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Strain of petroleum aromatic hydrocarbon degrading bacterium and applications thereof

A technology for aromatic hydrocarbons and degrading bacteria, applied in the field of microbial degradation of petroleum pollutants, to achieve high-efficiency and rapid degradation capabilities, good application prospects and economic value, and the effect of a wide range of temperatures

Active Publication Date: 2018-04-10
YANGTZE UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Microbial degradation has been proven to be a very effective oil pollution removal technology, which has the advantages of cost-effectiveness, environmental friendliness, and no secondary pollution. The key to this technology is to screen out microorganisms that can efficiently degrade target pollutants. Due to the poor tolerance of degrading microorganisms to the growth environment and degradation environment, there is a large difference in the degradation ability of crude oil. Therefore, it is necessary to screen crude oil aromatic hydrocarbon degrading bacteria that have low requirements on the growth environment, fast growth speed, and fast degradation speed. To study its degradation characteristics and provide bacterial strains and genetic materials for the biodegradation of aromatic hydrocarbon compounds polluted by crude oil

Method used

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  • Strain of petroleum aromatic hydrocarbon degrading bacterium and applications thereof
  • Strain of petroleum aromatic hydrocarbon degrading bacterium and applications thereof
  • Strain of petroleum aromatic hydrocarbon degrading bacterium and applications thereof

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Embodiment Construction

[0025] The present invention screens and confirms the bacterial strain type through the following methods, and provides various excellent properties of the bacterial strain at the same time, and the specific content is as follows:

[0026] 1 Materials and methods

[0027] 1.1 Collection of oil-contaminated soil samples

[0028] The oil-contaminated soil samples came from the oil-contaminated soil in the oil production area of ​​Qianjiang Guanghua Oilfield.

[0029] The moisture content of the polluted soil was determined to be 35%, and the pH value was 8.0.

[0030] 1.2 Medium

[0031] 1.2.1 Inorganic salt medium

[0032] NaNO 3 1.5 g, (NH 4 ) 2 SO 4 1.5 g, K 2 HPO 4 1.0 g, MgSO 4 ·7H 2 O 0.5 g, KCl 0.5 g, NaCl 5.0 g, FeSO 4 ·7H 2 O 0.01 g, CaCl 2 0.02 g, 1000 mL distilled water, pH7.0, 0.5% petroleum, 100 mL per Erlenmeyer flask.

[0033] 1.2.2 Bacterial selective medium

[0034] Beef extract 5.0 g, peptone 10.0 g, NaCl 5.0 g, agar 20 g, sterile nystatin 5...

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Abstract

The invention discloses a petroleum aromatic hydrocarbon degrading bacterium (G-40), which is named as brevibacillus laterosporus G-40 and belongs to brevibacillus. The strain has been saved in ChinaCenter for Type Culture Collection (CCTCC), Wuhan university, China in June 30, 2017. The preservation number is CCTCC No. M2017400. The strain is screened from petroleum polluted soil in the oil producing region of Guanghua oil field in Qianjiang city. The strain (G-40) has an ellipsoidal shape. The result of gram staining is positive. The strain (G-40) can degrade 53.02% of petroleum aromatic hydrocarbons after the strain is cultured for 30 days under following conditions: temperature: 35 DEG C; pH value: 7.3; oil concentration: 0.6%; salt concentration: 0.5%; inoculation amount: 10%; nitrogen source: (NH4)2SO4; and phosphor source: Na2HPO4. The strain (G-40) can adapt with wide ranges of temperature, pH value, and salinity, and has a good prospect and economic value in fields such as petroleum pollutant elimination, biological restoration of petroleum aromatic hydrocarbon polluted soil and water, and the like.

Description

technical field [0001] The invention relates to a petroleum aromatic hydrocarbon degrading bacterium and its application, and belongs to the technical field of microbial degradation of petroleum pollutants. Background technique [0002] During the extraction, transportation, storage, refining and use of crude oil, a large amount of crude oil and its derivative products inevitably enter the environment, causing serious pollution to soil and water. The chemical composition of crude oil is complex, including various toxic substances such as alkanes, cycloalkanes, and aromatics. Some of these aromatic compounds have potential or proven carcinogenic, mutagenic, and mutagenic effects, and can pass through the food chain in animals, plants, and The accumulation in the human body poses a huge threat to the ecological environment and human health. [0003] Microbial degradation has been proven to be a very effective oil pollution removal technology, which has the advantages of cost-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01C02F101/32
CPCB09C1/10C02F3/34C02F2101/322C12N1/205C12R2001/01
Inventor 马立安詹亚斌余维初陶兴玲何山文宋淑芬幸晶晶
Owner YANGTZE UNIVERSITY
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