91 results about "Perfusion bioreactor" patented technology

The invention relates to methods of improving protein production, e.g., large-scale commercial protein production, e.g., antibody production, utilizing a modified fed-batch cell culture method comprising a cell growth phase and a polypeptide production phase. The modified fed-batch cell culture method combines both cell culture perfusion and fed-batch methods to achieve higher titers of polypeptide products. Because the modified fed-batch cell culture method of the invention produces higher polypeptide product titers than fed-batch culture alone, it will substantially improve commercial-scale protein production. The invention also relates to a perfusion bioreactor apparatus comprising a fresh medium reservoir connected to a bioreactor by a feed pump, a recirculation loop connected to the bioreactor, wherein the recirculation loop comprises a filtration device, e.g., ultrafiltration or microfiltration, and a permeate pump connecting the filtration device to a permeate collection container.

The present invention includes perfusion bioreactors, automated cell culture systems, and methods for production of cells and cell-derived products.

A novel cell seeded hollow fiber bioreactor is described as a potential bioartificial kidney. Endothelial cells along with pericyte, vascular smooth muscle, and / or mesangial cells or any mesenchymally derived support cells are seeded along a hollow fiber in a perfused bioreactor to reproduce the ultrafiltration function and transport function of the kidney. Maintenance of tissue specific function and ultrastructure suggest that this bioreactor provides an economical device for treating renal failure.

A bioreactor system comprising a multi-well platform including an array of bioreactor units. The bioreactor system comprises a perfusion unit and a fluid source unit fluidly interconnected by a pumping unit. The perfusion unit comprises a multi-well plate including a plurality of main chambers configured to house or contain a cell culture and in each bioreactor unit an independent cell study or experiment may be performed.

A perfusion bioreactor includes at least one ultrasonic transducer that can acoustically generate a multi-dimensional standing wave. The standing wave can be used to retain cells in the bioreactor, and can also be utilized to dewater or further harvest product from the waste materials produced in a bioreactor.

A perfusion bioreactor includes at least one ultrasonic transducer that can acoustically generate a multi-dimensional standing wave. The standing wave can be used to retain cells in the bioreactor, and can also be utilized to dewater or further harvest product from the waste materials produced in a bioreactor.

A perfusion bioreactor includes at least one ultrasonic transducer that can acoustically generate a multi-dimensional standing wave. The standing wave can be used to retain cells in the bioreactor, and can also be utilized to dewater or further harvest product from the waste materials produced in a bioreactor.

The present invention relates to a novel perfusion bioreactor allowing continuous medium feed and extraction of metabolites or other desired products from cells. The invention is useful for plant cell cultures but may also be used for mammalian cell cultures, insect cell cultures and bacterial cell cultures. The design of the reactor includes sedimentation columns mounted inside the bioreactor to separate single cells and cell aggregates from the culture medium at a very low shear stress. The operating conditions allow a stable cell / medium separation by maintaining the medium upward velocity equal to or slightly lower than the cell sedimentation velocity.

A bioreactor system comprising a multi-well platform including an array of bioreactor units. The bioreactor system comprises a perfusion unit and a fluid source unit fluidly interconnected by a pumping unit. The perfusion unit comprises a multi-well plate including a plurality of main chambers configured to house or contain a cell culture and in each bioreactor unit an independent cell study or experiment may be performed.

A novel cell seeded hollow fiber bioreactor is described as a potential bioartificial kidney. Endothelial cells along with pericyte, vascular smooth muscle, and / or mesangial cells or any mesenchymally derived support cells are seeded along a hollow fiber in a perfused bioreactor to reproduce the ultrafiltration function and transport function of the kidney. Maintenance of tissue specific function and ultrastructure suggest that this bioreactor provides an economical device for treating renal failure.

The present invention relates to a method and apparatus for the isolation, modification and re-administration of a molecule or biomolecule, or a class of biomolecules, from the body fluid of a mammal via an extracorporeal closed circuit device. The device is able to capture and modify the biomolecule by the covalent or non-covalent attachment of a secondary molecule or protein, by cross-linking the captured molecule, or by altering the structure of the molecule (for example, by deglycosylation, peptide cleavage, or aggregation). The apparatus can be used to return the modified molecule or biomolecule to the mammalian subject. The device and methods may be utilized for the patient-specific diagnosis and / or treatment of a disease state which presents an associated molecule or protein in plasma or any other fluidized physiological system.The methods and apparatus may also be employed as a closed system allowing the on-line purification and / or modification of a target molecule or biomolecule from a fluid source such as a bioreactor or perfusion bioreactor.

Chemically defined media compositions for the culture of eukaryotic cells are disclosed. The compositions are useful for eukaryotic cell culture in perfusion bioreactors and other vessels.

A perfusion bioreactor includes at least one ultrasonic transducer that can acoustically generate a multi-dimensional standing wave. The standing wave can be used to retain cells in the bioreactor, and can also be utilized to dewater or further harvest product from the waste materials produced in a bioreactor.

The invention relates to a perfusion bioreactor system which comprises a circulating perfusion system, a constant temperature system, a gas exchange system and an on-line monitoring system, wherein the circulating perfusion system comprises a culture chamber, a liquid storage bottle and a peristaltic pump which are sequentially connected through a pipeline to form a perfusion culture loop; the constant temperature system comprises a constant temperature water bath device of which the inside is provided with a circuitous circulating pipeline, and is applicable to an electric heating and refrigerating control part; the gas exchange system is composed of a gas filter, a gas flowmeter, a high-pressure gas bottle, a pressure relief valve and a connecting pipeline; and the on-line monitoring system is composed of a monitoring probe, a monitor terminal, monitoring software, a data conversion chip and a mass transfer regulator. The perfusion bioreactor system provided by the invention can maximally simulate the in-vivo microenvironment in the tissue or organ in-vitro construction process, ensures sufficient substance exchange and normal physiological functions of seed cells in the biological material, enhance the yield of the seed cell / biological stent material, shorten the culture period and lower the culture cost.

The subject invention concerns a perfusion bioreactor device and methods of using the same. A bioreactor device of the invention can be used to grow cells and tissue in a controlled in vitro environment. A perfusion bioreactor device of the invention can have multiple perfusion chambers that can be controlled individually. Transverse or parallel flow of a fluid can be provided to each chamber. Cells can be seeded on a hydrogel and / or 3D scaffold to provide a 3D environment in the bioreactor device where the cells can adhere, proliferate, migrate, secrete growth and / or differentiation factors, and / or undergo differentiation, etc. The subject invention also concerns hydrogels and 3D scaffolds that can be used to grow and / or differentiate cells thereon.

A perfusion bioreactor includes at least one ultrasonic transducer that can acoustically generate a multi-dimensional standing wave. The standing wave can be used to retain cells in the bioreactor, and can also be utilized to dewater or further harvest product from the waste materials produced in a bioreactor.

A perfusion bioreactor includes at least one ultrasonic transducer that can acoustically generate a multi-dimensional standing wave. The standing wave can be used to retain cells in the bioreactor, and can also be utilized to dewater or further harvest product from the waste materials produced in a bioreactor.

The present invention is a holding vessel that has bioreactor and perfusion bioreactor components, a temperature specific environment and holes for transporting substances from a living organism.When the holding vessel is in use it will contain a tissue selection that will be attached to the circulatory system of a living organism by connecting existing vasculature of the organism to engineered or grafted umbilical / vascular cables and then connecting the other end of the umbilical cables to the vasculature of the tissue selection. A tubular construct containing a protective solution will protect the vascular cables.The tissue selections used will be selected from existing or fabricated tissues, but preference is given to cryogenically prepared tissues electronically dispensed from a three-dimensional printing device.

The invention discloses a method for producing PRRS (Porcine Reproductive and Respiratory Syndrome) viruses through culturing Marc-145 cells by applying a torrent-perfusion bioreactor. The method comprises the following steps of: (1) selecting Marc-145 cells as cells for culturing seedlings; (2) subculturing the Marc-145 cells; (3) reproducing virus seeds for production; (4) perfusing and culturing the Marc-145 cells on a paper carrier in the torrent-perfusion bioreactor; (5) producing the PRRS viruses in the torrent-perfusion bioreactor; and (6) treating the obtained virus antigen solution. The invention can greatly decrease the production cost, improve the input-output ratio by more than 10 times and expand the production scale easily and rapidly, has short production preparation cycle and high degree of automation, occupies less area, causes less environmental pollution which is easy to treat, consumes less labor, is easy to realize balanced and stable virus quality and can obviously improve the yield and the quality of the viruses.

A device for biological purposes such as cell culturing, enzymatic reactions or filtering of fluid has a body with first and second surfaces. The body is delimited by a rim and an aperture in the center of the body. The aperture is covered at the first and second surface by first and second plates. The first and / or second plate has an inlet orifice allowing liquid medium into the aperture. Rotating means are arranged in the aperture between the first and second plate. At least one recessed portion is a cavity in the rim of the body having a first outlet orifice allowing the liquid medium to flow out of the body. At least one outlet channel connects the circular aperture with the recessed portion. Liquid is pumped into the aperture of the device and pumped through at least one outlet channel.

The invention relates to the technical field of bioreactors in medical appliances, and more specifically relates to a perfusion type bioreactor of a double-layer circulating system and an application method of the perfusion type bioreactor. The perfusion type bioreactor of the double-layer circulating system comprises a culture tank, an outer layer circulating system arranged outside the culture tank, an inner layer circulating system arranged inside the culture tank, and a gas filling system. By adopting the perfusion type bioreactor, the problem of the contradictory requirement for circulation flow of a culture medium during the culture of cells is solved, the solubility and distribution uniformity of oxygen in the culture medium can be effectively improved, the discharge of the waste gas in the culture medium is facilitated, and air bubbles can be effectively prevented from entering a cell culture area.

The invention belongs to the field of bone tissue engineering and cell mechanics, relating to a dynamic load and recirculating perfusion bioreactor, which comprises a main body, a sliding sleeve module and a three-dimensional culture cabin module, wherein the main body comprises a base, a side support plate and an upper support plate; the side support plate is provided with a concave sliding groove; the sliding sleeve module comprises a sliding sleeve, a piezoelectric ceramic, an up / down adjusting screw and a fastening screw; the sliding sleeve can upward and downward slide in the concave sliding groove stably; the piezoelectric ceramic passes through the sliding sleeve; the three-dimensional culture cabin module comprises a culture cabin main body of which the upper surface is provided with a through hole; the piezoelectric ceramic penetrates into the three-dimensional culture cabin through the through hole, the moving end of the piezoelectric ceramic is connected with a loading rod; and the culture cabin main body is also provided with a liquid inlet and a liquid outlet which are externally connected with a peristaltic pump. The invention has the characteristics of simple operation, high practicability, good aseptic sealing performance, high loading stress output accuracy, and timely and accurate data tracking records.

Disclosed is a novel net member for supporting one or more cell constructs in the culture chamber of a bioreactor, which comprises an array of impermeable pyramidal elements protruding from the face thereof, wherein each of the corners of the base of each of said impermeable pyramidal elements comprises a circular opening. This net member provides for uniform and unobstructed flow of culture medium through 3D cell constructs in culture. Also disclosed are a bioreactor and bioreactor system incorporating said net member. The bioreactor and bioreactor system may be used in the bioproduction of therapeutic proteins and stem cell expansion.

A method of controlling a bioreactor system includes providing a cell culture in a bioreactor, wherein conditions in the bioreactor enable the cell culture to produce a protein of interest (POI), measuring process parameters (PPs) of the culture within the bioreactor by RAMAN, wherein the process parameters are selected from the group consisting of nutrient concentration, viable cell concentration, and protein attributes, measuring a predetermined weight of the bioreactor with the cell culture, removing cell-free spent media from the cell culture using a first output conduit at a first specified rate, removing cells from the cell culture using a second output conduit at a second specified rate, and introducing one or both of fresh media or nutrients into the cell culture using an input conduit at a third specified rate.

The present invention pertains to a system for culturing cells comprising a culturing bag and a continuous flow centrifuge wherein the cells are continuously separated from the supernatant and are recycled into the culturing bag. Further provided are methods for culturing cells and for producing a biological substance using the device for culturing cells, and the use of a bag for culturing cells in said device or said methods. In particular, a perfusion system for culturing cells is provided wherein the wave technology for culturing cells is combined with continuous flow centrifugation for separating the medium from the cells.

A microfluidic system for cell retention for a perfusion bioreactor is provided. The system comprises at least one inlet configured to receive a bioreaction mixture to be processed. At least one curvilinear microchannel is in fluid flow connection with the at least one inlet, the at least one curvilinear microchannel being adapted to isolate cells in the bioreaction mixture, based on cell size, along at least one portion of a cross-section of the at least one curvilinear microchannel. At least two outlets are in fluid flow connection with the at least one curvilinear microchannel. At least one outlet of the at least two outlets is configured to flow the isolated cells to be recycled to the perfusion bioreactor.

The invention relates to a method for culturing mammalian stem cells, in particular embryonic stem cells comprising the following steps: a) providing a perfused bioreactor (1) comprising a cell culture chamber (2); b) placing said mammalian stem cells within said culture chamber (2); c) providing a perfusion loop which provides fresh medium to said perfused bioreactor and removes used medium from said perfused bioreactor; d) providing a dialysis loop which comprises a reservoir of medium (3) and dialysis chamber (4); wherein the dialysis loop provides fresh medium to the perfusion loop through the dialysis chamber (4). The invention also relates to a device for culturing mammalian stem cells according to the invention.

The invention discloses a reciprocally turning perfusion bioreactor for biological artificial liver and a processing method thereof. The invention discloses a bioreactor, which comprises a cylindrical container, wherein the upper and lower ends of the circumferential side wall of the container are both provided with a liquid inlet, and the two end parts of the container both have a liquid outlet. Pressure buffering rings are arranged at the positions of the liquid inlets in the container respectively, and cell screens are arranged at the positions of the liquid outlets at the two ends parts in the container respectively. The invention also provides a reciprocally turning perfusion bioreactor for biological artificial liver, which has the bioreactor, and a processing method thereof. The bioreactor has the advantages that: liver cells cultured on a micro carrier are uniformly distributed in the entire bioreactor; and in a reciprocating motion, the bioreactor causes little damage to the liver cells, allows liver failure plasma to be contacted fully for effectively avoiding perfusion dead cavity and ineffective perfusion, can detoxify plasma with high efficiency, is simple in operation, low in cost and remarkable in effect, and allows the biological artificial liver to play a treatment effect to a maximum degree.

Disclosed herein is a method of examining tissue growth and / or conditioning and / or adhesion properties of cells on a synthetic and / or natural scaffold (30) as well as a perfusion bioreactor (10) for use in this method. The method comprises the following steps: fixing a scaffold (30) to a holding means (28) disposed or disposable in a perfusion bioreactor (10), generating a flow of nutrition solution along the surface of said scaffold (30) held by said holding means (28), generating and / or maintaining physiological conditions in said nutrition solution flowing along said scaffold (30) using heat exchange means (20, 24, 48, 50) and / or gas exchange means (52, 54, 56, 58, 60, 62, 64) associated with said bioreactor (10), such as to allow growth and / or conditioning of cells on said scaffold (30), and optically inspecting, in particular by microscopy, the scaffold (30) held by said holding means (28) through a window (66, 68, 70, 72) provided in said perfusion bioreactor (10) at different times or continuously during tissue growth and / or conditioning of cells on said scaffold (30) while maintaining said physiological conditions in said perfusion bioreactor (10).