Sample bag for measuring feeder rumen organic substance, protein, neutral detergent fiber (NDF) and acid detergent fiber (ADF) degradation rate and application thereof

A degradation rate, organic matter technology, applied in the direction of removing certain components and weighing, etc., can solve the problems of poor repeatability of data results, low manual measurement efficiency, complicated operation steps, etc., to achieve high accuracy, good plastic sealing, and operation. simple steps

Inactive Publication Date: 2015-07-08
SHANDONG AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are some problems in the determination of the rumen degradation rate of ruminant feed by using the nylon bag method
Since nylon cannot withstand high temperature and strong acid, it is impossible to directly analyze the residue composition in the nylon bag. It is necess

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: the preparation of polyester fiber net bag: select imported high-quality polyester filament (polyethylene terephthalate, the diameter of filament is 34 μm) to weave into uniform polyester with aperture of 20 μm Ultra-fine mesh, made into a 6cm×8cm rectangular polyester mesh bag, with plastic seals on three sides and an opening on one side.

Embodiment 2

[0026] Embodiment 2: various processing weight loss and ash test of polyester fiber mesh bag:

[0027] After selecting 10 polyester fiber filter bags of Example 1 and weighing them, they were dried at 105° C. for 1 hour and weighed; after being boiled for 1 hour according to the ADF method, they were extracted with acetone and dried at 105° C. for 1 hour and weighed; Soak in 72% concentrated sulfuric acid solution at room temperature for 3 hours, take it out and wash it, dry it at 105°C for 1 hour and weigh it; determine whether there is weight loss after various treatments. Finally, put 10 blank polyester fiber filter bags, and carbonize the crucible with the filter bags on the electric furnace until there is no smoke. After carbonization, ash in a muffle furnace at 550°C for 2 hours, and cool to room temperature in a desiccator. weighing.

[0028] The results of the analysis experiment found that the average weight loss of each bag after drying at 105°C was 0.0003g, which c...

Embodiment 3

[0029] Embodiment 3: the application of polyester mesh bag in the determination of feed rumen organic matter, protein, neutral detergent fiber, acid detergent fiber degradation rate

[0030] 1) Fabrication of fiber mesh bags: Polyester fibers, namely polyethylene terephthalate monofilaments (polyethylene terephthalate, the diameter of the filaments are 34 μm) are woven into uniform bags with a pore size of 20.0 μm. The monofilament fiber screen cloth of above-mentioned monofilament fiber screen cloth is made into the rectangular screen bag of 4-6cm * 5-8cm again, and one side opening of screen bag.

[0031]2) After numbering and weighing the polyester net bags with a special marker, accurately weigh about 1-2g of the sample (1g for coarse feed, 2g for concentrated feed) and put it in the bag, and seal it with plastic. Make 48 bags of each sample, 10 kinds of samples can be made at one time, a total of 480 bags. Dry the sealed polyester mesh bag containing the sample in an ove...

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Abstract

The invention provides a sample bag for measuring feeder rumen organic substance, protein, NDF and ADF degradation rate and an application thereof. A certain amount of to-be-measured feed samples are put in sample bags to be put in the ruminant rumen after mechanical sealing, the feed samples are taken out after being digested in the ruminant rumen for certain time to be washed and dried, and the ruminant feeder rumen organic substance, protein, NDF and ADF degradation rate is measured by processing polyester mesh bags including residues. Compared with measuring of ruminant feeder rumen organic substance, protein, NDF and ADF degradation rate through a nylon bag method, the method has the advantages of being capable of performing batch processing, simple and rapid in operation step, good in repeatability, low in cost, simple in analysis method, high in data accuracy and the like and capable of providing a simple and efficient measuring method for ruminant feed nutritive value evaluation.

Description

Technical field: [0001] The invention relates to the field of feed nutritional value evaluation, in particular to a sample bag for measuring the degradation rate of feed rumen organic matter, protein, neutral detergent fiber and acid detergent fiber and an application thereof. Background technique [0002] The rumen is the first stomach of ruminants. There are a large number of microorganisms parasitic in the rumen. The feed eaten by ruminants first enters the rumen. In the rumen, the food is decomposed and fermented under the action of microorganisms, and the macromolecules in the feed are degraded. It is a small molecular substance, which is absorbed and utilized by animals or excreted from the body. Understanding the degradation rate of ruminant feed in the rumen is the basis for evaluating the nutritional value of feed to guide scientific feeding of ruminants. [0003] The degradation rate of rumen organic matter in ruminant feed can be used to evaluate the available en...

Claims

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Application Information

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IPC IPC(8): B65D30/06G01N5/04
Inventor 张崇玉曲绪仙杨在宾杨维仁姜淑贞张桂国王保哲
Owner SHANDONG AGRICULTURAL UNIVERSITY
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