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Polysaccharide combined vaccine for epidemic encephalitis and preparation method thereof

A combined vaccine and polysaccharide technology, applied in medical preparations containing active ingredients, organic active ingredients, antibody medical ingredients, etc., can solve problems such as the absence of quadruple meningococcal polysaccharide vaccines, and reduce prevention costs and endotoxins. The effect of reducing content and reducing pain

Inactive Publication Date: 2009-05-13
长春长生生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is no public literature report on the quadruple meningococcal polysaccharide vaccine for groups A, C, W135 and Y suitable for the Chinese population

Method used

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  • Polysaccharide combined vaccine for epidemic encephalitis and preparation method thereof
  • Polysaccharide combined vaccine for epidemic encephalitis and preparation method thereof
  • Polysaccharide combined vaccine for epidemic encephalitis and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: 200502 batches of ACYW 135 Preparation of Meningococcal Polysaccharide Vaccine

[0044] 1. Take group A meningococcus 29201 strain, C group meningococcus 29205 strain, Y group meningococcus 29028 strain, W 135 Group meningococcus 29037 strain, inoculated on ordinary agar medium containing 10% sheep blood, meningococcus does not grow at 25 ℃. Cultivate in a carbon dioxide environment at 35-37°C for 16-20 hours, and grow smooth, moist, off-white colonies. The bacterial lawn is easy to remove, and it appears as a uniform suspension in physiological sodium chloride solution. Staining microscopic examination should show Gram-negative diplococci and monococci.

[0045] 2. Use culture tanks for liquid culture. During the cultivation process, samples were taken for pure bacteria inspection, and smears were used for Gram staining microscopy. If any contaminated bacteria were found, they should be discarded. The culture was terminated at the late logarithmic growth...

Embodiment 2

[0054] Example 2: 200503 batches of ACYW1 35 Preparation of Meningococcal Polysaccharide Vaccine

[0055] 1. Take group A meningococcus 29201 strain, C group meningococcus 29205 strain, Y group meningococcus 29028 strain, W 135 Group meningococcus 29037 strain, inoculated on ordinary agar medium containing 10% sheep blood, meningococcus does not grow at 25 ℃. Cultivate in a carbon dioxide environment at 35-37°C for 16-20 hours, and grow smooth, moist, off-white colonies. The bacterial lawn is easy to remove, and it appears as a uniform suspension in physiological sodium chloride solution. Staining microscopic examination should show Gram-negative diplococci and monococci.

[0056] 2. Use culture tanks for liquid culture. During the cultivation process, samples were taken for pure bacteria inspection, and smears were used for Gram staining microscopy. If any contaminated bacteria were found, they should be discarded. The culture was terminated at the late logarithmic growth...

Embodiment 3

[0065] Example 3: 200504 batches of ACYW 135 Preparation of Meningococcal Polysaccharide Vaccine

[0066] 1. Take group A meningococcus 29201 strain, C group meningococcus 29205 strain, Y group meningococcus 29028 strain, W 135 Group meningococcus 29037 strain, inoculated on ordinary agar medium containing 10% sheep blood, meningococcus does not grow at 25 ℃. Cultivate in a carbon dioxide environment at 35-37°C for 16-20 hours, and grow smooth, moist, off-white colonies. The bacterial lawn is easy to remove, and it appears as a uniform suspension in physiological sodium chloride solution. Staining microscopic examination should show Gram-negative diplococci and monococci.

[0067] 2. Use culture tanks for liquid culture. During the cultivation process, samples were taken for pure bacteria inspection, and smears were used for Gram staining microscopy. If any contaminated bacteria were found, they should be discarded. The culture was terminated at the late logarithmic growth...

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Abstract

The invention provides an epidemic meningitis polysaccharide combined vaccine, and aims to prepare a freeze-dried ACYW135 meningococcal polysaccharide vaccine by the process of removing endotoxins by the ultracentrifugation method so as to ensure that the content of endotoxins present in ACYW135 group meningococcal polysaccharide stock solution is far lower than that before the centrifugation. The vaccine provided by the invention has the advantages of ensuring that only one injection is enough to prevent a patient from being infected with epidemic meningitis disease, achieving, if not greater, as much preventive effect as four injections initially, alleviating the suffering of the patient, and reducing the prevention cost.

Description

Technical field: [0001] The invention relates to a preparation method of a meningococcal polysaccharide combined vaccine, in particular provides a meningococcal polysaccharide combined vaccine preparation and a preparation process for the immune prevention of important cerebrospinal meningitis diseases, and belongs to the technical field of vaccine production and preparation. Background technique: [0002] Epidemic meningitis is bacterial meningitis (referred to as meningitis) caused by meningococcus (Meningococcus), which is an acute respiratory infectious disease. The main clinical manifestations are fever, headache, vomiting, skin and mucous membrane petechiae, ecchymosis and meningeal irritation. Severe cases may have septic shock and meningoencephalitis. The disease has a sudden onset and a high mortality rate, and can occur in all age groups, and children under the age of 15 account for more than 75% of the cases. In order to effectively control the occurrence of men...

Claims

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Application Information

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IPC IPC(8): A61K39/095A61K31/715A61K9/19A61P31/04
Inventor 杨宗辉孙惠军巨爽李冲
Owner 长春长生生物科技股份有限公司
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