78 results about "Epidemic encephalitis" patented technology

The invention relates to a composition for killing virus and preventing influenza and a cold prevention plaster prepared by the composition. An aromatic traditional Chinese medicine volatile extract product and fragrant plant essential oil are used as effective components of the plaster; high-molecular colloid, which can be compatible with the effective components and has a stereo network structure, and an excipient are used to wrap the effective components to prepare a microcapsule; the microcapsule is used to coat on a matrix to prepare a tablet; a double faced adhesive tape is used to plaster the tablet at the position close to mouth and nose (such as collar, shoulder, chest and the like) so as to allow the effective components to slowly release at uniform speed. The plaster can be used to kill pathogen and virus in the air and enhance the immunity of upper respiratory tract, has an obvious effect of preventing influenza, and has a certain prevention effect for common cold, hand-foot-mouth disease, varicella, measles, epidemic encephalitis B, epidemic mumps, chronic respiratory tract diseases and the like. The plaster is convenient to use, has fragrant smell and is safe without side-effect.

The invention relates to a mosquito-repelling bactericide. The raw material formula comprises main ingredients, wherein the main ingredients comprise the following components: clove, folium artemisiae argyi, honeysuckle, elsholtzia, radix angelicae, purple perilla, calamus, mint and agastache rugosus. The mosquito-repelling bactericide disclosed by the invention has the beneficial effects that according to the common sense and principle that mosquitoes bite to suck blood and spread diseases, the bactericide has the main effect of repelling mosquitoes, and harmful pathogenic bacteria and bacteria in air can be killed by virtue of the flavor volatilized by the traditional Chinese medicines, so that the family members are prevented from being troubled by the mosquitoes and are even prevented from suffering from various diseases due to mosquito bites, and people are kept away from diseases such as malaria, filariasis, epidemic encephalitis B and dengue fever.

The invention discloses a kit for detecting a plurality of mosquito borne pathogens and a detection method thereof. The plurality of mosquito-borne infectious pathogens are detected by using multiplex polymerase chain reaction (PCR) combined with a liquid chip, and a yellow fever virus (YFV), dengue fever viruses (DV) I-IV type, epidemic encephalitis B (japanese encephalitis) virus (JEV), plasmodium (Plasmodium), (including plasmodium vivax (PV), plasmodium falciparum (PF), plasmodium malariae (PM), plasmodium ovale (PO)), a west nile virus (MNV) and a chikungunya virus (CHIKV) can be detected in parallel one time. The kit has the advantages of being large in flux, high in specificity and sensitivity, stable in result, good in repeatability, simple to operate and fast in detection speed.

The air germicide for antagonizing epidemic encephalitis and influenza viruses is prepared with storax, benzoin, basket kern, capillary Artemisia, eupatorium and other eight kinds of Chinese medicinal materials in certain weight proportion. The preparation process includes the following steps: 1. soaking the materials, decocting and filtering to obtain medicine liquid; 2. concentrating the medicine liquid, cooling, adding alcohol to precipitate, filtering to obtain the filtrate, adding eucalyptus oil in the same weight to the filtrate, reacting and precipitating for 1 day; and 3. adding clove oil in 5 wt%. The present invention has obvious effect of preventing influenza and other upper respiratory tract infection diseases, and is volatized through heating or naturally.

The invention provides a preparation method of hairy holly root mixed fruit tea. The hairy holly root mixed fruit tea utilizes fresh hairy holly roots as a raw material; and the reparation method comprises the following processing steps: carrying out rolling, roasting, partial freeze-drying, circular brewing, ultrasonic extraction, water chestnut treatment, reflux extraction, blending, homogenization, sterilization and so on. The preparation method preserves nutrients in the raw material, so that the processed final product of the fruit tea is rich in nutrition as well as fine and smooth in taste; the fruit tea has the healthcare functions of clearing away heat, detoxifying, eliminate swelling, relieving pain, preventing epidemic encephalitis and so on. Thus, nutritive values and economic values of the hairy holly roots are improved, and a new way for developing and utilizing the hairy holly roots is provided.

The invention discloses an epidemic encephalitis B / forest encephalitis hybrid virus and an application of the virus. The epidemic encephalitis B / forest encephalitis hybrid virus takes an epidemic encephalitis virus attenuated strain as a gene skeleton, and is a recombinant virus obtained by replacing a deoxyribonucleotide fragment of an encoded epidemic encephalitis virus prM-E delta 3 protein in epidemic encephalitis virus genome RNA (Ribonucleic Acid) with a deoxyribonucleotide fragment of an encoded forest encephalitis virus prM-E delta 3 protein in forest encephalitis virus genome RNA. The epidemic encephalitis B / forest encephalitis hybrid virus has the advantages that the virus is high in safety, and strong in stability, and can be induced to generate a good immune response to a forest encephalitis virus envelope protein, and protect an animal from being attacked by the exogenous forest encephalitis virus of lethal dose. The epidemic encephalitis B / forest encephalitis hybrid virus has a good application prospect in preventing and / or treating forest encephalitis virus infection as a vaccine.

The invention relates to element fabric health-improving protective clothing capable of killing and repelling mosquitoes, which belongs to the technical field of the production of healthy textile multifunctional textile fabric clothing. More than 360 types of mosquitoes are recorded in China. The mosquitoes are fond of sucking blood of warm-blooded animals, infect malaria, filariasis, yellow fever, dengue, epidemic encephalitis B and even H1N1 flu and threaten human health. The element fabric health-improving protective clothing, which is produced by performing nanometered disposal on mixed materials of a plurality of trace elements needed by a human body, a broad-spectrum powerful silver antibacterial agent, an anti-mosquito finishing agent SCJ-951, mosquito-killing pyrethrin and the like and adding the mixture into spinning solution of cotton, flax, fur, polypropylene fiber, polyamide fiber, acrylic fiber and the like, kills and repels mosquitoes and improves health.

The invention discloses a breeding method for breeding pigs, and relates to the technical field of pig breeding. In the breeding pig breeding process, the breeding pigs in different phases such as piglet phase, reserve swine phase and sow phase are subjected to immunization by various vaccines in different time stages. According to the breeding method, a normative immunization program is provided, so that swine fever, foot-and-mouth diseases, pseudorabies, porcine reproductive and respiratory syndrome, parvovirus, epidemic diarrhea, contagious gastroenteritis porcine rotavirus, epidemic encephalitis B, haemophilus parasuis, porcine circovirus and the like which are common in the piglets, the reserve swines and the sows can be effectively prevented.

The invention discloses a pidemic encephalitis B / dengue chimeric virus with an epidemic encephalitis B virus attenuated strain as a gene framework and application of the epidemic encephalitis B / dengue chimeric virus. The invention provides a deoxyribonucleic acid (DNA) molecule, and the DNA molecule is recombinant DNA which is obtained by replacing an encoding gene of protein A in complementary DNA (cDNA) corresponding to genome ribonucleic acid (RNA) of an epidemic encephalitis B virus by using an encoding gene of protein B; the protein A consists of an amino acid sequence shown as a sequence 1 in a sequence table; and the protein B consists of an amino acid sequence shown as a sequence 2 in the sequence table. The pidemic encephalitis B / dengue chimeric virus with the epidemic encephalitis B virus attenuated strain as the gene framework is a recombinant virus containing the DNA molecule. The pidemic encephalitis B / dengue type II chimeric virus also has a good application prospect when taken as a dengue vaccine and used for preventing dengue virus infection.

The invention discloses mosquito repellent. The mosquito repellent is made of raw materials including, by weight, 5-15 parts of flos caryophylli, 10-15 parts of folium artemisiae argyi, 8-20 parts of flos lonicerae, 5-7 parts of herba moslae, 10-12 parts of herba menthae, 6-9 parts of agastache rugosa and 10-30 parts of realgar. The mosquito repellent has the advantages that the mosquito repellent is prepared from purely natural Chinese herbal medicines and chemical products, mosquitoes mainly can be expelled according to mosquito biting and blood sucking and disease spreading common sense and principles, harmful germs, bacteria and the like in air can be killed by odor volatilized from the traditional Chinese medicines, accordingly, family members can be guaranteed against being disturbed by the mosquitoes and can be prevented from suffering from diversified diseases due to mosquito biting, effects of keeping the family members away from diseases such as malaria, filariasis, epidemic encephalitis and dengue fever can be realized, methods for manufacturing the mosquito repellent are simple, raw materials for the mosquito repellent are easily available, and the like.

The invention provides a method for preparing a swine epidemic encephalitis B inactivated vaccine. According to the method, a Chinese hamster lung cell adaptive virus screened by bacteriophage plaque cloning and purification is capable of generating cytopathic effect, the virulence is stabilized at 107.0-108.0TCID50 / mL, and the immunogenicity is stable; the swine epidemic encephalitis B virus is multiplied by utilizing the hamster pulmonary cell system has good virus adaptability and high virus virulence of prepared vaccine. According to the method, a virus concentrating process technology which sequentially performs separating and concentrating is adopted, and virus solution and cell debris can be separated by utilizing a hollow fibrous membrane clarifying and purifying system, so that the problems that the membrane is easily blocked, is sensitive to shearing force and easily aggregated when virus particles are concentrated can be solved, side effects of an inactivated vaccine finished product can be effectively reduced, and the safety of the inactivated vaccine can be improved. Binary ethyleneimine is used as an inactivator instead of formaldehyde and has small destruction on virus antigen, so that the completeness of effective immunogen in the antigen can be guaranteed.

The invention relates to a combined vaccine prepared from epidemic encephalitis B viral vaccines and enterovirus 71 (EV71) viral vaccines and a preparation method thereof. An epidemic encephalitis B viral stock solution prepared from the epidemic encephalitis B virus through culture, harvest, inactivation, ultrafilter concentration and purification and an EV71 viral stock solution prepared from the EV71 virus through culture, harvest, inactivation, ultrafilter concentration and purification are prepared according to a certain proportion to form the combined vaccine. The combined vaccine can be used for simultaneously preventing and treating the hand-foot-and-mouth disease suffered by infants and children caused by the epidemic encephalitis B and the EV71 virus.

The invention relates to a sweet wormwood ointment with anti-mosquito and anti-malaria effects. The ointment is mainly prepared from the following raw materials: nepeta oil, menthol, dementholized peppermint oil, sweet wormwood oil, blumea oil, lemongrass oil, eugenol, artesunate, alpha-bisabolol and an ointment excipient, prevent mosquito bite well, and further prevent rapid spread of diseases which mainly take mosquitos as media such as malaria, dengue fever, filariasis or epidemic encephalitis B. The sweet wormwood ointment further has effect of rapidly relieving itching and removing swelling on itching and swelling which are caused by mosquito bite.

The invention discloses a gene chip and kit for detecting swine epidemic encephalitis B viruses (SEEBVs) and / or swine fever viruses (SFVs). The gene chip comprises a solid phase carrier and a probe immobilized on the solid phase carrier, wherein the probe comprises any one or two oligonucleotide fragments shown in SEQ ID NO:1 or 2 and / or any one or two oligonucleotide fragments shown in SEQ ID NO:3 or 4. The kit comprises the gene chip and reagents for amplifying genes of the SEEBVs and / or the SFVs. The gene chip and the detection kit can be used for accurately and effectively detecting the SEEBVs and the SFVs, have strong specificity, high sensitivity and good application prospects, consume short time and are fast in detection.

The invention relates to a preparation method of a vaccine for epidemic encephalitis B, which comprises the processes of cell culture, virus propagation and vaccine preparation. After cells overgrow with a single layer, a seed culture of viruses and a culture solution are inoculated, and the use quantity of the seed culture of the viruses is that every 100cm<2> of cells is inoculated with 1ml of seed culture of the viruses. The culture solution of the viruses mainly comprises 90-95% of MEM culture solution, 3-5% of calf serum and 7.5% of sodium bicarbonate solution the pH of which is regulated to 7.2-7.6. The vaccine is prepared by adding human serum albumin with the final concentration of 0.3% after being clarified and filtered. The invention adopts an SA14-14-2 attenuated strain to produce an encephalitis purified vaccine. Compared with a vaccine produced by a P3 strain, the attenuated strain is safer and more effective. The invention has the advantages that: (1) the attenuated strain is used for preparing the purified vaccine, which has good safety; (2) the inactivation effect of inactivating the viruses by using beta-propiolactone is good, and an inactivator is naturally degraded and has no residue; and (3) the column chromatography purification property is mild, and the injury to the viruses is small.

The invention discloses a method for detecting real-time fluorescence quota PCR of epidemic encephalitis B virus and a kit. A primer and a TaqMan probe is designed in accordance with the conserved region of the epidemic encephalitis B virus E gene, which is used for quantitatively detecting nucleic acid copy number of the epidemic encephalitis B virus in samples. Particularly, the forward primer of the primer has a nucleotide sequence of SEQ ID NO: 1 in a sequence table, the reverse primer of the primer has a nucleotide sequence of SEQ ID NO: 2 in the sequence table, and TaqMan probe has a nucleotide sequence in SEQ ID NO: 3 in the sequence table. The invention has larger valuable significance in the field of controlling the quality of biological products related to the human and animal sources and inspecting and quarantining the imported and exported animals, ensures the quality of the related biological products and controls the spread of the epidemic encephalitis B and the medication safety of people. The detection method and the kit of the invention can be used for inspecting the epidemic encephalitis B virus on cerebrospinal fluid samples of the clinical patients, miniature pig brain tissue samples and the biological products, and have wide application prospect.

The invention relates to animal vaccines, and specifically discloses an antigen of an epidemic encephalitis live vaccine and a preparation method and application thereof. Vero cells are adopted to carry out roller bottle culture on epidemic encephalitis virus attenuated strains (SA14-14-2 strains); virus culture maintenance solution which comprises components such as 4-hydroxyethylpiperazine ethane sulfonic acid, arginine monohydrochloride and PEG2000 is used; the produced antigen has the advantages of being small in batch difference, good in immunogenicity and high in virus titer; and the epidemic encephalitis live vaccine prepared through the antigen is capable of effectively preventing the epidemic encephalitis virus infection of pigs.

The invention provides an epidemic meningitis polysaccharide combined vaccine, and aims to prepare a freeze-dried ACYW135 meningococcal polysaccharide vaccine by the process of removing endotoxins by the ultracentrifugation method so as to ensure that the content of endotoxins present in ACYW135 group meningococcal polysaccharide stock solution is far lower than that before the centrifugation. The vaccine provided by the invention has the advantages of ensuring that only one injection is enough to prevent a patient from being infected with epidemic meningitis disease, achieving, if not greater, as much preventive effect as four injections initially, alleviating the suffering of the patient, and reducing the prevention cost.

The invention discloses recombinant vectors. The recombinant vectors are recombinant plasmids pcDNA3.1-SPME carried by recombinant bacteria DH5alpha / pcDNA3.1-SPM, the collection number of the recombinant plasmids in the China center for type culture collection (CCTCC) is CCTCC NO:M2015570. The invention further discloses a kit for preparing epidemic encephalitis B pseudoviruses and an application thereof. The invention further discloses a preparing method of the pidemic encephalitis B pseudoviruses and the recombinant bacteria. By means of the constructed recombination vectors pcDNA3.1-SPME, the epidemic encephalitis B pseudoviruses can be efficiently obtained, the titer of the obtained pidemic encephalitis B pseudoviruses can be high, and good application prospects can be achieved.

The invention provides an ACY W135 group meningococcal polysaccharide type-b haemophilus influenzae conjugate vaccine. A frozen-dry ACY W135 meningococcal polysaccharide vaccine is prepared by using the technology of removing endotoxin through ultracentrifugation so as to ensure that the endotoxin content of ACY W135 group meningococcal polysaccharide stock solution is greatly reduced before centrifugation. The conjugate vaccine can prevent disease infection of epidemic encephalitis for a patient by one dosage of injection, reaches and exceeds the preventing effect of the prior five dosages of injection, reduces the pain of the patient, and lowers the prevention cost.

The invention discloses an epidemic encephalitis polysaccharide-protein conjugated vaccine and a preparation method thereof. The vaccine is represented by [S-C]n-[N-P]m, wherein S represents an epidemic encephalitis polysaccharide molecule; C represents an atom C into which an aldehyde group is introduced in the epidemic encephalitis polysaccharide molecule; P represents a CRM197 protein molecule; N represents an atom N of lysine side chain amine in the protein molecule; and the epidemic encephalitis polysaccharide is at least one of a W-135 type epidemic encephalitis polysaccharide and a Y type epidemic encephalitis polysaccharide. In the vaccine disclosed by the invention, the Y type and W-135 type epidemic encephalitis polysaccharides are not subjected to a depolymerization technical process, and the Y type and W-135 type epidemic encephalitis polysaccharides are connected with proteins without using any connector. The method has the advantages of simple process flow, high yield, low production cost, relatively stable polysaccharide-protein ratio, capability of performing relative manual control in a horizontal range, and great improvement on the quality stability of the vaccine.

A Chinese traditional medicine for curing epidemic type-B encephalitis belongs to a Chinese traditional medicine for curing type-B encephalitis. The ingredients of the medicine are 40g of plaster stone, 10g of rehmannia root, 6g of rhinoceros horn, 5g of coptis, 5g of liquorice, 5g of bamboo leaves, 8g of pittosporm roots and 8g of balloon flower roots; the auxiliary ingredients are 15g of scorpion and 15g of hooked uncaria under the situation of twich and lockjaw; 10g of tabasheer and 10g of articifical bezoar under the situation of much sputum; 12gof moutan bark under the situation of dark purple complexion or maculae on the skin. The medicines used in the prescription are all natural herbs and the Chinese traditional medicine is processed with traditional method; the fetching of material is convenient and the prescription and manufacture method are simple; the expense for manufacturing the medicine is low. The composition of the Chinese herbal medicine is simple and the medicines used in the prescription are all natural ones and the fetching and use are convenient; the manufacture method is simple and the effect is excellent; the price is low; in this way, the herbal medicine is especially fit for people living in remote villages far away from counties and towns; the curing expense for patients with the disease of epidemic type-B encephalitis is low which solves the problem that the household income is low and the life is poor and the medical conditions is deficient locally.

The invention discloses a traditional Chinese medicine, in particular to a traditional Chinese medicine for treating epidemic encephalitis B. The medicine comprises the following raw materials in certain weight ratio: mix-fried licorice, chrysanthemum, oriental waterplantain rhizome, amur corktree bark, turmeric root-tuber, platycladi seed, juncus effuses, fructus forsythiae, bile, vietnamese sophora root, white stiff silkworm, cynanchum glaucescens, platycodon root, Chinese magnoliavine fruit, scorpion, bamboo juice, lalang grass rhizome and common bletilla pseudobulb. The medicine is simple in preparation, good in absorption effect, obvious in curative effect, free of side effects and high in safety, and is beneficial to timely alleviating symptoms of the disease.

The present invention discloses an isatis root health-care tea. It is mainly made up by using isatis root, artificial synthetic bovine bezoar and cane sugar according to a certain weight ratio. Said health-care tea has the functions of clearing away heat and removing toxic material, so that it can obtain good health-care effect for patients with the diseases of influenza, epidemic encephalitis, encephalitis B, pneumonia, hepatitis and viral common cold, etc.

The invention discloses a visual gene chip and kit used for detecting porcine epidemic encephalitis b viruses and / or hog cholera viruses. With the visual gene chip prepared by using a specific method, detection results can be visually seen with naked eyes, and an expensive laser confocal scanner is not needed; and the visual gene chip and kit have the advantages of low cost, high sensitivity which is almost equal to sensitivity of conventional fluorescence labeling methods, good specificity, short detection time, rapid detection and good application prospects.

The invention discloses an encephalitis-related virus detection kit and application thereof. The kit is used on the basis of the principles of multiplex PCR (polymerase chain reaction) combined nucleic acid intrusive reaction and nano gold color development. The kit comprises a combination of five types of primers and probes, and can be used for simultaneously detecting eastern equine encephalitis virus, western equine encephalitis virus, epidemic encephalitis b virus, West Nile virus and Nipah virus. When being used for detecting the five viruses, the kit has the advantages of high specificity and high sensitivity, and does not need any expensive apparatus; the detection result can be observed by naked eyes; and thus, the kit can be applied to the basic level.

The invention provides an application of calreticulin (CALR). The calreticulin (CALR) is used for restraining duplication of viruses especially Japanese encephalitis viruses (Japanese encephalitis virus, JEV) in host cells, so that diseases caused by virus duplication of JEV can be resisted, and disease resistance in accordance with epidemic encephalitis B is provided. The invention also providesa cell strain for resisting virus infection and a non-human mammal animal model for resisting epidemic encephalitis B, and a preparation method of the non-human mammal animal model for resisting epidemic encephalitis B. The CALR is highly conservative in mammals, so that the CALR can be widely applied to prevention and treatment of anthropozoonosis, besides, the CALR can be used as a potential target point for gene editing animal design, drug abuse is reduced, generation of epidemic situation is avoided, and the CALR has high economic value.

The invention discloses a visualized reverse transcription loop-mediated isothermal amplification kit for a swine epidemic encephalitis B virus and application of the kit. The kit comprises RT-LAMP primers, two reaction buffer solutions, an EM, a fluorescent visual detection reagent, ultrapure water and a swine epidemic encephalitis B virus RNA template; the RT-LAMP primers comprise the outer primers F3 and B3, the inner primers FIP and BIP and the loop primers LF and LB, and the application includes detection of a swine epidemic encephalitis B lesion tissue sample and toxic mosquitoes through the kit. Specificity detection and sensitivity detection prove that the reverse transcription loop-mediated isothermal amplification kit can monitor a reaction in real time and quantitatively detect out the copy number of the swine epidemic encephalitis B virus to quickly and accurately obtain a detection result, and convenience is brought to easy, convenient, quick and reliable detection of the swine epidemic encephalitis B virus.

A process for preparing Chinese medicine "Effervascent particles of southern isatis root" for treating cold, epidemic encephalitis B, hepatitis, parotitis, etc includes such steps as decocting 2 Chinese-medicinal materials, concentrating, spray drying, mixing with effervascent agent and additive, and granulating. Its advantages are high dissolving speed, agreeable taste and high curative effect and safety.

The present invention discloses an anti-flu health preserving traditional Chinese medicine tea drink and a preparation method thereof. The anti-flu health preserving traditional Chinese medicine tea drink is composed of the following main raw materials in parts by weight: 10 parts of each of isatis tinctoria, dyers woad leaves and corydalis bungeana, 5 parts of each of peucedanum praeruptorum and honeysuckles, and 10 parts of green tea. The traditional Chinese medicine tea drink preparation method comprises the following steps: step 1, weighing 10 parts of each of isatis tinctoria, dyers woad leaves and corydalis bungeana, and 5 parts of each of peucedanum praeruptorum and honeysuckles, conducting steaming and boiling sterilization for 20-30 minutes using a pressure cooker, and air-drying and grinding the sterilized materials into rough powder to obtain sterile raw materials; and step 2, adding 10 parts of green tea into the sterile raw materials to obtain the health preserving traditional Chinese medicine tea drink. The beneficial effects of the health preserving traditional Chinese medicine tea drink are that the health preserving traditional Chinese medicine tea drink has a slight scent of traditional Chinese medicines, has not any toxic or side effects, has obvious efficacies of clearing heat and toxic materials, and dispelling wind and heat, and is used for the treatment of epidemic influenza, epidemic encephalitis, epidemic hepatitis and upper respiratory tract infection symptoms caused by pathogenic wind- toxicity and hot disturbance.