33 results about "West Nile virus" patented technology

The invention relates to the technical field of biological detection, in particular to a detection primer for African horse pestivirus and west nile virus and a nucleic acid mass spectrometry detection method. On the basis of an MALDI-TOF nucleic acid mass spectrum detection method, two relatively conservative genes of VP7 and NS2 are selected as target genes for the African horse pestivirus; and for the West Nile virus, two same conservative genes NS2A and NS5 are selected. Based on the target genes, four pairs of amplification primers and four extension primers are designed. By optimizing selection of amplification sites and adding of modified bases, it is ensured that the molecular weight of the extension probe is effectively distinguished within the detection range, so that the multiple detection target is achieved, and meanwhile high accuracy, specificity and sensitivity of detection are ensured. The kit can efficiently, sensitively and accurately identify the infection conditions of the African horse pestivirus and the west nile virus, can be flexibly adjusted and optimized according to actual requirements, and is expected to play an important role in virus detection and differential diagnosis in the future.

The invention belongs to the technical field of virus detection, and discloses a digital PCR (Polymerase Chain Reaction) kit for simultaneously detecting African horse pestivirus, West Nile virus and equine influenza H3N8, and the kit comprises primers and probes as shown in SEQ ID NO.1-SEQ ID NO.21. The invention also discloses a kit for simultaneously detecting African horse pestivirus, West Nile virus and equine influenza H3N8. According to the invention, after a single system of an African horse pestivirus VP7 gene, an African horse pestivirus-NS2 gene, a west nile virus-NS5 gene, a west nile virus-NS2a gene, an equine influenza H3N8-HA gene and an equine influenza H3N8-NA gene is tested, a seven-fold digital PCR (Polymerase Chain Reaction) system containing an internal standard is established; through tests of indexes such as linearity, specificity, minimum detection limit, repeatability, comparison of a single digital PCR system and a multiple digital PCR system, the currently developed digital PCR multiple detection kit completely meets development requirements and is high in specificity and sensitivity, and the minimum detection limit of the digital PCR multiple detection kit can reach 10 Copies / mu L or below.

The present disclosure relates to field of lyophilized / freeze-dried viral vaccine composition and methods for manufacturing and obtaining the vaccine composition comprising live attenuated flavivirus as vaccine antigen selected from a group of yellow fever virus, dengue virus, Japanese encephalitis (JE) virus, Kunjin virus, West Nile (WN) virus, tick-borne encephalitis (TBE) virus, St. Louis encephalitis virus, Murray Valley encephalitis virus, Zika virus vaccine antigen; and stabilizers comprising sugar or sugar alcohol; amino acids; lactalbumin hydrolysate and gelatin. Post reconstitution, the composition preserves the desired characteristics of the virus, including immunogenicity and stability. The composition of the present disclosure can be safely administered subcutaneously or intramuscularly such that the vaccine composition induces complete immune response to yellow fever virus infectious agent and meets the criterion for the seroprotection for the said immunogenic components comprising yellow fever virus.

The invention provides a multiplex fluorescent quantitative PCR (polymerase chain reaction) detection kit for mosquito-borne viruses and a detection method of the multiplex fluorescent quantitative PCR detection kit. The detection kit comprises multiple PCR reaction liquid, positive quality control and negative control, the multiplex PCR reaction liquid comprises four specific probes and four pairs of specific primers which are respectively used for detecting the dengue fever virus, the Japanese B encephalitis virus, the Zika virus and the west nile virus; the detection method comprises the following steps: extracting a to-be-detected sample, preparing a PCR mixed solution of a to-be-detected drug, performing PCR amplification and reading a fluorescence signal. Through the mode, the dengue virus, the Japanese B encephalitis virus, the Zika virus and the west nile virus can be specifically recognized, the dengue virus, the Japanese B encephalitis virus, the Zika virus and the west nile virus can be quantitatively detected by combining the standard curve, the detection efficiency is high, the implementation can be stable, the operation is simple, convenient and rapid, and the detection cost is low. The detection speed is high, and the detection accuracy and sensitivity are relatively high.

The invention discloses a digital PCR (polymerase chain reaction) detection method for west nile virus, and belongs to the technical field of molecular biological detection. According to the invention, a WNV conserved region NS2A sequence is taken as a template, a primer probe is designed, a West Nile Virus WNV detection method with good repeatability, high sensitivity and good specificity is established, and a detection result shows that the method has good specificity and high sensitivity, and can be used for detecting the West Nile Virus WNV. The kit has the advantages that the kit is free of cross reaction on poultry and livestock diseases such as toxoplasmosis (TOX), brucella (BRF), avian influenza virus (AIV), H3N8 equine influenza virus, NiV (Nipah virus), equine influenza virus (EHV) and equine arteritis virus (EAV), the detection sensitivity of the WNV virus can reach 4 copies / mu L, and results show that the established WNV digital PCR method has the characteristics of high efficiency, specificity, sensitivity and the like and can be used for test detection of the West Nile virus.

The invention discloses a quadruple fluorescent quantitative PCR (Polymerase Chain Reaction) primer probe group for rapidly detecting four pathogens of birds and application, and belongs to the technical field of biology. The kit comprises specific primers and probes aiming at coronavirus (gamma, delta), avian influenza virus (H9 subtype), Newcastle disease virus and West Nile virus; the reagent disclosed by the invention is used for detecting four viruses carried by birds, and has the advantages of high specificity, strong sensitivity, short detection time, safety, low cost and the like; the method can realize detection of pathogens carried by birds, and is of great significance to customs frontier defense and quarantine work.

The present disclosure is concerned with substituted tetrahydrobenzothiophene and tetrahydropyridothiophene compounds, pharmaceutical compositions comprising the compounds, and methods of using the compounds in the treatment of viral infections due to a Flavi virus (e.g, yellow fever, Japanese encephalitis, dengue (DENV), West Nile virus (WNV), zika (ZIKV), tick-bome encephalitis virus. Kunjin virus. Murray Valley encephalitis, St Louis encephalitis, Omsk hemorrhagic fever virus, bovine viral diarrhea virus, Hepatitis C virus) or an Alphavirus (e.g., Venezeulan equine encephalitis virus, chikungunya virus (CHIKV), Ross River virus, Mayaro virus, Sindbis virus). This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.

The present application relates to the technical field of biological detection, and in particular to a detection primer and nucleic acid mass spectrometry detection method for African horse sickness virus and West Nile virus. Based on the MALDI-TOF nucleic acid mass spectrometry detection method, the present application selects two relatively conservative genes, VP7 and NS2, as target genes for African horse sickness virus; and for West Nile virus, two equally conservative genes, NS2A and NS5, are selected. Based on these target genes, the present application designs four pairs of amplification primers and four extension primers. Through optimization of the selection of amplification sites and addition of modified bases, the molecular weight of the extension probe is effectively distinguished within the detection range, thereby achieving the goal of multiplex detection, and at the same time ensuring high accuracy, specificity and sensitivity of the detection. The present application can not only efficiently, sensitively and accurately identify the infection of African horse sickness virus and West Nile virus, but also can be flexibly adjusted and optimized according to actual needs, and is expected to play an important role in future virus detection and differential diagnosis.

The present disclosure targets West Nile virus, Respiratory Syncytial Virus, and other disease-causing microbes, including viruses, bacteria, fungi, and parasites. It does this using agents and methods with little toxicity compared to existing therapies.

This invention belongs to the field of virus detection technology and discloses a digital PCR kit for the simultaneous detection of African horse sickness virus (ESV), West Nile virus (WNVI), and equine influenza H3N8. The kit includes primers and probes as shown in SEQ ID NO.1 to SEQ ID NO.21. After testing single-particle systems of the ESV-VP7 gene, ESV-NS2 gene, WNVI-NS5 gene, WNVI-NS2a gene, equine influenza H3N8-HA gene, and equine influenza H3N8-NA gene, a seven-particle digital PCR system including an internal standard was established. After testing linearity, specificity, limit of detection, repeatability, and comparison of single-particle and multiplex digital PCR systems, the developed digital PCR multiplex detection kit fully meets the development requirements, exhibiting high specificity and sensitivity, with limits of detection all below 10 copies / μL.

The present invention pertains to compounds that inhibit Flavivirus NS2B-NS3 proteases and are potent and selective inhibitors of the West Nile virus and Zika virus. The compounds of the invention can be used alone or in combination with other agents to treat infections caused by these viruses. Additionally, the invention relates to pharmaceutical compositions containing these compounds.

The invention discloses a hybridoma cell strain, a West Nile virus monoclonal antibody as well as a preparation method and application of the West Nile virus monoclonal antibody. The West Nile virus monoclonal antibody is secreted by a hybridoma cell strain WN-mAb-12, the hybridoma cell strain is preserved in the China Center for Type Culture Collection (the address is Wuhan University, Wuhan, China), the preservation number is CCTCC NO: C2024123, and the preservation date is April 23, 2024. The monoclonal antibody secreted by the hybridoma cell strain WN-mAb-12 can generate a neutralization effect on the West Nile virus, and has a good neutralization effect. By analyzing the biological characteristics of the West Nile virus, a technical support is laid for researching the protection mechanism of the West Nile virus neutralizing antibody and researching and developing clinical specific medicines in the future.

The present invention relates to compounds for use in the treatment of a disease or condition caused by Zika virus, Dengue virus, West Nile virus, Chikungunya virus, O`nyong`nyong virus, Sindbis virus or SARS-CoV-2 virus.

The application discloses an anti-Western Nile virus neutralizing antibody WN-XH3 and application thereof. The antibody comprises a heavy chain variable region and a light chain variable region, has good binding activity with Western Nile virus E protein, and has strong virus neutralizing activity, thereby laying a foundation for diagnosis and treatment of Western Nile virus infection related diseases, development of a Western Nile virus infection related disease treatment drug and the like, and can be used as a candidate of a clinical anti-Western Nile virus drug, and has a good application prospect.

The invention provides a reagent and a kit for jointly detecting four avian-derived RNA (Ribonucleic Acid) viruses. The reagent comprises an upstream primer, a downstream primer and a probe which are used for detecting H5N1, H7N9, avian paramyxovirus IX type and west nile virus. Through specific primer and probe sequences, H5N1, H7N9, avian paramyxovirus IX type and West Nile virus in a biological product can be detected at the same time only through one-time amplification in a one-tube reaction, and the detection reagent or kit has the advantages of high sensitivity, strong specificity, good repeatability and the like for four avian-derived RNA viruses, and has a wide application prospect. Mutual interference and cross reaction are avoided, and a large number of samples can be rapidly detected.

This invention relates to methods of treating or preventing viral infections caused by flaviviruses, such as dengue virus, yellow fever virus, West Nile virus or Japanese encephalitis virus or infections caused by Chikungunya virus (CHIKV). The methods involve the administration of retinoic acid analogues to subjects who have, are suspected of having a flavivirus infection or infection with CHIKV, or to those who are at risk of becoming infected with a flavivirus or becoming infected with CHIKV.

The application provides a reagent and kit for jointly detecting four kinds of avian RNA viruses. The reagent of the application comprises an upstream primer, a downstream primer and a probe for detecting H5N1, H7N9, avian parainfluenza virus type IX and West Nile virus. Through specific primer and probe sequences, H5N1, H7N9, avian parainfluenza virus type IX and West Nile virus in biological products can be simultaneously detected in one tube reaction through only one amplification, and the detection reagent or kit of the application has the advantages of high sensitivity, strong specificity, good repeatability and the like for the four kinds of avian RNA viruses, and has no interference and no cross reaction among each other, and can rapidly detect a large number of samples.

The present disclosure provides compounds for the treatment of a variety of diseases such as respiratory syncytial virus (RSV), HRV, hMPV, Ebola virus, Zika virus, West Nile virus, Dengue fever, and HCV.

The present invention relates to compounds that are flavivirus NS2B-NS3 protease inhibitors and inhibit flavivirus replication in cells. The compound provided by the invention can be independently used or combined with other medicaments for treating infections caused by flaviviruses, particularly infections caused by dengue fever virus, West Nile virus, Zika virus and Japanese encephalitis virus.

The present invention relates to a flavivirus NS2B-NS3 protease inhibitor, a compound that inhibits the replication of flaviviruses in cells. The compounds of the present invention are useful alone or in combination with other agents for use in the treatment of infectious diseases caused by flaviviruses, particularly dengue virus, West Nile virus, chikungunya virus, Japanese encephalitis virus.

The utility model provides a bird west nile virus and avian influenza virus sampling device, and relates to the field of bird virus detection, the bird west nile virus and avian influenza virus sampling device comprises a sampling rod, sampling cotton heads are arranged at two ends of the sampling rod, the surface of the sampling rod is in sliding connection with a sleeve, the sleeve is sleeved on the outer surface of the sampling cotton head, and the sampling cotton heads are arranged on the sampling rod. A tooth groove is formed in the inner ring of the sleeve, tooth rings are symmetrically and fixedly installed on the outer surface of the sampling rod, and a baffle is fixedly installed on the side, away from the tooth rings, of the sampling rod; according to the bird West Nile virus and avian influenza virus sampling device, the sleeve arranged at one end slides towards the middle part, so that the sampling cotton head can leak out, the position of the sleeve can be limited through the tooth ring and the baffle plate, the sampling rod can be held by hand for sampling, after the sampling is finished, the sleeve is reset, and the end cover is additionally arranged, so that the sampling cotton head can leak out; through the arrangement, the sampling cotton head can be prevented from being polluted, and the other end can be used through repeated actions.

The present invention discloses a group of molecular targets for detecting hemorrhagic fever pathogens and their applications, wherein the hemorrhagic fever pathogens are Marburg virus, Rift Valley fever virus, yellow fever virus, West Nile virus, dengue virus and Zika virus. The present invention provides primers, primer-probe combinations and kits for detecting six hemorrhagic fever pathogens, and adds exogenous internal standards to monitor the nucleic acid extraction process and PCR amplification process to improve the accuracy of the results, optimizes the amplification system to speed up the detection speed, and the detection limit is as low as 400 copies / mL, with high sensitivity, good repeatability and strong specificity, providing a new technical option for the prevention and treatment of hemorrhagic fever.

This application provides a multiplex quantitative PCR detection kit for mosquito-borne viruses and its detection method. The detection kit includes a multiplex PCR reaction solution, a positive control, and a negative control. The multiplex PCR reaction solution includes four specific probes and four pairs of specific primers for detecting dengue virus, Japanese encephalitis virus, Zika virus, and West Nile virus, respectively. The detection method includes sample extraction, preparation of the PCR mixture of reagents, PCR amplification, and fluorescence signal reading. Through the above methods, this application can specifically identify dengue virus, Japanese encephalitis virus, Zika virus, and West Nile virus. By combining a standard curve, it can quantitatively detect dengue virus, Japanese encephalitis virus, Zika virus, and West Nile virus, with high detection efficiency, stable implementation, simple and rapid operation, high detection speed, and high detection accuracy and sensitivity.

This disclosure provides a device and a method for combining a lumbar puncture needle and an ultrasonic density detection circuitry to provide an inexpensive and accurate lumbar puncture locator device. The disclosed device senses different tissue densities with different frequency tones and colored light indicators before needle insertion in the patient's lumbar vertebrae region. This invention allows a doctor, a nurse practitioner or a physician assistant to safely do a lumbar puncture in an office environment without the scheduling of the use of the radiology department. This device will allow a timelier diagnosis of infections (such as Lyme disease, bacterial and viral meningitis, herpes, West Nile virus, syphilis), multiple sclerosis, Alzheimer's dementia, tumors, idiopathic intracranial hypertension, subarachnoid hemorrhage, and others.

Disclosed herein is provided a virus purification and formulation process for purifying a flavivirus represented by one of a Yellow Fever Virus, Japanese Encephalitis virus, Dengue virus, and West Nile virus. The highly purified flavivirus virus product is characterized as having a low level of sucrose without significant virus loss such as that which is typically encountered by prior art virus purification processes. The disclosed process captures and purifies the virus, separating it from the host cell proteins and DNA, and leaving the host cell proteins and DNA behind. The process also can be used to inactivate and / or concentrate the virus sufficiently for use in formulations.

The present invention provides a PEGylated multivalent anti-West Nile virus single-chain antibody, relating to the field of biomedicine. The present invention provides a method for improving the antigen-binding ability of a ScFv single-chain antibody. Using phage library screening technology, an anti-West Nile virus ScFv single-chain antibody with antigen-binding and in vitro neutralizing activity was obtained. The multivalent anti-West Nile virus ScFv single-chain antibody obtained through PEGylation has a lower risk of ADE and higher antigen-binding ability, providing new research directions and tools for studying the mechanisms of West Nile virus-related diseases and developing therapeutic drugs.

The present disclosure is concerned with benzimidazole compounds and methods of using these compounds for the treatment of hepatitis (e.g., hepatitis C), RNA virus infections (e.g., Zika virus, dengue virus, Powassan virus, Chikungunya virus, Enterovirus, respiratory syntactical virus (RSV), Rift Valley fever, Influenza virus, Tacaribe virus, Mayaro virus, West Nile virus, yellow fever virus, and coronavirus), and disorders of uncontrolled cellular proliferation (e.g., cancer). This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.

The invention relates to a kit capable of simultaneously detecting a West Nile virus, a Zika virus and a yellow fever virus. The kit comprises a primer pair II for detecting the West Nile virus, a primer pair III for detecting the Zika virus and a primer pair IV for detecting the yellow fever virus, wherein primer sequences contained in the primer pair II are respectively as shown in SEQ ID NO: 4 and SEQ ID NO: 5; the primer pair III comprises an upstream primer III-1, an upstream primer III-2 and a downstream primer III, and the sequences of the upstream primer III-1, the upstream primer III-2 and the downstream primer III are respectively shown as SEQ ID NO: 7-9; primer sequences contained in the primer pair IV are respectively shown as SEQ ID NO: 11 and SEQ ID NO: 12. The kit disclosed by the invention can be used for simultaneously performing one-step fluorescent quantitative PCR detection on the West Nile virus, the Zika virus and the yellow fever virus, the sensitivity and the specificity of the kit are high, and stable and effective fluorescent signals can be obtained when the copy number of fragments in a detection sample reaches 250 copies / mL.

The present invention is directed to compounds of the formula: [Image disponible dans le document PDF, Image available in the PDF document] , compositions and methods for preventing, treating or curing Hepatitis B (HBV), West Nile virus and flaviviridae infection in human subjects or other animal hosts. The compounds are as also pharmaceutically acceptable, salts, prodrugs, and other derivatives thereof as pharmaceutical compositions and methods for treatment, prevention or eradication of HBV), West Nile virus and flaviviridae infection.

The invention discloses a neutralizing antibody WN-XH3 for resisting a West Nile virus and an application of the neutralizing antibody WN-XH3. The antibody comprises a heavy chain variable region and a light chain variable region, has relatively good binding activity with West Nile virus E protein, also has relatively strong virus neutralizing activity, lays a foundation for diagnosis and treatment of West Nile virus infection related diseases, development of drugs for treating the West Nile virus infection related diseases and the like, and has wide application prospects. The compound can be used as a candidate of clinical anti-West Nile virus drugs, and has a good application prospect.