16 results about "Artificial livers" patented technology

The invention discloses an artificial liver treatment filter pipeline adjustable fixing device which comprises a connecting catheter installed on the side face of a liver treatment filter body, and the connecting catheter is detachably installed on the side face of the liver treatment filter body. A catheter fixing assembly used for fixing the connecting catheter is installed at the position, corresponding to the connecting catheter, of the side face of the liver treatment filter body. During installation, the guide pipe connector is inserted into the connector, the sliding clamping rod is automatically clamped into the clamping groove under the elastic force of the spring, connection is stable, and loosening and falling are avoided. In use, the catheter supporting seat supports the bottom of the catheter, and the stability is enhanced; the telescopic arc-shaped auxiliary support is bent by being attached to the guide pipe, bending is prevented, and liquid circulation and the service life of a pipeline are guaranteed. And the support span can be flexibly changed by adjusting the positioning bolts, catheters and scenes of different specifications can be adapted, the universality and the practicability are improved, reliable support is provided for artificial liver treatment, and the clinical application value is high.

The invention discloses an induction culture medium with definite components and application of the induction culture medium in preparation of a hepatic fibrosis treatment medium, and belongs to the technical field of hepatic cell culture and artificial liver. The induction culture medium is composed of a serum-free basic culture medium, primary bile acid and secondary bile acid, the serum-free basic culture medium is composed of a William's E culture medium, penicillin, streptomycin, L-glutamine, insulin, transferrin, sodium selenite and dexamethasone, the primary bile acid is an FXR signal channel stimulant, and the secondary bile acid is a TGR signal channel stimulant. The hepatic fibrosis treatment medium prepared by utilizing the induction culture medium has a remarkable hepatic fibrosis relieving effect, and has the advantages of definite production materials, controllable process, high product stability and the like.

The invention provides an artificial liver support system. The artificial liver support system comprises a pig liver subjected to gene editing, a patient perfusion module and a pig liver perfusion module. Wherein the patient perfusion module is used for collecting human body blood from the body of a patient, separating plasma, combining the purified plasma into human body blood and inputting the human body blood into the body of the patient. And the pork liver perfusion module is communicated with the patient perfusion module and the pork liver. The pork liver perfusion module is used for receiving the plasma separated by the patient perfusion module, purifying the plasma by adopting the pork liver and conveying the purified plasma to the patient perfusion module. The price of the artificial liver can be reduced, and the treatment effect can be optimized.

This invention discloses a biomimetic decellularized matrix hydrogel and its applications. It relates to the field of biomaterials technology. It comprises the following components: decellularized liver matrix, gelatin, and sodium alginate, and its applications are provided. The therapeutic artificial liver provided by this invention can be mass-produced in vitro using 3D bioprinting technology, exhibiting mature liver functional phenotypes such as ICG uptake / release and drug metabolism in vitro. It was transplanted into a type I tyrosinemia model of liver failure (Fah). ‑ / ‑ It can alleviate liver damage in mice and has the potential for artificial liver transplantation.

A bioartificial liver support system for continuous and discontinuous procedures to provide liver support to a patient, comprising: a chamber containing biomass; a tubular system having multiple circuits adapted to provide independent circuit operation to an apheresis machine or patient; and a monitoring device having one or more sensors adapted to collect, record, analyze, download, and / or store data from the system.

The invention relates to an artificial liver and pancreas tissue in-vitro perfusion culture and monitoring integrated device, a pulsation perfusion system comprises a pulsation perfusion device, a gas input control assembly and a gas supply and exhaust assembly, and the pulsation perfusion device comprises an air bag, a pulsation generation chamber, a gas exchange chamber and a gas mixing chamber. The gas mixing chamber is connected with a gas input control assembly, a gas exchange membrane allowing gas in the gas mixing chamber to pass through is arranged in the gas exchange chamber, the gas bag is controlled to expand and shrink through a gas supply and exhaust assembly, and a left culture bin, an artificial liver pancreas tissue and a right culture bin which are sequentially communicated are arranged in the constant-temperature culture module; one side of the pulsation generation chamber is communicated with a culture medium bottle in the culture medium module through a culture medium liquid inlet pipe, the other side of the pulsation generation chamber is communicated with the left culture bin through a culture medium conveying pipe, and the right culture bin is communicated with a waste liquid bottle in the culture medium module through a culture medium liquid discharge pipe. According to the invention, long-term stable in-vitro perfusion culture of the artificial liver and pancreas tissue and real-time monitoring of the culture state are realized.

The application provides a control method, system, equipment, medium and program product of an artificial liver blood purification pipeline, and relates to the medical field.The method comprises the following steps: acquiring an instruction of turning on or off at least one component in the pipeline; and changing the flow direction of the plasma in the pipeline component according to the instruction.The method automatically controls the conduction and / or disconnection among the pipelines according to different types of treatment equipment, so as to control the flow direction of the blood and the plasma in the pipeline, realize the effect of automatically adapting to various treatment modes, overcome the defect that the currently used pipeline can only complete a single corresponding treatment mode, and comprehensively control the pipeline in an automatic mode, so that the steps of assembling multiple sets of pipelines are avoided, the probability of errors and pollution in the pipeline installation process is reduced, the medical cost is reduced, and the workload of medical staff is reduced.

Provided herein are methods and compositions for obtaining hepatocytes. Provided herein is a kit or cell culture medium composition for inducing hepatocytes from pluripotent stem cells, the composition comprising the following inducers: (1) Activin A, (2) a modulator of the BMP signaling pathway, (3) a modulator of the FGF signaling pathway, (4) a modulator of the Wnt signaling pathway, (5) a growth factor, (6) a TGFβ receptor / ALK5 inhibitor. Also provided herein are methods for preparing hepatocytes by the kit or cell culture medium composition, hepatocytes obtained by the methods, artificial liver devices containing the hepatocytes and / or use of the hepatocytes for preparing artificial liver devices, and methods for large scale culture of hepatocytes.

The flushing connector comprises a tubular connector body, a one-way valve is fixedly arranged at the bottom of the tubular connector body, a lantern ring is fixedly arranged in the middle of the tubular connector body, a universal shaping hose is fixedly arranged outside the lantern ring, and a clamp is fixedly arranged at the other end of the universal shaping hose. A fixing ring is arranged at the upper end of the tubular connector in a threaded connection mode, a clamping mechanism used for clamping and fixing an instillation hose is arranged between the upper end of the tubular connector and the inner wall of the fixing ring in a matched mode, and an ultraviolet sterilization mechanism is fixedly arranged at the bottom of the tubular connector. Clamping and releasing of the instillation hose are realized, so that the flushing joint is quickly connected or disconnected; the flushing connector is fixed on the infusion support through the universal shaping hose and the clamp, and the connector is effectively prevented from shaking or making contact with the inner wall of a waste liquid barrel in the flushing process; the liquid outlet is irradiated by the ultraviolet sterilization mechanism, so that possible bacteria can be effectively killed.

The present invention relates to a method for producing a highly functional artificial organ using aptamers, comprising blood vessels capable of transporting perfusate. When used as a coating agent for a decellularized scaffold, the aptamer according to the present invention enhances vascular adhesion, activity, and angiogenic potential, thereby enabling more effective vascular reconstruction than existing antibodies. Consequently, a vascularized artificial liver prepared using this aptamer reduces thrombosis in vivo and enhances liver function, and is therefore expected to be applicable to methods for treating diseases using artificial organs prepared using the aptamers according to the present invention.

The present invention discloses an artificial liver system teaching device, relating to the field of medical operation training devices. The device comprises an operation panel, a blood purification host system, a blood purification pipeline, and a liquid tank. The blood purification host system is mounted on the operation panel and includes a plasma separator holder and two toner input devices, with the plasma separator holder located in front of the operation panel and the toner input devices located behind the operation panel. The blood purification pipeline is mounted on the blood purification host system and includes a simulated plasma separator, with a liquid inlet cavity provided at the upper end of the simulated plasma separator, and a plasma cavity and a non-plasma cavity provided below the liquid inlet cavity of the simulated plasma separator, each of which is connected to the liquid inlet cavity. The upper end of the plasma cavity is connected to one toner input device, and the lower portion of the non-plasma cavity is connected to another toner input device. Both ends of the blood purification pipeline are connected to the liquid tank.

The invention discloses a preparation method of a hyper-crosslinked macroporous adsorption resin capable of simultaneously adsorbing bilirubin, bile acid and cytokines in blood purification. The preparation method comprises the following steps: preparing macroporous styrene-divinylbenzene resin with low crosslinking degree from a styrene monomer and a polyethylene phenyl crosslinking agent; then carrying out secondary crosslinking on macroporous styrene-divinylbenzene resin with low crosslinking degree by utilizing a Friedel-Grafts method to prepare hypercrosslinked adsorption resin, and further reacting residual chloromethyl with an amination reagent to improve the adsorption performance of bilirubin and bile acid. The prepared adsorbent can adsorb bilirubin, bile acid and cell factors at the same time, the adsorption rate of the adsorbent to bilirubin is superior to that of an anion resin bilirubin perfusion device used clinically, in combined artificial liver treatment, one perfusion device can replace two perfusion devices, the treatment cost and the operation risk are greatly reduced, and the treatment effect is good. Meanwhile, the biocompatibility is superior to that of anion resin, and the good market application value is achieved.

The utility model discloses a jugular vein artificial hepatic duct fixing device which comprises a bottom plate and extension cloth, an adhesive layer is arranged below the bottom plate, and a fixing belt with a plurality of adjusting holes and fixing pieces capable of being clamped into the adjusting holes and fixed are arranged above the bottom plate. The extension cloth is located on one side of the bottom plate, a wrapping bag is arranged above the extension cloth, and adhesive tapes are arranged on the two sides of the extension cloth. By means of the fixing device, the risks of infection and the like caused by edge curling of the sterile application due to sagging of the double-cavity catheter can be reduced. And a plurality of adjusting holes are formed to adapt to catheter heads with different thicknesses, so that the application range is wide, and the fixation is proper. By arranging the extension cloth and the wrapping bag, the extension tube can be wrapped, the situation that the gauze is frequently replaced due to looseness is avoided, the workload of medical staff can be reduced, and meanwhile the discomfort of a patient can be relieved. The device is not only suitable for treatment of non-biological artificial liver, but also suitable for treatment occasions related to blood purification such as kidney dialysis and the like.

A method of obtaining 3D cell structures including differentiated human hepatic cells. The method includes: a first step of culturing stem cell-derived or immortalized human hepatic progenitors in a non-adherent culture vessel, preferably a low or ultra-low attachment culture vessel; a second step of transferring the stem cell-derived or immortalized human hepatic progenitors to a culture medium including methacrylated gelatin (GelMa), thereby embedding the stem cell-derived or immortalized human hepatic progenitors in a GelMa matrix; and a third step of covering the GelMa matrix with culture medium and culturing the stem cell-derived or immortalized human hepatic progenitors embedded in the GelMa matrix, thereby obtaining 3D cell structures including differentiated human hepatic cells. Also, methods for engineering an artificial liver model or an artificial liver organ, and for assessing in vitro the metabolism, toxicity and / or therapeutic effects of a compound.

The invention discloses an artificial liver anticoagulant dynamic regulation and control system and method based on multi-modal monitoring, and relates to the field of anticoagulant use regulation and control. Real-time indexes and personalized treatment schemes of patients can be accurately matched through an anticoagulant type mapping model and an injection rate mapping model which are established based on historical treatment data; by monitoring the residual amount of the anticoagulant in real time and predicting the total demand quantity, advanced early warning is realized in combination with purchase time consumption evaluation, and treatment continuity is ensured; closed-loop feedback is formed through full-process iteration when the type is changed or rate curve reestimation when the type is not changed, and injection parameters are continuously adjusted; the manual intervention frequency is remarkably reduced, dynamic calibration of the dosage of the anticoagulant is achieved through multi-time-point data modeling, and resource waste and the blood coagulation risk are effectively avoided while the treatment effect is guaranteed.

The utility model discloses a multi-purpose multi-head clip for combined artificial liver treatment, which comprises a clip body integrally formed by elastic plastics, a first clip head, a second clip head and a third clip head are annularly arranged on the outer side of the clip body at equal intervals, and an infusion line restraining device is arranged at the upper end of the clip body. And one end of the clamp body is connected with a mounting and fixing structure. According to the design, the three chucks are arranged, the three chucks are all made of elastic plastic, the plasma separator, the plasma adsorber and the perfusion device can be conveniently clamped, the front of an artificial liver machine is clean and tidy, protection pads are arranged in the chucks, the effect of protecting the three devices is achieved, and the three chucks have the anti-skid performance and are not prone to falling off. The infusion line restraining device is arranged on the clamp, infusion tubes connected to the plasma separator, the plasma adsorber and the perfusion device can be restrained, site chaos is avoided, and a plurality of clamps do not need to be arranged on an artificial liver machine.