8 results about "Artificial livers" patented technology

The invention discloses an artificial liver treatment filter pipeline adjustable fixing device which comprises a connecting catheter installed on the side face of a liver treatment filter body, and the connecting catheter is detachably installed on the side face of the liver treatment filter body. A catheter fixing assembly used for fixing the connecting catheter is installed at the position, corresponding to the connecting catheter, of the side face of the liver treatment filter body. During installation, the guide pipe connector is inserted into the connector, the sliding clamping rod is automatically clamped into the clamping groove under the elastic force of the spring, connection is stable, and loosening and falling are avoided. In use, the catheter supporting seat supports the bottom of the catheter, and the stability is enhanced; the telescopic arc-shaped auxiliary support is bent by being attached to the guide pipe, bending is prevented, and liquid circulation and the service life of a pipeline are guaranteed. And the support span can be flexibly changed by adjusting the positioning bolts, catheters and scenes of different specifications can be adapted, the universality and the practicability are improved, reliable support is provided for artificial liver treatment, and the clinical application value is high.

A bioartificial liver support system for continuous and discontinuous procedures to provide liver support to a patient, comprising: a chamber containing biomass; a tubular system having multiple circuits adapted to provide independent circuit operation to an apheresis machine or patient; and a monitoring device having one or more sensors adapted to collect, record, analyze, download, and / or store data from the system.

Provided herein are methods and compositions for obtaining hepatocytes. Provided herein is a kit or cell culture medium composition for inducing hepatocytes from pluripotent stem cells, the composition comprising the following inducers: (1) Activin A, (2) a modulator of the BMP signaling pathway, (3) a modulator of the FGF signaling pathway, (4) a modulator of the Wnt signaling pathway, (5) a growth factor, (6) a TGFβ receptor / ALK5 inhibitor. Also provided herein are methods for preparing hepatocytes by the kit or cell culture medium composition, hepatocytes obtained by the methods, artificial liver devices containing the hepatocytes and / or use of the hepatocytes for preparing artificial liver devices, and methods for large scale culture of hepatocytes.

The flushing connector comprises a tubular connector body, a one-way valve is fixedly arranged at the bottom of the tubular connector body, a lantern ring is fixedly arranged in the middle of the tubular connector body, a universal shaping hose is fixedly arranged outside the lantern ring, and a clamp is fixedly arranged at the other end of the universal shaping hose. A fixing ring is arranged at the upper end of the tubular connector in a threaded connection mode, a clamping mechanism used for clamping and fixing an instillation hose is arranged between the upper end of the tubular connector and the inner wall of the fixing ring in a matched mode, and an ultraviolet sterilization mechanism is fixedly arranged at the bottom of the tubular connector. Clamping and releasing of the instillation hose are realized, so that the flushing joint is quickly connected or disconnected; the flushing connector is fixed on the infusion support through the universal shaping hose and the clamp, and the connector is effectively prevented from shaking or making contact with the inner wall of a waste liquid barrel in the flushing process; the liquid outlet is irradiated by the ultraviolet sterilization mechanism, so that possible bacteria can be effectively killed.

The invention discloses a preparation method of a hyper-crosslinked macroporous adsorption resin capable of simultaneously adsorbing bilirubin, bile acid and cytokines in blood purification. The preparation method comprises the following steps: preparing macroporous styrene-divinylbenzene resin with low crosslinking degree from a styrene monomer and a polyethylene phenyl crosslinking agent; then carrying out secondary crosslinking on macroporous styrene-divinylbenzene resin with low crosslinking degree by utilizing a Friedel-Grafts method to prepare hypercrosslinked adsorption resin, and further reacting residual chloromethyl with an amination reagent to improve the adsorption performance of bilirubin and bile acid. The prepared adsorbent can adsorb bilirubin, bile acid and cell factors at the same time, the adsorption rate of the adsorbent to bilirubin is superior to that of an anion resin bilirubin perfusion device used clinically, in combined artificial liver treatment, one perfusion device can replace two perfusion devices, the treatment cost and the operation risk are greatly reduced, and the treatment effect is good. Meanwhile, the biocompatibility is superior to that of anion resin, and the good market application value is achieved.

The utility model discloses a jugular vein artificial hepatic duct fixing device which comprises a bottom plate and extension cloth, an adhesive layer is arranged below the bottom plate, and a fixing belt with a plurality of adjusting holes and fixing pieces capable of being clamped into the adjusting holes and fixed are arranged above the bottom plate. The extension cloth is located on one side of the bottom plate, a wrapping bag is arranged above the extension cloth, and adhesive tapes are arranged on the two sides of the extension cloth. By means of the fixing device, the risks of infection and the like caused by edge curling of the sterile application due to sagging of the double-cavity catheter can be reduced. And a plurality of adjusting holes are formed to adapt to catheter heads with different thicknesses, so that the application range is wide, and the fixation is proper. By arranging the extension cloth and the wrapping bag, the extension tube can be wrapped, the situation that the gauze is frequently replaced due to looseness is avoided, the workload of medical staff can be reduced, and meanwhile the discomfort of a patient can be relieved. The device is not only suitable for treatment of non-biological artificial liver, but also suitable for treatment occasions related to blood purification such as kidney dialysis and the like.

A method of obtaining 3D cell structures including differentiated human hepatic cells. The method includes: a first step of culturing stem cell-derived or immortalized human hepatic progenitors in a non-adherent culture vessel, preferably a low or ultra-low attachment culture vessel; a second step of transferring the stem cell-derived or immortalized human hepatic progenitors to a culture medium including methacrylated gelatin (GelMa), thereby embedding the stem cell-derived or immortalized human hepatic progenitors in a GelMa matrix; and a third step of covering the GelMa matrix with culture medium and culturing the stem cell-derived or immortalized human hepatic progenitors embedded in the GelMa matrix, thereby obtaining 3D cell structures including differentiated human hepatic cells. Also, methods for engineering an artificial liver model or an artificial liver organ, and for assessing in vitro the metabolism, toxicity and / or therapeutic effects of a compound.

The utility model discloses a multi-purpose multi-head clip for combined artificial liver treatment, which comprises a clip body integrally formed by elastic plastics, a first clip head, a second clip head and a third clip head are annularly arranged on the outer side of the clip body at equal intervals, and an infusion line restraining device is arranged at the upper end of the clip body. And one end of the clamp body is connected with a mounting and fixing structure. According to the design, the three chucks are arranged, the three chucks are all made of elastic plastic, the plasma separator, the plasma adsorber and the perfusion device can be conveniently clamped, the front of an artificial liver machine is clean and tidy, protection pads are arranged in the chucks, the effect of protecting the three devices is achieved, and the three chucks have the anti-skid performance and are not prone to falling off. The infusion line restraining device is arranged on the clamp, infusion tubes connected to the plasma separator, the plasma adsorber and the perfusion device can be restrained, site chaos is avoided, and a plurality of clamps do not need to be arranged on an artificial liver machine.