2 results about "Gram staining" patented technology

This invention discloses a method for identifying Gram-stained blood culture samples using microscopic images, belonging to the field of image processing technology. The invention acquires Gram-stained blood culture smear images under an optical microscope and annotates pathogenic bacteria using rectangular bounding boxes. Subsequently, the annotated image undergoes color space conversion to obtain an L-channel image and calculates the gradient magnitude of each pixel. After dividing the L-channel image into blocks, a dynamic shearing threshold is calculated based on the average gradient magnitude of each sub-block. The grayscale histogram of each sub-block is then sheared to obtain histogram-equalized sub-blocks. Next, the block effect of each equalized sub-block is eliminated, and it is concatenated with the A-channel and B-channel images and converted back to the RGB color space to obtain a contrast-enhanced image. Finally, the contrast-enhanced image is scaled and input into an enhanced YOLOv11 target detection network, ultimately achieving accurate identification of pathogenic bacteria categories. This invention effectively improves the identification accuracy of pathogenic bacteria in Gram-stained blood culture samples.