The invention discloses a transaminase mutant and an application thereof. An amino acid sequence of the transaminase mutant is the amino acid sequence obtained by mutating the amino acid sequence shown as SEQ ID NO: 1, and the mutation includes at least one of the following mutation sites: 3rd, 5th, 8th, 25th, 32nd, 45th, 56th, 59th, 60th, 84th, 86th, 164th, 176th, 178th, 180th, 187th, 197th, 206th, 207th, 242th, 245th, 319th, 324th sites and the like; alternatively, the amino acid sequence of the transaminase mutant has the mutation site in the amino acid sequence in which the mutation is generated. The application of the mutant can increase the reaction rate, improves the stability of the enzyme, reduces the amount of the enzyme, and reduces the difficulty of the post-treatment, so thatthe transaminase mutant can be suitable for industrial production.