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Bromoxynil octanoate degrading bacteria and bacterial agent prepared from same

A technology of octanoyl bromoxynil and degrading bacteria, which is applied in the field of biotechnology, can solve the problems of pesticide pollution and environmental pollution, and achieve the problem of excessive pesticide octanoyl bromoxynil residues, protection of the ecological environment, and appropriate survival time Effect

Active Publication Date: 2011-01-05
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

Although bromoxynil has played a great role in the control of weeds in farmland, due to its large-scale use, it has caused serious environmental pollution problems, and it accumulates in animals, plants, and even humans through bioaccumulation and food chains. storage, the pollution problem of pesticides has emerged today

Method used

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  • Bromoxynil octanoate degrading bacteria and bacterial agent prepared from same
  • Bromoxynil octanoate degrading bacteria and bacterial agent prepared from same
  • Bromoxynil octanoate degrading bacteria and bacterial agent prepared from same

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Embodiment Construction

[0023] 1 Isolation and identification of strains

[0024] The bromoxynil-octanoyl enriched bacterial solution was taken for gradient dilution. The dilution is coated on the inorganic salt solid medium containing 300mg / L bromoxynil octanoyl (prescription is: every liter contains 1.5g K 2 HPO 4 , 0.5g KH 2 PO 4 , 0.2g MgSO 4 ·7H 2 O, 1.0gNaCl, 1.0g (NH 4 ) 2 SO 4 , 15g agar, pH 7.0), cultivated at 30°C for several days. Pick a single colony to verify its degradation effect, and save a strain with higher degradation efficiency for subsequent experiments. See the picture of its colony figure 1 . The strain was identified as Acinetobacter baumannii by 16SrDNA sequence analysis combined with the results of physiological and biochemical characteristics, and named: XB2. The main biological characteristic is G - , the bacteria are short rod-shaped, short rod-shaped, without spores, strictly easy to grow; oxidase negative; catalase positive; can grow with octanoyl bromoxyni...

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Abstract

The invention provides degrading bacteria for eliminating residue of pesticide, namely bromoxynil octanoate, and a degrading bacterial agent thereof, and belongs to the technical field of biology high-technology. The strain is Gram staining reaction negative bacteria XB2 and is identified as Acinetobacter baumannii. The strain has main biological characteristics that: the strain is G-; the thallus has a short-rod shape with the size of 0.9-1.6*1.5-2.5 mu m, has no spores and is strictly aerobic; and the strain is negative for oxidase and positive for catalase and can grow by taking the bromoxynil octanoate as a unique carbon source. The degrading bacteria is directly applied to make bromoxynil octanoate residue in crops reduced by over 90 percent, solves the problem that the bromoxynil octanoate residue in agricultural production is overproof, and can produce non-toxic and nuisanceless green agricultural products.

Description

1. Technical field [0001] The invention provides a degrading bacteria of bromoxynil octanoyl and the bacterial agent produced therefrom, belonging to the high-tech field of biology, using microorganisms to degrade chemical pesticide residues, and suitable for the production and processing of green and pollution-free agricultural products in modern agricultural production . 2. Background technology [0002] With the changes in agricultural planting structure and operation mode, as well as changes in agricultural cultivation methods, pesticides have gradually become an indispensable and important substance for high and stable agricultural yields. Their use has greatly promoted the development of modern agriculture, restored a large number of losses caused by diseases, insect pests and weeds for agricultural production, and greatly improved the labor efficiency and mechanization degree of agricultural production, and played a role in the modernization of agricultural production...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/04A62D101/28
Inventor 蔡天明陈立伟徐静王青玲
Owner NANJING AGRICULTURAL UNIVERSITY
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