97 results about "Microbial inoculation" patented technology

The invention relates to the technical field of biological improvement methods, and discloses a comprehensive biological improvement method for severe saline-alkali grassland. According to the method, S1, early evaluation and planning are conducted, S2, straw returning to the field and soil preliminary conditioning are conducted, S3, shallow ploughing and soil preparation operation is conducted, S4, microbial inoculation is conducted to strengthen soil activity, S5, pioneer plant sowing and planting are conducted, S6, precise irrigation and drainage regulation and control are conducted, and S7, long-term monitoring and later-period management and maintenance are conducted, through straw returning to the field and microbial inoculation, the pH value of soil can be reduced to 8.0-8.5 from the initial 8.5-10.5, and the salt content is reduced to 0.5%-1% from 1%-3%; compared with the prior art, the method has the advantages that the alkalinization degree is obviously reduced, meanwhile, the organic matter content is increased from lower than 1% to 2%-3%, the soil structure is improved, the porosity is increased, the air permeability and the water permeability are enhanced, a good soil environment is created for plant growth, for example, humus generated by straw decomposition can promote soil particle aggregation, a stable aggregate structure is formed, and the water and fertilizer retention capacity of soil is improved.

The invention provides a post-fire soil remediation method. The post-fire soil remediation method comprises the following steps: step 1, fire soil diagnosis and pretreatment; 2, gradient pyrolysis biochar is prepared and applied; step 3, inoculating with a compound microbial agent; 4, nutrient regulation and plant combined remediation. According to the invention, through combined application of gradient pyrolysis biochar, cooperative regulation and control of soil acidity and alkalinity, heavy metals and organic pollutants after a fire disaster are realized, and meanwhile, the survival rate and colonization ability of functional microorganisms in a high-temperature environment are remarkably improved by adopting a carrier embedding technology. According to the method, the remediation period can be effectively shortened, the ecological function of the soil is remarkably restored, the remediation cost is greatly reduced by using localized materials, and the problems that in a traditional remediation technology, adaptability is poor, efficiency is low, and cost is high are solved on the whole.

The invention provides self-repairing foam concrete and a preparation method thereof, and belongs to the technical field of concrete. The preparation method comprises the following steps: carrying out hydroxylation treatment on stone powder to obtain hydroxyl stone powder, modifying sodium alginate by using a silane coupling agent to obtain a grafted copolymer, compounding the hydroxyl stone powder, the grafted copolymer and nano silicon dioxide to obtain a composite carrier, and inoculating bacillus pasteurii onto the composite carrier; 300-400 parts by mass of cement, 100-150 parts by mass of the composite carrier inoculated with the bacillus pasteurii, 1 part by mass of a composite foaming agent and 10 parts by mass of urea are mixed according to the water-binder ratio of 0.35-0.45, and the self-repairing foam concrete is obtained. The self-repairing foam concrete is prepared by a one-step method through an innovative microbial carrier design and a composite repairing mechanism, and the problem that microbial inoculation and self-repairing foaming in composite concrete in the prior art need to be carried out step by step is solved.

The invention relates to a method for in-situ synthesis of a bacterial cellulose membrane by using a hippophae rhamnoides fruit juice additive, which comprises the following steps: juicing hippophae rhamnoides fruits to obtain fruit pulp, mixing the fruit pulp with deionized water, filtering to obtain hippophae rhamnoides fruit juice, adding the hippophae rhamnoides fruit juice into a culture medium, inoculating microorganisms into the culture medium, and further synthesizing the bacterial cellulose membrane. The bacterial cellulose membrane synthesized by the method is of a three-dimensional network structure, and the average diameter is 10-100nm; x-ray diffraction characterizes that the bacterial cellulose added with different hippophae rhamnoides fruit juice has a cellulose I structure, and has three characteristic diffraction peaks at 2theta of 14.5 degrees, 16.7 degrees and 22.7 degrees. Through jade software calculation, it is found that the crystallinity of the bacterial cellulose added with the hippophae rhamnoides fruit juice is not greatly different from that of the bacterial cellulose not added with the The synthesis method is simple, convenient, efficient, green and environmentally friendly, the yield of the bacterial cellulose can be remarkably increased, and the obtained bacterial cellulose membrane is stable in structure and good in crystallinity and has good application prospects.

The utility model discloses a kind of placing racks for microbial inoculation tool belonging to the technical field of microbial inoculation, including storage box, the inside of storage box is equipped with placing rack, the middle part of placing rack is equipped with transparent cylinder, one side of placing rack is equipped with connecting plate, the top of connecting plate is equipped with shrinkage groove, the inside of shrinkage groove is equipped with clamping block, the bottom of clamping block is equipped with clamping spring, the middle end of one side of the top of storage box close to connecting plate is equipped with clamping groove, the side of placing rack away from connecting plate is equipped with top plate, the side of top plate is provided with ejector spring, by being provided with placing rack with connecting plate and top plate in the inside of storage box, and using transparent cylinder on placing rack to store inoculation tool, then cooperate clamping block and clamping groove to realize the clamping of connecting plate, ensure the stability of placing rack, so that in inoculation process, inoculation tool can be used alternately, avoid inoculation tool long-term exposure to the outside world, cause the pollution to occur.

The invention relates to a microbial exopolysaccharide for reducing soil porosity, a preparation method and application. The preparation method comprises the following steps: (1) inoculating microorganisms into a culture medium A, and culturing to obtain a seed solution; inoculating the seed solution into a culture medium B, culturing to obtain fermentation liquor, centrifuging to obtain fermentation liquor supernate, and performing rotary evaporation concentration to obtain concentrated liquor; (2) mixing the concentrated solution with ethanol, and centrifuging to obtain a polysaccharide precipitate; adding water into the polysaccharide precipitate, stirring and dissolving, dialyzing, and carrying out vacuum freeze drying to obtain crude polysaccharide; and (3) preparing the crude polysaccharide into a crude polysaccharide solution, then performing chromatographic separation, gradient elution and vacuum freeze drying, dialyzing, and then performing freeze drying to obtain the microbial exopolysaccharide. The microbial exopolysaccharide is good in rheological property, high in viscosity, simple to prepare, capable of being produced on a large scale, capable of effectively reducing the porosity of media such as soil and quartz sand and capable of being applied to the field of environmental remediation.

The utility model provides a microbial inoculation culture dish bracket for air quality detection, which comprises a supporting rod piece, a telescopic rod which is in sliding connection with the supporting rod piece to form a telescopic structure, a rotating sleeve which is arranged on the upper side of the telescopic rod and is rotationally connected with the telescopic rod, and four supporting rods which are arranged on the peripheral side of the rotating sleeve and are rotationally connected with the rotating sleeve, the connecting rotating sleeve and the supporting rod are used for fixing the positions of the rotating sleeve and the supporting rod; the rotating shafts are rotationally connected with the supporting rods respectively, and bolts are connected between the rotating shafts and the supporting rods in a penetrating mode; the first tray and the second tray are respectively connected with each rotating shaft, a microorganism inoculation culture dish cover is placed in the first tray, and a microorganism inoculation culture dish bottom is placed in the second tray; the two rotating clamping claws are arranged in the first tray; and the pull rod penetrates through the first tray, is connected with the two clamping claws and can drive the clamping claws to clamp and loosen the dish cover of the microbial inoculation culture dish when sliding. The device disclosed by the utility model can be used for saving human resources and improving the precision of experimental data acquisition.

The invention discloses a remediation method and system for soil heavy metal pollution based on a bamboo nano material, bamboo is cut in the axial direction of fibers, ultrasonic cleaning and vacuum drying are carried out, after energy gradient grinding is carried out in sequence, high-pressure homogenization and in-situ dispersion nanocrystallization treatment are carried out, and the porous bamboo nano material is obtained; the method comprises the following steps: inserting an anti-heavy metal target gene into a selected microbial expression vector, introducing the expression vector into microorganisms by using an electroporation method, detecting the expression level of the target gene, inoculating the microorganisms over-expressing the target gene into a culture medium, and determining the heavy metal removal rate based on microbial growth and the heavy metal removal rate. The microorganism with high heavy metal tolerance and biotransformation capability is obtained; the method comprises the following steps: uniformly distributing microorganisms under the assistance of a dispersing aid, carrying out low-temperature standing crosslinking by adopting a cross-linking agent, adsorbing the microorganisms on the surface of a bamboo nano material to obtain a microorganism and bamboo nano material composite repairing agent, and applying the microorganism and bamboo nano material composite repairing agent to polluted soil for heavy metal pollution repairing.

The invention provides a biochemical combination method for neutralizing bromine-containing brine, which comprises the following steps: adding alkaline chemical substances into bromine-extracted brine, and adjusting the pH value to 5 or more; culturing algae and / or microorganisms by using a nutrient solution with the same salinity as the brominated brine; inoculating the algae or microorganisms cultured in the step S2 into the brine with the pH adjusted in the step S1, and adding nutrient elements required by the growth of the cultured algae or microorganisms; and culturing until the pH value of the brine reaches 7 or above. According to the biochemical combination method for neutralizing bromine brine, the pH of bromine extraction waste liquid reaches 5 or above by adding alkaline substances in a chemical and biological combination mode, then algae and microorganisms are inoculated and cultured, the pH of the brine is 7 or above by means of metabolite of the microorganisms and photosynthesis of the algae, the use amount of the alkaline substances is effectively reduced, and the utilization rate of the alkaline substances is increased. Meanwhile, the brine ecology is recovered, and subsequent comprehensive utilization of the brine is facilitated.

The present disclosure discloses a method for strengthening a biological manganese oxidation using a magnetic field and use thereof. The method includes steps of inoculating a manganese-oxidizing microorganism into a culture medium containing Mn2+, performing magnetization treatment in a culture process, and then collecting a biogenic manganese oxide. The method includes steps of performing a primary magnetic field treatment at a magnetic field intensity of 0.2-50 mT for 1-5 h when culturing is performed for 6-12 h, continuing culturing after the primary magnetization treatment, and performing magnetization treatment once every other 24 h for culture time of 72 h. A magnetic field is applied to accelerate an oxidation rate of a manganese-oxidizing microorganism to Mn2+ and a biological manganese oxidation rate is respectively improved by 36.4% and 23.8% under an action of an alternating magnetic field or a constant magnetic field within 72 h.

The embodiment of the invention provides an intelligent management and control method and system for rural toilet excrement resource utilization. The method comprises the steps that excrement output and storage state data are collected and uploaded to an Internet of Things platform; dynamic analysis is carried out according to the data, a clearance task scheduling instruction is generated, and the scheduling instruction comprises a clearance point location, clearance time and clearance volume; based on the clearance task scheduling instruction, driving a clearance vehicle to go to a designated rural toilet point location, and synchronously triggering a microbial inoculation program of a pretreatment station, so that the pretreatment station completes microbial inoculation preparation before the clearance vehicle arrives; and monitoring the operation state of the biological treatment link in real time through a big data platform, obtaining treatment parameter data, and feeding back and adjusting microbial inoculation parameters based on the treatment parameter data. Through the scheme of the embodiment of the invention, the excrement cleaning and transporting vehicle can be intelligently scheduled through the Internet of Things and the big data platform, and the microbial inoculation program of the pretreatment station is linked, so that the subsequent biological treatment efficiency is guaranteed.

The invention belongs to the field of biological agriculture fermentation, and particularly relates to snakegourd fruit nutrition powder and a preparation method and application thereof.The snakegourd fruit nutrition powder is prepared by conducting primary subzero treatment on snakegourd fruit roots in liquid carbon dioxide, soaking the snakegourd fruit roots subjected to primary subzero treatment in egg yolk hydrolysate for treatment, soaking the snakegourd fruit roots subjected to hydrolysis egg yolk soaking in liquid nitrogen for secondary subzero treatment, and conducting secondary subzero treatment. Soaking the secondarily cryogenic trichosanthes kirilowii maxim roots in liquid carbon dioxide for three-time cryogenic treatment, and then inoculating a fermentation strain for fermentation, enzymolysis and drying. According to the fermentation method, the fermentation uniformity is good, the trichosanthes kirilowii maxim roots do not need to be crushed, and the problem that the trichosanthes kirilowii maxim roots are difficult to ferment due to the fact that raw materials are compact in structure, microbial inoculation and growth are limited, moisture and ventilation are uneven and the like can be solved by conducting subzero treatment on the trichosanthes kirilowii maxim roots three times in cooperation with egg yolk hydrolysate dipping treatment. The fermented feed liquid obtained by fermenting the trichosanthes kirilowii roots can be prepared into trichosanthes kirilowii nutrition powder, and the fermentation residues can be used as a pig feed additive.

The invention discloses a soil preparation method based on molesey coal gangue and application, and relates to the technical field of solid waste resource utilization and soil improvement. The purpose of the invention is to solve the problems of environmental pollution, resource waste, poor adaptability of coal gangue soil preparation, poor improvement effect and incomplete removal of harmful impurities caused by accumulation of the mossy system coal gangue in the prior art. The method comprises the following steps: 1, pretreating the molery-series coal gangue; 2, mixing and improving; 3, microbial inoculation and curing; and 4, planting adaptive adjustment. The prepared soil is applied to mine land reclamation and agricultural planting soil improvement. According to the method, resource utilization of the mossy system coal gangue is effectively achieved, the prepared soil has the advantages of being appropriate in fertility, good in water and fertilizer retention, free of secondary pollution and the like, meanwhile, the soil preparation cost is reduced, remarkable economic, social and environmental benefits are achieved, and the method can be widely applied to the fields of mining area land reclamation, agricultural planting soil improvement and the like.

The present invention provides a method for pretreating straw by using a deep eutectic solvent in combination with a biocatalytic enzymatic method to produce butanol, belonging to the technical field of straw treatment. The present invention provides a method for enzymatic hydrolysis and saccharification of straw, comprising: pretreating the straw with a deep eutectic solvent, and then mixing the pretreated straw and sugar solution, inoculating an enzyme-producing microorganism, and successively performing microbial culture to produce enzymes and enzymatic hydrolysis to obtain a high-concentration sugar solution. In the present invention, DES is used to pretreat the straw to break the natural anti-degradation barrier of lignin and improve the accessibility of cellulose; the enzyme-producing microorganism is inoculated on the DES-pretreated straw to efficiently prepare cellulase, and then enzymatic hydrolysis of the straw is carried out. After DES pretreatment, the degradation rate of lignin in the straw is as high as 23.52%. After enzymatic hydrolysis is completed, the yield of glucose is as high as 88.23%. The obtained high-concentration sugar solution does not contain furan toxic substances and can be used for the preparation of butanol, and will not have an adverse effect on the growth and metabolism of the butanol-producing microbial preparation.

The invention provides a compound self-disinfection inoculation device with multiple inoculation ends, which relates to the technical field of microbial inoculation and comprises an inoculation needle cylinder, an ultraviolet disinfection component arranged in the inoculation needle cylinder and an alcohol disinfection component communicated with the ultraviolet disinfection component, a conveying tube is arranged in the inoculation needle cylinder; two ends of the inoculating needle cylinder are respectively provided with a connecting end and a needle outlet end; the alcohol disinfection component is arranged in the connecting end, a button is arranged on one side of the connecting end, the working face on one side of the button is connected with a connecting rod, the side, close to the ultraviolet disinfection component, of the button is connected with an extension rod, and one end of the extension rod is provided with a preset contact slope; a pushing part is arranged in the ultraviolet disinfection part; a cutting part is arranged on the working surface of the top end of the pushing part; through efficient sterilization of ultraviolet rays and rapid disinfection of alcohol, a compound disinfection system is formed, and the risk of cross infection in the inoculation process is reduced.

The invention discloses a preparation method for producing a biological bacterial fertilizer from lincomycin waste mushroom dregs, and relates to the technical field of fertilizer preparation, and the preparation method comprises the following steps: pretreatment of lincomycin waste mushroom dregs, microbial inoculation, strain fermentation, trace element addition and liquid fertilizer finished product detection. The method has the advantages that the waste lincomycin residues are alternately soaked with diluted hydrochloric acid and a sodium hydroxide solution, various impurities on the surfaces and in the waste lincomycin residues can be fully dissolved, alkaline harmful substances and pollutants combined with metal ions can be decomposed under the acidic condition, and the waste lincomycin residues can be recycled under the alkaline condition. The micro-bubbles can decompose acidic residual antibiotics and organic substances, in the continuous aeration process, on one hand, the micro-bubbles can scour the surfaces of mushroom dregs to enable impurities to fall off and be dissolved more easily, on the other hand, the content of dissolved oxygen in liquid is increased, favorable conditions are created for follow-up microorganism survival, and meanwhile oxidative decomposition of harmful substances is facilitated.

The utility model discloses a mixed microorganism inoculation fermentation device of intelligent control bacteria, including the fermentation cylinder of setting, install motor on the fermentation cylinder, motor output end is connected with first rotating shaft, the eccentric wheel is installed to first rotating shaft outside, the inside setting of fermentation cylinder has the guide rod, the mobile board is arranged to guide rod outside, the rack is installed to mobile board side end, the gear is arranged to rack side end, the second rotating shaft is passed through and is connected to the inside of gear, the screw piece is arranged to second rotating shaft outside. This mixed microorganism inoculation fermentation device of intelligent control bacteria, through the first rotating shaft outside first mixing piece, mixes microorganism, makes different bacteria evenly distribute, promotes the material exchange and information transmission between microorganism, the screw piece of second rotating shaft outside rotates, has reduced the problem that the mixing efficiency is not high because of single stirring, has further improved the evenness of microorganism mixing, has improved the growth speed and yield of overall flora.

This invention relates to the field of food biotechnology and fermentation microbiology, specifically a method for obtaining lactic acid bacteria (LAB) isolates as starter culture candidates. The method is carried out by preparing a wheat-based fermentation substrate and adding gelatin protein as a nitrogen source to form a fermentation medium of wheat, gelatin, and fructose. Indigenous microorganisms in the substrate are inoculated into the fermentation medium and incubated under conditions that support the growth of lactic acid bacteria until lactic acid bacteria isolates are obtained. Fermentation is carried out at a temperature of 30–37°C for 12–72 hours, with a gelatin concentration of 10% (w / v) and a wheat to water ratio of 1:1 to 1:5 (w / v). The fermentation medium, a combination of wheat, gelatin, and fructose, functions as a selective medium for growing and selecting lactic acid bacteria before further isolation on standard microbiological media.This method produces lactic acid bacteria isolates that can be used as starter culture candidates in food fermentation processes.

The invention discloses a layered gel culture technology constructed by using environmental extracts, and belongs to the field of environmental microorganism culture. According to the technology, an environmental sample is separated into a particle phase and a liquid phase through water extraction, the particle phase and the liquid phase are respectively prepared into a lower-layer solid-phase matrix and an upper-layer gel phase with gel (preferably agar), a vertical nutrition / signal slow-release gradient is formed in a culture dish, and particle attachment sites are provided; and inoculating the environmental microorganisms on the upper layer or the surface for culture. Compared with nutrient agar or extremely simple water agar, the method has the advantages that more bacterial colonies and higher distinguishable form number can be obtained under the repeatable condition, and the community diversity and the OTU / ASV overlapping degree with an original sample are improved; rare / slow-growing groups can be stably separated, and subsequent 16S / metagenome analysis and agar block bacteriostasis primary screening are facilitated. Meanwhile, the invention provides a corresponding culture medium composition and a kit / prefabricated layered culture dish. According to the scheme, operation is easy and convenient, parameters can be standardized, and the method is suitable for isolated culture and ecological function research of environmental samples such as soil, sediments and low-solid water samples.

The invention discloses a method suitable for manufacturing a microbial specimen by sealing UV glue, and belongs to the technical field of microbial specimen manufacturing, microorganisms are inoculated to a plate culture medium for culture, then a cultured microbial plate is sterilized and dried, the preheated UV glue is poured into the dried microbial plate, and the microbial specimen is obtained. And carrying out auxiliary defoaming by using a hot air gun or a syringe needle or a vacuum pump, and finally, irradiating for 5 minutes or 8 minutes by using a 36W ultraviolet lamp, so as to obtain the microbial specimen. The method is mainly used for keeping morphological characteristics such as bacterial colony shape and color of microorganisms to the greatest extent after a series of specimen manufacturing process steps are carried out on a cultured microorganism plate with relatively obvious bacterial colony morphological characteristics, the processing steps are simple, convenient and safe, the prepared specimen is stable, and the method is suitable for large-scale popularization and application. The exhibition period is longer, and the exhibition effect is better.

The invention discloses a real silk / regenerated bamboo joint fiber interwoven functional fabric and a preparation method thereof, and belongs to the technical field of textiles. The invention relates to a real silk / regenerated bamboo joint fiber interwoven functional fabric and provides regenerated bamboo joint fibers and real silk. Real silk and regenerated bamboo joint fibers are blended into yarn, the yarn is soaked in warm water to remove surface impurities, microorganisms are inoculated into a culture medium containing the yarn for fermentation culture, and the yarn subjected to microbiological treatment is obtained; soaking the yarn subjected to microbiological treatment in a solution containing an antioxidant, enabling the antioxidant to be uniformly distributed on the surface of the yarn through an adsorption effect, and drying to obtain the pretreated yarn; and weaving the obtained pretreated yarns into the real silk / regenerated bamboo joint fiber interwoven functional fabric. According to the preparation method disclosed by the invention, the real silk / regenerated bamboo joint fiber interwoven functional fabric has the softness of real silk and the moisture absorption, air permeability and antibacterial property of regenerated bamboo joint fibers, and meanwhile, the production cost is reduced.

The utility model belongs to the technical field of biological evaluation of medical instruments, and particularly relates to a wound biological membrane culture system and a dynamic in-vitro wound model for antibacterial activity evaluation, which comprise a culture medium dripping system, a reactor and a waste liquid collecting bottle which are sequentially connected, according to the utility model, the glass slide and the in-vitro pigskin tissue or the glass slide and the in-vitro pigskin tissue, the test dressing and the balancing weight are sequentially arranged in the test bin of the reactor from bottom to top. The in-vitro pigskin tissue is used for wound surface construction and microbial inoculation, continuous nutrition supply is provided, the in-vitro pigskin tissue in-vitro biological membrane preparation device can be used for preparing a wound surface biological membrane, planktonic bacteria infection or biological membrane infection of the wound surface can be simulated, the in-vitro pigskin tissue in-vitro biological membrane preparation device is closer to the actual clinical condition, and the antibacterial activity of the antibacterial wound surface dressing can be predicted more accurately.

The invention discloses tea leaf fermentation liquor rich in functional components and a preparation method of the tea leaf fermentation liquor, and relates to the technical field of deep processing of tea leaves. The preparation method comprises the following steps: mixing tea leaves with water, and performing enzymolysis to obtain tea leaf zymolyte; culturing the microbial strain; performing microbial inoculation on the tea leaf zymolyte to obtain tea leaf fermentation liquor rich in theanine, theanine and gamma-aminobutyric acid; according to the invention, high-value utilization of the trimmed tea leaves can be realized; after tender shoots and tender leaves of tea trees are picked, most of the tea leaves are trimmed and treated as waste materials, the trimmed tea leaves are converted into high-value tea leaf fermentation liquor through the method, the utilization rate of tea leaf resources is increased, and greater economic benefits are brought to producers. The pruned tea leaves are converted into the tea leaf fermentation liquor rich in functional components such as tea pigment, theanine and gamma-aminobutyric acid, high practicability is achieved, and the requirements of the market for natural and functional products can be met.

This invention relates to the field of river water body treatment and maintenance technology, and in particular to a river water body treatment and maintenance device and method, comprising a garbage collection unit, a garbage storage unit, a microbial inoculation unit, and a drive unit. The garbage collection unit is located at the front end of the maintenance device, the garbage storage unit is located behind the garbage collection unit, and the microbial inoculation unit is located on one side of the garbage storage unit. The drive motor of the drive unit is located at the end of the maintenance device, and the airbag of the drive unit is located at the bottom of the maintenance device. This invention employs the above-mentioned river water body treatment and maintenance device and method, which can automatically collect and store floating garbage on the surface of the river water, and then treat and maintain the river water by inoculating a compound microbial agent.

A method of producing single cell protein SCP or biomass comprises culturing microbial inoculum in a growth medium comprising dairy-derived whey. The method may comprise (i) inoculating growth medium

The application discloses a method for co-production of cellulase and bacterial protein based on an integrated microorganism-fiber microcarrier-surfactant suspension system, and belongs to the technical field of cellulase and bacterial protein production. First, the microorganism is inoculated on a culture medium to obtain a seed culture solution, then fiber microcarriers are prepared through micro-bubble continuous flotation, and then the fiber microcarriers are hydrolyzed by acid to form a fiber microcarrier hydrolysis mixed solution, which is dispersed in the microorganism and surfactant to prepare an integrated microorganism-fiber microcarrier-surfactant suspension system, microcarrier suspension fermentation is carried out, and after centrifugation and drying, the supernatant is cellulase, and the solid is bacterial protein. The method can effectively improve the yield of cellulase and bacterial protein, realize partial replacement of exogenous cellulase in the process of lignocellulose saccharification, and thus effectively reduce the preparation cost of lignocellulose saccharification.

The utility model discloses a microorganism inoculation device applied to plant leaves, which comprises a plurality of fitting protection pieces, round bulges are arranged in the fitting protection pieces, accommodating cavities are formed in the bottoms of the round bulges, and disinfection cotton balls and target fungus cakes are sequentially arranged in the accommodating cavities from top to bottom. And two mounting grooves are formed in the bottom of each attaching protection piece, and high-temperature-resistant adhesives are arranged in the two mounting grooves. The utility model mainly has the following advantages: 1, the requirement on the inoculation environment is not too high, and the inoculation can be carried out under the conditions of high temperature and high humidity; 2, the inoculation method realizes the effects of wind resistance, high temperature resistance and rain resistance; 3, one leaf can realize inoculation of different strains or a plurality of fungus cakes of the same strain; 4, only the positions for inoculating the fungus cakes need to be covered with plastic, so that the fungus cakes are light-permeable and breathable, and field planting and disease attack are facilitated; and 5, the inoculation method is simple, and a plurality of operation steps can be completed indoors.

The utility model relates to the technical field of inoculant inoculation, in particular to an inoculant inoculation device for a potted plant biocontrol test, which comprises a base and an inoculant box fixedly mounted on the base, the supporting frame is fixedly installed on the base, a lead screw is rotationally installed on the supporting frame, and a hand wheel is fixedly installed at the top end of the lead screw; the lifting plate is installed on the supporting frame in a sliding mode, and a threaded through hole in threaded connection with the lead screw is formed in the lifting plate; the grooving mechanism is installed at the front end of the lifting plate, the grooving mechanism comprises an arc-shaped pressing plate, an arc-shaped groove is formed in the arc-shaped pressing plate, and scraper knife plates inclined oppositely are installed on the two sides of the arc-shaped groove; and the injection cylinder is fixedly installed on the lifting plate, and a soil returning mechanism is installed on the injection cylinder. The inoculant inoculation device for the potted plant biocontrol test has the advantages that inoculant inoculation is in place, and the test accuracy can be improved conveniently.

This invention belongs to the field of *Coptis chinensis* cultivation technology, specifically relating to a cultivation method for increasing the berberine content in *Coptis chinensis*, comprising the following steps: seedling cultivation, transplanting, microbial inoculation, light management, fertilization management, staged drought stress induction, water management, and harvesting; the microbial inoculation involves simultaneously applying a compound microbial agent containing *Bacillus amyloliquefaciens*, arbuscular mycorrhizal fungi, and plant rhizosphere growth-promoting bacteria at the time of transplanting; the light management involves shading combined with LED supplemental lighting; the staged drought stress induction involves controlling the relative soil moisture content to 45%-55% for 7-10 days after transplanting, 60-75 days after transplanting. This invention, through the synergistic effect of microbial inoculation, light quality regulation, and drought stress induction, can significantly increase the berberine content in the roots of *Coptis chinensis* by more than 120% compared to conventional cultivation methods, and is standardized, green, and sustainable, suitable for large-scale cultivation and promotion.

The application discloses a kind of plant synergistic microbial power generation methods, including preparation membrane electrode, preparation plant material, electrode and plant tissue coupling, reference electrode connection, circuit connection, microbial inoculation, enhance microbial activity and fuel cell start-up etc., the application gets rid of the dependence on water environment, make the operation of plant power generation greatly reduce the influence of environmental factors, power output is more stable, thereby improve power generation efficiency and reliability.Secondly, the application expands the range of coupled plants.In addition, the application innovatively introduces microporous filter membrane between membrane electrode and plant tissue, optimizes the contact and material exchange between electrode and plant tissue, which helps to improve the performance of electrode and the power generation efficiency of fuel cell.