Application of nitrate radical transporter gene OsNRT1.8 in rice breeding

Abstract

The invention discloses application of a nitrate radical transporter gene OsNRT1.8 in rice breeding and belongs to the field of plant genetic engineering. The protein encoding amino acid sequence of the gene OsNRT1.8 is shown as SEQ ID NO.1, and a cDNA sequence is shown as SEQ ID NO.2. By establishing rice gene OsNRT1.8 overexpression plant and a gene OsNRT1.8 interference plant, it is found that normal rice tiller number and spike number of each plant can be increased by improving gene OsNRT1.8 expression. Therefore, the gene OsNRT1.8 can be used for increasing rice yield in rice breeding. The gene OsNRT1.8 has an important application value on the aspects of elaboration about nitrogen influence on plant growth and development and improvement of rice plant type.

Description

technical field

[0001] The invention belongs to the field of plant genetic engineering, in particular to nitrate transport genes OsNRT1.8 Application in rice breeding. Background technique

[0002] China's rice planting area accounts for 20% of the world's total crop planting area, but nitrogen fertilizer application accounts for 37% of the world's total application amount; in 1995, China's nitrogen fertilizer production and use amount reached the first in the world, but nitrogen fertilizer use efficiency is low Low, the amount of nitrogen fertilizer application has increased by 20 times compared with 50 years ago. According to this trend, it is expected to triple again by 2050. Excessive application of nitrogen fertilizer will lead to ecological pollution problems such as eutrophication of water [Xu Guohua, Fan Xiaorong. Rice Nitrate Transporter Gene OsNRT1.1a and OsNRT1.1b functional research. Nanjing Agricultural University, 2011: 4-6]. More nitrogen nutrients are...

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