12 results about "Perfusion bioreactor" patented technology

Provided herein are methods of selecting an optimal fixed orientation of a dissolved oxygen (DO) probe in a perfusion bioreactor and culturing a mammalian cell in a perfusion bioreactor having the DO probe positioned at the selected fixed orientation.

A process and system for producing an inoculum for downstream cell production is disclosed. The inoculum is produced in a perfusion bioreactor in which the nutrient media feed is increased as the biomass concentration increases within the bioreactor. A biomass sensor can be used to periodically or continuously monitor biomass concentration. This information can be fed to a controller for automatically increasing nutrient media feed rates in a manner that is directly proportional to producing an inoculum with an increase cell density. The process and system can also include an automated subsystem for maintaining constant volume levels within the perfusion bioreactor during the process.

A method of culturing muscle cells 16 suitable for an edible product comprising: a perfusion module (Fig.2, 2) comprising porous hollow fibres 12; seeding muscle cells 16 onto the fibers 12; maintaini

The present invention provides an apparatus and method for in-well high throughput 3D cell culture, the 3D orifice plate plug-in perfusion bioreactor comprising: an inlet in fluid communication with a first chamber and an outlet in fluid communication with a second chamber; and a plurality of hairy structures extending downwardly from the lower surface of the bioreactor, each hairy structure comprising a supply channel fluidly communicating the first chamber with the hairy structure tip, and a return channel fluidly communicating the second chamber with the hairy structure tip, wherein the supply channel and the return channel are in fluid communication with each other at the tip of the hairy structure. According to the invention, the real biological environment of cells in vivo can be better simulated, which is crucial for realizing accurate drug screening and does not need to sacrifice the high throughput advantage of traditional 2D culture plate screening.

A process and system for producing an inoculum for downstream cell production is disclosed. The inoculum is produced in a perfusion bioreactor in which the nutrient media feed is increased as the biomass concentration increases within the bioreactor. A biomass sensor can be used to periodically or continuously monitor biomass concentration. This information can be fed to a controller for automatically increasing nutrient media feed rates in a manner that is directly proportional to producing an inoculum with an increase cell density. The process and system can also include an automated subsystem for maintaining constant volume levels within the perfusion bioreactor during the process.

A bioreactor system for culturing cells is provided. The bioreactor system includes a vessel comprising an inlet, an outlet, and an internal cavity disposed between the inlet and the outlet and for perfusion of a cell culture medium therethrough. The system further includes a cell culture substrate disposed in the internal cavity in a fixed bed configuration, the surface of the cell culture substrate having a surface area for culturing cells thereon during operation of the bioreactor system. By adjusting the surface area of the cell culture substrate, the bioreactor system can be scaled up or down depending on the number of cells to be cultured.

Provided herein is a perfusion bioreactor having a flow chamber including a flow chamber top, a flow chamber bottom, and an interior space defined between the flow chamber top and the flow chamber bottom. Along the length of the flow chamber, the interior space includes an inlet portion, an outlet portion, and a central portion arranged therebetween. A sealing member is arranged between the flow chamber top and the flow chamber bottom. Further, the inlet portion includes at least one funnel element having a diameter in the transverse direction that increases over a respective length of the inlet portion in the longitudinal direction. A method for culturing cells is also provided herein.

A process and system for producing inoculum for downstream cell production is disclosed. The inoculum is produced in a perfusion bioreactor in which nutrient media feed is increased as biomass concentration within the bioreactor increases. A biomass sensor can be used to monitor biomass concentration periodically or continuously. This information can be supplied to a controller for automatically increasing the nutrient media feed rate in direct proportion to the production of inoculum with increasing cell density. The process and system can also include an automated subsystem for maintaining a constant volume level within the perfusion bioreactor during the process.

The present disclosure relates to a bioreactor assembly having a housing defining an interior chamber; a lid assembly removably coupled to the housing and enclosing the interior chamber; and a gimbal assembly disposable within the interior chamber. The gimbal assembly includes a cradle configured to hold an organ or organ scaffold and an arm assembly configured to move the cradle between a plurality of positions. The bioreactor assembly also includes an ultrasound imaging unit positioned to capture volumetric and / or spatial data of the organ or organ scaffold.

The present disclosure is directed to method (100) for separating a biomolecule from a fluid (2), wherein the biomolecule is produced by biological cells in a perfusion bioreactor (3), the method comprising: forwarding (110) a product fluid comprising the biomolecule and biological cells from the perfusion bioreactor (3) during a bioreactor product output time period; loading (210) a first separation device (4) with the product fluid obtained in step (110) during a first separation loading time period, which constitutes a first part of a first separation cycle time period; pausing (130) the forwarding of the product fluid from the perfusion bioreactor during a bioreactor non-product output time period; and pausing (220) the loading of the first separation device (4) during a second part of the first separation cycle time period. Also disclosed are a computer-implemented method performed by a controller configured to control a separation of a biomolecule from a fluid (2), and further a controller, a computer program, and a computer program product.

A 3D perfusion bioreactor system and method for more accurate modeling of in vivo environmental conditions and cellular behaviors. In some embodiments, the bioreactor includes a cavity for containing culture media and cells. A plurality of capillary tubes cross the cavity. Artery capillary tubes may transport culture media received from an inlet of the bioreactor, across the cavity, and into a return compartment. Vein capillary tubes may transport culture media from the return compartment, across the cavity, and to an outlet of the bioreactor. Metabolites may diffuse from the capillary tubes into the cavity for cellular consumption, and metabolic waste may diffuse from the cavity into the capillary tubes for removal from the bioreactor. In some embodiments, culture media discharged from the outlet may be processed via a waste treatment device and returned to the inlet.

The disclosure provides a filtration system for a cell culture apparatus and a method of cell culture. The filtration system comprises a bioreactor vessel and two or more alternating tangential flow (ATF) filters connected in parallel. A failure in either filter is detected by an in-line sensor, and an automated response system functions to 5 sequester the malfunctioning filter by stopping the flow of liquid media through the filter. Media flow through the remaining operable filters can be increased so that the rate of perfusion through the bioreactor remains relatively unchanged. Such a system may prevent issues that arise from ATF filter failures in conventional perfusion bioreactors, thereby improving the long-term viability of cell cultures. 10 5 10 20 26 20 50 89 29 J un 2 02 6A B S T R A C T O F T H E D I S C L O S U R E 2 0 2 6 2 0 5 0 8 9 2 9 J u n 2 0 2 6 5 1 0