51 results about "Transporter gene" patented technology

The invention discloses an application of a multidrug-resistant efflux transporter gene OsMRET1 in regulation and control of chalkiness traits of rice. A nucleotide sequence of a coding region of the gene is shown as SEQ ID No.1, and a coded amino acid sequence is shown as SEQ ID No.2. The invention further discloses an application of the multidrug-resistant efflux transporter gene OsMRET1 in regulation and control of chalkiness traits of rice. It is found for the first time that the gene OsMRET1 can regulate and control formation of chalkiness, an OsMRET1 gene knockout mutant is created through a gene editing technology, an OsMRET1 gene overexpression strain is obtained through a transgenic technology, it is verified that the OsMRET1 gene is a new gene for regulating and controlling rice chalkiness, and overexpression of the gene can reduce rice chalkiness and improve rice chalkiness. And a new gene resource and a technical route are provided for rice quality improvement.

The invention provides a beta-alanine producing strain as well as a construction method and application thereof. According to the bacterial strain, an acetaldehyde dehydrogenase gene adhE, a lactic dehydrogenase gene ldhA, an acetokinase gene ackA, a pyruvate dehydrogenase gene poxB, a DNA binding transcription inhibition factor coding gene lacI, an aspartate kinase thrA, a pantothenic acid synthase gene panC, an alanine synthesis transaminase gene cycA and an alanine synthesis transaminase gene yfbQ are knocked out from an E.coli W3110 genome, and the bacterial strain is obtained. A phosphoenolpyruvate carboxylase gene ppc, a pyridine nucleotide transhydrogenase coding gene pntAB, an aspartic acid transaminase gene aspC, an aspartic acid decarboxylase gene panD derived from pseudomonas aeruginosa and a beta-alanine transporter gene NCgl0580 derived from corynebacterium glutamicum are integrated at the same time, and the bacillus subtilis is used for producing beta-alanine and has the advantage of high fermentation yield.

The invention discloses an application of a soybean GmPT1 coding gene in resisting soybean mosaic virus diseases. The invention discloses application of the soybean inorganic phosphate transporter gene GmPT1 as shown in SEQ ID NO.1 and SEQ ID NO.2 in genetic engineering modification of soybean mosaic virus resistance. The over-expression of the gene can significantly improve the resistance of soybeans to soybean mosaic virus diseases. The soybean inorganic phosphate transporter gene GmPT1 disclosed by the invention can be transformed into soybeans through genetic engineering, and the resistance of the soybeans to soybean mosaic virus diseases is finally regulated and controlled.

Provided are a theanine-producing strain and use thereof in tea fermentation production. A corynebacterium glutamicum is proposed, which includes an alanine decarboxylase CsAlaDC mutant. The theanine-producing strain is obtained by taking the corynebacterium glutamicum as a starting strain, knocking out in sequence an α-ketoglutarate dehydrogenase E1 subunit gene odhA, a glutamate external transporter gene Ncg11221 and a lactate dehydrogenase gene ldh; and / or expressesing a citrate synthase gene gltA, a pyruvate kinase gene pyk and a glutamate dehydrogenase gene gdh; and / or overexpressing an alanine dehydrogenase alaA and integrating a γ-glutamine synthetase GMAS into a cg1960 pseudogene locus of the corynebacterium glutamicum.

The invention belongs to the technical field of microorganism application, and particularly relates to a genetically engineered bacterium for producing L-alanine and application of the genetically engineered bacterium in two-stage fermentation of the L-alanine, and the genetically engineered bacterium takes vibrio natrievibrio as a chassis bacterium and can co-express an alanine dehydrogenase gene and an alanine transporter gene. An alanine dehydrogenase gene is heterologously introduced into a vibrio natriticus genome, and an inactivated gene combination with the optimal L-alanine yield is found out by randomly inactivating a key enzyme in a byproduct production pathway, so that the vibrio natriticus genetically engineered bacterium capable of efficiently producing the L-alanine through a fermentation method is constructed. According to the present invention, the L-alanine is produced through the two-stage fermentation by using the gene engineering bacteria through the temperature-sensitive control strategy so as to reduce the influence of the accumulation of the L-alanine on the cell growth, and the high production of the Xiabanmate is provided. The genetically engineered bacterium is short in growth cycle, glucose can be efficiently utilized to convert the genetically engineered bacterium into L-alanine, the L-alanine is transferred to the outside of cells, separation and purification are simple, cell disruption is not needed, and the yield and conversion rate of the L-alanine can both reach a high level.

The invention discloses a method for constructing a high-yield natamycin engineering strain through overexpression of an extracellular transport protein gene pimA and a pathway specific regulation gene pimR, and the high-yield engineering strain is applied to fermentation production of natamycin. The yield of the natamycin can reach 19.49 g / L through fed-batch fermentation in a fermentation tank of 5L of the high-yield strain S.ilvosporeus pimA-pimR, and the yield of the natamycin is improved by 33.12% compared with the yield of a chassis strain S.ilvosporeus TUST01; the production intensity is improved by 33.33% compared with the production intensity of a chassis strain S.ilvosporeus TUST01, and the strain has a relatively high industrial application value.

The present invention relates to the field of plant genetic engineering, and specifically to the application of an amino acid transporter gene. This solution reduces the expression level of the OsAAP7 gene in wild-type rice or knocks out the OsAAP7 gene in wild-type rice; the amino acid sequence of the OsAAP7 protein encoded by the OsAAP7 gene is shown in SEQ ID NO.1, or the OsAAP7 protein is a protein with equivalent activity obtained by substituting, replacing, and / or adding several amino acids to the amino acid sequence shown in SEQ ID NO.1. This solution reveals the function of the OsAAP7 gene. By knocking out the expression of the OsAAP7 gene, the elongation of the tiller buds of a single rice plant can be accelerated, the number of tillers per rice plant can be increased, and the number and weight of grains per rice plant can be increased. The OsAAP7 gene can be used in rice breeding to increase rice yield.

This invention provides compositions and treatment methods for reducing hyperphenylalaninemia. [Solution] A genetically modified bacterium is provided, comprising: a) one or more genes encoding phenylalanine ammonia lyase (PAL), which are operably linked to a promoter that is not naturally associated with the PAL gene and can be directly or indirectly induced; b) one or more genes encoding a phenylalanine transporter, which are not naturally associated with the phenylalanine transporter gene and can be operably linked to a promoter that is directly or indirectly induceable; and c) one or more genes encoding mutant fumarate and nitrate reductase (FNR), which are not naturally associated with the FNR gene and can be operably linked to a promoter that is directly or indirectly induceable.

The invention discloses a genetically engineered bacterium for synthesizing L-glutamine by taking glucose as a carbon source and application of the genetically engineered bacterium. The genetically engineered bacterium takes escherichia coli as a chassis strain; according to the invention, a citrate synthase gene gltA, an aconitic acid hydratase gene can, an isocitrate dehydrogenase gene icd, a glutamate dehydrogenase gene gdh, a glutamine synthetase gene glnA and ammonium transporter genes amt and amtb can be overexpressed. Compared with the prior art, the invention provides a new strain and a new method for biosynthesizing L-glutamine, and the new strain and the new method have the significances of economy, scientificity, low cost, simple process and large-scale production.

The application discloses an Escherichia coli transmembrane transporter gene and application thereof in vitamin B6 biosynthesis. The application weakens a vitamin B6 transporter gene coding in the Escherichia coli, and the ability of a genetically engineered strain to produce vitamin B6 is greatly improved, and the genetically engineered strain has great application and popularization value. In the best embodiment, the extracellular yield of vitamin B6 of the genetically engineered strain is increased by 33.7% compared with that of a wild type, and the effect is extremely remarkable.

This invention belongs to the field of biological genes and discloses the application of a rice monosaccharide transporter gene OsPLT1 and its transporter in regulating rice growth period. The rice monosaccharide transporter gene... OsPLT1 The nucleotide sequence is shown in SEQ ID NO: 1. This invention also discloses a gene for a rice monosaccharide transporter. OsPLT1 Amplification primers. This invention provides rice. OsPLT1 Novel applications of genes and their encoded proteins in regulating rice growth stage; knockout of wild-type ZH11 using CRISPR / Cas9. OsPLT1 At this time, it can accelerate the growth process of rice without affecting the yield of rice, therefore, OsPLT1 Genes can be used to regulate plant growth periods and increase annual and seasonal yields, and have high economic and application value.

The invention relates to a genetically modified rice plant or plant cell, which comprises a heterologous heavy metal ATP enzyme gene operably linked to an OsActin1 promoter, a heterologous ATP binding cassette (ABC) transporter gene operably linked to the OsActin1 promoter, and a heterologous plant chelating peptide synthase gene operably linked to the OsActin1 promoter, wherein the OsActin1 promoter has a lower activity in the seed endosperm of the modified rice plant compared to the activity in other nutritional tissues of the modified rice plant; wherein arsenic (As) and cadmium (Cd) in rice grains of the genetically modified rice plant are reduced as compared to a control rice plant in which the genetic modification is not performed. The invention also relates to a method for constructing such genetically modified rice plants or plant cells, and a kit for carrying out the method.

This invention belongs to the field of crop breeding, specifically relating to an NtLHT1 gene and its application in tobacco's resistance to low nitrogen stress. It involves artificially intervening in the mutation of specific nucleotide sequence fragments in the NtLHT1 gene to obtain two mutated nucleotide sequence fragments, resulting in a mutant NtLHT1 gene, and the application of the mutant NtLHT1 gene in improving tobacco's resistance to low nitrogen stress. The NtLHT1-edited tobacco plants of this invention exhibit enhanced absorption of nitrate and ammonium nitrogen during their vigorous growth period, increased activity of nitrate reductase and glutamine synthase, increased expression of nitrogen transporter genes, and improved nitrogen utilization efficiency. This invention increases the activity of nitrate reductase and glutamine synthase, thereby improving tobacco's resistance to low nitrogen stress and providing a genetic resource and material basis for the development of nitrogen utilization-related genes and strains in tobacco.

The invention belongs to the technical field of insecticides, and particularly relates to a ds-ABC transporter gene for preventing and controlling resistant Frankliniella occidentalis as well as a screening method and application of the ds-ABC transporter gene. The ABC transport protein gene is selected from any one or more of ABCG20, ABCA1, ABCB8, ABCD2 and ABCA5, and the ABC transport protein gene is selected from any one or more of ABCG20, ABCA1, ABCB8, ABCD2 and ABCA5. The screening method comprises the following steps: (1) testing the resistance level of the Frankliniella occidentalis to spinetoram, imidacloprid and thiamethoxam; (2) extracting and purifying the total RNA of the Frankliniella occidentalis population by using a trace total RNA extraction kit, synthesizing cDNA (complementary deoxyribonucleic acid) by taking the RNA as a template, and then carrying out quantitative primer design and gene expression; and (3) carrying out significant analysis on difference analysis of all data through real-time fluorescent quantitative detection, and screening out 5 ds-ABC transporter genes which are subjected to significant up-regulation expression in a resistant population. According to the invention, the key ABC transporter gene for mediating the resistance of the Frankliniella occidentalis to the spinetoram is screened and determined, and a basis is provided for development of a novel anti-resistance insecticide taking the ABC transporter as a target.

The invention discloses a promoter of an alfalfa malic acid transport protein gene MsALMT1 and application of the promoter. The nucleotide sequence of the promoter is shown as SEQ ID No.1. The invention further discloses a preparation method of the promoter. The invention provides the nucleotide sequence of the alfalfa malic acid transporter gene MsALMT1 promoter, and the alkali resistance of the plant can be obviously improved after the promoter drives the overexpression of MsALMT1 in alfalfa, so that a gene resource is provided for the improvement of the alkali resistance of the plant, and the application value is wide.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a zostera marina magnesium ion transporter gene ZosmaMGT10, a recombinant vector and application. The CDS full length of the gene is 1239 bp, 412 amino acids are encoded, and the gene has a conservative CorA structural domain. It is proved for the first time that ZosmaMGT10 protein has Mg < 2 + > transport activity and can be specifically combined with Mg < 2 + > through E299, E300 and E303 residues, abscisic acid can remarkably induce expression of the ZosmaMGT10 protein in zostera marina leaves, the Mg < 2 + > absorption capacity of zostera marina is enhanced, and then plant stress resistance is improved. The gene ZosmaMGT10 provided by the invention can be used as a key candidate gene, is used for cultivating marine plant varieties with high Mg < 2 + > absorption efficiency and strong stress resistance, and provides a new tool and a theoretical basis for ecological restoration of coastal zones and breeding of stress-tolerant crops.

The application discloses an ABC transporter family gene OsABCA3 and application in plant breeding regulation, belonging to the field of plant genetic engineering. OsABCA3 and application in plant breeding regulation, belonging to the field of plant genetic engineering. OsABCA3 The amino acid sequence of the encoded protein is shown as SEQ ID No: 2. The application realizes the knockout of the ABC transporter gene in rice, OsABCA3 The Cd absorption in the knockout plant under Cd stress is significantly reduced compared with the wild type plant, the Cd content in the grain of the knockout plant planted in Cd contaminated soil is significantly reduced compared with the wild type plant, but the essential element content in the grain and the yield of the rice are not negatively affected, thereby providing a new candidate gene resource for creating safe rice with low Cd content in grain, and providing a potential remediation method for Cd pollution of rice.

The invention discloses an application of a rice phosphorus transporter gene and an encoding protein thereof in improving nutrient utilization efficiency and rice quality. According to the invention, OsPHT1 is mutated or inhibited; 1 gene expression can significantly improve nutrient utilization efficiency and rice taste quality and nutritional quality. The method is mainly embodied in that accumulation of nitrogen and phosphorus nutrients of rice is remarkably reduced, the total protein content, glutelin content, prolamin content, globulin content, albumin content and phytic acid content of rice grains are remarkably reduced, the gel consistency of the rice is increased, the reduction value is reduced, and the eating quality and the nutritional quality of the rice are further improved.

The invention discloses a method for enhancing cholesterol uptake of saccharomyces cerevisiae, and belongs to the technical field of bioengineering. The method comprises the following steps: knocking out transcription inhibition factors MOT3 and ROX1 of genes related to cholesterol uptake and transport of saccharomyces cerevisiae; the transcription activating factors SUT1 and UPC2-1 of the cholesterol transporter gene are over-expressed; expression of sterol esterifying enzyme ARE2 is enhanced, so that uptake and storage of exogenous cholesterol by the saccharomyces cerevisiae are further enhanced. According to the method disclosed by the invention, the uptake and storage of cholesterol by the saccharomyces cerevisiae are enhanced, and an important reference is provided for construction of a steroid hormone synthesis chassis taking cholesterol as a substrate.

The present invention belongs to the field of genetic engineering technology, specifically relating to genetically engineered bacteria and their use in xylitol production. The genetically engineered bacteria include a host cell and a xylose reductase gene and a xylose transporter gene inserted into the host cell. The present invention introduces the xylose transporter gene and the xylose reductase gene into Vibrio natriuresis. The resulting genetically engineered bacteria can functionally express and obtain a recombinant xylose transporter, thereby transporting extracellular xylose into the cell and converting the xylose into xylitol using the xylose reductase. This invention is the first to construct a genetically engineered Vibrio natriuresis bacterium capable of utilizing xylose and producing xylitol, which is of great significance for subsequent research on the use of Vibrio natriuresis in xylitol production.

The invention discloses application of a monosaccharide transporter gene OsSTP28 in improvement of rice plant height and lodging resistance, provides application of a green and friendly rice gene OsSTP28 in improvement of rice plant height and lodging resistance, and discovers that knockout of the rice OsSTP28 gene can reduce rice plant height and increase rice lodging resistance.

The application provides a Chlamydomonas reinhardtii ABC transporter gene CrABCG, a coding protein and application thereof, and the gene comprises CrABCG3 or CrABCG5, and the gene sequence comprises SEQ ID NO:1 or SEQ ID NO:2. It is found by the application that the CrABCG protein is related to carbon and nitrogen metabolism of Chlamydomonas reinhardtii, and preliminary researches show that the expression of the CrABCG3 gene of Chlamydomonas reinhardtii is increased after nitrogen limitation, while the expression of the CrABCG5 gene is first decreased and then increased, and the results show that the CrABCG protein influences the transport, synthesis and accumulation of lipid substances. Therefore, the engineering algae strains with high lipid content can be obtained by screening and modification of the algae strains with high expression of the CrABCG gene.

The invention discloses a construction method and application of escherichia coli genetically engineered bacteria for producing malic acid by synergistically utilizing CO2, and belongs to the technical field of bioengineering. According to the invention, an L-malic acid chassis strain MG1655W (DE3) constructed by a gene editing technology in a laboratory is used as an initial strain, a lactic dehydrogenase encoding gene ldhA and a phosphate acetyltransferase encoding gene pta in escherichia coli are knocked out on the basis, the production paths of lactic acid and formic acid are blocked, and the yield of lactic acid and formic acid is increased. The method comprises the following steps of: firstly, amplifying a phosphoenolpyruvate carboxylase gene ppc and a CO2 transporter gene CA, constructing a recombinant plasmid, introducing the recombinant plasmid pACYCDuet-1-CA-ppc into an expression host MG1655W (DE3) delta ldhA delta pta, and screening to obtain the engineering bacterium MG1655W (DE3) delta ldhA delta pta-CA-ppc by overexpressing the ppc gene and the CA gene and improving the CO2 immobilization and concentration capability of escherichia coli. The obtained engineering bacterium is subjected to cell proliferation under an aerobic condition and malic acid accumulation under an oxygen limiting condition, the final malic acid yield is increased to 140-212%, and the engineering bacterium has important industrial application value.

The invention discloses a nano insecticide and a preparation method and application thereof.The nano insecticide is formed by loading insecticide active ingredients on ZIFs series nano materials, and the ZIFs series nano materials take # imgabs 0 # or # imgabs 1 # as metal ions and are self-assembled with imidazole organic ligands to form a porous crystal structure; according to the nano insecticide, the toxicity of the insecticide is enhanced by inhibiting the expression of ABC transporter genes of pests. The sensitivity of the resistant pests to the insecticide is improved, and wide popularization and application values are realized.

The invention discloses a construction method and application of a snakehead iron overload type liver injury model, and belongs to the technical field of animal models. Comprising the following steps: randomly dividing 700-800 snakeheads into four groups, wherein each group comprises six repetitions; feeding the snakehead with feed containing ferrous sulfate heptahydrate with different gradients; the method comprises the following steps: collecting snakehead serum and / or liver samples, carrying out kit determination, Prussian blue staining and Hamp; e, dyeing, observing through a transmission electron microscope and Tunel dyeing, detecting the expression quantity of liver iron transporter genes and proteins, and detecting cell apoptosis; and determining the optimum dosage of the ferrous sulfate heptahydrate induced snakehead liver injury, and repeating the steps to give the optimum dosage of ferrous sulfate heptahydrate induced snakehead liver injury to obtain the snakehead iron overload type liver injury model. The constructed snakehead iron overload type liver injury model is good in standardization and repeatability, can be used for animal model construction or drug screening for treating iron overload type liver diseases, and provides a theoretical basis for deep research of subsequent fish iron overload type liver injury.

The invention provides an application of a potassium transporter gene OsHAK25 in improving the phosphorus absorption capacity of rice, a nucleotide sequence of the OsHAK25 gene is shown as SEQ ID NO.2, and the OsHAK25 gene negatively regulates the phosphorus absorption capacity of the rice. The OsHAK25 gene of rice is knocked out to obtain an oshak25 mutant plant, under phosphorus treatment, the content of inorganic phosphorus in leaves of the oshak25 mutant plant and a wild type plant is measured, it is found that the content of the inorganic phosphorus in the leaves of the oshak25 mutant plant is remarkably higher than that of the wild type plant, and it is indicated that the phosphorus absorption capacity of the oshak25 mutant plant is higher. By analyzing the expression quantity of the phosphorus absorption related genes of the overground parts and root systems of an oshak25 mutant plant and a wild type plant, it is found that the expression quantity of the OsSPX2 genes of the overground parts and root systems of the oshak25 mutant plant is significantly lower than that of the wild type plant. It is shown that an oshak25 mutant plant can increase the expression quantity of phosphorus absorption related genes such as the OsPT2 gene, the OsPT3 gene and the OsPAP21b gene by down-regulating the expression quantity of the OsSPX2 gene, and therefore phosphorus absorption of rice is promoted.

Provided are a genetically engineered bacterium for producing lacto-N-tetraose, a preparation method therefor, and use thereof. The genetically engineered bacterium overexpresses an exogenous transporter gene BmSet. The genetically engineered bacterium can enable lacto-N-tetraose to be quickly and efficiently excreted out of cells, promote the increase in the fermentation yield of lacto-N-tetraose, and increase the concentration of lacto-N-tetraose in extracellular media, thereby having good industrial application prospects.

The application discloses a strain of Concretic Hendonexis CCFM1541 capable of promoting intestinal mineral absorption and improving intestinal flora, and a postbiotic thereof, and belongs to the technical field of microorganisms for promoting zinc and calcium absorption and improving intestinal flora. The Concretic Hendonexis CCFM1541 in the application can promote the increase of zinc and calcium absorption of mice, and improve intestinal flora, and the specific embodiments are as follows: (1) restoring the organ index, serum zinc and ALP content of mice, and regulating the expression of zinc-related transport genes in the small intestine; (2) improving the serum calcium and ALP content of mice, restoring the length, diameter and weight of femur, and effectively promoting bone formation and optimizing the trabecular microstructure; (5) effectively reversing the decrease of intestinal flora diversity and structural disorder induced by antibiotics, and restoring the intestinal microecological homeostasis. Therefore, the Concretic Hendonexis CCFM1541 has great application prospect in promoting intestinal mineral absorption and regulating intestinal flora.

This invention belongs to the field of fungal genetic engineering technology and discloses the ABC transporter gene of shiitake mushroom. LeABC2 And its application in regulating the heat tolerance of shiitake mushrooms. Genome-wide association analysis was performed based on the heat tolerance phenotypic data of 133 shiitake mushroom strains to identify candidate genes regulating heat tolerance. LeABC2 , build LeABC2 The gene function was studied using an RNA interference vector via Agrobacterium-mediated genetic transformation. The results showed... LeABC2 The gene negatively regulates the heat resistance of shiitake mushroom mycelium, and interfering with the expression of this gene can improve the heat resistance of mycelium, providing a new solution to the problem of high temperature affecting yield and quality in the shiitake mushroom industry.

The invention relates to the technical field of rice molecular breeding, in particular to application of a gamma-aminobutyric acid (GABA) transporter gene OsGAT3 in improving rice yield breeding and a method. After overexpression of the cloned OsGAT3 gene, the yield, salt resistance and drought resistance of the rice are remarkably improved, which shows that the OsGAT3 gene has obvious influence on the yield, salt resistance and drought resistance of the rice, so that the yield, salt resistance and drought resistance of the rice can be genetically improved by improving the expression of the OsGAT3 gene through a genetic engineering technology. Based on the functions of the OsGAT3 gene found by the inventor, the OsGAT3 gene can be used for improving the rice yield and breeding with salt resistance and drought resistance. The purpose of rice salt-resistant and drought-resistant breeding is to cultivate high-yield rice plants with relatively strong salt-resistant and drought-resistant capabilities. Expression of the OsGAT3 gene can be improved through an overexpression technology, and rice plants with high yield, salt resistance and high drought resistance are obtained.