50 results about "Membrane function" patented technology

A nutritional supplement composition for normalizing impaired or deteriorating neurological function in humans is composed of: at least one agent which promotes synthesis of ATP and / or creatine phosphate in the body, at least one antioxidant for scavenging free radicals in at least one pathway in the body; at least one agent for normalizing or maintaining membrane function and structure in the body; at least one agent for normalizing or maintaining normal neurotransmitter function in the body; at least one agent for down-regulating cortisol action; and at least one agent for suppressing activation of apoptotic pathways in the body. The composition may further contain one or more of: at least one agent for suppressing inflammation in the body; at least one agent for normalizing or maintaining vascular wall function and structure in the body; at least one agent for normalizing or maintaining function of nerve growth factors and / or neurotropic factors in the body; at least one agent for suppressing toxic metal ionic effects; at least one agent for normalizing or maintaining methyl metabolism in the body; at least one agent for normalizing or maintaining metabolism of insulin and glucose in the body; and at least one agent for up-regulating activity of heat shock proteins in the body. A method for normalizing impaired neurological function in humans modulating nutrient partitioning in a human involves administering the aforementioned composition to the human, preferably on a daily basis, for a therapeutically effective period of time. Preferably, the method further involves having the human follow a stress reduction program, and / or a cognitive retraining program, and / or a dietary program designed to maximize insulin and glucose metabolism.

One or more diaphragm valves are integrated as part of a cover of a microfluidic cassette cartridge. A rigid body portion includes one or more recessed valve areas and a plurality of fluid channels. A recessed valve area is disposed along either a bottom surface or a top surface of the rigid body portion. An inlet fluid channel and an outlet fluid channel are formed within the rigid body portion of each recessed valve area. A flexible membrane cover is sealed over the bottom surface of the body portion, and another flexible membrane cover is sealed over the top surface. A portion of the membrane cover that covers a recessed valve area functions as a valve membrane of the diaphragm valve. The flexible membrane functions as both a cover of the cartridge, thereby sealing and enclosing the appropriate channels, and a valve membrane of the diaphragm valve.

A peritoneal function testing apparatus tests the peritoneal function of a dialysis patient easily and with high accuracy. Standards of four kinetic parameters (CCr, Kt / V, MTACu, and MTACc) available in a definite dialysis guideline are computed in accordance with a relational expression, and a curve showing the relation between MTACu / c and the drained fluid volume is indicated in a graph together with the PET data of the patient which has been prepared separately. Thus, the peritoneal function can be evaluated based on the relative position of the patient's data and the curve showing the standard values in the graph.

Embodiments of the invention are related to anion exchange membranes used in electrochemical metal-air cells in which the membranes function as the electrolyte material, or are used in conjunction with electrolytes such as ionic liquid electrolytes.

The invention relates to the field of biomedicine, in particular to production of a novel human cell-penetrating peptide hPP10 (human Pancreatic Polypeptide) and a transfection method for a mediated plasmid DNA (Deoxyribose Nucleic Acid) of the hPP10. The hPP10 is derived from human cell nucleoproteins; and one segment of polypeptide sequence of a lysine specific demethylated enzyme 4A has a cell penetrating function, can be used for carrying proteins, allows nucleic acids (DNA plasmids) to enter a plurality of types of cells in a transmembrane manner, and is a transmembrane transport carrier with an extremely good development prospect for bioactive molecules such as proteins, nucleic acids and the like.

Disclosed are a novel bionic hepatic tissue engineering scaffold and a interface, and producing method thereof, comprising designing a hepatic tissue engineering scaffold imitating natural hepatic lobule structure, and arranging two separate sets of micro duct structure and a interface thereof for hepatocytes and endothelial cells inside the scaffold. The micro duct interface of endothelial cells can be connected with blood vessels in the nature liver, or with the circulatory system of a bioreactor. The hepatocyte micro duct structure is used for accelerating hepatocyte diffusion when perfusing hepatocytes. A mono-layer flow passage and a interface are produced by press print method, and then pore space between two micro duct structures is formed by freeze drying, so that the porosity factor of the material is increased to make the material have certain semi-permeable membrane function. The three-dimensional scaffold and the interface thereof with hepatocyte micro duct structure and endothelial cell micro duct structure are produced by lamination bonding process.

The invention discloses an expression structure method for constructing Glial cell line-Derived Neurotrophic Factor (GDNF) fusing penetrating peptides, the purification of an expressed protein product and the significance thereof, which is mainly characterized in that (1) GDNF parent is a human source mature type; (2) the N-tail end of the GDNF is fused with PEP-1 penetrating peptides comprising the 21 amino acid; and the expression product of the constructed penetrating peptides GDNF fusing the gene expression plasmid has the membrane penetrating function and biological function of GDNF and can be clinically applied in the treatment of the related diseases.

The invention discloses a GO (graphene oxide) and g-C3N4 composite modified membrane material. A preparation method includes steps: activating an original membrane to recover original membrane flux; adding a surfactant into GO and g-C3N4 mixed dispersion liquid to obtain mixed liquid; soaking the activated original membrane into the mixed liquid to realize ultrafiltration adsorption, and soaking the membrane into aniline hydrochloric acid solution; taking out after soaking is finished, and soaking in hydrochloric acid solution of ammonium persulfate immediately to enable interface polymerization reaction; finally, drying the membrane to obtain the composite modified membrane material. By an ultrafiltration adsorption and interface polymerization combined modification method, GO and g-C3N4are combined for surface composite modification of the membrane material, and a visible light responsive catalytic modified membrane is developed. The novel modified membrane is low in preparation cost and recyclable, pollution resistance is remarkably improved, firmness in modifying agent and photocatalyst loading is achieved, the visible catalytic performance and hydrophilic performance are remarkably enhanced, the forbidden bandwidth of a membrane function layer is decreased, the problem of secondary pollution is avoided, and a promising application prospect is achieved.

The invention discloses a liquid membrane extraction method by taking a hollow fiber composite membrane as a liquid membrane carrier. According to the liquid membrane technology, the hollow fiber composite membrane serves as the liquid membrane carrier, an extract phase is filled and attached to the gap and surface of the hollow fiber composite membrane to form the liquid membrane, a feed liquid phase solution and a reverse extraction phase respectively flow through two sides of the carrier, and the separation effect of in-step extraction and reverse extraction is realized through the liquid membrane function. The hollow fiber composite membrane consists of a braided tube layer and a polymer membrane layer; and meanwhile, the extraction phase and the feed liquid phase are fully mixed before entering the membrane by utilizing a mixer, the dispersing effect of the extraction phase liquid drops in the feed liquid phase is improved, the contact probability of the liquid membrane and the feed liquid solution is enhanced, and the mass transfer efficiency is improved. The imbalanced extraction realized by the liquid membrane technology is utilized, the problems that the liquid membrane attachment amount is small, the liquid membrane is instable, the mass transfer efficiency is low and the like in the hollow fiber supported liquid membrane and the liquid membrane updating technology are solved, the mass transfer coefficient and liquid membrane flux are improved, and the magnitude order of the mass transfer coefficient is 10-5.

The invention belongs to the field of membrane separation and relates to a ceramic base carbon nano tube inorganic composite membrane and a preparation method thereof. According to the invention, a carbon nano tube is used as a membrane function layer, porous ceramic is taken as a base body, superpolymer is taken as a reinforcement material, and a method of coating, dipping and reinforcing, and high temperature carbonizing is adopted, so that the ceramic based carbon nano tube inorganic composite membrane of which the pore diameter is controllable and the porosity factor is 70 to 90 percent is obtained. The ceramic base carbon nano tube inorganic composite membrane has excellent adsorptive property and electric conductivity, and has high membrane permeability and selectivity. According to the invention, loss of porosity factor during the traditional membrane preparation process can be avoided, the mechanical property of the membrane is enhanced, and the preparation method has the advantages that the preparation technology is simple and mass production is facilitated.

The invention relates to a pesticide residue degradation agent for modern Chinese medicines and a preparation method thereof. A micro-nano biotechnology, an enzyme active substance control technology and the like are utilized for the produced pesticide residue degradation agent for the modern Chinese medicines. The plant cell membrane function can be improved by enzyme action and biological decomposition action after the agent is used; an intelligent, sustained and auxiliary agent is also added to improve and change the plant quality; pesticide and heavy metal residues are degraded; the traditional Chinese medicines are strengthened; and agricultural products can be facilitated to achieving the international standard. The pesticide residue degradation agent for the modern Chinese medicines, disclosed by the invention, is prepared from the following materials in parts by weight of: 300-350kg of a pig pancreas extract, 100-150kg of styrene acrylic amino acid concentrate, 50-100kg of plant humic acid, 30-70kg of bone glue, 20-50kg of boric acid, 50-100kg of smoke alkali liquor, 50-200kg of ethanol, 170-500kg of water, 5-10kg of vanillic aldehyde, 1-5kg of compound sodium nitrate powder liquid, 50-150kg of urea, 40-120kg of pyroligneous, 5-10kg of flavones, 5-10kg of ferrous sulfate, and 1-5kg of citric acid by using a certain preparation method.

The invention discloses a restructuring protein with phenotypic knock-up effect for chemotactic factor acceptor CXCR4 and constructing method, which is characterized by the following: proceeding twice genetic reform for restructuring muton SDF-1 / 54R of SDF-1 alpha with CXCR4 antagonistic active; constructing the restructuring protein 54R / Tat / KDEL with SDF-1 / 54R, high effective wearing film carrier Tat(47-57) and endoplasmic reticulum locating fragment KDEL; utilizing high effective wearing film function of Tat(47-57) and endoplasmic reticulum locating function of KDEL; leading CXCR4 specific antagonist SDF-1 / 54R into breast cancer cell; reaching the goal of phenotype knock up film surface CXCR4; laying necessary work foundation for blocking-up breast cancer transition.

The invention belongs to the field of a plant cell engineering technology and molecular biology and relates to a method for building a Chinese cabbage genetic transformation system by cell-penetrating peptide and microspore culture. The method comprises carrying out in-vitro synthesis of a transfection complex by film-penetrating functions of cell-penetrating peptide, adding the transfection complex into a separated microspore suspension liquid, preparing a regeneration plant by a free microspore culture technology and carrying out identification on the regeneration plant. The method builds the Chinese cabbage genetic transformation system based on free microspore culture, provides a study platform for Chinese cabbage genetic breeding and lays the method foundation of Chinese cabbage functional genomics.

Antibacterial small molecule compounds, termed liptins, bind to phosphatidylglycerol in bacterial plasma membranes. The small molecule compounds comprise a three-dimensional complementary binding pocket for phosphatidylglycerol, disrupting membrane function in a bacteriostatic or bactericidal manner. Methods of inhibiting bacterial growth and / or treating Gram-positive or Gram-negative bacterial infection using such compounds are also disclosed.

The invention provides a method for detecting the injury of the mitochondria of the sperms of fishes, which relates to an aquaculture technology and needs to provide a method for detecting the injury of the mitochondria of the sperms of fishes. In the invention, the sperms of the fishes with a certain number are collected. The method is characterized by comprising the following steps of: after washing a sperm specimen by using PBS, centrifuging by 300*g for 5min; and suspending by the PBS, precipitating the sperms, regulating the density of the sperms to be 1*106 / ml, adding Rhl23, hatching for 10min in a water bath with the temperature of 37 DEG C, centrifuging, removing supernatant, washing by the PBS repeatedly, centrifuging, suspending by the PBS, adding PI and dyeing for 5min. The sperms with the normal mitochondria and the injured mitochondria are dyed by adopting a fluorescent double-dyeing method and are directly detected by using a flow type cell analyzer after being preprocessed, and the sperms with favorable mitochondrial membrane functions, the sperms with lost mitochondrial membrane functions and the sperms with necrosis can be quantitatively distinguished.

The invention relates to a method for extracting helium from natural gas through a membrane process. The method is used for extracting helium in natural gas and comprises the following steps: carryingout dehydrogenation pretreatment on feed gas by using a first dehydrogenator to obtain first gas; enabling the first gas to sequentially pass through multiple stages of permeable membranes, and sequentially performing helium separation on the first gas by using the multiple stages of permeable membranes to obtain crude helium; conveying the crude helium gas to a second dehydrogenator, and carrying out dehydrogenation post-treatment on the crude helium gas by using the second dehydrogenator to obtain second gas; and purifying the second gas to obtain helium. According to the method for extracting helium from natural gas through the membrane process, the permeable membranes are adopted, partial pressure differences of gas on the two sides of the permeable membranes function as driving force, and the purpose of gas separation is achieved through transfer rate differences, generated through the steps of dissolution, diffusion, analysis and the like, of components. Compared with a mode ofextracting helium by adopting a low-temperature condensation method in related technologies, the method provided by the invention does not need a low-temperature environment and can be carried out atnormal temperature, and therefore, energy consumption is effectively reduced.

The invention discloses a protein carrier for protein transduction and a preparation method and application thereof. The protein carrier adopts genetic engineering means to perform fusion expression on maltose binding protein (MBP) having foreign protein solubleness for promoting fusion expression, cholera toxin B subunit (CTB) which is non-toxic but has a transmembrane function and an enhanced green fluorescent protein (EGFP), to obtain an oligomeric protein (MCTB-EGFP) which is non-toxic, has soluble expression and has a transmembrane effect. The EGFP is replaced with other proteins, and a foreign protein can enter a cell through common incubation with the cell, so that various mechanisms within the cell are studied and related cell therapy is carried out.

A pervaporation element includes a ceramic monolith having an array of parallel channels separated by porous channel walls extending along an axial length of the monolith, and a functional membrane coating a first plurality of the porous channel walls along the axial length of the monolith. The functional membrane functions to separate a fluid into a retentate portion and a permeate portion. The porous channel walls coated by the functional membrane define a plurality of discrete through segments, where each of the discrete through segments are separated from one another by a plurality of uncoated porous channel walls. Fluid entering the discrete through segments is separated into a retentate portion that exits in substantial portion through the discrete through segments and a permeate portion that exits the ceramic monolith radially outward through the uncoated porous channel walls and through a skin of the monolith.

The invention discloses an SPR biosensing system for detecting a CTC. The SPR biosensing system includes an SPR biosensor, a cell membrane functionalized nanoparticle and a folate modified nanoparticle; the SPR biosensor includes an immunochip having a surface modified by a JUP antibody; the cell membrane functionalized nanoparticle contains a JUP protein and a folate receptor; the cell membrane functionalized nanoparticle is combined with the immunochip via an interaction of an antigen-antibody; and the folate modified nanoparticle is combined with the cell membrane functionalized nanoparticle via an interaction of a folate-folate receptor. According to the SPR biosensing system provided by the invention, the CTC is combined at dual selectivity by using the cell membrane functionalized nanoparticle and the folate modified nanoparticle, a false positive problem caused by single antigen-antibody specificity identification is improved, the detection cost is low, and the operation is convenient.

New use of a cryptoid crab antimicrobial peptide Scyreprocin. The antibacterial protein is derived from a cryptoid crab, and is expressed and purified by using a genetic engineered technology. It is first disclosed that the antimicrobial peptide Scyreprocin not only has good antimicrobial activity, but also has a membrane-penetrating function, has an inhibitory and killing activity against various cancer cells, and at the same time has no cytotoxicity against human non-cancer cells. It has demonstrated that the antibacterial polypeptide has a high efficiency and specific anti-cancer activity, shows great application value, and has a good application prospect in research, development and application as an anti-tumor pharmaceutical ingredient. Provided is new use of the cryptoid crab antibacterial peptide Scyreprocin, which broadens the application range of Scyreprocin, and provides more possibilities for the application of Scyreprocin.

Provided is a separation membrane element whereby permeation performance and durability can be enhanced without compromising the characteristics of a separation membrane having excellent separation membrane capability. This separation membrane element (1) is characterized in that two separation membranes (2, 2) form a pair, the permeation sides thereof facing each other with a gap (5) therebetween, a seal part (6) on the peripheral edge of the gap (5) is sealed, and the separation membranes (2, 2) are connected to each other by two or more resin parts (7) located on the inside relative to the seal part (6) on the peripheral edge.

The invention relates to the technical field of medical formulas, in particular to mouthwash for inhibiting the activity of dental plaque mycoderm. The mouthwash is prepared from the following raw materials in parts by weight: propolis alcohol extract, beta-cyclodextrin, tea polyphenol, peppermint essence, nano-silver particles, gold nano-rod adsorbate, vitamin D, malic acid, tartaric acid, sulfated polysaccharide, gelatin, a surfactant, dichlorobenzene biguanide hexane, radix sophorae flavescentis superfine powder and purified water. Aiming at the fact that the streptococcus mutans surface protein sample with the largest number in the dental plaque participates in adhesion to form a biological membrane, by interfering synthesis of streptococcus mutans cell membrane functional protein, permeation of medicine to the biological membrane is promoted, the drug resistance of thalli to dichlorobenzene biguanide hexane is reduced, and the activity of the dental plaque biological membrane is remarkably reduced.

The invention provides a regulation and control method of a quantum well and quantum dot material energy band structure. The regulation and control method comprises the following steps: step 1, sequentially growing a sacrificial layer, a stress introduction layer and a quantum structure film functional layer on a substrate by using a vacuum deposition method; step 2, etching the stress introduction layer and the quantum structure film function layer to the sacrificial layer by using wet etching or dry etching through photoetching an opening area of the sacrificial layer; step 3, corroding a part of the sacrificial layer through the opening area by using a chemical method, releasing the stress introduction layer and the quantum structure film functional layer, and forming a curled structure or a folded structure by the stress introduction layer and the quantum structure film functional layer through prestress release; and step 4, preserving the device with the curled structure or the folded structure by using a supercritical drying method. The quantum structure film functional layer is made of a quantum well material or a quantum dot material, and in the step 3, different curled structures or folded structures are obtained through different corrosion parameters.

The invention discloses a planting method of a selenium-enriched Luhe green plum. The planting method comprises the steps: uniformly mixing miscellaneous manure or composted human and animal manure, phosphorus powder, lime and surface soil, and carrying out refilling to prepare a planting substrate; and then, selecting plants, and carrying out field planting and water and fertilizer management. Manure such as foliage top-dressing and calcium is replenished during the growth period of the Luhe green plum, so that green plum plants are promoted to be heightened, the quality and amount of fresh roots are improved, it is beneficial to the growth and development, and the economic benefit of green plum production can be obviously increased; an artificial chemical fertilizer is replaced with a natural nutrient substance contained in an extract, and the natural nutrient substance is beneficial to the absorption of the plants and has no hazards to the environment; and the effect of a cell for removing superoxide radicals is improved, the plasma membrane function is protected, and fruit senescence is delayed. Therefore, a high-quality selenium-enriched Luhe green plum product is obtained.

A functional fixer (paster / sheet / membrane) of an indwelling needle / indwelling tube comprises an edge-reserved film 1, a functional water absorption pad 3, a fixing pasting film and a supporting layer 5. The fixing pasting film is made of degradable biology base materials; medical silica gel capable of realizing repeated pasting is used as pasting materials of an adhesive strip; the pasting materials are arrayed in a strip mode or in a dot matrix mode so that the contact area between the pasting materials and the skin can be decreased and breathability can be improved on the premise that pasting strength is guaranteed. The portion, making contact with the skin, of the functional water absorption pad is provided with an anti-adhesion film; a middle layer is a water absorption layer; the water absorption layer is coated with a functional layer which is made of photobiotin composite medical materials or far infrared materials. The radiation effect of the functional layer is utilized to act on the molecular structure of liquid medicine and blood so that molecular groups of the liquid medicine and blood can become small. Through the radiation effect on organism issue on the periphery of an opening punctured by the needle, blood circulation is improved, and pain is alleviated.

DHCP (4,5-dihydroxy-2-cyclopenten-1-one) has been previously shown to have antibacterial activity against Escherichia coli. Global transcriptional pattern of E. coli was analyzed in response to DHCP by DNA microarray. It is now shown that DHCP has widespread effects in E. coli, affecting genes encoding proteins involved in general metabolism and cell membrane synthesis and functions. In addition, rpoS and RpoS-regulated genes responding to various stresses are upregulated. DHCP is also shown to inhibit AI-2, an autoinducer involved in interspecies quorum-sensing, and the genes comprising quorum-regulated processes such as virulence, motility and outer membrane functions are downregulated by DHCP treatment. In addition, cysK which is a known quorum-sensing gene working in an alternate pathway(s) in E. coli increases considerably in response to DHCP. These results suggest that DHCP regulates the switching on / off of the different quorum-sensing circuits in E. coli.

The invention discloses a preparation method of a functional silk fibroin film which is beneficial to cell adhesion. The steps include: transfecting Escherichia coli with a prokaryotic system expression vector carrying (‑RGD‑)4 polypeptide or (‑RGD‑)8 polypeptide gene Cells, induced and cultured for several hours; centrifuge the cell culture liquid, collect and crush the bacterial cells, purify the crushed protein supernatant, collect by enzymatic digestion in one step, and freeze-dry to obtain (‑RGD‑)4 polypeptide powder or (‑RGD ‑)8 Polypeptide powder; degumming, dissolving, dialysis, filtering and concentrating silkworm silk or silkworm cocoons, pouring them into a container with a certain area and air-drying to obtain silkworm fibroin film; immersing the silkworm silk film in excess cross-linking agent solution Then add (‑RGD‑)4 polypeptide or (‑RGD‑)8 polypeptide and react overnight at 4°C to obtain a functional silk fibroin film. The functional silk fibroin membrane prepared by the invention has excellent cell adhesion performance, belongs to biomedical materials, has no cytotoxicity, has recognition sites of cell adhesion proteins, and is beneficial to cell adhesion, growth, and promotion of defect tissue healing.