44 results about "Contact inhibition" patented technology

The invention provides methods for determining whether a subject is suffering from a rheumatoid arthritis associated with the BRAF oncogene comprising contacting isolated fibroblasts from the subject with a molecule or pool of molecules directed to the BRAF oncogene; and culturing the sample in the presence of the agent and determining whether BRAF oncogene expression by the cell is decreased and / or whether cells in the sample return to a less transformed phenotype, exhibit decreased cell proliferation and / or exhibit increased contact inhibition, any of which is indicative that the subject is suffering from a rheumatoid arthritis associated with the BRAF oncogene.

A bioartificial renal tubule is provided that forms an artificial kidney together with a bioartificial glomerulus suitable for continuous hemofiltration. The bioartificial renal tubule includes an artificial membrane having an inner surface coated with renal tubular epithelial cells and a vessel containing the artificial membrane. The cells are prevented by the use of a MEK inhibitor from being stratified and therefore form a confluent monolayer on the artificial membrane. The renal tubular epithelial cells are characterized in that the contact inhibition thereof is maintained by the use of the MEK inhibitor. The MEK inhibitor is preferably U0126. The attachment of cells capable of reproducing the function of a kidney allows dialysis to be continuously performed for 24 hours with high efficiency and also allows the ability of a renal tubule to reabsorb useful substances to be achieved.

The embodiment of the invention provides a cell proliferation system for improving the cell inoculation dispersity and relates to the technical field of cell culture. The cell proliferation system can enable cells to be uniformly distributed and prevent the cells from generating a contact inhibition phenomenon. The cell proliferation system for improving the cell inoculation dispersity comprises a nutrient solution supplying device and an incubator; a cell reactor and a drive motor are arranged in the incubator; the nutrient solution supplying device is communicated with the cell reactor to convey a nutrient solution into the cell reactor; the drive motor is fixedly connected with the incubator; the cell reactor is in transmission connection with the drive motor; and the drive motor can drive the cell reactor to rotate. The cell proliferation system is used for cell culture.

The invention discloses a cell line for lowly-metastatic clear cell carcinoma of kidney of Han Chinese, namely LoMeT-ccRcc CCTCC No.C200910. The cell line can grow for a long time in vitro and be stably handed down, has epithelioid cell form, and has no contact inhibition. The cell is heteroploid, chromosome number and structure are aberrant. Immunohistochemistry shows masculine CA9 and CD133. A flow cytometry analyses and finds out that CA9 and CD133 expression is obviously enhanced in the condition that cell is handed down for many times. The cell line has lowly-metastatic trend after the cell is handed down continuously. The recovery rate of the cell line after being frozen is more than 80%, the recovered cell growth state accords with the original cell. The cell line of the invention is used for establishing a cell model for sieving and preparing medicines that is used for dialogising, preventing and treating clear cell carcinoma of kidney.

The invention provides a triploid loach fin cell line TRMF and an establishing method of the triploid loach fin cell line TRMF. The triploid loach fin cell line TRMF is preserved in the China Typical Culture Collection Center on August 19, 2013 with the preservation number C2013110. The triploid loach fin cell line TRMF is susceptive to a virus of spring viraemia of a carp. The biological characteristics of the triploid loach fin cell line TRMF comprise that cells are like fibroblasts, cytoplasm is rich and grows attaching to cell walls, the cells have the contact inhibition growth characteristic, the cell doubling time is 36.01 hours, and the number of feature chromosomes is 75.

The invention discloses a preparation method of an immortalized human intervertebral disc nucleus pulposus cell system. The preparation method is as follows: extrinsic human telomerase reverse transcriptase is transfected into the human nucleus pulposus cell to activate telomerase and construct immortalized human nucleus pulposus cell line through induction immortalized human nucleus pulposus cell line, normal cells not transformed cells are used, wherein the cells have normal growth rate and karyotype, have contact inhibition, anchorage dependence and other safety properties and do not have carcinogenicity and soft agar cloning capability; and as same as the germline stem cells, the cells have real immortalization, thus the standard cell line can be provided for the further research of the pathogenic mechanism of the degenerative disc disease and a new idea is provided to increase the entire control level of the degenerative disc disease.

The invention relates to a cell line of precursor of forebrain nerve of a telomerase immortalization human embryo and an establishing method and application thereof; the cell line is preserved in China culture collection and management committee general biology center in 21st February 2008, with the collection number of CGMCC No.2372. The cell line of the invention is named as hSN12W-TERT; the cell line expresses growth factor receptor of human nerves and green fluorescent protein, has high activity of telomerase, double core type than normal human, continuous passage culture in vitro of 36 months with 45th generation at the present and population doubling of 100 to 200 times and has the property of contact inhibition and density inhibition; the neuron which is formed by differentiation of the cell line has the property of electrophysiology; the cell line can be differentiated into Gamma-aminobutyric acid (GABA) cholinergic neuron under the induction of bone marphogenic protein 2(BMP2).

Provided are methods and compositions for treating an individual with cancer by administering to the individual a composition that includes a Dectin-2 stimulating agent that stimulates Dectin-2 signaling in myeloid cells (e.g., induces Dectin-2 clustering on the cell surface), thereby stimulating an anti-cancer immune response in the individual. In some cases, the myeloid cells are tumor-associated myeloid (TAM) cells. Methods and compositions are also provided for: treating an individual with cancer via contacting a cancer cell from the individual with an alpha-mannosidase class 1 inhibitor (e.g., to increase the display and / or density of terminal mannose / mannobiose residues on the surface of target cells) in vitro or ex vivo and introducing the contacted cancer cell into the individual; stimulating an antigen presenting cell (APC) via contacting a cancer cell with an alpha-mannosidase class 1 inhibitor and contacting the APC with the inhibitor-contacted cancer cell; and stimulating an APC via contacting it with a subject Dectin-2 stimulating agent.

The invention provides a tetraploid loach fin cell line TEMF and an establishment method thereof. The cell line is collected in the China Center for Type Culture Collection (CCTCC) on August 19th, 2013, and the collection number is CCTCC No:C2013111. The tetraploid loach fin cell line TEMF supports the growth and replication of spring viraemia of carp virus. The biological characteristics of the tetraploid loach fin cell line TEMF include fibroblast-like cell, rich cytoplasm and adherent growth and contact inhibition growth; the cell doubling time is 41.45 hours; the number of characteristic chromosomes is 100.

The invention discloses a device for detecting the density-dependent growth inhibition of cells. The device comprises a culture vessel and 6-10 transparent plastic building blocks; the building blocks are shaped like fans, can be assembled together to form a cylinder and are fixed in the center area of the culture vessel; the area in the culture vessel, except the area occupied by the cylinder, serves as a cell culture observation area; 1 / 2 of the fan-like building blocks for forming the cylinder can be released from fixation, and 1 / 2 of the fan-like building blocks are provided with inclined surfaces which extend to the bottom surface of the culture vessel from the outer edges of the top surfaces of the fan-like building blocks; the outer edge of the top surface of each fan-like building block keeps a distance of 0.5-1.5mm with the bottom surface of the culture vessel, and included angles of 15-42 degrees are formed between the inclined surfaces and the bottom surface of the culture vessel. The invention further discloses application of the device to the dynamic observation of growth and contact inhibition of cells to be detected and the identification of normal cells and malignant tumour cells. The device is a tool provided for experiment teaching to detect the contact inhibition of cells intuitively, conveniently, flexibly and accurately.

The present invention discloses a cell "pungent edge inhibition effect" and contact inhibition viewing dish and its application method. Said viewing dish is formed from culture dish and a cell culture viewing plate which possesses a recess in the centre of its bottom disk and is placed in the culture dish, a perfusion head vertically-positioned in the recess, fixing disk of perfusion head and a fixing steel holder. The application of said device can accurately can visually make observation of cell "pungent edge inhibition effect" and detection of cell contact inhbition. Said device is unique in structure, can utilize microscope to make immediate tracking observation of number of cells, form and position.

The invention relates to an adult human hepatogenic stem cell line HN, a preparation method thereof and an application. The human hepatogenic stem cell line is collected at the China General Microbiological Culture Collection Center, the collection address is No. 3, first yard, Beichen West Road, Chaoyang District, Beijing, the collection date is May 15, 2017, and the collection number is CGMCC No. 13844. The human hepatogenic stem cell line is used for in vitro and vivo directional induction of liver cells and hepatic duct epithelial cells, preparation of biological artificial livers, resistance of acute hepatic failure, chronic hepatic failure and alcoholic fatty livers, inhibition of liver fibrosis and anti-tumor effects. The cells have the biological characteristics that the cells have typical epithelial cell morphology and strong multiplication capacity, grow to achieve contact inhibition and density inhibition, bidirectionally express liver cell specific marker albumin, cytokeratin 8 and biliary epithelial cell specific marker cytokeratin 7 and 9, have various functions of the liver cells, have multi-directional differentiation potential and avoid tumorigenicity.

The invention belongs to the field of microbial animal cell lines, and in particular relates to a human placental site trophoblastic tumor cell line and a constructing method of the human placental site trophoblastic tumor cell line. Through the in-vitro primary culture on the focus in the uterus of patients suffering from placental site trophoblastic tumor, the human placental site trophoblastic tumor cell line is established; the human placental site trophoblastic tumor cell line is named PSTT-1, and is assigned with the accession number of NO.10882. The human placental site trophoblastic tumor cell line has the biological characteristics that the cells grow in single layer in a wall-attaching manner, the contact inhibition is eliminated, the state is nonuniform, and the cells are in the star-shaped or fusiform-shaped flat structure with multiple bulges; the growth in in-vitro culture is good, and the stable and continuous passage for more than 30 generations can be realized; the karyotype analysis result is the normal female karyotype: 46, XX. The PSTT-1 cell line can be used for the research on the nosetiology, the transfer mechanism, the drug-resistant mechanism and the like of the placental site trophoblastic tumor diseases.

The invention discloses a subculture method of a tamarix chinensis tissue culture seedling. In the culture process, quinone type substances released by a plant body can be adsorbed by activated carbon, and contact inhibition growth happens among cells when subculture is conducted on subculture tissue cells and the subculture tissue cells divide; meanwhile, due to the insufficient nutrients and metabolite accumulation in a subculture medium, a cell culture medium needs to be replaced regularly, the quinone type substances released by the plant body during culture can be poured together, and thecultured seedling is prevented from browning.

The invention relates to a semisolid culturing medium. The semisolid culturing medium comprises a methyl cellulose solution, a 2*1640 culturing medium, an L-glutamine solution, a beta-mercaptoethanol solution, fetal bovine serum, a penicillin and streptomycin mixed solution, an HAT solution and an antigen-coated nano-gold solution. The semisolid culturing medium can be directly used for culturing and screening hybridoma cells, can reduce the total subcloning frequency of a culturing process to greatly reduce the labor intensity, and also can make the growth spaces of single cell lines independent to reduce the contact inhibition of clones; and the introduction of antigen-coated nano-gold particles realizes one-step of screening of monoclonal cell lines secreting target antibodies in order to save the monoclonal screening time. The invention also relates to a preparation method of the semisolid culturing medium, and a hybridoma cell screening method using the semisolid culturing medium.

The invention relates to a semisolid culturing medium. The semisolid culturing medium comprises a methyl cellulose solution, a 2*1640 culturing medium, an L-glutamine solution, a beta-mercaptoethanol solution, fetal bovine serum, a penicillin and streptomycin mixed solution, an HAT solution and latex antigen particles. The semisolid culturing medium can be directly used for culturing and screening hybridoma cells, can reduce the total subcloning frequency of a culturing process to greatly reduce the labor intensity, and also can make the growth spaces of single cell lines independent to reduce the contact inhibition of clones. The invention also relates to a preparation method of the semisolid culturing medium, and a hybridoma cell screening method using the semisolid culturing medium.

A method of reducing or inhibiting migration of HIV-infected CD4+ T cells to tissues comprises contacting the infected cells with a ligand that prevents or inhibits the interaction between a T cell-surface fucosylated glycan and its glycan-binding protein or with a molecule that metabolically inhibits glycosylation or fucosylation in said cell. In vitro and in vivo methods are described. Also described is a diagnostic method employing labeled ligands that bind a fucosylated glycan and provide a signal detectable by non-invasive imaging.

The invention discloses a device for detecting the density-dependent growth inhibition of cells. The device comprises a culture vessel and 6-10 transparent plastic building blocks; the building blocks are shaped like fans, can be assembled together to form a cylinder and are fixed in the center area of the culture vessel; the area in the culture vessel, except the area occupied by the cylinder, serves as a cell culture observation area; 1 / 2 of the fan-like building blocks for forming the cylinder can be released from fixation, and 1 / 2 of the fan-like building blocks are provided with inclined surfaces which extend to the bottom surface of the culture vessel from the outer edges of the top surfaces of the fan-like building blocks; the outer edge of the top surface of each fan-like building block keeps a distance of 0.5-1.5mm with the bottom surface of the culture vessel, and included angles of 15-42 degrees are formed between the inclined surfaces and the bottom surface of the culture vessel. The invention further discloses application of the device to the dynamic observation of growth and contact inhibition of cells to be detected and the identification of normal cells and malignant tumour cells. The device is a tool provided for experiment teaching to detect the contact inhibition of cells intuitively, conveniently, flexibly and accurately.

Provided are methods for the design, preparation, and use of non-native pri-microRNAs (pri-miRNAs), pri-microRNA (pri-miRNA) scaffolds, and libraries of non-native pri-miRNAs employing pri-miRNA scaffolds. Also provided are methods for identifying non-native pri-miRNAs, combinations of two or more non-native pri-miRNAs, and miRNAs derived from the processing of such non-native pri-miRNAs, which miRNAs exhibit one or more desired functional activities. Further provided are non-native pri-miRNAs, non-native pri-miRNA libraries, vectors comprising and for the expression of one or more non-native pre-miRNAs or for the expression of one or more miRNAs derived from the processing of one or more pre-miRNAs, and cells comprising one or more non-native pri-miRNAs or one or more miRNAs derived from the processing of such non-native pri-miRNAs, each of which pri-miRNAs, pri-miRNA libraries, vectors, and cells can be prepared by the methods disclosed herein. Still further provided are (a) methods for regulating, promoting, normalizing, restoring, inhibiting, or modulating a desired cellular phenotype including, for example, differentiation, de-differentiation, proliferation, growth, cell death, contact inhibition by expressing one or more pri-miRNAs or one or more miRNAs identified through the screening of a pri-miRNA library according to the methodology disclosed herein and (b) methods for the treatment of a disease or condition that associated with the expression of one or more gene or the production of one or more protein, wherein one or more aspect of the disease or condition is reduced in severity following the expression of one or more pri-miRNAs or miRNAs identified through the screening of a pri-miRNA library according to the methodology disclosed herein.

The present disclosure relates to a method for inhibiting a binding of a SARS-CoV-2 spike protein of a SARS-CoV-2 to an angiotensin-converting enzyme 2 including contacting the SARS-CoV-2 with a sufficient concentration of disulfiram, a method for inhibiting an activity of a main protease (Mpro) of SARS-CoV-2 including contacting a SARS-CoV-2 with a sufficient concentration of disulfiram, and a method for inhibiting an activity of a papain-like protease (PLpro) of SARS-CoV-2 including contacting a SARS-CoV-2 with a sufficient concentration of disulfiram. A method for inhibiting a replication or an infection of a SARS-CoV-2 in a cell includes contacting the cell with a sufficient concentration of disulfiram and a medical composition for use in a treatment of an infection of SARS-CoV-2 including a therapeutically effective amount of disulfiram are also provided.