35 results about "Gram staining" patented technology

The invention relates to a strain of Chryseobacterium sp. ZJUHULYY1 and application of the Chryseobacterium sp. ZJUHULYY1 in promotion of corn growth and iron absorption, and provides a strain of Chryseobacterium sp. ZJUHULYY1, which is preserved in China Center for Type Culture Collection on April 27, 2025, and has a preservation number of CCTCC NO: M 2025904. The strain of Chryseobacterium sp. ZJUHULYY1 is obtained by separating and purifying from rhizosphere soil of corn, and the strain of Chryseobacterium sp. ZJUHULYY1 is obtained by separating and purifying from rhizosphere soil of corn. The biological characteristics of the strain are as follows: after being cultured in an LB plate culture medium at 30 DEG C for 24 hours, the bacterial colony is round or oval, golden yellow, smooth and glossy in surface, neat in edge and negative in gram staining; when the strain is inoculated to rhizosphere of corn growing in calcareous soil, compared with a control group, the plant height, chlorophyll content, biomass and active iron content of the corn are all remarkably improved, the growth condition of the corn in the calcareous soil is effectively improved, and the absorption and utilization efficiency of the corn on iron elements is improved.

The invention belongs to the technical field of microbiology, and particularly relates to application of a high-cadmium-resistance strain Cd2 in promoting repair of heavy metal pollution of aster katsumadai. The invention relates to a strain Cd2 with high cadmium resistance, the taxonomic name of the strain Cd2 is Pseudomonas aeruginosa, and the preservation number of the strain Cd2 is GDMCC No.64110. The strain Cd2 has the advantages that the strain Cd2 has high cadmium resistance; according to the invention, screened strains are identified through morphological observation, gram staining and 16S rRNA (ribosomal Ribonucleic Acid) sequence sequencing, and a high-cadmium-tolerance strain Cd2 is inoculated into soil during the growth period of a hyperaccumulator Aster brachium, so that the influence of Cd2 on the growth and enrichment of the hyperaccumulator Aster brachium is researched. The result shows that when the high-cadmium-tolerance strain Cd2 is inoculated into the soil for planting the aster katsumadai, the cadmium absorption amount of the underground part and the overground part of the aster katsumadai and the cadmium accumulation amount of the whole plant are remarkably increased, and a technical support can be provided for strengthening hyperaccumulation plant remediation.

The invention belongs to the field of biological quarantine, and particularly relates to a method for separating and identifying pigeon-derived liver escherichia coli. Escherichia coli separation and identification are carried out on disease samples from Guangdong, Guangxi, Guizhou, Hainan and the like, and the epidemicity of the disease samples is determined; collecting livers of acute dead young meat pigeons, and separating strains; carrying out gram staining, physiological and biochemical identification and molecular identification on the separated strain; the growth and reproduction cycle and hemolysis characteristics of the strain are detected, 76 Escherichia coli infection occurs in 113 disease samples, and the infection rate is 67%. According to the method, laboratory diagnosis of infectious diseases of the meat pigeons is systematically completed through sample collection and separation, culture and identification of strains, it is determined that the young meat pigeons are infected by escherichia coli, and a foundation is laid for subsequent antibiotic-free green breeding of the meat pigeons resisting escherichia coli infection.

The invention relates to the field of medical detection, in particular to a fluorescent sensing composition, a fluorescent sensing film and application thereof. According to the invention, by screening a plurality of fluorescent indicators and combinations thereof, an indicator formula with simple components and low cost is finally obtained. Based on the formula, by optimizing the preparation process, the high-efficiency fluorescent sensing film resistant to damp-heat sterilization is developed. After a microorganism culture bottle prepared on the basis of the membrane is inoculated with Gram-negative bacilli, positive cocci and yeast fungi representative quality control strains, experiments of full-automatic microorganism culture system fluorescence curve determination, Gram staining, microorganism mass spectrum identification and the like prove that the fluorescence sensing membrane is excellent in detection performance. The fluorescent sensing film provided by the research is applied to clinical microorganism detection, can provide technical support for blood flow infection diagnosis, and is of great significance in suppressing antibiotic abuse and improving prognosis of blood flow infection patients.

The application discloses a triazine herbicide propoxur degrading bacterial strain and application thereof, the bacterial strain is SWFU-DH02 strain which is negative in gram staining reaction and is identified as Microbacterium sp. Exiguobacteruim sp. ) on November 22, 2021, and is deposited in the China General Microbiological Culture Collection Center, and the strain preservation number is CGMCC No. 23948. The application discloses, for the first time, the degradation effect of Microbacterium sp. Exiguobacteruim sp. ) on triazine herbicide propoxur, and a Microbacterium sp. high-efficiency degrading bacterium SWFU-DH02 is screened, the degradation efficiency of the bacterium on propoxur can reach 75.08%, and the bacterium grows optimally under the conditions of pH 7.0, temperature 28 DEG C and salt concentration 1%. The degrading bacterial strain SWFU-DH02 has the characteristics of simple culture, rapid growth and high efficiency of degradation. The application can solve the pollution problem of triazine herbicide propoxur in soil or water, prevent the phytotoxicity of herbicide residues on subsequent crops, and produce non-toxic and non-polluting green agricultural products. The application provides a new biological approach for modern pesticide pollution control.

The invention provides a bacillus high-yield strain for producing riboflavin and application of the bacillus high-yield strain, and belongs to the technical field of microbial fermentation. The bacillus strain is named as VB2-M-230602-66, the preservation unit is China Center for Type Culture Collection, the preservation date is December 1, 2023, and the preservation number is CCTCC NO: M20232421; according to the invention, the strain VB2-M-230602-66 with stable character is obtained by adopting physical mutagenesis separation and combining with shake flask screening of tolerance dose of a resistance marker, riboflavin can be produced through liquid fermentation, and the yield is stable; the bacterial strain has the biological characteristics that the bacterial strain is gram-positive bacteria through gram staining, the bacteria are cultured at 37 DEG C for 18 hours, the thalli are rod-shaped, 0.5-0.8 mu m * 2.0-5.4 mu m and are arranged in a single or paired manner, and bacterial colonies cultured on a solid culture medium are pale yellow, nearly circular, wet in surface, opaque and irregular in edge; the bacillus strain VB2-M-230602-66 provided by the invention is suitable for fermentation production of riboflavin (vitamin B2), has stable production performance, can obtain greater economic benefits, and is worthy of popularization.

The invention discloses a landfill leachate degrading bacterium S14 and belongs to the technical field of microorganisms, the landfill leachate degrading bacterium is bacillus subtilis and is preserved in the China Center for Type Culture Collection, the address of the preservation unit is Wuhan University, Wuchang District, Wuhan City, Hubei Province, and the preservation number is CCTCC M 2025283. Preferably, the bacterial colony is grey white, approximately circular and opaque, and wrinkles are formed in the middle of the bacterial colony. Preferably, gram staining is positive, and thalli are slender and rod-shaped. According to the invention, the S14 strain can effectively degrade main pollutants in the landfill leachate, including COD (Chemical Oxygen Demand), ammonia nitrogen and total phosphorus. In an experiment, the removal rates of the S14 strain on ammonia nitrogen, COD (Chemical Oxygen Demand) and total phosphorus respectively reach 88.84%, 55.88% and 82.68%.

The utility model provides a sterilization glass slide convenient for gram staining and identification, which comprises a glass slide main body, a sample information marking area, a standard bacterium area and a sample bacterium area are sequentially arranged on the front surface of the glass slide main body, and the sterilization glass slide is characterized in that the sample information marking area, the standard bacterium area and the sample bacterium area are sequentially arranged along the length direction of the glass slide main body; the front surface of the glass slide main body is partitioned through partition straight lines coated with the front surface of the glass slide main body, the partition straight lines are arranged in the width direction of the glass slide main body, and the multiple partition straight lines are sequentially distributed at intervals in the length direction of the glass slide main body; separation round lines are respectively arranged in the standard bacterium area and the sample bacterium area; and the thicknesses d of the separation straight lines and the separation round lines are 0.5-2 mm, and the widths D of the separation straight lines and the separation round lines are 0.5-2 mm. According to the utility model, the cross contamination problem in the staining process can be effectively improved, false positive and false negative phenomena are avoided, the accuracy of a staining result is improved, and the operation convenience, the result accuracy and the test efficiency of gram staining and identification of sample bacteria are improved.

The invention discloses bacillus velezensis Jcl-10 and application of the bacillus velezensis Jcl-10 in prevention and treatment of vibrio cholerae. The strain Jcl-10 is preserved in the China Center for Type Culture Collection, the preservation time is January 25, 2024, and the preservation number is CCTCC N: M 2024212. The bacillus velezensis Jcl-10 disclosed by the invention is identified as a gram positive bacterium after being subjected to gram staining. The bacterial strain can produce antibacterial peptide substances, antibacterial protein substances, polyketide synthetase substances and the like with bacteriostatic action, and can be well applied to prevention and treatment of aquatic diseases caused by vibrio cholerae. The Jcl-10 is added into the aquatic feed, so that the morbidity of aquaculture animals can be effectively reduced, and the economic yield of the aquaculture animals can be increased. In conclusion, the bacillus velezensis Jcl-10 disclosed by the invention has outstanding performance in the prevention and treatment of vibrio cholerae, and provides a powerful guarantee for continuous and healthy culture of aquatic products.

The invention relates to a novel bacterial strain capable of degrading pesticides, in particular to a novel bacterial strain Acinetobacter oryzae F9-1, and a preparation method of the novel bacterial strain Acinetobacter oryzae F9-1. A new strain Acinetobacter oryzae F9-1 is obtained by separation, screening and identification from a soil sample in Hami region of Xinjiang, the strain is subjected to physiological and biochemical characteristic detection, the new strain F9-1 shows that the bacterial colony is small, the surface is smooth, the new strain F9-1 is white and semitransparent on an NB plate, the results of Gram staining, catalase and indole experiments and the like are positive, and the results of oxidase, V.P tests, urease and the like are negative. The strain F9-1 can hydrolyze hydrogen peroxide; l-alanine, lactic acid, L-aspartic acid, quinic acid and the like can be utilized, the strain is sensitive to 8% NaCl, lincomycin, nalidixic acid, chloramphenicol and the like, fluridone and pendimethalin can be degraded, and the strain has the advantages of being environmentally friendly, efficient, economical, sustainable and the like and is an effective means for solving the pesticide pollution problem.

The present invention generally relates to improved methods for characterizing the cell wall complex composition of a bacterial specimen via differential transport kinetics of a small molecule. The present invention also relates to improved and automated methods for Gram differentiation of a bacterial specimen using nonlinear optical techniques.

The invention discloses pediococcus acidilactici capable of degrading nitrite and application of the pediococcus acidilactici capable of degrading nitrite. The pediococcus acidilactici capable of degrading nitrite is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.33428. Gram staining of the pediococcus acidilactici is positive, catalase contact test is negative, a 16S rDNA sequence is shown as SEQ ID NO: 1, the nitrite degradation rate of the pediococcus acidilactici is larger than 95%, and the pediococcus acidilactici grows well under the salt concentration of 4% or above. The invention further discloses an application of the nitrite degrading pediococcus acidilactici Pac.G1 in preparation of low-nitrite brassica campestris. Compared with the prior art, the brassica campestris prepared by applying the pediococcus acidilactici capable of degrading nitrite, disclosed by the invention, has the advantages that the nitrite content is obviously reduced, the chewiness, the vitamin C content, the total acid content and the delicious amino acid content of the product are higher, the quality, the taste and the flavor are better, and safer and healthier pickled vegetable products are provided for consumers.

The application discloses a gynecological micro-ecological detection system based on AI multi-modal fusion, and belongs to the technical field of gynecological micro-ecological detection. The system comprises a data acquisition module, a standardization module and a micro-ecological output unit. The data acquisition module acquires immunofluorescence signals, gram staining morphological characteristics and dry chemical indexes. The data acquisition module is connected to the standardization module. The standardization module comprises a pre-processing unit for pre-processing collected data. The standardization module is connected with a data classification unit for data classification. The data classification unit is connected to a heterologous data normalization processing unit. The gynecological micro-ecological detection system based on AI multi-modal fusion can realize accurate evaluation of gynecological micro-ecology, construct a dynamic model of microbial community parameters, realize inflammation process quantification, generate a visual micro-ecological atlas through three-dimensional space mapping, and output inflammation types and overall micro-ecological evaluation.

According to one embodiment of the present invention, when determining whether the bacteria are Gram-positive through Gram staining, analysis of a Gram-stained slide image enables automation of test results, including Gram-positivity determination. To this end, in particular, one embodiment of the present invention provides an image analysis method for a Gram-stained slide, the method comprising: a Gram staining step (S110) of Gram-staining a specimen slide; a low-magnification imaging step (S120) of imaging an ideal zone of the stained specimen slide by using a low-magnification imaging unit, thereby obtaining a low-magnification image; an object detecting step (S130) of detecting an object of interest from the obtained low-magnification image and identifying the location of the object of interest; a high-magnification imaging step (S140) of imaging, at the identified location of the object of interest, an object region including the detected object of interest by using a high-magnification imaging unit, thereby obtaining a high-magnification image; a first classification step (S150) of classifying, by a bacteria classification unit, bacteria among the objects to be observed as Gram-positive bacteria and Gram-negative bacteria on the basis of the high-magnification image; and a second classification step (S160) of classifying, by the bacteria classification unit, bacteria among the detected objects to be observed as at least one of cocci, bacilli, and spirilla.

The application provides a method for improving stability of lactobacillus plantarum, and relates to the field of lactobacillus plantarum preparation process. The process steps of the method for improving stability of lactobacillus plantarum are strain screening, candidate strain separation from traditional fermented food pickles, placing the pickle sample into a dilution tank, smearing the diluted pickle sample on MRS solid culture medium, placing the MRS solid culture medium into an anaerobic incubator, adjusting to 36 DEG C, anaerobic culture for 36 hours, picking single colonies for gram staining and peroxidase test to identify lactic acid bacteria, and placing the bacterial liquid in MRS liquid culture medium with pH 3.0 and standing for 3 hours to screen strains with a survival rate greater than 85%. The application utilizes the electrostatic adsorption principle of chitosan-sodium alginate to construct a pH-responsive microcapsule, the capsule shell is quickly swollen and releases the strain in an acidic environment, and the capsule shell remains intact in an alkaline environment, so that the colonization rate of the strain in simulated gastric and intestinal fluid is increased by 70%.

The application relates to a new pesticide-degrading strain, in particular to a strain New bacteria Acinetobacter oryzae F9-1 . A strain F9-1 is obtained by separation, screening and identification from a soil sample in Hami City of Xinjiang Uygur Autonomous Region New bacteria Acinetobacter oryzae F9- 1 The strain is subjected to physiological and biochemical characteristic detection, the strain F9-1 shows small colonies, smooth surface, white semi-transparency on NB plates, and the results of gram staining, contact enzyme and indole experiment are positive, and the results of oxidase, V.P test and urease are negative. The strain F9-1 can hydrolyze hydrogen peroxide, can utilize L-alanine, lactic acid, L-aspartic acid and quinine acid, is sensitive to 8% NaCl, lincomycin, naphthylidine ketone acid and chloromycetin, and can degrade fluazifop-butyl and pendimethalin, and has the advantages of environmental protection, high efficiency, economy and sustainability, and is an effective means for solving the problem of pesticide pollution.

The invention relates to a tetracycline-resistant pseudomonas aeruginosa denitrifying bacterium, and belongs to the technical field of sewage treatment, the tetracycline-resistant pseudomonas aeruginosa denitrifying bacterium specifically comprises an anaerobic denitrification drug-resistant strain, the strain of the strain is pseudomonas aeruginosa, the name of the anaerobic denitrification drug-resistant strain is Pseudomonas aeruginosa YF2024, and the preservation number of the anaerobic denitrification drug-resistant strain is CGMCC NO.34912; the anaerobic denitrification drug-resistant strain is in the form of a short rod, the length is 1.0-1.5 mu m, the diameter is 0.6-0.7 mu m, gram staining is negative, VP reaction is positive, and drug sensitivity to tetracycline is negative. The denitrifying bacterium provided by the invention can still keep the nitrate nitrogen degradation rate of 98% under the conditions of high tetracycline and high nitrogen source.

The invention discloses a rapid screening method of cholesterol-lowering lactic acid bacteria, and belongs to the technical field of biology. Comprising the following steps: (1) coating a fermented food sample solution into an MRS-maize germ oil solid culture medium for preliminary screening culture, and screening single colonies with acid producing circles; (2) carrying out catalase inspection and gram staining microscopy on the strains obtained by primary screening, and screening out bacterial colonies with positive gram staining results and negative catalase experimental results; and (3) inoculating the primarily screened strain into a liquid culture medium containing cholesterol micelles for secondary screening culture, measuring the cholesterol concentration before and after culture, calculating the degradation rate, and selecting the strain with the strongest cholesterol degradation capability as a target strain. According to the method, in the screening process of the cholesterol-reducing lactic acid bacteria, the lactic acid bacteria can be effectively screened, experimental steps are simplified, and the cholesterol-reducing lactic acid bacteria can be primarily screened, so that the overall screening efficiency is improved.

The invention belongs to the technical field of medical examination technology auxiliary equipment, and particularly relates to an automatic Gram dyeing machine with a 3D printing frame structure, the main structure is customized through 3D modeling software and printed through a fused deposition modeling (FDM) 3D printer, and the automatic Gram dyeing machine comprises a main body frame, a glass slide fixing frame, a silicone tube fixing plate and a switch cover plate; a steering engine fixing clamping position is formed in the main body frame; the steering engine fixing clamping position is used for installing a steering engine; a glass slide fixing frame is mounted on the steering engine; a dyeing tank is arranged in the main body frame; a waste liquid hole communicated with the dyeing tank is formed in one side of the main body frame; according to the invention, the rapid customized production of the equipment framework is realized, the research and development and manufacturing cost is reduced, the assembly and maintenance convenience of the functional module is optimized, the manpower is saved by the automatic process of gram dyeing, the potential health risk of gram dyeing liquor to human bodies is reduced, and the consistency and accuracy of dyeing results are improved.

The invention belongs to the technical field of microbiological inspection instruments, and particularly relates to a rotary gram staining device which comprises a supporting assembly, the slide placing part comprises a slide mounting bin and a slide mounting rack; the slide mounting rack is arranged in the slide mounting bin; the slide mounting bin is hinged to the supporting assembly; one end of the dyeing system is inserted into the top of the supporting assembly; the dyeing system comprises a rotating test bed and a rotating unit; a drying part, a primary dyeing part, a mordant dyeing part, a decoloring part, a redyeing part and a washing part are arranged on the side wall of the rotary test bed; the rotating unit drives the rotating test bed to rotate under the adjustment of the control device, so that one of the drying part, the primary dyeing part, the mordant dyeing part, the decolorizing part, the redyeing part and the washing part is aligned with the slide placing part.

The utility model belongs to the technical field of tool boxes, and particularly discloses a bacteria separation and identification tool box which comprises a box body, a top cover is arranged at the top of the box body, a mounting groove is formed in the bottom of the top cover, the surface of the box body is connected with the interior of the mounting groove in a sliding mode, two symmetrical rotating shafts are fixedly connected to the outer surface of the top cover, and the rotating shafts are arranged on the top cover. A handle is arranged on the outer surface of the top cover, the cross section of the handle is in a [shape, a fixing block is fixedly connected to the outer surface of the box body, and an arc surface is arranged at the bottom of the fixing block. Nutrient agar powder, a glass slide, a gram staining solution, a washing bottle, a disposable sterile plate and other tools can be placed in the box body through the two first partition plates, a worker enables a two-way threaded rod to rotate, and the two-way threaded rod can drive the two first partition plates to move relatively through two sections of opposite threads on the surface of the two-way threaded rod; and the space in the box body is adjusted.

The application discloses a vaginal micro-ecological detection staining method, mainly comprising the following steps: 1) taking female genital tract secretion and diluting the same by a diluent to form a suspension; 2) coating the suspension on a glass slide; 3) drying and fixing the suspension on the glass slide; 4) immersing the glass slide in a eosin dye solution for staining, and then taking out the glass slide and washing the same by flowing water; 5) immersing the glass slide in a methylene blue dye solution for staining, and then taking out the glass slide and washing the same by flowing water; 6) performing gram staining on the glass slide; and 7) observing by using a microscope. The staining method can not only clearly show the trophozoite shape and flagellum shape of Trichomonas vaginalis, but also accurately distinguish leukocytes and trichomonads, is favorable to improving the accuracy of trichomonas vaginalis detection, and is favorable to comprehensively and accurately detecting the vaginal micro-ecology.

The invention discloses antioxidant lactobacillus pentosus and application thereof, the antioxidant lactobacillus pentosus Lpe.C1 is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.33427. Gram staining of the lactobacillus pentosus Lpe.C1 is positive, catalase contact test is negative, a 16S rDNA sequence is shown as SEQ ID NO: 1, the hydroxyl free radical scavenging capacity of the lactobacillus pentosus Lpe.C1 is larger than 65%, the DPPH free radical scavenging capacity of the lactobacillus pentosus Lpe.C1 is larger than 45%, and the lactobacillus pentosus Lpe.C1 grows well under the salt concentration of 4% or above. The application of the antioxidant lactobacillus pentosus comprises the step of preparing antioxidant pickled cabbage type brassica campestris and pickled Chinese cabbage type brassica campestris by utilizing the separated and purified antioxidant lactobacillus pentosus Lpe.C1. According to the pickled brassica campestris prepared by applying the antioxidant lactobacillus pentosus Lpe.C1 disclosed by the invention, the oxidation resistance of a product is remarkably improved, the vitamin C content, the delicate flavor, the sweet amino acid content and the chewiness of the product are higher, and the quality, the mouth feel and the flavor of the product are better.

The present application relates to the field of medical detection, in particular to a fluorescence sensing composition, a fluorescence sensing film and application thereof. Through screening a variety of fluorescence indicators and combinations thereof, a simple and cost-effective indicator formula is finally obtained. Based on the formula, through optimizing the preparation process, a high-efficiency fluorescence sensing film resistant to moist heat sterilization is developed. After inoculating gram-negative bacilli, gram-positive cocci and yeast-type fungi representative quality control strains into the microorganism culture bottle prepared based on the film, through fluorescence curve determination of the full-automatic microorganism culture system, gram staining, microorganism mass spectrum identification and other experiments, it is proved that the fluorescence sensing film has excellent detection performance. The fluorescence sensing film proposed in the present application can be applied to the detection of clinical microorganisms, which can provide technical support for the diagnosis of bloodstream infections, and has important significance for curbing the abuse of antibiotics and improving the prognosis of patients with bloodstream infections.

The invention discloses a gynecological micro-ecology detection system based on AI multi-modal fusion, and belongs to the technical field of gynecological micro-ecology detection. Comprising a data acquisition module, a standardization module and a micro-ecological output unit, wherein the data acquisition module is used for acquiring an immunofluorescence signal, gram staining morphological characteristics and dry chemical indexes; the data acquisition module is connected to the standardization module, and the standardization module comprises a pre-processing unit for pre-processing acquired data; the data classification unit is connected with the pre-processing unit and used for data classification, and the data classification unit is connected to the heterologous data normalization processing unit. According to the gynecological micro-ecology detection system based on AI multi-modal fusion, precise evaluation of gynecological micro-ecology can be achieved, a dynamic model of microbial community parameters is constructed, and the detection accuracy is improved. The inflammation process quantification is realized; a visual micro-ecological map is generated through three-dimensional space mapping, and inflammation types and micro-ecological overall evaluation are output.

The centrifugal gram dyeing spray dyeing instrument comprises a machine body and a spray dyeing assembly, the spray dyeing assembly is located on one side of the machine body and used for dyeing microbial glass slides, an outer barrel is fixedly connected to the top of the machine body, a top cover assembly is arranged at the top of the outer barrel, and a drying assembly is arranged on the surface of the outer barrel. The outer barrel is used for drying the microbial glass slides, and a centrifugal mechanism is arranged in an inner cavity of the outer barrel. According to the centrifugal gram dyeing spray dyeing instrument, the fixing frame of the centrifugal mechanism is matched with the press-fitting assembly, a rubber cushion can firmly fix a glass slide, displacement or specimen scratching during centrifugal rotation is avoided, meanwhile, the top cover assembly is of a press-type locking structure and is combined with a transparent observation window, the specimen can be conveniently and rapidly loaded and unloaded, and the dyeing process can be monitored in real time; meanwhile, the closed outer barrel reduces dye liquor volatilization, and the operation safety is improved.

The application discloses a gram staining box, which is characterized by four independent containing cavities for preloading corresponding dyeing liquid separated by a partition plate in the box body, a soft film with a partitioned tearable sealing film and a corresponding jack for each containing cavity, and is integrated into a one-time staining box. The slide is inserted into each containing cavity through the jack to complete step-by-step staining. The gram staining box integrates four kinds of dyeing liquids required for gram staining into a single box body, stores the dyeing liquids in a closed mode, and is double-sealed by the soft film and the partitioned tearable sealing film. Only a single and corresponding jack is opened step by step for the staining operation, the area of the dyeing liquid exposed to the air is greatly reduced, the leakage of volatile and irritating gas of the dyeing liquid is inhibited from the source, harmful gas is prevented from accumulating in the closed microbiological laboratory, the occupational injury of the respiratory tract and the body of the experimental personnel caused by the chemical volatile substances is reduced, the experimental operation environment is optimized, and the operation safety is improved.

The present invention belongs to the field of microbiology and specifically relates to a strain G7 and its application. The strain G7 has the taxonomic name Bacillus subtilis and the deposit number GDMCC No. 63938. The present invention identifies the selected strain through morphological observation, Gram staining, and 16S rRNA sequencing, and measures its inhibitory effect on the cane mildew pathogen Arthrotrichum spp., resulting in a strain G7 that exhibits a strong inhibitory effect against the cane mildew pathogen Arthrotrichum spp., offering broad application prospects in the field of biological control of cane mildew diseases.

1. Name of the product in this design: Fully Automatic Dyeing Machine (GA32). 2. Intended use of this design: for acid-fast staining, Gram staining, fluorescent staining and Wright staining of clinical samples. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1.

This invention discloses a method for identifying Gram-stained blood culture samples using microscopic images, belonging to the field of image processing technology. The invention acquires Gram-stained blood culture smear images under an optical microscope and annotates pathogenic bacteria using rectangular bounding boxes. Subsequently, the annotated image undergoes color space conversion to obtain an L-channel image and calculates the gradient magnitude of each pixel. After dividing the L-channel image into blocks, a dynamic shearing threshold is calculated based on the average gradient magnitude of each sub-block. The grayscale histogram of each sub-block is then sheared to obtain histogram-equalized sub-blocks. Next, the block effect of each equalized sub-block is eliminated, and it is concatenated with the A-channel and B-channel images and converted back to the RGB color space to obtain a contrast-enhanced image. Finally, the contrast-enhanced image is scaled and input into an enhanced YOLOv11 target detection network, ultimately achieving accurate identification of pathogenic bacteria categories. This invention effectively improves the identification accuracy of pathogenic bacteria in Gram-stained blood culture samples.