172 results about "Gram staining" patented technology

Bacilliis natto NLSSc with the preservation number CGMCC No. 0724 is Gram staining positive, non-acid fast, straight or near straight, (0.6-0.8)x(1.5-2.0) micron sized and aerobic; has mesial and un-swelled spore with the spore number inside one sporange cell not more than one, meso diamino heptane diacid and glycin contained in the cell wall, no characteristic saccharum and peripheral flagellum; and can be grown well in five kinds of natural organic culture medium and can form relatively thick mycoderm and butter fat colored colony with smooth edge in soybean cake powder culture medium. Bacilliis natto can be cultured in culture medium with nitrogen source to obtain thrombus dissolving matter natto kinase and the cultured matter may be extracted to obtain powdered matter as the additive for thrombolytic health food.

The present invention provides Bacillus vallismortis SZ-4, which has the preservation number of CGMCC No.8273. According to the present invention, the strain grows on a beef extract-peptone (NB) culture medium in a single cell reproduction growth manner to form bacterial colonies, and the bacterial colonies have characteristics of round shape, milk white color, slightly elevated center, and moist and transparent surface; microscopic examination results show that the Bacillus vallismortis SZ-4 has a rod shape, has flagella, has a size of 0.7-0.82*1.6-2.2 mum, presents the positive Gram staining effect, has spores, and can grow on a beef extract-peptone (NB) culture medium containing 2-5% of NaCl, wherein the beef extract-peptone (NB) culture medium comprises: 3.0 g of a beef extract, 10.0 g of peptone, 5.0 g of NaCl, 17 g of agar and 1000 ml of water, and the pH value is 6.8-7.2; and the Bacillus vallismortis SZ-4 provides significant antagonism effects for pathogenic bacteria causing ginseng root rot, blight, sclerotinia sclerotiorum and botrytis cinerea. The present invention further provides an application of the Bacillus vallismortis SZ-4 in prevention and control of plant fungal diseases or preparation of microorganism preparations for prevention and control of plant fungal diseases.

The invention relates to a multifunctional fully-automatic Gram staining instrument. The instrument comprises a glass slide lateral movement device, a microbe automatic staining device, a glass slide entering and departing device, a push rod device and a liquid path control device, the push rod device pushes a coated glass slide positioned on the glass slide entering and departing device into the glass slide lateral movement device, the glass slide moves with a first glass slide rack on a reciprocating movement mechanism to the automatic staining device, is stained, cleaned and blow-dried, the liquid path control device cooperates a computer program in order to control a staining solution in a Gram staining solution bottle to carry out staining, water spraying, gas spraying, drying and other fully-automatic activities on the coated glass slide according to preset steps and time, and the push rod device moves the processed glass slide into the glass slide entering and departing device in order to carry out subsequent operation. The instrument has the advantages of fully-automatic realization of the whole Gram staining process, accurate delivery, cross infection avoiding, staining solution waste avoiding and manpower resource saving.

The invention discloses pediococcus pentosaceus and application thereof. The Latin name of the pediococcus pentosaceus is Pediococcus pentosaceus LI05, and the pediococcus pentosaceus is preserved in the China General Microbiological Culture Collection Center and has a preservation number of CGMCC No.7049. The pediococcus pentosaceus disclosed by the invention has the morphological characteristics that the thallus is rod-shaped, dose not generate spores, does not have motility, and has masculine Gram staining. The whole-cell fatty acid of the pediococcus pentosaceus disclosed by the invention comprises the following main components in percentage by weight: about 1.45% of 14:0, about 3.26% of 16:1w7c/16:1w6c, about 31.25% of 16:0, about 6.31% of 18:1w9c, about 38.59% of 18:1w7c, about 1.37% of 18:0 and about 14.59% of un18.846/19:1w6c. The invention also discloses a complete sequence of 16S ribosome DNA (16SrDNA) of the pediococcus pentosaceus. The pediococcus pentosaceus and a preparation thereof disclosed by the invention can be used for adjusting the micro-ecological balance of intestinal tracts of human or animals, promoting digestive absorption and slowing down the happening and development of liver failure of experimental animals.

The invention belongs to the field of microorganism, particularly relates to ((i) bacillus cereus (/i)) Y-15 for efficiently degrading feather keratin, and further relates to application of the ((i) bacillus cereus (/i)) Y-15. The ((i) bacillus cereus (/i)) Y-15 is preserved in China General Microbiological Culture Collection Center in June 6, 2017, with the culture preservation number being CGMCCNO. 14221; the colonial morphology on a milk screening plate is as follows: bacterial colony is white and circular, the waxiness on the surface is non-transparent, and the edge is wavelike; gram staining shows a positive result, and the thallus is bacilliform. The ((i) bacillus cereus (/i)) Y-15 screened and cultured has excellent degradation effect on keratin.

The invention discloses rhodococcus ruber YMHL-1 capable of degrading nicosulfuron and applications thereof. The invention provides a strain namely rhodococcus ruber YMHL-1 capable of degrading residual nicosulfuron in wastewater, and the identification shows that the strain belongs to Rhodococcus. The strain is preserved in China General Microbiological Culture Collection Center, CGMCC in February 9th, 2015; and the preservation number is CGMCC No. 10542. The main biological characteristics are as follows: the Gram staining reaction is negative; the bacterial colony is in an orange yellow color, is in a round shape, and is opaque, the surface of the bacterial colony is rough and dry; the cell is in a sphere shape or short bar shape, does not have any flagellum, is motionless, and does not generate any spore; the strain is aerobic and chemoheterotrophic and cannot liquefy gelatin and hydrolyze starch; the enzyme contact reaction is positive, the V.P. reaction is positive, the methyl red reaction is negative; the strain cannot degrade casein, cannot reduce nitrates; and the strain can produce acids from glucose, can utilize glucose, fructose, and citrates, cannot utilize mannose, arabinose, and raffinose, and can reduce more than 90% of antibiotics in water through direct application.

The invention relates to an automatic staining instrument with a Gram staining two-step method. The staining instrument comprises two staining pools, a cleaning pool, a hot air flow drying pool, a manipulator, a rail and a display screen, wherein the first staining pool, the second staining pool, the cleaning pool and the hot air flow drying pool are sequentially arranged at the front part of the staining instrument, the manipulator is arranged at the upper part of the staining instrument, slices to be stained or quality control slices can be placed in the front end of the manipulator, the back end of the manipulator is connected with the rail, and the display screen is arranged at one side of the top of the staining instrument. Compared with the prior art, the instrument has the advantages that the protective technology and the operation flow are simple, special requirements to water quality are free, no harmful organisms are introduced and produced during the production and detection processes, more quick and easily-standardized experiment results can be provided, the result is easy to observe and judge, and the safety and reliability are provided.

A Gordona nitida LSSEJ-1 (CGMCC No.0700) and its application in removing sulfur from the S-contained organic compound are disclosed. Said rod-shaped Gordona nitida LSSEJ-1 has 2-3 microns in length, shows positive to Gram staining, and contains meso diaminopimelic acid, characteristic arabinose and galactose, nocardomycolic acid and MK-9 (H2).

A method for preparing degrading bacteria for eliminating residual triazine herbicide is disclosed. The strain is Gram's chromatic reacting positive strain BTAH1, which belongs to exiguobacterium sp. Its characteristics are G+, irregular nemaline, 0.7-1.2X2.0-4.0mum, contact enzyme is positive, and it can hydrolyze starch. Genbank landing number of the strain 16S rDNA is AY205564. Its advantages include decrement of pesticide residual amount, atoxic and innoxious green agricultural product.

The invention provides a prodegradant which can dispel the organic phosphorus pesticide residue of chlorpyrifos. The used strain is Gram's stain reaction hysteroptosis Dsp-2 which is Sphingomonas sp. The main biology characteristic is G-; the thaliana is the rod type of asporous whose size is 1.0-1.5um; the scavenger enzyme is male and the oxidative enzyme is female which can not be hydrolysed starched and be gelatin liquidated; the methyl red reaction is female and not to produce the indole with the aquolysis temperature 80 deg. The Genbank landing number of the strain 16S rDNA is AY994060.

The invention belongs to the field of examination of microorganisms, and relates to a method for quickly identifying bloodstream infection bacteria by a high-resolution fusion curve method. The method comprises the following steps of: performing smear gram staining on a blood culture positive culture flask to distinguish negative bacteria and positive bacteria; selectively amplifying different 16S fragments for the negative bacteria and the positive bacteria; and analyzing by the high-resolution fusion curve method. Common bloodstream infection pathogenic bacteria comprise staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, enterococcus faecium, Escherichia coli, Klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, enterobacter cloacae, Serratia marcescens, Klebsiella oxytoca, acinetobacter lwoffii, enterobacter aerogenes, stenotrophomonas maltophilia and burkholderia cepacia. By the method, the common bloodstream infection pathogenic bacteria can be measured quickly, efficiently and accurately, so that strong evidences are provided and precious time is striven for subsequent treatment.

The invention provides a degrading bacterium eliminating the pollution of triethylamine and a degrading microbial agent and belongs to the high-technical field of biology. The used strain is a gram staining reaction positive bacterium R4, is determined as arthrobacter protophormiae, and has a biological characteristic of G+. The logarithmic phase thallus is bacilliform, sporeless and aerobic to grow, and can grow by taking the triethylamine as the only carbon source or nitrogen source and thoroughly mineralize the triethylamine to generate ammonia nitrogen. In a flask experiment in a laboratory, the strain can completely degrade 100mg/L triethylamine and solve the problem of the difficult biodegradation of the triethylamine in wastewater treatment.

The invention provides a degradation strain for eliminating residual bromoxynil, belonging to the field of biological high technology. The adopted bacterial strain is a gram staining reaction negative strain MXX-04 which is identified to be paracoccussp. The main biological properties are G-, short rod shape, no spores, no motility and positive oxidase and catalase. The strain provided by the invention can grow by taking bromoxynil as a unique nitrogen source and mineralize the bromoxynil. Under the condition of a shake flask in a laboratory, the strain can degrade 95.3% of 100mg/L bromoxynil within 24 hours, thus the problem that bromoxynil is hard to degrade biologically in the environment is solved.

The invention relates to an Enterococcus faecium strain producing bacteriocin. The strain was preserved as a name Enterococcus faecium R1 by China General Microbiological Culture Collection Center on January 20, 2016, with preservation number CGMCC No.12085. A source of the strain is farm traditional fermented soybean paste and is presented as a circular or elliptic white smooth nontransparent colony smaller than 1mm in an MRS agar culture medium; uniform turbid growth is presented in an MRS liquid culture medium; thalli are spherical under an electron microscope and are arranged in pairs or in a chain, and gram staining is positive. The strain provided by the invention is sensitive to antibiotics, does not contain virulence genes and is a safe strain. The bacteriocin produced by the strain is enterococcin P, is stable under acidic conditions, has a very good thermal stability and has a good bacteriostatic effect on common enteropathogenic bacteria.

The invention discloses an identification method of bacillus subtilis with a high protease output. The method includes: inoculating a bacterial strain to be identified onto a skim milk powder plate culture medium, culturing at 35 DEG C for 24 h, performing Gram staining, performing microscopic examination, separately extracting a DNA template from 1.0 mL of the bacterial strain to be identified by adoption of a genome DNA extraction solvent kit, designing and synthesizing PCR amplification primers, with the upstream primer P1 being 5'-AGAGTTTGATCCTGGCTCAG-3' and the downstream primer P2 being 5'-AGTAAGGAGGTGATCCAACCGCA-3', and performing PCR amplification of 16SrDNA. The method is high in bacterial strain selectivity, scientific in identification method, short in time and period and high in efficiency.

The invention discloses a petroleum aromatic hydrocarbon degrading bacterium (G-40), which is named as brevibacillus laterosporus G-40 and belongs to brevibacillus. The strain has been saved in ChinaCenter for Type Culture Collection (CCTCC), Wuhan university, China in June 30, 2017. The preservation number is CCTCC No. M2017400. The strain is screened from petroleum polluted soil in the oil producing region of Guanghua oil field in Qianjiang city. The strain (G-40) has an ellipsoidal shape. The result of gram staining is positive. The strain (G-40) can degrade 53.02% of petroleum aromatic hydrocarbons after the strain is cultured for 30 days under following conditions: temperature: 35 DEG C; pH value: 7.3; oil concentration: 0.6%; salt concentration: 0.5%; inoculation amount: 10%; nitrogen source: (NH4)2SO4; and phosphor source: Na2HPO4. The strain (G-40) can adapt with wide ranges of temperature, pH value, and salinity, and has a good prospect and economic value in fields such as petroleum pollutant elimination, biological restoration of petroleum aromatic hydrocarbon polluted soil and water, and the like.

The invention provides degrading bacteria for eliminating residue of pesticide, namely bromoxynil octanoate, and a degrading bacterial agent thereof, and belongs to the technical field of biology high-technology. The strain is Gram staining reaction negative bacteria XB2 and is identified as Acinetobacter baumannii. The strain has main biological characteristics that: the strain is G-; the thallus has a short-rod shape with the size of 0.9-1.6*1.5-2.5 mu m, has no spores and is strictly aerobic; and the strain is negative for oxidase and positive for catalase and can grow by taking the bromoxynil octanoate as a unique carbon source. The degrading bacteria is directly applied to make bromoxynil octanoate residue in crops reduced by over 90 percent, solves the problem that the bromoxynil octanoate residue in agricultural production is overproof, and can produce non-toxic and nuisanceless green agricultural products.

The present invention provides a kind of bacterium and its procreation of living bacterium reagent, which can reduce the rate of piglet diarrhea. The used of bacterium is gelanshi pigmentation feedback electropositive bacterium S1, according to appraisal, it is Sporolactobacillus sp..Its main biological character is G+, the shape of bacterium is slim, divided burl, elliptical sporangium, after malachite pigmentation pigmentation is obvious, concurrently, exclude oxygen development, endure lower pH(pH2.5) and higher saline chroma(0.2%), not sensetive to pepsin, endure high temperature, it can restrain coliform and golden ball bacterium well. It indicated from the track of molecule biological technology, this bacterium can propagated in alvine of piglet. The flow chart is bevel bacterium--- rocky bottle seed liquid--- ferment jar---freez dryness--- production. The animal test indicates, this bacterium can be used directly and widely in reduce alvine of piglet, enhance the growth rate of piglet, reduce the use of antibiotics in piglet feedstuff.

The invention discloses a paracoccus versutus (Paracoccus versutus) strain 2#CGMCC No. 13321 and application thereof for oxidizing nitrate serving as an electron acceptor to remove hydrogen sulfide (H2S) in biogas under anoxic conditions. The strain is subjected to Gram staining to be negative, and the strain cells are in a shape of short rods and are small; the bacterial colony is white and bulged, the edge is neat and smooth, and the surface is wet; the strain is not salt-tolerant, and citrate cannot be utilized; and in the presence of nitrate, nitrite or nitrogen oxide, anaerobic growth of the strain can be promoted by taking nitrate, nitrite and nitrogen oxide as electron acceptors. After the paracoccus versutus strain CGMCC No.13321 is inoculated to a desulfurization reactor, the H2S can be subjected to biological oxidation in the reactor by taking the nitrate as the electron receptor and then is subjected to growth and propagation, the aim of removing the H2S in the biogas is achieved, the potential safety hazard brought by mixing of the introduced methane and oxygen in the traditional method for removing the H2S in the biogas by taking the oxygen as the electron acceptor is effectively avoided, and the removal rate of the H2S in the biogas is up to 93.8%.

The invention relates to a rapid detection method of coliform groups. In the method, the situation that coliform group cells can generate gas and ferment lactose to generate acid in a short-time culture process is utilized to combine the coliform group cells with eosin methylene blue to ensure that a mixed eosin methylene blue culture solution generates atropurpureus or purplish red precipitation, and the image number of negative bacteria is obtained after carrying out gram stain on the atropurpureus or purplish red precipitation through a biological microscope lens so as to count the number of the coliform groups, or the mixed eosin methylene blue culture solution changes colors because of the atropurpureus or purplish red precipitation generated by the mixed eosin methylene blue culture solution, and the cell number of the coliform groups is recognized by utilizing the characteristics that the color change degree of the mixed eosin methylene blue culture solution is related to the cell number of the coliform groups. The method is suitable to rapid detection of the coliform groups in the fields of biology, medicines, foods and various articles.