10 results about "West Nile virus" patented technology

The present invention pertains to compounds that inhibit Flavivirus NS2B-NS3 proteases and are potent and selective inhibitors of the West Nile virus and Zika virus. The compounds of the invention can be used alone or in combination with other agents to treat infections caused by these viruses. Additionally, the invention relates to pharmaceutical compositions containing these compounds.

The present invention relates to compounds for use in the treatment of a disease or condition caused by Zika virus, Dengue virus, West Nile virus, Chikungunya virus, O`nyong`nyong virus, Sindbis virus or SARS-CoV-2 virus.

The application discloses an anti-Western Nile virus neutralizing antibody WN-XH3 and application thereof. The antibody comprises a heavy chain variable region and a light chain variable region, has good binding activity with Western Nile virus E protein, and has strong virus neutralizing activity, thereby laying a foundation for diagnosis and treatment of Western Nile virus infection related diseases, development of a Western Nile virus infection related disease treatment drug and the like, and can be used as a candidate of a clinical anti-Western Nile virus drug, and has a good application prospect.

The invention provides a reagent and a kit for jointly detecting four avian-derived RNA (Ribonucleic Acid) viruses. The reagent comprises an upstream primer, a downstream primer and a probe which are used for detecting H5N1, H7N9, avian paramyxovirus IX type and west nile virus. Through specific primer and probe sequences, H5N1, H7N9, avian paramyxovirus IX type and West Nile virus in a biological product can be detected at the same time only through one-time amplification in a one-tube reaction, and the detection reagent or kit has the advantages of high sensitivity, strong specificity, good repeatability and the like for four avian-derived RNA viruses, and has a wide application prospect. Mutual interference and cross reaction are avoided, and a large number of samples can be rapidly detected.

The application provides a reagent and kit for jointly detecting four kinds of avian RNA viruses. The reagent of the application comprises an upstream primer, a downstream primer and a probe for detecting H5N1, H7N9, avian parainfluenza virus type IX and West Nile virus. Through specific primer and probe sequences, H5N1, H7N9, avian parainfluenza virus type IX and West Nile virus in biological products can be simultaneously detected in one tube reaction through only one amplification, and the detection reagent or kit of the application has the advantages of high sensitivity, strong specificity, good repeatability and the like for the four kinds of avian RNA viruses, and has no interference and no cross reaction among each other, and can rapidly detect a large number of samples.

This application provides a multiplex quantitative PCR detection kit for mosquito-borne viruses and its detection method. The detection kit includes a multiplex PCR reaction solution, a positive control, and a negative control. The multiplex PCR reaction solution includes four specific probes and four pairs of specific primers for detecting dengue virus, Japanese encephalitis virus, Zika virus, and West Nile virus, respectively. The detection method includes sample extraction, preparation of the PCR mixture of reagents, PCR amplification, and fluorescence signal reading. Through the above methods, this application can specifically identify dengue virus, Japanese encephalitis virus, Zika virus, and West Nile virus. By combining a standard curve, it can quantitatively detect dengue virus, Japanese encephalitis virus, Zika virus, and West Nile virus, with high detection efficiency, stable implementation, simple and rapid operation, high detection speed, and high detection accuracy and sensitivity.

This disclosure provides a device and a method for combining a lumbar puncture needle and an ultrasonic density detection circuitry to provide an inexpensive and accurate lumbar puncture locator device. The disclosed device senses different tissue densities with different frequency tones and colored light indicators before needle insertion in the patient's lumbar vertebrae region. This invention allows a doctor, a nurse practitioner or a physician assistant to safely do a lumbar puncture in an office environment without the scheduling of the use of the radiology department. This device will allow a timelier diagnosis of infections (such as Lyme disease, bacterial and viral meningitis, herpes, West Nile virus, syphilis), multiple sclerosis, Alzheimer's dementia, tumors, idiopathic intracranial hypertension, subarachnoid hemorrhage, and others.

The invention relates to a kit capable of simultaneously detecting a West Nile virus, a Zika virus and a yellow fever virus. The kit comprises a primer pair II for detecting the West Nile virus, a primer pair III for detecting the Zika virus and a primer pair IV for detecting the yellow fever virus, wherein primer sequences contained in the primer pair II are respectively as shown in SEQ ID NO: 4 and SEQ ID NO: 5; the primer pair III comprises an upstream primer III-1, an upstream primer III-2 and a downstream primer III, and the sequences of the upstream primer III-1, the upstream primer III-2 and the downstream primer III are respectively shown as SEQ ID NO: 7-9; primer sequences contained in the primer pair IV are respectively shown as SEQ ID NO: 11 and SEQ ID NO: 12. The kit disclosed by the invention can be used for simultaneously performing one-step fluorescent quantitative PCR detection on the West Nile virus, the Zika virus and the yellow fever virus, the sensitivity and the specificity of the kit are high, and stable and effective fluorescent signals can be obtained when the copy number of fragments in a detection sample reaches 250 copies / mL.

The present invention relates to the use of compounds in the treatment of a viral infection, wherein the virus is selected from the Orthomyxoviridae family or wherein the virus is West Nile virus.

This invention discloses a crRNA target and a CRISPR-Cas13a system for detecting West Nile virus. The West Nile virus target sequence is shown in SEQ ID NO.1. The CRISPR-Cas13a system includes Cas13a protein and crRNA, or a complex of the two. The crRNA includes an anchoring sequence for binding to the Cas13a protein and a guide sequence for targeting the West Nile virus target sequence. The West Nile virus target sequence is shown in SEQ ID NO.1 and is located at positions 274-301 of the West Nile virus genome. Experiments demonstrate that the crRNA of this invention can achieve highly sensitive and specific detection of West Nile virus nucleic acid by activating Cas13a, with a sensitivity reaching one copy (1 copy / μL).