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Application of carbon nanodots as water-soluble ratiometric fluorescent probe

A technology of carbon nano-dots and fluorescent probes, applied in nano-carbon, nano-technology, nano-technology, etc., can solve the problems of high cost, weak luminescence stability, toxicity, etc., and achieve a simple method, high sensitivity, and strong accuracy Effect

Active Publication Date: 2013-02-27
CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the technical problems of high cost, weak luminescence stability, and potential toxicity of the existing ratiometric fluorescent probes, the present invention provides an application of carbon nanodots as a water-soluble ratiometric fluorescent probe

Method used

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  • Application of carbon nanodots as water-soluble ratiometric fluorescent probe

Examples

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Effect test

Embodiment 1

[0054] combine Figure 1-3 Illustrative Example 1

[0055] Dissolve 0.3mg of carbon nano-dots in 3ml of neutral pure water, respectively, at fixed excitation wavelengths of 360nm, 380nm and 400nm, and 25°C, perform fluorescence spectrum detection on the aqueous solution of carbon nano-dots to detect the blue color of the aqueous solution of carbon nano-dots Intensities of the maximum fluorescence emission peak and the maximum green fluorescence emission peak, calculate the ratio value of the intensity of the maximum blue fluorescence emission peak and the maximum green fluorescence emission peak intensity.

[0056] figure 1 It is the fluorescence spectrum diagram of the aqueous solution of carbon nano-dots in Example 1 of the present invention under different excitation wavelengths, curve 1 is the fluorescence spectrum under 360nm excitation, curve 2 is the fluorescence spectrum under 380nm excitation, and curve 3 is the fluorescence under 400nm excitation spectrum; from fi...

Embodiment 2

[0061] combine Figure 4 and Figure 5 Illustrative Example 2

[0062] Application of carbon nanodots as water-soluble ratiometric fluorescent probes for temperature detection: 0.3mg carbon nanodots were dissolved in 3ml neutral pure water, and the excitation wavelength was fixed at 380nm, respectively at 10°C, 25°C, 38°C, 48°C °C, 53 °C, 58 °C, 63 °C, 73 °C, 78 °C and 83 °C, the fluorescence spectrum detection is carried out on the aqueous solution dissolved with carbon nano-dots, and the blue maximum fluorescence emission peak and the green maximum fluorescence emission peak intensity are measured, Calculate the ratio value of the intensity of the blue maximum fluorescence emission peak and the green maximum fluorescence emission peak, and then achieve the detection of temperature.

[0063] Figure 4 It is the normalized fluorescence emission spectrum at the green maximum fluorescence emission peak of the aqueous solution in which carbon nano-dots are dissolved in Example...

Embodiment 3

[0066] combine Image 6 and Figure 7 Illustrative Example 3

[0067] Application of carbon nanodots as water-soluble ratiometric fluorescent probes to detect ethanol concentration in aqueous ethanol solutions:

[0068] Use ethanol and pure water to prepare ethanol aqueous solutions with ethanol volume percentages of 0, 0.05, 0.15, 0.20, 0.25, 0.38, 0.50, 0.63, 0.72, 0.88, 0.94 and 0.98, respectively, and dissolve 0.3mg of carbon nanodots to 3ml In the above-mentioned ethanol aqueous solution, the fixed excitation wavelength is 380nm, and at 25°C, the above-mentioned ethanol aqueous solution in which carbon nano-dots are dissolved is respectively subjected to fluorescence spectrum detection, and the intensity of the blue maximum fluorescence emission peak and the green maximum fluorescence emission peak are measured, and the blue The ratio value of the intensity of the maximum color fluorescence emission peak and the intensity of the maximum green fluorescence emission peak ...

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Abstract

The invention discloses an application of carbon nanodots as a water-soluble ratiometric fluorescent probe and solves the problems that the existing ratiometric fluorescent probes are high in preparation cost, poor in luminescent stability and provided with potential toxicity. According to the application, the carbon nanodots are dissolved in a solution to be measured, an excitation wavelength is fixed at 360-400nm,the solution to be measured dissolved with the carbon nanodots is subjected to fluorescence spectrum detection at a fixed temperature, intensities of a blue maximum fluorescence-emission peak and a green maximum fluorescence-emission peak are measured, the ratios of the blue maximum fluorescence-emission peak intensity and the green maximum fluorescence-emission peak intensity are calculated, so that the detection of the objective to be measured is achieved. The application has the advantages of being low in cost, good in luminescent stability, non-toxic, high in sensitivity and capable of achieving detection for temperature, pH of water solution, ethanol concentration in ethanol water and Fe3+ concentration in Fe3+-containing neutral water solution simply, accurately and quickly.

Description

technical field [0001] The invention belongs to the field of nanometer material science, and in particular relates to the application of a carbon nanometer point as a water-soluble ratio-type fluorescent probe. Background technique [0002] Fluorescent probes have important applications in the fields of life sciences and environmental detection. Existing fluorescent probes mainly use the fluorescence intensity of a single fluorophore as a characterization parameter. Although the operation is simple, the accuracy of the results is easily affected by the concentration of the fluorophore itself, instrument factors (such as fluctuations in the intensity of the excitation light source, instrument sensitivity) and so on. These factors will directly lead to a large error between the measurement result and the true value. Fluorescence ratio technology can better solve the above-mentioned problems existing in the existing fluorescence characterization. The ratiometric fluorescent ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B31/02B82Y30/00G01N21/64C01B32/15
Inventor 曲松楠刘星元
Owner CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
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