the drying step ii) to obtain the sterilized scaffold.The sterilized scaffold obtained has improved biomechanical and physical properties compared with an unsterilized scaffold.

The application belongs to the technical field of bone tissue engineering, and particularly relates to a recombinant collagen-chitosan-PEG-hydroxyapatite bone repair scaffold material, a preparation method and application. The bone repair scaffold material is formed into a one-way ordered three-dimensional scaffold by a directional freezing method through recombinant collagen, chitosan and PEG. Nano-hydroxyapatite is uniformly deposited in the inside and surface through a special mineralization process. The prepared material has a very good bionic natural bone structure and composition, a good reticular structure and mechanical strength, good biocompatibility and cell activity, a significant repair effect on critical size bone defects, a good osteogenic induction effect and bone conductivity. The bone repair scaffold material provides a new potential treatment method for severe bone defects, and has a wide application in the fields of artificial bone, artificial cartilage, biological scaffolds and the like.

A method of preparing a three-dimensional scaffold comprises obtaining fish scales including a plurality of microgrooves and ridges having a width ranging from about 25 μM to about 30 μM and sterilizing the fish scales to provide the three-dimensional scaffold. The three-dimensional scaffold can be used for in vitro cell growth.

The present invention is directed to a method for ex-vivo-engineering of cells, in particular stem cells or T cells, preferably hematopoictic stem and / or progenitor cells, mesenchymal stem cells, or T cells comprising a step of culturing the cells on a three-dimensional scaffold. The method of the invention is capable of improving the efficiency of genetic modification of cells and the functionality of the engineered cells.

Device (1), and method for manufacturing the same, for detecting the growth of a three-dimensional cell cluster (C), comprising: a substrate (2) having a support surface (21); a plurality of electrodes (3) arranged according to a predetermined spatial distribution, for detecting an electrical potential in the presence of a three-dimensional cell cluster (C); a plurality of three-dimensional scaffolds (4), comprising at least a first scaffold (41), arranged at a respective measuring electrode (31), and at least a second scaffold (42), arranged at a respective reference electrode (32), wherein the at least one first scaffold (41) has a proliferation conformation, adapted to promote the growth of the three-dimensional cell cluster (C), and the at least one second scaffold (42) has an inhibition conformation adapted to prevent the growth of the three- dimensional cell cluster (C).

A three dimensional scaffold for generating cell or protein assemblies. This degradable scaffold can be applied to various types of cells. Also disclosed are methods of treating a condition by implanting the protein or cell assembly prepared according to the method described herein.

The present disclosure relates generally to chemical sensors and polymer coatings for chemical sensors, and more particularly to a sensing system including a sensor having a polymeric 3D printed coating disposed on a surface thereof. A sensing system includes a sensor including a resonator structure. The sensing system includes a polymeric 3D printed coating disposed on a surface of at least a portion of the sensor. The polymeric 3D printed coating includes a three-dimensional scaffold structure.

An in vitro construct useful for toxicity testing is provided, comprising: a three-dimensional (3D) scaffold comprising silk fibroin and having a crosslinked porous matrix; and stem cells adherent to the 3D scaffold. In some embodiments, the stem cells adherent to the 3D scaffold maintain stable mitochondrial DNA in long term culture. In some embodiments, the stem cells are urine stem cells.

The present disclosure relates to a three-dimensional scaffold comprising a plurality of scaffold elements, each scaffold element comprising at least one amino acid peptide component and at least two nucleic acid components, the peptide component consisting of a length of amino acid wherein the at least two nucleic acid component portions of the plurality of scaffold elements are configured to mediate self-assembly of the scaffold.

The invention relates to the technical field of biomedicine, in particular to a mesenchymal stem cell differentiation method for promoting bone joint repair, which comprises the following steps: inoculating mesenchymal stem cells on a three-dimensional scaffold material to form a complex, applying an alternating electric field within a specific parameter range for stimulation, and combining with growth factor culture to realize directional differentiation. According to the method, by regulating and controlling the frequency, the voltage amplitude and the action time of the alternating electric field, the differentiation efficiency of the cells in the cartilage or osteogenesis direction is remarkably improved, and meanwhile the adaptability of the cells in the complex bone joint microenvironment is enhanced.

The invention provides a multifunctional artificial eye seat with polar radiation aperture distribution and a preparation method thereof, and belongs to the technical field of biomedical materials. The artificial eye seat is a porous spherical or ellipsoidal stent taking trace copper doped zinc melilite biological ceramic as a main component. The internal micropores are mutually communicated curved surface pore units, the pore diameters of the micropores are radially expanded from one end of the spherical surface to the opposite side in a macropore-mesopore-micropore gradient layout, a polar radiation pore diameter distribution three-dimensional bracket model is printed by adopting photocuring ceramic, and then the polar radiation pore diameter distribution three-dimensional bracket model is cleaned, dried and sintered to form the polar radiation pore diameter distribution three-dimensional bracket; the artificial eye seat gives consideration to the anatomical physiological structure of the orbit, the surface of the macropore area is attached to the deep soft tissue of the orbit, and the micropore area faces the ocular surface, so that vascularized soft tissue ingrowth is efficiently promoted, infection is durably resisted, common risks such as displacement and exposure of the artificial eye seat are effectively overcome, and the artificial eye seat has good application value in orbit reconstruction and reshaping.

Engineered tissue models based on three-dimensional (3D) scaffolds, also referred to herein as tissue-engineered 3D models, can be used as in vitro diagnostic and drug screening tools for predicting, preventing and / or treating cancer metastases.

Method for the production of extracellular vesicles, EVs, loaded with a molecule of interest in a bioreactor containing a porous three-dimensional, 3D, scaffold, wherein the method comprises a seeding of the scaffold with a starting number of adherent immortalized cells resuspended after detachment, an attachment of the cells on said scaffold, an expansion of the cells on said scaffold up to a second number of adherent cells, defining a multiplication factor of at least 5 for a single expansion step, a production of EVs by said cells, a phase of loading said EVs with said molecule of interest, a harvest of EVs.

A microfluidic device includes multiple microfluidic assay units arranged on a substrate, with each assay unit including multiple scaffold regions each containing a three-dimensional scaffold with associated cells, and a media channel surrounding a fluid-permeable boundary portion of at least a second scaffold region, wherein a fluid-permeable interface between the media channel and the second scaffold region comprises a curved shape spanning an arc of more than 90 degrees. A third scaffold region may be provided. Boundaries between different scaffold regions, and between a scaffold region and the media channel, may include microposts that may be spaced apart in a curved configuration. A method for performing an assay utilizing such a device is further provided.

The invention discloses a three-dimensional bracket and a preparation method thereof. The three-dimensional scaffold is of a three-dimensional porous network structure formed by interweaving silicon dioxide nanofibers, and the three-dimensional scaffold is prepared by performing gas foaming treatment on a silicon dioxide nanofiber membrane; an epigallocatechin gallate-metal ion composite coating can be further loaded on the surface of the stent, and metal ions are preferably copper ions. The preparation method comprises the following steps: preparing PVA and TEOS into a spinning solution, and carrying out electrostatic spinning and high-temperature calcination to obtain a silicon dioxide nanofiber membrane; and then soaking in a sodium borohydride solution for gas foaming treatment to construct the three-dimensional porous scaffold. Structural transformation from a two-dimensional membrane to a three-dimensional through porous scaffold is achieved through mild gas foaming, the material is endowed with high water absorption, remarkable antibacterial and antioxidant activity and excellent angiogenesis and osteogenesis promoting performance by combining with the active coating, and the material has good application prospects in the field of tissue regeneration engineering.

A system for collecting biomarkers from a fluid stream includes a collection vessel having at least one fluid port that is adapted to be in fluid communication with the fluid stream. The collection vessel defines an inner volume. A 3D scaffold is disposed within the inner volume of the collection vessel. The 3D scaffold is chemically functionalized to bind the biomarkers in the fluid stream, wherein the biomarkers are eukaryotic cells of interest, extracellular vesicles associated with the eukaryotic cells of interest, or combinations thereof. A fluid driver circulates the fluid stream through the collection vessel via the at least one fluid port such that the fluid stream interacts with the 3D scaffold.

The invention relates to the technical field of biomedical engineering, and discloses a dynamic culture device and a dynamic culture method.The dynamic culture device comprises a culture tank, culture dishes, a disturbance device, a monitoring module and a control system, the multiple culture dishes with through holes are arranged in the vertical direction, and the disturbance device drives a magnetic rotor to rotate through magnetic force; the monitoring module is used for detecting and adjusting culture environment parameters, and the control system controls operation of the monitoring module and the magnetic driving device. Under the disturbance of the magnetic rotor, the culture medium forms a dynamic flow field and flows through the culture dishes, the flow field penetrates through the through holes of the culture dishes to promote the culture medium to circulate among the culture dishes, so that the mechanical stimulation response of the three-dimensional scaffold containing the cells is enhanced, the secretion activity of the cells is promoted to be activated, and the dynamic flow field can also effectively remove metabolic wastes and improve the activity of the cells. Nutrients are supplemented in real time, so that the production efficiency of cell active materials is improved. The monitoring module and the control system realize controllability of culture environment parameters, and improve stability and consistency of production of cell active materials.

The invention relates to the technical field of biotechnology and cell engineering, in particular to a large-scale culture method of central memory T cells (TCM). According to the method, the lymphocyte expressed by the high homing receptor is activated by adopting an alternating electric field, and the cell activity is improved by combining with a composite metabolism regulator. A two-photon excitation three-dimensional scaffold of a modified collagen peptide, ferroferric oxide and a quantum dot labeled organoid is utilized to simulate an in-vivo microenvironment to promote cell anchoring. And finally, through gene editing, engineering a cryopreservation system of saccharomyces cerevisiae and ultraviolet light activated autophagic peptide, so as to realize the enhancement of cell function stability and long-term memory characteristic. According to the invention, the limitation of traditional two-dimensional culture is broken through, and an efficient and controllable T cell preparation scheme is provided for tumor immunotherapy, autoimmune disease regulation and personalized cell therapy through systematic integration of physical field regulation, bionic scaffold design and genetic engineering means.

Provided are biospheres comprising: (a) a three-dimensional scaffold matrix for biological growth; (b) a first type of cell configured to be in contact with the three-dimensional scaffold matrix to grow to generate the biosphere with an internal volume; and (c) a second type of cell disposed in the internal volume. Also provided are methods of creating the same.

Synthetic cellular support systems in the form of engineered extracellular matrices are provided. The cellular support system may include a three-dimensional scaffold structure comprising at least one void. At least one suspended fibril spans across the at least one void in the three-dimensional scaffold structure. The suspended fibril comprises at least one extracellular matrix protein, such as fibronectin, and at least one glycan, such as a hyaluronic acid. The suspended fibril is capable of supporting cells and promoting three-dimensional cellular growth. In various aspects, a plurality of suspended fibrils may span the void to form a three-dimensional suspended fibrillar network.

The invention discloses a method and a system for printing a silk fibroin scaffold by using ultrasound, and belongs to the technical field of biological material manufacturing and tissue engineering. The method comprises the following steps: preparing silk fibroin bio-ink, polydopamine-coated barium titanate composite powder and lipid microbubbles; mixing the three components to obtain composite printing ink; and sequentially applying ultrasonic waves to predetermined three-dimensional point positions in a tool loaded with the ink by using a focused ultrasonic transducer, triggering a local cavitation effect under the assistance of microbubbles, inducing silk fibroin to solidify at a fixed point, and accumulating point by point to form a three-dimensional bracket. The invention further provides an ultrasonic printing device for implementing the method. The preparation method avoids the use of a chemical cross-linking agent, and is green and safe; the cavitation threshold value is reduced through microbubbles, and low-energy and high-efficiency solidification is achieved; fine printing of a complex structure is achieved by combining focused ultrasound and three-dimensional motion control. And a piezoelectric function is integrated, so that the prepared stent has good biocompatibility and bioelectricity activity.

This invention discloses a three-dimensional visualization organoid culture device, belonging to the field of organoid culture technology. It includes a control center, which is communicatively connected to a virtual three-dimensional scaffold module, a laser visualization monitoring module, a biolayer interference sensing module, and an automated culture medium control module. This invention abandons the traditional matrix gel solid scaffold and employs focused acoustic vortex technology to construct a dynamically adjustable virtual acoustic trap array. It utilizes acoustic radiation force to achieve cell capture and spatial arrangement, avoiding the interference of uncertainties in the composition and biocompatibility differences of solid scaffolds on organoid growth. Simultaneously, the acoustic trap array can adaptively reconstruct its topology according to the cell aggregation stage and metabolic state, solving the problem that traditional scaffold structures cannot adapt to dynamic cell growth, improving the morphological uniformity and physiological relevance of organoids, and providing a high-quality culture medium for organoid research and clinical applications.

A method for prepring a porous aerogel scaffold includes: adding a photoinitiator and polyethylene glycol diacrylate in a buffer solution, dissolving by heating and evenly mixing, adding Pluronic® F127 (Poloxamer 407) into the mixed solution, and standing at a low temperature to obtain an aerogel scaffold material; printing a hydrogel scaffold by using a 3D printing technology, and performing UV irradiation so that a cross-linking of the hydrogel scaffold is caused to form a three-dimensional scaffold with a stable structure, performing low-temperature soaking to remove Pluronic® F127 (Poloxamer 407), and then freeze drying the three-dimensional scaffold to obtain the porous aerogel scaffold. Wherein, Pluronic® F127 (Poloxamer 407) serves as a sacrificial material which is removed after the 3D printing of the hydrogel scaffold is completed, and then a porous structure can be formed in the scaffold in combination with a freeze drying technology, which facilitates the survival, growth and proliferation of cells during the three-dimensional culture.