54 results about "Three dimensional scaffolds" patented technology

A three-dimensional scaffold for use in engineered tissue includes a repeated 3D geometry; and at least one of a hydrogel, a biocompatible polymer, and a bioprintable polymer. The repeated 3D geometry may include an open geometry including a plurality of interconnected nodes configured to be seeded with living cells. The scaffold may be formed via bioprinting. The three-dimensional scaffold may also be used for (1) 3D tissue modelling (for liver, kidney, gut, pancreas, heart, lung, spleen, etc.), (2) disease modeling of different tumor types such as breast, colorectal, lung, liver, brain, ovarian and others, (3) drug testing / therapeutic screening of small molecule drugs, peptides, proteins, antibodies, ADCs, gene therapies, RNA-based therapeutics, cell therapies, and tissue therapies, and (4) therapeutic / drug delivery (i.e., via delivery device that can be used to control drug release based on different patterns).

The invention provides a preparation method of a cell culture three-dimensional scaffold and cell culture meat, which comprises the following steps: firstly, preparing a gelatin-carrageenan mixed solution and a gel precursor solution of transglutaminase, dividing into two parts, incubating at constant temperature to obtain two parts of composite hydrogel, randomly freeze-drying one part to obtain aerogel, and freeze-drying the aerogel to obtain the cell culture three-dimensional scaffold. Stacking on the other part and incubating at constant temperature again to obtain the prefabricated double-layer gel. The prefabricated double-layer gel is directionally frozen, ice crystals grow upwards from the bottom, freezing is stopped after the temperature is reduced to the set temperature, and the cell culture three-dimensional scaffold is obtained through drying. The stent adopts an ordered-disordered partition design, parallel micro-channels are formed in the lower layer by using a directional ice template method, and muscle fiber arrangement is simulated; a porous spongy structure is constructed on the upper layer by using a random ice template method, and is adapted to adipocyte proliferation. Two layers of interfaces are interpenetrated and communicated, natural mechanical gradients are combined, natural tissue characteristics are re-engraved, the traditional limitation is broken through, the cell co-culture efficiency and the cultured meat quality are improved, and industrialization is promoted.

The invention discloses a preparation method of a functional bone repair hydrogel scaffold, which comprises the following steps: mixing nano attapulgite and methacrylated gelatin to form a printable emulsion system, performing three-dimensional printing on the printable emulsion system, and performing ultraviolet crosslinking to form a porous three-dimensional scaffold, and incubating the porous three-dimensional scaffold in a PBS (Phosphate Buffer Solution) containing the mesenchymal stem cell exosome and the miRNA-26a-5p and / or the miRNA-30a-5p, so that the mesenchymal stem cell exosome, the miRNA-26a-5p and / or the miRNA-30a-5p are co-loaded on the porous three-dimensional scaffold. The invention also provides a scaffold prepared by the method and application of the scaffold in biology. The scaffold can load mesenchymal stem cell exosomes and specific micro-RNA, and realizes regulation and control of immune microenvironment of injured parts and promotion of bone regeneration, so that the repair effect of large-volume bone defects is effectively improved.

The present invention relates to a biofabrication system for bioprinting and / or patterning three dimensional scaffold and retention structures using biofilm producing bacteria as the means of synthesizing geometries. The machine comprises a negative mobile mold of the final three dimensional shape desired and one or more oxygen blocking floaters to allow biofilm growth only in the positive part of the aforementioned mold. The bioprinter movement is calibrated relative to the culture medium surface level to maintain continuous biofilm growth. The bioreactor may be used along with the ability to control temperature, humidity, air flow and culture media conditions and nutrients as the three dimensional synthesis requires.

Novel nanoconjugate compositions, such as nanoparticle-oligomer arrays (NOAs), and related methods for precision therapeutics and molecular diagnostics. These compositions comprise a core, optionally a nanoparticle core, and a plurality of oligomeric strands forming a dense, highly oriented three-dimensional scaffold. This scaffold architecture is engineered to position functional molecules precisely at desired distances from the core and / or the external surface, thereby controlling their physical and chemical interactions with the core and / or microenvironment, enabling distinct functionalities not observed with the functional molecules in an unstructured formulation.

A method is provided. The method includes: generating light by a plasma of a light source of a semiconductor processing tool; generating patterned light by a mask assembly, the patterned light including the light reflected by a pattern of the mask assembly; during generating the patterned light, protecting the mask assembly by a pellicle assembly including a pellicle membrane, the pellicle membrane including a nanotube-based scaffold structure having nanotubes bound together by a capping layer; and performing a semiconductor process on a semiconductor wafer by the patterned light.

The present disclosure provides, among other things, a method of isolating and expanding gamma delta (γδ) T cells, wherein the method comprises: (a) isolating non-hematopoeitic tissue by biopsy or explant, and (b) culturing the isolated non-hematopoeitic tissue in the absence of a three-dimensional scaffold or grid, thereby expanding and isolating gamma delta (γδ) T cells.

The present invention provides a bionic three-dimensional scaffold for promoting the repair of aged bone defects and targeting the aging pathway, and a preparation method thereof, the method comprising the following steps: 1) preparing mesoporous bioactive glass nanospheres; 2) loading anti-aging drugs or inhibitors targeting the aging pathway into the mesoporous bioactive glass nanospheres; 3) blending the drug-loaded nanospheres with polycaprolactone to obtain a nanoscale fiber membrane; 4) cutting the fiber membrane into pieces, freeze-drying to obtain a drug-loaded three-dimensional electrospinning nanoscaffold; 5) placing in a polydopamine solution for surface modification. A bionic three-dimensional scaffold for promoting the repair of aged bone defects and targeting the aging pathway provided by the present invention has an extracellular matrix structure similar to that of natural bone, and the loaded drugs can target the aging mechanism, reduce the burden of aging cells, and increase cell activity, and can be used for the repair of aged bone defects. It can also prevent multiple aging complications at the same time by targeting the basic aging mechanism, providing a direction for future clinical trials.

The present disclosure pertains to a 3D scaffold for cell growth and proliferation. In particular, the present disclosure provides a method of producing an artificial 3D scaffold to support stem cell growth and later their differentiation, by converting biodegradable amphiphilic copolymers (star polymer) into nanowire scaffolds, through a molecular self-assembly process. The invention also relates to the use of said scaffold for cell culture and / or transplantation.

According to the self-energized biological 3D printing bio-ink, the 3D printing bioactive scaffold and the preparation method of the self-energized biological 3D printing bio-ink, the controllable-release self-energized biological 3D printing bio-ink is prepared by mixing the organic high-molecular polymers with different molecular weights and the energy metabolism promoting substances, and the ink is good in biocompatibility; by changing the content of energy supply substances, the ink can be designed to'provide 'and'accept' different cell metabolism regulation clues in an adjustable time regulation manner, better migration and adhesion of cells are realized, and functions are exerted, and meanwhile, the ink has controllable slow release of energy metabolism promoting substances and generates micro-nano pores when being used for constructing a three-dimensional bracket through 3D printing, so that the energy metabolism promoting effect is improved. The hydrophilicity of the scaffold is improved, the biological energy metabolism activity of the composite material is enhanced, the energy metabolism promoting substance can provide a sufficient nutrition environment for cell growth, the cell energy metabolism process is regulated and controlled, and adhesion, growth, proliferation and functionalization of cells are facilitated.

Described herein are three-dimensional scaffold compositions and methods for bone repair or regeneration. In some embodiments, the disclosed scaffolds comprise a core portion mimicking a native trabecular bone structure that is surrounded by an exterior portion mimicking a native cortical bone structure. In some embodiments, the scaffolds may be functionalized by mineralization and / or pre-vascularization to promote bone and blood vessel formation.

A sterilized PLA-collagen scaffold comprising animal collagen and a method for preparing a sterilized scaffold for medical use, the method comprising the steps of:i) Loading animal collagen to a fiber mesh containing fibers of polylactide polymer or copolymer (commonly denoted PLA) to obtain a PLA-collagen scaffold,ii) Drying the PLA-collagen scaffold obtained from step i),iii) Sterilizing the PLA-collagen scaffold obtained from the drying step ii) to obtain the sterilized scaffold.The sterilized scaffold obtained has improved biomechanical and physical properties compared with an unsterilized scaffold.

The application belongs to the technical field of bone tissue engineering, and particularly relates to a recombinant collagen-chitosan-PEG-hydroxyapatite bone repair scaffold material, a preparation method and application. The bone repair scaffold material is formed into a one-way ordered three-dimensional scaffold by a directional freezing method through recombinant collagen, chitosan and PEG. Nano-hydroxyapatite is uniformly deposited in the inside and surface through a special mineralization process. The prepared material has a very good bionic natural bone structure and composition, a good reticular structure and mechanical strength, good biocompatibility and cell activity, a significant repair effect on critical size bone defects, a good osteogenic induction effect and bone conductivity. The bone repair scaffold material provides a new potential treatment method for severe bone defects, and has a wide application in the fields of artificial bone, artificial cartilage, biological scaffolds and the like.

A method of preparing a three-dimensional scaffold comprises obtaining fish scales including a plurality of microgrooves and ridges having a width ranging from about 25 μM to about 30 μM and sterilizing the fish scales to provide the three-dimensional scaffold. The three-dimensional scaffold can be used for in vitro cell growth.

The present invention is directed to a method for ex-vivo-engineering of cells, in particular stem cells or T cells, preferably hematopoictic stem and / or progenitor cells, mesenchymal stem cells, or T cells comprising a step of culturing the cells on a three-dimensional scaffold. The method of the invention is capable of improving the efficiency of genetic modification of cells and the functionality of the engineered cells.

Device (1), and method for manufacturing the same, for detecting the growth of a three-dimensional cell cluster (C), comprising: a substrate (2) having a support surface (21); a plurality of electrodes (3) arranged according to a predetermined spatial distribution, for detecting an electrical potential in the presence of a three-dimensional cell cluster (C); a plurality of three-dimensional scaffolds (4), comprising at least a first scaffold (41), arranged at a respective measuring electrode (31), and at least a second scaffold (42), arranged at a respective reference electrode (32), wherein the at least one first scaffold (41) has a proliferation conformation, adapted to promote the growth of the three-dimensional cell cluster (C), and the at least one second scaffold (42) has an inhibition conformation adapted to prevent the growth of the three- dimensional cell cluster (C).

A three dimensional scaffold for generating cell or protein assemblies. This degradable scaffold can be applied to various types of cells. Also disclosed are methods of treating a condition by implanting the protein or cell assembly prepared according to the method described herein.

The present disclosure relates generally to chemical sensors and polymer coatings for chemical sensors, and more particularly to a sensing system including a sensor having a polymeric 3D printed coating disposed on a surface thereof. A sensing system includes a sensor including a resonator structure. The sensing system includes a polymeric 3D printed coating disposed on a surface of at least a portion of the sensor. The polymeric 3D printed coating includes a three-dimensional scaffold structure.

An in vitro construct useful for toxicity testing is provided, comprising: a three-dimensional (3D) scaffold comprising silk fibroin and having a crosslinked porous matrix; and stem cells adherent to the 3D scaffold. In some embodiments, the stem cells adherent to the 3D scaffold maintain stable mitochondrial DNA in long term culture. In some embodiments, the stem cells are urine stem cells.

The present disclosure relates to a three-dimensional scaffold comprising a plurality of scaffold elements, each scaffold element comprising at least one amino acid peptide component and at least two nucleic acid components, the peptide component consisting of a length of amino acid wherein the at least two nucleic acid component portions of the plurality of scaffold elements are configured to mediate self-assembly of the scaffold.

The invention relates to the technical field of biomedicine, in particular to a mesenchymal stem cell differentiation method for promoting bone joint repair, which comprises the following steps: inoculating mesenchymal stem cells on a three-dimensional scaffold material to form a complex, applying an alternating electric field within a specific parameter range for stimulation, and combining with growth factor culture to realize directional differentiation. According to the method, by regulating and controlling the frequency, the voltage amplitude and the action time of the alternating electric field, the differentiation efficiency of the cells in the cartilage or osteogenesis direction is remarkably improved, and meanwhile the adaptability of the cells in the complex bone joint microenvironment is enhanced.

The invention provides a multifunctional artificial eye seat with polar radiation aperture distribution and a preparation method thereof, and belongs to the technical field of biomedical materials. The artificial eye seat is a porous spherical or ellipsoidal stent taking trace copper doped zinc melilite biological ceramic as a main component. The internal micropores are mutually communicated curved surface pore units, the pore diameters of the micropores are radially expanded from one end of the spherical surface to the opposite side in a macropore-mesopore-micropore gradient layout, a polar radiation pore diameter distribution three-dimensional bracket model is printed by adopting photocuring ceramic, and then the polar radiation pore diameter distribution three-dimensional bracket model is cleaned, dried and sintered to form the polar radiation pore diameter distribution three-dimensional bracket; the artificial eye seat gives consideration to the anatomical physiological structure of the orbit, the surface of the macropore area is attached to the deep soft tissue of the orbit, and the micropore area faces the ocular surface, so that vascularized soft tissue ingrowth is efficiently promoted, infection is durably resisted, common risks such as displacement and exposure of the artificial eye seat are effectively overcome, and the artificial eye seat has good application value in orbit reconstruction and reshaping.

Engineered tissue models based on three-dimensional (3D) scaffolds, also referred to herein as tissue-engineered 3D models, can be used as in vitro diagnostic and drug screening tools for predicting, preventing and / or treating cancer metastases.

Method for the production of extracellular vesicles, EVs, loaded with a molecule of interest in a bioreactor containing a porous three-dimensional, 3D, scaffold, wherein the method comprises a seeding of the scaffold with a starting number of adherent immortalized cells resuspended after detachment, an attachment of the cells on said scaffold, an expansion of the cells on said scaffold up to a second number of adherent cells, defining a multiplication factor of at least 5 for a single expansion step, a production of EVs by said cells, a phase of loading said EVs with said molecule of interest, a harvest of EVs.

This application relates to the field of cell preservation, and discloses a long-term preservation medium for stem cells based on a three-dimensional biomimetic microenvironment, which comprises the following component materials in parts by mass: flexible skeleton, benzoyl peroxide, superoxide dismutase, retinoic acid, ferulic acid, mixed buffer, nucleic acid aptamer targeting stem cells, serum-free medium, bFGF and TGF-β. Its construction method comprises the following steps: S1, three-dimensional scaffold construction; S2, antioxidant loading; S3, buffer preparation; S4, aptamer modification; S5, medium integration. The three-dimensional scaffold structure constructed by natural polysaccharides and nanocomposites, combined with the biomimetic pore and flexible network design, effectively simulates the extracellular matrix microenvironment. At the same time, by improving the structural stability and chemical compatibility of the cell adhesion interface, the cell morphology maintenance and adhesion force maintenance during the freezing process are realized, so that the scaffold provides a dynamically adaptable stable environment for cells structurally.

A microfluidic device includes multiple microfluidic assay units arranged on a substrate, with each assay unit including multiple scaffold regions each containing a three-dimensional scaffold with associated cells, and a media channel surrounding a fluid-permeable boundary portion of at least a second scaffold region, wherein a fluid-permeable interface between the media channel and the second scaffold region comprises a curved shape spanning an arc of more than 90 degrees. A third scaffold region may be provided. Boundaries between different scaffold regions, and between a scaffold region and the media channel, may include microposts that may be spaced apart in a curved configuration. A method for performing an assay utilizing such a device is further provided.

The invention discloses a three-dimensional bracket and a preparation method thereof. The three-dimensional scaffold is of a three-dimensional porous network structure formed by interweaving silicon dioxide nanofibers, and the three-dimensional scaffold is prepared by performing gas foaming treatment on a silicon dioxide nanofiber membrane; an epigallocatechin gallate-metal ion composite coating can be further loaded on the surface of the stent, and metal ions are preferably copper ions. The preparation method comprises the following steps: preparing PVA and TEOS into a spinning solution, and carrying out electrostatic spinning and high-temperature calcination to obtain a silicon dioxide nanofiber membrane; and then soaking in a sodium borohydride solution for gas foaming treatment to construct the three-dimensional porous scaffold. Structural transformation from a two-dimensional membrane to a three-dimensional through porous scaffold is achieved through mild gas foaming, the material is endowed with high water absorption, remarkable antibacterial and antioxidant activity and excellent angiogenesis and osteogenesis promoting performance by combining with the active coating, and the material has good application prospects in the field of tissue regeneration engineering.

A system for collecting biomarkers from a fluid stream includes a collection vessel having at least one fluid port that is adapted to be in fluid communication with the fluid stream. The collection vessel defines an inner volume. A 3D scaffold is disposed within the inner volume of the collection vessel. The 3D scaffold is chemically functionalized to bind the biomarkers in the fluid stream, wherein the biomarkers are eukaryotic cells of interest, extracellular vesicles associated with the eukaryotic cells of interest, or combinations thereof. A fluid driver circulates the fluid stream through the collection vessel via the at least one fluid port such that the fluid stream interacts with the 3D scaffold.

The invention discloses a method for preparing an exosome by simulating an atherosclerosis microenvironment, and relates to the technical field of biology, and the method comprises the following steps: (1) separation and culture of umbilical cord mesenchymal stem cells; (2) establishing a microenvironment for simulating the coronary atherosclerotic heart disease, and culturing in combination with a three-dimensional bracket; (3) extracting an exosome; the three-dimensional scaffold is obtained by taking an acellular matrix, a temperature-sensitive material and polypyrrole nanoparticles as printing materials through low-temperature deposition 3D printing; a composite three-dimensional stent containing an acellular matrix, a temperature-sensitive material Pluronic F127 and polypyrrole nanoparticles is constructed by adopting a low-temperature deposition 3D printing technology, the coronary atherosclerotic heart disease pathological microenvironment is simulated, and the coronary atherosclerotic heart disease is obtained by combining the technical means of dynamic mechanical regulation and electrical stimulation. According to the application, the umbilical cord mesenchymal stem cells are successfully induced to secrete the exosome of which the secretion amount is remarkably increased and the functionality is enhanced.

The invention relates to the technical field of biomedical engineering, and discloses a dynamic culture device and a dynamic culture method.The dynamic culture device comprises a culture tank, culture dishes, a disturbance device, a monitoring module and a control system, the multiple culture dishes with through holes are arranged in the vertical direction, and the disturbance device drives a magnetic rotor to rotate through magnetic force; the monitoring module is used for detecting and adjusting culture environment parameters, and the control system controls operation of the monitoring module and the magnetic driving device. Under the disturbance of the magnetic rotor, the culture medium forms a dynamic flow field and flows through the culture dishes, the flow field penetrates through the through holes of the culture dishes to promote the culture medium to circulate among the culture dishes, so that the mechanical stimulation response of the three-dimensional scaffold containing the cells is enhanced, the secretion activity of the cells is promoted to be activated, and the dynamic flow field can also effectively remove metabolic wastes and improve the activity of the cells. Nutrients are supplemented in real time, so that the production efficiency of cell active materials is improved. The monitoring module and the control system realize controllability of culture environment parameters, and improve stability and consistency of production of cell active materials.