23 results about "Membrane function" patented technology

The invention provides a sunlight-excited multi-ROS (reactive oxygen species) response oxygen sensitization type hydrogel. The sunlight-excited multi-ROS response oxygen sensitization type hydrogel is prepared from a plant-derived nano capsule-like body (NT), an AQPP liposome (AL) and pluronic F127 serving as a hydrogel matrix. The invention also provides a preparation method of the hydrogel and application of the hydrogel in preparation of medicines for treating skin ulcers. The NTALG causes bacterial membrane function damage after illumination, has a strong antibacterial effect and can promote healing of diabetic wounds.

The application discloses a preparation method of a novel catalyst coated membrane (CCM) functional layer, and belongs to the technical field of membrane electrode assemblies (MEA) of proton exchange membrane fuel cells (PEMFC). 2 The SiO2 with a nominal BET of 150 m 2 / g and a 20 wt% Nafion solution are configured into a solution, and the solvent for the solution is a mixture of isopropyl alcohol and water, wherein the mass ratio of Nafion to oxide is 1; S2, the slurry is subjected to 14 h ball milling and 30 min ultrasonic removal of agglomeration; S3, the SiO2 / Nafion slurry is sprayed onto the PEM cathode / anode surface by using an ultrasonic spraying instrument; S4, the catalyst slurry with an I / C ratio of 0.6 is sprayed onto the PEM cathode / anode surface to which the reinforced functional layer has been added by using the ultrasonic spraying instrument; and S5, the conventional MPL, GDL, sealing ring, flow channel, bipolar plate, insulating plate and clamp are pressed and assembled, and no additional sealing is required. By adding the functional layer, the water retention capacity of the membrane electrode is significantly improved, so that the membrane electrode can still maintain excellent proton conduction and output high power under low-humidity working conditions.

The invention discloses a sebum-imitated recombinant collagen anti-oxidation anti-aging brightening composition and a preparation method thereof, the sebum-imitated recombinant collagen anti-oxidation anti-aging brightening composition is of a core-shell double-phase structure, and the sebum-imitated recombinant collagen anti-oxidation anti-aging brightening composition comprises an aqueous phase core, an aqueous phase core and a composite material, the active ingredient of the aqueous phase core is a covalent bond of carboxymethylated recombinant human collagen III and eclipta oligopeptide; the oil-phase layer wraps the water-phase core and comprises a sebum-imitating three-phase film as well as an antioxidant active matter and a slow-release carrier which are dispersed in the sebum-imitating three-phase film; wherein the volume ratio of the water-phase core to the oil-phase layer is 1: 2-1: 4, a core-shell double-phase structure with the carboxymethylated recombinant collagen-eclipta oligopeptide covalent bond as the water-phase core and the sebum-imitating three-phase membrane functional carrier as the oil-phase layer is constructed, and the limitation that active ingredients in a traditional formula are simply mixed is broken through. According to the structure, the stability of the water phase core is ensured through a chemical covalent bond, and the percutaneous permeability and biocompatibility of the composition are remarkably improved by utilizing the high similarity of a sebum-imitating film and skin lipid.

The invention relates to a spider neurotoxin tail peptide with a cell transmembrane function and application of the spider neurotoxin tail peptide. The amino acid sequence of the spider neurotoxin tail peptide with the cell penetrating function is as shown in SEQ ID NO: 2. The invention also provides application of the cell-penetrating peptide in preparation of a carrier which is used for delivering bioactive molecules and can penetrate cell membranes. The invention further provides a fusion protein composed of the spider neurotoxin tail peptide and spider neurotoxin omega-Pp1b and application of the fusion protein in preparation of insecticides. The invention also provides an amphiphilic peptide and application thereof in preparation of a carrier which is used for delivering bioactive molecules and can penetrate through a cell membrane. The invention also provides a nano-composite and application of the nano-composite in preparation of a preparation for insect cell gene silencing. The invention also provides a pesticide composition. The invention is a novel cell-penetrating peptide derived from an insect system, and has important significance for promoting the development of peptide biological insecticides and RNA pesticides.

The application discloses an activatable cell-penetrating peptide, a preparation method, a drug delivery method and an application thereof. The activatable cell-penetrating peptide comprises polypeptide segment 1 and polypeptide segment 2; the polypeptide segment 1 comprises a peptide chain 1, the N terminal of the peptide chain 1 is acetylated and connected with a cyclic peptide RGD, and a group with a cyano group is further connected to the peptide chain 1; the polypeptide segment 2 comprises a peptide chain 2, the N terminal of the peptide chain 2 is also connected with a cyclic peptide RGD, and the peptide chain 2 contains cysteine. The application designs a novel strategy of in-situ activatable cell-penetrating peptide based on an activation nitrile-amino thiol coupling reaction, and the cell-penetrating peptide is composed of two independent low cell-penetrating activity polyarginine polypeptides; after reaching a target site, the two are coupled in-situ through the above-mentioned click chemistry reaction to generate an octa-arginine sequence with high cell-penetrating ability. The design of the 'on-demand activation' not only restores the cell-penetrating function, but also significantly reduces the cytotoxicity caused by non-specific penetration.

The invention provides a method for functional reconstruction of a regenerated membrane, and belongs to the technical field of recycling of waste membrane materials. The method comprises the following steps: carrying out Schiff base reaction on a regenerated membrane and an activator solution to obtain a pre-activated membrane; and reacting the pre-activated membrane with a polyphenol modifier solution to obtain the functionalized regenerated membrane. The water flux of the obtained functionalized regenerated membrane is high and reaches up to 180 L m <-2 > h <-1 > bar <-1 > or above, and the rejection rate gt of organic pollutants such as protein, grease and polysaccharide is high; when the flux is continuously operated for 48 hours, the irreversible attenuation rate of the flux is 1t; and the anti-pollution performance is excellent.

A small activating RNA (saRNA) capable of activating a CCAAT enhancer binding protein alpha (CEBPA) gene, and a delivery system and use thereof are provided, where sense and antisense strands of the saRNA have nucleotide sequences set forth in SEQ ID NO: 1 to SEQ ID NO: 2, respectively. A biomimetic nano-delivery system for targeted delivery of the saRNA is a biomimetic nanoparticle formed by composite nanoparticle coated by a biomembrane of an inflammatory effector cell, where the composite nanoparticle is formed by histone loading with the saRNA capable of activating the CEBPA gene. The biomimetic nanoparticle can inherit antigens and related membrane functions of the inflammatory effector cell and specifically accumulate at the sites of inflammatory lesions, which enables the biomimetic nanoparticle to not only have a longer circulation time in vivo, but also show inflammatory tropism.

The invention relates to the field of biological materials, in particular to a preparation method of a bioprosthetic valve, the bioprosthetic valve is obtained by wrapping a cross-linked extracellular matrix material with a non-cross-linked biological basement membrane to form a sandwich structure, and the preparation method specifically comprises the following steps: respectively preparing the non-cross-linked biological basement membrane and the cross-linked extracellular matrix material; and the collagen derivative mixed solution with the area concentration of 8-50 [mu] L / cm < 2 > and the activator mixed solution with the area concentration of 1-10 [mu] L / cm < 2 > are combined and laminated. The advantages of good regeneration and repair performance and carrying of rich bioactive factors of the basement membrane are utilized, growth and adhesion of endothelial cells on the surface of the material are promoted, an endothelial structure similar to natural blood vessels is formed, the composite bioprosthetic valve has good anti-calcification performance, adhesion of platelets and blood cells is reduced, and the bioprosthetic valve has good anti-calcification performance. The recovery and reconstruction of the valve function are accelerated; moreover, erythrocyte rupture and dissolution are not easily caused, the damage degree to erythrocytes is low, and the blood coagulation risk is low.

The present application relates to the field of biomaterials, and particularly relates to a biomimetic biological valve. The biomimetic biological valve is obtained by wrapping a non-crosslinked biological basement membrane around a crosslinked extracellular matrix material to form a sandwich structure. The basement membrane cooperates with the crosslinked extracellular matrix material, fully plays the excellent regenerative repair performance and the characteristics of being rich in bioactive factors of the basement membrane, effectively promotes the growth and adhesion of endothelial cells on the surface of the material, and constructs a tissue structure similar to the natural valve leaflet. The biomimetic biological valve exhibits excellent anti-calcification performance and excellent dynamic mechanical stability and compliance, significantly inhibits the abnormal adhesion of platelets and blood cells, greatly improves the recovery and reconstruction efficiency of the valve function, and can maintain the structural integrity in repeated contraction-expansion cycles, providing an ideal solution for long-term stable application in a physiological environment.

The utility model discloses nanofiltration equipment with a protective membrane function, the nanofiltration equipment with the protective membrane function comprises a water inlet pipe, an ORP meter (1), a nanofiltration module and a reducing agent adding part (3), the water inlet pipe is communicated with the nanofiltration module and is used for inputting water to be treated to the nanofiltration module, the ORP meter (1) is arranged on the water inlet pipe, and the reducing agent adding part (3) is arranged on the water inlet pipe. The ORP meter (1) is located at the front end of the nanofiltration module, the reducing agent adding component (3) is communicated with the water inlet pipe, and the ORP meter (1) is electrically connected with the reducing agent adding component (3); the ORP meter (1) is used for detecting the oxidation reduction potential in a water inlet pipe of the nanofiltration module and controlling the reducing agent adding component (3). The nanofiltration equipment with the protective membrane function can detect and find that residual chlorine is contained in a water body to be treated, Cl2 is changed into Cl <-> in an ionic state by adding a reducing agent, and the protective effect on a nanofiltration reverse osmosis membrane is achieved.

The application discloses a memristor and a preparation method and application thereof, which sequentially comprises a bottom electrode layer, a HOFs nanometer film functional layer and a metal top electrode from bottom to top; the thickness of the HOFs nanometer film functional layer is 80-120 nm; the film has a flat surface, good crystallinity and high orientation. As an active layer of the memristor, the film has good resistance state conversion performance, a low SET voltage of 0.86 V, excellent retention time of 1.64*10 4 s, good cycle performance of 60 times, and high thermal stability. The device benefits from the main hydrogen bond interaction and π-π conjugation in the structure, the film device preparation method is simple and perfect, the active functional layer PFC-73 can be recycled, the damaged device can be regenerated through a simple and direct dissolution recrystallization method, and the resistance state conversion performance is restored.

The invention relates to the technical field of antibiotic enzymes, and provides a peptide fusion antibiotic enzyme, the peptide fusion antibiotic enzyme comprises a catalytic domain LysS23, a cell-penetrating peptide and a connecting peptide, the catalytic domain LysS23 comprises an amino acid sequence represented by SEQ ID NO: 1, the cell-penetrating peptide comprises an amino acid sequence represented by SEQ ID NO: 2, and the connecting peptide comprises an amino acid sequence represented by SEQ ID NO: 3. Or an amino acid sequence which is obtained by substitution / deletion / addition of one or more amino acid residues on the amino acid sequence as shown in SEQ ID NO.1 and has more than 98% of identity is obtained by substitution / deletion / addition of one or more amino acid residues on the amino acid sequence as shown in SEQ ID NO.1. The catalytic domain LysS23 in the peptide fusion antibiotic enzyme has peptidoglycan lysis activity, the cell-penetrating peptide exerts a cell-penetrating function and has an outer membrane destruction effect, the cell-penetrating peptide acts on an extracellular membrane and helps the catalytic domain LysS23 to smoothly reach the peptidoglycan position of a cell wall so as to quickly exert the lysis effect, and the connecting peptide maintains the integrity of the structures and functions of the cell-penetrating peptide and the catalytic domain. Meanwhile, the antibacterial activity of the peptide fusion antibiotic enzyme is ensured. The peptide fusion antibiotic enzyme provided by the invention can directly act on common gram-negative bacteria without depending on the addition of a chemical outer membrane penetrant, exerts a good antibacterial effect, and has a very good application value.

The invention provides a membrane electrode based on a direct coating process and a preparation method and application thereof, and belongs to the technical field of fuel cells. The method comprises the following steps: directly coating cathode or anode catalyst slurry on one side of a proton exchange membrane with a temporary support substrate; then, the surface coated with the catalyst layer is continuously and directly coated with a proton exchange membrane functional layer and catalyst slurry, so that continuous construction of the proton exchange membrane and the catalyst layers on the two sides is achieved; finally, interface fusion and structure densification are promoted through annealing treatment, and the complete three-in-one membrane electrode is obtained. According to the method, a transfer printing film and a hot pressing transfer printing step needed in a traditional transfer printing process are completely abandoned, the manufacturing process is simplified, the material and energy consumption cost is reduced, and the production speed is remarkably increased. More importantly, by accurately controlling the coating sequence, the slurry formula and the drying-annealing process, swelling and deformation of the proton exchange membrane in the wet-dry cycle process are effectively inhibited, and it is ensured that the membrane electrode structure is smooth and the interface bonding is firm.

The invention belongs to the technical field of ion exchange membranes for capacitive desalination, and particularly relates to an ion exchange membrane as well as a preparation method and application thereof. The ion exchange membrane is prepared from the following raw materials in parts by mass: 5 to 15 parts of aminated alkyl modified MXene, 60 to 70 parts of styrene and 15 to 25 parts of 1, 3-butadiene. The composite ion exchange membrane has the beneficial effects that styrene and 1, 3-butadiene are used as organic monomers of the ion exchange membrane, functionalized MXene is used as an inorganic cross-linking reinforcement phase, and the composite ion exchange membrane is prepared through an ultraviolet-initiated polymerization-ionization modification process. Functionalized MXene and an organic monomer form a covalent cross-linked network, so that an ion channel between MXene layers is reserved, membrane swelling is inhibited through rigidity-flexibility balance of styrene / 1, 3-butadiene, and unification of rapid preparation and a high-structural-stability ion exchange membrane is realized.

The invention relates to the field of biological materials, in particular to a bionic biological valve. The bionic bioprosthetic valve is obtained by wrapping a cross-linked extracellular matrix material with a non-cross-linked biological basement membrane to form a sandwich structure. The basilar membrane and the cross-linked extracellular matrix material are synergistic, the excellent regeneration and repair performance and the characteristic of being rich in bioactive factors of the basilar membrane are fully exerted, growth and adhesion of endothelial cells on the surface of the material are effectively promoted, and a tissue structure imitating a natural valve leaflet is constructed. The biomimetic bioprosthetic valve shows excellent anti-calcification performance and excellent dynamic mechanical stability and compliance, significantly inhibits abnormal adhesion of platelets and blood cells, greatly improves the recovery and reconstruction efficiency of valve functions, can maintain structural integrity in repeated contraction-expansion cycles, and has good application prospects. And an ideal solution is provided for long-term stable application of the compound in a physiological environment.

The invention discloses a polypeptide for male contraception as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The polypeptide comprises a cell transmembrane functional region and an interference functional region derived from the carboxyl terminal of an angiotensin II type 2 receptor, and the amino acid sequence of the polypeptide is as shown in SEQ ID NO.1. An in-vitro experiment proves that the polypeptide can obviously inhibit an AT2R downstream beta-arrest signal channel, and the expression of a receptor is not influenced. In-vivo animal experiments further prove that the polypeptide can effectively reduce the sperm quantity and vitality of male mice, so that reliable contraception is realized, and the fertility can be completely recovered after drug withdrawal. The polypeptide is unique in action mechanism, reversible in effect and high in safety, and a core technical scheme is provided for developing non-hormone male contraceptive drugs. And the preparation process is mature, is easy for large-scale amplification, and has good clinical transformation potential and market application prospect.

The invention belongs to the field of impermeable membranes, particularly relates to a three-dimensional composite one-way drainage impermeable membrane, and aims to overcome the defects that an existing impermeable membrane is single in function and cannot meet different use requirements. The three-dimensional water and air guide net is arranged at the bottom of the lower-layer geotechnical cloth; the upper-layer geotechnical cloth is arranged above the lower-layer geotechnical cloth; the geomembrane is arranged between the lower-layer geotextile and the upper-layer geotextile; a water guide duct is formed between the three-dimensional water guide and air guide net and the geomembrane; the one-way drain valve is arranged on the geomembrane and matched with the water guide duct; according to the invention, exhaust and drainage pressure unitization and normalization are taken as a core technical target, functions of seepage prevention, water collection, water guide, exhaust, drainage, reinforcement and the like are integrated, engineering problems such as high groundwater, gas jacking, reservoir flatulence, channel freeze thawing and frost heaving and the like are effectively solved, and meanwhile, the construction cost is reduced. The method has the characteristics of convenient transportation, simple construction, cost saving and the like.

An object of the present disclosure is to make it possible to excellently join a functional part to a substrate even when warpage of the functional part has occurred. An electronic device (10) includes a functional part (102), a substrate (130) that supports the functional part (102), a first metallic film (110) as an electrode deposited on a side of the functional part (102) facing the substrate (130), and a second metallic film (140) which is formed on the substrate (130) and to which the first metallic film (110) is joined. The functional part (102) includes a first surface (102a) that faces the substrate (130) via the first metallic film (110) and a second surface (102b) that extends from the first surface (102a) in a direction different from an extending direction of the first surface (102a). The first metallic film (110) is deposited from the first surface (102a) of the functional part (102) to a part of the second surface (102b).

The present invention discloses a sterilization iodophor compound composition and a processing technology thereof, and relates to the technical field of sterilization, xanthan gum is added into deionized water, stirring is performed until complete dissolution, cooling is performed, glycerol and an amino-polyethylene glycol-polyacrylic acid block copolymer are added, stirring is performed until complete dissolution, povidone iodine is added, stirring is performed until complete dissolution, and the sterilization iodophor compound composition is obtained. And adding the microcapsule solution, adding deionized water, stirring, keeping out of light, standing and filtering to obtain the iodophor compound composition for sterilization. The povidone iodine can oxidize and destroy bacterial protein and nucleic acid by providing free iodine, so that a broad-spectrum bactericidal effect is achieved; thymol and eugenol destroy bacterial cell membranes and cell walls and inhibit key enzyme activity so as to realize a bactericidal effect; tannic acid is combined with protein to form a compound on a cell membrane to destroy the membrane function of the cell membrane; through the synergistic effect of the three components, the sterilization effect of the iodophor compound composition for sterilization is improved.

The application discloses a spider neurotoxin tail peptide with cell membrane penetration function and application thereof, wherein the spider neurotoxin tail peptide with cell membrane penetration function has an amino acid sequence as shown in SEQ ID NO: 2. The application further provides application of the cell membrane penetration peptide in preparation of a cell membrane penetrable carrier for delivering a bioactive molecule. The application further provides a fusion protein composed of the spider neurotoxin tail peptide and a spider neurotoxin omega-Pp1b and application of the fusion protein in preparation of an insecticide. The application further provides an amphiphilic peptide and application of the amphiphilic peptide in preparation of a cell membrane penetrable carrier for delivering a bioactive molecule. The application further provides a nanocomposite and application of the nanocomposite in preparation of a preparation for silencing of an insect cell gene. The application further provides a pesticide composition. The application is a novel cell membrane penetration peptide derived from an insect system itself, and has important significance for promoting development of peptide biological insecticides and RNA pesticides.

The invention discloses a composite membrane as well as a preparation method and application thereof, and relates to the technical field of polymer functional membrane materials. The composite membrane comprises a base membrane, a functional layer and a photo-thermal layer, the functional layer is arranged between the base membrane and the photo-thermal layer, the functional layer is formed by a cationic polyelectrolyte layer and an anionic polyelectrolyte layer which are alternately deposited on the surface of the base membrane, and the cationic polyelectrolyte layer comprises an amphiphilic chitosan derivative layer. The composite film provided by the invention can reduce the apparent evaporation enthalpy in the interface evaporation process and improve the evaporation efficiency.

The present invention relates to a multi-compartment microfluidic device comprising a first cellular compartment (10) within which a target mucosa of a mucosal organ is reproduced and a second cellular compartment (40) comprising neurons (400), the second cellular compartment (40) being connected to the first cellular compartment (10) by a means forming a biological interface (50) to allow communication via neuronal connection (401) between the first cellular compartment (10) and the second cellular compartment (40). Figure 1

The invention discloses an immobilized enzyme membrane reaction system and application thereof in preparation of flaxseed oligosaccharides, and an immobilized enzyme membrane assembly comprises a polyethersulfone ultrafiltration membrane and polygalacturonase which are connected through a covalent bond; an integrated system with high catalytic efficiency, excellent operation stability and good membrane function retentivity is constructed through collaborative design and fine regulation and control of a carrier interface, immobilized chemistry and reaction-separation coupling conditions, so that the existing limitation is broken through, and the method is suitable for industrial production. And a feasible technical scheme is provided for green, efficient and continuous production of flaxseed oligosaccharide and other products.