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Nasopharyngeal carcinoma microRNA detection kit

A technology of hsa-mir-34b-3p and hsa-mir-34c-5p, which is applied in the field of molecular biology, can solve the problems of low specificity, weak repeatability and poor stability, and achieves high specificity and good repeatability. , good stability

Active Publication Date: 2017-05-10
SUREXAM BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the current in situ hybridization technology, there are still many problems in the kits used for the multiple parallel detection of nasopharyngeal carcinoma microRNA, such as: poor stability, weak repeatability, low specificity, prone to false positives and sensitivity low disadvantage

Method used

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  • Nasopharyngeal carcinoma microRNA detection kit
  • Nasopharyngeal carcinoma microRNA detection kit
  • Nasopharyngeal carcinoma microRNA detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] This example provides a method for preparing a capture probe. The base sequence of the capture probe designed in this example is, from the 5' end to the 3' end, the specific sequence P1 that binds to the target microRNA to be detected, the spacer arm sequence, P2 sequence.

[0021] The spacer sequence can separate the P2 sequence of the capture probe from the target microRNA to be detected. By setting the spacer sequence of an appropriate length inside the probe, it can reduce steric hindrance, improve the efficiency and specificity of the hybridization reaction sex. The spacer sequence of the capture probe of the present invention is preferably 5 Ts.

[0022] This example is aimed at hsa-miR-17-5p, hsa-miR-20a-5p, hsa-miR-29c-3p, hsa-miR-223-3p, hsa-miR-34b-3p, hsa-miR-34c- 5p, hsa-miR-212-3p, hsa-miR-216a-5p, hsa-miR-217, hsa-miR-151a-3p and hsa-miR-192-5p designed capture probes, see Table 1, Table 1 2:

[0023] Table 1 P1 sequence of capture probe

[0024]

...

Embodiment 2

[0029] This embodiment provides the preparation method of the signal amplifying component, the signal amplifying component obtained by the preparation method described in this embodiment is selected from: the first signal amplifying component, the second signal amplifying component and the third signal amplifying component Any one; the first signal amplification component includes a primary signal amplification probe, and the 3' end of the first signal amplification component is also modified with a fluorescent group; the second signal amplification component is a primary signal amplification probe and secondary signal amplification probe, the 3' end of the second signal amplification component is also modified with a fluorescent group; the third signal amplification component is a primary signal amplification probe, a secondary signal amplification In the amplification probe and the tertiary signal amplification probe, the 3' end of the third signal amplification component is ...

Embodiment 3

[0056] This embodiment provides a preparation method of nasopharyngeal carcinoma microRNA detection kit, said nasopharyngeal carcinoma microRNA detection kit comprises: the probe component prepared in Example 1 and the signal amplification component prepared in Example 2 .

[0057] The composition of a nasopharyngeal carcinoma microRNA detection kit designed in this embodiment is shown in Table 7.

[0058] Table 7 A nasopharyngeal carcinoma microRNA detection kit (table number is SEQ ID NO.)

[0059]

[0060]

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Abstract

The invention belongs to the field of molecular biology, and particularly relates to a nasopharyngeal carcinoma microRNA detection kit. A purpose of the present invention is to solve the problems of poor stability, poor reproducibility, low specificity and easy false positive generation of the existing nasopharyngeal carcinoma microRNA detection kit. The invention provides the nasopharyngeal carcinoma microRNA detection kit, which comprises a probe composition, wherein the probe composition comprises a capture probe bound with a target microRNA and a signal amplification composition, and the target microRNA is one or a plurality of microRNA selected from hsa-miR-17-5p, hsa-miR-20a-5p, hsa-miR-29c-3p, hsa-miR-223-3p, hsa-miR-34b-3p, hsa-miR-34c-5p, hsa-miR-212-3p, hsa-miR-216a-5p, hsa-miR-217, hsa-miR-151a-3p, and hsa-miR-192-5p. The nasopharyngeal carcinoma microRNA detection kit of the present invention has advantages of good stability, good repeatability, high specificity, less false positive generation, and high sensitivity.

Description

technical field [0001] The invention belongs to the field of molecular biology, in particular to a nasopharyngeal carcinoma microRNA detection kit. Background technique [0002] MicroRNAs (miRNAs) are a class of endogenous non-coding RNAs with regulatory functions found in eukaryotes, with a size of about 20-25 nucleotides. Mature miRNAs are produced by a series of nuclease cleavage processes from longer primary transcripts, and then assembled into the RNA-induced silencing complex (RNA-induced silencing complex, RISC) through complementary base pairing. Recognize the target miRNA, and guide the silencing complex to degrade the target miRNA or repress the translation of the target miRNA according to the degree of complementarity. Recent studies have shown that miRNAs are involved in a wide variety of regulatory pathways, including development, viral defense, hematopoietic processes, organ formation, cell proliferation and apoptosis, fat metabolism, and more. In recent year...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 刘苏燕吴诗扬董艳
Owner SUREXAM BIO TECH
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