The invention provides a colloidal electrolyte, comprising 0.3 wt%-0.6 wt% of nano-silica, 0.02 wt%-0.7 wt% of sodium silicate, 0.01 wt%-0.3 wt% of trisodium citrate, and the balance being a sulfuric acid water solution. The invention also provides a mixing apparatus for the colloidal electrolyte. The invention also provides a preparation method of the colloidal electrolyte. The colloidal electrolyte provided by the invention is a jelly-like full-gel colloid, can not produce sulfuric acid-leakage phenomenon when being used for a storage battery, enables the storage battery to have the advantages of large electric capacity, long discharge time, long maintaining time of high electric capacity, good large-current discharge performance, strong charge acceptance, wide adaptive ambient temperature, long service life, etc. Experiments show that the storage battery has good capacity, good discharge performance, good capacity-recovery performance after overdischarge, good cycle life and relatively low internal resistance, when the colloidal electrolyte provided by the invention is used for the storage battery.