Kit for mesenchymal stem cell culture and application thereof

A cell culture, stem cell technology, applied in animal cells, vertebrate cells, bone/connective tissue cells, etc., can solve the problems of long primary culture time and low purity, and achieve fast cell expansion and expansion efficiency. high effect
CN102965335AInactive Publication Date: 2013-03-13北京清美联创干细胞科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
北京清美联创干细胞科技有限公司
Publication Date
2013-03-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a kit for mesenchymal stem cell culture and its application. The kit provided by the invention includes a cell culture solution A and a cell culture solution B. The cell culture solution A is a Knockout-DMEM culture medium containing 80-120ng / ml cytokine LIF and 80-120ng / ml cytokine bFGF. The cell culture solution B is fetal bovine serum. The kit provided by the invention can separate umbilical cord mesenchymal stem cells from an in-vitro umbilical cord and culture the umbilical cord mesenchymal stem cells, and has the advantages of rapid cell expansion speed and high expansion efficiency. After multiple subculturing, cell totipotency can still be maintained. In the invention, the problems of umbilical cord mesenchymal stem cell separation purity, quantity and primary culture time are solved, and a lot of umbilical cord mesenchymal stem cells can be obtained. Thus, the kit provided in the invention provides abundant sources for making use of umbilical cord mesenchymal stem cells to conduct further experimental research.
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Description

technical field

[0001] The invention relates to a kit for culturing mesenchymal stem cells and its application. Background technique

[0002] Mesenchymal stem cells (MSCs) are important members of the stem cell family, derived from the mesoderm and ectoderm in the early stages of development. Originally found in bone marrow, MSCs have attracted increasing attention because of their multi-lineage differentiation potential, hematopoietic support and promotion of stem cell implantation, immune regulation and self-replication. For example, mesenchymal stem cells can be differentiated into fat, bone, cartilage, muscle, tendon, ligament, nerve, liver, cardiac muscle, endothelial and other tissue cells under specific induction conditions in vivo or in vitro, after continuous subculture and cryopreservation It still has multi-directional differentiation potential and can be used as an ideal seed cell for the repair of tissue and organ damage caused by aging and disease. Mesenchyma...

Claims

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