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Enterobacter cloacae SRPG-70 and application thereof in salt stress relieving and growth promoting

A technology of Enterobacter cloacae and strains, which is applied in the field of agricultural biology, can solve the problems of few reported strains, achieve stable application effects, reduce the use of chemical fertilizers, and relieve salt stress

Inactive Publication Date: 2015-04-29
SHIHEZI UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there are few reports about the unique geographical and climatic characteristics of Xinjiang, and there are no reports about Enterobacter cloacae that can relieve salt stress and promote plant growth

Method used

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  • Enterobacter cloacae SRPG-70 and application thereof in salt stress relieving and growth promoting
  • Enterobacter cloacae SRPG-70 and application thereof in salt stress relieving and growth promoting
  • Enterobacter cloacae SRPG-70 and application thereof in salt stress relieving and growth promoting

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Identification of Physiological and Biochemical Properties

[0018] Physiological and biochemical characteristics and 16S rDNA molecular identification were carried out on the salt-dissolving-promoting bacteria SRPG-70, and the species of the salt-dissolving-promoting bacteria was determined from the molecular level.

[0019] The identification results are shown in Table 1.

[0020] Table 1 Physiological and biochemical characteristics of strain SRPG-70

[0021]

[0022]

[0023] +: Positive; -: Negative

[0024] 16S rDNA identification

[0025] The size of the 16S rDNA sequence fragment of SRPG-70 obtained by sequencing is 1459bp. The sequence homology comparison analysis was carried out on the NCBI website. Combined with the identification results of physiological and biochemical characteristics, the morphological, physiological and biochemical characteristics of the strain SRPG-70 are consistent with Enterobacter cloacae feature. Thus identified,...

Embodiment 2

[0026] Example 2: Determination of bacterial colonization after inoculation of Enterobacter cloacae SRPG-70 bacteria under salt stress conditions

[0027] The colonization of Enterobacter cloacae SRPG-70 was observed and analyzed by scanning electron microscope (SEM) (JSM-670 of Tokyo JEOL Company, Japan). Cotton root samples were treated with a series of ethanol solutions, finally dehydrated with 100% acetone, and again critical point dried with liquid carbon dioxide using the CPD 030 (BAL-TEC, Germany) method. Then the sample was sprayed with gold (sputter coater SCD, 005, BAL-TEC, Germany) and then examined by scanning electron microscope.

[0028] The scanning electron microscope observation results of the samples taken from the base of the rhizome, the middle of the root and the tip of the root showed that the strain SRPG-70 had colony distribution in each root segment, and the root segment of the root of the rhizome had a higher density of bacteria, which were generally ...

Embodiment 3

[0029] Example 3: Effect of inoculating Enterobacter cloacae SRPG-70 on cotton biomass under salt stress conditions

[0030] Treat cotton seeds with bacteria. When the cotton grows to the three-leaf stage in the saline-alkaline soil pot test, measure the length from the growth point to the root tip of all the plants in the same treatment, that is, the plant height, and take the average value, which is the average plant height of the treated plants. high. Weigh the total weight of all the plants of the same treatment, then place them in a drying oven, kill them at 110°C for 10 minutes, then transfer them to 80°C for 24 hours to constant weight, take them out and weigh them. The total weight before drying was divided by the number of weighed plants to obtain the average fresh weight of the treated plants. The total weight after drying was divided by the number of weighed plants to obtain the average dry weight of the treated plants. The growth of potted cotton is shown in Tabl...

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Abstract

The invention discloses enterobacter cloacae SRPG-70 which is screened from Sinkiang salinity soil and has the effect of remarkably relieving salt stress to promote crop growth. Authentications in the aspects of physiological and biochemical characteristics show that the enterobacter cloacae SRPG-70 is a gram negative bacterium. By utilizing the characteristics of the enterobacter cloacae SRPG-70, pot experiment proves that the germination rate in treatment of 0.8% of NaCl is improved by 22.4% relative to sterile treatment; under salinization condition, the dry weight of cotton is improved by 28.8% by the SRPG-70; the plant height is increased by 14.2%; under salinization-free condition, the dry weight of cotton is improved by 14.9% by the SRPG-70; the plant height is improved by 11.5%. Through electron microscopy observation, the SRPG-70 can be successfully colonized at the middle parts of root tips and root base parts of plant roots. The cotton inoculated with the SRPG-70 is capable of adjusting the uptake, accumulation, transportation and distribution of metal ions. The SRPG-70 can be used for replacing chemical fertilizers to provide salt stress relieving and growth promoting effects and has the advantages of being strong in adaptive capacity to environment and stable in application effect; therefore, the SRPG-70 can be used for developing microbial agents and has wide application prospect to solve the soil salinization problem.

Description

technical field [0001] The invention relates to Enterobacter cloacae SRPG-70, which belongs to the field of agricultural biotechnology. More specifically, the present invention relates to a strain of Enterobacter cloacae SRPG-70 capable of dissolving phosphorus, dissolving potassium and nitrogen-fixing and its use for promoting growth by dissolving salt. [0002] The strain has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on June 30, 2014. The preservation number is CGMCC No.9395, and the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing. Promote plant growth instead of chemical fertilizers. More specifically, the strain can increase the germination rate of cotton seeds and increase the biomass growth of cotton seedlings under salt stress conditions. Background technique [0003] The unique arid and semi-arid climate conditions in Northwest China have caused serious sho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/01
CPCA01N63/00C12N1/205C12R2001/01
Inventor 武占省李春何艳慧樊艳爽凃亮
Owner SHIHEZI UNIVERSITY
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