26 results about "Transporter gene" patented technology

The invention discloses a method for constructing a high-yield natamycin engineering strain through overexpression of an extracellular transport protein gene pimA and a pathway specific regulation gene pimR, and the high-yield engineering strain is applied to fermentation production of natamycin. The yield of the natamycin can reach 19.49 g / L through fed-batch fermentation in a fermentation tank of 5L of the high-yield strain S.ilvosporeus pimA-pimR, and the yield of the natamycin is improved by 33.12% compared with the yield of a chassis strain S.ilvosporeus TUST01; the production intensity is improved by 33.33% compared with the production intensity of a chassis strain S.ilvosporeus TUST01, and the strain has a relatively high industrial application value.

This invention provides compositions and treatment methods for reducing hyperphenylalaninemia. [Solution] A genetically modified bacterium is provided, comprising: a) one or more genes encoding phenylalanine ammonia lyase (PAL), which are operably linked to a promoter that is not naturally associated with the PAL gene and can be directly or indirectly induced; b) one or more genes encoding a phenylalanine transporter, which are not naturally associated with the phenylalanine transporter gene and can be operably linked to a promoter that is directly or indirectly induceable; and c) one or more genes encoding mutant fumarate and nitrate reductase (FNR), which are not naturally associated with the FNR gene and can be operably linked to a promoter that is directly or indirectly induceable.

The invention discloses a genetically engineered bacterium for synthesizing L-glutamine by taking glucose as a carbon source and application of the genetically engineered bacterium. The genetically engineered bacterium takes escherichia coli as a chassis strain; according to the invention, a citrate synthase gene gltA, an aconitic acid hydratase gene can, an isocitrate dehydrogenase gene icd, a glutamate dehydrogenase gene gdh, a glutamine synthetase gene glnA and ammonium transporter genes amt and amtb can be overexpressed. Compared with the prior art, the invention provides a new strain and a new method for biosynthesizing L-glutamine, and the new strain and the new method have the significances of economy, scientificity, low cost, simple process and large-scale production.

The application discloses an Escherichia coli transmembrane transporter gene and application thereof in vitamin B6 biosynthesis. The application weakens a vitamin B6 transporter gene coding in the Escherichia coli, and the ability of a genetically engineered strain to produce vitamin B6 is greatly improved, and the genetically engineered strain has great application and popularization value. In the best embodiment, the extracellular yield of vitamin B6 of the genetically engineered strain is increased by 33.7% compared with that of a wild type, and the effect is extremely remarkable.

This invention belongs to the field of crop breeding, specifically relating to an NtLHT1 gene and its application in tobacco's resistance to low nitrogen stress. It involves artificially intervening in the mutation of specific nucleotide sequence fragments in the NtLHT1 gene to obtain two mutated nucleotide sequence fragments, resulting in a mutant NtLHT1 gene, and the application of the mutant NtLHT1 gene in improving tobacco's resistance to low nitrogen stress. The NtLHT1-edited tobacco plants of this invention exhibit enhanced absorption of nitrate and ammonium nitrogen during their vigorous growth period, increased activity of nitrate reductase and glutamine synthase, increased expression of nitrogen transporter genes, and improved nitrogen utilization efficiency. This invention increases the activity of nitrate reductase and glutamine synthase, thereby improving tobacco's resistance to low nitrogen stress and providing a genetic resource and material basis for the development of nitrogen utilization-related genes and strains in tobacco.

The invention belongs to the technical field of insecticides, and particularly relates to a ds-ABC transporter gene for preventing and controlling resistant Frankliniella occidentalis as well as a screening method and application of the ds-ABC transporter gene. The ABC transport protein gene is selected from any one or more of ABCG20, ABCA1, ABCB8, ABCD2 and ABCA5, and the ABC transport protein gene is selected from any one or more of ABCG20, ABCA1, ABCB8, ABCD2 and ABCA5. The screening method comprises the following steps: (1) testing the resistance level of the Frankliniella occidentalis to spinetoram, imidacloprid and thiamethoxam; (2) extracting and purifying the total RNA of the Frankliniella occidentalis population by using a trace total RNA extraction kit, synthesizing cDNA (complementary deoxyribonucleic acid) by taking the RNA as a template, and then carrying out quantitative primer design and gene expression; and (3) carrying out significant analysis on difference analysis of all data through real-time fluorescent quantitative detection, and screening out 5 ds-ABC transporter genes which are subjected to significant up-regulation expression in a resistant population. According to the invention, the key ABC transporter gene for mediating the resistance of the Frankliniella occidentalis to the spinetoram is screened and determined, and a basis is provided for development of a novel anti-resistance insecticide taking the ABC transporter as a target.

The invention discloses a promoter of an alfalfa malic acid transport protein gene MsALMT1 and application of the promoter. The nucleotide sequence of the promoter is shown as SEQ ID No.1. The invention further discloses a preparation method of the promoter. The invention provides the nucleotide sequence of the alfalfa malic acid transporter gene MsALMT1 promoter, and the alkali resistance of the plant can be obviously improved after the promoter drives the overexpression of MsALMT1 in alfalfa, so that a gene resource is provided for the improvement of the alkali resistance of the plant, and the application value is wide.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a zostera marina magnesium ion transporter gene ZosmaMGT10, a recombinant vector and application. The CDS full length of the gene is 1239 bp, 412 amino acids are encoded, and the gene has a conservative CorA structural domain. It is proved for the first time that ZosmaMGT10 protein has Mg < 2 + > transport activity and can be specifically combined with Mg < 2 + > through E299, E300 and E303 residues, abscisic acid can remarkably induce expression of the ZosmaMGT10 protein in zostera marina leaves, the Mg < 2 + > absorption capacity of zostera marina is enhanced, and then plant stress resistance is improved. The gene ZosmaMGT10 provided by the invention can be used as a key candidate gene, is used for cultivating marine plant varieties with high Mg < 2 + > absorption efficiency and strong stress resistance, and provides a new tool and a theoretical basis for ecological restoration of coastal zones and breeding of stress-tolerant crops.

The invention discloses an application of a rice phosphorus transporter gene and an encoding protein thereof in improving nutrient utilization efficiency and rice quality. According to the invention, OsPHT1 is mutated or inhibited; 1 gene expression can significantly improve nutrient utilization efficiency and rice taste quality and nutritional quality. The method is mainly embodied in that accumulation of nitrogen and phosphorus nutrients of rice is remarkably reduced, the total protein content, glutelin content, prolamin content, globulin content, albumin content and phytic acid content of rice grains are remarkably reduced, the gel consistency of the rice is increased, the reduction value is reduced, and the eating quality and the nutritional quality of the rice are further improved.

The invention discloses a method for enhancing cholesterol uptake of saccharomyces cerevisiae, and belongs to the technical field of bioengineering. The method comprises the following steps: knocking out transcription inhibition factors MOT3 and ROX1 of genes related to cholesterol uptake and transport of saccharomyces cerevisiae; the transcription activating factors SUT1 and UPC2-1 of the cholesterol transporter gene are over-expressed; expression of sterol esterifying enzyme ARE2 is enhanced, so that uptake and storage of exogenous cholesterol by the saccharomyces cerevisiae are further enhanced. According to the method disclosed by the invention, the uptake and storage of cholesterol by the saccharomyces cerevisiae are enhanced, and an important reference is provided for construction of a steroid hormone synthesis chassis taking cholesterol as a substrate.

The invention discloses application of a monosaccharide transporter gene OsSTP28 in improvement of rice plant height and lodging resistance, provides application of a green and friendly rice gene OsSTP28 in improvement of rice plant height and lodging resistance, and discovers that knockout of the rice OsSTP28 gene can reduce rice plant height and increase rice lodging resistance.

The application provides a Chlamydomonas reinhardtii ABC transporter gene CrABCG, a coding protein and application thereof, and the gene comprises CrABCG3 or CrABCG5, and the gene sequence comprises SEQ ID NO:1 or SEQ ID NO:2. It is found by the application that the CrABCG protein is related to carbon and nitrogen metabolism of Chlamydomonas reinhardtii, and preliminary researches show that the expression of the CrABCG3 gene of Chlamydomonas reinhardtii is increased after nitrogen limitation, while the expression of the CrABCG5 gene is first decreased and then increased, and the results show that the CrABCG protein influences the transport, synthesis and accumulation of lipid substances. Therefore, the engineering algae strains with high lipid content can be obtained by screening and modification of the algae strains with high expression of the CrABCG gene.

The invention discloses a construction method and application of escherichia coli genetically engineered bacteria for producing malic acid by synergistically utilizing CO2, and belongs to the technical field of bioengineering. According to the invention, an L-malic acid chassis strain MG1655W (DE3) constructed by a gene editing technology in a laboratory is used as an initial strain, a lactic dehydrogenase encoding gene ldhA and a phosphate acetyltransferase encoding gene pta in escherichia coli are knocked out on the basis, the production paths of lactic acid and formic acid are blocked, and the yield of lactic acid and formic acid is increased. The method comprises the following steps of: firstly, amplifying a phosphoenolpyruvate carboxylase gene ppc and a CO2 transporter gene CA, constructing a recombinant plasmid, introducing the recombinant plasmid pACYCDuet-1-CA-ppc into an expression host MG1655W (DE3) delta ldhA delta pta, and screening to obtain the engineering bacterium MG1655W (DE3) delta ldhA delta pta-CA-ppc by overexpressing the ppc gene and the CA gene and improving the CO2 immobilization and concentration capability of escherichia coli. The obtained engineering bacterium is subjected to cell proliferation under an aerobic condition and malic acid accumulation under an oxygen limiting condition, the final malic acid yield is increased to 140-212%, and the engineering bacterium has important industrial application value.

Provided are a genetically engineered bacterium for producing lacto-N-tetraose, a preparation method therefor, and use thereof. The genetically engineered bacterium overexpresses an exogenous transporter gene BmSet. The genetically engineered bacterium can enable lacto-N-tetraose to be quickly and efficiently excreted out of cells, promote the increase in the fermentation yield of lacto-N-tetraose, and increase the concentration of lacto-N-tetraose in extracellular media, thereby having good industrial application prospects.

The application discloses a strain of Concretic Hendonexis CCFM1541 capable of promoting intestinal mineral absorption and improving intestinal flora, and a postbiotic thereof, and belongs to the technical field of microorganisms for promoting zinc and calcium absorption and improving intestinal flora. The Concretic Hendonexis CCFM1541 in the application can promote the increase of zinc and calcium absorption of mice, and improve intestinal flora, and the specific embodiments are as follows: (1) restoring the organ index, serum zinc and ALP content of mice, and regulating the expression of zinc-related transport genes in the small intestine; (2) improving the serum calcium and ALP content of mice, restoring the length, diameter and weight of femur, and effectively promoting bone formation and optimizing the trabecular microstructure; (5) effectively reversing the decrease of intestinal flora diversity and structural disorder induced by antibiotics, and restoring the intestinal microecological homeostasis. Therefore, the Concretic Hendonexis CCFM1541 has great application prospect in promoting intestinal mineral absorption and regulating intestinal flora.

This invention belongs to the field of fungal genetic engineering technology and discloses the ABC transporter gene of shiitake mushroom. LeABC2 And its application in regulating the heat tolerance of shiitake mushrooms. Genome-wide association analysis was performed based on the heat tolerance phenotypic data of 133 shiitake mushroom strains to identify candidate genes regulating heat tolerance. LeABC2 , build LeABC2 The gene function was studied using an RNA interference vector via Agrobacterium-mediated genetic transformation. The results showed... LeABC2 The gene negatively regulates the heat resistance of shiitake mushroom mycelium, and interfering with the expression of this gene can improve the heat resistance of mycelium, providing a new solution to the problem of high temperature affecting yield and quality in the shiitake mushroom industry.

The invention relates to an application of a transporter and a gene thereof in improving tolerance of filamentous fungi to ionic liquid, and belongs to the technical field of genes. The invention aims to solve the problems that the stability of filamentous fungi in ionic liquid is poor at present, so that a fungus and ionic liquid synergistic technology is difficult to effectively apply and the like. The invention provides a transporter for improving tolerance of fungi to ionic liquid and a gene of the transporter. The nucleotide sequence of the transporter gene is shown as SEQ ID NO.1; the amino acid sequence of the transporter is as shown in SEQ ID NO. 2. After gene overexpression of the transporter provided by the invention, tolerance of filamentous fungus aspergillus terreus to ionic liquid is improved, including improvement of growth ability of a strain in the ionic liquid and improvement of activity of cellulase and hemicellulase of the strain in the ionic liquid.

The present application relates to a kind of recombinant coryneform bacteria and its method for fermenting production of spermidine with glucose as raw material.The present application first knocks out snaA gene in the genome of coryneform bacteria, constructs knockout strain SP-1.Spermidine transporter gene cluster, S-adenosylmethionine synthetase, spermidine synthase, ornithine decarboxylase, S-adenosylmethionine decarboxylase derived from Escherichia coli are expressed in previously constructed knockout strain SP-1 using shuttle plasmid pEC-XK99E and pXMJ19 plasmid between Escherichia coli and coryneform bacteria as expression vector.The recombinant coryneform bacteria of the present application can directly utilize glucose to directly produce spermidine.In the absence of exogenous methionine, 2.61g / L spermidine can be accumulated in 250mL shake flask fermentation for 72h.Spermidine yield can reach 45.2g / L in 5L fermentor by substrate feeding.

The invention discloses a traditional Chinese medicine and western medicine combined medication safety intelligent evaluation system and method, and belongs to the technical field of medicine safety evaluation. The system comprises a traditional Chinese medicine component analysis module, a hypergraph convolution prediction module, a medicine effect collaborative evaluation module and an individualized decision module; a quaternary hypergraph network of traditional Chinese medicine components-western medicine-metabolic enzyme-target spots is constructed, and a hypergraph convolutional neural network and a graph attention mechanism are adopted to predict pharmacokinetic interaction; evaluating pharmacodynamic interaction by constructing a target-pathway double-layer network, and establishing a closed-loop feedback collaboration mechanism of pharmacokinetics and pharmacodynamics; cYP450 enzyme system gene polymorphism and transporter gene variation information of a patient are integrated, individualized medication decision and dosage adjustment are achieved, the problems that in the prior art, multi-component multi-target interaction prediction is not accurate, pharmacokinetics-pharmacodynamics collaborative analysis is lacked, and the individualized guiding ability is insufficient are solved, and the application prospect is wide. And the safety and effectiveness of combination of Chinese and western medicines are remarkably improved.

The application belongs to the technical field of genetic engineering, and particularly relates to a transport gene NtABCG6 gene and application thereof in promoting genetic transformation efficiency of multiple species. The nucleotide sequence of the NtABCG6 gene is shown as SEQ ID No. 1. By expressing the NtABCG6 gene, the transition of callus to bud differentiation can be significantly accelerated, and the period from agrobacterium infection to obtaining a rooting positive plant is shortened. Stable regeneration promotion effects are exhibited in Nicotiana tabacum, tomato and potato, and a new strategy is provided for constructing a high-efficiency, stable and low-intervention plant genetic transformation system.

The invention belongs to the technical field of biological medicine, and particularly relates to application of exosome miRNA as a gastric cancer diagnosis marker and a treatment target. The regulation and control effects of circulating exosome miRNA in early gastric cancer diagnosis and malignant progression are systematically analyzed, and it is found that miR-412-5p is remarkably low in expression in early gastric cancer. The scheme of the invention further confirms that the nucleoplasm transporter gene XPO1 is a direct target gene of miR-412-5p, and activation of a PI3K-AKT pathway can be driven by expression imbalance of the nucleoplasm transporter gene XPO1.

The application discloses a corn sugar transport gene ZmSTP24 and application thereof. The nucleotide sequence of the corn ZmSTP24 gene is shown as SEQ ID NO. 1, and the amino acid sequence coded by the gene is shown as SEQ ID NO. 2. Through CRISPR / Cas9 technology, the corn ZmSTP24 gene is edited and its function is researched. Compared with a transgenic receptor, the ZmSTP24 transgenic strain has a significantly reduced grain sucrose content, starch content and grain size. It is shown that the ZmSTP24 gene cloned in the application positively regulates the accumulation of grain sugar and other carbohydrates, and influences corn filling and grain size.

The invention discloses an application of a soybean GmPOT coding gene in resisting soybean mosaic virus diseases. The invention relates to an application of a soybean oligopeptide transporter gene GmPOT as shown in SEQ ID NO.1 in genetic engineering modification of soybean mosaic virus resistance. The over-expression of the gene can significantly improve the resistance of soybeans to soybean mosaic virus diseases. The soybean oligopeptide transporter gene GmPOT disclosed by the invention can be transformed into soybeans through genetic engineering, and the resistance of the soybeans to soybean mosaic virus diseases is finally regulated and controlled.

This invention relates to a heavy metal transporter PhHMA5I, its mutants, and its applications. The heavy metal transporter PhHMA5I is a protein as described in (1) or (2): (1) a protein encoded by the heavy metal transporter gene PhHMA5I as shown in SEQ ID No. 1, with an amino acid sequence as shown in SEQ ID No. 2; (2) a protein derived from (1) with the same function obtained by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence shown in SEQ ID No. 2. Compared with the prior art, the heavy metal transporter PhHMA5I of this invention is constructed using gene editing technology to create a knockout cassette vector and transferred into petunia W115 to form a petunia mutant. Under high zinc stress, the root elongation of this petunia mutant is significantly higher than that of wild-type petunia W115.

The present invention relates to a genetically modified rice plant or plant cell, comprising a heterologous heavy metal ATPase gene operably linked to an OsActin1 promoter: a heterologous ATP-binding cassette (ABC) transporter gene operably linked to an OsActin1 promoter, and a heterologous phytochelatin synthase gene operably linked to an OsActin1 promoter, wherein the OsActin1 promoter has low activity in a seed endosperm of the modified rice plant compared to its activity in other vegetative tissues of the modified rice plant; wherein a rice grain of said genetically modified rice plant has reduced arsenic (As) and cadmium (Cd) compared to a control rice plant that has not undergone said genetic modification. The present invention also relates to a method of creating such a genetically modified rice plant or plant cell, and a kit for doing so.

The invention provides an L-threonine production strain as well as a construction method and application thereof, a homologous recombination gene editing technology is utilized, a lysine transporter gene lysE and an isoleucine transporter gene brnF are knocked out, and firstly, threonine-inhibited lysC (T311I), hom (G378I) and thrB (A20G) are overexpressed; performing overexpression on thrC and aspB; a thrA-thrB-thrC gene and an efflux protein rhtC gene which are derived from escherichia coli are introduced in a heterologous manner and are subjected to multiple copies, wherein the thrA-thrB-thrC is subjected to synchronous multiple copies through an artificial operon Psod-thrA-thrB-thrC; the constructed strain does not contain plasmids, has no defects, does not need induction, has good hereditary stability, efficiently synthesizes L-threonine from the beginning by taking glucose as a substrate, and has high fermentation yield.