50 results about "Enterobacter cloacae" patented technology

The invention discloses a urinary system infection pathogen and drug resistance gene detection system and a kit. The detection system comprises forward and reverse primers which are respectively used for detecting escherichia coli, staphylococcus aureus, acinetobacter baumannii, klebsiella pneumoniae, enterococcus faecium, candida glabrata, proteus mirabilis, enterococcus faecalis, candida tropicalis, enterobacter cloacae, candida albicans, pseudomonas aeruginosa and streptococcus agalactiae; the forward primer and the reverse primer are respectively used for detecting the blaCTX-M gene of the extended spectrum beta-lactamase, the forward primer and the reverse primer are used for detecting the blaSHV gene of the extended spectrum beta-lactamase, and the forward primer and the reverse primer are used for detecting the blaTEM gene of the extended spectrum beta-lactamase. According to the forward and reverse primers for detecting the blaNDM gene of the extended-spectrum beta-lactamase, a detection sample is urine. According to the invention, common gram-negative bacteria, gram-positive bacteria and fungi can be detected at the same time, and the problem of missed detection of mixed infection in traditional culture is solved.

The invention discloses enterobacter mori capable of resisting fish aeromonas and application of the enterobacter mori, the bacterial strain is classified and named as enterobacter mori SCH0241, the enterobacter mori SCH0241 is preserved in the China Center for Type Culture Collection on October 17, 2024, and the preservation number is CCTCC NO: M 20242236. The enterobacter mori SCH0241 disclosed by the invention is derived from the intestinal tract of high-stress-resistance healthy hybrid crucian carassius auratus No. 2, has good contact dependence antibacterial activity on aeromonas veronii, and can improve the capability of resisting aeromonas veronii infection of zebra fish. Through construction of an insertion mutation library and phenotype screening, it is found that a gene cluster of SCH0241, which exerts contact inhibition toxicity, is T6SS-2. The enterobacter mori SCH0241 disclosed by the invention provides a new powerful tool for biological prevention and treatment of fish diseases caused by aeromonas veronii.

The invention relates to the technical field of microorganisms, in particular to a ginger bacterial wilt biocontrol strain caused by enterobacter cloacae and a ginger bacterial wilt control method. The name of the ginger bacterial wilt biocontrol strain is bacillus cereus Y51, the strain is preserved in the China General Microbiological Culture Collection Center on May 9, 2024, and the preservation number of the strain is CGMCC NO.30536. The invention further discloses a preparation method of the ginger bacterial wilt biocontrol strain. Experiments prove that the bacillus cereus Y51 has a good prevention effect on the enterobacter cloacae on ginger plants, so that the bacillus cereus Y51 can be used for preventing and treating the ginger blast, and has the advantages of being environmentally friendly, free of pesticide residues and green and environmentally friendly. The biocontrol strain provided by the invention fills up the blank of the existing ginger bacterial wilt biological control strain in the aspect of targeting specific pathogenic bacteria species.

The invention provides a red light carbon quantum dot as well as a preparation method and application thereof. The red light carbon quantum dot is prepared from fulvic acid and polyamine as raw materials through a hydrothermal reaction. The fulvic acid is used as a carbon source, the polyamine is used as a nitrogen source, the carbon quantum dots are prepared through a hydrothermal reaction, the obtained carbon quantum dots are small in size, and the particle size is 1-3 nm; the red light emission wavelengths are 680 nm and 720 nm, and the photoluminescence characteristic is irrelevant to the excitation wavelength; the prepared quantum dot has good biocompatibility, the quantum efficiency is greater than or equal to 20%, the prepared quantum dot has good bacteriostatic and antibacterial activity, and the inhibition rate on staphylococcus aureus, pseudomonas aeruginosa, enterobacter cloacae, escherichia coli, klebsiella pneumoniae or methicillin-resistant staphylococcus aureus under the concentration of 2 mu g / mL can reach 99.9%. Wide application prospects are realized in the aspects of antisepsis, anti-inflammation and wound healing promotion.

The present invention relates to the pharmaceutical field, and provides a boric acid β-lactamase inhibitor or a use thereof. The boric acid β-lactamase inhibitor is a compound shown in formula (I), or an optical isomer or a pharmaceutically acceptable salt thereof: formula (I). The ultra-broad-spectrum boric acid β-lactamase inhibitor of the present invention has an inhibition effect on A, B, C, and D enzymes, and carbapenem drug-resistant bacteria such as Klebsiella pneumoniae, Escherichia coli, Enterobacter cloacae, Acinetobacter baumannii, and Pseudomonas aeruginosa.

Certain embodiments of the invention pertain to a method of treating an infection in a subject caused by an infectious agent other than Escherichia coli, the method comprising administering to the subject arsinothricin or a salt thereof. The infectious agent other than E. coli can be a bacterium, protozoan, helminth, archaebacterium, or a fungus. In preferred embodiments, the infectious agent is Mycobacterium tuberculosis, Mycobacterium bovis, or Enterobacter cloacae. The invention also pertains to a method of treating an infection in a subject caused by an infectious agent, comprising administering to the subject arsinothricin or a salt thereof in combination with an inhibitor of phosphinothricin N-acetyltransferase or arsinothricin N-acetyltransferase. In certain such embodiments, the infectious agent expresses phosphinothricin N-acetyltransferase or arsinothricin N-acetyltransferase. Further embodiments provide compositions comprising arsinothricin or a salt thereof and an inhibitor of phosphinothricin N-acetyltransferase or arsinothricin N-acetyltransferase.

The invention discloses a lactobacillus acidophilus FMM01 with the effects of improving the contractility of pelvic floor muscles and repairing mucous membranes and application of the lactobacillus acidophilus FMM01, the lactobacillus acidophilus FMM01 is preserved in the China Center for Type Culture Collection, the preservation date is January 9, 2024, and the preservation number is CCTCC NO: M2024052. The lactobacillus acidophilus FMM01 has the advantages of self-adaption, high acid resistance, strong mucous membrane adhesiveness and no irritation to vaginal mucosa. The lyophilized powder is high in activity and excellent in stability; experimental results show that the lactobacillus acidophilus FMM01 can effectively inhibit bacteria, and especially can effectively inhibit enterobacter cloacae, vibrio parahaemolyticus and / or candida albicans. Besides, the lactobacillus acidophilus FMM01 can improve vaginal relaxation, improve pelvic floor muscle contractility and / or recover vaginal mucosa functions, can promote recovery of healthy florae after mixed vaginitis antibiotic / hormone treatment, improves the treatment effect of mixed vaginitis, and remarkably reduces the recurrence rate of mixed vaginitis.

The invention belongs to the technical field of microorganisms, and particularly relates to a bacteriophage composition as well as application and a pharmaceutical composition thereof. The bacteriophage composition comprises the following bacteriophages: phi EBU8, phi ECL22 and phi EBU30, the key importance of early treatment in the aspect of treating enterobacter cloacae bacteremia by using the bacteriophage cocktail is proposed, and prophylactic administration possibly provides an opportunity for delayed treatment and rescue of bacteremia.

Certain embodiments of the invention pertain to a method of treating an infection in a subject caused by an infectious agent other than Escherichia coli, the method comprising administering to the subject arsinothricin or a salt thereof. The infectious agent other than E. coli can be a bacterium, protozoan, helminth, archaebacterium, or a fungus. In preferred embodiments, the infectious agent is Mycobacterium tuberculosis, Mycobacterium bovis, or Enterobacter cloacae. The invention also pertains to a method of treating an infection in a subject caused by an infectious agent, comprising administering to the subject arsinothricin or a salt thereof in combination with an inhibitor of phosphinothricin N-acetyltransferase or arsinothricin N-acetyltransferase. In certain such embodiments, the infectious agent expresses phosphinothricin N-acetyltransferase or arsinothricin N-acetyltransferase. Further embodiments provide compositions comprising arsinothricin or a salt thereof and an inhibitor of phosphinothricin N-acetyltransferase or arsinothricin N-acetyltransferase.

The invention discloses an algicidal bacterium TL3 with efficient algicidal activity and application thereof, the algicidal bacterium TL3 is named as Enterobacter cloacae TL3 and is preserved in Guangdong Microbial Culture Collection Center located at No.100, Xianlie Middle Road, Guangzhou City, Guangdong Province, the preservation number is GDMCC No: 65777, and the preservation date is January 13, 2025; the invention also provides an algicidal bacterial agent containing the algicidal bacteria TL3 for degrading or inhibiting water-blooming cyanobacteria. The algicidal bacterium TL3 is obtained by screening and separating in the algicidal period by taking the algicidal habitat of the Lake Taihu as a strain resource library of the efficient algicidal bacterium, has an efficient inhibition effect on microcystis aeruginosa, has a certain algicidal effect on chrysosporium oospermum, cuscuta larakii and anabaena pseudosciaena, is used for various types of cyanobacterial bloom water bodies, and has a good algicidal effect on the water bodies. The application prospect is wide.

An antimicrobial peptide having an amino acid sequence selected from SEQ ID NOs: 2-12 is provided. The antimicrobial peptide has antimicrobial activity against Gram-negative bacteria, Campylobacter jejuni, Escherichia coli, Salmonella enterica subsp. enterica serovar typhimurium, Salmonella enterica subsp. enterica serovar choleraesuis, Klebsiella pneumonica, and / or Enterobacter cloacae. A composition, pharmaceutical composition, food additive, cosmetic composition, or hygine product having an antimicrobial peptide including an amino acid sequence selected from SEQ ID NOs: 2-12 is also provided. A method of treating an infectious disease caused by bacteria including a step of administering a pharmaceutical composition having the antimicrobial peptide as an active ingredient is also provided.

The invention belongs to the technical field of PCR detection, and particularly relates to a composition for detecting pathogenic bacteria, a kit, application and a detection method. The pathogenic bacteria are any one or more of enterococcus faecalis, escherichia coli, klebsiella pneumoniae, enterobacter cloacae, enterococcus faecium and shigella flexneri pathogens, and the composition comprises any one or more of a component A, a component B, a component C, a component D, a component E and a component F; the composition comprises forward and reverse primers and probes aiming at enterococcus faecalis, escherichia coli, klebsiella pneumoniae, enterobacter cloacae, enterococcus faecium and shigella flexneri. The composition provided by the invention can simultaneously detect and distinguish enterococcus faecalis, escherichia coli, klebsiella pneumoniae, enterobacter cloacae, enterococcus faecium and shigella flexneri, has high sensitivity, specificity and precision, is helpful to take treatment measures in time and customize personalized treatment schemes, reduces unnecessary use of antibiotics, and has a good application prospect. The generation of drug resistance is reduced.

The invention relates to the technical field of fertilizers, and particularly discloses a microbial organic fertilizer for promoting growth and development of peas and a preparation method and application of the microbial organic fertilizer. The organic fertilizer liquid is prepared from potassium humate, mineral source potassium fulvate, seaweed essence and compound amino acid; the mixed bacterial liquid is prepared by mixing bacillus amyloliquefaciens FB-9 and enterobacter hormaechei Rs-189. The microbial organic fertilizer provided by the invention can promote growth and nutrient absorption of peas.

The invention discloses an antibacterial 10 peptide, which is characterized in that the sequence of the antibacterial 10 peptide is w-k-r-w-r-r-r-w-w-r-r (dTrp-dLys-dArg-dTrp-dArg-dArg-dTrp-dTrp-dArg-dArg), and all amino acids are D-type amino acids. The antibacterial peptide provided by the invention has good antibacterial activity on various pathogenic bacteria, can be synthesized through an Fmoc solid-phase chemical method, and is low in synthesis difficulty and relatively good in-vivo antibacterial activity. The antibacterial peptide has broad-spectrum killing activity on multidrug-resistant acinetobacter baumannii, carbapenem-resistant pseudomonas aeruginosa and standard strains of acinetobacter baumannii, pseudomonas aeruginosa, klebsiella pneumoniae, escherichia coli, enterobacter cloacae, methicillin-resistant staphylococcus aureus and staphylococcus aureus, and is low in hemolytic toxicity and high in killing activity. Under medium and low concentration (2 microgram / mL to 32 microgram / mL), the cell survival rate can reach more than half, and the cell culture medium has no toxic effect on cells, and is good in stability, strong in operability and low in cost.

The present invention relates to a method for preparing a cosmetic sample, which is characterized in that: six kinds of mixed microorganisms comprising five kinds of bacteria, i.e., Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Burkholderia cepacian, and Enterobacter cloacae, and Candida albicans yeast, are inoculated into a cosmetic sample to be tested, and are stored; a sample collected from the cosmetic to be tested during the storage period is inoculated into an agar culture medium to which tetrazole blue is added and cultured; the number of viable cells corresponding to the total number of colony formation units measured in a short-time culture state is reduced by a predetermined number or more with respect to the theoretical number of cells inoculated in the agar culture medium.

The application provides a preparation method of earthworm antibacterial crude protein and belongs to the technical field of biotechnology. The technical scheme comprises the following steps: stimulating earthworms with tea dregs liquid, homogenizing the earthworms, centrifugal separation, and obtaining earthworm antibacterial crude protein after freeze-drying. The earthworm species that can be used in the application include Octochaetus porrectus, Metaphire cummingi, Drawida japonica, Drawida hupehensis or Allolobophora clavata. The obtained earthworm antibacterial crude protein can inhibit Pseudomonas aeruginosa, Enterobacter cloacae, Proteus vulgaris and Serratia marcescens after being dissolved in sterile water. The application has the advantages of wide raw material sources, simple preparation method and the like, and has potential values in aspects of livestock breeding, medical treatment and health care and the like.

Described herein are compositions, methods, and kits for detecting wound pathogens. One embodiment described herein is primer pairs and probes for individual, simultaneous, or sequential multiplex polymerase chain reaction (PCR) based assays for the detection of Acinetobacter baumannii, Bacteroides fragilis, Clostridium perfringens, Enterobacter cloacae, Enterococcus faecalis, Enterococcus faecium, Escherichia coli, Klebsiella pneumoniae, Klebsiella Oxytoca, Klebsiella aerogenes, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Serratia marcescens, Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus pyogenes, Streptococcus anginosus, Staphylococcus lugdunensis, Providencia stuartii, Stenotrophomonas maltophilia, Morganella morganii, Citrobacter koseri, Citrobacter freundii, Citrobacter braakii, Peptoniphilus asaccharolyticus, Peptoniphilus harei, Peptoniphilus ivorii, Peptostreptococcus anaerobius, Finegoldia magna, Clostridium septicum, Paeniclostridium sordellii (Clostridium sordellii), Clostridium novyi A, B, Clostridium histolyticum, Staphylococcus epidermidis, Staphylococcus haemolyticus, Corynebacterium striatum, Candida albicans, Candida auris, Candida glabrata, Candida krusei, Candida parapsilosis, or Candida tropicalis.

The present application relates to the field of microbial technology, and particularly relates to a ginger wilt disease biocontrol strain caused by Enterobacter cloacae and a ginger wilt disease prevention and treatment method. Bacillus cereus The ginger wilt disease biocontrol strain is named Bacillus cereus Y51, which is preserved in the China General Microbiological Culture Collection Center on May 9, 2024, and the preservation number is CGMCC NO.30536. Experiments prove that the Bacillus cereus Y51 exhibits good prevention effect on the Enterobacter cloacae on the ginger plant, and therefore the Bacillus cereus Y51 can be used for preventing and treating the ginger wilt disease, and has the advantages of environmental friendliness, no agricultural residues and green environmental protection. Bacillus cereus The biocontrol strain of the present application fills the blank of the existing ginger wilt disease biological control bacteria in the aspect of targeting specific pathogenic bacteria species.

The application discloses an Enterobacter cloacae and application thereof in tobacco growth promotion, and belongs to the technical field of microorganisms. The Enterobacter cloacae FBH1 is preserved in the China Center for Type Culture Collection (CCTCC), the preservation address is Wuhan University, Wuhan, China, the preservation number is CCTCC NO: M 20241838, and the preservation time is August 23, 2024. The Enterobacter cloacae (FBH1) is isolated for the first time. Enterobacter cloacae The strain FBH1 has good abilities of phosphorus solubilization, potassium solubilization, IAA secretion, iron carrier production and ACC deaminase production, can significantly improve the seed germination rate, germination potential, seedling length and root length, thereby promoting seed germination, can significantly promote plant growth, can reduce the enrichment of phosphorus and potassium elements in tobacco planting layer soil, and can improve the fertilizer utilization efficiency, and therefore has good application prospect.

The invention belongs to the technical field of lactobacillus paracasei, and particularly relates to a lactobacillus paracasei LPa-6 strain and application thereof in atopic dermatitis. The lactobacillus paracasei LPa-6 strain not only has the performance of targeted inhibition of Enterobacter cloacae, Streptococcus sobrinus and Aloscardovia omnicola, but also can be used for increasing the concentration of anti-inflammatory factors, regulating Th1 / Th2 imbalance, increasing the abundance of effective microbial communities in intestinal tracts, and effectively relieving and improving the symptoms of atopic dermatitis, and can be used for preventing and treating enterobacter cloacae, Streptococcus sobrinus and Aloscardovia omnicola, and preventing and treating enterobacter cloacae, Streptococcus sobrinus and Aloscardovia omnicola.

The application discloses a strain of Pseudomonas extremorientalis LJ-6 and application of the strain in preventing and controlling mulberry diseases. The Pseudomonas extremorientalis in the application is preserved in the Guangdong Microbial Culture Collection Center on June 18, 2024, and the preservation number is GDMCC NO: 64766. The Pseudomonas extremorientalis LJ-6 has good antagonistic effects on bacterial pathogenic bacteria such as Caulobacter segnis, Pantoea ananatis, Klebsiella michiganensis and Enterobacter cloacae, and fungal pathogenic bacteria such as Fusarium oxysporum, Curvularia lunata and Guignardia bidwellii. The Pseudomonas extremorientalis LJ-6 has good prevention and control effects on plant diseases caused by the above pathogenic bacteria invading plants, such as mulberry bacterial wilt, mulberry bacterial blight, mulberry leaf spot and mulberry anthracnose. Therefore, the Pseudomonas extremorientalis LJ-6 has good application prospect and value in preventing and controlling plant diseases such as mulberry and preparing disease prevention and control preparation products.

The present application discloses a strain of Enterobacter mori against Aeromonas veronii and its application, which is classified and named as Enterobacter mori (SCH0241). Enterobacter mori SCH0241, preserved in China Center for Type Culture Collection on October 17, 2024, with a preservation number of CCTCC NO: M 20242236. The Enterobacter mori SCH0241 of the present application is derived from the intestine of a highly stress-resistant healthy hybrid crucian carp “Hefang crucian carp No. 2”, has good contact-dependent bacteriostatic activity against Aeromonas veronii, and can improve the ability of zebrafish to resist Aeromonas veronii infection. Through the construction of an insertion mutation library and phenotype screening, it is found that the gene cluster of T6SS-2 of SCH0241 plays a contact inhibition toxicity. The Enterobacter mori SCH0241 of the present application provides a new powerful tool for the biological control of fish diseases caused by Aeromonas veronii.

This invention discloses a strain of *Enterobacter cloacae* EC11 and its applications, belonging to the field of microbial technology. The strain is classified as *Enterobacter cloacae* EC11, with the accession number CCTCC NO:M 2025610. This strain exhibits a hydrolysis zone HC value of 3 on tributyrate agar plates and a lipase activity of 145.28 U / mL. This invention also provides the application of this strain and its produced lipase in the catalytic hydrolysis of tocopherol succinate to prepare tocopherol. The crude enzyme solution obtained by fermenting the lipase strain EC11 was added to a reaction system containing tocopherol succinate for hydrolysis. After 9 hours of reaction, the tocopherol content was increased by 725% compared to the control group. The strain and method provided by this invention have high catalytic efficiency, mild reaction conditions, and are environmentally friendly, making them suitable for the green industrial production of tocopherol.

The invention discloses an application of an HEK-293T cell as a norovirus in-vitro culture host cell, the HEK-293T cell is used as a host bacterium for norovirus in-vitro culture for the first time, and meanwhile, intestinal bacteria such as enterobacter cloacae are introduced as an auxiliary factor, so that the norovirus in-vitro culture efficiency is effectively improved; and a stable and efficient human norovirus in-vitro culture system is successfully established. The defects of low norovirus culture efficiency and poor stability in the prior art are overcome, and stable replication and efficient harvesting of the norovirus are realized.

The invention relates to a complex microbial inoculant for preventing and treating tobacco bacterial wilt and application of the complex microbial inoculant. Strain in the complex microbial inoculant comprises a mulberry enterobacter 65D6 strain, a Ralstonia 56D2 strain, an agrobacterium 18A6 strain, an arthrobacter uretogenes 05-507 strain and a bacillus safensis 05-2101 strain. The five specific strains are compounded and combined for use, an excellent tobacco bacterial wilt prevention and treatment effect is achieved, and experimental research shows that the disease occurrence of tobacco plants treated by the complex microbial inoculant is obviously slowed down, and the disease index is obviously lower than that of a clear water control group and that of a thiediazole copper and chloriure.copper sulfate chemical agent control group. Meanwhile, the five strains can be mutually matched and promoted, a synergistic effect is achieved in the aspect of preventing and treating tobacco bacterial wilt, the WY11 strain is further added for combined use, the effect of preventing and treating the tobacco bacterial wilt is improved, and the better effect of promoting the growth of tobacco plants can be achieved.

The invention relates to the technical field of microbial fermentation, and discloses a processing method for biosynthesizing chitosan by using bacteria, which comprises the following steps: S1, microbial culture: culturing enterobacter cloacae to a growth state; according to the processing method for biosynthesizing the chitosan by utilizing the bacteria, the chitosan generated by utilizing the enterobacter cloacae has good biodegradability, which means that the chitosan can be naturally decomposed by a human body after being used, so that the burden on the environment is reduced, and compared with other bacteria, the enterobacter cloacae possibly has lower biosynthesis cost, so that the environment is protected. According to the present invention, the enterobacter cloacae can grow in a variety of environments such as soil, water and other biomass resources, such that the rich raw material source is provided for the chitosan production, and the chitosan synthesized by the enterobacter cloacae can be combined with other compounds or agents so as to reduce the production cost of the chitosan; the composite material or medicine with different functions and purposes can be formed and can be used as a food additive, a medicine carrier or a biological material and the like.

Certain embodiments of the invention pertain to a method of treating an infection in a subject caused by an infectious agent other than Escherichia coli, the method comprising administering to the subject arsinothricin or a salt thereof. The infectious agent other than E. coli can be a bacterium, protozoan, helminth, archaebacterium, or a fungus. In preferred embodiments, the infectious agent is Mycobacterium tuberculosis, Mycobacterium bovis, or Enterobacter cloacae. The invention also pertains to a method of treating an infection in a subject caused by an infectious agent, comprising administering to the subject arsinothricin or a salt thereof in combination with an inhibitor of phosphinothricin N-acetyltransferase or arsinothricin N-acetyltransferase. In certain such embodiments, the infectious agent expresses phosphinothricin N-acetyltransferase or arsinothricin N-acetyltransferase. Further embodiments provide compositions comprising arsinothricin or a salt thereof and an inhibitor of phosphinothricin N-acetyltransferase or arsinothricin N-acetyltransferase.

Certain embodiments of the invention pertain to a method of treating an infection in a subject caused by an infectious agent other than Escherichia coli, the method comprising administering to the subject arsinothricin or a salt thereof. The infectious agent other than E. coli can be a bacterium, protozoan, helminth, archaebacterium, or a fungus. In preferred embodiments, the infectious agent is Mycobacterium tuberculosis, Mycobacterium bovis, or Enterobacter cloacae. The invention also pertains to a method of treating an infection in a subject caused by an infectious agent, comprising administering to the subject arsinothricin or a salt thereof in combination with an inhibitor of phosphinothricin N-acetyltransferase or arsinothricin N-acetyltransferase. In certain such embodiments, the infectious agent expresses phosphinothricin N-acetyltransferase or arsinothricin N-acetyltransferase. Further embodiments provide compositions comprising arsinothricin or a salt thereof and an inhibitor of phosphinothricin N-acetyltransferase or arsinothricin N-acetyltransferase.

The application relates to the field of environment, resource utilization and agricultural biotechnology, and particularly relates to application of a phosphorus-dissolving bacterial agent for repairing cadmium-polluted soil and simultaneously increasing the content of available phosphorus in the soil, wherein the phosphorus-dissolving bacterial agent comprises phosphorus-dissolving bacteria, bone charcoal, a culture medium and an organic matrix; the phosphorus-dissolving bacteria are enterobacter cloacae HS-6; the phosphorus-dissolving bacterial agent is applied to Cd-polluted soil, and the bioavailability of Cd in the soil is reduced and the content of available phosphorus in the soil is increased through the cooperation of the enterobacter cloacae HS-6 and the bone charcoal. The application can effectively promote the transformation of insoluble phosphate into soluble phosphate, so that the content of available phosphorus in the soil is increased by 96.30%; on the other hand, the application can fix the heavy metal Cd in the soil, so that the content of available Cd in the soil is reduced by about 54.41%, has excellent Cd-fixing capacity, and can relieve the Cd stress of crops, and has good application value in the repair of farmland Cd pollution, the improvement of soil quality and environmental protection.

Six kinds of mixed microorganisms consisting of Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Burkholderia cepacian, and Enterobacter cloacae, and Candida albicans yeast are inoculated into the cosmetic to be tested for storage, and a sample collected from the cosmetic to be tested during the storage is inoculated into an agar medium to which tetrazolium blue is added and cultured, and the number of viable bacteria corresponding to the total number of colony forming units determined in a short time is reduced by a predetermined amount or more from the theoretical number of bacteria inoculated into the agar medium, and this is used as an evaluation criterion for preservation efficacy.