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Kit and method for detecting genes related to individualized medication for depression

A kit, depression technology, applied in the direction of DNA / RNA fragments, recombinant DNA technology, etc., can solve the problems of inaccurate prediction, few drugs and genes, and lack of treatment options for patients with refractory depression, so as to achieve comprehensive and accurate detection , time-saving effect

Pending Publication Date: 2019-08-23
广州海思医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Existing detection products focus on the depression drug itself, and not enough attention is paid to the synergistic treatment of depression, which is a new possible treatment direction for patients with refractory depression; currently on the market for detection products for depression patients There are fewer drugs and genes covered, and the prediction is not accurate enough, especially for patients with treatment-resistant depression, there is a lack of new and potentially effective treatment options

Method used

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  • Kit and method for detecting genes related to individualized medication for depression
  • Kit and method for detecting genes related to individualized medication for depression
  • Kit and method for detecting genes related to individualized medication for depression

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Experimental program
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Effect test

Embodiment 1

[0057] An embodiment of the method for detecting genes related to individualized medication for depression of the present invention is mainly used for library construction and result reporting. Using the library construction method of the present invention, it is possible to simply and quickly construct antidepressant drugs and synergistic The sequencing library of the gene loci of the drug can detect the mutations of 26 related genes at the same time. At the same time, the established depression database system can provide treatment plans including first-line and second-line antidepressants and synergistic drugs based on the results of genetic testing and combined with the specific conditions of patients, providing patients with highly feasible treatment plans, especially for refractory patients. A new and potentially effective solution for patients with sexual depression. The method of the present invention can be used not only on the illumina sequencing platform, but also o...

Embodiment 2

[0088] The preparation of embodiment 2 kit

[0089] An embodiment of a test kit for detecting genes related to depression individualized medication in the present invention, the test kit can detect 26 gene polymorphisms simultaneously, including reaction solution 1, amplification primers (such as SEQ NO.1~SEQ NO.154), reaction solution 2, specific linker and quality control.

[0090] According to the experimental comparison, reaction solution 1 is a multiplex PCR reaction solution purchased from Kapa Biosystems;

[0091]According to experiments, the amplification primers are SEQ NO.1~SEQ NO.154 primers designed in Example 1, which were synthesized by Sangon Bioengineering (Shanghai) Co., Ltd., prepared as a 10 μM working solution with sterilized purified water, mixed with It is a mixed solution with the concentration of each primer at 0.01-0.05 μM;

[0092] According to the experimental comparison, the reaction solution 2 is the amplification PCR reaction solution, which is ...

Embodiment 32

[0106] Example 3 2 cases of blood sample detection

[0107] The present invention can be used to detect various samples such as blood, buccal swab, dried blood film, etc. Now, two cases of anticoagulated blood samples are taken as examples to illustrate the specific use of the kit in Example 2.

[0108] (1) Sample DNA extraction

[0109] Take 1-2ml of anticoagulant blood samples from 2 cases, use the blood genomic DNA extraction kit (spin column type) produced by Tiangen Biochemical Technology (Beijing) Co., Ltd. (article number: DP318 specification: 50 / 200 servings), follow the Operation for DNA extraction.

[0110] The DNA concentration (≥1 ng / μL) and purity (1.6-2.0) of the extracted DNA were determined using an ultra-micro-volume ultraviolet spectrophotometer.

[0111] (2) Library construction

[0112] 1) Target fragment amplification

[0113] Add reagents and sample DNA in order to amplify and enrich the target gene: 112.5 μL of reaction solution, 2 μL of amplificatio...

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Abstract

The invention discloses a primer group, a kit and a detection method for detecting genes related to individualized medication for depression. The primer group is selected from nucleotide sequences inSEQ ID NO.1-SEQ ID NO.154. The detection kit covers eight major categories of clinical first-line and second-line antidepressant efficacy-related genes, and relates to drug metabolism, target spots and transporter gene loci, and detection is conducted comprehensively and accurately. Accordingly, aiming at part of patients suffering from treatment-refractory depression, gene detection related to the therapeutic effect of antidepressant synergistic drugs is added, and powerful support is provided for selection of therapeutic schedules of the treatment-refractory depression.

Description

technical field [0001] The invention relates to the technical field of gene detection, in particular to a kit and method for detecting genes related to individualized medication for depression. Background technique [0002] Depression, also known as depressive disorder, is a common mental illness. According to statistics from the World Health Organization, by 2020, depression will become the second leading disease after heart disease. At present, more than 300 million people in the world are suffering from depression. The prevalence rate of depression in China is 6.1%, which is 3 percentage points higher than the global average, and the incidence rate has been increasing year by year in recent years. It is converted according to the incidence rate of 6.1%. , domestic depression patients have reached 90 million. [0003] At present, the most effective means of treating depression is drug therapy, but it is still in the stage of trial and error, and the efficacy and adverse ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883
Inventor 夏昊强周煌凯刘倩邓美英曹治家李洁莹杜智慧
Owner 广州海思医疗科技有限公司
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