103results about How to "Quick checkout" patented technology

The invention provides a method for screening toxic substances in a sample by using solid phase support liquid-liquid extraction-GC MC. The method comprises the following steps: extracting the sample by using a mixed solution containing a water-soluble organic solvent and deionized water, centrifuging an extracting solution after extraction, collecting a supernatant and removing the organic solvent in the supernatant, and preparing a sample liquid; adding the sample liquid into an activated solid phase support liquid-liquid extraction column, performing standing, performing eluting by using an eluate, and collecting the eluate; carrying out a chromatogram-mass spectrometry of the eluate. According to the invention, different toxic substances of a plurality of structures in a sample can be rapidly and effectively detected, especially screening of more than 150 kinds of toxic substances can be realized by single determination with high detection efficiency; the method provided by the invention is especially suitable for fields such as forensic science and has a good praticical value.

The invention discloses a moving-target detection and tracing system and a method thereof. The system includes a moving-target tracing system, which predicts coordinates of a target, which is contained in a next video image frame, on the basis of an image of the target contained in a current video image frame and coordinates in the current video image frame; and also includes a camera video collection module, which realizes real-time collection of each frame of picture of a camera to use the same as real-time image data input of a moving-target detection module and a moving-target tracking module; the moving-target detection module, which realizes detection and recognition on the moving target, and transmits original coordinate information of the to-be-tracked target to the moving-target tracking module; and the moving-target tracking module, which realizes tracking on the moving target, and sends the real-time coordinates of the tracked object to a control module to realize real-timetracing. Through reasonably selecting algorithms of module realization and reasonably allocating a computation amount on an embedded system including logic hardware and a general processor, highly efficient and accurate real-time tracing is realized, and a power consumption requirement of a mobile device is satisfied.

The invention relates to a primer group for rapid detection of 2019-nCoV based on a CRISPR technology and application thereof, and belongs to the technical field of gene detection of the CRISPR technology. The primer group comprises an Orf1ab gene amplification primer pair, an Orf1ab gene crRNA, an N gene amplification primer pair and an N gene crRNA. The primer group is adopted to detect 2019-nCoV by the CRISPR technology, the detection time of 2019-nCoV is shortened, and detection can be completed within 40-60min. A specific sequence combination is obtained through screening to serve as theprimer group for detection, the primer group has the advantages of being high in sensitivity and specificity, and the detection limit can reach 7.5copies. The primer group is adopted to conduct CRISPRdetection on 2019-nCoV, dependence on complex variable-temperature amplification instruments such as a qPCR instrument is eliminated, and the CRISPR-Cas technology has wide application prospects in the aspect of real-time diagnosis of 2019-nCoV.

The invention provides a kit for detecting enterocytozoonhepatopenaei and a method for detecting whether aquatic foods contain enterocytozoonhepatopenaei. Besides a primer pair, the kit further comprises nucleic acid extracting liquid. The nucleic acid extracting liquid is formed by 4% of sodium dodecyl sulfate (SDS) and 1.5M Tris/HCl (at the pH of 8.8). An established TaqMan real-time real-time fluorescence quantification polymerase chain reaction (PCR) method has a detection reaction sensitivity as high as 5.20*102 copies per microliter. Compared with ordinary PCR, the sensitivity of the method is 10 times higher than that of the conventional PCR.

The invention belongs to the fields of biology and medicines and particularly relates to a method for detecting a resistant mutant of mycoplasma pneumoniae. By adopting Real-time PCR (Polymerase Chain Reaction) and a cycling probe technology for distinguishing the difference of monobases and searching a specific primer aiming at the mycoplasma pneumoniae in an upstream and a downstream sequence of 2063 bit/2064 bit of the mycoplasma pneumoniae 23SrRNA gene, specific cycling probes aiming at mutation-free sensitive strains, 2063-bit mutant drug-resistant strains and 2064-bit mutant drug-resistant strains can be respectively designed according to the difference of the bases at the 2063 bit/2064 bit of the mutation-free sensitive strains and mutant drug-resistant 23SrRNA gene of the mycoplasma pneumoniae; the PCR amplification can be carried out by using the specific primer of the mycoplasma pneumoniae; and the fluorescence intensity change can be read by a Real-time PCR thermal cycler in real time to determine the mutant types of samples to be detected. The invention can correctly distinguish the mutation-free sensitive strains from the mutant drug-resistant strains of clinical separation strains of the mycoplasma pneumoniae. The specificity is 100 percent and the sensitivity can reach 102copy/PCR reaction.

The invention provides a method for screening toxic substances in a sample by using solid phase microextraction-GC-MS. The method comprises the following steps: extracting the sample by using a mixed solution containing a water-soluble organic solvent and deionized water, and producing an extract; centrifuging the extract, collecting a supernatant and removing organic solvents in the supernatant, performing centrifuging again, collecting a supernatant, and producing a sample liquid; inserting a PDMS extraction head into the sample liquid for carrying out solid phase microextraction, after extraction, fetching out the PDMS extraction head, inserting the extraction head into the GC-MS for desorption, and analyzing the desorbed sample which enters into a chromatographic column by using a GC-MS method. According to the invention, different toxic substances of a plurality of structures in a sample can be rapidly and effectively detected, especially screening of more than 60 kinds of toxic substances can be realized by single determination with high detection efficiency; the method provided by the invention is especially suitable for fields such as forensic science and has a good praticical value.

The crank angle sensor (14) herein comprises a first sensor (15) and a second sensor (16) which are disposed along the circumference of the rim of a signal rotor (12), and determines rotating direction of a crankshaft (11) based on relationship of the outputs of the two sensors (15, 16). The crank angle sensor (14) outputs crank angle signals of different pulse widths in accordance to rotation directions to an engine control circuit (18). The engine control circuit (18) measures the time required by a predetermined number of crank angle signals output by the crank angle sensor (14), the time being seen as predetermined crank angle time. The engine control circuit (18) determines the presence or absence of abnormity of the crank angle sensor (14) by comparing whether the current predetermined crank angle time the previous predetermined crank angle time (or the predetermined crank angle time before the previous predetermined crank angle time) fluctuates at the predetermined value or alarge value.

The invention belongs to the technical field of ceramic tile production devices and particularly relates to an automatic package inspection line for ceramic tiles. An inspection device (1) for inspecting defective goods out, a glue spray device (3) for spraying scratch-proof glue layers to the surfaces of the ceramic tiles (5), a feeding platform for sorting the sequence of the ceramic tiles (5) and conveying the ceramic tiles, an input line (4) located at the front end of the feeding platform (6) and in butt joint with the glue spray device (3), a stacking device (7) located at the tail end of the feeding platform (6) and stacking the ceramic tiles (5) according to the sequence of the ceramic tiles (5) and the preset rule and an output line (8) which is used for outputting the stacked ceramic tiles (5) are sequentially arranged according to the flowing direction of the ceramic tiles (5), wherein the head end of the output line is in abut joint with the stacking device (7). The automatic package inspection line for the ceramic tiles is high in automation degree, achieves assembly type production, has the functions of inspection, glue spraying, sorting, stacking and the like and overcomes the various defects existing in the manual ceramic tile package inspection manner.

The invention discloses a novel recombinant Akabane virus nucleotide capsid protein, gene encoding the recombinant protein and the process for preparing the recombinant protein. The recombinant protein has the amino acid sequence represented by SEQ ID No:1, the gene encoding the recombinant Akabane virus nucleotide capsid protein has the amino acid sequence represented by SEQ ID No:2. The preparing process of the recombinant protain comprises the following steps: constructing recombinant Akabane virus nucleotide capsid protein prokaryotic expression vectors, transforming bacillus coli with the recombinant Akabane virus nucleotide capsid protein prokaryotic expression vectors, inducing recombinant nucleotide capsid protein expression with IPTG, reclaiming and purifying the expressed recombinant nucleotide capsid protein.

The invention relates to the technical field of Raman detection, and discloses a preparation method of a high-performance SERS active substrate ZIF-8/Ag-Au/Si-NPA, which is characterized by comprising the following steps: 1) preparing a nano porous silicon columnar array with favorable reducibility; (2) preparing and optimizing an Ag-Au/Si-NPA SERS active substrate; (3) preparing a ZIF-8/Ag-Au/Si-NPA SERS active substrate; (4) testing the Raman enhancement performance of the ZIF-8/Ag-Au/Si-NPA SERS active substrate. According to the preparation method of the high-performance SERS active substrate ZIF-8/Ag-Au/Si-NPA, through the interaction of the pi-pi bonds formed by the ligand and a benzene ring of PCP, H atoms on PCP can form hydrogen-bond interaction with the ligand, and open metal sites and pi electrons on PCP form pi-complexing interaction, so that the selectivity and the adsorbability of the substrate to PCP are jointly improved. The method disclosed by the invention is low in cost, simple and easy to operate, capable of rapidly detecting the PCP in the liquid phase with high sensitivity, and good in application prospect.

The invention provides a magnetic layered double hydroxide-metal organic framework composite material as well as a preparation method and application thereof, belonging to the technical field of composite materials. The structure of the magnetic layered double hydroxide-metal organic framework composite material is Fe3O4@ZnAl-LDH/ZIF-8. The preparation method comprises the following steps: preparing Fe3O4@ZnAl-LDH by adopting a coprecipitation method, and generating ZIF in situ on the surface of Fe3O4@ZnAlLDH by adopting a solvothermal method to prepare the Fe3O4@ZnAl-LDH/ZIF-8 composite material. The magnetic layered double hydroxide metal-organic framework composite material is applied to the field of micro-extraction, so rapid magnetic separation can be achieved, the magnetic layered double hydroxide metal-organic framework composite material can be recycled, extraction time is shortened, and experiment cost is reduced. Meanwhile, through hybrid ZIF-8, the specific surface area of ZnAl-LDH is increased, and the extraction efficiency of ZnAl-LDH on a target compound is greatly improved. The composite material can sensitively, simply, conveniently and quickly detect target components, can meet analysis requirements on EDCs in milk samples, and also shows good application potential in the aspect of trace organic pollutant analysis and determination.

The invention discloses an LAMP (Loop-mediated isothermal amplification) kit for detecting acinetobacter baumannii, special primers of the LAMP kit and an application of the LAMP kit to detection of the acinetobacter baumannii. The nucleotide sequence of the five special primers for performing LAMP detection for the acinetobacter baumannii in a sequence table is SEQ ID NO: 2(F3), SEQ ID NO: 3(B3), SEQ ID NO: 4(FIP), SEQ ID NO: 5(BIP) and SEQ ID NO: 6(LF). The acinetobacter baumannii can be rapidly, conveniently, efficiently, high-specifically and high-sensitively detected at the equal temperature, complicated instruments are omitted, and a novel technical platform for detecting the acinetobacter baumannii is provided. The LAMP kit can be used for screening and detecting the acinetobacter baumannii in livestock production units, basic medical and health units and disease prevention and control centers, has wide market prospects and high economic and social benefits and is suitable for wide-range popularization and application.

The invention belongs to the technical field of immune protein preparation and applications thereof, and particularly relates to a test paper for detecting African swine fever virus antibody. The invention provides a gene sequence E183L-1 for encoding an extracellular region of an African swine fever virus p54 protein. Based on a general inventive concept, the invention also proposes a primer pairfor amplifying the gene sequence and a synthetic protein encoded by the gene sequence. In order to solve the problems existing in the detection of the African swine fever virus, the invention prepares the test paper for detecting the antibody of the African swine fever virus by utilizing the synthetic protein encoded by the gene. The test paper can rapidly and accurately detect the antibody of the African swine fever virus, and is very suitable for basic-level and on-site rapid detection and diagnosis.

The invention discloses a method and device for detecting gene fusion mutation, a storage medium, a processor and a method for standardizing the transcription group data expression quantity so as to reduce the false positivity of detection. The method comprises the following steps of: detecting whether a sample to be detected accords with a known fusion mutation type from a transcript level; if not, detecting whether the sample to be detected has potential fusion, wherein the potential fusion comprises at least one of the following: unknown fusion exists on the transcript level of a protooncogene; the protooncogene has structural fusion at the genome level; if so, detecting whether the potential fusion is sense fusion or not, and if so, detecting whether the expression level of the protooncogene is abnormal in the following two aspects: over-expression of a functional area of the protooncogene; significant differences in the expression quantities of the protooncogene in 5'and 3'ends. If yes, judging to be positive, otherwise judging to be negative. The method integrates multi-dimensional fusion characteristics to reduce false positives of detection results.

The invention relates to a method of general-purpose primer PCR that is an immune catching method for detecting bacteria. Coating is carried out based on idiosyncratic antibody of various bacteria. With it being closed, the coated antibody is added into the bacteria solution at 36-38 deg.c for immune catching. Then heat denaturation releases template, and cracking liquid is extracted as PCR reaction template. Finally, using general-purpose primer of bacteria gene 16S rRNA to carry out PCR. The invention combines specificity of antigen-antibody with PCR, which has powerful rate of increasing production, to build iUPPCR for detecting pathogenic bacteria. The invention can catch idiosyncratic bacteria and increase caught bacteria so as to reach the goal for rapid detecting idiosyncratic bacteria. The invention possesses features of good repetitiveness, high sensibility and better specificity. It can detect almost any bacterium.

The invention belongs to a detection technology of anaphylactogen in food, in particular to a primer/probe group which is used to detect fish anaphylactogen in food. The invention designs a group of specific primer and probe aiming to main fish anaphylactogen protein parvalbumin according to conservative sequence of gene thereof. Anaphylactogen protein of different fish in food can be fast detected in sensitivityand specificity through using the primer and probe and utilizing the real-time fluorescence PCR technology. The primer and probe can be provided together with other reagent in a reagent kit form, which is used to ribonucleotide amplification reaction. And the process is simply operated and has excellent repetitiveness.

A double real time fluorescent PCR detecting method of enterococcus resisting vancomycin includes applying fluorescent double-channel detection, utilizing two detection wavelength being not influenced to each other to carry out detection, using wavelength A detection probe FPVabA to label fluorescent dyestuff intensity and using wavelength B detection probe FPVanB to label fluorescent dyestuff intensity, confirming that it is negative if C1 value is zero or greater than 37 and confirming that it is positive if C1 value is less than 36.

The invention discloses a kit for detecting porcine pseudorabies virus (PRV) and porcine parvovirus (PPV), which comprises a primer pair 1 and a primer pair 2, wherein one primer in the primer pair 1 is the DNA molecule disclosed as sequence 1 in the sequence table, the other primer in the primer pair 1 is the DNA molecule disclosed as sequence 2 in the sequence table, one primer in the primer pair 2 is the DNA molecule disclosed as sequence 3 in the sequence table, and the other primer in the primer pair 2 is the DNA molecule disclosed as sequence 4 in the sequence table. The experiment proves that the kit can be used for quickly, specifically and sensitively detecting PRV and PPV from the specimen, and lays foundation for clinical quick diagnosis and epidemiology research of PRV and PPV.

The invention discloses a wisdom retail store service system which comprises a databank, a cloud platform and a firewall which are connected together through a network, wherein the databank is used for storing information of goods with RFIDs (radio frequency identification device); the information processed by the databank is transmitted to the cloud platform through the network; and the cloud platform feeds back the information filtered by the firewall to a RFID gateway system, a sensor gateway system and an optical identifying system through the network. The wisdom retail store service system comprises an efficient, rapid and convenient retail store management and service system, various defects of an existing retail store can be solved, the requirements of people in modern living can be met, convenience is brought to exclusive shops, stores and customers, the time is saved, and better service is provided.

The invention discloses CPA primers and a detection kit for detection of methicillin-resistant staphylococcus aureus, and a detection method. The CPA primers are designed for two targets femA and mecAand comprise a stripping primer 4s and 5a, a cross-amplification primer 2a1s, and a specific primer 2a and 3a, wherein the primers have the sequences shown in SEQ ID NO.1-SEQ ID NO.10. The detectionmethod comprises the steps: establishing a cross-primer constant temperature amplification reaction system for detecting femA and mecA, carrying out cross-primer constant temperature amplification reaction, observing the color changes of the two reaction systems, and if colors of both the reaction systems turn into green, indicating that a sample to be tested contains methicillin-resistant staphylococcus aureus; otherwise, indicating that the sample to be tested does not contain methicillin-resistant staphylococcus aureus. The detection time of the primers and method is fast, and the detectionresult can be obtained in about 60 min. In addition, the detection sensitivity is high and reaches the level of f g/[mu]L.

The invention discloses an intelligent automatic ultrasonic detection system and method for in-service basin-type insulators. The detection system comprises a detection process library, a self-adaptive data acquisition module and an intelligent data analysis module; the detection process library comprises an insulator component with a typical structure and a common detection process, provides process parameters required to be set for the self-adaptive data acquisition module, and provides geometric structure parameters of the insulators for the intelligent data analysis module; the self-adaptive data acquisition module provides detection data for the intelligent data analysis module; the intelligent data analysis module realizes automatic positioning of defects according to the corresponding relation between the detection geometric structure parameters and the detection data; and the intelligent automatic ultrasonic detection system is used for detecting the internal defects of the in-service basin-type insulators. The invention realizes the accurate and rapid detection and positioning of the defects of air holes, cracks and the like in the basin-type insulators.

The invention discloses primers, kit and method for detecting methicillin-resistant Staphylococcus aureus by PSR (polymerase spiral reaction). The primers provided herein are shown as SEQ ID NO. 1 to4 and suitable for detecting methicillin-resistant Staphylococcus aureus by PSR; specificity, sensitivity and reliability can be detected for methicillin-resistant Staphylococcus aureus by detecting specific target sequences femA and mecA. The invention also provides a kit and method for detecting methicillin-resistant Staphylococcus aureus by PSR based on the primers; the method has the advantages of high sensitivity, good specificity, good operational convenience, high operational speed, accurate and reliable results, low detection cost and suitability to field detection; no special expensive instruments or agents are required; after developing with a fluorescent dye, the results can be judged with eyes; the kit and method are particularly suitable for detection in small- and medium-sized units and field detection and have a promising application prospect.

The invention relates to the field of traditional Chinese medicine content detection, in particular to a method for detecting content of alpha-homonojirimycin in suregade glomerulata medicinal materials. According to the method, alpha-homonojirimycin is taken as a control, acyl chloride derivatization reagents are used for precolumn derivatization of alpha-homonojirimycin, dichloromethane or chloroform is added to accelerate the reaction, an AgilentExtendC18 chromatographic column is used as the chromatographic column, and the mixture of methanol, acetonitrile and water is used as the mobile phase, and separating determination and quantitative analysis of alpha-homonojirimycin is conducted with high performance liquid chromatography. The detection method for the suregade glomerulata medicinal materials is quick, accurate and convenient.

The invention discloses a LAMP kit for VanB gene detection and a primer special for the same. The LAMP primer for VanB gene detection is designed according to a specific conservative target sequence of a VanB gene and consists of four primer bodies including outer primers VanB12-F3 and VanB12-B3 and inner primers VanB12-FIP and VanB12-BJP. The VanB genes in pure bacteria, patients' faeces, blood and other samples can be qualitatively detected under the isothermal condition in a fast, convenient, efficient, high-specificity and high-sensitivity mode without complex apparatuses, and a new technical platform is provided for VanB gene detection.