High-protein distiller's grain feed and preparation method thereof

A technology of high-protein wine and distiller's grains, applied in animal feed, animal feed, application, etc., can solve the problems of lack of animal protein, can only be used as roughage, and low degradation rate of crude fiber, so as to increase nutritional value and enzyme content Large, good enzyme stability

Inactive Publication Date: 2018-11-30
十堰真武酒业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are still some deficiencies in the existing microbial fermentation and enzymatic hydrolysis methods: First, the degradation mechanism of lignin is different from that of cellulose and hemicellulose. Some of them can only effectively degrade cellulose and hemicellulose. If only any one type of microorganism is selected to degrade crude fiber, a higher crude fiber degradation rate cannot be obtained. For example, if only cellulose and hemicellulose are selected If the cellulose is fermented by Trichoderma, the lignin in the rice husk cannot be effectively degraded, and the lignin binds to the cellulose and hemicellulose, thereby inhibiting the degradation of the cellulose and hemicellulose, making the crude The overall degradation rate of the fiber is low; secondly, there is no effective control of some influencing factors in the microbial fermentation and degradation process, such as the selection and ratio of carbon source and nitrogen source, degradation intermediate products and substrate metal ions and their concentration; finally, The high-protein feed produced by fermenting distiller's grains only contains plant-based protein, lacks animal-based protein, and has insufficient nutrition. It can only be used as roughage. It must be combined with other nutritious feed when feeding poultry and livestock.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A preparation method of high-protein distiller's grains feed, comprising the following steps:

[0031] (1) Pretreatment, heating the fresh distiller's grains at a temperature of 150°C to a moisture content of 23%, adding bran to the dried fermentation raw material as a carbon source, and the weight ratio of fermentation raw material to bran is 5: 1. Add ammonium salt as a quick-acting nitrogen source, and silkworm chrysalis powder with a weight equivalent to 12% of the weight of fresh distiller's grains as a late-acting nitrogen source, adjust the pH to 6.0, the temperature is 30°C, and the humidity is 82%, to obtain a distiller's grain fermentation medium, Sterilizing the fermentation medium;

[0032] (2) First-level degradation, inoculate the first strain into the sterilized fermentation medium, the first strain is Phanerochaete chrysosporium, the inoculation amount is 7%, and the oxygen flow rate in the early stage of fermentation is controlled at 7:2, controlled at...

Embodiment 2

[0035] A preparation method of high-protein distiller's grains feed, comprising the following steps:

[0036] (1) Pretreatment, after mixing fresh distiller's grains and fresh beer's grains at a ratio of 2:1, heat at 180°C to obtain fermentation raw materials with a moisture content of 20%, add bran to it, and ferment raw materials and bran The weight ratio of skin is 6:1, ammonium salt is added as the quick-acting nitrogen source and silkworm chrysalis powder whose weight is equivalent to 10% of the weight of fresh distiller's grains is used as the late-acting nitrogen source, the pH is adjusted to 6.5, the temperature is 28°C, and the humidity is 84%. , to obtain distiller's grains fermentation medium, which is sterilized;

[0037] (2) First-level degradation, inoculate the first strain into the sterilized fermentation medium, the first strain is Phanerochaete chrysosporium, the inoculation amount is 5%, and the oxygen flow rate in the early stage of fermentation is controll...

Embodiment 3

[0040] A preparation method of high-protein distiller's grains feed, characterized in that: comprising the following steps:

[0041] (1) Pretreatment, mix fresh distiller's grains, fresh beer's grains and sugarcane juice at a ratio of 12:4:1, heat at 160°C to obtain a fermentation material with a moisture content of 24%, and add bran to it , the weight ratio of fermentation raw materials and bran is 6:1, ammonium salt is added as the available nitrogen source, and silkworm chrysalis powder with a weight equivalent to 14% of the weight of fresh distiller's grains is used as the delayed nitrogen source, the pH is adjusted to 7.0, and the temperature is 32 ℃, humidity is 88%, obtain distiller's grains fermentation medium, and the fermentation medium is sterilized;

[0042] (2) First-level degradation, inoculate the first bacterial species into the sterilized fermentation medium, the first bacterial species are Pleurotus eryngii and Phanerochaete chrysosporium, and the inoculum am...

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PUM

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Abstract

The invention discloses a high-protein distiller's grain feed and a preparation method thereof. With distiller's grain and silkworm pupa powder as main materials, the preparation method for the high-protein distiller's grain feed utilizes a variety of strains ( such as phanerochaete chrysosporium, trichoderma reesei and aspergillus niger) to carry out step-by-step fermentation, and thereby lignin,cellulose and hemicellulose in distiller's grain are deeply degraded in succession. The invention effectively controls factors (such as choice and proportioning of carbon source and nitrogen source,oxygen input, intermediate degradation product, enzyme activator and inhibitor) in the process of microbial fermentation degradation, increasing the degradation rate of crude fibers, the utilization rate of distiller's grain and the feeding value of the distiller's grain feed. According to the high-protein distiller's grain feed prepared by the fermentation method disclosed by the invention, the crude fiber content is decreased by 12 to 15 percent, the crude protein content is increased by 25 to 28 percent, and the nutrition of the high-protein distiller's grain feed is closer to the nutritionof complete feed.

Description

technical field [0001] The invention relates to the technical field of animal feed, in particular to a high-protein distiller's grain feed and a preparation method thereof. Background technique [0002] Distiller's grains are the residues of sorghum, corn, wheat and other single raw materials or mixed raw materials after fermentation and distillation. They are feed raw materials with high nutritional value. [0003] Carbohydrates in liquor brewing raw materials are fermented into ethanol and then separated by distillation. The content of crude protein and crude fat in distiller's grains is higher than that of raw materials, the content of nitrogen-free extracts is correspondingly reduced, and the content of B vitamins is also lower. It can be added to the diet of poultry and livestock as roughage, but distiller's grains also contain a large amount of crude fiber, which mainly comes from the rice husk used as a filler in the brewing process of liquor. These crude fibers inclu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/16A23K10/38
CPCA23K10/12A23K10/16A23K10/38Y02P60/87
Inventor 朱上能阳建波刘相兰
Owner 十堰真武酒业有限公司
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