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Bagasse immobilized bacterium and application thereof to sulfamethoxazole pollution remediation of farmland soil

A technology of pollution remediation and bagasse, which is applied to the restoration of contaminated soil on/in organic carriers, and microbial-based methods, etc. It can solve the problems of high residues and refractory degradation of sulfonamide antibiotics, etc., and achieve bacterial density Larger, the effect of promoting soil microbial activity and quantity, and improving efficiency

Inactive Publication Date: 2017-02-22
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The degradation rate of the sulfonamide antibiotics in the pig manure is relatively fast by the methods of the prior art, and once the antibiotics enter the soil, they will first undergo adsorption with the soil, and under certain conditions, some antibiotics will form a stable and inactive bonded residue with the soil organic matter. The sulfa antibiotics in the soil have the characteristics of refractory degradation and high residues, and there are few reports on the remediation of soil sulfa antibiotic pollution.

Method used

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  • Bagasse immobilized bacterium and application thereof to sulfamethoxazole pollution remediation of farmland soil
  • Bagasse immobilized bacterium and application thereof to sulfamethoxazole pollution remediation of farmland soil
  • Bagasse immobilized bacterium and application thereof to sulfamethoxazole pollution remediation of farmland soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Example 1: Comparison of the effects of free bacteria (HM1) and bagasse immobilized bacteria (HM1) on degradation of sulfamethoxazole in soil

[0072] The purified strain HM1 was inoculated into 50mL liquid culture medium, and the pH was adjusted to 7.0. The culture was shaken on a shaker at 28°C and 150r / min for 36h until the biomass of the microorganisms reached the maximum, and 4mL of bacterial solution was taken respectively. Centrifuge in a centrifuge tube at 4000r / min for 10 minutes, then pour out the supernatant in the centrifuge tube, supplement with an appropriate amount of phosphate buffer and continue centrifugation for 10 minutes, repeat this 3 times, and place the bacterial suspension in a refrigerator at 4°C for use.

[0073] The test red soil was taken from the arboretum of South China Agricultural University. Its texture was silty sandy soil, pH value (water / soil=2.5 / 1) was 5.35, organic matter was 7.89g / kg, nitrogen, phosphorus, and potassium were 0.71. 0.72...

Embodiment 2

[0079] Example 2: Effect of sterilized and non-sterilized soil on the degradation of sulfamethoxazole in soil by bagasse immobilized bacteria (HM1)

[0080] The bacterial suspension was the same as in Example 1. The bacteria added in each treatment were all bagasse-immobilized bacteria (HM1). The test soil and bagasse-immobilized bacteria (HM1) were prepared as in Example 1. Before the experiment, the sulfamethoxin in the contaminated soil was determined The initial concentration of azole was 92.35mg / kg.

[0081] This embodiment sets the following 4 processes:

[0082] Treatment 1: Non-sterile soil and bacteria treatment (add bagasse immobilized HM1 (1ml: 3g))

[0083] Treatment 2: Non-sterile soil control treatment (no bacteria added)

[0084] Treatment 3: Sterilized soil and bacteria treatment (add bagasse immobilized HM1 (1ml: 3g))

[0085] Treatment 4: Sterilized soil control treatment (no bacteria added)

[0086] There were 18 beakers under each treatment, and samples were taken on ...

Embodiment 3

[0088] Example 3: Heavy metal ion Cd in soil 2+ And Pb 2 Effect of Immobilized Bacteria on Bagasse (HM1) Degrading Sulfamethoxazole in Soil

[0089] The bacterial suspension was the same as in Example 1. The bacteria added in each treatment were all bagasse-immobilized bacteria (HM1). The test soil and bagasse-immobilized bacteria (HM1) were prepared as in Example 1, but the sterilized inorganic salt liquid medium was used PbC1 in 0.01% 2 And CdC1 2 Before the experiment, the initial concentration of sulfamethoxazole in the contaminated soil was determined to be 92.35mg / kg.

[0090] This embodiment sets the following 4 processes:

[0091] Treatment 1: Control treatment (without bacteria)

[0092] Treatment 2: Add bacteria (add bagasse immobilized HM1 (1ml: 3g))

[0093] Treatment 3: Cd 2+ +Bacteria treatment (Cd 2+ + HM1 immobilized with bagasse (1ml: 3g))

[0094] Processing 4: Pb 2+ +Bacteria treatment (Pb 2+ + HM1 immobilized with bagasse (1ml: 3g))

[0095] There are 18 beakers under...

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Abstract

The invention discloses a bagasse immobilized bacterium and application thereof to sulfamethoxazole pollution remediation of farmland soil. According to the bagasse immobilized bacterium, Enterobacter cloacae is immobilized by adopting bagasse, and when the Enterobacter cloacae is inoculated to a bagasse vector, a bacterial suspension is prepared by culturing in advance, and then the Enterobacter cloacae is immobilized onto the bagasse vector treated by an inorganic salt culture solution to establish an efficient and stable agricultural waste immobilized bacterium system. The bagasse immobilized bacterium is low in cost, and when the soil is remediated, simplicity and environment-friendliness are achieved, and secondary pollution is avoided; in addition, the bioavailability of sulfonamides can be improved, and the microbiological degradation efficiency of the sulfonamides is improved.

Description

[0001] 【Technical Field】 [0002] The invention relates to bagasse immobilized bacteria and its application in remediation of sulfamethoxazole pollution in farmland soil. [0003] 【Background technique】 [0004] Sulfonamide antibiotics are one of the most widely used anti-infective drugs in the animal husbandry industry. They have a broad antibacterial spectrum, stable properties, and low prices. They have a good effect on controlling infectious diseases and are widely used at home and abroad. In recent decades, the demand for sulfa drugs in the international market has been relatively stable. It has always been the main force of antibacterial and anti-inflammatory in the vast developing countries, especially the poorer countries, and the dosage is relatively large. In developed countries, sulfa drugs are mostly added to feed as therapeutic and preventive drugs for livestock and poultry, and their dosage is relatively large and the demand is relatively stable. With the rapid develo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/02B09C1/10C12R1/01
CPCC12N11/02B09C1/10
Inventor 赵月春胡辉敏
Owner SOUTH CHINA AGRI UNIV
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