Method for extracting apoplast antifreeze proteins from winter rape roots

A technology of antifreeze protein and extraction method, which is applied to the preparation method of peptides, chemical instruments and methods, plant peptides, etc., and can solve the problem that there is no mature method for antifreeze protein in rapeseed
CN103333234AInactive Publication Date: 2013-10-02GANSU AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GANSU AGRI UNIV
Publication Date
2013-10-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method for extracting antifreeze proteins, in particular to a method for extracting apoplast antifreeze proteins from winter rape roots. According to the method, apoplast antifreeze proteins are successfully extracted from cold-induced winter rape roots. The method for extracting apoplast antifreeze proteins from winter rape roots is characterized by comprising the following steps: (1) sampling; (2) extracting a buffer solution for preparing; (3) slitting; (4) immersing; (5) vacuumizing; and (6) centrifuging. According to the method, apoplast antifreeze proteins are successfully and rapidly extracted from cold-induced winter rape roots, so that technical support is provided for further research of the cold resistance mechanism of winter rape.
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Description

technical field

[0001] The invention relates to a method for extracting antifreeze protein, in particular to a method for extracting antifreeze protein from winter rapeseed root apoplast. The method successfully extracts the apoplast antifreeze protein from cold-induced winter rapeseed root. Background technique

[0002] Low temperature is one of the main environmental factors affecting the normal growth and development of plants in northern my country. When plants encounter low temperature stress, a series of physiological and biochemical changes will occur in the body to adapt to low temperature stress, and at the same time they will synthesize and secrete a variety of proteins - low temperature induced proteins. Antifreeze proteins (AFPs) are a class of low-temperature-induced proteins, which can be produced in different organs such as roots, stems, and leaves of plants, and can also exist in callus under tissue culture conditions, suspension culture cells and suspension ...

Claims

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