The invention relates to the technical field of modern breeding industry, and discloses a research method for degrading crude fibers by synergistically fermenting distillers' grains with artificial combined
flora, which comprises the following steps: S1, activating single
bacteria of the artificial combined
flora; s2, preparing an artificial combined
flora solution; s3,
vinasse pretreatment and screening; s4, preparing a
solid fermentation culture medium; s5,
inoculation and
solid fermentation; s6, monitoring the
fermentation process; s7, measuring a product after fermentation; and S8, evaluating the in-vitro digestibility. The three strains are combined in proportion to form an artificial combined flora to cooperatively ferment the
vinasse, so that the technical effects of efficiently degrading crude fibers in the
vinasse in a targeted manner and cooperatively improving the activity of a key degrading
enzyme so as to strengthen the overall degradation efficiency are achieved, and compared with the common technical scheme of single-strain fermentation or simple
mixed flora fermentation without a specific proportion in the prior art, the technical scheme has the advantages that the cost is reduced, and the production efficiency is improved. The defects that the crude
fiber degradation is not thorough, the
enzyme activity
secretion type is single, the
synergy among strains is poor, and the anti-enzymolysis structure of the crude
fiber in the vinasse is difficult to break through are overcome.