42 results about "Microbial contaminants" patented technology

The invention relates to the technical field of meat product processing, and discloses a sewage purification treatment method for meat product processing, which comprises the following steps: S1, pretreatment, S2, adjustment of water quality and water quantity, setting of an adjusting tank, adjustment of water quality and water quantity of sewage, realization of homogeneous and uniform treatment of sewage, and provision of buffer capability for organic load; s3, biological treatment, S4, advanced treatment, S5, sludge treatment and S6, monitoring and evaluation, the water quality and the water quantity of sewage in a regulating tank are balanced and regulated, stability is kept, growth and reproduction of microorganisms in the biological treatment stage are facilitated, and the sewage treatment efficiency is improved; after biological treatment, suspended solids, colloidal substances and microbial pollutants in the sewage are further removed through coagulating sedimentation, filtration and disinfection measures, and the effluent quality is improved; and the sludge generated in the sewage treatment process is subjected to resource utilization, so that the pollution to the environment is reduced to a great extent.

The invention discloses a food safety knowledge graph construction method and system based on a neural network, and relates to the technical field of food safety. Food characteristic information data, equipment state information data and external environment state information data are respectively obtained, an equipment surface pollution degree coefficient Xnd and a food surface pollution degree coefficient Xsp of each monitoring time period are calculated and obtained, correlation analysis is carried out, a pollution path correlation index Zwr is calculated, whether a food pollution path comes from processing equipment or not is judged, and if yes, the food pollution path is judged to come from the processing equipment; an atlas construction instruction is sent out; a food safety knowledge graph is constructed by combining a time sequence neural network algorithm, production line equipment which does not meet safety processing requirements is identified through target word retrieval and threshold comparison, and cleaning and disinfection instructions are generated to guarantee food safety.

Compositions, testing chambers and methods for testing a fuel sample for microbial contamination (including fuels treated with a biocide) are provided, which comprise: a quantity of hydrocarbon fuel; a microbial contamination wherein the microbial contamination further comprises nucleic acid in the form of both DNA, RNA or a combination thereof, and an analyzing solution; wherein the analyzing solution comprises at least six (6) primer pairs for amplification of at least one target locus, wherein at least one primer of each pair of primers is labeled with a fluorescent dye and wherein at least one of the primer pair binds to the nucleic acid of the microbial contamination.

Compositions, testing chambers and methods for testing a fuel sample for microbial contamination (including fuels treated with a biocide) are provided, which comprise: a quantity of hydrocarbon fuel; a microbial contamination wherein the microbial contamination further comprises nucleic acid in the form of both DNA, RNA or a combination thereof, and an analyzing solution; wherein the analyzing solution comprises at least six (6) primer pairs for amplification of at least one target locus, wherein at least one primer of each pair of primers is labeled with a fluorescent dye and wherein at least one of the primer pair binds to the nucleic acid of the microbial contamination.

A composition (100) for inhibiting growth of microbial contamination is disclosed The composition includes a plurality of volatile antimicrobial components (110) including at least one of potassium sorbate and sorbic acid. The composition further includes a plurality of non-volatile antimicrobial components (120) including natamycin. A weight ratio of the plurality of volatile antimicrobial components to the plurality of non-volatile antimicrobial components is in the range of 10:2 to 10:1. The composition is placed at a distance from a target surface to inhibit microbial growth on the target surface by releasing the plurality of volatile antimicrobial components and the plurality of non-volatile antimicrobial components into a surrounding air. The target surface is exposed to microbial contaminants. moisture and humidity.

Provided are compositions and methods useful to the determination of whether a microbial contaminant is present in a biological therapeutic production process. Specifically, an artificial positive amplification control plasmid and unique quantitative PCR detection probe are provided, which enables the rapid and real-time detection of a false positive result.

In a controlled environment (1), the invention proposes sealing adjacent drive modules (5) of a transport device (4) of the controlled environment (1) relative to one another in a sealing region (12) such that particles and / or contaminants, in particular microbiological contaminants, do not enter the controlled environment (1) from the outside, each drive module (5) being configured to levitate (6) at least one transport unit (7), in particular a mover (8), and a coupling side (9) of the drive module (5) being very largely covered by a sealing element (10) (Fig. 1).

Methods of preparing a sample for an assay for rapid quantification of a biologic contaminant. The method includes adding the sample to a vessel and inserting the vessel into a centrifuge. The method further includes centrifuging the sample to yield a first supernatant and a first pellet, discarding the first supernatant, and dissolving the first pellet in a first volume of a buffer to yield a first suspension volume. The method further includes centrifuging the first suspension volume to yield a second supernatant and a second pellet, discarding the second supernatant, and dissolving the second pellet in a second volume of the buffer to yield a final sample suspension. The method also includes adding the final sample suspension to an assay for rapid quantification for microbial contaminants.

Liquid, air, or surface treatment / sterilization / disinfection using pulsed light to eliminate, reduce, degrade, render inert, or nullify physical, chemical, biological, microbiological, or non-microbiological contaminants is disclosed herein. The system also includes at least one power supply powering the light source of the reactor, an ionized wire wrapped outside the light source of the reactor, at least one digital multiplexer connected to the at least one power supply, and a controller to control the at least one digital multiplexer to power the light source to strobe the pulsed light at a predetermined fluence, a predetermined pulsed width, and a predetermined frequency or spectrum to eliminate, reduce, degrade, render inert, or nullify the physical, chemical, biological, microbiological, or non-microbiological contaminants in a liquid flow, air, or surface at a molecular level.

Process for detecting gram-negative bacteria and / or fungi contaminants in a detergent comprising gram-positive bacteria comprising the subsequent steps of: Providing a detergent comprising intentionally added spores of Bacillus sp; wherein the amount of spores is from 1x102 to 1x1014 CFU / g of detergent; and Adding a sample of the detergent to a liquid growth medium, wherein the medium comprises an effective amount of gram-positive bacteria selective growth inhibitor, and wherein the medium comprises an effective amount of disinfectant neutralizer to provide a liquid growth mixture; and Incubating the liquid growth mixture for from 6 hours to 48 hours at a temperature of from 18 to 46 degrees Celsius; and Diluting the growth mixture by from 3- to 100-fold to provide a liquid growth mixture dilution; and Lysing cells present in the liquid growth mixture dilution; and Applying an ATP-dependent bioluminescence assay to determine the amount of gram-negative and / or fungi contaminants in the detergent provided at step a.

The invention provides a method for fabricating high-flow ceramic water filters with antimicrobial properties. The process involves preparing a ceramic suspension using mining waste, red clay, limestone, cassava starch, and a dispersing agent. The suspension is cast into dome-shaped metal molds, heated to gelatinize the starch, and thermally treated to create a porous ceramic matrix with about 57% apparent porosity and 4 MPa flexural strength. The porous filter is coated with a metal-doped ferrite oxide (e.g., Cu0.7Co0.3Fe2O4) to impart antimicrobial properties while maintaining high flow rates between 12 to 24 liters per hour. The filters effectively reduce turbidity to 0.14–4 NTU, maintain pH levels of 6.9–8.8, and eliminate microbial contaminants, including Escherichia coli and fecal coliforms. This cost effective method utilizes locally available raw materials, is scalable for industrial production, and is applicable for domestic and industrial water purification systems.

Current methods for detection of microbial contaminants on surfaces use swabbing / wiping to extract microbes for analysis. This removes easily transferable microbes but fails to extract microbes living in biofilms, which reduces sensitivity and may mask the true degree of contamination. The current disclosure provides an enzyme cocktail that disrupts the biofilm and improves the extraction of live microbes for analysis. Applicant's enzyme system is particularly useful for the application to a variety of surfaces, but particularly on a variety of food processing surfaces. Utilization of Applicant's enzyme cocktail makes possible the extraction of a representative sample of live microorganisms present on a surface, including film forming microorganisms, without affecting non-film forming microorganisms also present on a surface.

According to the technical scheme, the high-moisture-retention repairing gel application is characterized in that a surface film layer serves as an outermost protective barrier of the application, so that local humidity regulation and control can be guaranteed through high moisture permeability and permeation resistance of the surface film layer; the invasion of external microorganisms, pollutants, dust particles and the like on wound surfaces or sensitive skin is effectively prevented, and sealing support is provided for the internal gel layer; the gel layer is arranged between the membrane layer and the supporting layer, bears various bioactive components, forms a wet-imitating environment, is in direct contact with the skin during use, quickly releases moisturizing and repairing factors and adjusts the pH and moisture of a microenvironment, and can be attached to the surface of the skin to form a tight and soft contact interface due to the good touch feeling and rheological characteristics of the gel layer; and the substrate supporting layer is used as a bottom carrier to provide structural supporting tension buffering and overall strength enhancing effects on the gel layer, and meanwhile, the application stability is improved, so that a user can conveniently wear the gel on multiple parts and the dynamic skin surface for a long time without easily falling off and being damaged.

The invention discloses a method for evaluating self-cleaning and degerming functions of a washing machine, which comprises the following steps: (1) preparing microbial pollutants and carriers thereof: selecting an aluminum sheet, and uniformly coating the surface of the aluminum sheet with the microbial pollutants to obtain a microbial pollutant carrier; the microbial pollutant carriers are fixed to the center of the bottom of an inner barrel of the washing machine and evenly fixed to the inner wall of the inner barrel at equal intervals, and at least four microbial pollutant carriers are fixed to the inner wall of the inner barrel; starting a self-cleaning program, recovering each microbial pollutant carrier after the self-cleaning program is operated, and measuring the number of bacterial colonies; (3) positive control group test: immobilizing a microbial pollutant carrier in the same way as that of the test group; starting a conventional washing program except for the self-cleaning program, and after the conventional washing program is operated, recycling the microbial pollutant carrier according to a test group method for determining the colony count; and (4) calculating the self-cleaning degerming rate.

The present application relates to the technical field of mouse breeding equipment, and discloses a water replenishing device for an experimental mouse breeding box, including a breeding box, a water storage tank fixedly installed on the top of the breeding box, two through openings on the top of the breeding box, the water storage tank located between the two through openings, a fixed box fixedly installed on the inner wall of the bottom of the breeding box, a rod hole on the top of the fixed box, a feeding mechanism arranged above the fixed box, a sensor mechanism arranged between the fixed box and the breeding box, and a pumping mechanism arranged between the water storage tank and the breeding box. The present application has the following advantages and effects: by arranging the sensor mechanism, the purpose of automatically adding water to the water tank through the downpipe can be achieved, by arranging the filtering mechanism, the organic matter and microbial pollutants in the water can be filtered through the filter element, the purpose of avoiding causing mice to get sick after long-term drinking can be achieved, and then the purpose of avoiding affecting the mouse growth experiment can be achieved.

Compositions, testing chambers and methods for testing a fuel sample for microbial contamination (including fuels treated with a biocide) are provided, which comprise: a quantity of hydrocarbon fuel; a microbial contamination wherein the microbial contamination further comprises nucleic acid in the form of both DNA, RNA or a combination thereof, and an analyzing solution; wherein the analyzing solution comprises at least six (6) primer pairs for amplification of at least one target locus, wherein at least one primer of each pair of primers is labeled with a fluorescent dye and wherein at least one of the primer pair binds to the nucleic acid of the microbial contamination.

A portable greywater test kit (10), consisting of: a power source (11); at least one ONPG roll (16) configured to react with the greywater during discharge, producing color changes to detect bacterial contamination; a servo motor (14) that positions and rotates the roller to gradually release the ONPG roller towards the wastewater; one or more sensors (12) that analyze the intensity and hue of the color change that occurs on the ONPG roll after reaction with microbial impurities; a Raspberry Pi Pico (15) which processes the data received from the color recognition sensor to determine the level of contamination using predefined instructions; at least one OLED display (13) that shows users real-time contamination data by indicating different contamination states, such as harmless, moderate or high contamination, based on the color intensity detected by the sensor; one or more LED indicators (17) provide the user with immediate visual warnings in green, yellow and red during the testing of water quality based on low, medium and high levels of pollution; A tear-off mechanism (19) allows the user to remove and dispose of the used ONPG section at the push of a button; At least one button (18) restarts the test process after the removal of the used ONPG roll in the next operating cycle.

Provided are methods for rapid detection of microbial contaminants in fresh foods, involving obtaining a product sample (preferably at or near the time of harvest of the product), applying, prior to any chilling of the sample or the product, an amount of a enrichment medium sufficient to cover the sample surfaces to provide a non-chilled sample for incubation, incubating the non-chilled sample for a time period and at a temperature sufficient to allow for amplification and enrichment of a non-cold-shocked target microbe, followed by recovering all or a portion of the enrichment media, concentrating the target microbes from the recovered enrichment medium, and detecting the concentrated target microbes.

The present disclosure in one aspect provides a sterile packaging container comprising a container body with a cross-sectional shape that is constant along the majority of the longitudinal axis, a cover and a closure assembly that inhibits the passage of microbial contaminants. The container is configured such that the interior of the container can be sterilized. The sterile packaging container described herein allows one to manufacture a sterile packaging tube exercising the smallest possible volume.

The embodiments disclose an apparatus for testing organic plant materials for microbial contamination including machine-readable QR code identifiers associated with multiple facility testing locations, samples from each location are treated with microorganism-specific growth stimulator solutions and incubated locally in a portable incubator to accelerate microbial growth, a plurality of sensors and a camera, wirelessly coupled to a remote server, capture environmental data and images of the incubated samples, an artificial intelligence (AI) engine on the server analyzes the images and sensor data, correlates them to respective QR code identifiers, and compares them against a pattern recognition database to identify microbial contaminants, a mobile application displays AI-generated contamination reports, remediation recommendations, and a facility heat map identifying locations of recurrent infestations, a machine learning system continuously updates remediation determinations based on sensor feedback, an AI-enabled contamination prediction module models the spread of contamination and generates preventative recommendations to aid in eradication.

Personal equipment having a radiation source or an array of radiation sources for creating an aura of radiation in the vicinity of the user. The created aura is oriented in space, such as to be safe for the user but harmful to microbiological contaminants present in the air in the vicinity of the user and bombarding the user in routine situations.

A method for reducing contamination in agricultural / fishery products, comprising: determining whether the contamination is microbiological and / or chemical, and if so; placing the contaminated agricultural / fishery product inside containers made of a material resistant to high temperatures, high vacuum, positive pressure, and allowing the circulation of gaseous fluids; subject the agricultural / fishery product, inside sterilization equipment, to vaporization with an aqueous solution containing 300 to 5000 ppm of food grade hydrogen peroxide, to obtain an agricultural / fishery product with microbiological contamination reduced by 5 logarithms, and / or a chemical contamination reduced from 95. 0 a 99.9% Agricultural / fishery products decontaminated by the method of the present invention, which has a microbiological contamination reduced in 5 logarithms, and / or a chemical contamination reduced from 95.0 to 99.9%.

The handheld water purification device features an outer body (101) that encases an inner assembly, including an inner body (102), a rechargeable battery (105), and a printed circuit board (106). The device operates by purifying water through a combined process involving a UV lamp (103) and silver electrodes (104). When the device is submerged in a container of water, the UV lamp (103) emits ultraviolet light, while the silver electrodes (104) release silver ions, effectively neutralizing a broad spectrum of microbial contaminants, including gram-positive and gram-negative bacteria. The printed circuit board (106), equipped with a voltage regulator (106a) and a timing controller (106b), precisely manages the operation, ensuring efficient water purification. The device is powered by a rechargeable battery (105), and includes protective caps (108) and a universal charger (109). Compact and portable, this device provides a reliable solution for safe drinking water.

The utility model particularly relates to a filter element and an air purification device. The filter element comprises an inner layer assembly, a filter element body and an outer layer assembly. The filter element comprises a body, a plurality of dividing strips and a plurality of filter particles, the interior of the body is divided into a plurality of cavities by the dividing strips, and the body is of an annular structure; a plurality of filtering particles are arranged in each cavity; the inner-layer assembly, the filter element and the outer-layer assembly are sequentially arranged in a sleeving manner from inside to outside. According to the filter element and the air purification device, the filter element is arranged between the inner-layer assembly and the outer-layer assembly, a more compact multi-layer design is achieved, the AOP solid alkali particles are arranged in the body, and peculiar smells in air and microbial pollutants such as bacteria and viruses can be efficiently removed; according to the air purifier, the interior of the body is divided into the multiple cavities through the multiple dividing strips, and then the body is arranged on the outer side of the inner layer assembly in a surrounding mode, so that air passing through the filter element is evenly purified, and the air purification effect is improved.

Provided is a proficiency testing material, and in more particular aspects, a cannabis proficiency testing material comprising: a plant substrate; and at least one chemical and / or microbial contaminant, wherein the at least one chemical and / or microbial contaminant is chemically or physically associated with the plant substrate. Methods of preparing the proficiency testing material and kits including the same are also provided. 20 26 20 48 88 24 J un 2 02 6 A B S T R A C T 2 0 2 6 2 0 4 8 8 8 2 4 J u n 2 0 2 6

Provided is a disinfectant composition for reducing microbial contaminants on direct and indirect food contact surfaces. In particular, the composition demonstrates a synergistic disinfection mechanism by combining the diffusion mechanism of a booster additive and a peroxy acid. Also provided is a method of reducing microbial contaminants of a surface of a substrate through the deposition of the composition onto a substrate. Finally, provided is a method of disinfecting bottles in cold aseptic filling applications.

<pre>The invention relates to a method for manufacturing a dietary supplement based on beef squid and Ganoderma lucidum mushrooms intended to enhance aphrodisiac properties. The process begins by cleaning, cutting, and grinding the beef squid to obtain a uniform texture. These squid are then blended with powdered Ganoderma lucidum mushrooms in a ratio of 1:1000 (weight / weight). Functional ingredients, such as sea salt, spices, and dextrose, are added to improve preservation, flavor, and texture. Sodium phosphate is also incorporated to optimize water retention. The mixture undergoes gentle pasteurization to eliminate microbial contaminants while preserving the bioactive properties of the ingredients. Finally, the product is homogenized in an industrial blender to ensure a uniform texture and good stability. This dietary supplement supports blood circulation, hormonal balance, and aphrodisiac properties.< / pre>

The invention belongs to the technical field of biological medicines, and particularly discloses an acinetobacter baumannii capsular polysaccharide-protein conjugate as well as a preparation method and application thereof. The acinetobacter baumannii capsular polysaccharide repetitive unit is used as a target product, and the specific compound is synthesized by 12 steps of reaction, including adding corresponding catalysts into different compounds in a specific solvent for reaction, and performing separation, purification and other operations. And carrying out deprotection and other treatments on the product to finally obtain a product q. The acinetobacter baumannii capsular polysaccharide-protein conjugates r and s are obtained by synthesizing pentasaccharides, then covalently linking the synthesized pentasaccharides with bovine serum albumin (BSA) and diphtheria toxoid (DT) through 3, 4-diethoxy-3-cyclobutene-1, 2-diketone respectively, and obtaining the acinetobacter baumannii capsular polysaccharide-protein conjugates r and s. According to the developed chemical total synthesis route, the oligosaccharide fragments chemically synthesized through the technical route have the advantages of being high in purity and clear in chemical structure and do not contain potential microbial pollutants, and meanwhile oligosaccharide fragments of different lengths can be controllably synthesized.

A method of preparing a sample for rapid quantification of the determination of biological contaminants. The method includes adding a sample to a vessel and inserting the vessel into a centrifuge. The method further includes centrifuging the sample to produce a first supernatant and a first precipitate, discarding the first supernatant, and dissolving the first precipitate in a first volume of buffer to produce a first suspension volume. The method further includes centrifuging the first suspension volume to produce a second supernatant and a second precipitate, discarding the second supernatant, and dissolving the second precipitate in a second volume of buffer to produce a final sample suspension. The method further includes adding the final sample suspension to an assay for rapid quantification of microbial contaminants.