197 results about "Transporter gene" patented technology

Disclosed is a method using an alkane response element (ARE) from, e.g., Acinetobacter spp. to (i) identify and clone hydrocarbon biosynthesis genes, (ii) identify and clone hydrocarbon transporter genes (iii) identify and clone hydrocarbon response genes. Screening cells were developed that expressed a transcriptional activator, e.g., alkR, and included a reporter gene, e.g., GFP operatively linked to an ARE promoter, e.g., the alkM promoter. The cells were transformed with libraries from organisms capable of hydrocarbon biosynthesis. Transformed cells that expressed the reporter gene harbored library-derived genes involved in one or more of the above-mentioned processes; and these genes were isolated from the cells using standard molecular biology techniques. Additional systems were designed wherein screening cells also expressed a gene identified in the original screen, e.g., an additional hydrocarbon pathway gene, e.g., an enhancer.

The invention discloses a caco-2 cell model for CRISPR / CAS9-mediated drug transporter targeted knockout and a method thereof. The method is used for non-diagnostic or therapeutic purposes and comprises the following steps: designing sgRNA with target specificity for P-gp, BCRP and MRP2 transporters and constructing a sgRNA expression vector, wherein a sequence of the designed sgRNA is shown as SEQID NO. 1-6 in a sequence table; respectively designing P-gp, BCRP and MRP gene single knockout and pairwise combination double knockout by utilizing CRISPR / CAS9, co-transfecting a caco-2 cell with anhCas9 plasmid and performing monoclonal expansion culture to obtain the caco-2 cell model for transporter gene targeting. The caco-2 cell model obtained by the method disclosed by the invention has the beneficial effects that the mutual interference among different transporters is effectively eliminated, and a more specific and more sensitive cell model is provided for drug transport research.

A recombinant microorganism comprising a lactic acid (LA) transporter, wherein the expression of the LA transporter in the recombinant microorganism is increased relative to a parent microorganism, and a method of producing lactic acid using same.

The invention discloses a malic acid transporter gene for controlling organic acid content in apple pulp and application of the transporter gene, and relates to the field of apple molecule genetic breeding. The sequence of the gene is as shown in SEQ ID NO.1 and the sequence of encoded protein is as shown in SEQ ID NO.2. The application is as follows: (1) designing a molecular marker according to the sequence for marker screening in breeding; and (2) detecting and screening related acidity screening phenotypes according to the expression quantity of the gene or the sequence subtype of a transcript, or improving or reducing the expression quantity of the gene by virtue of a transgenic method so as to acquire expected pulp acidity characters. The organic acid transporter gene disclosed by the invention can be used for determining pulp acidity; on the basis of the assembling result of RNA-Seq and the annotation result of a whole genome frame diagram, the obtained gene is more accurate; and the gene can be screened at the early stage of apple breeding, so that breeding time can be saved, and meanwhile screening efficiency can be improved.

Provided are: a transporter gene involved in the promotion or inhibition of the absorption of Cd by a root; a mutant gene of the transporter gene; a transporter protein of the transporter gene; a rice plant of which the absorption of Cd is reduced; and a method for selecting and growing a rice plant of which the absorption of Cd is reduced. A gene encoding a transporter protein involved in the regulation of the absorption of cadmium, which comprises a DNA nucleotide sequence represented by SEQ ID NO: 2; a gene encoding a transporter protein involved in the regulation of the absorption of cadmium, which comprises a DNA nucleotide sequence represented by SEQ ID NO: 3; a gene encoding a transporter protein involved in the regulation of the absorption of cadmium, which comprises a DNA nucleotide sequence represented by SEQ ID NO: 4; and a transporter protein involved in the regulation of the absorption of cadmium, which comprises an amino acid sequence represented by SEQ ID NO: 1.

Provided are a transporter gene involved in the promotion or inhibition of Cd absorption by roots, a mutant gene thereof and a transporter protein thereof, as well as a rice plant in which Cd absorption is inhibited and a method for selecting and raising a Cd absorption-inhibiting rice plant, which are a gene encoding a transporter protein involved in the regulation of cadmium absorption, which contains the DNA nucleotide sequence shown in SEQ ID NO:2, a gene encoding a transporter protein involved in the regulation of cadmium absorption, which contains the DNA nucleotide sequence shown in SEQ ID NO:3, a gene encoding a transporter protein involved in the regulation of cadmium absorption, which contains the DNA nucleotide sequence shown in SEQ ID NO:4, and a transporter protein involved in the regulation of cadmium absorption, which contains the amino acid sequence of SEQ ID NO:1.

The invention discloses a tobacco cadmium transporter gene NtHMA2, and a cloning method and application thereof. The nucleotide sequence of the tobacco cadmium transporter gene NtHMA2 is disclosed as SEQ ID:No.1, and the coded amino acid sequence is disclosed as SEQ ID:No.2. The invention also discloses a cloning method of the tobacco cadmium transporter gene NtHMA2, which comprises the following steps: A. determining the NtHMA2 gene sequence; B. extracting tobacco RNA (ribonucleic acid), and carrying out reverse transcription to obtain a first chain cDNA (complementary deoxyribonucleic acid); C. designing and synthesizing specific primers according to the NtHMA2 gene sequence, and carrying out PCR (polymerase chain reaction) amplification by using the cDNA as the template; D recycling and purifying the PCR product; E. connecting the purified product with the vector, and transforming competent cells; and F. screening positive clone, carrying out PCR amplification on the positive clone, and sequencing. By regulating the expression of the tobacco cadmium transporter gene NtHMA2, the method can lower the operation rate of cadmium being transported from the tobacco root to leaves, thereby lowering the chromium content of the tobacco and providing a gene resource for green high-quality tobacco.

There are provided trehalose transporter gene and a method of introducing trehalose into cells by using the gene. Candidates for the trehalose transporter genes were searched in P. vanderplanki EST, resulting in being obtained cDNA designated as Tret1. Tret1 encodes a 504 amino acid protein with 12 trans-membrane structures. Tret1 expression was induced by desiccation stress and predominant in the fat body. Functional expression of TRET1 in Xenopus oocytes showed that transport activity was specific for trehalose and independent of extracellular pH and electrochemical membrane potential. The direction of transport of TRET1 was reversible depending on the concentration gradient of trehalose. Apparent Km and Vmax of TRET1 for trehalose were extraordinarily high values. These results indicate that TRET1 is a facilitated, high-capacity trehalose-specific transporter. Tret1 is widespread in insects. Furthermore, TRET1 conferred trehalose permeability upon cells including those of vertebrates as well as insects.

The invention discloses an engineering bacterium, and an application thereof in the production of caffeic acid, and belongs to the technical field of bioengineering. The engineering bacterium providedby the invention is a recombinant bacterium capable of producing caffeic acid at a low cost; the recombinant bacterium can simultaneously express four enzymes which are tyrosine phenol lyase, tyrosine ammonia lyase, L-lactate dehydrogenase and NADH oxidase respectively; and the recombinant bacterium knocks out a phenolic substance-decomposing gene, and can achieve enhanced expression of any one or more of a lactic acid transporter gene, a catechol transporter gene and a coenzyme synthesis-related gene. The engineering bacterium has the advantages of realization of the efficient production ofcaffeic acid, simple process, few impurities and great industrial application values.