32 results about "Enterobacter cloacae" patented technology

The invention discloses enterobacter mori capable of resisting fish aeromonas and application of the enterobacter mori, the bacterial strain is classified and named as enterobacter mori SCH0241, the enterobacter mori SCH0241 is preserved in the China Center for Type Culture Collection on October 17, 2024, and the preservation number is CCTCC NO: M 20242236. The enterobacter mori SCH0241 disclosed by the invention is derived from the intestinal tract of high-stress-resistance healthy hybrid crucian carassius auratus No. 2, has good contact dependence antibacterial activity on aeromonas veronii, and can improve the capability of resisting aeromonas veronii infection of zebra fish. Through construction of an insertion mutation library and phenotype screening, it is found that a gene cluster of SCH0241, which exerts contact inhibition toxicity, is T6SS-2. The enterobacter mori SCH0241 disclosed by the invention provides a new powerful tool for biological prevention and treatment of fish diseases caused by aeromonas veronii.

The invention discloses a lactobacillus acidophilus FMM01 with the effects of improving the contractility of pelvic floor muscles and repairing mucous membranes and application of the lactobacillus acidophilus FMM01, the lactobacillus acidophilus FMM01 is preserved in the China Center for Type Culture Collection, the preservation date is January 9, 2024, and the preservation number is CCTCC NO: M2024052. The lactobacillus acidophilus FMM01 has the advantages of self-adaption, high acid resistance, strong mucous membrane adhesiveness and no irritation to vaginal mucosa. The lyophilized powder is high in activity and excellent in stability; experimental results show that the lactobacillus acidophilus FMM01 can effectively inhibit bacteria, and especially can effectively inhibit enterobacter cloacae, vibrio parahaemolyticus and / or candida albicans. Besides, the lactobacillus acidophilus FMM01 can improve vaginal relaxation, improve pelvic floor muscle contractility and / or recover vaginal mucosa functions, can promote recovery of healthy florae after mixed vaginitis antibiotic / hormone treatment, improves the treatment effect of mixed vaginitis, and remarkably reduces the recurrence rate of mixed vaginitis.

An antimicrobial peptide having an amino acid sequence selected from SEQ ID NOs: 2-12 is provided. The antimicrobial peptide has antimicrobial activity against Gram-negative bacteria, Campylobacter jejuni, Escherichia coli, Salmonella enterica subsp. enterica serovar typhimurium, Salmonella enterica subsp. enterica serovar choleraesuis, Klebsiella pneumonica, and / or Enterobacter cloacae. A composition, pharmaceutical composition, food additive, cosmetic composition, or hygine product having an antimicrobial peptide including an amino acid sequence selected from SEQ ID NOs: 2-12 is also provided. A method of treating an infectious disease caused by bacteria including a step of administering a pharmaceutical composition having the antimicrobial peptide as an active ingredient is also provided.

The invention relates to the technical field of fertilizers, and particularly discloses a microbial organic fertilizer for promoting growth and development of peas and a preparation method and application of the microbial organic fertilizer. The organic fertilizer liquid is prepared from potassium humate, mineral source potassium fulvate, seaweed essence and compound amino acid; the mixed bacterial liquid is prepared by mixing bacillus amyloliquefaciens FB-9 and enterobacter hormaechei Rs-189. The microbial organic fertilizer provided by the invention can promote growth and nutrient absorption of peas.

The invention discloses an antibacterial 10 peptide, which is characterized in that the sequence of the antibacterial 10 peptide is w-k-r-w-r-r-r-w-w-r-r (dTrp-dLys-dArg-dTrp-dArg-dArg-dTrp-dTrp-dArg-dArg), and all amino acids are D-type amino acids. The antibacterial peptide provided by the invention has good antibacterial activity on various pathogenic bacteria, can be synthesized through an Fmoc solid-phase chemical method, and is low in synthesis difficulty and relatively good in-vivo antibacterial activity. The antibacterial peptide has broad-spectrum killing activity on multidrug-resistant acinetobacter baumannii, carbapenem-resistant pseudomonas aeruginosa and standard strains of acinetobacter baumannii, pseudomonas aeruginosa, klebsiella pneumoniae, escherichia coli, enterobacter cloacae, methicillin-resistant staphylococcus aureus and staphylococcus aureus, and is low in hemolytic toxicity and high in killing activity. Under medium and low concentration (2 microgram / mL to 32 microgram / mL), the cell survival rate can reach more than half, and the cell culture medium has no toxic effect on cells, and is good in stability, strong in operability and low in cost.

The present application relates to the field of microbial technology, and particularly relates to a ginger wilt disease biocontrol strain caused by Enterobacter cloacae and a ginger wilt disease prevention and treatment method. Bacillus cereus The ginger wilt disease biocontrol strain is named Bacillus cereus Y51, which is preserved in the China General Microbiological Culture Collection Center on May 9, 2024, and the preservation number is CGMCC NO.30536. Experiments prove that the Bacillus cereus Y51 exhibits good prevention effect on the Enterobacter cloacae on the ginger plant, and therefore the Bacillus cereus Y51 can be used for preventing and treating the ginger wilt disease, and has the advantages of environmental friendliness, no agricultural residues and green environmental protection. Bacillus cereus The biocontrol strain of the present application fills the blank of the existing ginger wilt disease biological control bacteria in the aspect of targeting specific pathogenic bacteria species.

The application discloses an Enterobacter cloacae and application thereof in tobacco growth promotion, and belongs to the technical field of microorganisms. The Enterobacter cloacae FBH1 is preserved in the China Center for Type Culture Collection (CCTCC), the preservation address is Wuhan University, Wuhan, China, the preservation number is CCTCC NO: M 20241838, and the preservation time is August 23, 2024. The Enterobacter cloacae (FBH1) is isolated for the first time. Enterobacter cloacae The strain FBH1 has good abilities of phosphorus solubilization, potassium solubilization, IAA secretion, iron carrier production and ACC deaminase production, can significantly improve the seed germination rate, germination potential, seedling length and root length, thereby promoting seed germination, can significantly promote plant growth, can reduce the enrichment of phosphorus and potassium elements in tobacco planting layer soil, and can improve the fertilizer utilization efficiency, and therefore has good application prospect.

The invention belongs to the technical field of lactobacillus paracasei, and particularly relates to a lactobacillus paracasei LPa-6 strain and application thereof in atopic dermatitis. The lactobacillus paracasei LPa-6 strain not only has the performance of targeted inhibition of Enterobacter cloacae, Streptococcus sobrinus and Aloscardovia omnicola, but also can be used for increasing the concentration of anti-inflammatory factors, regulating Th1 / Th2 imbalance, increasing the abundance of effective microbial communities in intestinal tracts, and effectively relieving and improving the symptoms of atopic dermatitis, and can be used for preventing and treating enterobacter cloacae, Streptococcus sobrinus and Aloscardovia omnicola, and preventing and treating enterobacter cloacae, Streptococcus sobrinus and Aloscardovia omnicola.

The application discloses a strain of Pseudomonas extremorientalis LJ-6 and application of the strain in preventing and controlling mulberry diseases. The Pseudomonas extremorientalis in the application is preserved in the Guangdong Microbial Culture Collection Center on June 18, 2024, and the preservation number is GDMCC NO: 64766. The Pseudomonas extremorientalis LJ-6 has good antagonistic effects on bacterial pathogenic bacteria such as Caulobacter segnis, Pantoea ananatis, Klebsiella michiganensis and Enterobacter cloacae, and fungal pathogenic bacteria such as Fusarium oxysporum, Curvularia lunata and Guignardia bidwellii. The Pseudomonas extremorientalis LJ-6 has good prevention and control effects on plant diseases caused by the above pathogenic bacteria invading plants, such as mulberry bacterial wilt, mulberry bacterial blight, mulberry leaf spot and mulberry anthracnose. Therefore, the Pseudomonas extremorientalis LJ-6 has good application prospect and value in preventing and controlling plant diseases such as mulberry and preparing disease prevention and control preparation products.

The present application discloses a strain of Enterobacter mori against Aeromonas veronii and its application, which is classified and named as Enterobacter mori (SCH0241). Enterobacter mori SCH0241, preserved in China Center for Type Culture Collection on October 17, 2024, with a preservation number of CCTCC NO: M 20242236. The Enterobacter mori SCH0241 of the present application is derived from the intestine of a highly stress-resistant healthy hybrid crucian carp “Hefang crucian carp No. 2”, has good contact-dependent bacteriostatic activity against Aeromonas veronii, and can improve the ability of zebrafish to resist Aeromonas veronii infection. Through the construction of an insertion mutation library and phenotype screening, it is found that the gene cluster of T6SS-2 of SCH0241 plays a contact inhibition toxicity. The Enterobacter mori SCH0241 of the present application provides a new powerful tool for the biological control of fish diseases caused by Aeromonas veronii.

This invention discloses a strain of *Enterobacter cloacae* EC11 and its applications, belonging to the field of microbial technology. The strain is classified as *Enterobacter cloacae* EC11, with the accession number CCTCC NO:M 2025610. This strain exhibits a hydrolysis zone HC value of 3 on tributyrate agar plates and a lipase activity of 145.28 U / mL. This invention also provides the application of this strain and its produced lipase in the catalytic hydrolysis of tocopherol succinate to prepare tocopherol. The crude enzyme solution obtained by fermenting the lipase strain EC11 was added to a reaction system containing tocopherol succinate for hydrolysis. After 9 hours of reaction, the tocopherol content was increased by 725% compared to the control group. The strain and method provided by this invention have high catalytic efficiency, mild reaction conditions, and are environmentally friendly, making them suitable for the green industrial production of tocopherol.

The invention discloses an application of an HEK-293T cell as a norovirus in-vitro culture host cell, the HEK-293T cell is used as a host bacterium for norovirus in-vitro culture for the first time, and meanwhile, intestinal bacteria such as enterobacter cloacae are introduced as an auxiliary factor, so that the norovirus in-vitro culture efficiency is effectively improved; and a stable and efficient human norovirus in-vitro culture system is successfully established. The defects of low norovirus culture efficiency and poor stability in the prior art are overcome, and stable replication and efficient harvesting of the norovirus are realized.

The invention relates to a complex microbial inoculant for preventing and treating tobacco bacterial wilt and application of the complex microbial inoculant. Strain in the complex microbial inoculant comprises a mulberry enterobacter 65D6 strain, a Ralstonia 56D2 strain, an agrobacterium 18A6 strain, an arthrobacter uretogenes 05-507 strain and a bacillus safensis 05-2101 strain. The five specific strains are compounded and combined for use, an excellent tobacco bacterial wilt prevention and treatment effect is achieved, and experimental research shows that the disease occurrence of tobacco plants treated by the complex microbial inoculant is obviously slowed down, and the disease index is obviously lower than that of a clear water control group and that of a thiediazole copper and chloriure.copper sulfate chemical agent control group. Meanwhile, the five strains can be mutually matched and promoted, a synergistic effect is achieved in the aspect of preventing and treating tobacco bacterial wilt, the WY11 strain is further added for combined use, the effect of preventing and treating the tobacco bacterial wilt is improved, and the better effect of promoting the growth of tobacco plants can be achieved.

Certain embodiments of the invention pertain to a method of treating an infection in a subject caused by an infectious agent other than Escherichia coli, the method comprising administering to the subject arsinothricin or a salt thereof. The infectious agent other than E. coli can be a bacterium, protozoan, helminth, archaebacterium, or a fungus. In preferred embodiments, the infectious agent is Mycobacterium tuberculosis, Mycobacterium bovis, or Enterobacter cloacae. The invention also pertains to a method of treating an infection in a subject caused by an infectious agent, comprising administering to the subject arsinothricin or a salt thereof in combination with an inhibitor of phosphinothricin N-acetyltransferase or arsinothricin N-acetyltransferase. In certain such embodiments, the infectious agent expresses phosphinothricin N-acetyltransferase or arsinothricin N-acetyltransferase. Further embodiments provide compositions comprising arsinothricin or a salt thereof and an inhibitor of phosphinothricin N-acetyltransferase or arsinothricin N-acetyltransferase.

The application relates to the field of environment, resource utilization and agricultural biotechnology, and particularly relates to application of a phosphorus-dissolving bacterial agent for repairing cadmium-polluted soil and simultaneously increasing the content of available phosphorus in the soil, wherein the phosphorus-dissolving bacterial agent comprises phosphorus-dissolving bacteria, bone charcoal, a culture medium and an organic matrix; the phosphorus-dissolving bacteria are enterobacter cloacae HS-6; the phosphorus-dissolving bacterial agent is applied to Cd-polluted soil, and the bioavailability of Cd in the soil is reduced and the content of available phosphorus in the soil is increased through the cooperation of the enterobacter cloacae HS-6 and the bone charcoal. The application can effectively promote the transformation of insoluble phosphate into soluble phosphate, so that the content of available phosphorus in the soil is increased by 96.30%; on the other hand, the application can fix the heavy metal Cd in the soil, so that the content of available Cd in the soil is reduced by about 54.41%, has excellent Cd-fixing capacity, and can relieve the Cd stress of crops, and has good application value in the repair of farmland Cd pollution, the improvement of soil quality and environmental protection.

Six kinds of mixed microorganisms consisting of Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Burkholderia cepacian, and Enterobacter cloacae, and Candida albicans yeast are inoculated into the cosmetic to be tested for storage, and a sample collected from the cosmetic to be tested during the storage is inoculated into an agar medium to which tetrazolium blue is added and cultured, and the number of viable bacteria corresponding to the total number of colony forming units determined in a short time is reduced by a predetermined amount or more from the theoretical number of bacteria inoculated into the agar medium, and this is used as an evaluation criterion for preservation efficacy.

This invention discloses an antimicrobial 10-peptide, characterized by the sequence: w-k-r-w-r-r-w-w-r-r(dTrp-dLys-dArg-dTrp-dArg-dArg-dTrp-dTrp-dArg-dArg), where all amino acids are D-type amino acids. The antimicrobial peptide provided by this invention exhibits good antimicrobial activity against a variety of pathogenic bacteria. This antimicrobial peptide can be synthesized using the Fmoc solid-phase chemical method, which is easy to synthesize and exhibits good in vivo antimicrobial activity. This antimicrobial peptide exhibits broad-spectrum killing activity against multidrug-resistant Acinetobacter baumannii, carbapenem-resistant Pseudomonas aeruginosa, as well as standard strains of Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Enterobacter cloacae, methicillin-resistant Staphylococcus aureus, and Staphylococcus aureus. It has low hemolytic toxicity, and at low to medium concentrations (2 μg / mL to 32 μg / mL), the cell survival rate can reach more than 50%. It has no toxic effect on cells, good stability, strong operability, and low cost.

The invention discloses application of electrochemical active bacteria sensitive to heavy metal pollutants in detection of water quality biotoxicity, and belongs to the technical field of water quality biotoxicity detection.After the electrochemical active bacteria are exposed to toxic pollutants, the water quality biotoxicity is determined according to an electric signal inhibition rate; the toxic pollutants are heavy metal pollutants; the electrochemical active bacteria are enterobacter aerogenes or enterobacter cloacae and are selected from enterobacter. The electrochemical active bacteria sensitive to the heavy metal pollutants are used, so that the sensitivity of determining the biotoxicity of the heavy metal pollutants is expected to be improved, the application bottleneck of low sensitivity is overcome, and a technical support is provided for biotoxicity monitoring work in China.

The invention provides a non-ribosome polypeptide compound as well as a synthetic gene cluster BNP37 and application thereof, the structural sequence of the polypeptide compound is X0-X1-X2-X3-X4-X5-X6-X7-X8-X9-X10-X11, the polypeptide compound is a linear peptide or a cyclic peptide, and the cyclic peptide is a lactam cyclic peptide formed by connecting any one of X1, X3 and X5 with X11, or a lactam cyclic peptide or a lactone cyclic peptide formed by connecting X2 with X11. The compound disclosed by the invention can cause discharge of important ions in bacterial cells by targeting Gram-negative bacterium outer membrane lipid A and Gram-positive bacterium teichoic acid, so that bacterial death is caused. The compound disclosed by the invention has strong in-vivo and in-vitro antibacterial activity on a plurality of multi-drug-resistant gram-negative pathogenic bacteria such as escherichia coli, klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, enterobacter cloacae and neisseria gonorrhoeae and a plurality of multi-drug-resistant gram-positive pathogenic bacteria such as staphylococcus aureus and enterococcus faecium.

The invention discloses a natural bacteriostatic agent based on a lobular formosan lattuce herb extract and a preparation method and application thereof, and belongs to the technical field of lobular formosan lattuce herb extracts.The natural bacteriostatic agent is prepared from, by weight, 4.2-6 parts of an oil phase and 40-50 parts of a water phase, and the water phase is a lobular formosan lattuce herb extracting solution; the oil phase is prepared from the following components in parts by weight: 3.2 to 4.0 parts of lobular formosan lattuce herb essential oil, 0.5 to 1.0 part of sodium alginate and 0.5 to 1.0 part of beta-cyclodextrin. Through the mode, the natural bacteriostatic agent can be used for preparing products for inhibiting the activity of gram-positive bacteria and gram-negative bacteria, and growth and reproduction of methicillin-resistant staphylococcus aureus and ESBL < + >-producing enterobacter cloacae can be well inhibited.

The invention discloses enterobacter and application thereof in prevention and treatment of meloidogyne incognita. The enterobacter is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.35053. The strain has a strong biocontrol effect on meloidogyne incognita, also has a strong growth promoting effect on tomato plant growth, and can make tomatoes blossom and bear fruits in advance.

The present application relates to the field of biological control, in particular to a kind of rape sclerotinia rot prevention and control agent and preparation method thereof.The rape sclerotinia rot prevention and control agent provided by the present application includes enterobacter cloacae fermentation liquor, methylotrophic bacteria fermentation liquor, trichoderma viride fermentation liquor and leptosporella gigantea fermentation liquor.The rape sclerotinia rot prevention and control agent is sprayed on the surface of rape at seedling stage, which can effectively prevent and control rape sclerotinia rot, and the field control efficiency reaches 79%;And enterobacter cloacae, methylotrophic bacteria, trichoderma viride and leptosporella gigantea have obvious synergistic effect in the prevention and control of rape sclerotinia rot.The present application provides a new combined microbial agent for preventing and controlling rape sclerotinia rot, which has wide application prospect due to wide source and remarkable effect.

The invention is applicable to the technical field of microorganisms, and provides an enterobacter cloacae strain and application thereof, the strain is named as enterobacter cloacae DJ, and is preserved in the China Center for Type Culture Collection on July 2, 2025, and the preservation number is CCTCC NO: M20251510. The enterobacter cloacae DJ is separated from rhizosphere soil of Leymus chinensis Tzvel in the western grassland region of Jilin Province, the enterobacter cloacae DJ has a growth promoting effect under the saline-alkaline condition, the germination rate of crop seeds can be obviously increased after the enterobacter cloacae DJ is treated with a bacterial liquid under the saline-alkaline condition, the plant resistance under the saline-alkaline condition can be improved, the utilization rate of saline-alkaline land can be increased, and the application prospect is wide. The method has a wide application prospect in agricultural production.

The invention discloses lactobacillus crispatus GG77 capable of repairing mucosal lesion and preventing pathogenic bacterium infection and application of the lactobacillus crispatus GG77, the lactobacillus crispatus GG77 is preserved in the China Center for Type Culture Collection, the preservation date is November 21, 2025, and the preservation number is CCTCC NO: M 20252646. Compared with the prior art, the invention provides a brand-new lactobacillus crispatus GG77 which mainly has the effects of repairing mucosal lesion and preventing pathogenic bacterium infection. The lactobacillus crispatus GG77 has high acid resistance, can effectively inhibit bacteria, and particularly can effectively inhibit campylobacter Korotkoff, streptococcus agalactiae and / or enterobacter cloacae. Experimental results show that the lactobacillus crispatus GG77 can repair mucosal lesion, prevent vaginitis recurrence, recover genital tract flora and promote calcium ion transport and absorption. Particularly, when the lactobacillus crispatus GG77 and the Witzia clostridium CN365 are combined for use, the lactobacillus crispatus GG77 and the Witzia clostridium CN365 have a synergistic effect, and the effect of transferring and absorbing calcium ions can be remarkably improved.

The invention discloses an intestinal escherichia coli gene detection kit based on a silicon-based micro-fluidic chip and a detection method of the intestinal escherichia coli gene detection kit. The kit provided by the invention comprises a rapid hot start DNA polymerase, a PCR reaction mixed solution, a positive reference substance and a negative reference substance, the kit also comprises the following primers which take the intestinal escherichia coli fliC gene as a target gene, are designed by a PCR fluorescent probe method based on a micro-fluidic chip technical platform: an upstream primer F, a downstream primer R and a detection probe P, and the nucleotide sequences of the primers are shown as SEQ ID NO.1-3. According to the invention, the intestinal escherichia coli gene detection kit which is more comprehensive in detection effect, high in specificity, good in sensitivity, low in omission ratio, convenient, simple and easy to operate is provided.

This invention relates to a peptide and its application in the preparation of antibacterial drugs, belonging to the field of peptide technology. The amino acid sequence of the staple peptide of this invention is G(S5)IAG(S5)IIKIKK-NH2. This includes two S5 residues cyclized to form the staple peptide. The staple peptide of this invention exhibits bioactivity against various carbapenem-resistant Gram-negative bacteria, such as *Citrobacter freundii*, *Pseudomonas aeruginosa*, *Klebsiella pneumoniae*, *Enterobacter cloacae*, *Klebsiella pneumoniae*, and *Escherichia coli*. The staple peptide sp3 kills bacteria by inducing bacterial cell membrane potential dissipation and triggering reactive oxygen species accumulation. Furthermore, sp3 exhibits good stability in trypsin, is not easily degraded, and possesses low cytotoxicity. In summary, the staple peptide sp3 has promising applications and potential in the pharmaceutical field and can serve as a candidate molecule for novel antibacterial drugs.

The invention belongs to the technical field of aquaculture, and particularly relates to identification of an ammonia nitrogen and nitrite degrading bacterium and application of the ammonia nitrogen and nitrite degrading bacterium in sewage denitrification. According to the present invention, the enterobacter cloacae DNFS250501 strain is separated from the largemouth bass pond bottom mud, and the strain has the heterotrophic nitrification-aerobic denitrification capability, can efficiently degrade ammonia nitrogen and nitrite in the water body under the aerobic condition, and has strong adaptability to the substrate concentration, the carbon source type and the carbon nitrogen ratio; and under proper conditions, high-efficiency removal of medium and low-concentration ammonia nitrogen and nitrite can be realized within 24 hours. In addition, the strain does not generate a hemolysis phenomenon, has no pathogenicity to fishes, and is good in biological safety. Therefore, the strain can be used for preparing a denitrification microecological preparation for the aquaculture water body, and can effectively reduce the content of toxic nitrogen-containing substances in the aquaculture water body, improve the water quality environment and reduce the use of chemical agents.

The application discloses a kind of saline-alkali resistant composite microbial inoculant and application thereof, and is specifically related to the field of microbial soil management.The microbial inoculant includes klebsiella, enterobacter cloacae and humic acid, the ratio of each component is klebsiella:enterobacter cloacae:humic acid=1:0.5~2:1~3, wherein klebsiella and enterobacter cloacae are obtained by fermentation culture.The present application uses a specific pH gradient combined with corresponding increasing sodium chloride concentration subsection fermentation process for klebsiella and enterobacter cloacae, effectively carries out directional domestication to strain, so that it can maintain activity and function in extreme environment of high salt and high alkali.

The invention provides a salt-alkali-tolerant phosphate-solubilizing growth-promoting enterobacter cloacae, which is named as enterobacter cloacae BMIP3, is preserved in the China General Microbiological Culture Collection Center (CGMCC), has the preservation number of CGMCC No.36487, and is preserved on November 03, 2025. The salt-alkali-tolerant phosphate-solubilizing growth-promoting enterobacter cloacae has the advantages that the salt-alkali-tolerant phosphate-solubilizing growth-promoting enterobacter cloacae can be applied to salt-alkali-tolerant phosphate-solubilizing growth-promoting enterobacter cloacae The strain has good saline-alkaline tolerance, phosphate solubilizing and growth promoting effects, is stable in growth and high in phosphate solubilizing efficiency in a high saline-alkaline environment, can effectively activate insoluble phosphorus in soil, improves a rhizosphere environment, and remarkably promotes growth of crops such as cotton and the like in salinized soil.

This invention discloses a strain of *Enterobacter cloacae* STR5 and its applications, belonging to the field of microbial technology. The strain is classified as *Enterobacter cloacae* STR5, with accession number CCTCC NO: M 2025607. This strain exhibits a hydrolysis zone HC value of 3.95 on tributyrate plates and a lipase activity of 121.53 U / mL. This invention also provides the application of this strain and its produced lipase in the catalytic hydrolysis of tocopherol succinate to prepare tocopherol. The crude enzyme solution obtained by fermenting strain STR5 was added to a reaction system containing tocopherol succinate for hydrolysis. After 9 hours of reaction, the tocopherol content was increased by 700% compared to the control group. The strain and method provided by this invention have high catalytic efficiency, mild reaction conditions, and are environmentally friendly, making them suitable for the green industrial production of tocopherol.