Degrading bacteria capable of efficiently degrading pesticide chlorothalonil, and application thereof

A high-efficiency degrading bacteria and chlorothalonil technology, which is applied in the field of degrading bacteria, can solve the problem of reducing the degradation rate of pharmaceuticals, and achieve the effect of convenient cultivation and high-efficiency degradation ability
CN103160447AInactive Publication Date: 2013-06-19BEIJING UNIV OF AGRI

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BEIJING UNIV OF AGRI
Publication Date
2013-06-19
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to degrading bacteria capable of efficiently degrading pesticide chlorothalonil, and belongs to the field of biotechnology. A classification name of the degrading bacteria is Enterobacter cloacae B9; and a culture preservation number is CGMCCNo. 5527. Chlorothalonil is an organic chlorine farm-oriented broad spectrum bactericide and is proved to have very strong effects of carcinogenicity, teratogenicity and mutagenicity. Micro-biological degradation gains more and more significance because of rich bacteria resources in the environment without secondary pollution caused by introducing other chemical reagents. Microbe BJQ2 capable of degrading the chlorothalonil can be obtained by directional culture. The Microbe BJQ2 is identified as the Enterobacter cloacae B9 by morphology, physiological and biochemical analysis and 16SrDNA sequence homology alignment. The bacteria are the degrading bacteria by using the chlorothalonil as a sole carbon source. A degrading rate is 79.2% after being cultured for 6 days in an inorganic salt culture liquid; and a degrading rate is 92.3% after being used in a soil for 7 days. The degrading bacteria can be used for degradation of the bactericide chlorothalonil, and biological purification of water bodies, soil or agricultural products which are polluted by the chlorothalonil.
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Description

technical field

[0001] The invention belongs to the field of biological technology, and relates to a degrading bacterium capable of efficiently degrading the fungicide chlorothalonil and its application technical background

[0002] Chlorothalonil (chlorothalonil) is an organochlorine agricultural broad-spectrum fungicide, which has a good control effect on downy mildew, powdery mildew, gray mold and other diseases, and is widely used in vegetables, fruit trees, beans, rice, crops such as wheat. In my country, the annual output exceeds 8,000 tons, and it is the second largest category of widely used agricultural fungicides.

[0003] While it is widely used, it also brings corresponding toxic effects. Studies have shown that chlorothalonil can cause chronic toxicity problems. For example, chlorothalonil can induce sister chromatid exchange in mouse bone marrow lymphocytes and increase the micronucleus rate of bone marrow chromophilic erythrocytes. It is a chemical that muta...

Claims

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