388 results about "Rice plant" patented technology

The invention relates to the field of rice gene engineering, and particularly provides OsSULTR2; the invention also discloses application of the 2 protein and the coding gene thereof in regulating and controlling the salt tolerance of rice. The protein meets the following conditions: B1) a protein with an amino acid sequence of SEQ ID NO.1; and B2) a fusion protein with the same function obtained by connecting a tag to the N end and / or C end of B1). The method is used for detecting OsSULTR2; the salt stress phenotype identification in the seedling stage is carried out on the transgenic rice with the gene knockout 1, 2, and the result shows that when the gene segment is deleted, the salt stress tolerance of the rice is improved, and the function and the application way of the gene are proved. Therefore, the OsSULTR2 of the present invention; the 2 protein and the coding gene thereof can regulate and control the salt tolerance of rice, and have important significance for cultivating salt-tolerant transgenic rice.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an SCRE10 gene for improving rice disease resistance and application of the SCRE10 gene, the base sequence of the SCRE10 gene is shown as SEQ ID NO.1, and the amino acid sequence of the SCRE10 gene is shown as SEQ ID NO.2. Through construction of a dexamethasone induced expression SCRE10 transgenic rice plant, it is found that the transgenic rice can significantly induce PR gene expression and active oxygen outbreak, and the resistance of rice to false smut and bacterial leaf blight can be improved. The invention proves that heterologous inducible expression of the Ustilaginoidea virens SCRE10 gene has the function of positively regulating the disease resistance of the rice, and the SCRE10 gene can be used for improving the disease resistance of the rice, which is of great significance to the creation of disease-resistant germplasm of the rice.

The invention relates to the technical field of gene engineering, and discloses an METS2 gene for regulating and controlling the grain shape of rice, a protein and application of the METS2 gene, and the nucleotide sequence of the METS2 gene is shown as SEQ ID NO.1. The invention further discloses application of the METS2 gene in regulation and control of the rice grain shape. According to the METS2 gene for regulating and controlling the rice grain shape, the protein and the application of the METS2 gene, the METS2 gene is overexpressed in a rice plant, short-grain-shaped rice can be obtained, the short-grain-shaped rice is not prone to breakage in the processing process, and the quality of a finished product is high; a basis is provided for further clarification of the molecular mechanism of the rice grain shape, and new gene resources and breeding resources are provided for rice breeding.

The invention provides a rice variable rate fertilization method and device, electronic equipment and a storage medium, and relates to the technical field of agricultural intelligent information process.The method comprises the steps that non-rice plant interferents in a multispectral remote sensing image are removed, and a rice plant canopy mask is generated; based on the rice plant canopy mask, multi-dimensional features of the rice plant canopy are extracted and fused, and a fused growth feature vector is obtained; grading the rice growth vigor according to the fused growth vigor feature vector through a growth vigor grade prediction model to obtain a predicted growth vigor grade; performing standardization processing on the predicted growth vigor level to obtain a standardized growth vigor level; determining a nitrogen fertilizer adjustment factor based on the standardized growth vigor grade; and according to the nitrogen fertilizer adjustment factor, adjusting a standard nitrogen application amount to obtain a recommended nitrogen application amount. According to the invention, accurate recommendation of the nitrogen application amount in the rice planting process can be realized, so that accurate fertilization is realized.

The invention belongs to the technical field of plant gene engineering, and particularly relates to an OsNUC1 gene and application of a gene mutant constructed by the OsNUC1 gene to regulation and control of rice plant height, leaf length and panicle type. The regulation is to regulate the plant height and leaf growth of the rice and influence the shape of the ear part. Compared with the prior art, the OsNUC1 gene mutant has the following advantages: (1) the OsNUC1 is directionally edited by utilizing a CRISPR / Cas9 gene editing technology, an OsNUC1 gene mutant rice material is obtained, the biological phenotype of the OsNUC1 gene mutant rice material is investigated, the phenotype investigation on T1-generation seedlings of the OsNUC1 shows that the plant heights and the leaf lengths of one-week-old and two-week-old seedlings of the mutant are obviously higher than those of wild seedlings, the leaf widths have no obvious difference, and the OsNUC1 gene mutant rice material has a good application prospect; the result shows that the OsNUC1 can regulate the plant height and the leaf growth of the rice and influence the shape of the ear part. (2) it is proved that the nucleolin gene OsNUC1 is negatively regulated by a transcription factor OsARF7, and the phenotypes such as the plant height, the leaf length, the grain length and the grain width of the OsNUC1 / OsARF7 mutant are more obvious than those of the OsNUC1 mutant. The invention further proves the application of the OsNUC1 gene in regulation and control of rice plant height and leaf growth.

The invention relates to the field of intelligent agriculture, in particular to a high-salt-tolerance rice planting management system based on image recognition, which comprises a data acquisition module, an image processing module, a growth condition detection module and an analysis management module, through the computer vision technology, close planting degree guidance and saline-alkaline degree control are intelligently provided for high-salt-tolerance rice planting, the management efficiency is improved, and the rice yield and quality are improved; according to the method, a Transformer coding method based on a window mechanism and global optimization is adopted, the recognition accuracy of the growth state of a rice plant is improved, the accuracy of analysis of the relationship between the growth condition of the rice and the salt and alkali content and the close planting degree of the rice is improved, and accurate guidance is provided for increasing the yield and quality of the rice; according to the method, a UNet network based on Mama block optimization is adopted, the influence of a field background on rice image feature extraction is removed, and the accuracy of rice growth condition-saline and alkaline content-rice close planting degree relation analysis is improved.

The invention belongs to the technical field of biology, and discloses a rice receptor-like kinase OsLTK1 and application thereof in resistance of magnaporthe oryzae. It is found for the first time that receptor-like kinase OsLTK1 positively regulates the resistance of rice to magnaporthe oryzae. The research shows that the transgenic plant of the OsLTK1 gene overexpressed in the rice has obvious resistance to the magnaporthe oryzae. Therefore, the OsLTK1 gene can be used for cultivating rice blast resistant rice varieties, and has great application value.

The present invention relates to methods for controlling or preventing infestation of a rice plant by the phytopathogenic microorganism Gibberella fujikuroi, comprising applying to a crop of rice plants, the locus thereof, or propagation material thereof, a compound according to formula (I) wherein R1, R2, R3, R4, R5, Y, A, B are as defined herein.

The utility model belongs to the technical field of rice planting, and particularly relates to a lodging-resistant device for rice planting, which comprises a support column, a winding piece, a locking piece and an inhaul cable, a winding roller of the winding piece is rotatably connected to the upper end of the support column through a bearing, and a driving rod is arranged at the position of a first check block arranged on the upper portion of the winding roller. A locking piece is arranged on a supporting column on the lower portion of the winding roller, a sliding sleeve is connected to a fixing sleeve of the locking piece in a sliding mode, and a stop lever fixedly connected to the upper end of the sliding sleeve abuts against a second stop block arranged on the lower portion of the winding roller. The supporting columns are used for fixing the winding rollers for winding the inhaul cables to the two ends of a row or a row of plants, the ends of the two inhaul cables are fixedly connected together, the inhaul cables are arranged on one sides of the rice plants in a lap joint mode, the rice plants are effectively blocked and supported, and the rice plants are prevented from lodging on the ground under the action of external factors such as wind and rain. The rice lodging-resistant device is simple in structure, can effectively prevent rice plants from lodging, and is convenient to use.

The invention discloses a rice growth image real-time acquisition method. The method comprises the following steps: real-time data acquisition; processing data; carrying out data visualization and analysis; according to the invention, through collection and fusion of multi-dimensional data, in combination with image analysis and a machine learning algorithm, key characteristics of rice growth are extracted; field environment data and rice plant height information are collected in real time, time sequence characteristics of environment parameters and plant height parameters are extracted based on a long-short-term memory network model, a long-term dependency relationship is captured, and accurate prediction of the rice plant height is achieved. A prediction result can be visually analyzed through a thermodynamic diagram and the like, and the growth condition of the rice is visually displayed; through a large number of trained long-short-term memory network models, precise prediction of the rice plant height can be realized, and during subsequent implementation of acquisition of rice growth images, dependence on manual observation or regular shooting is not needed, so that real-time predictability of rice growth is improved.

The invention discloses an application of a rice heat shock protein HSP101 and a coding gene thereof in rice heat-resistant breeding, and belongs to the technical field of gene engineering. The rice heat shock protein HSP101 is identified, the amino acid sequence of the rice heat shock protein HSP101 is shown as SEQ ID NO. 2, and the nucleotide sequence of the coding gene HSP101 is shown as SEQ ID NO. 1. The invention provides an application of a rice heat shock protein HSP101 and a coding gene HSP101 in heat-resistant breeding. Transgenic experiment results show that the survival rate of a rice plant overexpressed with the HSP101 gene after high temperature stress is significantly higher than that of a control plant, that is, the overexpressed HSP101 gene significantly improves the tolerance of rice to high temperature, and the HSP101 gene positively regulates the heat resistance of rice. The invention lays a foundation for stress-resistant breeding of rice, and has great economic benefits and wide application prospects for rice production.

The invention discloses a rice irrigation device and method based on layered soil moisture content sensing. The device comprises a water storage tank, a rainwater collector, a water conveying pipe, a table top, a water pump, a water outlet pipe, a hydraulic rotary spraying rod, an electromagnetic valve, a control box, a water inlet pipe, a layered soil sensor and a weather receiver. The method comprises the following steps: monitoring soil moisture content data of different depths in a root system area in real time by adopting a layered soil sensor, and collecting environmental meteorological parameters by utilizing a meteorological fusion device; the control box adopts a multi-factor dynamic weight decision-making algorithm to comprehensively analyze the soil moisture content and meteorological conditions and generate an intelligent irrigation decision-making instruction; accurate irrigation operation is achieved by controlling executing mechanisms such as a water pump, a hydraulic rotary spraying rod and an electromagnetic valve. By using the device, the irrigation amount and the irrigation time are accurately controlled, and the differentiated requirements of rice for water in different growth stages are met. The method can be widely applied to the field of intelligent agriculture.

The invention relates to application of rice genes SPPL1 and / or SPPL2 in improving high-temperature resistance of rice. Specifically, rice genes SPPL1 and / or SPPL2 participating in positive regulation and control of high-temperature stress resistance are cloned in rice, so that the rice genes SPPL1 and / or SPPL2 are overexpressed, and compared with a wild plant, the rice gene SPPL1 and / or SPPL2 overexpressed plant shows high-temperature-resistant characteristics, and agronomic characters (such as maturing rate, thousand seed weight, single-plant yield, plot yield and the like) are remarkably improved. Through cloning and discovery of new functions of the rice gene SPPL1 and / or SPPL2, a new gene target and a new resource are provided for improving high-temperature resistance genetic breeding of rice.

As a main grain crop in the world, the yield and quality of rice are often threatened by bacterial leaf blight and rice blast. According to the invention, a recombinant expression vector for expressing STTM-miR169d / p is constructed by starting from regulation and control of the expression quantity of miRNA through an STTM technology. By adopting an agrobacterium tumefaciens-mediated rice protoplast transformation technology, a rice plant containing the STTM-miR169d / p transgenic fragment is obtained. Through field inoculation of xanthomonas oryzae pv. Oryzae and inoculation of rice blast spores on in-vitro leaves, it is verified that the STTM-miR169d / p transgenic rice has significantly improved resistance to xanthomonas oryzae pv. Oryzae and rice blast compared with wild type rice. The invention not only provides a new germplasm resource for cultivating disease-resistant rice, but also provides a new thought for regulating and controlling the disease resistance of rice from the aspect of miRNA.

The invention relates to the technical field of rice phenotype extraction, and discloses a rice fine phenotype extraction method based on three-dimensional vision, a terminal and a storage medium. The method comprises the following steps: acquiring a multi-view image of a rice plant by using a depth camera, and performing camera attitude estimation on the multi-view enhanced image to obtain camera internal and external parameters corresponding to each view; constructing a neural radiation field network, training the neural radiation field network by taking the enhanced image, the contour mask and internal and external parameters of the camera as input, and learning color and density distribution of a scene by utilizing a volume rendering technology; extracting surface points based on a mixed threshold strategy by using the trained neural radiation field network, and generating a rice three-dimensional point cloud; inputting the rice three-dimensional point cloud into a point cloud instance segmentation network, and outputting an instance mask containing each organ of the rice and a segmentation result of a three-dimensional bounding box; and counting phenotypic parameters of the rice according to a segmentation result. According to the method, the fine instance segmentation of the high-density point cloud is realized while the reconstruction precision is ensured, and the phenotypic analysis of the spike fraction is supported.

The invention discloses a Chimonobambusa chensinensis CuCCoAOMT1 gene as well as a protein and application thereof, belongs to the technical field of plant genetic engineering, and particularly relates to a Chimonobambusa chensinensis caffeoyl-coenzyme A-O-methyltransferase CuCCoAOMT1 gene, and a nucleotide sequence of the CuCCoAOMT1 gene is shown as SEQ ID NO.1. The invention further discloses a preparation method of the CuCCoAOMT1 gene. The invention further discloses application of the CuCCoAOMT1 gene in regulation and control of the drought resistance of gramineous plants. By overexpressing the caffeoyl-coenzyme A-O-methyltransferase CuCCoAOMT1 gene in the rice, the lignin content in the rice can be effectively increased, the thickening of rice cell walls is promoted, the drought resistance of rice plants is improved, and a new gene resource is provided for plant material property improvement gene engineering.

The invention discloses a rice blast prevention and control and fertilizer efficiency improvement dual-objective optimization method, and relates to the technical field of agricultural informatics, and the method comprises the following steps: S1, data collection: obtaining rice field environment parameters, rice plant physiological parameters and pathogenic bacteria infection pressure indexes in real time; s2, constructing a double-objective collaborative optimization model: based on the data in the step S1, establishing a dynamic decision-making model which takes the maximization of the fertilizer efficiency utilization rate and the minimization of the rice blast occurrence risk as a combined objective. According to the method, a dynamic decision-making model taking maximization of the nitrogen fertilizer utilization rate and minimization of the rice blast severity as a combined target is constructed, the nitrogen fertilizer application amount, the bactericide type and the time sequence are subjected to collaborative optimization, a weight coefficient is introduced into the model, the target priority is dynamically adjusted according to the growth period, and the nitrogen fertilizer application amount is restrained in an agronomic safety interval; diseases caused by excessive application of nitrogen are avoided, positive correlation between the nitrogen fertilizer and the diseases is reduced from the source, pesticide dependence is reduced, and double improvement of the control effect and the fertilizer effect is achieved.

The invention discloses a heavy metal blocking agent special for rice and a method for reducing the cadmium content of rice grains by using the heavy metal blocking agent, and belongs to the technical field of agricultural environment protection and polluted soil remediation. The heavy metal blocking agent special for rice comprises the following raw materials: 20-30% of molybdenum disulfide, 10-20% of diammonium hydrogen phosphate, 5-10% of disodium magnesium ethylene diamine tetraacetate, 5-10% of zinc ethylene diamine tetraacetate, 10-20% of a silicon fertilizer, 1-2% of a cosolvent, 1-2% of a solubilizer, 1-2% of ethanol and the balance of water. The special heavy metal blocking agent for the rice can effectively inhibit heavy metal cadmium from being transferred into rice grains, obviously reduce the cadmium content of the rice grains, obviously improve the yield of the rice grains, and can obviously reduce the cadmium content in the rice and ensure that the cadmium content in the rice grains meets the national food hygiene standard when being applied to the rice planted in a rice field with soil polluted by cadmium.

The application provides a method for increasing the length of a main panicle of rice, the number of secondary branches of the main panicle, the number of grains on the main panicle of rice and / or improving the yield of rice, which comprises overexpressing a cystathionine beta-lyase gene OsCBL in rice by using a recombinant vector containing a CDS coding sequence of the cystathionine beta-lyase gene OsCBL as shown in SEQ ID NO: 2 to obtain a transgenic plant. The transgenic plant obtained by the method has an increased number of grains per panicle and an increased yield per plant compared with normal plants. The method can be used for treating rice plants to improve the yield of rice and provides a new gene resource and a new idea for breeding rice with increased yield.

The application discloses a gene marker for identifying the sensitivity of rice flowering to sunlight, which comprises SNP site sequences selected from the group consisting of SEQ ID NOs:1-3 of an Hd1 gene and SEQ ID NOs:4-5 of a Ghd8 gene. The gene marker can be used for identifying the sensitivity of rice flowering to sunlight, and effectively guides the rapid breeding of rice varieties.

The invention provides a nutrient solution and application thereof in prevention and treatment of crop sheath blight, and belongs to the technical field of rice planting. The nutrient solution provided by the invention contains cysteine with a certain concentration, after the nutrient solution is applied to rice in the trefoil stage and cultivated to the whole growth period, the resistance of the rice to sheath blight can be improved, and meanwhile, the content of chlorophyll, soluble sugar, soluble protein, SOD, POD, MDA and CAT in rice plants is remarkably increased. The nutrient solution provided by the invention can effectively solve the problem of poor sheath blight disease resistance of rice under conventional planting conditions, and has a wide application prospect.

A rice direct seeding seed coating method with iron powder as main raw material, comprising the following steps: 1) weighing rice seeds and pouring into a coating machine, carrying out spiral mixing, spraying water on the surface of the rice seeds in the coating machine to make the surface of the rice seeds evenly wet; 2) adding raw materials to the rice seeds in the coating machine for at least two times, adding sodium carboxymethyl cellulose, calcium sulfate powder, iron powder and calcium peroxide powder in sequence in steps each time, and finally adding a fungicide; the raw material dosages are based on the weight of the rice seeds: sodium carboxymethyl cellulose 1.5-3%, calcium sulfate CaSO4·2H2O 2-6%, calcium peroxide CaO2 4-8%, iron powder 35-45%, and the fungicide 0.1-0.4%, and the water addition amount is not more than 30%; 3) spreading the coated rice seeds into a container and drying under ventilation. The present application adopts iron powder to coat the rice seeds for direct seeding, without seed disinfection, with deeper seed fall depth, improved seed germination rate, enhanced rice plant lodging resistance, and high-efficiency weed control by direct seeding in water.

Provided are a rice mutant gene and the use thereof in regulating the plant height of rice. The rice mutant gene at least has an allele mutation, and the allele mutation is the mutation into Asn from Asp at the 377th site in an amino acid sequence shown as SEQ ID No. 26. The mutant gene can effectively regulate and control the rice plant height, and has good application potential in plant architecture breeding of rice varieties.

The invention relates to the technical field of plant breeding, in particular to a mutant of an FZP gene promoter and application of the mutant. Compared with a wild type rice FZP gene promoter, the mutant has any one of the following mutations: (1) 5bp is deleted from 3995bp to 3989bp at the upstream of an initiation codon; and (2) inserting 1bp between 3991bp and 3990bp at the upstream of the initiation codon. The application comprises the following steps: i) increasing the grain density, the spikelet number per ear, the secondary branch number or the yield of the oryza plants; ii) cultivating a transgenic Oryza plant; the FZP gene promoter mutant has the advantages that the FZP gene promoter mutant can be used for regulating and controlling the stem differentiation process of rice, promoting the increase of the grain density of the rice and increasing the yield of the rice, and the FZP gene promoter mutant has an important application value.

The invention provides application of an OsZIP13 gene. The OsZIP13 gene is used for regulating and controlling saline-alkali stress tolerance of rice plants. The OsZIP13 gene in the rice plant is over-expressed and is used for enhancing the saline-alkali stress tolerance of the rice plant; or / and the OsZIP13 gene in the rice plant is subjected to gene editing and is used for reducing the saline-alkali stress tolerance of the rice plant; the nucleotide sequence of the coding region of the OsZIP13 gene is as shown in SEQ ID No: 1; the amino acid sequence of the coding region of the OsZIP13 gene is as shown in SEQ ID No: 2. The gene is excessively expressed in rice, the tolerance of rice to saline-alkali stress in the germination stage and the seedling stage can be enhanced, a mutant strain is more sensitive to saline-alkali stress compared with wild rice, and the discovery of the gene provides an important gene resource and a theoretical basis for cultivation of saline-alkali tolerant plants; wide application prospects are realized.

The invention discloses a construction method of an unmanned aerial vehicle multi-spectral remote sensing basic model based on agricultural application, and relates to the technical field of intelligent agricultural equipment, and the method comprises the following steps: S1, collecting a multi-band remote sensing image of a rice region through a multi-spectral camera carried by an unmanned aerial vehicle; s2, calculating an anti-flooding vegetation index, and generating a vegetation coverage rate initial value according to the anti-flooding vegetation index; s3, extracting a rice plant area image, and inputting the rice plant area image into the trained rice state recognition model; s4, judging whether the flooding stress probability is greater than a flooding threshold value or not; and S5, determining the rice growth potential grade based on the vegetation coverage rate initial value or the corrected vegetation coverage rate. According to the method, through the anti-flooding vegetation index and the self-adaptive calculation algorithm in the turbid water body, the strong absorption interference of the water body in the near-infrared band is effectively stripped, so that the initial value of the vegetation coverage rate can truly reflect the survival state of underwater vegetation, and the situation that part of flooded healthy rice is misjudged as poor growth or death is fundamentally avoided.

We offer a furikake (rice seasoning) product that allows you to enjoy the unique flavor of the leaves and stems of the wild rice plant. [Solution] The seasoning product 10 containing Makomo charcoal powder comprises a powdered seasoning 200 and a container body 100 for holding the seasoning. The container body has a storage section 140 for holding the seasoning, a first zipper member 162, and a second zipper member 164, and the storage section can be repeatedly sealed by interlocking the first zipper member and the second zipper member. The seasoning is a powder mixture of Makomo leaf powder, which is made by crushing dried Makomo leaves, Makomo charcoal powder, which is made by crushing carbonized Makomo stems, kelp powder, which is made by crushing dried kelp, salt koji powder, which is made by crushing dried salt koji, and salt powder.

The utility model relates to the technical field of rice planting, and discloses a rice seedling loading and transporting device for rice planting, which comprises a vehicle body and a covering assembly, and the top of the vehicle body is fixedly connected with a placing frame. The covering assembly comprises a lead screw rotationally connected to one side of the top of the vehicle body through a first connecting frame, the outer portion of the lead screw is in threaded connection with a threaded block, the other side of the top of the vehicle body is fixedly connected with a guide rod through a second connecting frame, and the outer portion of the guide rod is slidably connected with a guide block. A connecting seat is fixedly connected between the guide block and the threaded block, a roller is rotationally connected to the connecting seat through a rotating shaft, a thin film is fixedly connected to the fixing plate, the tail end of the thin film is wound around the outer portion of the roller, and a gear is coaxially and fixedly connected to one end of the rotating shaft; the two second connecting frames are fixedly connected with the same rack, and the rack is connected with the gear in an engaged mode. The problem that the survival rate of rice seedlings is affected due to water evaporation during transportation is effectively solved.

The invention discloses a gene for promoting low-nitrogen efficient utilization of rice and enlarging grains and application of the gene. The gene is derived from rice and is named as OsUGT, and the nucleotide sequence of the gene is shown as SEQ ID NO.1; the amino acid sequence of the protein coded by the gene is as shown in SEQ ID NO. 2. The invention provides a construction method of an OsUGT gene overexpressed transgenic rice strain, which comprises the following steps: constructing pRHVc-OsUGT recombinant plasmids, transforming the pRHVc-OsUGT recombinant plasmids into calluses through an agrobacterium tumefaciens-mediated method, and screening resistant calluses and regenerating plants to obtain the OsUGT gene overexpressed rice. The OsUGT gene is overexpressed in a rice plant by adopting a pRHVc vector through a transgenic technology, so that the nitrogen utilization efficiency of the plant in a medium-low nitrogen stress environment can be remarkably improved, grain enlargement and grain weight increase can be effectively promoted, stable improvement of the crop yield under a low-nitrogen condition is realized, and the technical effect is clear and efficient.