Double real time fluorescence PCR detecting method for vancomycin enterococcus

Abstract

A double real time fluorescent PCR detecting method of enterococcus resisting vancomycin includes applying fluorescent double-channel detection, utilizing two detection wavelength being not influenced to each other to carry out detection, using wavelength A detection probe FPVabA to label fluorescent dyestuff intensity and using wavelength B detection probe FPVanB to label fluorescent dyestuff intensity, confirming that it is negative if C1 value is zero or greater than 37 and confirming that it is positive if C1 value is less than 36.

Description

technical field [0001] The invention relates to a method for detecting vancomycin-resistant enterococcus by double real-time fluorescent PCR. Background technique [0002] Vancomycin, a glycopeptide antibiotic discovered in the 1950s, was used as the drug of choice for the treatment of multidrug-resistant G+ bacterial infections due to its unique mechanism of action. last line of defense". But with the emergence of Vancomycin Resistant Entorecoccus (VRE), this last line of defense is also in danger of collapsing. Clinically, VRE can not only cause urinary tract infection, abdominal cavity, pelvic infection and surgical wound infection, bacteremia, endocarditis, meningitis, etc., but also transfer its resistance to other pathogenic microorganisms. Therefore, it is not only the VRE itself that poses a serious threat to humans, but also the transfer of their drug-resistant factors will produce some new drug-resistant strains that are difficult to treat. Strengthening VRE det...

AI Technical Summary

Problems solved by technology

At present, the main method of clinical VRE detection is through antibacterial test. This detection method is not only cumbersome, time-consuming, low sensitivity, low repeatability, but also unable to understand the molecular biological mechanism caused by resistance.