This invention discloses a method and
system for measuring soil microbial
nitrogen pumps, belonging to the field of soil
biogeochemistry and agricultural
ecological environment monitoring technology. The method involves applying
nitrogen to a soil-
crop system... 15 N-labeled
nitrogen fertilizer, combined with in-situ closed sampling, membrane
sample mass spectrometry (MIMS) detection, and
amino sugar compound specificity. 15 This invention analyzes nitrogen emissions, simultaneously measuring the fluxes and isotopic characteristics of N2 emissions, NH3 volatilization, and N2O emissions, as well as nitrogen uptake by plants,
residual nitrogen in the soil, and nitrogen fixed by microorganisms. Finally, it constructs a microbial nitrogen pump efficiency (MNUE) index to characterize the intensity and efficiency of soil microbial nitrogen pumps. This invention enables comprehensive accounting of multiple nitrogen fluxes under near-real-
field conditions, accurately distinguishing the contributions of
fertilizer-source and soil-source nitrogen to
gaseous nitrogen emissions. It is applicable to anaerobic ecosystems such as flooded paddy fields and wetlands, providing a systematic and operable technical solution for the quantitative study of soil microbial nitrogen pumps.