28 results about "Microbial contaminants" patented technology

Compositions, testing chambers and methods for testing a fuel sample for microbial contamination (including fuels treated with a biocide) are provided, which comprise: a quantity of hydrocarbon fuel; a microbial contamination wherein the microbial contamination further comprises nucleic acid in the form of both DNA, RNA or a combination thereof, and an analyzing solution; wherein the analyzing solution comprises at least six (6) primer pairs for amplification of at least one target locus, wherein at least one primer of each pair of primers is labeled with a fluorescent dye and wherein at least one of the primer pair binds to the nucleic acid of the microbial contamination.

Compositions, testing chambers and methods for testing a fuel sample for microbial contamination (including fuels treated with a biocide) are provided, which comprise: a quantity of hydrocarbon fuel; a microbial contamination wherein the microbial contamination further comprises nucleic acid in the form of both DNA, RNA or a combination thereof, and an analyzing solution; wherein the analyzing solution comprises at least six (6) primer pairs for amplification of at least one target locus, wherein at least one primer of each pair of primers is labeled with a fluorescent dye and wherein at least one of the primer pair binds to the nucleic acid of the microbial contamination.

A composition (100) for inhibiting growth of microbial contamination is disclosed The composition includes a plurality of volatile antimicrobial components (110) including at least one of potassium sorbate and sorbic acid. The composition further includes a plurality of non-volatile antimicrobial components (120) including natamycin. A weight ratio of the plurality of volatile antimicrobial components to the plurality of non-volatile antimicrobial components is in the range of 10:2 to 10:1. The composition is placed at a distance from a target surface to inhibit microbial growth on the target surface by releasing the plurality of volatile antimicrobial components and the plurality of non-volatile antimicrobial components into a surrounding air. The target surface is exposed to microbial contaminants. moisture and humidity.

Provided are compositions and methods useful to the determination of whether a microbial contaminant is present in a biological therapeutic production process. Specifically, an artificial positive amplification control plasmid and unique quantitative PCR detection probe are provided, which enables the rapid and real-time detection of a false positive result.

In a controlled environment (1), the invention proposes sealing adjacent drive modules (5) of a transport device (4) of the controlled environment (1) relative to one another in a sealing region (12) such that particles and / or contaminants, in particular microbiological contaminants, do not enter the controlled environment (1) from the outside, each drive module (5) being configured to levitate (6) at least one transport unit (7), in particular a mover (8), and a coupling side (9) of the drive module (5) being very largely covered by a sealing element (10) (Fig. 1).

Process for detecting gram-negative bacteria and / or fungi contaminants in a detergent comprising gram-positive bacteria comprising the subsequent steps of: Providing a detergent comprising intentionally added spores of Bacillus sp; wherein the amount of spores is from 1x102 to 1x1014 CFU / g of detergent; and Adding a sample of the detergent to a liquid growth medium, wherein the medium comprises an effective amount of gram-positive bacteria selective growth inhibitor, and wherein the medium comprises an effective amount of disinfectant neutralizer to provide a liquid growth mixture; and Incubating the liquid growth mixture for from 6 hours to 48 hours at a temperature of from 18 to 46 degrees Celsius; and Diluting the growth mixture by from 3- to 100-fold to provide a liquid growth mixture dilution; and Lysing cells present in the liquid growth mixture dilution; and Applying an ATP-dependent bioluminescence assay to determine the amount of gram-negative and / or fungi contaminants in the detergent provided at step a.

The invention provides a method for fabricating high-flow ceramic water filters with antimicrobial properties. The process involves preparing a ceramic suspension using mining waste, red clay, limestone, cassava starch, and a dispersing agent. The suspension is cast into dome-shaped metal molds, heated to gelatinize the starch, and thermally treated to create a porous ceramic matrix with about 57% apparent porosity and 4 MPa flexural strength. The porous filter is coated with a metal-doped ferrite oxide (e.g., Cu0.7Co0.3Fe2O4) to impart antimicrobial properties while maintaining high flow rates between 12 to 24 liters per hour. The filters effectively reduce turbidity to 0.14–4 NTU, maintain pH levels of 6.9–8.8, and eliminate microbial contaminants, including Escherichia coli and fecal coliforms. This cost effective method utilizes locally available raw materials, is scalable for industrial production, and is applicable for domestic and industrial water purification systems.

Current methods for detection of microbial contaminants on surfaces use swabbing / wiping to extract microbes for analysis. This removes easily transferable microbes but fails to extract microbes living in biofilms, which reduces sensitivity and may mask the true degree of contamination. The current disclosure provides an enzyme cocktail that disrupts the biofilm and improves the extraction of live microbes for analysis. Applicant's enzyme system is particularly useful for the application to a variety of surfaces, but particularly on a variety of food processing surfaces. Utilization of Applicant's enzyme cocktail makes possible the extraction of a representative sample of live microorganisms present on a surface, including film forming microorganisms, without affecting non-film forming microorganisms also present on a surface.

The invention discloses a method for evaluating self-cleaning and degerming functions of a washing machine, which comprises the following steps: (1) preparing microbial pollutants and carriers thereof: selecting an aluminum sheet, and uniformly coating the surface of the aluminum sheet with the microbial pollutants to obtain a microbial pollutant carrier; the microbial pollutant carriers are fixed to the center of the bottom of an inner barrel of the washing machine and evenly fixed to the inner wall of the inner barrel at equal intervals, and at least four microbial pollutant carriers are fixed to the inner wall of the inner barrel; starting a self-cleaning program, recovering each microbial pollutant carrier after the self-cleaning program is operated, and measuring the number of bacterial colonies; (3) positive control group test: immobilizing a microbial pollutant carrier in the same way as that of the test group; starting a conventional washing program except for the self-cleaning program, and after the conventional washing program is operated, recycling the microbial pollutant carrier according to a test group method for determining the colony count; and (4) calculating the self-cleaning degerming rate.

Compositions, testing chambers and methods for testing a fuel sample for microbial contamination (including fuels treated with a biocide) are provided, which comprise: a quantity of hydrocarbon fuel; a microbial contamination wherein the microbial contamination further comprises nucleic acid in the form of both DNA, RNA or a combination thereof, and an analyzing solution; wherein the analyzing solution comprises at least six (6) primer pairs for amplification of at least one target locus, wherein at least one primer of each pair of primers is labeled with a fluorescent dye and wherein at least one of the primer pair binds to the nucleic acid of the microbial contamination.

A portable greywater test kit (10), consisting of: a power source (11); at least one ONPG roll (16) configured to react with the greywater during discharge, producing color changes to detect bacterial contamination; a servo motor (14) that positions and rotates the roller to gradually release the ONPG roller towards the wastewater; one or more sensors (12) that analyze the intensity and hue of the color change that occurs on the ONPG roll after reaction with microbial impurities; a Raspberry Pi Pico (15) which processes the data received from the color recognition sensor to determine the level of contamination using predefined instructions; at least one OLED display (13) that shows users real-time contamination data by indicating different contamination states, such as harmless, moderate or high contamination, based on the color intensity detected by the sensor; one or more LED indicators (17) provide the user with immediate visual warnings in green, yellow and red during the testing of water quality based on low, medium and high levels of pollution; A tear-off mechanism (19) allows the user to remove and dispose of the used ONPG section at the push of a button; At least one button (18) restarts the test process after the removal of the used ONPG roll in the next operating cycle.

Provided are methods for rapid detection of microbial contaminants in fresh foods, involving obtaining a product sample (preferably at or near the time of harvest of the product), applying, prior to any chilling of the sample or the product, an amount of a enrichment medium sufficient to cover the sample surfaces to provide a non-chilled sample for incubation, incubating the non-chilled sample for a time period and at a temperature sufficient to allow for amplification and enrichment of a non-cold-shocked target microbe, followed by recovering all or a portion of the enrichment media, concentrating the target microbes from the recovered enrichment medium, and detecting the concentrated target microbes.

The present disclosure in one aspect provides a sterile packaging container comprising a container body with a cross-sectional shape that is constant along the majority of the longitudinal axis, a cover and a closure assembly that inhibits the passage of microbial contaminants. The container is configured such that the interior of the container can be sterilized. The sterile packaging container described herein allows one to manufacture a sterile packaging tube exercising the smallest possible volume.

The embodiments disclose an apparatus for testing organic plant materials for microbial contamination including machine-readable QR code identifiers associated with multiple facility testing locations, samples from each location are treated with microorganism-specific growth stimulator solutions and incubated locally in a portable incubator to accelerate microbial growth, a plurality of sensors and a camera, wirelessly coupled to a remote server, capture environmental data and images of the incubated samples, an artificial intelligence (AI) engine on the server analyzes the images and sensor data, correlates them to respective QR code identifiers, and compares them against a pattern recognition database to identify microbial contaminants, a mobile application displays AI-generated contamination reports, remediation recommendations, and a facility heat map identifying locations of recurrent infestations, a machine learning system continuously updates remediation determinations based on sensor feedback, an AI-enabled contamination prediction module models the spread of contamination and generates preventative recommendations to aid in eradication.

The handheld water purification device features an outer body (101) that encases an inner assembly, including an inner body (102), a rechargeable battery (105), and a printed circuit board (106). The device operates by purifying water through a combined process involving a UV lamp (103) and silver electrodes (104). When the device is submerged in a container of water, the UV lamp (103) emits ultraviolet light, while the silver electrodes (104) release silver ions, effectively neutralizing a broad spectrum of microbial contaminants, including gram-positive and gram-negative bacteria. The printed circuit board (106), equipped with a voltage regulator (106a) and a timing controller (106b), precisely manages the operation, ensuring efficient water purification. The device is powered by a rechargeable battery (105), and includes protective caps (108) and a universal charger (109). Compact and portable, this device provides a reliable solution for safe drinking water.

Provided is a proficiency testing material, and in more particular aspects, a cannabis proficiency testing material comprising: a plant substrate; and at least one chemical and / or microbial contaminant, wherein the at least one chemical and / or microbial contaminant is chemically or physically associated with the plant substrate. Methods of preparing the proficiency testing material and kits including the same are also provided. 20 26 20 48 88 24 J un 2 02 6 A B S T R A C T 2 0 2 6 2 0 4 8 8 8 2 4 J u n 2 0 2 6

Provided is a disinfectant composition for reducing microbial contaminants on direct and indirect food contact surfaces. In particular, the composition demonstrates a synergistic disinfection mechanism by combining the diffusion mechanism of a booster additive and a peroxy acid. Also provided is a method of reducing microbial contaminants of a surface of a substrate through the deposition of the composition onto a substrate. Finally, provided is a method of disinfecting bottles in cold aseptic filling applications.

<pre>The invention relates to a method for manufacturing a dietary supplement based on beef squid and Ganoderma lucidum mushrooms intended to enhance aphrodisiac properties. The process begins by cleaning, cutting, and grinding the beef squid to obtain a uniform texture. These squid are then blended with powdered Ganoderma lucidum mushrooms in a ratio of 1:1000 (weight / weight). Functional ingredients, such as sea salt, spices, and dextrose, are added to improve preservation, flavor, and texture. Sodium phosphate is also incorporated to optimize water retention. The mixture undergoes gentle pasteurization to eliminate microbial contaminants while preserving the bioactive properties of the ingredients. Finally, the product is homogenized in an industrial blender to ensure a uniform texture and good stability. This dietary supplement supports blood circulation, hormonal balance, and aphrodisiac properties.< / pre>

The invention belongs to the technical field of biological medicines, and particularly discloses an acinetobacter baumannii capsular polysaccharide-protein conjugate as well as a preparation method and application thereof. The acinetobacter baumannii capsular polysaccharide repetitive unit is used as a target product, and the specific compound is synthesized by 12 steps of reaction, including adding corresponding catalysts into different compounds in a specific solvent for reaction, and performing separation, purification and other operations. And carrying out deprotection and other treatments on the product to finally obtain a product q. The acinetobacter baumannii capsular polysaccharide-protein conjugates r and s are obtained by synthesizing pentasaccharides, then covalently linking the synthesized pentasaccharides with bovine serum albumin (BSA) and diphtheria toxoid (DT) through 3, 4-diethoxy-3-cyclobutene-1, 2-diketone respectively, and obtaining the acinetobacter baumannii capsular polysaccharide-protein conjugates r and s. According to the developed chemical total synthesis route, the oligosaccharide fragments chemically synthesized through the technical route have the advantages of being high in purity and clear in chemical structure and do not contain potential microbial pollutants, and meanwhile oligosaccharide fragments of different lengths can be controllably synthesized.

A method of preparing a sample for rapid quantification of the determination of biological contaminants. The method includes adding a sample to a vessel and inserting the vessel into a centrifuge. The method further includes centrifuging the sample to produce a first supernatant and a first precipitate, discarding the first supernatant, and dissolving the first precipitate in a first volume of buffer to produce a first suspension volume. The method further includes centrifuging the first suspension volume to produce a second supernatant and a second precipitate, discarding the second supernatant, and dissolving the second precipitate in a second volume of buffer to produce a final sample suspension. The method further includes adding the final sample suspension to an assay for rapid quantification of microbial contaminants.

The invention discloses a soybean cleaning and drying all-in-one machine for soybean powder production, the soybean cleaning and drying all-in-one machine comprises a rack, a brushing mechanism, a flushing mechanism, a driving mechanism and a drying mechanism, the rack is provided with a dust falling bin, a water bin and a connecting pipe, the brushing mechanism is installed above the dust falling bin and comprises a first outer cylinder, a floating brush and a first roller, and mechanical bristles are matched with high-pressure gas to clean the dust falling bin; the washing mechanism is installed above the water bin and comprises a second outer cylinder, a second roller, a steam brush and a second spray head, worm eggs, mildew spots and microbial pollutants attached to the surfaces of the soybeans are efficiently killed and removed through the steam effect, and the drying mechanism comprises a conveyor and a fan. According to the soybean cleaning and instant drying device, classified fine treatment of soybean impurities is achieved, the cleanliness is remarkably improved, and meanwhile the water content of the soybeans is effectively controlled through combination of steam cleaning and instant drying.

Compositions, testing chambers and methods for testing a fuel sample for microbial contamination (including fuels treated with a biocide) are provided, which comprise: a quantity of hydrocarbon fuel; a microbial contamination wherein the microbial contamination further comprises nucleic acid in the form of both DNA, RNA or a combination thereof, and an analyzing solution; wherein the analyzing solution comprises at least six (6) primer pairs for amplification of at least one target locus, wherein at least one primer of each pair of primers is labeled with a fluorescent dye and wherein at least one of the primer pair binds to the nucleic acid of the microbial contamination.

The utility model discloses a sterilization mechanism for food additives. The sterilization mechanism comprises a base, the sterilization box is arranged on the base; the heating device is arranged on the inner wall of the sterilization box; a plurality of ultraviolet sterilization lamps are uniformly distributed at the upper end in the sterilization box; the stirring assembly comprises a driving motor, a rotary drum and a plurality of stirring rods; the plurality of through holes are uniformly distributed in the upper end of the side wall of the rotary drum and are positioned at the same horizontal height; the utility model relates to the technical field of food additive processing, the stirring component and the feeding component form internal circulation, so that the heating device can provide uniform heat, active ingredients in food additives are effectively protected, and the inactivation of the food additives due to local high temperature is avoided; meanwhile, the materials are exposed to an ultraviolet irradiation area for multiple times in the circulation process, the defect that ultraviolet penetrating power is insufficient is overcome, the deep material sterilization effect is remarkably improved, and microbial pollutants in the raw materials can be more comprehensively killed under the dual effects.

The embodiments disclose an apparatus for testing organic plant materials for microbial contamination including machine-readable QR code identifiers associated with multiple facility testing locations, samples from each location are treated with microorganism-specific growth stimulator solutions and incubated locally in a portable incubator to accelerate microbial growth, a plurality of sensors and a camera, wirelessly coupled to a remote server, capture environmental data and images of the incubated samples, an artificial intelligence (AI) engine on the server analyzes the images and sensor data, correlates them to respective QR code identifiers, and compares them against a pattern recognition database to identify microbial contaminants, a mobile application displays AI-generated contamination reports, remediation recommendations, and a facility heat map identifying locations of recurrent infestations, a machine learning system continuously updates remediation determinations based on sensor feedback, an AI-enabled contamination prediction module models the spread of contamination and generates preventative recommendations to aid in eradication.

This invention discloses a method for filtering liquid materials based on a piezoelectric-driven membrane separation system, comprising the following steps: constructing a piezoelectric-driven membrane separation system, the system including a piezoelectric microporous atomizing plate and a microfiltration membrane, the microfiltration membrane being tightly attached to the surface of the piezoelectric microporous atomizing plate; adding the liquid material to be filtered into the liquid inlet space above the microfiltration membrane; applying a high-frequency alternating voltage to the piezoelectric element to drive the liquid material through the microfiltration membrane for filtration, thereby obtaining purified liquid. This invention employs a membrane-piezoelectric microporous atomizing plate tightly coupled configuration, which can effectively trap suspended dispersed phases and microbial contaminants in the liquid material, reduce the risk of atomizing plate cone pore clogging, and significantly improve flux retention and operational stability in protein and polysaccharide systems.

Provided are compositions and methods useful to the determination of whether a microbial contaminant is present in a biological therapeutic production process. Specifically, an artificial positive amplification control plasmid and unique quantitative PCR detection probe are provided, which enables the rapid and real-time detection of a false positive result.

The utility model discloses an air filtering structure of an air compressor, which relates to the field of air filtering of air compressors and comprises an outer end partition frame, a limiting rod is mounted at one end of the outer end partition frame, a nanofiber primary effect layer is mounted in the outer end partition frame, a nanometer contact peptide bacteria layer is mounted on the inner wall of the nanofiber primary effect layer, and a nanometer contact peptide bacteria layer is mounted on the inner wall of the nanometer contact peptide bacteria layer. Medical-grade filter paper is installed on the inner wall of the nanometer contact peptide bacteria layer, and an activated carbon combination layer is installed on the inner wall of the medical-grade filter paper. Through the arrangement of the nanofiber primary effect layer, the nanometer contact peptide bacteria layer, the medical-grade filter paper and the activated carbon combined layer, large-particle pollutants can be pre-filtered, the service life of the high-efficiency filter layer is prolonged, meanwhile, microbial pollutants such as bacteria and viruses in the high-efficiency filter layer can be effectively removed, the purification degree of air and water is improved, and the service life of the high-efficiency filter layer is prolonged. And the activated carbon can adsorb peculiar smell and remove harmful gas, can assist in removing suspended particles in air or water to a certain extent, and provides a filtering effect.

The invention relates to the field of water treatment, and discloses a nanogel filter material and a preparation method and application thereof.The nanogel filter material comprises a component A and a component B. The component A comprises agarose gel, calcium peroxide nano-particles, copper-based carbon quantum dots and an MOF material are distributed in the agarose gel, the copper-based carbon quantum dots and the MOF material are compounded together, the surfaces of the copper-based carbon quantum dots are provided with negative potential, and the surface of the MOF material is provided with negative potential. The surface of the MOF material has positive potential, the MOF material and the MOF material are compounded together through the acting force between the positive potential and the negative potential, and the component B is an acidic solution. When the filtering material provided by the invention is used for filtering, the inactivation and purification of the ultra-microbial pollutants in a sample can be realized through manual injection, the cracking of the pollutants is realized through high-activity ROS broad-spectrum attack active sites in the filtering process, the cracked ultra-microorganisms are automatically discharged, a filter membrane is not easy to block, the filter membrane does not need to be frequently replaced, and the cost is low. And the used filter material does not have the problem of secondary pollution.