450 results about "Rice plant" patented technology

The invention relates to the field of rice gene engineering, and particularly provides OsSULTR2; the invention also discloses application of the 2 protein and the coding gene thereof in regulating and controlling the salt tolerance of rice. The protein meets the following conditions: B1) a protein with an amino acid sequence of SEQ ID NO.1; and B2) a fusion protein with the same function obtained by connecting a tag to the N end and / or C end of B1). The method is used for detecting OsSULTR2; the salt stress phenotype identification in the seedling stage is carried out on the transgenic rice with the gene knockout 1, 2, and the result shows that when the gene segment is deleted, the salt stress tolerance of the rice is improved, and the function and the application way of the gene are proved. Therefore, the OsSULTR2 of the present invention; the 2 protein and the coding gene thereof can regulate and control the salt tolerance of rice, and have important significance for cultivating salt-tolerant transgenic rice.

The invention provides a Ustilaginoidea virens gene UvASE2 for regulating and controlling rice resistance and application of the Ustilaginoidea virens gene UvASE2, and relates to the technical field of plant genetic engineering. The nucleotide sequence of the Ustilaginoidea virens gene UvASE2 is as shown in SEQ ID NO: 1, and the amino acid sequence of the encoding protein of the Ustilaginoidea virens gene UvASE2 is as shown in SEQ ID NO: 2. By constructing a transgenic rice plant for heterologous expression of UvASE2, it is found that the transgenic rice can significantly improve the resistance to false smut and bacterial blight and significantly induce the outbreak of active oxygen. The heterologous expression UvASE2 gene of Ustilaginoidea virens has the function of positively regulating and controlling the disease resistance of rice, and the UvASE2 gene can be used for improving the disease resistance of rice and has important significance on creation of disease-resistant germplasm of rice.

The invention provides a rice yield prediction method and system based on multi-source time sequence remote sensing data, and relates to the field of agricultural informatization and remote sensing technology application, and the method comprises the steps: collecting multi-spectral data and laser radar data, and carrying out the precise geographic positioning of the data through a high-precision satellite positioning system, obtaining multi-source unmanned aerial vehicle remote sensing data; performing camera internal orientation, wave band registration, orthoimage splicing and embedding, radiation correction and remote sensing index calculation on the multispectral data to obtain processed multispectral data; and performing point cloud reconstruction and point cloud filtering on the laser radar data, and calculating the height of the rice plant to obtain processed laser radar data. According to the method, multi-source remote sensing data and spatio-temporal data are fused, and remote sensing characteristics of different phenological periods of rice are dynamically analyzed and filtered, so that the precision and reliability of rice field yield prediction are remarkably improved.

According to the rice field methane emission prediction method based on the CH4MN model, the influence of the rice field methane emission microorganism process and soil nitrogen is considered, the uncertainty of simulation is reduced, and rice field methane emission is accurately predicted. Comprising the following steps: S1, acquiring rice field agricultural production input, water management measures, meteorological data, observed rice field soil environment information, rice information and microorganism information as input data of a CH4MN model; s2, based on a CH4MN model algorithm, calculating a substrate source and a soil environment required by methane generation, and the influence of soil nitrogen on organic matter decomposition; s3, calculating the influence of soil oxidation reduction potential, microorganisms and rice plants on methane generation and oxidation based on a CH4MN model algorithm; s4, calculating a methane generation rate and an emission rate based on a CH4MN model algorithm by using the methane generation and oxidation influence factors calculated in the step S1-3; and S5, verifying the CH4MN model precision based on point observation information.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an SCRE10 gene for improving rice disease resistance and application of the SCRE10 gene, the base sequence of the SCRE10 gene is shown as SEQ ID NO.1, and the amino acid sequence of the SCRE10 gene is shown as SEQ ID NO.2. Through construction of a dexamethasone induced expression SCRE10 transgenic rice plant, it is found that the transgenic rice can significantly induce PR gene expression and active oxygen outbreak, and the resistance of rice to false smut and bacterial leaf blight can be improved. The invention proves that heterologous inducible expression of the Ustilaginoidea virens SCRE10 gene has the function of positively regulating the disease resistance of the rice, and the SCRE10 gene can be used for improving the disease resistance of the rice, which is of great significance to the creation of disease-resistant germplasm of the rice.

The invention discloses application of a rice gene OsPILS1 in regulation and control of rice yield, and relates to the technical field of rice transgenosis. According to the invention, after the gene OsPILS1 is over-expressed in rice 9311, the number of secondary branches and the number of grains per ear are obviously increased; an editing vector containing an OsPILS1 gene editing site is transferred into rice 9311 to carry out knockout editing on the gene, and phenotypic statistics show that the number of secondary branches of transgenic rice is obviously reduced, and the number of grains per ear and the yield of a single plant are also obviously reduced. The results show that the OsPILS1 gene can obviously increase the number of grains per panicle by regulating and controlling the development of secondary branches of rice, so that the rice yield is regulated and controlled. Therefore, the rice yield can be effectively regulated and controlled by regulating and controlling the expression level of the OsPILS1 gene in rice plants, and the OsPILS1 gene has a huge application prospect in scientific research of breeding new high-yield rice varieties by molecular breeding.

The invention relates to the technical field of gene engineering, and discloses an METS2 gene for regulating and controlling the grain shape of rice, a protein and application of the METS2 gene, and the nucleotide sequence of the METS2 gene is shown as SEQ ID NO.1. The invention further discloses application of the METS2 gene in regulation and control of the rice grain shape. According to the METS2 gene for regulating and controlling the rice grain shape, the protein and the application of the METS2 gene, the METS2 gene is overexpressed in a rice plant, short-grain-shaped rice can be obtained, the short-grain-shaped rice is not prone to breakage in the processing process, and the quality of a finished product is high; a basis is provided for further clarification of the molecular mechanism of the rice grain shape, and new gene resources and breeding resources are provided for rice breeding.

The invention provides a rice variable rate fertilization method and device, electronic equipment and a storage medium, and relates to the technical field of agricultural intelligent information process.The method comprises the steps that non-rice plant interferents in a multispectral remote sensing image are removed, and a rice plant canopy mask is generated; based on the rice plant canopy mask, multi-dimensional features of the rice plant canopy are extracted and fused, and a fused growth feature vector is obtained; grading the rice growth vigor according to the fused growth vigor feature vector through a growth vigor grade prediction model to obtain a predicted growth vigor grade; performing standardization processing on the predicted growth vigor level to obtain a standardized growth vigor level; determining a nitrogen fertilizer adjustment factor based on the standardized growth vigor grade; and according to the nitrogen fertilizer adjustment factor, adjusting a standard nitrogen application amount to obtain a recommended nitrogen application amount. According to the invention, accurate recommendation of the nitrogen application amount in the rice planting process can be realized, so that accurate fertilization is realized.

The invention relates to the technical field of gene engineering, and discloses a rice flood-resistant gene ST4 and application thereof. The nucleotide sequence of the rice flood-resistant gene ST4 is as shown in SEQ ID NO.1, and the coding sequence of the rice flood-resistant gene ST4 is as shown in SEQ ID NO.2. The amino acid sequence of the rice flooding-resistant protein encoded by the rice flooding-resistant gene ST4 is shown as SEQ ID NO.3. By adopting the rice flood-tolerant gene ST4 and the application thereof, the rice flood-tolerant gene ST4 is over-expressed in a rice plant, the germination rate and the seedling rate of rice can be obviously improved, and a foundation is laid for cultivating flood-tolerant rice.

The invention belongs to the technical field of plant gene engineering, and particularly relates to an OsNUC1 gene and application of a gene mutant constructed by the OsNUC1 gene to regulation and control of rice plant height, leaf length and panicle type. The regulation is to regulate the plant height and leaf growth of the rice and influence the shape of the ear part. Compared with the prior art, the OsNUC1 gene mutant has the following advantages: (1) the OsNUC1 is directionally edited by utilizing a CRISPR / Cas9 gene editing technology, an OsNUC1 gene mutant rice material is obtained, the biological phenotype of the OsNUC1 gene mutant rice material is investigated, the phenotype investigation on T1-generation seedlings of the OsNUC1 shows that the plant heights and the leaf lengths of one-week-old and two-week-old seedlings of the mutant are obviously higher than those of wild seedlings, the leaf widths have no obvious difference, and the OsNUC1 gene mutant rice material has a good application prospect; the result shows that the OsNUC1 can regulate the plant height and the leaf growth of the rice and influence the shape of the ear part. (2) it is proved that the nucleolin gene OsNUC1 is negatively regulated by a transcription factor OsARF7, and the phenotypes such as the plant height, the leaf length, the grain length and the grain width of the OsNUC1 / OsARF7 mutant are more obvious than those of the OsNUC1 mutant. The invention further proves the application of the OsNUC1 gene in regulation and control of rice plant height and leaf growth.

The invention discloses an application of a rice high environment temperature response gene OsTOGR4. According to the invention, a key factor for regulating and controlling the growth and development of a rice plant at the high environment temperature is identified for the first time, and a method for cultivating the rice adapting to the high environment temperature by mutating the OsTOGR4 gene is disclosed, so that gene resources and a research basis are provided for cultivating excellent rice varieties adapting to the high environment temperature.

The invention discloses an application of an OsST1 gene in regulating and controlling the salt tolerance of rice. Belongs to the technical field of plant genetic engineering. The invention provides the application of the OsST1 gene in regulation and control of the salt tolerance of the rice, the effect of the OsST1 gene in the salt tolerance of the rice is proved for the first time, and research finds that overexpression of the OsST1 gene can improve the salt tolerance of a rice plant and improve the survival rate of the rice plant under salt stress, which indicates that the OsST1 gene has the effect of regulating and controlling the salt tolerance of the plant, and the application of the OsST1 gene in the regulation and control of the salt tolerance of the rice plant. The adverse effect of soil salinization on rice growth in practical application is solved, and meanwhile, biological function verification of the gene also has important reference significance for rice salt tolerance molecular mechanism research.

The invention relates to the field of intelligent agriculture, in particular to a high-salt-tolerance rice planting management system based on image recognition, which comprises a data acquisition module, an image processing module, a growth condition detection module and an analysis management module, through the computer vision technology, close planting degree guidance and saline-alkaline degree control are intelligently provided for high-salt-tolerance rice planting, the management efficiency is improved, and the rice yield and quality are improved; according to the method, a Transformer coding method based on a window mechanism and global optimization is adopted, the recognition accuracy of the growth state of a rice plant is improved, the accuracy of analysis of the relationship between the growth condition of the rice and the salt and alkali content and the close planting degree of the rice is improved, and accurate guidance is provided for increasing the yield and quality of the rice; according to the method, a UNet network based on Mama block optimization is adopted, the influence of a field background on rice image feature extraction is removed, and the accuracy of rice growth condition-saline and alkaline content-rice close planting degree relation analysis is improved.

The invention belongs to the technical field of biology, and discloses a rice receptor-like kinase OsLTK1 and application thereof in resistance of magnaporthe oryzae. It is found for the first time that receptor-like kinase OsLTK1 positively regulates the resistance of rice to magnaporthe oryzae. The research shows that the transgenic plant of the OsLTK1 gene overexpressed in the rice has obvious resistance to the magnaporthe oryzae. Therefore, the OsLTK1 gene can be used for cultivating rice blast resistant rice varieties, and has great application value.

The invention discloses a rice disease and insect pest monitoring method and system, and relates to the technical field of disease and insect pest monitoring, and the method comprises the steps: firstly testing the resistance of different rice varieties to different disease and insect pests, then selecting an optimal rice planting variety for a planting field, and according to the disease and insect pest conditions of the planting field, determining the disease and insect pest conditions of the planting field; according to the method, a variety with relatively high resistance is selected for planting, damage of diseases and insect pests during later growth is reduced, and meanwhile, the cost of monitoring the diseases and insect pests is reduced, so that the cost of rice planting is reduced, a basis is provided for subsequent high-yield and high-quality planting of rice, and during rice planting, an image of each single rice plant is monitored, and a three-dimensional model is constructed; the defense effect of each part in each single rice plant is obtained, then abnormal parts and abnormal pest and disease damage types in the rice are confirmed, reference is provided for subsequent pest and disease damage treatment, the treatment position is accurately positioned, and the pest and disease damage monitoring and treatment effects are improved.

The present invention relates to methods for controlling or preventing infestation of a rice plant by the phytopathogenic microorganism Gibberella fujikuroi, comprising applying to a crop of rice plants, the locus thereof, or propagation material thereof, a compound according to formula (I) wherein R1, R2, R3, R4, R5, Y, A, B are as defined herein.

The utility model belongs to the technical field of rice planting, and particularly relates to a lodging-resistant device for rice planting, which comprises a support column, a winding piece, a locking piece and an inhaul cable, a winding roller of the winding piece is rotatably connected to the upper end of the support column through a bearing, and a driving rod is arranged at the position of a first check block arranged on the upper portion of the winding roller. A locking piece is arranged on a supporting column on the lower portion of the winding roller, a sliding sleeve is connected to a fixing sleeve of the locking piece in a sliding mode, and a stop lever fixedly connected to the upper end of the sliding sleeve abuts against a second stop block arranged on the lower portion of the winding roller. The supporting columns are used for fixing the winding rollers for winding the inhaul cables to the two ends of a row or a row of plants, the ends of the two inhaul cables are fixedly connected together, the inhaul cables are arranged on one sides of the rice plants in a lap joint mode, the rice plants are effectively blocked and supported, and the rice plants are prevented from lodging on the ground under the action of external factors such as wind and rain. The rice lodging-resistant device is simple in structure, can effectively prevent rice plants from lodging, and is convenient to use.

The invention discloses a rice growth image real-time acquisition method. The method comprises the following steps: real-time data acquisition; processing data; carrying out data visualization and analysis; according to the invention, through collection and fusion of multi-dimensional data, in combination with image analysis and a machine learning algorithm, key characteristics of rice growth are extracted; field environment data and rice plant height information are collected in real time, time sequence characteristics of environment parameters and plant height parameters are extracted based on a long-short-term memory network model, a long-term dependency relationship is captured, and accurate prediction of the rice plant height is achieved. A prediction result can be visually analyzed through a thermodynamic diagram and the like, and the growth condition of the rice is visually displayed; through a large number of trained long-short-term memory network models, precise prediction of the rice plant height can be realized, and during subsequent implementation of acquisition of rice growth images, dependence on manual observation or regular shooting is not needed, so that real-time predictability of rice growth is improved.

The invention discloses an application of a rice heat shock protein HSP101 and a coding gene thereof in rice heat-resistant breeding, and belongs to the technical field of gene engineering. The rice heat shock protein HSP101 is identified, the amino acid sequence of the rice heat shock protein HSP101 is shown as SEQ ID NO. 2, and the nucleotide sequence of the coding gene HSP101 is shown as SEQ ID NO. 1. The invention provides an application of a rice heat shock protein HSP101 and a coding gene HSP101 in heat-resistant breeding. Transgenic experiment results show that the survival rate of a rice plant overexpressed with the HSP101 gene after high temperature stress is significantly higher than that of a control plant, that is, the overexpressed HSP101 gene significantly improves the tolerance of rice to high temperature, and the HSP101 gene positively regulates the heat resistance of rice. The invention lays a foundation for stress-resistant breeding of rice, and has great economic benefits and wide application prospects for rice production.

The invention discloses a rice irrigation device and method based on layered soil moisture content sensing. The device comprises a water storage tank, a rainwater collector, a water conveying pipe, a table top, a water pump, a water outlet pipe, a hydraulic rotary spraying rod, an electromagnetic valve, a control box, a water inlet pipe, a layered soil sensor and a weather receiver. The method comprises the following steps: monitoring soil moisture content data of different depths in a root system area in real time by adopting a layered soil sensor, and collecting environmental meteorological parameters by utilizing a meteorological fusion device; the control box adopts a multi-factor dynamic weight decision-making algorithm to comprehensively analyze the soil moisture content and meteorological conditions and generate an intelligent irrigation decision-making instruction; accurate irrigation operation is achieved by controlling executing mechanisms such as a water pump, a hydraulic rotary spraying rod and an electromagnetic valve. By using the device, the irrigation amount and the irrigation time are accurately controlled, and the differentiated requirements of rice for water in different growth stages are met. The method can be widely applied to the field of intelligent agriculture.

The invention relates to application of rice genes SPPL1 and / or SPPL2 in improving high-temperature resistance of rice. Specifically, rice genes SPPL1 and / or SPPL2 participating in positive regulation and control of high-temperature stress resistance are cloned in rice, so that the rice genes SPPL1 and / or SPPL2 are overexpressed, and compared with a wild plant, the rice gene SPPL1 and / or SPPL2 overexpressed plant shows high-temperature-resistant characteristics, and agronomic characters (such as maturing rate, thousand seed weight, single-plant yield, plot yield and the like) are remarkably improved. Through cloning and discovery of new functions of the rice gene SPPL1 and / or SPPL2, a new gene target and a new resource are provided for improving high-temperature resistance genetic breeding of rice.

As a main grain crop in the world, the yield and quality of rice are often threatened by bacterial leaf blight and rice blast. According to the invention, a recombinant expression vector for expressing STTM-miR169d / p is constructed by starting from regulation and control of the expression quantity of miRNA through an STTM technology. By adopting an agrobacterium tumefaciens-mediated rice protoplast transformation technology, a rice plant containing the STTM-miR169d / p transgenic fragment is obtained. Through field inoculation of xanthomonas oryzae pv. Oryzae and inoculation of rice blast spores on in-vitro leaves, it is verified that the STTM-miR169d / p transgenic rice has significantly improved resistance to xanthomonas oryzae pv. Oryzae and rice blast compared with wild type rice. The invention not only provides a new germplasm resource for cultivating disease-resistant rice, but also provides a new thought for regulating and controlling the disease resistance of rice from the aspect of miRNA.

The invention relates to the technical field of rice planting, in particular to a rice plant protection machine which comprises a frame body, a first connecting frame is fixedly installed on one side of the frame body, a first rotating roller is rotatably installed on the first connecting frame, a second connecting frame is rotatably installed on the other side of the frame body, and a second rotating roller is rotatably installed on the second connecting frame. A placement box is arranged at the top of the middle of the frame; the extrusion assembly is arranged in the placement box, and the extrusion assembly is used for extruding the fertilizer in the placement box; the opening and closing assembly comprises an opening and closing module and an adjusting module, the opening and closing module is used for controlling fertilizer application, and the adjusting module is used for adjusting the opening and closing interval time of the opening and closing module. Compared with the prior art, by arranging the opening and closing module, the fertilizing precision is improved, the differentiated fertilizing requirements of different plots and crops are met, meanwhile, the risk of equipment shutdown caused by fertilizer blockage is reduced, and the overall operation efficiency is improved.

The invention relates to the technical field of rice phenotype extraction, and discloses a rice fine phenotype extraction method based on three-dimensional vision, a terminal and a storage medium. The method comprises the following steps: acquiring a multi-view image of a rice plant by using a depth camera, and performing camera attitude estimation on the multi-view enhanced image to obtain camera internal and external parameters corresponding to each view; constructing a neural radiation field network, training the neural radiation field network by taking the enhanced image, the contour mask and internal and external parameters of the camera as input, and learning color and density distribution of a scene by utilizing a volume rendering technology; extracting surface points based on a mixed threshold strategy by using the trained neural radiation field network, and generating a rice three-dimensional point cloud; inputting the rice three-dimensional point cloud into a point cloud instance segmentation network, and outputting an instance mask containing each organ of the rice and a segmentation result of a three-dimensional bounding box; and counting phenotypic parameters of the rice according to a segmentation result. According to the method, the fine instance segmentation of the high-density point cloud is realized while the reconstruction precision is ensured, and the phenotypic analysis of the spike fraction is supported.

The invention discloses a Chimonobambusa chensinensis CuCCoAOMT1 gene as well as a protein and application thereof, belongs to the technical field of plant genetic engineering, and particularly relates to a Chimonobambusa chensinensis caffeoyl-coenzyme A-O-methyltransferase CuCCoAOMT1 gene, and a nucleotide sequence of the CuCCoAOMT1 gene is shown as SEQ ID NO.1. The invention further discloses a preparation method of the CuCCoAOMT1 gene. The invention further discloses application of the CuCCoAOMT1 gene in regulation and control of the drought resistance of gramineous plants. By overexpressing the caffeoyl-coenzyme A-O-methyltransferase CuCCoAOMT1 gene in the rice, the lignin content in the rice can be effectively increased, the thickening of rice cell walls is promoted, the drought resistance of rice plants is improved, and a new gene resource is provided for plant material property improvement gene engineering.

The invention discloses a rice blast prevention and control and fertilizer efficiency improvement dual-objective optimization method, and relates to the technical field of agricultural informatics, and the method comprises the following steps: S1, data collection: obtaining rice field environment parameters, rice plant physiological parameters and pathogenic bacteria infection pressure indexes in real time; s2, constructing a double-objective collaborative optimization model: based on the data in the step S1, establishing a dynamic decision-making model which takes the maximization of the fertilizer efficiency utilization rate and the minimization of the rice blast occurrence risk as a combined objective. According to the method, a dynamic decision-making model taking maximization of the nitrogen fertilizer utilization rate and minimization of the rice blast severity as a combined target is constructed, the nitrogen fertilizer application amount, the bactericide type and the time sequence are subjected to collaborative optimization, a weight coefficient is introduced into the model, the target priority is dynamically adjusted according to the growth period, and the nitrogen fertilizer application amount is restrained in an agronomic safety interval; diseases caused by excessive application of nitrogen are avoided, positive correlation between the nitrogen fertilizer and the diseases is reduced from the source, pesticide dependence is reduced, and double improvement of the control effect and the fertilizer effect is achieved.

The invention discloses a method for relieving high-temperature heat damage to rice, and relates to the technical field of rice cultivation. The method for relieving the high-temperature heat damage to the rice comprises the steps that when the rice suffers from high-temperature heat damage stress in the heading and flowering period, a first conditioning agent is sprayed to rice plants, and a second conditioning agent is sprayed before the first conditioning agent is applied; the first conditioning agent contains methyl jasmonate with the concentration of 1-4 mmol / L, tannic acid with the concentration of 10-40 mg / L, calcium nitrate with the concentration of 6-20 mg / L and betaine with the concentration of 12-30 mg / L; the second conditioning agent is mainly formed by mixing propylene glycol and humic acid according to the weight ratio of 1: (10-20). The problems that the effect of relieving high-temperature heat damage by independently using methyl jasmonate is unstable and the efficiency is not high are solved.

The invention discloses a heavy metal blocking agent special for rice and a method for reducing the cadmium content of rice grains by using the heavy metal blocking agent, and belongs to the technical field of agricultural environment protection and polluted soil remediation. The heavy metal blocking agent special for rice comprises the following raw materials: 20-30% of molybdenum disulfide, 10-20% of diammonium hydrogen phosphate, 5-10% of disodium magnesium ethylene diamine tetraacetate, 5-10% of zinc ethylene diamine tetraacetate, 10-20% of a silicon fertilizer, 1-2% of a cosolvent, 1-2% of a solubilizer, 1-2% of ethanol and the balance of water. The special heavy metal blocking agent for the rice can effectively inhibit heavy metal cadmium from being transferred into rice grains, obviously reduce the cadmium content of the rice grains, obviously improve the yield of the rice grains, and can obviously reduce the cadmium content in the rice and ensure that the cadmium content in the rice grains meets the national food hygiene standard when being applied to the rice planted in a rice field with soil polluted by cadmium.

The application provides a method for increasing the length of a main panicle of rice, the number of secondary branches of the main panicle, the number of grains on the main panicle of rice and / or improving the yield of rice, which comprises overexpressing a cystathionine beta-lyase gene OsCBL in rice by using a recombinant vector containing a CDS coding sequence of the cystathionine beta-lyase gene OsCBL as shown in SEQ ID NO: 2 to obtain a transgenic plant. The transgenic plant obtained by the method has an increased number of grains per panicle and an increased yield per plant compared with normal plants. The method can be used for treating rice plants to improve the yield of rice and provides a new gene resource and a new idea for breeding rice with increased yield.

A transgenic rice plant contains a recombinant DNA construct in its genome, the construct comprising a first nucleic acid having a sequence of a first Golden 2-like transcription factor (GLK) gene operably linked to its native promoter and 5' untranslated region (5' untranslated region, 5' UTR), and a second nucleic acid having a sequence of a second GLK gene operably linked to its native promoter and 5' UTR, the second GLK gene being different from the first GLK gene. The first GLK gene and the second GLK gene are both from C4 plants, and compared to untransformed wild-type rice plants, the transgenic rice plant increases shoot biomass by 65-106% and grain yield by 50-95%. A method for producing a transgenic rice plant and a recombinant DNA construct that can be used in the method are also provided.