41 results about "Microbial inoculation" patented technology

The utility model discloses a kind of placing racks for microbial inoculation tool belonging to the technical field of microbial inoculation, including storage box, the inside of storage box is equipped with placing rack, the middle part of placing rack is equipped with transparent cylinder, one side of placing rack is equipped with connecting plate, the top of connecting plate is equipped with shrinkage groove, the inside of shrinkage groove is equipped with clamping block, the bottom of clamping block is equipped with clamping spring, the middle end of one side of the top of storage box close to connecting plate is equipped with clamping groove, the side of placing rack away from connecting plate is equipped with top plate, the side of top plate is provided with ejector spring, by being provided with placing rack with connecting plate and top plate in the inside of storage box, and using transparent cylinder on placing rack to store inoculation tool, then cooperate clamping block and clamping groove to realize the clamping of connecting plate, ensure the stability of placing rack, so that in inoculation process, inoculation tool can be used alternately, avoid inoculation tool long-term exposure to the outside world, cause the pollution to occur.

The utility model provides a microbial inoculation culture dish bracket for air quality detection, which comprises a supporting rod piece, a telescopic rod which is in sliding connection with the supporting rod piece to form a telescopic structure, a rotating sleeve which is arranged on the upper side of the telescopic rod and is rotationally connected with the telescopic rod, and four supporting rods which are arranged on the peripheral side of the rotating sleeve and are rotationally connected with the rotating sleeve, the connecting rotating sleeve and the supporting rod are used for fixing the positions of the rotating sleeve and the supporting rod; the rotating shafts are rotationally connected with the supporting rods respectively, and bolts are connected between the rotating shafts and the supporting rods in a penetrating mode; the first tray and the second tray are respectively connected with each rotating shaft, a microorganism inoculation culture dish cover is placed in the first tray, and a microorganism inoculation culture dish bottom is placed in the second tray; the two rotating clamping claws are arranged in the first tray; and the pull rod penetrates through the first tray, is connected with the two clamping claws and can drive the clamping claws to clamp and loosen the dish cover of the microbial inoculation culture dish when sliding. The device disclosed by the utility model can be used for saving human resources and improving the precision of experimental data acquisition.

The embodiment of the invention provides an intelligent management and control method and system for rural toilet excrement resource utilization. The method comprises the steps that excrement output and storage state data are collected and uploaded to an Internet of Things platform; dynamic analysis is carried out according to the data, a clearance task scheduling instruction is generated, and the scheduling instruction comprises a clearance point location, clearance time and clearance volume; based on the clearance task scheduling instruction, driving a clearance vehicle to go to a designated rural toilet point location, and synchronously triggering a microbial inoculation program of a pretreatment station, so that the pretreatment station completes microbial inoculation preparation before the clearance vehicle arrives; and monitoring the operation state of the biological treatment link in real time through a big data platform, obtaining treatment parameter data, and feeding back and adjusting microbial inoculation parameters based on the treatment parameter data. Through the scheme of the embodiment of the invention, the excrement cleaning and transporting vehicle can be intelligently scheduled through the Internet of Things and the big data platform, and the microbial inoculation program of the pretreatment station is linked, so that the subsequent biological treatment efficiency is guaranteed.

The invention belongs to the field of biological agriculture fermentation, and particularly relates to snakegourd fruit nutrition powder and a preparation method and application thereof.The snakegourd fruit nutrition powder is prepared by conducting primary subzero treatment on snakegourd fruit roots in liquid carbon dioxide, soaking the snakegourd fruit roots subjected to primary subzero treatment in egg yolk hydrolysate for treatment, soaking the snakegourd fruit roots subjected to hydrolysis egg yolk soaking in liquid nitrogen for secondary subzero treatment, and conducting secondary subzero treatment. Soaking the secondarily cryogenic trichosanthes kirilowii maxim roots in liquid carbon dioxide for three-time cryogenic treatment, and then inoculating a fermentation strain for fermentation, enzymolysis and drying. According to the fermentation method, the fermentation uniformity is good, the trichosanthes kirilowii maxim roots do not need to be crushed, and the problem that the trichosanthes kirilowii maxim roots are difficult to ferment due to the fact that raw materials are compact in structure, microbial inoculation and growth are limited, moisture and ventilation are uneven and the like can be solved by conducting subzero treatment on the trichosanthes kirilowii maxim roots three times in cooperation with egg yolk hydrolysate dipping treatment. The fermented feed liquid obtained by fermenting the trichosanthes kirilowii roots can be prepared into trichosanthes kirilowii nutrition powder, and the fermentation residues can be used as a pig feed additive.

The invention discloses a soil preparation method based on molesey coal gangue and application, and relates to the technical field of solid waste resource utilization and soil improvement. The purpose of the invention is to solve the problems of environmental pollution, resource waste, poor adaptability of coal gangue soil preparation, poor improvement effect and incomplete removal of harmful impurities caused by accumulation of the mossy system coal gangue in the prior art. The method comprises the following steps: 1, pretreating the molery-series coal gangue; 2, mixing and improving; 3, microbial inoculation and curing; and 4, planting adaptive adjustment. The prepared soil is applied to mine land reclamation and agricultural planting soil improvement. According to the method, resource utilization of the mossy system coal gangue is effectively achieved, the prepared soil has the advantages of being appropriate in fertility, good in water and fertilizer retention, free of secondary pollution and the like, meanwhile, the soil preparation cost is reduced, remarkable economic, social and environmental benefits are achieved, and the method can be widely applied to the fields of mining area land reclamation, agricultural planting soil improvement and the like.

The invention provides a compound self-disinfection inoculation device with multiple inoculation ends, which relates to the technical field of microbial inoculation and comprises an inoculation needle cylinder, an ultraviolet disinfection component arranged in the inoculation needle cylinder and an alcohol disinfection component communicated with the ultraviolet disinfection component, a conveying tube is arranged in the inoculation needle cylinder; two ends of the inoculating needle cylinder are respectively provided with a connecting end and a needle outlet end; the alcohol disinfection component is arranged in the connecting end, a button is arranged on one side of the connecting end, the working face on one side of the button is connected with a connecting rod, the side, close to the ultraviolet disinfection component, of the button is connected with an extension rod, and one end of the extension rod is provided with a preset contact slope; a pushing part is arranged in the ultraviolet disinfection part; a cutting part is arranged on the working surface of the top end of the pushing part; through efficient sterilization of ultraviolet rays and rapid disinfection of alcohol, a compound disinfection system is formed, and the risk of cross infection in the inoculation process is reduced.

The utility model discloses a mixed microorganism inoculation fermentation device of intelligent control bacteria, including the fermentation cylinder of setting, install motor on the fermentation cylinder, motor output end is connected with first rotating shaft, the eccentric wheel is installed to first rotating shaft outside, the inside setting of fermentation cylinder has the guide rod, the mobile board is arranged to guide rod outside, the rack is installed to mobile board side end, the gear is arranged to rack side end, the second rotating shaft is passed through and is connected to the inside of gear, the screw piece is arranged to second rotating shaft outside. This mixed microorganism inoculation fermentation device of intelligent control bacteria, through the first rotating shaft outside first mixing piece, mixes microorganism, makes different bacteria evenly distribute, promotes the material exchange and information transmission between microorganism, the screw piece of second rotating shaft outside rotates, has reduced the problem that the mixing efficiency is not high because of single stirring, has further improved the evenness of microorganism mixing, has improved the growth speed and yield of overall flora.

This invention relates to the field of food biotechnology and fermentation microbiology, specifically a method for obtaining lactic acid bacteria (LAB) isolates as starter culture candidates. The method is carried out by preparing a wheat-based fermentation substrate and adding gelatin protein as a nitrogen source to form a fermentation medium of wheat, gelatin, and fructose. Indigenous microorganisms in the substrate are inoculated into the fermentation medium and incubated under conditions that support the growth of lactic acid bacteria until lactic acid bacteria isolates are obtained. Fermentation is carried out at a temperature of 30–37°C for 12–72 hours, with a gelatin concentration of 10% (w / v) and a wheat to water ratio of 1:1 to 1:5 (w / v). The fermentation medium, a combination of wheat, gelatin, and fructose, functions as a selective medium for growing and selecting lactic acid bacteria before further isolation on standard microbiological media.This method produces lactic acid bacteria isolates that can be used as starter culture candidates in food fermentation processes.

The invention discloses a layered gel culture technology constructed by using environmental extracts, and belongs to the field of environmental microorganism culture. According to the technology, an environmental sample is separated into a particle phase and a liquid phase through water extraction, the particle phase and the liquid phase are respectively prepared into a lower-layer solid-phase matrix and an upper-layer gel phase with gel (preferably agar), a vertical nutrition / signal slow-release gradient is formed in a culture dish, and particle attachment sites are provided; and inoculating the environmental microorganisms on the upper layer or the surface for culture. Compared with nutrient agar or extremely simple water agar, the method has the advantages that more bacterial colonies and higher distinguishable form number can be obtained under the repeatable condition, and the community diversity and the OTU / ASV overlapping degree with an original sample are improved; rare / slow-growing groups can be stably separated, and subsequent 16S / metagenome analysis and agar block bacteriostasis primary screening are facilitated. Meanwhile, the invention provides a corresponding culture medium composition and a kit / prefabricated layered culture dish. According to the scheme, operation is easy and convenient, parameters can be standardized, and the method is suitable for isolated culture and ecological function research of environmental samples such as soil, sediments and low-solid water samples.

The utility model belongs to the technical field of biological evaluation of medical instruments, and particularly relates to a wound biological membrane culture system and a dynamic in-vitro wound model for antibacterial activity evaluation, which comprise a culture medium dripping system, a reactor and a waste liquid collecting bottle which are sequentially connected, according to the utility model, the glass slide and the in-vitro pigskin tissue or the glass slide and the in-vitro pigskin tissue, the test dressing and the balancing weight are sequentially arranged in the test bin of the reactor from bottom to top. The in-vitro pigskin tissue is used for wound surface construction and microbial inoculation, continuous nutrition supply is provided, the in-vitro pigskin tissue in-vitro biological membrane preparation device can be used for preparing a wound surface biological membrane, planktonic bacteria infection or biological membrane infection of the wound surface can be simulated, the in-vitro pigskin tissue in-vitro biological membrane preparation device is closer to the actual clinical condition, and the antibacterial activity of the antibacterial wound surface dressing can be predicted more accurately.

This invention relates to the field of river water body treatment and maintenance technology, and in particular to a river water body treatment and maintenance device and method, comprising a garbage collection unit, a garbage storage unit, a microbial inoculation unit, and a drive unit. The garbage collection unit is located at the front end of the maintenance device, the garbage storage unit is located behind the garbage collection unit, and the microbial inoculation unit is located on one side of the garbage storage unit. The drive motor of the drive unit is located at the end of the maintenance device, and the airbag of the drive unit is located at the bottom of the maintenance device. This invention employs the above-mentioned river water body treatment and maintenance device and method, which can automatically collect and store floating garbage on the surface of the river water, and then treat and maintain the river water by inoculating a compound microbial agent.

This invention belongs to the field of *Coptis chinensis* cultivation technology, specifically relating to a cultivation method for increasing the berberine content in *Coptis chinensis*, comprising the following steps: seedling cultivation, transplanting, microbial inoculation, light management, fertilization management, staged drought stress induction, water management, and harvesting; the microbial inoculation involves simultaneously applying a compound microbial agent containing *Bacillus amyloliquefaciens*, arbuscular mycorrhizal fungi, and plant rhizosphere growth-promoting bacteria at the time of transplanting; the light management involves shading combined with LED supplemental lighting; the staged drought stress induction involves controlling the relative soil moisture content to 45%-55% for 7-10 days after transplanting, 60-75 days after transplanting. This invention, through the synergistic effect of microbial inoculation, light quality regulation, and drought stress induction, can significantly increase the berberine content in the roots of *Coptis chinensis* by more than 120% compared to conventional cultivation methods, and is standardized, green, and sustainable, suitable for large-scale cultivation and promotion.

The application discloses a kind of plant synergistic microbial power generation methods, including preparation membrane electrode, preparation plant material, electrode and plant tissue coupling, reference electrode connection, circuit connection, microbial inoculation, enhance microbial activity and fuel cell start-up etc., the application gets rid of the dependence on water environment, make the operation of plant power generation greatly reduce the influence of environmental factors, power output is more stable, thereby improve power generation efficiency and reliability.Secondly, the application expands the range of coupled plants.In addition, the application innovatively introduces microporous filter membrane between membrane electrode and plant tissue, optimizes the contact and material exchange between electrode and plant tissue, which helps to improve the performance of electrode and the power generation efficiency of fuel cell.

The invention provides a method and system for detecting the carbon utilization rate of microorganisms based on a microwell plate respiratory system, and the method comprises the following steps: pre-culturing target microorganisms, and confirming the logarithmic growth phase of the target microorganisms; the method comprises the following steps: inoculating target microorganisms into a plurality of deep holes of a microporous plate respiratory system for culture, wherein each deep hole in the microporous plate respiratory system comprises a bacterial liquid containing a specified carbon source; detecting the initial biomass in the bacterial liquid and the initial carbon dioxide value in the microplate respiratory system at the beginning of culture, and detecting the final biomass in the bacterial liquid and the final carbon dioxide value in the microplate respiratory system at the end of culture; calculating the growth rate of the target microorganisms according to the initial biomass and the end biomass; calculating the respiration rate of the target microorganism according to the initial carbon dioxide value, the end carbon dioxide value and the growth rate; and calculating the carbon utilization rate of the target microorganism according to the growth rate and the respiration rate. The problem that the carbon utilization rate of species level microorganisms is difficult to obtain is solved.

The invention discloses a microbial electrolysis cell device and method for purifying phosphorus pollution by utilizing agricultural waste biochar. The device comprises an electrolytic tank main body (1) and a circulating system. The electrolytic tank main body (1) is of a single-chamber structure, and the constant-voltage power supply (12) is connected with the anode bar (2) and the cathode bar (3). And the cathode bar (3) adopts a composite structure of a permeable electrode shell (31) and a biochar electrode (32), so that the electrode is convenient to replace and recycle. The device can be further provided with a monitoring system and a reaction maintaining system, and automatic regulation and control are achieved. The method comprises the steps of microbial inoculation and biofilm formation, pretreatment feeding, calcium source supplementation, electrolytic adsorption coupling phosphorus removal, calcium phosphate recovery and the like, and efficient phosphorus removal and phosphorus resource recovery are achieved through the synergistic effect of electrochemistry and biological adsorption under the condition of low voltage (0.5-1.5 V). The method is suitable for treating multi-source phosphorus-containing wastewater in agriculture, industry, life and the like.

The invention discloses a biological treatment and recycling method for synchronously removing carbon and enriching lithium from oil and gas field produced water, which comprises the following steps: 1) inoculating microorganisms into a liquid culture medium, and culturing to obtain a microorganism culture solution or culture; 2) directly adding and / or treating the microbial culture solution and / or culture obtained in the step 1) and then adding the microbial culture solution and / or culture into a reactor filled with oil and gas field produced water to be treated; (3) operating the reactor in the step (2), degrading organic matters in the oil and gas field produced water by microorganisms and enriching lithium ions; and 4) separating the microorganisms from the oil and gas field produced water, and extracting lithium ions enriched in the microorganisms. Wherein the microorganism is selected from one or a combination of more of the following microorganisms: staphylococcus saprophyticus, penicillium fistulosum, bacillus altitudinis, alternaria tenuissima and aspergillus sydowii. According to the method, recycling of lithium is achieved while oil and gas field produced water is recycled / discharged after reaching the standard, and the method has important significance on the national ecological environment and the new energy strategy.

The application provides a forest small and micro water source group community construction method and system based on an ecological chain, relates to the technical field of ecological engineering, and comprises the following steps: selecting a natural catchment area according to forest topographic features, constructing a water area unit with a layered anti-seepage structure, arranging an anti-seepage layer at the bottom to realize water collection, and arranging a water-permeable transition layer at the edge to form a water-land gradient; dividing the water area unit into multiple ecological function areas according to the water depth gradient and spatial position and introducing matching species; dynamically adjusting the species ratio and spatial layout of adjacent function areas based on the functional correlation of material circulation and energy transmission by monitoring the population density and water quality indexes, forming a self-sustaining ecological chain; arranging a microorganism cultivation layer around the periphery, calculating the inoculation proportion of saprophytic microorganisms, calculating the soil organic matter conversion amount according to the population density and decomposition rate, and establishing the dynamic balance of the water supply amount of the water area, the metabolic activity of microorganisms and the physical and chemical properties of forest soil. The water source ecological system constructed by the application has high stability, can effectively improve the water holding capacity of forest soil and reduce the risk of fire.

This invention relates to the field of solid waste application technology, and particularly to a method for improving the stability of steel slag based on dissolved oxygen regulation using microorganisms. The method includes the following steps: crushing and screening waste steel slag, washing, drying, sterilizing, and drying to constant weight to obtain steel slag aggregate; preparing a liquid culture medium, adjusting the pH value of the liquid culture medium to be approximately equal to the pH value of the steel slag aggregate; inoculating microorganisms into the liquid culture medium for cultivation; centrifuging the cultured microbial solution; diluting the bacterial sludge with 0.9% physiological saline or resuspending it with a sterilized 5g / L yeast extract solution; adjusting the OD600 value of the bacterial solution to 0.8~1.2; using a vacuum negative pressure adsorption device and surface spraying to ensure full contact between the bacterial solution and the steel slag aggregate and adsorption to saturation; preparing a mineralizing nutrient solution to mineralize the steel slag aggregate after adsorption of the bacterial solution, resulting in stabilized steel slag aggregate. This invention utilizes a microbial dissolved oxygen response regulation strategy to maximize and improve the stabilization efficiency of microbially modified steel slag.

The invention discloses a method for relieving continuous cropping obstacles of Tibetan medicine continuous cropping land, and relates to the field of agricultural planting. Comprising the following steps: soil deep ploughing and matrix adding: performing deep ploughing and upturned soil airing on a target land parcel at the depth of 30-40cm, after airing, uniformly spreading a decomposed sheep manure organic fertilizer according to the dosage of 200-300kg per mu, and spreading plant ash according to the dosage of 80-120kg per mu; inoculating with a specific microbial agent: uniformly mixing a compound microbial agent with fine moisture soil, and uniformly spreading the mixture on the surface of the treated soil; after the step S2 and the step S3 are completed, a degradable material is adopted for conducting full ground covering, the covering time is 25-35 days, and the soil humidity is kept to be 50%-60% of the field moisture capacity in the period; planting and growing period management: ditching or digging holes after uncovering the film. According to the method, the problem of continuous cropping obstacles is systematically solved through a three-in-one technical path of bioremediation, soil improvement and ecological regulation and control.

The invention provides a treatment method for synergistic aerobic composting of faeces and urine-containing tofu cat litter and garden waste. The method specifically comprises the following steps: 1) raw material preparation, 2) functional flora inoculation, 3) composting facility preparation and adjustment, and 4) composting process control and management. The tofu cat litter and the garden waste are reasonably proportioned, and the carbon nitrogen ratio, the water content, the composting temperature and other key parameters are regulated and controlled, so that the problems that the manure-containing cat litter is difficult to stably compost, high in pathogen risk, heavy in odor and the like are effectively solved, safe and efficient resource utilization of the manure-containing cat litter is realized, the process is simple, and the method is suitable for small-scale or community-level popularization and application.

The utility model discloses an inoculation tool for claviceps purpurea experiment, which relates to the technical field of microbial inoculation and comprises a rod head and a rod body, the rod head and the rod body are integrally formed, a plurality of vent holes are arranged on the rod head, an elastic sleeve is sleeved outside one end of the rod body far away from the rod head, and an anti-pollution baffle is slidably sleeved on the rod body. The rod head and the rod body which are integrally formed are made of metal and can be sterilized at high temperature, the rod body is thin and long, so that the rod body can easily enter and exit from the inclined plane opening, the anti-pollution baffle is arranged on the extension rod section, the risk of pollution caused by long-time operation can be prevented, the handheld rod section is provided with the elastic sleeve, an operator can easily grasp the rod without slipping, and the operation is convenient. Most importantly, the serrated rod head end can easily separate tough claviceps purpurea, so that the claviceps purpurea stripping and inoculating efficiency is improved, and time and labor are saved.

The invention is applicable to the technical field of microbial engineering, and relates to a method for producing microbial protein by reinforcing synthesis gas, which comprises the following steps: heating, drying and calcining an iron source precursor solution, and grinding to obtain iron-based catalyst solid particles; the method comprises the following steps: inoculating aerobic microorganisms into a culture medium, and introducing synthesis gas for culture and domestication to obtain activated aerobic microorganisms; loading the obtained iron-based catalyst solid particles into a titanium mesh, and then physically mixing the iron-based catalyst solid particles with the activated aerobic microorganisms to obtain an aerobic fermentation system; and continuously introducing synthesis gas into the reactor containing the aerobic fermentation system, culturing under an aerobic condition, growing aerobic microorganisms, and synthesizing a microbial protein product. According to the invention, the iron-based catalyst and the Fe microelement are added and used, so that the growth of microorganisms is promoted, the electron transfer process of HOB can be promoted, and the problem of low protein yield caused by toxicity inhibition of synthesis gas on the growth of microorganisms in the prior art is effectively solved.

This utility model discloses a microbial inoculation device for organic fertilizer production, specifically relating to the technical field of microbial inoculation devices. It includes a conveying pipe, a spiral conveying rod, and a feeding hopper. A first motor is installed on the left side of the conveying pipe, with its output end extending into the interior of the pipe. A spiral conveying rod is installed at the output end of the first motor. A feeding hopper is installed on the left side of the top of the conveying pipe. A through groove is provided on the surface of the bottom right side of the conveying pipe. A tail box is installed on the right side of the conveying pipe, and a throwing assembly is provided at the bottom of the tail box. This utility model, by incorporating a throwing plate, allows the second motor to be activated when the feed material enters the discharge pipe along the through groove and drop chute. This drives the rotating shaft to rotate the throwing plate. During this rotation, the throwing plate contacts the falling microbial material, throwing it outwards along the throwing chute. This increases the distribution range of the microbial material and enhances the application range of the microbial inoculation device for organic fertilizer production.

This invention belongs to the field of ecological restoration technology and discloses a temperature-controlled ecological soil conditioner, its preparation method, and its application. The preparation method of the temperature-controlled ecological soil conditioner includes the following steps: S1, taking undehydrated municipal sludge in muddy state, removing impurities, adding a heavy metal passivating agent, and stirring to react, obtaining pretreated municipal sludge; S2, mixing the pretreated municipal sludge with sawdust, fly ash, and temperature-controlled biochar, then stirring evenly to obtain a mixture; S3, adding a compound fermentation agent to the mixture, mixing evenly, and then performing self-heating composting fermentation. After fermentation, the product is crushed, sieved, and then aerated and aged to obtain the temperature-controlled ecological soil conditioner. This invention uses undehydrated municipal sludge as the main raw material, supplemented with sawdust, fly ash, and temperature-controlled biochar, and through heavy metal passivation, microbial inoculation, and multi-stage self-heating composting, prepares a green soil conditioner that combines high-efficiency fermentation, ecological safety, and soil compatibility.

The invention relates to the technical field of anaerobic fermentation, in particular to a method for producing lactobacillus reuteri and acetic acid by promoting anaerobic fermentation through a low-intensity magnetic field. The invention aims to solve the technical problem that lactobacillus reuteri and acetic acid cannot be efficiently and stably produced in a low-cost, pollution-free, easy-to-operate and high-specificity manner in the prior art. According to the technical scheme, the method for producing the lactobacillus reuteri and the acetic acid comprises the following steps: mixing organic wastes and microbial inoculums to obtain a mixture; adjusting the total solid content of the mixture to obtain a fermentation reactant; and fermenting the fermentation reactant, and applying a magnetic field to obtain the lactobacillus reuteri and acetic acid. The method is mainly used for providing a new choice for the production method of the lactobacillus reuteri and the acetic acid, so that the lactobacillus reuteri and the acetic acid are efficiently and stably produced in a low-cost, pollution-free, easy-to-operate and high-specificity manner.

The invention discloses an ecological restoration method for high and cold mining wasteland, and belongs to the technical field of ecological restoration of mining areas. The ecological restoration method for the high and cold mining wasteland comprises the following steps: (1) surveying and quantifying the field; (2) landform improvement and soil pretreatment; (3) soil improvement; (4) inoculating functional microorganisms; (5) coldproof plant configuration and habitat maintenance. The invention provides a comprehensive remediation method integrating cold-resistant plant screening, soil matrix targeted improvement, functional microorganism and plant coupling remediation and auxiliary engineering measures, and aims to effectively solve the key problems of low vegetation survival rate, soil depletion, poor matrix stability, limited microbial activity and the like in ecological remediation of high and cold mining areas. And rapid, stable and sustainable ecological restoration of waste land is realized.

The utility model relates to a scribing mechanism, and relates to the technical field of microbe inoculation scribing equipment, the scribing mechanism comprises a base, an inoculation needle placed on the base and a three-axis driving module for driving the inoculation needle to move, and the three-axis driving module is connected with the inoculation needle through a connecting assembly and is used for driving the inoculation needle to move along the X-axis direction, the Y-axis direction and the Z-axis direction. The inoculating needle is located on the base before scribing, the three-axis driving module drives the inoculating needle to be separated from the base through the connecting assembly, under the action of the three-axis driving module, the inoculating needle moves in the X-axis direction, the Y-axis direction and the Z-axis direction to achieve sampling and scribing, and after scribing is completed, the three-axis driving module drives the inoculating needle to move back to the base. In this way, mechanical microbial inoculation lineation operation is completed, manual inoculation needle mounting and dismounting are not needed, and therefore the convenience of lineation operation is improved.

The invention provides a forest small and micro water source community construction method and system based on an ecological chain, and relates to the technical field of ecological engineering, and the method comprises the steps: selecting a natural water collection area according to forest topographic features, constructing a water area unit with a layered anti-seepage structure, arranging an anti-seepage layer at the bottom to achieve water collection, and arranging a permeable transition layer at the edge to form a land and water gradient; dividing the water area unit into a plurality of ecological functional areas according to the water depth gradient and the spatial position, and introducing matched species; by monitoring population density and water quality indexes, on the basis of functional association of material circulation and energy transfer, the species ratio and spatial layout of adjacent functional areas are dynamically adjusted, and a self-sustaining ecological chain is formed; arranging a microbial cultivation layer on the periphery, calculating the inoculation proportion of the saprophytic microorganisms, calculating the soil organic matter conversion amount according to the population density and the decomposition rate, and establishing dynamic balance of the water area water supply amount, the microbial metabolic activity and the forest soil physicochemical property. The constructed water source ecological system is high in stability, the water holding capacity of forest soil can be effectively improved, and the fire risk is reduced.

The invention discloses a preparation method of an autotrophic nitrogen removal biological filter material and a synchronous nitrogen removal process. The preparation method specifically comprises the following steps: step 1, preparing raw materials; step 2, mixing and granulating; 3, high-temperature roasting; step 4, surface modification; step 5, drying treatment; step 6, inoculating microorganisms; step 7, performance detection and screening; the autotrophic nitrogen removal biological filter material prepared by the preparation method disclosed by the invention has a rich pore structure, good biocompatibility and relatively high mechanical strength. The pore structure is beneficial to attachment and growth of microorganisms, the biocompatibility can promote enrichment of autotrophic nitrogen removal microorganisms, the mechanical strength ensures that the filter material is not easy to break in a long-term operation process, and the service life of the filter material is prolonged. The synchronous denitrification process based on the biological filter material can realize efficient denitrification in treatment of sewage with low carbon-nitrogen ratio.

The invention discloses a fermentation method and application of a microbial agent in a field, and belongs to the technical field of agricultural microbial fermentation. According to the fermentation method, microorganisms are inoculated into the fermentation device for field fermentation according to the proportion, and the microbial agent is obtained after fermentation is finished. The fermentation device disclosed by the invention can be applied to fields, is small, simple, intensive, light and portable, has relatively high environmental adaptability and operation convenience, can regulate and control fermentation conditions, shorten fermentation time and improve microbial agent propagation efficiency, can also adapt to fermentation of different agricultural microorganisms and different weather conditions, and has wide application prospects. Meanwhile, the device is provided with a defoaming device capable of automatically adding a defoaming agent, and is suitable for fermentation of microbial inoculum which continuously generates foam; and the final fermentation product can be introduced into a water-fertilizer integrated system, so that water-fertilizer integration is realized, and the cost is reduced in the aspects of production, labor and time. Therefore, the fermentation device and the fermentation method have wide market requirements and good application prospects.