277 results about "Rice plant" patented technology

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an SCRE10 gene for improving rice disease resistance and application of the SCRE10 gene, the base sequence of the SCRE10 gene is shown as SEQ ID NO.1, and the amino acid sequence of the SCRE10 gene is shown as SEQ ID NO.2. Through construction of a dexamethasone induced expression SCRE10 transgenic rice plant, it is found that the transgenic rice can significantly induce PR gene expression and active oxygen outbreak, and the resistance of rice to false smut and bacterial leaf blight can be improved. The invention proves that heterologous inducible expression of the Ustilaginoidea virens SCRE10 gene has the function of positively regulating the disease resistance of the rice, and the SCRE10 gene can be used for improving the disease resistance of the rice, which is of great significance to the creation of disease-resistant germplasm of the rice.

The invention provides a rice variable rate fertilization method and device, electronic equipment and a storage medium, and relates to the technical field of agricultural intelligent information process.The method comprises the steps that non-rice plant interferents in a multispectral remote sensing image are removed, and a rice plant canopy mask is generated; based on the rice plant canopy mask, multi-dimensional features of the rice plant canopy are extracted and fused, and a fused growth feature vector is obtained; grading the rice growth vigor according to the fused growth vigor feature vector through a growth vigor grade prediction model to obtain a predicted growth vigor grade; performing standardization processing on the predicted growth vigor level to obtain a standardized growth vigor level; determining a nitrogen fertilizer adjustment factor based on the standardized growth vigor grade; and according to the nitrogen fertilizer adjustment factor, adjusting a standard nitrogen application amount to obtain a recommended nitrogen application amount. According to the invention, accurate recommendation of the nitrogen application amount in the rice planting process can be realized, so that accurate fertilization is realized.

The present invention relates to methods for controlling or preventing infestation of a rice plant by the phytopathogenic microorganism Gibberella fujikuroi, comprising applying to a crop of rice plants, the locus thereof, or propagation material thereof, a compound according to formula (I) wherein R1, R2, R3, R4, R5, Y, A, B are as defined herein.

The invention relates to the technical field of rice phenotype extraction, and discloses a rice fine phenotype extraction method based on three-dimensional vision, a terminal and a storage medium. The method comprises the following steps: acquiring a multi-view image of a rice plant by using a depth camera, and performing camera attitude estimation on the multi-view enhanced image to obtain camera internal and external parameters corresponding to each view; constructing a neural radiation field network, training the neural radiation field network by taking the enhanced image, the contour mask and internal and external parameters of the camera as input, and learning color and density distribution of a scene by utilizing a volume rendering technology; extracting surface points based on a mixed threshold strategy by using the trained neural radiation field network, and generating a rice three-dimensional point cloud; inputting the rice three-dimensional point cloud into a point cloud instance segmentation network, and outputting an instance mask containing each organ of the rice and a segmentation result of a three-dimensional bounding box; and counting phenotypic parameters of the rice according to a segmentation result. According to the method, the fine instance segmentation of the high-density point cloud is realized while the reconstruction precision is ensured, and the phenotypic analysis of the spike fraction is supported.

The invention discloses a Chimonobambusa chensinensis CuCCoAOMT1 gene as well as a protein and application thereof, belongs to the technical field of plant genetic engineering, and particularly relates to a Chimonobambusa chensinensis caffeoyl-coenzyme A-O-methyltransferase CuCCoAOMT1 gene, and a nucleotide sequence of the CuCCoAOMT1 gene is shown as SEQ ID NO.1. The invention further discloses a preparation method of the CuCCoAOMT1 gene. The invention further discloses application of the CuCCoAOMT1 gene in regulation and control of the drought resistance of gramineous plants. By overexpressing the caffeoyl-coenzyme A-O-methyltransferase CuCCoAOMT1 gene in the rice, the lignin content in the rice can be effectively increased, the thickening of rice cell walls is promoted, the drought resistance of rice plants is improved, and a new gene resource is provided for plant material property improvement gene engineering.

The invention discloses a rice blast prevention and control and fertilizer efficiency improvement dual-objective optimization method, and relates to the technical field of agricultural informatics, and the method comprises the following steps: S1, data collection: obtaining rice field environment parameters, rice plant physiological parameters and pathogenic bacteria infection pressure indexes in real time; s2, constructing a double-objective collaborative optimization model: based on the data in the step S1, establishing a dynamic decision-making model which takes the maximization of the fertilizer efficiency utilization rate and the minimization of the rice blast occurrence risk as a combined objective. According to the method, a dynamic decision-making model taking maximization of the nitrogen fertilizer utilization rate and minimization of the rice blast severity as a combined target is constructed, the nitrogen fertilizer application amount, the bactericide type and the time sequence are subjected to collaborative optimization, a weight coefficient is introduced into the model, the target priority is dynamically adjusted according to the growth period, and the nitrogen fertilizer application amount is restrained in an agronomic safety interval; diseases caused by excessive application of nitrogen are avoided, positive correlation between the nitrogen fertilizer and the diseases is reduced from the source, pesticide dependence is reduced, and double improvement of the control effect and the fertilizer effect is achieved.

The application provides a method for increasing the length of a main panicle of rice, the number of secondary branches of the main panicle, the number of grains on the main panicle of rice and / or improving the yield of rice, which comprises overexpressing a cystathionine beta-lyase gene OsCBL in rice by using a recombinant vector containing a CDS coding sequence of the cystathionine beta-lyase gene OsCBL as shown in SEQ ID NO: 2 to obtain a transgenic plant. The transgenic plant obtained by the method has an increased number of grains per panicle and an increased yield per plant compared with normal plants. The method can be used for treating rice plants to improve the yield of rice and provides a new gene resource and a new idea for breeding rice with increased yield.

The application discloses a gene marker for identifying the sensitivity of rice flowering to sunlight, which comprises SNP site sequences selected from the group consisting of SEQ ID NOs:1-3 of an Hd1 gene and SEQ ID NOs:4-5 of a Ghd8 gene. The gene marker can be used for identifying the sensitivity of rice flowering to sunlight, and effectively guides the rapid breeding of rice varieties.

A rice direct seeding seed coating method with iron powder as main raw material, comprising the following steps: 1) weighing rice seeds and pouring into a coating machine, carrying out spiral mixing, spraying water on the surface of the rice seeds in the coating machine to make the surface of the rice seeds evenly wet; 2) adding raw materials to the rice seeds in the coating machine for at least two times, adding sodium carboxymethyl cellulose, calcium sulfate powder, iron powder and calcium peroxide powder in sequence in steps each time, and finally adding a fungicide; the raw material dosages are based on the weight of the rice seeds: sodium carboxymethyl cellulose 1.5-3%, calcium sulfate CaSO4·2H2O 2-6%, calcium peroxide CaO2 4-8%, iron powder 35-45%, and the fungicide 0.1-0.4%, and the water addition amount is not more than 30%; 3) spreading the coated rice seeds into a container and drying under ventilation. The present application adopts iron powder to coat the rice seeds for direct seeding, without seed disinfection, with deeper seed fall depth, improved seed germination rate, enhanced rice plant lodging resistance, and high-efficiency weed control by direct seeding in water.

Provided are a rice mutant gene and the use thereof in regulating the plant height of rice. The rice mutant gene at least has an allele mutation, and the allele mutation is the mutation into Asn from Asp at the 377th site in an amino acid sequence shown as SEQ ID No. 26. The mutant gene can effectively regulate and control the rice plant height, and has good application potential in plant architecture breeding of rice varieties.

The invention relates to the technical field of plant breeding, in particular to a mutant of an FZP gene promoter and application of the mutant. Compared with a wild type rice FZP gene promoter, the mutant has any one of the following mutations: (1) 5bp is deleted from 3995bp to 3989bp at the upstream of an initiation codon; and (2) inserting 1bp between 3991bp and 3990bp at the upstream of the initiation codon. The application comprises the following steps: i) increasing the grain density, the spikelet number per ear, the secondary branch number or the yield of the oryza plants; ii) cultivating a transgenic Oryza plant; the FZP gene promoter mutant has the advantages that the FZP gene promoter mutant can be used for regulating and controlling the stem differentiation process of rice, promoting the increase of the grain density of the rice and increasing the yield of the rice, and the FZP gene promoter mutant has an important application value.

The invention provides application of an OsZIP13 gene. The OsZIP13 gene is used for regulating and controlling saline-alkali stress tolerance of rice plants. The OsZIP13 gene in the rice plant is over-expressed and is used for enhancing the saline-alkali stress tolerance of the rice plant; or / and the OsZIP13 gene in the rice plant is subjected to gene editing and is used for reducing the saline-alkali stress tolerance of the rice plant; the nucleotide sequence of the coding region of the OsZIP13 gene is as shown in SEQ ID No: 1; the amino acid sequence of the coding region of the OsZIP13 gene is as shown in SEQ ID No: 2. The gene is excessively expressed in rice, the tolerance of rice to saline-alkali stress in the germination stage and the seedling stage can be enhanced, a mutant strain is more sensitive to saline-alkali stress compared with wild rice, and the discovery of the gene provides an important gene resource and a theoretical basis for cultivation of saline-alkali tolerant plants; wide application prospects are realized.

The invention discloses a construction method of an unmanned aerial vehicle multi-spectral remote sensing basic model based on agricultural application, and relates to the technical field of intelligent agricultural equipment, and the method comprises the following steps: S1, collecting a multi-band remote sensing image of a rice region through a multi-spectral camera carried by an unmanned aerial vehicle; s2, calculating an anti-flooding vegetation index, and generating a vegetation coverage rate initial value according to the anti-flooding vegetation index; s3, extracting a rice plant area image, and inputting the rice plant area image into the trained rice state recognition model; s4, judging whether the flooding stress probability is greater than a flooding threshold value or not; and S5, determining the rice growth potential grade based on the vegetation coverage rate initial value or the corrected vegetation coverage rate. According to the method, through the anti-flooding vegetation index and the self-adaptive calculation algorithm in the turbid water body, the strong absorption interference of the water body in the near-infrared band is effectively stripped, so that the initial value of the vegetation coverage rate can truly reflect the survival state of underwater vegetation, and the situation that part of flooded healthy rice is misjudged as poor growth or death is fundamentally avoided.

We offer a furikake (rice seasoning) product that allows you to enjoy the unique flavor of the leaves and stems of the wild rice plant. [Solution] The seasoning product 10 containing Makomo charcoal powder comprises a powdered seasoning 200 and a container body 100 for holding the seasoning. The container body has a storage section 140 for holding the seasoning, a first zipper member 162, and a second zipper member 164, and the storage section can be repeatedly sealed by interlocking the first zipper member and the second zipper member. The seasoning is a powder mixture of Makomo leaf powder, which is made by crushing dried Makomo leaves, Makomo charcoal powder, which is made by crushing carbonized Makomo stems, kelp powder, which is made by crushing dried kelp, salt koji powder, which is made by crushing dried salt koji, and salt powder.

The utility model relates to the technical field of rice planting, and discloses a rice seedling loading and transporting device for rice planting, which comprises a vehicle body and a covering assembly, and the top of the vehicle body is fixedly connected with a placing frame. The covering assembly comprises a lead screw rotationally connected to one side of the top of the vehicle body through a first connecting frame, the outer portion of the lead screw is in threaded connection with a threaded block, the other side of the top of the vehicle body is fixedly connected with a guide rod through a second connecting frame, and the outer portion of the guide rod is slidably connected with a guide block. A connecting seat is fixedly connected between the guide block and the threaded block, a roller is rotationally connected to the connecting seat through a rotating shaft, a thin film is fixedly connected to the fixing plate, the tail end of the thin film is wound around the outer portion of the roller, and a gear is coaxially and fixedly connected to one end of the rotating shaft; the two second connecting frames are fixedly connected with the same rack, and the rack is connected with the gear in an engaged mode. The problem that the survival rate of rice seedlings is affected due to water evaporation during transportation is effectively solved.

The invention discloses a gene for promoting low-nitrogen efficient utilization of rice and enlarging grains and application of the gene. The gene is derived from rice and is named as OsUGT, and the nucleotide sequence of the gene is shown as SEQ ID NO.1; the amino acid sequence of the protein coded by the gene is as shown in SEQ ID NO. 2. The invention provides a construction method of an OsUGT gene overexpressed transgenic rice strain, which comprises the following steps: constructing pRHVc-OsUGT recombinant plasmids, transforming the pRHVc-OsUGT recombinant plasmids into calluses through an agrobacterium tumefaciens-mediated method, and screening resistant calluses and regenerating plants to obtain the OsUGT gene overexpressed rice. The OsUGT gene is overexpressed in a rice plant by adopting a pRHVc vector through a transgenic technology, so that the nitrogen utilization efficiency of the plant in a medium-low nitrogen stress environment can be remarkably improved, grain enlargement and grain weight increase can be effectively promoted, stable improvement of the crop yield under a low-nitrogen condition is realized, and the technical effect is clear and efficient.

The invention discloses a rice fork head related structural domain protein coding gene OsFHA11 and application thereof, and belongs to the technical field of plant genetic engineering. According to the invention, the OsFHA11 gene is connected with an overexpression vector through a genetic engineering means and then is transformed into rice, so that the expression of the gene in the transformed rice plant is improved, and the resistance of the rice plant to sheath blight can be improved. Through cloning of the OsFHA11 gene and analysis of the sheath blight resistance function of the OsFHA11 gene, analysis of a molecular mechanism of rice sheath blight resistance is facilitated. In practice, a sheath blight resistant rice material can be cultivated by using the gene through a transgenic means, and a sheath blight resistant rice variety with stable characters is further obtained through interspecies hybridization, so that the occurrence of sheath blight is effectively controlled, and the rice yield is increased.

The application relates to the computer field and provides a rice potential yield estimation method and device. The method comprises the following steps: identifying a rice planting mode of a to-be-estimated area according to synthetic aperture radar data; dividing different rice planting areas according to different rice planting modes in the to-be-estimated area; performing rice plant density inversion on different rice planting areas to obtain rice plant densities corresponding to the different rice planting areas; and estimating the rice potential yield in the corresponding rice planting area according to the different rice plant densities. The rice potential yield estimation method and device provided in the application can obtain more accurate rice plant densities in the corresponding rice planting area according to continuous large-range data in the to-be-estimated area, so that the rice potential yield in the corresponding rice planting area can be accurately estimated according to the rice plant density.

The invention discloses application of an OsRPP13 gene in regulation and control of heat resistance and photosynthetic performance of rice. According to the invention, a CRISPR-Cas9 carrier is constructed, and rice OsRPP13 gene knockout mutant strains (osrpp13-2, osrpp13-18 and osrpp13-20) are obtained by using an agrobacterium tumefaciens-mediated transformation method. The inventor discloses the function of the OsRPP13 gene in the aspect of regulating and controlling the temperature tolerance and the photosynthetic performance of a rice plant by improving a culture temperature simulation experiment. Through the research, a gene resource is provided for high-temperature-resistant and high-photosynthetic-efficiency molecular breeding of rice. The rice can survive and grow in a temperature range exceeding the original adaptive temperature range, the photosynthetic efficiency is improved, and a gene resource is provided for high-photosynthetic-efficiency molecular breeding and genetic improvement of the rice.

The present application relates to the technical field of rice molecular breeding, and particularly relates to a method for cultivating high-yield rice by overexpressing an OsalphaCA11 gene and application thereof, the method for cultivating high-yield rice comprising increasing the expression amount of the OsalphaCA11 gene, and then increasing the content of OsalphaCA11 protein in rice plants; the amino acid sequence of the OsalphaCA11 gene is shown as SEQ ID NO. 1, and the nucleotide sequence of the OsalphaCA11 gene is shown as SEQ ID NO. 2. After increasing the expression amount of the OsalphaCA11 gene, the grain number of the top ear and the total grain number per plant of the rice are both increased, and the grain type is increased, thereby increasing the yield per plant of the rice; the overexpression of the OsalphaCA11 gene can be applied to the improvement of agronomic traits related to increasing the grain number of the top ear, the grain number per plant and increasing the grain type, thereby creating conditions for increasing the yield of rice and cultivating new high-yield rice strains.

The invention discloses an AP2 gene or an encoding protein thereof and application of the AP2 gene or the encoding protein thereof in increasing plant yield or promoting plant growth. The ZmAP2 gene is identified from corn and is overexpressed in rice, and compared with a control CK, the number of grains per ear of an overexpressed plant is remarkably increased, the grain length is remarkably increased, the thousand grain weight is remarkably increased, the biomass is remarkably increased, and the plant height is remarkably increased; the homologous rice OsAP2 gene is further subjected to overexpression in rice, and compared with a control CK, the overexpressed rice plant grain number per ear is remarkably increased, grains are remarkably lengthened, the thousand grain weight is remarkably increased, and the yield of a single plant is remarkably increased. The invention has application potential in the aspects of promoting crop growth, improving crop yield, cultivating high-yield crop varieties and the like.

The utility model belongs to rice planting technical field especially, and a kind of germination equipment for rice seed production, to the existing problem of low efficiency, embryo is easily hurt, frequent opening box destroys temperature and humidity stability, and the problem of inconveniently, difficult operation of fixed placement tray, the following scheme is presented, including cultivation box, the opening of cultivation box is hinged with sealing door, its inside is fixed with multiple groups of support components, slidingly set with placement tray on each support component, in the utility model, rotation pipe automatic seed turning is realized by turning component, avoid artificial damage embryo and improve efficiency, and sealing door does not need to be opened frequently;When driving component drives moving pipe, spraying and turning are synchronous, cooperate with monitoring component to adjust temperature and humidity, reduce the environmental fluctuation in box;The sliding connection structure of support component makes placement tray convenient to pull out or push in, limit pin locks position, solve the problem of inconveniently taking and placing.

This invention relates to a gene qAC that regulates amylose content in rice and a method for improving amylose content using the qAC gene. The qAC gene includes qAC6.1, qAC6.2, and qAC9.1 genes. The nucleotide sequence of the coding region of the qAC6.1 gene is shown in SEQ ID NO.1, the nucleotide sequence of the coding region of the qAC6.2 gene is shown in SEQ ID NO.3, and the nucleotide sequence of the coding region of the qAC9.1 gene is shown in SEQ ID NO.5. The amino acid sequence of the coding region of the qAC6.1 gene is shown in SEQ ID NO.2, the amino acid sequence of the coding region of the qAC6.2 gene is shown in SEQ ID NO.4, and the amino acid sequence of the coding region of the qAC9.1 gene is shown in SEQ ID NO.6. Through this invention, the rice qAC gene is highly expressed in seeds, specifically improving the amylose content of rice grains without significantly affecting other major agronomic traits of the rice plant. Knocking out the qAC gene in rice using genetic engineering technology can significantly reduce the amylose content of rice and improve the cooking and eating quality of rice. Therefore, the qAC gene has a promising application prospect in the breeding of high-quality rice.

This invention discloses the application of the OsSMP3 gene in the control of rice brown planthopper, specifically disclosing that the OsSMP3 gene (LOC_Os11g05170) negatively regulates rice resistance to brown planthopper. By targeting this gene using CRISPR / Cas9 gene editing technology, a loss-of-function mutant was obtained. Compared to the wild type, the knockout mutant showed significantly enhanced resistance to brown planthopper, specifically manifested in increased rice plant survival rate. This invention provides new gene resources and targets for molecular breeding of rice resistant to brown planthopper.

To provide a brown rice quality improving agent and a method for improving quality of brown rice, with which brown rice with high quality can be provided. The brown rice quality improving agent of the present invention contains zerumbone as an active ingredient. The method for improving quality of brown rice of the present invention has a step of applying zerumbone to rice plants in the cultivation period.

The invention relates to a rice gene OsMYB63L and application of the rice gene OsMYB63L in regulation and control of spike branching and yield, and belongs to the technical field of plant gene breeding, the nucleotide sequence of the gene OsMYB63L is as shown in SEQ ID NO.1, the amino acid sequence of protein coded by the gene is as shown in SEQ ID NO.2, overexpression of the gene OsMYB63L promotes development of the spike branching of rice, then the yield of rice is increased, a transgenic technology is utilized, and the yield of the rice is increased. Overexpression is carried out on the rice gene OsMYB63L, and a rice plant with increased ear branch number and increased yield can be obtained. The invention discovers and verifies that the rice gene OsMYB63L has the function of regulating and controlling the ear branching number and the yield of rice. And a new gene resource and a technical path are provided for molecular breeding of high-yield rice.

This invention discloses a rice OsRbohB protein, its encoding gene, and its applications. The amino acid sequence of the OsRbohB protein is shown in SEQ ID NO.2, and the nucleotide sequence of its encoding gene is shown in SEQ ID NO.1. This invention provides a rice transformation overexpression vector that overexpresses the OsRbohB gene using the ZmUbi promoter. The plant height, number of grains per panicle, and grain length of the overexpressed plants were significantly increased. This invention is the first to demonstrate that rice OsRbohB is a functional gene regulating rice plant height, number of grains per panicle, and grain length. The cloning and biological function verification of this gene have important reference value for the study of the molecular mechanisms regulating important agronomic traits in rice. Since the above traits are important traits related to rice plant type and yield, the functional analysis of OsRbohB in this invention provides a new candidate gene for rice design breeding and has great potential application value.

The embodiment of the invention provides a rice plant counting method based on a low-altitude unmanned aerial vehicle image, a group perception adaptive mechanism and inverted density scale fusion loss. The method comprises the steps of S1, data preprocessing; s2, constructing an encoder-decoder network model, in which an encoder adopts a pre-trained VGG model as a backbone network, and a decoder performs feature enhancement and weight importance screening on the input feature map by using a group perception adaptive mechanism; s3, training the encoder-decoder network model to obtain a counting model, and fusing a loss function by adopting an inverted density scale in the training process; and S4, carrying out forward propagation on the input rice image by using the counting model, outputting a prediction density map, and summing the prediction density map to obtain a rice plant counting result. According to the method, the capability of extracting the key features of the plants is enhanced, multi-scale self-adaptive supervision is realized, and the counting precision and robustness in complex field environments such as dense and shielded environments are remarkably improved.