4 results about "Icodextrin" patented technology

The invention provides a preparation method of high-yield icodextrin by combining an ultrasonic field with a single enzyme system. The preparation method comprises the following steps: S1, enzymolysis; s2, performing primary ultrafiltration; s3, ultrasonic hydrolysis; s4, carrying out secondary ultrafiltration; and S5, spray drying. According to the method, firstly, the proportion of dextrin with the molecular weight smaller than 1638 Da in the enzymatic hydrolysate is controlled to be not larger than 7%, the working procedures of recycling macromolecular dextrin and conducting ultrasonic hydrolysis are added, and the yield can reach 80-88%. New amylase does not need to be additionally added in ultrasonic hydrolysis, the hydrolysis time is greatly shortened, the pertinence is high, ultrasonic hydrolysis after enzyme deactivation belongs to physical modification, the reaction degree is conveniently controlled, the generation proportion of dextrin with the molecular weight smaller than 1638 Da is accurately controlled, the production cost is low, the preparation efficiency is high, the consistency of a hydrolysis product and an original product is good, and the method is suitable for industrial production. The generation of 5-hydroxymethylfurfural is completely eradicated, so that the peritoneal dialysis can be continuously carried out.

The application belongs to the technical field of surgical anti-adhesion liquids, and particularly relates to an anti-adhesion liquid containing icodextrin for abdominal surgery or laparoscopic surgery and a preparation method thereof. The anti-adhesion liquid is composed of component A and component B, and the component A and the component B are stored separately. The component A is a solution with water as a solvent, and includes 20-100 g / L icodextrin, 0-20 g / L sodium lactate, 0.1-0.6 g / L calcium chloride, 0.03-0.15 g / L magnesium chloride and 0-60 g / L sodium chloride. The component B is a solution with water as a solvent, and includes 0-54 g / L sodium chloride, 10-20 g / L sodium bicarbonate and 0-19 g / L sodium lactate. The volume ratio of the component A to the component B is 1:0.1-0.9. The pH of the component A is 3.0-6.5, and the pH of the component B is 4.5-9.0. The anti-adhesion liquid can clean a wound surface and prevent tissue and organ adhesion.

This invention discloses a method for calculating the branching degree of icodextrin using carbon nuclear magnetic resonance spectroscopy, belonging to the field of pharmaceutical chemical detection technology. The icodextrin sample to be tested is subjected to nuclear magnetic resonance testing to obtain... 13 The C-NMR spectrum is analyzed by integrating the carbon peak at position 1 of the α-1,6-glycosidic bond in the icolodextrin branch and assigning the integral to 1. Then, the carbon peak at position 1 of the α-1,4-glycosidic bond in the icolodextrin main chain is integrated. The ratio of the integral value of the carbon peak at position 1 of the α-1,6-glycosidic bond in the icolodextrin branch to the total integral of the carbon peak at position 1 in the icolodextrin molecule is the branching degree of icolodextrin. This invention provides a direct calculation of the branching degree of icolodextrin without damaging the sample structure. It is simpler, more convenient, more accurate, easier to operate, and faster, making it a suitable method for detecting the branching degree of icolodextrin.

This invention relates to a method for determining the concentration of ectoine in rat plasma and tissues, belonging to the field of bioanalytical technology. The method employs high-performance liquid chromatography-tandem mass spectrometry (LC-MS / MS) to detect the concentration of ectoine in rat plasma and tissue samples. A 5% acetonitrile aqueous solution is used as the tissue homogenate, and 0.1% trifluoroacetic acid-acetonitrile is used as the precipitation solvent. The mobile phase in the liquid chromatography consists of trifluoroacetic acid, ammonium acetate, water, and acetonitrile, with isocratic elution. Tolbutamide is used as an internal standard for ectoine sample determination. The LC-MS / MS method for determining ectoine in biological samples established in this invention is simple to operate, highly sensitive, requires small sample volumes, and provides rapid analysis. It is suitable for pharmacokinetic studies of ectoine in vivo and meets the analytical requirements for biological samples in terms of accuracy, precision, and selectivity, as outlined in the "Technical Guidelines for Non-Clinical Pharmacokinetic Studies of Chemical Drugs."