Protease composite enzyme and application thereof

Abstract

The invention relates to a protease composite enzyme and application thereof. A preparation method comprises the following steps of: inoculating microbes into a culture medium, placing the culture medium on a shaker at the temperature of between 25 and 40 DEG C and the rotating speed of 120 to 200 revolutions per minute to culture the microbes for 24 to 96 hours, performing enzyme or ultrasonic wall breakage or non wall breakage treatment, filtering bacteria, adding ammonium sulfate into the culture medium to settle protein, centrifuging the solution, adding Tris-HCl into the solution for dissolution, and freeze-drying the sediment after dialysis; or inoculating microbes into the culture medium, fermenting the culture medium for 23 to 72 hours at the temperature of 30 DEG C, culturing the microbes for 24 to 72 hours at the stirring speed of 120 to 600rpm, stopping fermentation, and filtering or centrifuging bacteria. The composite enzyme can be directly acted on various wool spinning fabrics and saves chemical reagent pretreatment and oxidation pretreatment in the conventional treatment process; the application method is simple and suitable for industrialized production; and the felting resistance and mechanical strength of the treated wool or wool fabrics are improved, and the felting rate of the treated wool fabric is less than 5 percent.

Description

technical field [0001] The invention belongs to the field of compound enzyme and its application, in particular to a protease compound enzyme and its application. Background technique [0002] Wool fabric is a high-grade product that is popular among people. It has advantages that other fiber fabrics cannot match, and its inherent properties make wool fabric a high-grade textile. Hand feel and dyeing performance are important characteristics of wool. However, the outer layer of wool has a dense and hydrophobic scale layer that is difficult to handle, which affects many indicators such as wool feel and dyeing and coloring. People have developed various processes to remove the scale layer for a long time. In the existing factory treatment process, chemical means are the main method, such as oxidizing the wool surface with chlorine water to improve the dyeing affinity or softening agent to improve the feel and characteristics. This treatment is a surface treatment because the ...

AI Technical Summary

Problems solved by technology

At present, the development and application of wool fabric treatment process is mainly based on protease. Ordinary protease cannot treat the disulfide bonds rich in the outer scale layer of wool fiber, so redox pretreatment is necessary.

However, after chemical treatment, protease re-treatment of wool leads to a sharp decrease in wool strength. The main mechanism for this is that the hydrolysis of wool by protease is a relationship of "internal and external competition". Enzymes not only act on the surface of wool, but also penetrate into it. Into the interior of the wool fiber, the final result is that the strength of the wool fiber is greatly reduced

Due to the special stability of the disulfide bond in the scale layer of the outer layer of wool fiber, the effect of using conventional protease is not obvious, and sometimes it has no effect at all (Fan Zenglu et al., "Research on the Mechanism of Protease on Wool", 2002, "Acta Textile Science 》), so at present, the enzymatic treatment of woolen fabrics at home and abroad often adopts chemical pretreatment, which not only fails to achieve the purpose of not using chemical reagents, but also destroys the internal structure of woolen fabrics, and its actual application effect is still not ideal

In the fifth issue of "Jiangsu Silk" in 2006, the article "Application of protease in wool textile fiber modification" pointed out that pretreatment (oxidation or reduction) is a necessary step to modify wool textile with protease, and no pretreatment has been found so far. Protease Achieving Anti-felting Effect of Wool Fabric

People have also developed a resin finishing process to improve fiber strength, but this process causes serious environmental pollution and has not been widely used in practice.

Jason Patrick McDevitt disclosed in the patent US006051033A that the strength of wool is reduced by protease treatment, and then the peptide bond broken between the connecting proteins is treated with transglutaminase, thereby improving the protein fiber strength, but this process increases the processing flow and improves the Processing costs, the market prospect is not great, so there is no large-scale promotion and application at present

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