The application discloses an
exosome preparation for treating hypofunction of
ovary and a preparation method and application thereof, and belongs to the technical field of biological
medicine, and particularly relates to a preparation method of an
exosome preparation for treating hypofunction of
ovary, which comprises the following steps: taking perinatal stem cells, subculturing, obtaining domesticated perinatal stem cells, digesting, expanding and culturing to a density of 80%-85%, adding a culture medium for culturing, collecting supernatant, collecting supernatant for 3 times, combining, filtering through a 0.45 mu m PES filter membrane first, and then concentrating to obtain a concentrated solution; centrifuging, resuspending a precipitate, purifying through a
density gradient, obtaining an
exosome precipitate, and suspending the exosome precipitate in a
cryopreservation protective solution to obtain the exosome preparation. The application provides the exosome preparation for treating hypofunction of
ovary and the preparation method and application thereof, a safe, sufficient, high-purity and stable exosome preparation is prepared, and the
stem cell exosome for treating hypofunction of ovary is provided.