The invention belongs to the field of molecular biology, and particularly relates to a nasopharyngeal carcinoma microRNA detection kit. A purpose of the present invention is to solve the problems of poor stability, poor reproducibility, low specificity and easy false positive generation of the existing nasopharyngeal carcinoma microRNA detection kit. The invention provides the nasopharyngeal carcinoma microRNA detection kit, which comprises a probe composition, wherein the probe composition comprises a capture probe bound with a target microRNA and a signal amplification composition, and the target microRNA is one or a plurality of microRNA selected from hsa-miR-17-5p, hsa-miR-20a-5p, hsa-miR-29c-3p, hsa-miR-223-3p, hsa-miR-34b-3p, hsa-miR-34c-5p, hsa-miR-212-3p, hsa-miR-216a-5p, hsa-miR-217, hsa-miR-151a-3p, and hsa-miR-192-5p. The nasopharyngeal carcinoma microRNA detection kit of the present invention has advantages of good stability, good repeatability, high specificity, less false positive generation, and high sensitivity.