14 results about "Gram staining" patented technology

The invention provides a bacillus high-yield strain for producing riboflavin and application of the bacillus high-yield strain, and belongs to the technical field of microbial fermentation. The bacillus strain is named as VB2-M-230602-66, the preservation unit is China Center for Type Culture Collection, the preservation date is December 1, 2023, and the preservation number is CCTCC NO: M20232421; according to the invention, the strain VB2-M-230602-66 with stable character is obtained by adopting physical mutagenesis separation and combining with shake flask screening of tolerance dose of a resistance marker, riboflavin can be produced through liquid fermentation, and the yield is stable; the bacterial strain has the biological characteristics that the bacterial strain is gram-positive bacteria through gram staining, the bacteria are cultured at 37 DEG C for 18 hours, the thalli are rod-shaped, 0.5-0.8 mu m * 2.0-5.4 mu m and are arranged in a single or paired manner, and bacterial colonies cultured on a solid culture medium are pale yellow, nearly circular, wet in surface, opaque and irregular in edge; the bacillus strain VB2-M-230602-66 provided by the invention is suitable for fermentation production of riboflavin (vitamin B2), has stable production performance, can obtain greater economic benefits, and is worthy of popularization.

The utility model provides a sterilization glass slide convenient for gram staining and identification, which comprises a glass slide main body, a sample information marking area, a standard bacterium area and a sample bacterium area are sequentially arranged on the front surface of the glass slide main body, and the sterilization glass slide is characterized in that the sample information marking area, the standard bacterium area and the sample bacterium area are sequentially arranged along the length direction of the glass slide main body; the front surface of the glass slide main body is partitioned through partition straight lines coated with the front surface of the glass slide main body, the partition straight lines are arranged in the width direction of the glass slide main body, and the multiple partition straight lines are sequentially distributed at intervals in the length direction of the glass slide main body; separation round lines are respectively arranged in the standard bacterium area and the sample bacterium area; and the thicknesses d of the separation straight lines and the separation round lines are 0.5-2 mm, and the widths D of the separation straight lines and the separation round lines are 0.5-2 mm. According to the utility model, the cross contamination problem in the staining process can be effectively improved, false positive and false negative phenomena are avoided, the accuracy of a staining result is improved, and the operation convenience, the result accuracy and the test efficiency of gram staining and identification of sample bacteria are improved.

The invention relates to a novel bacterial strain capable of degrading pesticides, in particular to a novel bacterial strain Acinetobacter oryzae F9-1, and a preparation method of the novel bacterial strain Acinetobacter oryzae F9-1. A new strain Acinetobacter oryzae F9-1 is obtained by separation, screening and identification from a soil sample in Hami region of Xinjiang, the strain is subjected to physiological and biochemical characteristic detection, the new strain F9-1 shows that the bacterial colony is small, the surface is smooth, the new strain F9-1 is white and semitransparent on an NB plate, the results of Gram staining, catalase and indole experiments and the like are positive, and the results of oxidase, V.P tests, urease and the like are negative. The strain F9-1 can hydrolyze hydrogen peroxide; l-alanine, lactic acid, L-aspartic acid, quinic acid and the like can be utilized, the strain is sensitive to 8% NaCl, lincomycin, nalidixic acid, chloramphenicol and the like, fluridone and pendimethalin can be degraded, and the strain has the advantages of being environmentally friendly, efficient, economical, sustainable and the like and is an effective means for solving the pesticide pollution problem.

The present invention generally relates to improved methods for characterizing the cell wall complex composition of a bacterial specimen via differential transport kinetics of a small molecule. The present invention also relates to improved and automated methods for Gram differentiation of a bacterial specimen using nonlinear optical techniques.

The application discloses a gynecological micro-ecological detection system based on AI multi-modal fusion, and belongs to the technical field of gynecological micro-ecological detection. The system comprises a data acquisition module, a standardization module and a micro-ecological output unit. The data acquisition module acquires immunofluorescence signals, gram staining morphological characteristics and dry chemical indexes. The data acquisition module is connected to the standardization module. The standardization module comprises a pre-processing unit for pre-processing collected data. The standardization module is connected with a data classification unit for data classification. The data classification unit is connected to a heterologous data normalization processing unit. The gynecological micro-ecological detection system based on AI multi-modal fusion can realize accurate evaluation of gynecological micro-ecology, construct a dynamic model of microbial community parameters, realize inflammation process quantification, generate a visual micro-ecological atlas through three-dimensional space mapping, and output inflammation types and overall micro-ecological evaluation.

According to one embodiment of the present invention, when determining whether the bacteria are Gram-positive through Gram staining, analysis of a Gram-stained slide image enables automation of test results, including Gram-positivity determination. To this end, in particular, one embodiment of the present invention provides an image analysis method for a Gram-stained slide, the method comprising: a Gram staining step (S110) of Gram-staining a specimen slide; a low-magnification imaging step (S120) of imaging an ideal zone of the stained specimen slide by using a low-magnification imaging unit, thereby obtaining a low-magnification image; an object detecting step (S130) of detecting an object of interest from the obtained low-magnification image and identifying the location of the object of interest; a high-magnification imaging step (S140) of imaging, at the identified location of the object of interest, an object region including the detected object of interest by using a high-magnification imaging unit, thereby obtaining a high-magnification image; a first classification step (S150) of classifying, by a bacteria classification unit, bacteria among the objects to be observed as Gram-positive bacteria and Gram-negative bacteria on the basis of the high-magnification image; and a second classification step (S160) of classifying, by the bacteria classification unit, bacteria among the detected objects to be observed as at least one of cocci, bacilli, and spirilla.

The application provides a method for improving stability of lactobacillus plantarum, and relates to the field of lactobacillus plantarum preparation process. The process steps of the method for improving stability of lactobacillus plantarum are strain screening, candidate strain separation from traditional fermented food pickles, placing the pickle sample into a dilution tank, smearing the diluted pickle sample on MRS solid culture medium, placing the MRS solid culture medium into an anaerobic incubator, adjusting to 36 DEG C, anaerobic culture for 36 hours, picking single colonies for gram staining and peroxidase test to identify lactic acid bacteria, and placing the bacterial liquid in MRS liquid culture medium with pH 3.0 and standing for 3 hours to screen strains with a survival rate greater than 85%. The application utilizes the electrostatic adsorption principle of chitosan-sodium alginate to construct a pH-responsive microcapsule, the capsule shell is quickly swollen and releases the strain in an acidic environment, and the capsule shell remains intact in an alkaline environment, so that the colonization rate of the strain in simulated gastric and intestinal fluid is increased by 70%.

The invention belongs to the technical field of medical examination technology auxiliary equipment, and particularly relates to an automatic Gram dyeing machine with a 3D printing frame structure, the main structure is customized through 3D modeling software and printed through a fused deposition modeling (FDM) 3D printer, and the automatic Gram dyeing machine comprises a main body frame, a glass slide fixing frame, a silicone tube fixing plate and a switch cover plate; a steering engine fixing clamping position is formed in the main body frame; the steering engine fixing clamping position is used for installing a steering engine; a glass slide fixing frame is mounted on the steering engine; a dyeing tank is arranged in the main body frame; a waste liquid hole communicated with the dyeing tank is formed in one side of the main body frame; according to the invention, the rapid customized production of the equipment framework is realized, the research and development and manufacturing cost is reduced, the assembly and maintenance convenience of the functional module is optimized, the manpower is saved by the automatic process of gram dyeing, the potential health risk of gram dyeing liquor to human bodies is reduced, and the consistency and accuracy of dyeing results are improved.

The utility model belongs to the technical field of tool boxes, and particularly discloses a bacteria separation and identification tool box which comprises a box body, a top cover is arranged at the top of the box body, a mounting groove is formed in the bottom of the top cover, the surface of the box body is connected with the interior of the mounting groove in a sliding mode, two symmetrical rotating shafts are fixedly connected to the outer surface of the top cover, and the rotating shafts are arranged on the top cover. A handle is arranged on the outer surface of the top cover, the cross section of the handle is in a [shape, a fixing block is fixedly connected to the outer surface of the box body, and an arc surface is arranged at the bottom of the fixing block. Nutrient agar powder, a glass slide, a gram staining solution, a washing bottle, a disposable sterile plate and other tools can be placed in the box body through the two first partition plates, a worker enables a two-way threaded rod to rotate, and the two-way threaded rod can drive the two first partition plates to move relatively through two sections of opposite threads on the surface of the two-way threaded rod; and the space in the box body is adjusted.

The present application relates to the field of medical detection, in particular to a fluorescence sensing composition, a fluorescence sensing film and application thereof. Through screening a variety of fluorescence indicators and combinations thereof, a simple and cost-effective indicator formula is finally obtained. Based on the formula, through optimizing the preparation process, a high-efficiency fluorescence sensing film resistant to moist heat sterilization is developed. After inoculating gram-negative bacilli, gram-positive cocci and yeast-type fungi representative quality control strains into the microorganism culture bottle prepared based on the film, through fluorescence curve determination of the full-automatic microorganism culture system, gram staining, microorganism mass spectrum identification and other experiments, it is proved that the fluorescence sensing film has excellent detection performance. The fluorescence sensing film proposed in the present application can be applied to the detection of clinical microorganisms, which can provide technical support for the diagnosis of bloodstream infections, and has important significance for curbing the abuse of antibiotics and improving the prognosis of patients with bloodstream infections.

The centrifugal gram dyeing spray dyeing instrument comprises a machine body and a spray dyeing assembly, the spray dyeing assembly is located on one side of the machine body and used for dyeing microbial glass slides, an outer barrel is fixedly connected to the top of the machine body, a top cover assembly is arranged at the top of the outer barrel, and a drying assembly is arranged on the surface of the outer barrel. The outer barrel is used for drying the microbial glass slides, and a centrifugal mechanism is arranged in an inner cavity of the outer barrel. According to the centrifugal gram dyeing spray dyeing instrument, the fixing frame of the centrifugal mechanism is matched with the press-fitting assembly, a rubber cushion can firmly fix a glass slide, displacement or specimen scratching during centrifugal rotation is avoided, meanwhile, the top cover assembly is of a press-type locking structure and is combined with a transparent observation window, the specimen can be conveniently and rapidly loaded and unloaded, and the dyeing process can be monitored in real time; meanwhile, the closed outer barrel reduces dye liquor volatilization, and the operation safety is improved.

1. Name of the product in this design: Fully Automatic Dyeing Machine (GA32). 2. Intended use of this design: for acid-fast staining, Gram staining, fluorescent staining and Wright staining of clinical samples. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1.

This invention discloses a method for identifying Gram-stained blood culture samples using microscopic images, belonging to the field of image processing technology. The invention acquires Gram-stained blood culture smear images under an optical microscope and annotates pathogenic bacteria using rectangular bounding boxes. Subsequently, the annotated image undergoes color space conversion to obtain an L-channel image and calculates the gradient magnitude of each pixel. After dividing the L-channel image into blocks, a dynamic shearing threshold is calculated based on the average gradient magnitude of each sub-block. The grayscale histogram of each sub-block is then sheared to obtain histogram-equalized sub-blocks. Next, the block effect of each equalized sub-block is eliminated, and it is concatenated with the A-channel and B-channel images and converted back to the RGB color space to obtain a contrast-enhanced image. Finally, the contrast-enhanced image is scaled and input into an enhanced YOLOv11 target detection network, ultimately achieving accurate identification of pathogenic bacteria categories. This invention effectively improves the identification accuracy of pathogenic bacteria in Gram-stained blood culture samples.

The invention discloses bacillus velezensis and application thereof, the morphological characteristics of the bacillus velezensis CYFYC1-1 are as follows: after the bacillus velezensis CYFYC1-1 is cultured on an NA solid culture medium for 24 hours, a single colony is formed, and the colony is round, milky white, opaque, free of gloss and irregular in edge, and has wrinkles and bulges on the surface; through observation of a scanning electron microscope, thalli are rod-shaped and accord with typical morphological characteristics of bacillus; the Gram staining result is positive, spores can be generated, and the strain has high stress resistance. The compound has a remarkable inhibition effect on Didymela segeticola infecting tobacco, the hypha growth inhibition rate reaches 71.40%, interference to tobacco phyllosphere non-pathogenic bacteria is small, the drug resistance risk is low, and the tobacco leaf spot disease caused by the pathogenic bacteria can be effectively prevented and treated.