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Plant having improved resistivity or sensitivity to 4-hppd inhibitor

一种4-HPPD、抑制剂的技术，应用在对4-HPPD抑制剂的抵抗性或敏感性提高了的植物领域，能够解决尚未开发出等问题

Active Publication Date: 2013-11-20
NAT AGRI & FOOD RES ORG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, technologies capable of effectively accomplishing these objectives have not yet been developed

Method used

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  • Plant having improved resistivity or sensitivity to 4-hppd inhibitor
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  • Plant having improved resistivity or sensitivity to 4-hppd inhibitor

Examples

Experimental program
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Effect test

Embodiment 1

[0220] (Example 1) Specificity of 4-HPPD inhibitor resistance locus

[0221] Among japonica rice species, no species sensitive to BBC, which is a type of 4-HPPD inhibitor, has been known. On the other hand, BBC sensitivity sometimes appears in rice varieties bred by crossing japonica and indica.

[0222] Then, QTL analysis of the Koshihikari / Habataki chromosome segment replacement system (KHSL) using the BBC-resistant rice variety "Koshihikari" and the sensitive rice variety "Habataki" was performed as described above. As a result, only the short arm region of the second chromosome was replaced with Habataki-type KHSL (KHSL04) showing sensitivity, so it was clarified that the locus determining BBC resistance was located on the short arm of the second chromosome of Koshihikari (see Table 2). In addition, Table 2 shows a part of the results of the QTL analysis of KHSL. In addition, "A" in Table 2 indicates that the marker is derived from Koshihikari, and "B" indicates that the...

Embodiment 2

[0231] (Example 2) Identification of 4-HPPD inhibitor resistance genes

[0232]As described above, it was suggested by QTL analysis that the genes determining resistance to 4-HPPD inhibitors are present on the short arm of chromosome 2 in rice. Therefore, attention was paid to the gene presumed to be the iron / ascorbate-dependent oxidoreductase gene located at this locus, and the linkage relationship between the phenotype (BBC high sensitivity) and the genotype was clarified while testing the Tos17 insertion system. A recombinant of this gene was introduced into BBC-sensitive A. thaliana and rice, and the effect of conferring BBC resistance was investigated.

[0233] That is, in the BBC resistance-determining locus specified by QTL analysis, there is a gene estimated to be an iron / ascorbate-dependent oxidoreductase like the HPPD enzyme whose activity is inhibited by the BBC (hereinafter also referred to as "target gene"). ”) (refer to Table 4).

[0234] Table 4

[0235]

...

Embodiment 3

[0243] (Example 3) Determination of Resistance or Sensitivity to 4-HPPD Inhibitor Using Base Sequence Analysis of HIS1 Gene

[0244] The HIS1 gene located on chromosome 2 of Japanese type rice was identified, but when PCR was performed to amplify the HIS1 gene, an amplified product was also obtained in BBC-sensitive rice varieties. In addition, among BBC-sensitive rice varieties, kasaras may be difficult to judge sensitivity and resistance according to BBC treatment conditions. Therefore, whether or not the nucleotide sequence of the HIS1 gene is related to the degree of BBC sensitivity can be determined by PCR to amplify a specific region of the HIS1 gene was investigated.

[0245] In addition, although not shown in the figure, Momiroman, Takanari, and Kasaras are all BBC-sensitive varieties, but their degrees are different, and it was confirmed that Momiroman and Takanari showed stronger BBC sensitivity than Kasaras.

[0246] Therefore, first, BBC-sensitive varieties (Momil...

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Abstract

A 4-HPPD inhibitor-resistant gene is identified as a gene (HIS1 gene) which is estimated as an iron- / ascorbic acid-dependent oxidoreductase gene located on the short arm of chromosome-2 of a rice plant, by carrying out QTL analysis using a 4-HPPD inhibitor-sensitive rice plant and a 4-HPPD inhibitor-resistant rice plant or the like. It is also found that a homologous gene (HSL1 gene) to the HIS1 gene is located on chromosome-6 of a rice plant. It is found that a plant having improved resistivity or sensitivity to a 4-HPPD inhibitor can be produced with high efficiency and the resistivity or sensitivity of a plant to a 4-HPPD inhibitor can be determined with high efficiency using the aforementioned genes.

Description

technical field [0001] The present invention relates to agents for imparting resistance or sensitivity to 4-HPPD inhibitors to plants, transformed plant cells capable of regenerating plants with increased resistance or sensitivity to 4-HPPD inhibitors, and the cells Regenerated plant bodies, and methods for their manufacture. In addition, the present invention relates to a method for determining the resistance or sensitivity of a plant to a 4-HPPD inhibitor, and a method for breeding a plant having improved resistance or sensitivity to a 4-HPPD inhibitor using the determination. Background technique [0002] As the demand for bioethanol fuel increases, the import price of feed grains from overseas has risen, putting pressure on domestic livestock operations. Therefore, in order to increase the domestic production and self-sufficiency rate of feed, the cultivation of various crops other than rice using fallow fields and the like has been carried out. However, paddy fields s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82A01H1/00A01H5/00C12N5/10C12Q1/68
CPCC07K14/415C12N15/8274A01H1/02C12N9/0004C12Q1/6895A01H5/00
Inventor 加藤浩前田英郎春原嘉弘安东郁男大岛正弘川田元滋吉田均广濑咲子川岸万纪子谷口洋二郎村田和优前田宽明山田祐司关野景介山崎明彦
Owner NAT AGRI & FOOD RES ORG
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