Acetobacter culture medium and acetic bacteria culture method
A cultivation method and technology of acetic acid bacteria, applied in the cultivation field of acetic acid bacteria culture medium and acetic acid bacteria, can solve problems such as wasting resources and polluting the environment, and achieve growth promotion, fermentation effect improvement, bacterial density and titratable acid content Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0031] In the present invention, the preparation method of described yellow slurry water comprises the steps:
[0032] (1) mixing and grinding the dry soybean after soaking with water to obtain soybean milk;
[0033] (2) decoct the soymilk obtained in step (1) and then lower the temperature, mix the cooled soymilk with a halogenating agent and stand to obtain bean curd, and squeeze the bean curd to obtain yellow syrup.
[0034] In the present invention, the soaking time in step (1) is 7-9 hours; preferably 8 hours.
[0035] In the present invention, the mass-volume ratio of the dry soybean to water is 1-3:7-21g / ml; preferably 1-3:10-18g / ml; more preferably 2:12-16g / ml; more Preferably it is 2:14 g / ml.
[0036] In the present invention, the decoction time in step (2) is 8-12 min; preferably 9-11 min; more preferably 10 min.
[0037] In the present invention, the cooling is to reduce the temperature to 83-87°C; preferably 84-86°C; more preferably 85°C.
[0038] In the presen...
Embodiment 1
[0047] A method for culturing acetic acid bacteria, wherein the acetic acid bacteria are inoculated in the acetic acid bacteria culture medium, and the inoculation ratio of the culture medium is 2 × 10 5 / ml, the incubation time was 16h, and the temperature was 29°C;
[0048] The acetic acid bacteria culture medium includes yellow slurry water and genistein, and the final concentration of the genistein in the yellow slurry water is 30 mg / L;
[0049] The steps of the preparation method of the yellow slurry water are as follows:
[0050] (1) mixing and grinding with water after soaking dry soybean for 7h to obtain soybean milk; the mass volume ratio of described dry soybean and water is 1: 7g / ml;
[0051] (2) the soymilk obtained in step (1) is boiled for 8min and then cooled to 83°C, the soymilk after the cooling is mixed with a halogenating agent and left to stand for 15min to obtain bean curd, and the bean curd is squeezed to obtain yellow syrup; The mass ratio of halogen a...
Embodiment 2
[0053] A method for culturing acetic acid bacteria, inoculating Acetobacter orlani in the acetic acid bacteria medium for cultivation, and the inoculation ratio of the medium is 6×10 5 / ml, the incubation time was 48h, and the temperature was 33°C;
[0054] The acetic acid bacteria culture medium includes yellow syrup water and genistein, and the final concentration of the genistein in the yellow syrup water is 80 mg / L;
[0055] The steps of the preparation method of the yellow slurry water are as follows:
[0056] (1) mixing and grinding with water after soaking dry soybean for 9h to obtain soybean milk; the mass volume ratio of described dry soybean and water is 3: 21g / ml;
[0057] (2) the soymilk obtained in step (1) is boiled for 12min and then cooled to 87°C, the soymilk after the cooling is mixed with a halogenating agent and left to stand for 25min to obtain bean curd, and the bean curd is squeezed to obtain yellow syrup; The mass ratio of halogen agent is 55:1.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com