966 results about "Rice plant" patented technology

The invention discloses a method for planting se-enriched rice. The method comprises the following steps of land selection and preparation; seeding; seedling culturing; transplanting; field management: intertillage, weeding, topdressing and insect pest prevention; and harvesting. The selenium content of rice planted by using the method is high, and the yield of the rice is also high. Testing confirms that the yield of the se-enriched rice is 620-650kg per acre, and the selenium content of the rice ranges from 0.1 mg/kg to 0.3mg/kg.

The rice plant cultivation and strengthening agent and using method thereof relate to plant cultivation and strengthening agent and using method thereof, which solves the problems of less nutrition content, larger dosage, high production and transport cost, high labor intensity and complexity in fertilization, labor and time consumption in prior rice plant cultivation and strengthening agent. The rice plant cultivation and strengthening agent comprises nutrition elements, flavohumic acid, acid regulating agent, sequestering agent, water conservation agent, disinfectant, plant growth regulator, phlogiston, mould inhibitor, and additive. The using method is that: 1. the rice plant cultivation and strengthening agent is added with water in a proportion of 1 to 1000 times; 2. the solution is applied in the land with a proportion of 500g of the agent to 50m2 of the land; 3. the rice is planted when the agent is fully permeated in the soil. The rice plant cultivation and strengthening agent of the invention has high content of nutrition and the dosage of 10g in 1m2 land, thereby reducing production and transport cost and labor intensity in fertilization. The using of the rice plant cultivation and strengthening agent combines the fertilization, pH regulation, disinfection and first watering together, which is of time and labor saving.

The invention provides an ecological water conservancy restoration system and method for heavy metal polluted farmland soil. The ecological water conservancy restoration method comprises elution and purification of soil heavy metals in a planting intermission period and water and fertilizer combined prevention and control of soil residual heavy metals in a planting period; in a rice planting intermission period, circular treatment of environmental-protection elution, field water recession and forced drainage and receded water ecological purification is carried out on heavy metal polluted soil sequentially, so that overproof heavy metals in rice soil can be gradually transferred into a water body to be treated, and thus the rice field is cleaned; in a rice planting period, field water and fertilizer combined prevention and control is carried out on the residual heavy metals after the rice soil is eluted, so as to prevent and control the heavy metals from entering the rice; a series of treatment measures to the soil heavy metals in the rice planting intermission period and the planting period can ensure the treatment on symptoms and root causes of the heavy metal polluted rice soil and further can ensure the safe planting of rice.

The invention discloses an artificial fixed-point rice dense and erect panicle (DEP1) gene mutant body and application thereof. The rice DEP1 gene mutant body as well as a corresponding allele is obtained by modifying a 5th exon of a DEP1 gene by adopting a CRISPR/Cas9 gene targeting modification technology, so that a base of the 5th exon is replaced, lost and inserted. The artificial fixed-point rice DEP1 gene mutant body and the application thereof disclosed by the invention have the benefits that the artificial mutation is performed on the 5th exon of the rice DEP1 gene by adopting the CRISPR/Cas9, and the mutant body capable of obviously improving the rice yield and the allele are screened; the DEP1 gene mutant body disclosed by the invention as well as the corresponding allele can improve the yield of rice plants by 13-51 percent and is even superior to a natural mutation type.

The present invention is in the field of plant biochemistry and genetics. More specifically the invention relates to nucleic acid molecules from plant cells, in particular, genomic DNA sequences from rice plants and nucleic acid molecules that contain markers, in particular, single nucleotide polymorphism (SNP) and repetitive element markers. In addition, the present invention provides nucleic acid molecules having regulatory elements or encoding proteins or fragments thereof. The invention also relates to proteins and fragments of proteins so encoded and antibodies capable of binding the proteins. The invention also relates to methods of using the nucleic acid molecules, markers, repetitive elements and fragments of repetitive elements, regulatory elements, proteins and fragments of proteins, and antibodies, for example for genome mapping, gene identification and analysis, plant breeding, preparation of constructs for use in plant gene expression, and transgenic plants.

The invention discloses an ecological fish farming method in a rice field, which comprises the following steps of rice field preparation, rice field transformation, fish farming facility preparation, rice seedling transplantation, biosorption, rice field disinfection, irrigation, fertilization, stocking fish seed selection, stocking fish fry disinfection, stocking and feeding management, wherein annular field fish ditches dug through the rice field transformation are V-shaped ditches arranged on the inner sides of ridges around the rice field; the biosorption utilizes a biosorption agent to adsorb heavy metal in rice field soil and irrigation water; and stocking fish seeds are selected from eight-beard catfishes. The invention further discloses rice produced by rice plants planted in the rice field using the ecological fish farming method. According to the method and the rice, an effective usable area of the rice field is increased significantly; pollution of the heavy metal such as lead and cadmium is reduced; integral benefits of fish farming in the rice field are improved; the ecological rice contains rich protein, more microelements such as iron, magnesium and calcium, and vitamin C, has no chemical pesticide residual, and is green and environment-friendly; and the content of the heavy metal such as lead and cadmium is very low.

The invention belongs to the field of agricultural robots, in particular to a small-sized inter-row weeding robot for paddy fields, which comprises a chassis, a drive system, a control system, a sensing system, a weeding device and a power source. A drive motor drives a spindle to rotate so as to drive paddle wheels, and the paddle wheels rotate at different speeds to turn the robot. A direct current motor drives a weeding disc blade to rotate to cut off inter-row weeds on a paddy field. Silt generated in travelling of the paddle wheels covers the weeds among plants to inhibit the inter-plant weeds. After a limit switch contacts with rice plants, a single chip microcomputer controls the left motor and the right motor to rotate at different speeds so as to lead the weeding robot to turn and avoid the plants. When a pressure sensor touches a ridge, the single chip microcomputer controls the drive motor to rotate reversely and the weeding robot continues operating in the opposite direction. The sensing system is capable of measuring information such as paddy field water temperature, plant amount and the like. The small-sized inter-row weeding robot for paddy fields is operated in a mechanical weeding mode, so that usage of pesticides and weeding labor are reduced, environment pollution by the pesticides is lessened, and cost is saved. The small-sized inter-row weeding robot for paddy fields is small in size and light in weight.

The invention discloses a CRISPR/Cpf1 system-mediated homologous recombination method using an RNA transcript as a repair template. The method adopts rice ALS genes as a research object, and constructs a homologous recombination vector. RCR1-RCR2-RDR fragments are transcribed in vitro, a RNP method is used, the RNA transcript is used as the repair template, and homologous recombination repair of target genes is achieved in rice callus. The vector is introduced into the rice callus by a gene gun method, and rice plants with ALS gene fixed-point modification are obtained. The results show that RNA used as the repair template can successfully mediate homologous recombination of the target genes, and the method provides a novel idea for crop breeding, and has strong application potential in agricultural breeding.

A rice and lobster co-culture method includes a rice planting part and a lobster breeding part. The lobster breeding part includes the steps of (1) rice field modification; (2) juvenile shrimp culture; (3) adult shrimp breeding; (4) parent shrimp putting; (5) adult shrimp fishing, supplementary juvenile shrimp putting and parent shrimp retention. The rice planting part includes the steps of (1) rice cultivation; (2) rice field management; (3) field drying; (4) rice pest and disease control; (5) draining and harvesting. Compared with an ordinary rice and lobster co-culture mode, the morbidity frequency of lobsters can be reduced to 45 days or so from 7-10 days, the disease control cost is reduced by 150 yuan/mu or so, and the survival rate can be increased to 80% or above from 30-40%.

The invention provides a method for preventing and controlling rice grubs by breeding soft-shelled turtle in a rice field, comprising the following steps of: (1) breeding strong rice seedlings, and transplanting the rice seedlings to large field; (2) digging deep ditches at the periphery and the middle part of the rice field; (3) setting habitats for the soft-shelled turtle at the middle part of the rice field and at the two sides of the rice field; (4) mounting escaping (stealing)-preventing facilities; (5) selecting young soft-shelled turtles; and (6) determining breeding time and density of soft-shelled turtle and disinfecting the young soft-shelled turtles, wherein Chinese soft-shelled turtle can be bred two days after rice is transplanted, and 30-50 Chinese soft-shelled turtles with the weight of 250-300g can be bred every 667 square meters. By adopting the method provided by the invention, quantity of spiders in the rice field can be improved, the quantity of rice plant hoppers can be reduced, ratio of the spiders to the rice plant hoppers is improved by 45.23%, damage of the rice plant hoppers is obviously reduced, control effect on cnaphalocrocis medinalis is 38.89%, and dead heart rate caused by damage of borers (chilo suppressalis and sesamia inferens) is reduced by 25% compared with that of the rice field in which the soft-shelled turtles are not bred.

The invention provides a rice ridge culture salt-avoiding water-saving planting method of severe soda saline-alkali soil. The characteristic that pH value and EC of the position of a ridge waist of a severe soda saline-alkali area are the lowest is utilized, rice seedlings are transplanted at the position of the ridge waist, damage of salt to rice root systems is avoided, and the environment suitable for normal growth of rice is created. The rice ridge culture salt-avoiding water-saving planting method of the severe soda saline-alkali soil solves the problems that the rice planting survival rate of the severe soda saline-alkali soil is low, growth of the rice is subjected to saline and alkaline limitation, and the like. The rice ridge culture salt-avoiding water-saving planting method of the severe soda saline-alkali soil is low in cost, reduces harrowing measures, improves soil, increases ground temperature, expands effective ploughing area, and is of great importance for increasing rice yield.

The invention discloses a DNA fragment separating colony and functional checking of rice tissue special expressing promoter PD54O in plant gene engineering technical domain, which is characterized by the following: the PD54O promoter expresses in rice leaf and leaf sheath and does not express in germ and endosperm; transferring genetic rice plant can resist the invasion of borer with the expression of PD54O regulate and control insect-resisting gene; it does not express Bt protein in seeds of food.

The invention belongs to the technical field of plant gene engineering, disclosing a separated and cloned major gene GS5 capable of controlling the width and the weight of a rice grain and the DNA sequence of the allelic gene of the major gene GS5. The DNA sequence is shown as SEQ ID NO.1 (Zhenshan 97B) and SEQ ID NO.3 (H94) and contains 10 exons. The amino acid sequence of the major gene and the amino acid sequence of the allelic gene are shown as SEQ ID NO.2 and SEQ ID NO.4. By using two large-grain rice varieties and two small-grain rice varieties for comparative sequencing, in an approximately 6.1kb range, 22 common base differences exist between the large-grain variety and the small-grain variety, wherein 18 mutations are in a promoter area, 4 mutations are in a code area and 5 amino acids are caused to be changed. By using a transgenic technology, GS5 transgenic rice plants are obtained and express that the width and the weight of the rice grain are obviously improved when being compared with the control width and the control weight of the rice grain. The character changes are quite coincident with the two genotype expressions of a Zhenshan 97 near-isogenic line and a GS5 near-isogenic line. The invention additionally discloses a method of near-isogenic line breeding, gene cloning and gene transfer and application thereof.

A rice cultivar designated Templeton is disclosed. The invention relates to the seeds of rice cultivar Templeton, to the plants of rice cultivar Templeton, to plant parts of rice cultivar Templeton, and to methods for producing a rice plant produced by crossing rice cultivar Templeton with itself or with another rice variety. The invention also relates to methods for producing a rice plant containing in its genetic material one or more transgenes and to the transgenic rice plants and plant parts produced by those methods. This invention also relates to rice cultivars, or breeding cultivars, and plant parts derived from rice cultivar Templeton, to methods for producing other rice cultivars, lines or plant parts derived from rice cultivar Templeton, and to the rice plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid rice seeds, plants, and plant parts produced by crossing rice cultivar Templeton with another rice cultivar.

Information of plant based on color of a surface of plant is acquired from image data obtained by imaging plant, allowing to acquire information of plant at low cost, compared to chlorophyll meter or spectroscopic analyzer. In crop production like rice plant, fertilization management including fertilizer application management like fertilizer amount determination, or other agricultural works, is supported through a smart phone or the like based on data to be observed, like converted leaf color value is calculated from image data obtained by crop imaging. Camera is connected to smart phone. Converted leaf color value can be obtained from image data obtained by imaging leaf of rice plant by camera. Converted leaf color value is transmitted to management server, for example, amount information of applied fertilizer is required in case where converted leaf color value is less than standard, can be obtained as management information for fertilizer application management.

The invention discloses application of a nitrate radical transporter gene OsNRT1.8 in rice breeding and belongs to the field of plant genetic engineering. The protein encoding amino acid sequence of the gene OsNRT1.8 is shown as SEQ ID NO.1, and a cDNA sequence is shown as SEQ ID NO.2. By establishing rice gene OsNRT1.8 overexpression plant and a gene OsNRT1.8 interference plant, it is found that normal rice tiller number and spike number of each plant can be increased by improving gene OsNRT1.8 expression. Therefore, the gene OsNRT1.8 can be used for increasing rice yield in rice breeding. The gene OsNRT1.8 has an important application value on the aspects of elaboration about nitrogen influence on plant growth and development and improvement of rice plant type.

The invention belongs to the technical field of non-point source pollution control, and discloses a method for controlling the non-point source pollution of paddy field, artificial paddy wetland system, and a paddy field wetland system for improving the P and N utilization rate of paddy field. The system structure uses the paddy field as the upper stream, and a paddy field wetland is constructed in the drainage downstream of the paddy field along the river. The surface of the paddy field wetland soil is 25-32cm lower than surface of the paddy field soil, and the area of the paddy field wetland is 3%-5% of the paddy field; the paddy filed wetland and paddy field are separated by the isolation ridge; paddy field ridges are around the paddy field, and paddy field wetland ridges are around the paddy field wetland; an irrigation ditch is arranged along one side of the paddy field and the paddy field wetland, and a drainage ditch is arranged on the other side. The method uses the paddy field to block, absorb and utilize the pollutant substances in the water, in order to reduce the pollutant substances in the output water; the paddy rice planted in the paddy field wetland uses the N, P and other elements in the drain away water from upstream paddy field as the source of nutrients, so as to increase the utilization rate of N and P in the paddy field.

The invention discloses a cultivation method of selenium-enriched high-yielding rice. The method is characterized by comprising the steps of selecting seeds, soaking the seeds, accelerating germination, sowing, performing field management, performing fertilization management and the like. The steps of soaking the seeds and accelerating germination can improve a germination rate and a survival rate after direct seeding, and reduce the use amount of the seeds; as for selenium-enriching, a rice base fertilizer is prepared by using an unique fermentation process; in the base fertilizer, inorganic selenium is combined with organic selenium in straws, so that selenium is slowly released at a low concentration; rice plants can be promoted to absorb selenium permanently and effectively, the inhibiting effect, on rice growth, caused by excessive concentration of selenium or applying selenium for several times also can be avoided, and therefore the yield of the rice is influenced.

The invention is directed to expression of non-plant proteins in rice plants. Expression is optimized by use of a non-rice promoter of a monocot protein gene and its corresponding signal peptide for expression of the non-plant protein in rice plant at high yields. The invention is useful for making human proteins, polypeptides and peptides in rice seeds. The expressed protein product can be isolated from the rice seed for administration to humans or other animals.

The invention discloses a method for planting high quality rice. The method comprises the steps of seed selection, seeding, rice transplanting, a panicle primordium differentiation stage, a heading period and a mature period. When rice ears fall and are golden, the rice can be reaped. Compared with the existing rice planting technology, the method has significant advantages.When rice planting is performed according to the technical scheme, the reaped rice produces full ears, the rice quality and quantity are improved greatly, the germination rate of rice seeds can be improved through the treatment of the rice seeds, fertilizer for rice is applied in the panicle primordium differentiation stage, and the fertilizer for the rice are added to fertilizer through conjugation reaction of bentonite, sodium carbonate and ferrous sulfate, so that the fertilizer efficiency is improved, the soil is improved, the absorption of the rice for the fertilizer is promoted, the rice can enter into the vegetative growth stage and reproductive growth stage side by side, and the vegetative reproduction of the rice is guaranteed to lay a solid foundation for head sprouting. According to the five-year continuous planting experiment statistics, the yield of the rice planting through the method is 18% to 20% higher than the yield of the rice planted by general planting methods.

The invention relates to a multi-functional glutinous rice plant liquid coating, and particularly relates to an environment-friendly, non-toxic and harmless biomass coating. The multi-functional glutinous rice plant liquid coating comprises, by mass, 10-15% of water, 2-5% of cellulose, 1-4% of a wetting agent, 1-4% of a defoaming agent, 10-30% of titanium dioxide, 15-40% of calcium carbonate, 15-35% of VAE, 2-25% of glutinous rice slurry, 5-10% of lime slurry supernatant, 0.5-1% of nano silver and 3-7% of plant extracts. With the above technical solution, the glutinous rice slurry, calcium carbonate and quicklime are mixedly used to produce an organic-inorganic composite material. The material is compact in structure, and has high mechanical strength, good toughness and a good sealing effect for matrix. Therefore, the obtained coating has excellent weather resistance and ageing resistance, and at the same time, by adding the plant extract, the coating is fresher and more natural. Besides, the obtained coating contains no harmful ingredient, is safe and environment-friendly and is simple in preparation.

Disclosed is a rice cropping method for improving the efficiency for solar energy utilization, the row direction of rice cropping is from southwest to northeast, and the angle is 20-25 degrees to the magnetic south westwards; the rice cropping row is a combined row of a ventilation and light penetrating super-large row and a rice planting small row, the row spacing of the ventilation and light penetrating super-large row is 60cm, and the row spacing of the rice planting small row is 20cm. Two rows of rice are planted on the rice planting small row with the plant spacing of 15cm, and the rice seedling transplanting adopts the zigzag transplanting method. The phenomenon of mutual overshadowing of rice plants in the effective photosynthesis time can be avoided by changing the tillage row direction and extending the row spacing, thus greatly increasing the whole plant illumination time and improving the intensity of illumination, as well as increasing the photosynthetic rate maximally. The application of the invention matched with the scientific farming, scientific management and duly early harvest technique can increase the percentage of earring-tillers by 13.3 percent, raise the soil temperature in the flowering stage by 0.4-2.1 DEG C and increase the soil temperature in the filling stage by 2.2 DEG C, the yield is increased by 838.5kg, up by 9.4 percent, and the efficiency for solar energy utilization of economic yield is increased by 0.16 percent.