49 results about "Gram staining" patented technology

The invention relates to a bacterial pneumonia pathogenic bacterium identification method based on an intelligent Raman technology, which comprises the following steps: S1, setting a type label according to a gram staining characteristic classification mode or the occurrence frequency of bacterial types in bacterial pneumonia infection, and selecting a known strain according to the type label, collecting Raman spectrum data of a known strain; s2, carrying out wave number extraction, background subtraction, smooth noise reduction and normalization preprocessing on the Raman spectrum data; s3, constructing a classification model according to an AlexNet model; s4, dividing the preprocessed Raman spectrum data into a training set and a verification set, and inputting the training set, the verification set and corresponding type labels into a classification model for training to obtain a trained classification model; and S5, acquiring Raman spectrum data of detected bacteria by using a Raman spectrometer, preprocessing the Raman spectrum data, and inputting the preprocessed Raman spectrum data into the trained classification model to obtain the type of the detected bacteria. The bacteria are identified by establishing the classification model, so that the operation complexity is reduced, and the identification time is shortened.

The invention relates to a strain of Chryseobacterium sp. ZJUHULYY1 and application of the Chryseobacterium sp. ZJUHULYY1 in promotion of corn growth and iron absorption, and provides a strain of Chryseobacterium sp. ZJUHULYY1, which is preserved in China Center for Type Culture Collection on April 27, 2025, and has a preservation number of CCTCC NO: M 2025904. The strain of Chryseobacterium sp. ZJUHULYY1 is obtained by separating and purifying from rhizosphere soil of corn, and the strain of Chryseobacterium sp. ZJUHULYY1 is obtained by separating and purifying from rhizosphere soil of corn. The biological characteristics of the strain are as follows: after being cultured in an LB plate culture medium at 30 DEG C for 24 hours, the bacterial colony is round or oval, golden yellow, smooth and glossy in surface, neat in edge and negative in gram staining; when the strain is inoculated to rhizosphere of corn growing in calcareous soil, compared with a control group, the plant height, chlorophyll content, biomass and active iron content of the corn are all remarkably improved, the growth condition of the corn in the calcareous soil is effectively improved, and the absorption and utilization efficiency of the corn on iron elements is improved.

The invention belongs to the technical field of microbiology, and particularly relates to application of a high-cadmium-resistance strain Cd2 in promoting repair of heavy metal pollution of aster katsumadai. The invention relates to a strain Cd2 with high cadmium resistance, the taxonomic name of the strain Cd2 is Pseudomonas aeruginosa, and the preservation number of the strain Cd2 is GDMCC No.64110. The strain Cd2 has the advantages that the strain Cd2 has high cadmium resistance; according to the invention, screened strains are identified through morphological observation, gram staining and 16S rRNA (ribosomal Ribonucleic Acid) sequence sequencing, and a high-cadmium-tolerance strain Cd2 is inoculated into soil during the growth period of a hyperaccumulator Aster brachium, so that the influence of Cd2 on the growth and enrichment of the hyperaccumulator Aster brachium is researched. The result shows that when the high-cadmium-tolerance strain Cd2 is inoculated into the soil for planting the aster katsumadai, the cadmium absorption amount of the underground part and the overground part of the aster katsumadai and the cadmium accumulation amount of the whole plant are remarkably increased, and a technical support can be provided for strengthening hyperaccumulation plant remediation.

The invention belongs to the field of biological quarantine, and particularly relates to a method for separating and identifying pigeon-derived liver escherichia coli. Escherichia coli separation and identification are carried out on disease samples from Guangdong, Guangxi, Guizhou, Hainan and the like, and the epidemicity of the disease samples is determined; collecting livers of acute dead young meat pigeons, and separating strains; carrying out gram staining, physiological and biochemical identification and molecular identification on the separated strain; the growth and reproduction cycle and hemolysis characteristics of the strain are detected, 76 Escherichia coli infection occurs in 113 disease samples, and the infection rate is 67%. According to the method, laboratory diagnosis of infectious diseases of the meat pigeons is systematically completed through sample collection and separation, culture and identification of strains, it is determined that the young meat pigeons are infected by escherichia coli, and a foundation is laid for subsequent antibiotic-free green breeding of the meat pigeons resisting escherichia coli infection.

The present invention provides a sputum sample bacteria detection method based on YOLOV8, which belongs to the field of image processing and computer vision technology; specifically comprises the following steps: step 1: obtaining a Gram-stained slide image data set, and preprocessing the obtained Gram-stained slide image data set; step 2, data labeling: performing rotating frame data labeling on the preprocessed data in step 1, and dividing the data into a training set, a validation set, and a test set; step 3, improving the network model: modifying the loss function, improving YOLOV8 in the feature extraction and feature fusion stages, and obtaining an improved model; step 4, training the model: inputting the training set into the network model for iterative training, and obtaining an optimal target detection model; step 5, evaluating the model. The present invention realizes accurate and rapid bacterial instance segmentation and recognition, and can significantly improve the detection accuracy and efficiency of bacteria, and improve accuracy and credibility.

The invention relates to the field of medical detection, in particular to a fluorescent sensing composition, a fluorescent sensing film and application thereof. According to the invention, by screening a plurality of fluorescent indicators and combinations thereof, an indicator formula with simple components and low cost is finally obtained. Based on the formula, by optimizing the preparation process, the high-efficiency fluorescent sensing film resistant to damp-heat sterilization is developed. After a microorganism culture bottle prepared on the basis of the membrane is inoculated with Gram-negative bacilli, positive cocci and yeast fungi representative quality control strains, experiments of full-automatic microorganism culture system fluorescence curve determination, Gram staining, microorganism mass spectrum identification and the like prove that the fluorescence sensing membrane is excellent in detection performance. The fluorescent sensing film provided by the research is applied to clinical microorganism detection, can provide technical support for blood flow infection diagnosis, and is of great significance in suppressing antibiotic abuse and improving prognosis of blood flow infection patients.

The application discloses a triazine herbicide propoxur degrading bacterial strain and application thereof, the bacterial strain is SWFU-DH02 strain which is negative in gram staining reaction and is identified as Microbacterium sp. Exiguobacteruim sp. ) on November 22, 2021, and is deposited in the China General Microbiological Culture Collection Center, and the strain preservation number is CGMCC No. 23948. The application discloses, for the first time, the degradation effect of Microbacterium sp. Exiguobacteruim sp. ) on triazine herbicide propoxur, and a Microbacterium sp. high-efficiency degrading bacterium SWFU-DH02 is screened, the degradation efficiency of the bacterium on propoxur can reach 75.08%, and the bacterium grows optimally under the conditions of pH 7.0, temperature 28 DEG C and salt concentration 1%. The degrading bacterial strain SWFU-DH02 has the characteristics of simple culture, rapid growth and high efficiency of degradation. The application can solve the pollution problem of triazine herbicide propoxur in soil or water, prevent the phytotoxicity of herbicide residues on subsequent crops, and produce non-toxic and non-polluting green agricultural products. The application provides a new biological approach for modern pesticide pollution control.

The invention discloses a teaching evaluation method for a gram staining experiment of bacteria, which is used for solving the problems of strong subjectivity, low efficiency and difficulty in comprehensively covering experimental operation details due to dependence on manual observation and recording in a traditional mode. Acquiring an operation image when an experimenter performs normal saline dropwise adding operation, and calculating a normal saline dropwise adding score according to the operation image; collecting the temperature of the inoculating loop to calculate the use score of the inoculating loop, detecting and identifying the bacterium taking operation of the inoculating loop, and calculating the bacterium taking score of the inoculating loop; continuous frame extraction identification is carried out on the linear distance change of a glass slide and an alcohol lamp, and smear and drying scores are calculated; carrying out normative judgment on the gram staining operation in sequence, calculating a gram staining score, and carrying out imaging color identification on a staining result to obtain a microscopic examination score; and obtaining a comprehensive teaching score by integrating the normal saline dropwise adding score, the inoculating loop use score, the inoculating loop bacterium taking score, the smear and drying score, the Gram staining score and the microscopic examination score.

The invention provides a bacillus high-yield strain for producing riboflavin and application of the bacillus high-yield strain, and belongs to the technical field of microbial fermentation. The bacillus strain is named as VB2-M-230602-66, the preservation unit is China Center for Type Culture Collection, the preservation date is December 1, 2023, and the preservation number is CCTCC NO: M20232421; according to the invention, the strain VB2-M-230602-66 with stable character is obtained by adopting physical mutagenesis separation and combining with shake flask screening of tolerance dose of a resistance marker, riboflavin can be produced through liquid fermentation, and the yield is stable; the bacterial strain has the biological characteristics that the bacterial strain is gram-positive bacteria through gram staining, the bacteria are cultured at 37 DEG C for 18 hours, the thalli are rod-shaped, 0.5-0.8 mu m * 2.0-5.4 mu m and are arranged in a single or paired manner, and bacterial colonies cultured on a solid culture medium are pale yellow, nearly circular, wet in surface, opaque and irregular in edge; the bacillus strain VB2-M-230602-66 provided by the invention is suitable for fermentation production of riboflavin (vitamin B2), has stable production performance, can obtain greater economic benefits, and is worthy of popularization.

The invention discloses a landfill leachate degrading bacterium S14 and belongs to the technical field of microorganisms, the landfill leachate degrading bacterium is bacillus subtilis and is preserved in the China Center for Type Culture Collection, the address of the preservation unit is Wuhan University, Wuchang District, Wuhan City, Hubei Province, and the preservation number is CCTCC M 2025283. Preferably, the bacterial colony is grey white, approximately circular and opaque, and wrinkles are formed in the middle of the bacterial colony. Preferably, gram staining is positive, and thalli are slender and rod-shaped. According to the invention, the S14 strain can effectively degrade main pollutants in the landfill leachate, including COD (Chemical Oxygen Demand), ammonia nitrogen and total phosphorus. In an experiment, the removal rates of the S14 strain on ammonia nitrogen, COD (Chemical Oxygen Demand) and total phosphorus respectively reach 88.84%, 55.88% and 82.68%.

The utility model provides a sterilization glass slide convenient for gram staining and identification, which comprises a glass slide main body, a sample information marking area, a standard bacterium area and a sample bacterium area are sequentially arranged on the front surface of the glass slide main body, and the sterilization glass slide is characterized in that the sample information marking area, the standard bacterium area and the sample bacterium area are sequentially arranged along the length direction of the glass slide main body; the front surface of the glass slide main body is partitioned through partition straight lines coated with the front surface of the glass slide main body, the partition straight lines are arranged in the width direction of the glass slide main body, and the multiple partition straight lines are sequentially distributed at intervals in the length direction of the glass slide main body; separation round lines are respectively arranged in the standard bacterium area and the sample bacterium area; and the thicknesses d of the separation straight lines and the separation round lines are 0.5-2 mm, and the widths D of the separation straight lines and the separation round lines are 0.5-2 mm. According to the utility model, the cross contamination problem in the staining process can be effectively improved, false positive and false negative phenomena are avoided, the accuracy of a staining result is improved, and the operation convenience, the result accuracy and the test efficiency of gram staining and identification of sample bacteria are improved.

The invention discloses a strain of Bacillus CAU-D1, a biological microbial agent and an application thereof, comprising Bacillus CAU-D1, wherein the colony color of the Bacillus CAU-D1 on an LB culture medium is white with yellowish color, is opaque, round or nearly round, has an unsmooth edge and has obvious wrinkles on the surface; the physiological and biochemical characteristics of the Bacillus CAU-D1 are Gram-positive, have the functions of nitrogen fixation, inorganic phosphorus dissolution and potassium dissolution, and have no chemotaxis; the bacterial content of the Bacillus CAU-D1 in the biological microbial agent is 1×10 8 The Bacillus CAU-D1 provided by the present invention has the effects of nitrogen fixation, phosphorus solubilization and potassium solubilization on the plate, and has a significant promoting effect on soybean growth and root structure improvement under pot test conditions, thereby providing high-quality resources for Bacillus resource research and the development and application of adaptive biocontrol strains.

The invention relates to the technical field of mycoplasma staining, in particular to a simple and efficient mycoplasma staining detection method. The mycoplasma detection method is different from a DNA fluorescent staining detection kit method and a PCR method, the adopted staining solution is a common Gram staining solution, is non-toxic and low in price, and overcomes the limitation and defects of the traditional mycoplasma detection method, such as toxic detection reagents, harsh detection conditions, expensive detection instruments, complex and tedious detection methods and the like. The method has the advantages of simplicity in operation, low cost, good repeatability, good dyeing effect and the like, provides a novel detection method for mycoplasma detection, and is extremely wide in application.

The invention discloses bacillus velezensis Jcl-10 and application of the bacillus velezensis Jcl-10 in prevention and treatment of vibrio cholerae. The strain Jcl-10 is preserved in the China Center for Type Culture Collection, the preservation time is January 25, 2024, and the preservation number is CCTCC N: M 2024212. The bacillus velezensis Jcl-10 disclosed by the invention is identified as a gram positive bacterium after being subjected to gram staining. The bacterial strain can produce antibacterial peptide substances, antibacterial protein substances, polyketide synthetase substances and the like with bacteriostatic action, and can be well applied to prevention and treatment of aquatic diseases caused by vibrio cholerae. The Jcl-10 is added into the aquatic feed, so that the morbidity of aquaculture animals can be effectively reduced, and the economic yield of the aquaculture animals can be increased. In conclusion, the bacillus velezensis Jcl-10 disclosed by the invention has outstanding performance in the prevention and treatment of vibrio cholerae, and provides a powerful guarantee for continuous and healthy culture of aquatic products.

The invention relates to a novel bacterial strain capable of degrading pesticides, in particular to a novel bacterial strain Acinetobacter oryzae F9-1, and a preparation method of the novel bacterial strain Acinetobacter oryzae F9-1. A new strain Acinetobacter oryzae F9-1 is obtained by separation, screening and identification from a soil sample in Hami region of Xinjiang, the strain is subjected to physiological and biochemical characteristic detection, the new strain F9-1 shows that the bacterial colony is small, the surface is smooth, the new strain F9-1 is white and semitransparent on an NB plate, the results of Gram staining, catalase and indole experiments and the like are positive, and the results of oxidase, V.P tests, urease and the like are negative. The strain F9-1 can hydrolyze hydrogen peroxide; l-alanine, lactic acid, L-aspartic acid, quinic acid and the like can be utilized, the strain is sensitive to 8% NaCl, lincomycin, nalidixic acid, chloramphenicol and the like, fluridone and pendimethalin can be degraded, and the strain has the advantages of being environmentally friendly, efficient, economical, sustainable and the like and is an effective means for solving the pesticide pollution problem.

The present invention generally relates to improved methods for characterizing the cell wall complex composition of a bacterial specimen via differential transport kinetics of a small molecule. The present invention also relates to improved and automated methods for Gram differentiation of a bacterial specimen using nonlinear optical techniques.

The invention discloses a bacillus CAU-D1, a biological agent and application. The bacillus CAU-D1 comprises the bacillus CAU-D1, and a bacterial colony of the bacillus CAU-D1 on an LB culture medium is yellow in white, opaque, round or nearly round, unsmooth in edge and obvious in surface wrinkles; the physiological and biochemical characteristics of the bacillus CAU-D1 are gram staining positive, and the bacillus CAU-D1 has the functions of fixing nitrogen and dissolving inorganic phosphorus and potassium and has no chemotaxis; the bacterium content of the bacillus CAU-D1 in the biological agent is 1 * 10 < 8 > cfu / ml. The bacillus CAU-D1 provided by the invention has the effects of fixing nitrogen, dissolving phosphorus and dissolving potassium on a flat plate, and has an obvious promoting effect on soybean growth and root configuration improvement under a pot experiment condition, so that a high-quality resource is provided for bacillus resource research and development and application of adaptive biocontrol strains.

The invention discloses pediococcus acidilactici capable of degrading nitrite and application of the pediococcus acidilactici capable of degrading nitrite. The pediococcus acidilactici capable of degrading nitrite is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.33428. Gram staining of the pediococcus acidilactici is positive, catalase contact test is negative, a 16S rDNA sequence is shown as SEQ ID NO: 1, the nitrite degradation rate of the pediococcus acidilactici is larger than 95%, and the pediococcus acidilactici grows well under the salt concentration of 4% or above. The invention further discloses an application of the nitrite degrading pediococcus acidilactici Pac.G1 in preparation of low-nitrite brassica campestris. Compared with the prior art, the brassica campestris prepared by applying the pediococcus acidilactici capable of degrading nitrite, disclosed by the invention, has the advantages that the nitrite content is obviously reduced, the chewiness, the vitamin C content, the total acid content and the delicious amino acid content of the product are higher, the quality, the taste and the flavor are better, and safer and healthier pickled vegetable products are provided for consumers.

The invention belongs to the technical field of environmental microorganism screening, and discloses a methanol bacterium and application thereof. The method comprises the following steps: adding high-arsenic sediment into an aerobic inorganic salt culture medium taking methanol as a unique carbon source, and placing in a shaking table at 30 DEG C for dark culture at a constant rotating speed; after enrichment and passage, diluting a bacterial solution obtained by culture, uniformly coating the diluted bacterial solution on a solid culture medium taking methanol as a unique carbon source, and placing the solid culture medium in a constant-temperature incubator at 30 DEG C for culturing in a dark place; selecting single colonies from the grown bacterial colonies, selecting the selected single colonies to a new solid culture medium for lineation, placing the new solid culture medium in a constant-temperature incubator for culture, and repeating lineation for more than three times after the single colonies grow to obtain the pure bacterial beads utilizing methanol. The strain can be stored in a liquid inorganic salt culture medium containing 300 mg / L of As < 3 + > and has good arsenic resistance. Gram staining experiment is negative, 16s. RRNA sequence identification shows that the arsenic-resistant strain is methylotrophicus methylobacterium, and strain resources are provided for microbial remediation of arsenic-polluted environment.

The application discloses a gynecological micro-ecological detection system based on AI multi-modal fusion, and belongs to the technical field of gynecological micro-ecological detection. The system comprises a data acquisition module, a standardization module and a micro-ecological output unit. The data acquisition module acquires immunofluorescence signals, gram staining morphological characteristics and dry chemical indexes. The data acquisition module is connected to the standardization module. The standardization module comprises a pre-processing unit for pre-processing collected data. The standardization module is connected with a data classification unit for data classification. The data classification unit is connected to a heterologous data normalization processing unit. The gynecological micro-ecological detection system based on AI multi-modal fusion can realize accurate evaluation of gynecological micro-ecology, construct a dynamic model of microbial community parameters, realize inflammation process quantification, generate a visual micro-ecological atlas through three-dimensional space mapping, and output inflammation types and overall micro-ecological evaluation.

According to one embodiment of the present invention, when determining whether the bacteria are Gram-positive through Gram staining, analysis of a Gram-stained slide image enables automation of test results, including Gram-positivity determination. To this end, in particular, one embodiment of the present invention provides an image analysis method for a Gram-stained slide, the method comprising: a Gram staining step (S110) of Gram-staining a specimen slide; a low-magnification imaging step (S120) of imaging an ideal zone of the stained specimen slide by using a low-magnification imaging unit, thereby obtaining a low-magnification image; an object detecting step (S130) of detecting an object of interest from the obtained low-magnification image and identifying the location of the object of interest; a high-magnification imaging step (S140) of imaging, at the identified location of the object of interest, an object region including the detected object of interest by using a high-magnification imaging unit, thereby obtaining a high-magnification image; a first classification step (S150) of classifying, by a bacteria classification unit, bacteria among the objects to be observed as Gram-positive bacteria and Gram-negative bacteria on the basis of the high-magnification image; and a second classification step (S160) of classifying, by the bacteria classification unit, bacteria among the detected objects to be observed as at least one of cocci, bacilli, and spirilla.

The application provides a method for improving stability of lactobacillus plantarum, and relates to the field of lactobacillus plantarum preparation process. The process steps of the method for improving stability of lactobacillus plantarum are strain screening, candidate strain separation from traditional fermented food pickles, placing the pickle sample into a dilution tank, smearing the diluted pickle sample on MRS solid culture medium, placing the MRS solid culture medium into an anaerobic incubator, adjusting to 36 DEG C, anaerobic culture for 36 hours, picking single colonies for gram staining and peroxidase test to identify lactic acid bacteria, and placing the bacterial liquid in MRS liquid culture medium with pH 3.0 and standing for 3 hours to screen strains with a survival rate greater than 85%. The application utilizes the electrostatic adsorption principle of chitosan-sodium alginate to construct a pH-responsive microcapsule, the capsule shell is quickly swollen and releases the strain in an acidic environment, and the capsule shell remains intact in an alkaline environment, so that the colonization rate of the strain in simulated gastric and intestinal fluid is increased by 70%.

The present invention belongs to the technical field of microbiology, and specifically relates to a strain Cd2 and an application thereof. A strain Cd2, the taxonomic name of the strain Cd2 is Pseudomonas aeruginosa, and the deposit number is GDMCC No.64110. The present invention identifies the screened strains by morphological observation, Gram staining and 16S rRNA sequence sequencing, and determines the inhibitory effect of the strains on the crucifer black rot fungus and the fruit cane mildew fungus Pseudomonas spores, thereby obtaining a strain Cd2, which has a strong inhibitory effect on the crucifer black rot fungus and the fruit cane mildew fungus Pseudomonas spores, and brings broad application prospects to the field of biological control of plant diseases caused by the crucifer black rot fungus and the fruit cane mildew disease.

The invention encompasses an integrated microbiological decision support and automation system that automatically classifies Gram staining and morphological analysis results obtained from positive blood culture and direct body fluid samples using artificial intelligence-based image processing algorithms, and utilizes this classification data as closed-loop decision input for dynamic medium selection, robotic inoculation guidance, and laboratory management.

The application relates to a new pesticide-degrading strain, in particular to a strain New bacteria Acinetobacter oryzae F9-1 . A strain F9-1 is obtained by separation, screening and identification from a soil sample in Hami City of Xinjiang Uygur Autonomous Region New bacteria Acinetobacter oryzae F9- 1 The strain is subjected to physiological and biochemical characteristic detection, the strain F9-1 shows small colonies, smooth surface, white semi-transparency on NB plates, and the results of gram staining, contact enzyme and indole experiment are positive, and the results of oxidase, V.P test and urease are negative. The strain F9-1 can hydrolyze hydrogen peroxide, can utilize L-alanine, lactic acid, L-aspartic acid and quinine acid, is sensitive to 8% NaCl, lincomycin, naphthylidine ketone acid and chloromycetin, and can degrade fluazifop-butyl and pendimethalin, and has the advantages of environmental protection, high efficiency, economy and sustainability, and is an effective means for solving the problem of pesticide pollution.

The invention relates to a tetracycline-resistant pseudomonas aeruginosa denitrifying bacterium, and belongs to the technical field of sewage treatment, the tetracycline-resistant pseudomonas aeruginosa denitrifying bacterium specifically comprises an anaerobic denitrification drug-resistant strain, the strain of the strain is pseudomonas aeruginosa, the name of the anaerobic denitrification drug-resistant strain is Pseudomonas aeruginosa YF2024, and the preservation number of the anaerobic denitrification drug-resistant strain is CGMCC NO.34912; the anaerobic denitrification drug-resistant strain is in the form of a short rod, the length is 1.0-1.5 mu m, the diameter is 0.6-0.7 mu m, gram staining is negative, VP reaction is positive, and drug sensitivity to tetracycline is negative. The denitrifying bacterium provided by the invention can still keep the nitrate nitrogen degradation rate of 98% under the conditions of high tetracycline and high nitrogen source.

The invention discloses a rapid screening method of cholesterol-lowering lactic acid bacteria, and belongs to the technical field of biology. Comprising the following steps: (1) coating a fermented food sample solution into an MRS-maize germ oil solid culture medium for preliminary screening culture, and screening single colonies with acid producing circles; (2) carrying out catalase inspection and gram staining microscopy on the strains obtained by primary screening, and screening out bacterial colonies with positive gram staining results and negative catalase experimental results; and (3) inoculating the primarily screened strain into a liquid culture medium containing cholesterol micelles for secondary screening culture, measuring the cholesterol concentration before and after culture, calculating the degradation rate, and selecting the strain with the strongest cholesterol degradation capability as a target strain. According to the method, in the screening process of the cholesterol-reducing lactic acid bacteria, the lactic acid bacteria can be effectively screened, experimental steps are simplified, and the cholesterol-reducing lactic acid bacteria can be primarily screened, so that the overall screening efficiency is improved.

An apparatus configured to process a sample to detect a pathogen receives a slide with the sample on the slide, in which the sample has been stained with one or more of a Gram stain, an Acid-Fast stain, or a Giemsa stain. An area of at least 5 mm2 of the sample is imaged at a rate of at least 15 mm2 per minute and a resolution of 0.3 pm or better to generate one or more images. The one or more images of the sample are processed with a classifier configured to detect the pathogen in the sample. In some embodiments, a plurality of cultured and stained samples on a plurality of slides are imaged at the resolution and processed with the classifier, which can increase the area processed and analyzed in order to decrease the culture time and corresponding time to diagnose the patient.

The invention belongs to the technical field of medical examination technology auxiliary equipment, and particularly relates to an automatic Gram dyeing machine with a 3D printing frame structure, the main structure is customized through 3D modeling software and printed through a fused deposition modeling (FDM) 3D printer, and the automatic Gram dyeing machine comprises a main body frame, a glass slide fixing frame, a silicone tube fixing plate and a switch cover plate; a steering engine fixing clamping position is formed in the main body frame; the steering engine fixing clamping position is used for installing a steering engine; a glass slide fixing frame is mounted on the steering engine; a dyeing tank is arranged in the main body frame; a waste liquid hole communicated with the dyeing tank is formed in one side of the main body frame; according to the invention, the rapid customized production of the equipment framework is realized, the research and development and manufacturing cost is reduced, the assembly and maintenance convenience of the functional module is optimized, the manpower is saved by the automatic process of gram dyeing, the potential health risk of gram dyeing liquor to human bodies is reduced, and the consistency and accuracy of dyeing results are improved.

The invention belongs to the technical field of microbiological inspection instruments, and particularly relates to a rotary gram staining device which comprises a supporting assembly, the slide placing part comprises a slide mounting bin and a slide mounting rack; the slide mounting rack is arranged in the slide mounting bin; the slide mounting bin is hinged to the supporting assembly; one end of the dyeing system is inserted into the top of the supporting assembly; the dyeing system comprises a rotating test bed and a rotating unit; a drying part, a primary dyeing part, a mordant dyeing part, a decoloring part, a redyeing part and a washing part are arranged on the side wall of the rotary test bed; the rotating unit drives the rotating test bed to rotate under the adjustment of the control device, so that one of the drying part, the primary dyeing part, the mordant dyeing part, the decolorizing part, the redyeing part and the washing part is aligned with the slide placing part.