27 results about "Three dimensional scaffolds" patented technology

The invention provides a preparation method of a cell culture three-dimensional scaffold and cell culture meat, which comprises the following steps: firstly, preparing a gelatin-carrageenan mixed solution and a gel precursor solution of transglutaminase, dividing into two parts, incubating at constant temperature to obtain two parts of composite hydrogel, randomly freeze-drying one part to obtain aerogel, and freeze-drying the aerogel to obtain the cell culture three-dimensional scaffold. Stacking on the other part and incubating at constant temperature again to obtain the prefabricated double-layer gel. The prefabricated double-layer gel is directionally frozen, ice crystals grow upwards from the bottom, freezing is stopped after the temperature is reduced to the set temperature, and the cell culture three-dimensional scaffold is obtained through drying. The stent adopts an ordered-disordered partition design, parallel micro-channels are formed in the lower layer by using a directional ice template method, and muscle fiber arrangement is simulated; a porous spongy structure is constructed on the upper layer by using a random ice template method, and is adapted to adipocyte proliferation. Two layers of interfaces are interpenetrated and communicated, natural mechanical gradients are combined, natural tissue characteristics are re-engraved, the traditional limitation is broken through, the cell co-culture efficiency and the cultured meat quality are improved, and industrialization is promoted.

Novel nanoconjugate compositions, such as nanoparticle-oligomer arrays (NOAs), and related methods for precision therapeutics and molecular diagnostics. These compositions comprise a core, optionally a nanoparticle core, and a plurality of oligomeric strands forming a dense, highly oriented three-dimensional scaffold. This scaffold architecture is engineered to position functional molecules precisely at desired distances from the core and / or the external surface, thereby controlling their physical and chemical interactions with the core and / or microenvironment, enabling distinct functionalities not observed with the functional molecules in an unstructured formulation.

A method is provided. The method includes: generating light by a plasma of a light source of a semiconductor processing tool; generating patterned light by a mask assembly, the patterned light including the light reflected by a pattern of the mask assembly; during generating the patterned light, protecting the mask assembly by a pellicle assembly including a pellicle membrane, the pellicle membrane including a nanotube-based scaffold structure having nanotubes bound together by a capping layer; and performing a semiconductor process on a semiconductor wafer by the patterned light.

The present disclosure provides, among other things, a method of isolating and expanding gamma delta (γδ) T cells, wherein the method comprises: (a) isolating non-hematopoeitic tissue by biopsy or explant, and (b) culturing the isolated non-hematopoeitic tissue in the absence of a three-dimensional scaffold or grid, thereby expanding and isolating gamma delta (γδ) T cells.

The present disclosure pertains to a 3D scaffold for cell growth and proliferation. In particular, the present disclosure provides a method of producing an artificial 3D scaffold to support stem cell growth and later their differentiation, by converting biodegradable amphiphilic copolymers (star polymer) into nanowire scaffolds, through a molecular self-assembly process. The invention also relates to the use of said scaffold for cell culture and / or transplantation.

Described herein are three-dimensional scaffold compositions and methods for bone repair or regeneration. In some embodiments, the disclosed scaffolds comprise a core portion mimicking a native trabecular bone structure that is surrounded by an exterior portion mimicking a native cortical bone structure. In some embodiments, the scaffolds may be functionalized by mineralization and / or pre-vascularization to promote bone and blood vessel formation.

A sterilized PLA-collagen scaffold comprising animal collagen and a method for preparing a sterilized scaffold for medical use, the method comprising the steps of:i) Loading animal collagen to a fiber mesh containing fibers of polylactide polymer or copolymer (commonly denoted PLA) to obtain a PLA-collagen scaffold,ii) Drying the PLA-collagen scaffold obtained from step i),iii) Sterilizing the PLA-collagen scaffold obtained from the drying step ii) to obtain the sterilized scaffold.The sterilized scaffold obtained has improved biomechanical and physical properties compared with an unsterilized scaffold.

The application belongs to the technical field of bone tissue engineering, and particularly relates to a recombinant collagen-chitosan-PEG-hydroxyapatite bone repair scaffold material, a preparation method and application. The bone repair scaffold material is formed into a one-way ordered three-dimensional scaffold by a directional freezing method through recombinant collagen, chitosan and PEG. Nano-hydroxyapatite is uniformly deposited in the inside and surface through a special mineralization process. The prepared material has a very good bionic natural bone structure and composition, a good reticular structure and mechanical strength, good biocompatibility and cell activity, a significant repair effect on critical size bone defects, a good osteogenic induction effect and bone conductivity. The bone repair scaffold material provides a new potential treatment method for severe bone defects, and has a wide application in the fields of artificial bone, artificial cartilage, biological scaffolds and the like.

The present invention is directed to a method for ex-vivo-engineering of cells, in particular stem cells or T cells, preferably hematopoictic stem and / or progenitor cells, mesenchymal stem cells, or T cells comprising a step of culturing the cells on a three-dimensional scaffold. The method of the invention is capable of improving the efficiency of genetic modification of cells and the functionality of the engineered cells.

Device (1), and method for manufacturing the same, for detecting the growth of a three-dimensional cell cluster (C), comprising: a substrate (2) having a support surface (21); a plurality of electrodes (3) arranged according to a predetermined spatial distribution, for detecting an electrical potential in the presence of a three-dimensional cell cluster (C); a plurality of three-dimensional scaffolds (4), comprising at least a first scaffold (41), arranged at a respective measuring electrode (31), and at least a second scaffold (42), arranged at a respective reference electrode (32), wherein the at least one first scaffold (41) has a proliferation conformation, adapted to promote the growth of the three-dimensional cell cluster (C), and the at least one second scaffold (42) has an inhibition conformation adapted to prevent the growth of the three- dimensional cell cluster (C).

A three dimensional scaffold for generating cell or protein assemblies. This degradable scaffold can be applied to various types of cells. Also disclosed are methods of treating a condition by implanting the protein or cell assembly prepared according to the method described herein.

The present disclosure relates generally to chemical sensors and polymer coatings for chemical sensors, and more particularly to a sensing system including a sensor having a polymeric 3D printed coating disposed on a surface thereof. A sensing system includes a sensor including a resonator structure. The sensing system includes a polymeric 3D printed coating disposed on a surface of at least a portion of the sensor. The polymeric 3D printed coating includes a three-dimensional scaffold structure.

An in vitro construct useful for toxicity testing is provided, comprising: a three-dimensional (3D) scaffold comprising silk fibroin and having a crosslinked porous matrix; and stem cells adherent to the 3D scaffold. In some embodiments, the stem cells adherent to the 3D scaffold maintain stable mitochondrial DNA in long term culture. In some embodiments, the stem cells are urine stem cells.

The invention provides a multifunctional artificial eye seat with polar radiation aperture distribution and a preparation method thereof, and belongs to the technical field of biomedical materials. The artificial eye seat is a porous spherical or ellipsoidal stent taking trace copper doped zinc melilite biological ceramic as a main component. The internal micropores are mutually communicated curved surface pore units, the pore diameters of the micropores are radially expanded from one end of the spherical surface to the opposite side in a macropore-mesopore-micropore gradient layout, a polar radiation pore diameter distribution three-dimensional bracket model is printed by adopting photocuring ceramic, and then the polar radiation pore diameter distribution three-dimensional bracket model is cleaned, dried and sintered to form the polar radiation pore diameter distribution three-dimensional bracket; the artificial eye seat gives consideration to the anatomical physiological structure of the orbit, the surface of the macropore area is attached to the deep soft tissue of the orbit, and the micropore area faces the ocular surface, so that vascularized soft tissue ingrowth is efficiently promoted, infection is durably resisted, common risks such as displacement and exposure of the artificial eye seat are effectively overcome, and the artificial eye seat has good application value in orbit reconstruction and reshaping.

A microfluidic device includes multiple microfluidic assay units arranged on a substrate, with each assay unit including multiple scaffold regions each containing a three-dimensional scaffold with associated cells, and a media channel surrounding a fluid-permeable boundary portion of at least a second scaffold region, wherein a fluid-permeable interface between the media channel and the second scaffold region comprises a curved shape spanning an arc of more than 90 degrees. A third scaffold region may be provided. Boundaries between different scaffold regions, and between a scaffold region and the media channel, may include microposts that may be spaced apart in a curved configuration. A method for performing an assay utilizing such a device is further provided.

The invention discloses a three-dimensional bracket and a preparation method thereof. The three-dimensional scaffold is of a three-dimensional porous network structure formed by interweaving silicon dioxide nanofibers, and the three-dimensional scaffold is prepared by performing gas foaming treatment on a silicon dioxide nanofiber membrane; an epigallocatechin gallate-metal ion composite coating can be further loaded on the surface of the stent, and metal ions are preferably copper ions. The preparation method comprises the following steps: preparing PVA and TEOS into a spinning solution, and carrying out electrostatic spinning and high-temperature calcination to obtain a silicon dioxide nanofiber membrane; and then soaking in a sodium borohydride solution for gas foaming treatment to construct the three-dimensional porous scaffold. Structural transformation from a two-dimensional membrane to a three-dimensional through porous scaffold is achieved through mild gas foaming, the material is endowed with high water absorption, remarkable antibacterial and antioxidant activity and excellent angiogenesis and osteogenesis promoting performance by combining with the active coating, and the material has good application prospects in the field of tissue regeneration engineering.

A system for collecting biomarkers from a fluid stream includes a collection vessel having at least one fluid port that is adapted to be in fluid communication with the fluid stream. The collection vessel defines an inner volume. A 3D scaffold is disposed within the inner volume of the collection vessel. The 3D scaffold is chemically functionalized to bind the biomarkers in the fluid stream, wherein the biomarkers are eukaryotic cells of interest, extracellular vesicles associated with the eukaryotic cells of interest, or combinations thereof. A fluid driver circulates the fluid stream through the collection vessel via the at least one fluid port such that the fluid stream interacts with the 3D scaffold.

Provided are biospheres comprising: (a) a three-dimensional scaffold matrix for biological growth; (b) a first type of cell configured to be in contact with the three-dimensional scaffold matrix to grow to generate the biosphere with an internal volume; and (c) a second type of cell disposed in the internal volume. Also provided are methods of creating the same.

Synthetic cellular support systems in the form of engineered extracellular matrices are provided. The cellular support system may include a three-dimensional scaffold structure comprising at least one void. At least one suspended fibril spans across the at least one void in the three-dimensional scaffold structure. The suspended fibril comprises at least one extracellular matrix protein, such as fibronectin, and at least one glycan, such as a hyaluronic acid. The suspended fibril is capable of supporting cells and promoting three-dimensional cellular growth. In various aspects, a plurality of suspended fibrils may span the void to form a three-dimensional suspended fibrillar network.

The invention discloses a method and a system for printing a silk fibroin scaffold by using ultrasound, and belongs to the technical field of biological material manufacturing and tissue engineering. The method comprises the following steps: preparing silk fibroin bio-ink, polydopamine-coated barium titanate composite powder and lipid microbubbles; mixing the three components to obtain composite printing ink; and sequentially applying ultrasonic waves to predetermined three-dimensional point positions in a tool loaded with the ink by using a focused ultrasonic transducer, triggering a local cavitation effect under the assistance of microbubbles, inducing silk fibroin to solidify at a fixed point, and accumulating point by point to form a three-dimensional bracket. The invention further provides an ultrasonic printing device for implementing the method. The preparation method avoids the use of a chemical cross-linking agent, and is green and safe; the cavitation threshold value is reduced through microbubbles, and low-energy and high-efficiency solidification is achieved; fine printing of a complex structure is achieved by combining focused ultrasound and three-dimensional motion control. And a piezoelectric function is integrated, so that the prepared stent has good biocompatibility and bioelectricity activity.

This invention discloses a three-dimensional visualization organoid culture device, belonging to the field of organoid culture technology. It includes a control center, which is communicatively connected to a virtual three-dimensional scaffold module, a laser visualization monitoring module, a biolayer interference sensing module, and an automated culture medium control module. This invention abandons the traditional matrix gel solid scaffold and employs focused acoustic vortex technology to construct a dynamically adjustable virtual acoustic trap array. It utilizes acoustic radiation force to achieve cell capture and spatial arrangement, avoiding the interference of uncertainties in the composition and biocompatibility differences of solid scaffolds on organoid growth. Simultaneously, the acoustic trap array can adaptively reconstruct its topology according to the cell aggregation stage and metabolic state, solving the problem that traditional scaffold structures cannot adapt to dynamic cell growth, improving the morphological uniformity and physiological relevance of organoids, and providing a high-quality culture medium for organoid research and clinical applications.

Three-dimensional scaffolds to facilitate engineered tissue growth are described herein. An exemplary scaffold comprises a first capillary element, a second capillary element, and a connective element that spans a distance between the first capillary element and the second capillary element, connecting the capillary elements. Tissues can be grown within the scaffold such that the tissues have highly vascularized structures with many capillaries running throughout. The scaffolds can be fabricated by selective electrochemical etching of a semiconductor element. The electrochemical etching can be controlled by way of a laser configured to stimulate multiphoton absorption in the semiconductor.

The invention belongs to the technical field of bone tissue engineering and regenerative medicine, and particularly discloses a piezoelectric-photo-thermal dual-mode composite stent for articular cartilage repair and a preparation method of the piezoelectric-photo-thermal dual-mode composite stent. The preparation method comprises the three steps of MXene preparation, electrostatic spinning and paper folding structure construction, poly-L-lactic acid and MXene nanosheets are compounded to prepare a fiber membrane, and then the fiber membrane is subjected to hot pressing through a corrugated mold to form the three-dimensional stent with the periodic creased structure. The scaffold has piezoelectric property and photo-thermal property at the same time, promotes cartilage differentiation through the piezoelectric effect, inhibits subchondral bone angiogenesis through the photo-thermal effect, maintains a low-oxygen microenvironment, realizes integral repair of cartilage-subchondral bone functional units, can be used for treatment of cartilage defects, osteochondral defects and osteoarthritis, and has a wide application prospect. The problem that the cartilage steady state is damaged due to the fact that a traditional piezoelectric material promotes bone vascularization is solved.

The application discloses a preparation method of a tissue engineering scaffold for rapidly inducing stem cell proliferation and differentiation and application of the scaffold. The scaffold is prepared as follows: natural high polymer and a sacrificing agent are respectively dissolved in an organic solvent to prepare spinning liquid, two kinds of spinning liquid are mixed, and electrostatic spinning is carried out to obtain a two-dimensional scale tropotaxis fiber scaffold; the two-dimensional fiber scaffold is reacted with a foaming agent solvent to obtain a three-dimensional scaffold with a tropotaxis structure and different elastic moduli. The three-dimensional scaffold with the tropotaxis structure and the different elastic moduli can be obtained by controlling preparation conditions. Stem cells are planted in the three-dimensional scaffold, the expression of key genes PIP2, ALP and Vav is regulated by directly regulating the elastic modulus or further regulating the expression of BCL-6 and MiR-126-5p, stem cell efficient proliferation and differentiation in a heterotropic space are induced, and the scaffold treated by the cells is planted on a damaged wound surface to accelerate wound healing.

The invention discloses a three-dimensional printing photo-thermal immune scaffold for postoperative treatment and repair of skin melanoma and a preparation method of the three-dimensional printing photo-thermal immune scaffold. The preparation method comprises the following steps: S1, preparing manganese silicate nanospheres loaded with an immunologic adjuvant; and S2, mixing the composite nanoparticles obtained in the step S1 with a photo-crosslinking hydrogel precursor solution, a calcium ion source and a photoinitiator to obtain an internal phase slurry, and preparing a sodium alginate aqueous solution as an external phase slurry. According to the invention, a photo-thermal therapeutic agent (manganese silicate), an immunologic adjuvant (imiquimod) and a tissue regeneration promoting factor (manganese and silicon ions) are integrated on a three-dimensional scaffold platform for the first time, and through a precise preparation method, space-time synergy and function integration of local ablation-system immunity-tissue repair are realized.

This invention relates to a three-dimensional scaffold based on chirality and peptides for selective cell adhesion, its preparation method, and its applications, relating to the field of medical products. The three-dimensional scaffold is a stacked, three-dimensional ordered structure, with each layer comprising several ordered polyelectrolyte multilayer membrane soft carriers arranged at an angle between adjacent layers. Each layer of the polyelectrolyte multilayer membrane is formed by alternating deposition of positively charged chiral molecules grafted with specific recognition groups and negatively charged supramolecular polyelectrolytes on the soft carriers, followed by modification with peptides. The two peptides in adjacent polyelectrolyte multilayer membranes are different and are used to promote the specific adhesion of different cells. The three-dimensional scaffold provided by this invention has specific adhesion properties, thereby avoiding excessive cell proliferation in incorrect locations and helping to prevent problems such as re-injury and stenosis of artificial blood vessels.

A method of producing extracellular vesicles comprising: supplying a culture medium to a bioreactor comprising a porous three-dimensional scaffold having a specific surface area and a total volume; inoculating the desorbed and resuspended adherent cells to a stent with the total volume of the stent; attaching the adherent cells which are resuspended after desorption to the scaffold; carrying out amplification on the adherent cells on the bracket; the adherent cells generate extracellular vesicles in a production stage; and harvesting the extracellular vesicles. Wherein the porous three-dimensional scaffold is a porous three-dimensional printing scaffold, the ratio of the specific surface area to the total volume of the porous three-dimensional printing scaffold is 25-250, and the adherent cells amplified on the scaffold are immortalized cells derived from an immortalized cell line. The amplification multiple of the immortalized cells during a single amplification step in the scaffold is at least 5.