138 results about "Antifreeze protein" patented technology

A water ice comprising an antifreeze protein, a stabilizer and not less than 0.1 wt % of a protein based aerating agent obtainable by a process comprising aerating the ice confection with an aerating gas which contains at least 50% by volume of a water soluble gas such as carbon dioxide, nitrous oxide and mixtures thereof.

An unaerated ice confection in the form of thin, unsupported, discrete pieces which are stable during packaging, storage and distribution wherein the ice confection comprises an antifreeze protein and has the following mechanical properties;DELTA modulus / original modulus>=0.4, and / orDELTA strength / original strength>=0.4;providing that when DELTA modulus / original modulus<=6.0,DELTA modulus>=50 MPa, and / or whenDELTA strength / original strength<=2.0,DELTA strength>=0.2 MPa.

The invention belongs to the industrial field of quick-frozen foods, and in particular relates to a quick-frozen dumpling and a preparation method thereof. The novel quick-frozen dumpling comprises a dumpling wrapper and dumpling stuffing, wherein the dumpling wrapper is made from flour, coarse cereals flour, vegetable protein powder, anti-freezing protein, modified starch, an enzyme preparation, vegetable polysaccharide and water; and the dumpling stuffing is made from meat, vegetables, a seasoner, modified starch, vegetable proteins and water. The quick-frozen dumpling is even and reasonable in nutrition collocation, abundant in nutrition, and delicious in taste. The novel quick-frozen dumpling is fresh in taste and good in color and luster under the freezing status, can notbe frozen to be cracked, and can not degrade the dumpling wrapper, the electric energy can be saved by more than 20% during producing, and the multiple effects for improving the high quality, saving the energy, and reducing the consumption are realized.

The invention aims to provide a freezing preservative solution prepared by adopting natural components. The cell freezing preservative solution is prepared by the following raw materials in parts by weight: 10-18 percent of sodium chloride, 3-7 percent of ethanol, 2-6 percent of chitin, 5-8 percent of glycerol, 3-5 percent of cane sugar, 8-12 percent of antifreeze protein AFs and the balance of water. According to the cell freezing preservative solution, when various aquatic products and livestock meat foods are preserved at low temperature, breakage of cell walls caused by formation of ice crystals can be avoided, so that an aim of preserving the aquatic products and livestock meat foods is achieved. Moreover, the preservative solution is low in cost of raw materials and excellent in preservative effect.

The invention relates to a freezing liquid for human stem cells and a freezing storage method. Every 1000 ml of the freezing liquid comprises the following components: 20-50 mg of salidroside, 50-100 g of hydroxyethyl modified starch 450 Kd, 10-50 g of carboxylated poly-L-lysine, 50-100 g of sucrose, 20-150 g of trehalose, 5-20 g of antifreeze protein, 5-20 g of glycoprotein, 20-50 ml of DMSO, 60-100 ml of glycerin, and 200-300 ml of fetal calf serum, with the balance being a buffer solution. The freezing liquid for human stem cells is low in dimethyl sulfoxide content, has no side or toxic effect on frozen cells, can effectively keep the activity of stem cells, prevents oxidative injury, and is high in cell thawing rate.

The invention relates to a freezing diluent for a seminal fluid of livestock. Every 100 ml of the freezing diluent comprises components as follows: 2.71 g of trihydroxymethyl aminomethane, 1.4 g of citric acid, 1.0 g of monosaccharide, 5-20 ml of fresh yolk, 0-10 ml of a permeable protecting agent, 0.1 million IU of penicillin, 0.1 million IU of streptomycin, 0.05-5 g of polyvinyl alcohol and the balance of ultrapure water. The common high-molecular polymer polyvinyl alcohol serving as an ice crystal inhibitor replaces natural antifreeze protein and is applied to the cryopreservation research of the seminal fluid of livestock for the first time. The survival rate of unfrozen sperms is about 75%, the activity is 50% or higher, the acrosome completeness is 65% or higher, the plasma membrane completeness is about 50%, and the non-return rate after artificial insemination is 70% or higher.

The invention discloses a fresh micro-freezing liquid used for keeping food fresh. The fresh micro-freezing liquid used for keeping the food fresh comprises the following components, by weight, 4% of sodium chloride, 10% of ethyl alcohol, 0.8% of chitosan, 0.3% of antifreeze protein, and the balance water. The fresh micro-freezing liquid used for keeping the food fresh can achieve quick freezing of frozen food after the frozen food is immersed, and is high in freezing quality.

The invention relates to a preparation method of fish skin antifreeze protein, fish skin antifreeze protein prepared by adopting the method, and applications of the fish skin antifreeze protein. The method comprises the steps of beating fish skins into fish skin paste, mixing the fish skin paste with water and then milling into fish skin paste liquor; centrifuging the fish skin paste liquor to remove the upper-layer fat to obtain defatted fish skin paste liquor; performing ultrasonic treatment to the defatted fish skin paste liquor; mixing the ultrasonically-treated defatted fish skin paste liquor with compound protease for performing enzymatic hydrolysis; preserving heat, cooling and centrifuging, and collecting supernatant; micro-filtering the supernatant to obtain fish skin protein liquor; condensing and freeze-drying the fish skin protein liquor to obtain the fish skin anti-freeze protein. Aiming at the current development situation of the existing antifreeze protein products, the invention discloses a simple and efficient preparation method for fish skin antifreeze protein by taking fish skins as raw materials through a great amount of experiments; the prepared fish skin antifreeze protein has better specific anti-freeze function, easily realizes the industrial production, thus having tremendous application prospects and market values.

Antifreeze proteins, active fragments of these antifreeze proteins and mimetics thereof for use in inhibiting clathrate hydrate formation and reformation and designing and selecting inhibitors of clathrate hydrate formation and reformation are provided.

The invention discloses a preparation method of a frozen diluent of sheep semen and an application of the preparation method. The preparation method of the frozen diluent of the sheep semen comprisesthe following steps: firstly, accurately weighing tris(hydroxymethyl) aminoethane, dihydrate trisodium citrate, fructose, an inositol compound, ethanediol or glycerinum, and oxytocin, adding the materials into ultrapure water, dissolving, adding a fresh yolk centrifuged liquid supernatant, penicillin and streptomycin, mixing evenly, fixing the volume after adjusting the pH value to 6.8-7.2, thus obtaining the frozen diluent of the sheep semen. The inositol compound is used to replace an antifreeze protein, the defect of the application of the antifreeze protein is avoided, meanwhile, the oxytocin is added when the semen is conveyed, and the sperm vitality and the acrosomal integrity are improved, so that the fertility rate is increased.

Methods and compositions are provided for enhanced signal intensity and storage stability of standard nucleic acid amplification buffers, real-time PCR buffers or both. Buffers in accordance with the present invention include anti-freeze protein(s) (AFPs), optionally with a carrier protein, such as BSA.

The invention discloses a multifunctional liquid fertilizer capable of degrading pesticide residues and a preparation method thereof. The multifunctional liquid fertilizer is prepared from the following components in parts by weight: 5-10 parts of a nitrogen-containing component, 10-20 parts of a phosphorus-containing and potassium-containing component, 2-3 parts of a chelated medium and trace element component, 3-5 parts of amino acid, 8-10 parts of an organic matter, 10-15 parts of humic acid, 10-15 parts of a pesticide detoxifying enzyme, 0.5-2 parts of antifreezing protein, 0.5-1.5 parts of antibacterial peptides and 35-42 parts of water, wherein the pesticide detoxifying enzyme is prepared by mixing protease, cellulase, pectinase, catalase, amylase, glycerin, trimethylolethane, pentaerythritol, xylitol, sorbitol and water. The multifunctional liquid fertilizer can effectively degrade the pesticide residues and reduce the using amounts of pesticides, and can prevent plant diseasesand insect pests and maintain the balance of agroecological environment, so that the agricultural yield and the agricultural income are increased, the quality of agricultural products is improved andthe agricultural production environment is improved; the multifunctional liquid fertilizer has the advantages of comprehensive and balanced nutrition, high plant absorption and utilization rate, wideapplication range, simple preparation technology, economic benefit, high efficiency, environmental protection and the like.

The invention relates to the field of cell freezing liquids, and concretely relates to a cell cryopreservation liquid containing antifreeze proteins. AFP134 proteins are added to the cell cryopreservation liquid in order to effectively improve the cell recovery rate; and the cell cryopreservation liquid contains no DMSO, so the influences of the DMSO on the cytotoxicity are reduced.

The invention discloses an antifreeze polypeptide prepared from fish-derived collagens and a preparation method thereof. The preparation method is characterized by using fish-derived collagens as raw materials, adopting alkaline protease to carry out enzymolysis on the fish-derived collagens and carrying out separation and purification and freeze drying, thus obtaining the antifreeze polypeptide. The amino acid complete sequence of the antifreeze polypeptide is Gly-Ala-Pro-Gly-Ala-Gln-Gly-Pro-Pro-Gly-Leu-Gln-Gly-Met-Pro-Gly-Glu-Arg-Gly-Ala-Ala-Gly-Leu-Pro. By adopting the antifreeze polypeptide and the preparation method, the research ideas and methods of antifreeze proteins (polypeptides) existing at home and abroad are broken through, limitation of the quantity of the antifreeze proteins purified form natural organisms and considerations of international FDA (food and drug administration) for security of transgenic antifreeze proteins in food application are overcome, an efficient antifreeze polypeptide based on food sources is obtained, and a theoretical foundation is laid for developing the antifreeze polypeptide based on food sources and exploring extensive application of the antifreeze polypeptide to food and medicines.

The invention discloses a processing method of meat products. Belonging to the technical field of meat processing, the invention relates to an anti-freezing method for quick-frozen fresh processed meat products. The method improves the existing technologies by adding a composite additive in the rolling and rubbing process. The composite additive comprises: based on the total amount of rolling and rubbing powder, 0.15-0.2 weight per thousand of antifreeze protein, 0.3-0.5 weight per thousand of xanthan gum, 0.3-0.5 weight percent of white sugar and 0.5-1 weight percent of mycose. After thawing, the fresh processed meat products have little loss of moisture, high content of nutritional components, bright appearance, as well as unique and delicious flavor, thus being popular with consumers.

The invention belongs to a qualitative detection technology of plant derived components in the foods and the beverages, in particular to a primer / a probe group for detecting a carrot component in the foods and the beverages. In the invention, a group of specific primer and probe are designed aiming at antifreeze protein of the carrot and according to the conserved sequence of a gene thereof. The carrot component in the foods and the beverages can be rapidly, sensitively and specifically detected by using the primer and the probe and adopting a real-time fluorescence PCR technology. The primer and the probe can be provided with other reagents together in the form of a kit and are used for amplification reaction of nucleic acid. The method has simple operation and good repeatability.

The invention relates to the cell field, in particular to cell cryopreservation composition and an application thereof. Antifreeze protein is used in a cell cryopreservation liquid, so that the survival rate of cells is increased significantly. The antifreeze protein is added to the antifreeze composition, the survival rate can be significantly increased to 80% or above, the composition has greatsignificance in preserving rare cells such as stem cells, nerve cells and the like, meanwhile, the cell culture cost is also reduced after cell thawing, and the cell culture cycle is shortened.

Isolated polynucleotides encoding antifreeze polypeptides are provided, together with expression vectors and host cells comprising such isolated polynucleotides. Methods for the use of such polynucleotides and polypeptides are also provided.

The invention discloses frozen bread dough containing compound plant antifreeze proteins, a preparing method thereof and a method for preparing bread through an optimized process. The frozen bread dough is prepared from, 40-50 wt% of bread special flour, 9-12 wt% of sugar, 0.5-1.5 wt% of yeast, 0.4-0.8 wt% of sodium chloride, 1.5-3 wt% of milk powder, 5-11 wt% of shortening, 0.05-0.15 wt% of fenugreek gum, 0.05-0.30 wt% of monoglycerides tartartaric acid diacetate, 20-30 wt% of water, 0.25-1.5 wt% of carrot antifreeze protein powder, 0.5-3 wt% of Tangut rhodiola rosea leaf antifreeze protein powder and 0.5-3 wt% of polygonum viviparum leaf antifreeze protein powder. The bread produced after the bread antifreeze dough is unfrozen, leavened and baked has the advantages of being large in specific volume, crispy, soft and delicious, and extremely excellent in sensory quality.

The invention provides an amphiphilic anti-freeze molecule based on silsesquioxane, a preparation method thereof and an anti-icing surface material and belongs to the technical field of anti-icing surface materials. The invention solves the problem of an instable anti-icing effect of an anti-icing surface material in the prior art. The amphiphilic anti-freeze molecule is prepared by combining the silsesquioxane with an anti-freeze protein or an anti-freeze polypeptide. The invention also provides the preparation method of the amphiphilic anti-freeze molecule based on silsesquioxane. The method includes the steps of preparing the silsesquioxane having reactive groups and hydrophobic groups, and performing a coupling reaction to combine the silsesquioxane with an anti-freeze protein or anti-freeze polypeptide to prepare the amphiphilic anti-freeze molecule based on the silsesquioxane. The invention also provides the anti-icing surface material prepared from the amphiphilic anti-freeze molecule based on the silsesquioxane. The anti-icing surface material has the anti-icing characters of both a hydrophobic material and a hydrophilic material, enlarges a temperature window of the anti-icing surface material and is improved in anti-icing performance.

Anti-freeze protein which can be derived from Lichen, said protein having an apparent molecular weight of from 20 to 28 kDa and having an N-terminal amino acid sequence which shows at least 80% overlap with: A-P-A-V-V-M-G-D-A-E-S-F-G-A-I-A-H-G-G-L and modified versions and isoforms of this protein.

The invention relates to an aquatic product anti-staling agent. The anti-staling agent contains the following components in percentage by mass: 0.035-10% of antifreeze protein, 10-25% of trehalose, 1-3.5% of chitosan, 1-3.5% of phytic acid, 0.5-1% of sodium erythorbate and the balance of water. After fresh small cuttlefish, sleeve-fish, nemipterus virgatus and whiteleg shrimps fished ashore are soaked with the anti-staling agent disclosed by the invention, the fishes are packaged in vacuum and frozen at the temperature of being lower than or equal to 18 DEG C, and due to storage of 3-4 months, tissues of the unfrozen fishes are still tight and enriched in elasticity, eye balls of the fishes are full and glossy, the body surface color is bright, any darkening or fading phenomenon is avoided, fresh seaweed smell is maintained, and the moisture retaining effect is excellent.

The invention provides an antifreeze polypeptide and preparation method thereof. The method takes cow leather collagen as raw material and obtains the specific antifreeze polypeptide by screening and optimizing the enzymolysis condition of alkali protease, separating and purifying. The antifreeze polypeptide has the molecular weight of 2107Da and the amino acid full sequence of Gly-Glu-Arg-Gly-Phe-Pro-Gly-Glu-Arg-Gly-Ser-Pro-Gly-Ala-Gln-Gly-Leu-Gln-Gly-Pro-Arg. The invention breaks through the research thinking and method of antifreeze protein at home and abroad, overcomes the limitation of the number of purified antifreeze protein in natural organism and the safety worry of international FDA organization for transgene antifreeze protein in the food application, obtains the food-source high-efficiency antifreeze polypeptide, and lays the theoretical foundation for developing the antifreeze polypeptide based on the food source and exploring the wide application of the antifreeze polypeptide in the aspects of food and medical science.

The invention discloses frozen bread dough containing a composite antifreeze agent made from plant antifreeze proteins, a preparation method of the dough, and a method for preparing bread with an optimized process. The frozen bread dough comprises the following components in parts by weight: 40-55% by weight of bread specialized bread powder, 10-12.5% by weight of sugar, 0.5-1.5% by weight of yeast, 0.4-0.8% by weight of sodium chloride, 1.5-3% by weight of milk powder, 5-10.5% by weight of shortening, 0.05-0.15% by weight of bottle gourd Pakistan rubber, 0.05-0.30% by weight of diacetytartaric esters of monoglycerides, 20-30% by weight of water, 0.05-0.1% by weight of glutamine transaminase, 0.05-0.1% by weight of xylanase, and 0.5-3% by weight of antifreeze protein powder made of polygonum viviparum leaves. The produced frozen bread dough is subjected to thawing, curing and baking so as to form bread which has the characteristics of large specific volume, a soft and tasty mouth feeland excellent organoleptic quality.

The invention relates to a method for extracting antifreeze proteins, in particular to a method for extracting apoplast antifreeze proteins from winter rape roots. According to the method, apoplast antifreeze proteins are successfully extracted from cold-induced winter rape roots. The method for extracting apoplast antifreeze proteins from winter rape roots is characterized by comprising the following steps: (1) sampling; (2) extracting a buffer solution for preparing; (3) slitting; (4) immersing; (5) vacuumizing; and (6) centrifuging. According to the method, apoplast antifreeze proteins are successfully and rapidly extracted from cold-induced winter rape roots, so that technical support is provided for further research of the cold resistance mechanism of winter rape.

The invention relates to probiotic goat milk tablets and a preparation method thereof. Medicinal and edible Chinese jujube polysaccharide is added to a culture medium to be used as a proliferation factor, a freezing-resistant factor and a freeze-drying protecting agent of probiotics, so that proliferation and freezing resistance of the probiotics are promoted; then at low temperature, cold treatment is performed, so that antifreeze protein produced through induction further improves the freezing resistance of the probiotics; and then through the composite freeze-drying protecting agent, an icecrystal point is reduced, and protecting effects on the probiotics are improved, so that high-activity probiotic powder is obtained. The probiotics added to the probiotic goat milk tablets are high in viable count, so that the number of the probiotics in intestinal tracts of consumers can be increased, and microbial population can be regulated to be balanced; and dietary fiber glucan and xylooligosaccharide which are added can promote proliferation of the probiotics in the intestinal tracts, absorption of minerals, promote squirm of the intestinal tracts and prevent constipation; and the added functional sweetening agent has low calorie, cannot cause decayed teeth or obesity, is free from influence on blood sugar, and has the functions of loosening bowel to relieve constipation, promotingcalcium absorption and the like.