The invention discloses a preparation method of group A/C meningococcal polysaccharide. The preparation method comprises the following steps: (1) carrying out fermentation culture on meningococcus, and carrying out sterilization treatment, thus obtaining a sterilized culture solution; (2) preparing meningococcus capsular polysaccharide; and (3) dissolving the rough polysaccharide, carrying out chromatography elution, collecting target peaks containing the polysaccharide, carrying out desalting on the collected polysaccharide solution by adopting an ultrafiltration membrane and adopting water for injection as an ultrafiltrate, thus obtaining the desalted solution, namely, the group A/C meningococcal polysaccharide solution. According to the preparation method, firstly, sterilization treatment is carried out, so that the inactivated culture solution is obtained, then, the capsular polysaccharide is prepared, and then, the capsular polysaccharide is purified by adopting the chromatography method; the use of abundant phenol in the traditional purification method is avoided, on the basis that the purification quality is guaranteed, the destruction of phenol to the environment is effectively avoided, meanwhile, the recovery rate of polysaccharide is approximate to that of the traditional phenol extraction method, in addition, the operation is simple, and the preparation method is suitable for large-scale production.