37 results about "Antifreeze protein" patented technology

The invention discloses a rapid food preservation control method based on a flash freezing technology, and relates to the technical field of food preservation, and the method comprises the following steps: obtaining first data of food, and spraying a nano coating containing antifreeze proteins with different concentrations on the surface of the food; inputting the first data into a pre-trained neural network model to obtain second data; according to the second data, a refrigerant injection device and a pulse electric field excitation device are synchronously started, food dynamic data are obtained, feature extraction and trend prediction are conducted on the food dynamic data, and the food dynamic data comprise the real-time core temperature and the ice crystal formation dynamic state; according to the prediction result, the refrigerant flow rate and the electric field frequency are dynamically adjusted, and the release rate of antifreeze protein in the nano-coating is synchronously adjusted; according to the method, the refrigerant flow rate, the electric field frequency and the antifreeze protein release rate are accurately and synchronously adjusted, so that the problems of non-uniform freezing, overlarge ice crystals and food quality deterioration in a traditional freezing method are effectively solved, and the food freezing fineness and the fresh-keeping effect are improved.

The invention discloses a biochemical multi-item liquid quality control product containing 67 analytes and a preparation method of the biochemical multi-item liquid quality control product, and belongs to the technical field of biochemical quality control products, non-analytes of the quality control product comprise a cryoprotectant compound with the addition amount of 0.1-1%, the cryoprotectant compound comprises antifreeze protein and a protein stabilizer, and the antifreeze protein and the protein stabilizer are added into the quality control product. The mass ratio of the antifreeze protein to the protein stabilizer is (0.1: 1)-(0.5: 1); the biochemical multi-liquid quality control product is frozen liquid. According to the invention, antifreeze protein and elongation factor 1-alpha homologous protein cooperate through physical-chemical dual pathways, complete-cycle protection is provided for protein and enzyme in a quality control product aiming at'mechanical factor 'and'oxidation factor' of freezing damage, and inter-batch consistency and storage stability can be improved; and errors caused by redissolution can be avoided, the detection convenience and accuracy of inspectors are greatly improved, and the labor and time cost of a laboratory is reduced.

The invention discloses an altapialus altai antifreeze protein and application thereof, and relates to the technical field of freezing resistance. The amino acid sequence of the antifreeze protein is shown as SEQ ID NO: 1. The amino acid sequence of the antifreeze protein of the altapialus altai takes 76 amino acids as a unit, circulates for 7 times, mainly takes MQIFVKTLTGKTITLEVE as a main part, and has no homology with antifreeze proteins of previously reported insects.

The invention discloses a preparation method for relieving cracks and white edges of quick-frozen dumpling wrappers based on vital wheat gluten antifreeze protein, and relates to the technical field of wheat deep processing, and the preparation method comprises the following steps: preparing GAFP; determining free amino acids; observing an ice crystal structure; carrying out hydrophilic analysis; making dumpling wrappers, and performing freeze thawing treatment; analyzing the influence of GAFP on the moisture content of the dumpling wrappers; the antifreeze protein and the antifreeze polypeptide are prepared by taking wheat processing byproduct vital gluten as a raw material and adopting an enzyme method, so that the antifreeze protein and the antifreeze polypeptide have a protection effect on quick-frozen dumpling wrappers in a freezing and thawing period, a theoretical basis is provided for developing wheat source functional protein products, high-valued application of wheat processing byproducts is promoted, a wheat industrial chain is prolonged, and the antifreeze protein has important economic and social significance and has a good application prospect. The anti-freezing protein and the anti-freezing polypeptide GAFP are prepared from wheat processing by-product vital wheat gluten as a raw material by adopting an enzyme method, and the anti-freezing protein and the anti-freezing polypeptide GAFP have a protection effect on quick-frozen dumpling wrappers in a freezing and thawing period, so that the cracking and white edge prevention effects of the quick-frozen dumplings are improved, and the mouth feel of consumers is achieved.

The invention belongs to the technical field of hydrogel materials, and particularly relates to self-adjusting light-curing ice-cold hydrogel as well as a preparation method and application thereof.The self-adjusting light-curing ice-cold hydrogel is prepared from the following raw materials in parts by weight: 10-17 parts of a cool-feeling matrix, 18-33 parts of acrylamide 13C3, 27-42 parts of glycerol, 5 parts of TPGDA, 0.05-0.15 part of a cross-linking agent and 0.1-0.3 part of a photoinitiator, by constructing a synergistic system of antifreeze protein and a konjac glucomannan skeleton, the purpose of gelation and cool sense self-adjustment of an isotope labeling monomer is achieved, and the isotopic labeling monomer can be used for preparing drug-loaded gel dressings, cooling gel pads, cooling gel bags, cooling gel injection products and in-situ injection gel products. The photocuring cross-linking technology is beneficial to rapid forming of the gel dressing, and the stability of the effective components is improved.

The invention belongs to the technical field of embryo in-vitro culture, and discloses a method for in-vitro production of high-quality embryos by using frozen boar semen. In the in-vitro production process of the pig embryo, the fertilization rate and the quality of the in-vitro cultured embryo are improved by using the frozen semen treated by the antifreeze protein NT5C1B and the mature oocyte through in-vitro fertilization. Wherein the selected protein can reduce the generation of ice crystallization in the freezing process of the semen, improve the quality and fertilization ability of the frozen semen, and provide a basis for high-quality development of embryos after in-vitro fertilization. The application provided by the invention is simple and convenient to operate and remarkable in effect, and does not generate any harmful influence on animals. The invention provides a powerful theoretical basis for in-vitro production of high-quality embryos.

The invention relates to a method for quality-guaranteeing fermentation of fish meat through combination of electrolyzed water and antifreeze protein, and belongs to the field of fermented food. The fermented fish is soaked in a fresh-keeping solution composed of antifreeze protein and electrolyzed water, and the electrolyzed water is prepared by electrolyzing a solution of sodium chloride and sodium bicarbonate. The electrolyzed water prepared from the sodium chloride and sodium bicarbonate solution is used for treating the fermented fish meat, so that the appearance and color degradation of the fermented fish meat under the action of the electrolyzed water is avoided, and the accumulation of volatile basic nitrogen in the fermented fish meat is inhibited; the protein structure is protected through antifreeze protein, and the tenderness and texture characteristics of the frozen and thawed fermented fish meat are maintained; through the synergistic anti-oxidation effect of the electrolyzed water and the antifreeze protein, oxidation deterioration of color, smell, taste and appearance in the frozen transportation process of the fermented fish meat is remarkably relieved, and the fresh-keeping and quality-keeping requirements of the easily-decayed fermented fish meat in the frozen long-distance transportation process are met.

To provide frozen food which can be thawed and tastily taken, and a method for producing the same.SOLUTION: A method for producing frozen food includes: a rice cooking step (S101) of cooking rice using water added with antifreeze protein; a sushi rice step (S106) of mixing the rice obtained by the rice cooking step with blended vinegar; a molding step (S107) of molding the rice obtained by the sushi rice step into a boiled rice ball; a sushi ingredient step (S108) of topping the boiled rice ball obtained by the molding step with sushi ingredients; and a freezing step (S109) of freezing the sushi obtained in the sushi ingredient step while applying vibration to the sushi.SELECTED DRAWING: Figure 1

The invention discloses a method for raising sheep in alpine regions and a special feed thereof. The invention relates to a diatom-source antifreeze protein peptide-III-microencapsulated calcium palmitate-containing compound preparation, which specifically comprises the following components in parts by mass: 100 to 130 parts of microencapsulated calcium palmitate, 6 to 10 parts of diatom-source antifreeze protein peptide AFP-III, 0.01 to 0.1 part of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) edited lactobacillus plantarum LP-7, 1 to 2 parts of selenium-loaded mesoporous hydroxyapatite, 1 to 1.5 parts of dimercaptosuccinic acid, 0.1 to 0.3 part of resveratrol-beta cyclodextrin inclusion compound, and 500 to 600 parts of highland barley straw. The invention relates to the technical field of feeds, in particular to a cold-resistant feed additive which comprises, by mass, 1-1.5 parts of complex microbial inoculants, 50-100 parts of cold-resistant composite extracts and 20-40 parts of rare earth modified bentonite. According to the breeding method and the special feed for the sheep in the alpine region, highland barley straw is fermented by adopting an aspergillus niger and xylanase complex microbial inoculant in stages, so that the degradation rate of lignin is greater than or equal to 76%, the lignocellulose structure of the straw is greatly damaged, and fermentable carbohydrates are exposed; the palatability and the feed intake of the coarse feed are improved.

To provide a method for producing frozen food suitable for freezing rice cooked with an antifreeze protein. [Solution] This is a method for producing frozen foods, which includes a rice cooking process (S104) in which rice is cooked using water to which antifreeze protein has been added, a molding process (S106) in which the rice obtained in the rice cooking process is shaped into sari balls, a freezing process (S107) in which the sari balls obtained in the molding process are frozen, and a glazing process (S108) in which a mist of liquid is sprayed onto the surface of the sari balls frozen in the freezing process to freeze them.

The invention discloses an activity maintaining process for deep low-temperature long-term cryopreservation of autologous bones, and belongs to the technical field of autologous bone preservation, and the autologous bones comprise the following raw materials in parts by weight: 1-2 parts of L-proline, 5-8 parts of a PVP / WS2 nano composite material, 0.1-0.5 part of a matrix metalloproteinase (MMPs) inhibitor, 1-2 parts of antifreeze protein (AFPs), 1-2 parts of an impermeable protective agent, 1-2 parts of trilauryl methyl ammonium chloride and 2-3 parts of an oxygen carrier. According to the autologous bone cryopreservation culture medium, the use of a traditional cryoprotectant DMSO can be reduced, the toxicity of the traditional cryoprotectant DMSO to cells and tissues is reduced, the damage of ice crystals to the cells in the cryogenic long-term cryopreservation process is effectively relieved, the integrity of collagenous fibers in bone tissues is maintained, and the reduction of the activity of growth factors is inhibited.

The invention discloses a microbial freeze-drying embedding method based on a pH-triggered phase change mechanism. The method comprises the following steps: step 1, preparing a zein solution: dissolving zein into a sodium hydroxide solution; step 2, preparing a bacterial suspension: taking fresh excrement of a healthy donor as a raw material, diluting with normal saline, uniformly stirring and mixing, and filtering with a multi-stage filter screen to obtain the bacterial suspension; 3, preparing an intestinal flora freeze-drying protective agent: dissolving lactose, maltotriose, mannitol, trehalose and antifreeze protein in normal saline according to a ratio, and sterilizing for later use; step 4, constructing a composite system: centrifugally washing the bacterial suspension, adding a freeze-drying protective agent, uniformly mixing in a vortex manner, adding a zein solution in proportion, and sufficiently and uniformly mixing; and step 5, freeze-drying treatment: performing vacuum freeze-drying on the composite system to form a compact hydrophobic protective layer, thereby obtaining the freeze-dried intestinal flora powder. According to the invention, the pH response characteristic of the protein is coupled with a freeze-drying process, so that efficient embedding protection of intestinal flora is realized.

The present invention discloses an active maintenance process for cryopreserving autologous bone at deep low temperature, belonging to the technical field of autologous bone preservation. The raw materials by weight include: 1-2 parts of L-proline, 5-8 parts of PVP / WS2 nanocomposite, 0.1-0.5 part of matrix metalloproteinase (MMPs) inhibitor, 1-2 parts of antifreeze protein (AFPs), 1-2 parts of non-permeating protective agent, 1-2 parts of trimethylammonium lauryl chloride, and 2-3 parts of oxygen carrier. This autologous bone cryopreservation medium can reduce the use of the traditional cryoprotectant DMSO, reduce its toxicity to cells and tissues, effectively slow down the damage of ice crystals to cells during long-term deep low temperature cryopreservation, maintain the integrity of collagen fibers in bone tissue, and inhibit the decrease in the activity of growth factors.

The high-boiling-point low-conductivity ultralow-temperature secondary refrigerant comprises the following components in percentage by mass: 5%-10% of antifreeze protein, 8%-15% of weak conductive salt, 1%-3% of a low-temperature protective agent, 0.15%-2.1% of a pH regulator, 15%-25% of a high-boiling-point aid and the balance of water, the weak conductive salt is sodium acetate and sodium propionate; the low-temperature protective agent is a trehalose or protease inhibitor; the pH regulator is composed of citric acid and sodium hydroxide; the high-boiling-point auxiliary agent is ethylene glycol or propylene glycol. The finally prepared secondary refrigerant is not prone to layering and solidification at the ultralow temperature, higher in stability, capable of avoiding system pressure sudden rise caused by boiling of the secondary refrigerant at the high temperature, low in conductivity, capable of using electricity safely, capable of avoiding the risk of electric leakage, low in corrosion rate and capable of avoiding corrosion loss of a traditional acid / alkaline secondary refrigerant to equipment and prolonging the service life of a system.

The invention discloses a design method of a methane hydrate small-molecule inhibitor based on an antifreeze protein inhibition mechanism, and the design process of an adsorption type target spot is as follows: HIS33, PHE59 and TYR71 are taken as templates, small molecules containing pyridine rings, benzene rings and phenolic hydroxyl groups as key characteristic structures are designed, hydrophobic groups and hydrogen bond donors / receptors are also contained, and the adsorption type target spot is prepared. Meanwhile, the cage spacing of methane hydrate is 0.742-0.990 nm, and spatial distribution of adsorption residues and the number of hydrogen bonds are used as geometric constraints of molecular design; the design process of the disturbance type target spot comprises the steps that molecules of a rigid multi-ring structure are designed on an ice bonding surface through a mechanism of disturbance inhibition of methane hydrate growth, and ordered arrangement of water molecules is disturbed through steric hindrance. The new insight is obtained through the mechanism difference between IBS and non-IBS, the mechanism of TmAFP for inhibiting methane hydrate growth can be further known, and help is provided for designing an anti-freezing material and a methane hydrate growth inhibitor with biological inspiration.

The invention relates to the field of agriculture, and discloses an application of a novel disease-resistant and yield-promoting biological agent, a preparation method comprises the following steps: screening a microbial strain and performing gene editing, so that the microbial strain overexpresses heat shock protein and antifreeze protein genes; carrying out microcapsule embedding on the strain subjected to gene editing, and adding a low-temperature protective agent; carrying out vacuum freeze drying on the embedded strain; nano-particles are prepared by using a nanotechnology, and a plant growth regulator is loaded; and mixing the dried microcapsule particles and the nanoparticles loaded with the plant growth regulator, adding a buffering agent and an antioxidant, and packaging by adopting an intelligent packaging material. A polylactic acid-glycolic acid copolymer is utilized to form a physical barrier to wrap a strain, glycerin and trehalose are matched to reduce the freezing point and stabilize the cell structure, ice crystal damage and environmental impact are jointly resisted in a low-temperature environment, the problem that the strain is prone to inactivation in low-temperature storage and transportation of a biological preparation is solved, and the survival rate of the strain and the stability of the preparation are remarkably improved.

The invention belongs to the technical field of hydrogel materials, and specifically relates to a self-regulating photo-curable cooling hydrogel and a preparation method and an application thereof. The self-regulating photo-curable cooling hydrogel comprises the following raw materials in parts by weight: 10-17 parts of cooling matrix, 18-33 parts of acrylamide-13C3, 27-42 parts of glycerol, 5 parts of TPGDA (tripropylene glycol diacrylate), 0.05-0.15 parts of crosslinking agent, and 0.1-0.3 parts of photoinitiator. By constructing a synergistic system of antifreeze protein and konjac glucomannan skeleton, the gelation of an isotope-labeled monomer and the purpose of self-regulating cooling are achieved. It can be used for preparing drug-loaded gel dressings, cooling gel dressings, and in-situ injectable gel products. The photo-curable crosslinking technology facilitates the rapid molding of gel dressings and improves the stability of active ingredients.

The invention relates to the field of aquatic products, in particular to a deep hypothermia preservation method for black carp eggs. By microinjection of saccharides such as trehalose or polysaccharide or antifreeze protein (AFP) extracted from deep-sea fishes, formation of ice crystals in the freezing process is reduced, and the survival rate of ova and embryos is increased. The ovum and the embryo are encapsulated by using sodium alginate hydrogel to form a cell spherical structure, so that the problems of mechanical damage and dehydration in the freezing process are reduced. In addition, DMSO or other low-temperature protective agents with different concentrations are added step by step, damage of osmotic pressure to the ovum and the embryo is reduced, a transparent glassy state is formed in liquid nitrogen, formation of ice crystals is avoided, and the preservation effect is improved. The ova preserved by the deep hypothermia preservation method of the fish ova or embryo is complete in form, and the proportion of the ova with water absorption capacity reaches 82.3%.

The invention discloses a beef cattle embryo ultralow temperature biological preservation method based on gradient cooling control, and belongs to the field of beef cattle artificial breeding. The mixed solution comprises an equilibrium solution and a cryoprotectant, the equilibrium solution comprises a base solution and fetal calf serum, and the base solution comprises trehalose and polyvinylpyrrolidone; the cryoprotectant comprises the following components: dimethyl sulfoxide, glycerol and antifreeze protein, and the cryoprotectant accounts for 0.5-2% of the total volume of the mixed solution. And balancing the bovine embryos in the mixed solution for 10-15 minutes, and then carrying out ultralow-temperature preservation by adopting a gradient cooling mode. According to the method, the permeation protective agent and the non-permeation protective agent are combined for use, the formula components and the dosage of the permeation protective agent are optimized, meanwhile, a gradient cooling mode is combined, in the stage of starting to form ice crystals, a low-speed rotation mode is combined with gradient cooling, so that the formation of the ice crystals is remarkably reduced, and the embryo preservation effect is improved.

The invention discloses preparation and application methods of a nutrient solution for preventing and treating low-temperature freeze injury of tomatoes, and belongs to the technical field of agricultural fertilizers. The nutrient solution for preventing and treating the low-temperature freezing injury of the tomatoes is prepared from the following raw materials in parts by mass: 5 to 10 parts of triacontanol, 3 to 10 parts of gamma-aminobutyric acid, 10 to 25 parts of proline, 3 to 7 parts of linolenic acid, 30 to 40 parts of potassium nitrate, 5 to 10 parts of potassium silicate, 15 to 20 parts of calcium superphosphate, 20 to 30 parts of calcium chloride, 1 to 5 parts of zinc sulfate, 1 to 5 parts of copper sulfate, 5 to 10 parts of manganese sulfate and 150 to 250 parts of water. The nutrient solution for preventing and treating the low-temperature freezing injury of the tomatoes is beneficial to solving the problem that the yield and quality of the tomatoes are easily influenced by low-temperature stress, and can promote plants to synthesize antifreeze proteins and osmotic regulators, reduce the membrane lipid phase transition temperature and greatly improve the stress resistance of the plants.

The invention discloses a method for improving cold resistance of michelia figo, and belongs to the technical field of michelia figo cold resistance. A cold-resistant agent is prepared by adopting a gel matrix material to load inositol, proline and antifreeze protein, the cold-resistant agent is sprayed to michelia figo plants before late spring cold comes, the cold-resistant agent is gelatinized and attached to the body surfaces of the plants after being sprayed, and the michelia figo cold resistance is improved. Effective components such as inositol, proline and antifreeze protein are continuously released, the effective components improve the cold resistance of the plants by improving the enzyme activity in michelia figo plants and maintaining the stability of cell membranes, then the normal growth condition of the plants in the late spring cold period is maintained, the temperature rises after the late spring cold, the cold-resistant agent is solated and runs off from the surfaces of the plants, and the cold resistance of the michelia figo plants is improved. Normal growth of later plants cannot be influenced; the mixed liquid fertilizer is sprayed after late spring coldness to restore the good tree vigor of michelia figo plants and promote the good growth of michelia figo, and the method has important significance in promoting the development of the michelia figo industry.

The present invention relates to the field of plant growth regulators and provides the use of Candida to enhance the effects of antifreeze proteins. The antifreeze proteins have the effect of enhancing the cold tolerance of crops. The Candida is Candida XHZG06-95A3, which is taxonomically categorized as Candida palmioleophila and has the accession number CGMCC No. 29215. The amino acid sequence of the antifreeze protein is set forth in SEQ ID NO: 1. In the present invention, the use of Candida XHZG06-95A3 and / or its fermentation supernatant can enhance the effects of the antifreeze proteins, thereby further improving the cold tolerance of crops.

The invention discloses a low-temperature quick-freezing partial freezing solution and a fresh-keeping method, and relates to the technical field of quick-freezing and fresh-keeping of food, and the low-temperature quick-freezing partial freezing solution comprises a component A and a component B, the component A is prepared from the following raw materials in parts by mass: 40 to 56 parts of propylene glycol, 35 to 44 parts of water, 0 to 24 parts of ethanol, 0.2 to 3 parts of glycerol, 0.02 to 0.05 part of Nisin nisin, 5 to 8 parts of plant polysaccharide, 0.5 to 1 part of hyperbranched polylysine, 0.6 to 0.9 part of food-grade chitosan quaternary ammonium salt, 0.8 to 1.2 parts of adenosine, 0.01 to 0.05 part of vitamin C, 0.3 to 0.6 part of sorbitol, 3 to 5 parts of inorganic salt and 1 to 2 parts of cane molasses; and the component B comprises the following components in percentage by mass: 0.3-0.5% of antifreeze protein and the balance of water. The low-temperature quick-freezing partial freezing liquid and the freshness locking method are obvious in freezing and fresh-keeping effect, and safe and environment-friendly to use.

The invention discloses a preparation method of a nematodidae fish type-III antifreeze protein based on an escherichia coli strain, and belongs to the technical field of biology. The nucleotide sequence for coding the type-III antifreeze protein of the nematodes fish is shown as SEQ ID NO. 2. The preparation method of the three-type antifreeze protein of the nematodes fish comprises the following steps: (1) constructing the nucleotide sequence into an expression vector pET-28a to obtain a recombinant plasmid; and (2) transforming the recombinant plasmid into an escherichia coli BL21 (DE3) strain, and carrying out liquid culture, inducible expression, bacterial liquid collection, extraction and purification to obtain the type-III antifreeze protein of the nematodes fish. According to the antifreeze protein expressed and purified on the basis of the escherichia coli BL21 (DE3) strain, according to gene design after multi-sequence alignment, a prokaryotic expression vector escherichia coli is used, and large-scale expression and purification of the three-type antifreeze protein of the nematodes fish are achieved.

The present invention relates to a coolant comprising a) at least one antifreeze protein, b) at least one oligosaccharide, preferably at least one disaccharide, and c) at least one aqueous saline solution. The invention also relates to the use of at least one coolant comprising a) at least one PAG antifreeze protein for cooling a battery, preferably a lithium-ion battery, and / or a motor, preferably an electric motor, for a motor vehicle.

The invention provides an ultralow-temperature cryopreservation method for ram semen, and relates to the technical field of animal breeding biology. The ultralow-temperature cryopreservation method for the ram semen comprises the following steps: collecting fresh ram semen, centrifuging to remove seminal plasma, and adjusting the sperm concentration to 1 * 10 < 8 >-2 * 10 < 8 > / mL by using a basic diluent containing yolk and calf serum; mixing the pretreated semen with a composite cryoprotectant according to a volume ratio of 1: 1; and sequentially balancing at 37 DEG C for 15 minutes, balancing at 4 DEG C for 30 minutes, freezing at-80 DEG C for 20 minutes, subpackaging into thin tubes, and putting into liquid nitrogen (-196 DEG C) for storage. By optimizing the formula of the composite cryoprotectant (low-toxicity DMSO + trehalose + tea polyphenol + selenomethionine + antifreeze protein) and a gradient cooling process, the problems of high toxicity and insufficient oxidation resistance of the protectant in the traditional method are solved. The method can significantly improve the vitality and acrosome integrity of the unfrozen ram semen, is suitable for long-term preservation of different varieties of ram semen, and provides a new technology for livestock and poultry germplasm resource protection.

The invention belongs to the field of antifreeze proteins, and particularly relates to a transmembrane antifreeze fusion polypeptide, application, a cell low-temperature cryoprotectant and a cryopreservation method. The transmembrane antifreeze fusion polypeptide contains an antifreeze functional fragment and a transmembrane functional fragment. According to the application, the cell-penetrating polypeptide and the target antifreeze polypeptide are fused to construct the antifreeze polypeptide with cell membrane penetrating capacity. The synthesized polypeptide has good ice crystal recrystallization inhibition capability and ice crystal morphology modification capability. The antifreeze protein is used as a cryoprotectant, especially is added into a cell cryoprotectant, and the cell survival rate can be improved by adopting the strategy of synergistic effect of the cell internal and external protectants.

The present invention discloses a method for predicting antifreeze protein typing based on transfer learning, belonging to the field of bioinformatics. The method of the present invention comprises: collecting domain-annotated antifreeze protein and non-antifreeze protein sequences as positive and negative data sets; adopting oversampling and undersampling methods to balance the data; selecting a ProteinBert model to train the antifreeze protein sequence data set, so that a correspondence is formed between the antifreeze protein sequence and the domain label, and constructing a BERT-DomainAFP model; inputting the protein sequence data to be identified into the BERT-DomainAFP model for prediction to obtain a corresponding domain prediction value; setting a target data label, and if the domain prediction value output by the BERT-DomainAFP model is the same as the target data label, it is determined that an antifreeze protein sequence domain exists in the protein sequence to be identified. The model of the present invention has a strong feature extraction capability and can automatically extract complex feature information from protein sequences, and has high accuracy, good specificity, high sensitivity, and high prediction precision in predicting antifreeze proteins.

To provide a method for producing frozen food suitable for freezing cooked rice mixed with antifreeze protein. [Solution] This is a method for producing frozen foods, which includes a rice cooking process (S104) in which rice is cooked using water to which antifreeze protein has been added, a forming process (S106) in which the rice obtained in the rice cooking process is formed into sari balls, a freezing process (S107) in which the sari balls obtained in the forming process are frozen, and a glazing process (S108) in which a mist of liquid is sprayed onto the surface of the sari balls frozen in the freezing process to freeze them.

The invention relates to the technical field of anti-freezing concrete preparation, in particular to high-anti-freezing biomass concrete as well as a preparation method and application thereof. The invention provides application of cod antifreeze protein in preparation of high-antifreeze concrete. The cod antifreeze protein is added into the preparation of the antifreeze concrete, and hydrogen bonds formed by amino acids in the cod antifreeze protein and water / ice influence the icing temperature and icing morphology of a pore solution and reduce the formation of large ice crystals, so that the ice crystal pressure is reduced, and the antifreeze capability of the concrete is improved.