The invention provides a dialysis concentrate and a preparation and quality detection method thereof, and belongs to the technical field of dialyzates, the dialysis concentrate comprises lactate, a penetrant and a buffer agent; the lactate comprises sodium chloride, calcium chloride and magnesium chloride; the penetrant comprises glucose, icodextrin, cyclodextrin, modified starch, polyol, fructose, protein and amino sugar; the penetrant further comprises a regulator; the buffering agent is at least one of sodium lactate, bicarbonate, pyruvate and citrate. The dialysis concentrate is formed by mixing the lactate, the penetrant and the buffering agent, the sodium chloride, the calcium chloride and the magnesium chloride are the ion content of the dialysate, the added glucose, icodextrin and cyclodextrin penetrant can contribute to the permeation of the dialysate, and the penetrant hardly interferes with the sodium lactate, so that the dialysis concentrate has the advantages that the dialysis concentrate is safe and reliable. And the content accuracy of the mixed sodium lactate is ensured.