SSR (simple sequence repeat) markers on No.3 chromosome, closely linked to RBSDV (rice black-streaked dwarf virus) resistant QTL (quantitative trait locus) and application thereof

A rice black streak dwarfing and resistance technology, which is applied in the detection/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve problems such as interference, complex genetic mechanism, and influence on the reproducibility of results, and achieve accelerated breeding process, simplified selection methods, and improved breeding efficiency

Active Publication Date: 2014-02-05
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
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AI Technical Summary

Problems solved by technology

This suggests that there may be a differential mechanism of rice black-streaked dwarf resistance in rice resources from different sources, and the genetic mechanism is relatively complex
[0003] The field identification method of rice black-streaked dwarf disease-affected areas is convenient and quick, but the reproducibility of the results is affected by factors such as environmental conditions, annual and inter-regional differences in the occurrence of SBPH (incidence and infection rate), resulting in The results of resistance identification among different studies are very different. In addition, the field identification in severely diseased areas is very susceptible to the interference of rice stripe leaf blight transmitted by SBPH at the same time.
The artificial inoculation identification method (Journal of Plant Protection, 2011, 38(4): 301-305) is a resistance identification carried out under controlled conditions, and is used to evaluate the resistance level of rice varieties to rice black-streaked dwarf disease , in addition to overcoming the two problems that are difficult to avoid in field identification, it can also extend the identification time, but because RBSDV is not transmitted by SBPH eggs, this method is relatively time-consuming and laborious

Method used

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  • SSR (simple sequence repeat) markers on No.3 chromosome, closely linked to RBSDV (rice black-streaked dwarf virus) resistant QTL (quantitative trait locus) and application thereof
  • SSR (simple sequence repeat) markers on No.3 chromosome, closely linked to RBSDV (rice black-streaked dwarf virus) resistant QTL (quantitative trait locus) and application thereof
  • SSR (simple sequence repeat) markers on No.3 chromosome, closely linked to RBSDV (rice black-streaked dwarf virus) resistant QTL (quantitative trait locus) and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1, Acquisition of Molecular Markers Tightly Linked to Major QTL for Rice Black-streaked Dwarf Resistance

[0020] 1. Plant material

[0021] In 2008, the parents Huaidao 5 and Tetebo were planted in the field of Jiangsu Academy of Agricultural Sciences, and hybrid F 1 , next year F 1 F 2 Seeds, propagated F in Hainan in 2009 2 group, as a mapping group. f 2 Individual plant number, parent and hybrid F at tillering stage 1 and F 2 Some leaves of each individual plant in the population were stored in a -70°C refrigerator for SSR analysis, F 2 Harvest the individual plants of the population for phenotypic identification. In 2010, the main susceptible cultivar Huaidao 5 and the resistant cultivars Tetebo and F 1 and F 2:3 The family was identified by artificial inoculation (Journal of Plant Protection, 2011, 38(4): 301-305).

[0022] 2. Screening of toxin-transmitting mediators

[0023] Collect rice diseased plants showing RBSDV symptoms (Zhejiang Agricu...

Embodiment 2

[0061] Example 2, the application of the molecular markers closely linked to the rice black-streaked dwarf resistance QTL on chromosome 3 in the offspring of the hybrid combination with Huaidao 5 and Tetebo as parents

[0062] The SSR marker closely linked to the rice black-streaked dwarf resistance QTL obtained on chromosome 3, the F 2:3 Part of the individual plants in the family were predicted for resistance to rice black-streaked dwarf disease, the DNA of each individual plant was extracted, and then PCR amplification analysis was performed with the primers of SSR markers RM5626 and RM7097, and the band type analysis was used to determine whether there were corresponding markers , the presence of the marker indicates that the line has reached the level of resistance to black-streaked dwarf disease, and the absence of the marker indicates that the line is susceptible to the disease. Then, the actual resistance to black-streaked dwarf disease of the tested lines was measured...

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Abstract

The invention discloses molecular markers closely linked to an RBSDV (rice black-streaked dwarf virus) major QTL (quantitative trait locus). The essence of the invention is that according to the linkage and segregation rules, the F2 population of the hybrid generation of Tetep and RBSDV susceptible varieties is taken as the mapping population and the SSR (simple sequence repeat) markers RM5626 and RM7097 on the No.3 chromosome, which are closely linked to the RBSDV resistant major QTL are obtained through analysis of linkage between the results of evaluation of disease resistance and the SSR marker data. Whether a plant has resistance to RBSDV can be judged by detecting the SSR marker banding pattern of the single rice plant of the hybrid combination generation of Tetep and derived varieties (lines) thereof and the RBSDV susceptible varieties. After the SSR markers are applied to assistant selection breeding of the RBSDV resistant varieties of Tetep and derived varieties (lines) thereof/RBSDV susceptible varieties, the defects of poor stability and repeatability of RBSDV resistance evaluation, time and labor waste, high technical threshold and the like in conventional breeding can be overcome and the results can conduce to simplifying the selection method and improving the breeding efficiency and then accelerating the breeding process of RBSDV resistant varieties.

Description

Technical field: [0001] The invention relates to molecular markers RM5626 and RM7097 on chromosome 3 closely linked with rice black-streaked dwarf resistance main effect QTL, which can be applied to molecular marker-assisted breeding and belong to the fields of rice disease-resistant breeding and molecular biology. Background technique: [0002] Rice black-streaked dwarf disease, the pathogen of which is rice black-streaked dwavf virus (RBSDV), is a vicious viral disease that is persistently transmitted non-ovially by the small planthopper. Most of the diseased rice plants infected with RBSDV can't head, and the yield is seriously reduced. In addition to rice, it also harms crops such as wheat, corn, barley and sorghum. In the late 1960s and 1990s, the disease broke out in North China, East China and other places. In recent years, the widespread planting of susceptible varieties, the promotion of rice-wheat rotation, and changes in environmental conditions such as winter cli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 周彤杜琳琳周益军王英王跞姣兰莹孙枫
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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