Acetobacter culture medium and acetic bacteria culture method

A cultivation method and technology of acetic acid bacteria, applied in the cultivation field of acetic acid bacteria culture medium and acetic acid bacteria, can solve problems such as wasting resources and polluting the environment, and achieve growth promotion, fermentation effect improvement, bacterial density and titratable acid content Effect

Pending Publication Date: 2022-07-22
HUAINAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, due to the limitations of technological conditions and processing equipment, a large amount of soybean yellow slurry water is discharged as waste, which not only wastes resources but also pollutes the environment

Method used

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  • Acetobacter culture medium and acetic bacteria culture method
  • Acetobacter culture medium and acetic bacteria culture method
  • Acetobacter culture medium and acetic bacteria culture method

Examples

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preparation example Construction

[0031] In the present invention, the preparation method of described yellow slurry water comprises the steps:

[0032] (1) mixing and grinding the dry soybean after soaking with water to obtain soybean milk;

[0033] (2) decoct the soymilk obtained in step (1) and then lower the temperature, mix the cooled soymilk with a halogenating agent and stand to obtain bean curd, and squeeze the bean curd to obtain yellow syrup.

[0034] In the present invention, the soaking time in step (1) is 7-9 hours; preferably 8 hours.

[0035] In the present invention, the mass-volume ratio of the dry soybean to water is 1-3:7-21g / ml; preferably 1-3:10-18g / ml; more preferably 2:12-16g / ml; more Preferably it is 2:14 g / ml.

[0036] In the present invention, the decoction time in step (2) is 8-12 min; preferably 9-11 min; more preferably 10 min.

[0037] In the present invention, the cooling is to reduce the temperature to 83-87°C; preferably 84-86°C; more preferably 85°C.

[0038] In the presen...

Embodiment 1

[0047] A method for culturing acetic acid bacteria, wherein the acetic acid bacteria are inoculated in the acetic acid bacteria culture medium, and the inoculation ratio of the culture medium is 2 × 10 5 / ml, the incubation time was 16h, and the temperature was 29°C;

[0048] The acetic acid bacteria culture medium includes yellow slurry water and genistein, and the final concentration of the genistein in the yellow slurry water is 30 mg / L;

[0049] The steps of the preparation method of the yellow slurry water are as follows:

[0050] (1) mixing and grinding with water after soaking dry soybean for 7h to obtain soybean milk; the mass volume ratio of described dry soybean and water is 1: 7g / ml;

[0051] (2) the soymilk obtained in step (1) is boiled for 8min and then cooled to 83°C, the soymilk after the cooling is mixed with a halogenating agent and left to stand for 15min to obtain bean curd, and the bean curd is squeezed to obtain yellow syrup; The mass ratio of halogen a...

Embodiment 2

[0053] A method for culturing acetic acid bacteria, inoculating Acetobacter orlani in the acetic acid bacteria medium for cultivation, and the inoculation ratio of the medium is 6×10 5 / ml, the incubation time was 48h, and the temperature was 33°C;

[0054] The acetic acid bacteria culture medium includes yellow syrup water and genistein, and the final concentration of the genistein in the yellow syrup water is 80 mg / L;

[0055] The steps of the preparation method of the yellow slurry water are as follows:

[0056] (1) mixing and grinding with water after soaking dry soybean for 9h to obtain soybean milk; the mass volume ratio of described dry soybean and water is 3: 21g / ml;

[0057] (2) the soymilk obtained in step (1) is boiled for 12min and then cooled to 87°C, the soymilk after the cooling is mixed with a halogenating agent and left to stand for 25min to obtain bean curd, and the bean curd is squeezed to obtain yellow syrup; The mass ratio of halogen agent is 55:1.

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Abstract

The invention relates to the technical field of acetic bacteria culture, in particular to an acetic bacteria culture medium and a culture method of acetic bacteria. The acetic acid bacteria culture medium comprises yellow serofluid and genistein. The genistein is added into the yellow serofluid, and the yellow serofluid is used as an acetic acid bacteria culture medium, so that the growth of acetic acid bacteria can be remarkably promoted.

Description

technical field [0001] The invention relates to the technical field of acetic acid bacteria culture, in particular to an acetic acid bacteria culture medium and a method for culturing acetic bacteria. Background technique [0002] Acetobacter is one of the important industrial bacteria. Acetobacter cells are elliptical to rod-shaped; single, paired or chained; apical or peripheral hairs; motile or immobile; gram-negative; obligate aerobic bacteria, oxidizing various organic substances to form organic acids and other an oxide. [0003] An important feature of the genus Acetobacter is the ability to oxidize ethanol to acetic acid, and to oxidize acetic acid and lactic acid to CO 2 and H 2 O. Acetobacter is a prokaryotic organism that proliferates by binary fission. Acetobacter is widely distributed in nature, growing in the soil of orchards, on the surface of grapes or rancid food, and in unsterilized vinegar, cider and beer. The metabolic type belongs to the heterotroph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/02
CPCC12N1/20
Inventor 陈志娜王晓宇叶韬肖博雅
Owner HUAINAN NORMAL UNIV
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