44 results about "Bloodstream infection" patented technology

The invention discloses a severe patient organ failure assessment and risk stratification method based on the occurrence time point of blood flow infection, and relates to the field of severe medicine prognosis risk assessment modeling. Comprising the following steps: firstly, collecting daily sequential organ failure evaluation scores of a blood flow infection patient during an infection occurrence period, and dividing data into a training set and a verification set; importing the training set into a group trajectory model for fitting, determining the optimal grouping number of trajectory groups, and verifying the model through a verification set; then, according to a verification result, the patients are allocated to the track group with the highest membership probability for research; finally, a Cox proportional risk regression model is adopted, the correlation between different track groups and the death rate is evaluated, and decisive factors influencing prognosis are determined. According to the method, a robust framework is provided for hierarchical management of ICU blood flow infection patients, the death rate of the patients can be independently predicted, reference is provided for early intervention and formulation of personalized management strategies, and the clinical outcome of the patients is finally improved.

The invention provides a primer probe set for detecting candida bloodstream infection, a kit and application, and belongs to the technical field of microbiological detection. The primer probe group for detecting candida bloodstream infection comprises a primer probe group 1 and / or a primer probe group 2, the primer probe group 1 is one or more of a primer probe for detecting candida albicans, a primer probe for detecting candida tropicalis and a primer probe for detecting candida glabrata; and the primer probe group 2 is one or two of a primer probe for detecting candida krusei and a primer probe for detecting candida parapsilosis. The primer probe group 1 disclosed by the invention can be used for simultaneously and rapidly detecting candida albicans, candida tropicalis and candida glabrata; the primer probe group 2 can be used for simultaneously and rapidly detecting the candida krusei and the candida parapsilosis, and has the characteristics of high sensitivity, high detection specificity, simplicity and convenience in operation, short detection time and the like.

Catheter clips and catheter insertion assemblies including such catheter clips facilitate sterile catheterization technique, thereby reducing the risk of catheter-related bloodstream infections and increasing positive patient-centered outcomes. In an example, a catheter clip can include a pair of lever arms mated to each other. Each lever arm of the pair of lever arms can be bilaterally asymmetric but identical to the other lever arm of the pair of lever arms. An elastomeric band can be over a jaw end portion of the catheter clip. A catheter retainer can be coupled to a handle end portion of the catheter clip. The catheter retainer can be configured to hold a catheter connector of a catheter such that at least a proximal portion of the catheter remains in a sterile field when the catheter clip is clipped onto a sterile drape that establishes at least a portion of the sterile field.

The invention relates to the technical field of blood culture bottles, in particular to a composition and application thereof in preparation of a microbial culture medium. Yeast extract powder, soy peptone and casein tryptone are combined into a nitrogen source, and an aerobic and anaerobic culture medium for clinical pathogenic microorganism culture is formed. Through comparison verification, in growth experiments of nine aerobic bacteria and seven anaerobic bacteria, the culture speed and the biomass accumulation of the aerobic and anaerobic culture media added with the composition are integrally superior to those of other components excluded in the prior art and screening. Therefore, the composition can effectively promote the performance of a blood culture product, improve the clinical blood flow infection detection capability, inhibit antibiotic abuse and save the life of a patient.

The invention discloses a rapid detection method for bloodstream infection pathogenic bacteria based on a rapid mass spectrometry technology, and belongs to the technical field of medical detection. Analyzing the metabolic gas of the blood flow infected pathogenic bacteria by using UVP-TOFMS to obtain a pathogenic bacteria metabolic profile spectrogram; preprocessing the obtained pathogenic bacterium metabolism profile spectrogram data; integrating the preprocessed pathogenic bacterium metabolism profile spectrogram data by using a stacking algorithm, and constructing a pathogenic bacterium identification model; evaluating the effectiveness of the pathogenic bacterium identification model on a training set and a test set through accuracy, precision, a confusion matrix and a subject operation curve; and comparing and screening characteristic metabolic markers of the pathogenic bacteria by combining the importance and significance of model characteristics, and indicating the types of the pathogenic bacteria. The method does not need sample pretreatment, is simple and convenient to operate, short in detection time (20 seconds per sample), high in accuracy and suitable for clinical popularization, and provides technical support for early diagnosis and treatment of bloodstream infection.

The invention relates to the field of medical detection, in particular to a fluorescent sensing composition, a fluorescent sensing film and application thereof. According to the invention, by screening a plurality of fluorescent indicators and combinations thereof, an indicator formula with simple components and low cost is finally obtained. Based on the formula, by optimizing the preparation process, the high-efficiency fluorescent sensing film resistant to damp-heat sterilization is developed. After a microorganism culture bottle prepared on the basis of the membrane is inoculated with Gram-negative bacilli, positive cocci and yeast fungi representative quality control strains, experiments of full-automatic microorganism culture system fluorescence curve determination, Gram staining, microorganism mass spectrum identification and the like prove that the fluorescence sensing membrane is excellent in detection performance. The fluorescent sensing film provided by the research is applied to clinical microorganism detection, can provide technical support for blood flow infection diagnosis, and is of great significance in suppressing antibiotic abuse and improving prognosis of blood flow infection patients.

The invention provides a method and a kit for detecting blood flow infection pathogens and application of the method and the kit. Specifically, the invention provides a primer probe combination for detecting bloodstream infection pathogens, and the primer probe combination comprises: (a) upstream and downstream primers and probes for detecting serratia marcescens as shown in SEQ ID NO: 7-9; (b) an upstream primer, a downstream primer and a probe which are shown as SEQ ID NO: 10-12 and are used for detecting burkholderia cepacia; (c) an upstream primer, a downstream primer and a probe which are shown as SEQ ID NO: 1-3 and are used for detecting stenotrophomonas maltophilia; and (d) upstream and downstream primers and a probe which are shown as SEQ ID NO: 4-6 and are used for detecting enterobacter cloacae. The primer probe combination provided by the invention can be used for realizing rapid, high-sensitivity and high-specificity detection of blood flow infection pathogens.

The invention provides a method and a kit for detecting blood flow infection pathogens and application of the method and the kit. Specifically, the invention provides a primer probe combination for detecting bloodstream infection pathogens, and the primer probe combination comprises: (a) upstream and downstream primers and probes for detecting candida as shown in SEQ ID NO: 4-6; (b) upstream and downstream primers and probes for detecting candida glabrata as shown in SEQ ID NO: 1-3; (c) upstream and downstream primers and probes for detecting candida tropicalis as shown in SEQ ID NO: 7-9; and (d) an upstream primer, a downstream primer and a probe which are shown as SEQ ID NO: 10-12 and are used for detecting cryptococcus. The primer probe combination provided by the invention can be used for realizing rapid, high-sensitivity and high-specificity detection of blood flow infection pathogens.

The application provides a blood stream infection related septic shock multi-modal fusion prediction method and system, belonging to the field of intelligent medical treatment, and solving the problems of long time consumption, insufficient sensitivity and insufficient multi-modal information analysis capability in blood stream infection related septic shock. The method comprises: acquiring multi-modal clinical data of a target patient suspected of blood stream infection within a preset observation time window to obtain a multi-modal multi-scale feature sequence formed by combining each modality spatiotemporal feature vector sequence; inputting the multi-modal multi-scale feature sequence into a time sequence encoding network based on a multi-head attention mechanism to obtain an aligned multi-modal time sequence representation sequence; setting a learnable modality weight parameter for each modality, and updating the learnable modality weight parameter, the encoder and the time sequence encoding network through a task loss function according to the label of whether blood stream infection related septic shock occurs or not in the training process to obtain a multi-modal fusion model for prediction.

The invention belongs to the technical field of biology, and particularly relates to a reagent for detecting pathogenic genes as well as a preparation method and application thereof. The invention finds that a biological sample is enriched through broad-spectrum captured protein, the captured protein is labeled with biotin, pathogenic microorganisms are separated from the biological sample through high-density streptavidin coated magnetic beads, the pathogenic microorganisms are split after pure culture, pathogenic genes are detected after nucleic acid is extracted, and the turnover time is shortened to 4 hours. The precise distribution of nucleic acid is realized through an annular array reaction cavity preloaded with 32 freeze-dried LAMP reagents in combination with centrifugal force driving. The single chip synchronously detects more than or equal to 30 major pathogen genes / drug-resistant genes, covers common bloodstream infection pathogens, and breaks through the existing POCT flux bottleneck. According to the present invention, with the kit, the blood matrix inhibition can be eliminated, the pathogen concentration can be rapidly increased (the detection threshold value can be reached after culture), the positive detection rate can be increased to the clinical requirement level (gt, 90%), and the pathogen detection limit in the blood sample can meet the clinical critical infection early diagnosis requirement of less than or equal to 10 CFU / mL;

The invention relates to the technical field of computer vision, and discloses a blood culture specimen collection amount detection method and system based on computer vision, and the method comprises the following steps: image acquisition and three-dimensional attitude calculation; performing geometric correction on the image according to the three-dimensional attitude to obtain a corrected image; matching the corrected image with a preset bottle body geometric model, and performing liquid level identification and compensation according to a matching result; according to the compensation liquid level position and a preset bottle body geometric model, the blood sample volume is calculated, and the blood culture sample collection amount is output. According to the method, through a set of complete computer vision algorithm process, the limitation of a traditional manual method and the prior art in a complex actual scene is effectively overcome, the overall quality of blood culture is remarkably improved, and a reliable guarantee is provided for rapid and accurate diagnosis of blood flow infection.

Catheter clips (110) and catheter insertion assemblies including such catheter clips facilitate sterile catheterization, thereby reducing the risk of catheter-related bloodstream infections. A catheter clip (110) can include a pair of lever arms (142) mated to each other. Each lever arm of the pair of lever arms can be bilaterally asymmetric but identical to the other lever arm of the pair of lever arms. An elastomeric band (150) can be over a jaw end portion of the catheter clip. A catheter retainer (152) can be coupled to a handle end portion of the catheter clip. The catheter retainer can be configured to hold a catheter connector of a catheter such that at least a proximal portion of the catheter remains in a sterile field when the catheter clip is clipped onto a sterile drape that establishes at least a portion of the sterile field.

The invention belongs to the technical field of medical instruments, and particularly relates to a closed type indwelling catheter blood sampling device. According to the closed type indwelling catheter blood sampling device, the communication state of the three-way pipeline can be adjusted by rotating the switching valve, diluted blood is pumped out through the injection assembly, then the injection assembly is kept still, the communication state of the three-way pipeline is switched, and a blood sampling test tube for collecting blood needing to be inspected is inserted into the needle cover through the insertion opening in the needle cover; the head of the hollow needle head can be inserted into the blood collection test tube, blood needing to be inspected directly enters the blood collection test tube, and the blood collection test tube is taken down from the hollow needle head until the blood needing to be inspected is completely sucked; the communicating state of the three-way pipeline is switched again, the diluted blood in the injection assembly is injected back into the indwelling catheter, blood drawing work in the indwelling catheter is completed, the indwelling catheter does not need to be repeatedly connected and disconnected through a plurality of injectors in the whole process, the blood drawing step is simplified, and the risk of catheter related blood flow infection is reduced.

The invention discloses an artery indwelling needle, which belongs to the technical field of medical treatment, and comprises a joint and a catheter which are communicated with each other, the silica gel plug is arranged at the joint of the joint and the catheter; the pipe sealing liquid storage cavity is formed in the connector, and a first fluid channel is arranged between the pipe sealing liquid storage cavity and the connector in a communicating mode. According to the artery indwelling needle, the dynamic pressure balance cavity, the pressure sensitive piston, the communicating cavity, the balloon and the movable plate are arranged, catheter sealing operation and needle withdrawing action are synchronously completed, catheter blockage caused by manual operation delay is avoided, the artery indwelling needle is particularly suitable for patients needing long-term catheter indwelling, no open pipeline operation exists in the whole process, and the operation is convenient. The pre-filling sterile design of the tube sealing liquid cavity is combined, the incidence rate of related blood flow infection is effectively reduced, the whole process from blood drawing completion to tube sealing liquid injection does not need to disassemble an injector, switch a valve or manually connect the tube sealing liquid, the operation steps are reduced from traditional four steps to one step, and the operation time is effectively shortened.

The present invention relates to the field of bioengineering technology, and in particular to a biomarker for distinguishing Gram-positive cocci from Gram-negative bacilli in bloodstream infections in patients with sepsis. The present invention provides a biomarker that can distinguish bloodstream infections caused by different pathogens. Based on single-cell transcriptome sequencing of S100A12 expression levels in peripheral blood dendritic cells, this biomarker can be used to rapidly identify the type of sepsis infection in patients, providing a reference for early clinical intervention and treatment of sepsis.

The invention provides a hemodialysis catheter related blood flow infection risk screening system and method, and belongs to the technical field of medical management, and the system comprises an information input module which is used for inputting patient case information; the data acquisition module is used for acquiring multi-source data such as catheter indwelling operation records, catheter indwelling time and daily maintenance records; the risk screening module is used for screening catheter-related blood flow infection risks and determining risk levels; the evaluation module is used for evaluating the patient state and the catheter state; and the diagnosis judgment module is used for judging whether a catheter-related blood flow infection diagnosis standard is met or not and generating treatment intervention notification information. The method has the beneficial effects that by screening and determining the risk of blood flow infection related to the catheter, further evaluating the patient and catheter states, and combining an evaluation result, clinical manifestation and a microbiological examination result, judging whether an infection diagnosis standard is met and generating a treatment intervention notice, the screening efficiency is improved, and the infection occurrence rate is reduced; the treatment effect and prognosis of a patient are improved.

A novel anti-microbial coating and uses thereof on medical devices are presented. The novel coating is comprised of a layer of boron carbon nitride (BCN) coated onto a medical device such as a catheter. The BCN coating was found to inhibit bacterial growth by at least 80% and reduce biofilm formation by at least 60% on the treated surface of the catheter. This coating can be used to reduce or prevent central line associated bloodstream infections (CLABSI) in patients as well as inhibit bacterial growth and biofilm formation on a variety of medical devices or polymeric surfaces.

This invention discloses a low-infection-risk detachable fixation wing and grooveless stop clamp hemodialysis catheter system, belonging to the field of medical device technology. This invention addresses two core pain points of existing long-term hemodialysis catheters: first, it redesigns the traditional one-piece fixation wing as a detachable structure, allowing for quick assembly and disassembly by external force without damaging the catheter, facilitating skin cleaning and reducing the risk of local infection and mechanical damage; second, it optimizes the separate stop clamp into a grooveless structure integrated with the connector, employing a full, smooth inner lumen to completely eliminate dead zones for bacterial residue, reducing catheter-related bloodstream infections at the source. This invention has a simple structure, strong clinical adaptability, and can be widely applied in the field of long-term hemodialysis treatment, demonstrating significant clinical application value.

The invention discloses a shared sequence mediated enhanced single-tube nested multiplex PCR (Polymerase Chain Reaction) detection method and application, the method comprises the following steps: carrying out a first round of target amplification by utilizing a plurality of pairs of specific primers (F1 / R1-Fn / Rn) of which 5'ends are modified with the same shared sequence to generate an intermediate product containing the shared sequence; a single enhanced primer FR which is completely consistent with a shared sequence is adopted for second-round amplification, so that the detection sensitivity is remarkably improved. According to the method, single-tube nested multiplex PCR is innovatively realized through a shared sequence, the whole process is closed, pollution is avoided, and 3-10 kinds of targets can be synchronously detected. The method can realize absolute quantification less than 10 copies / [mu] L in a digital PCR system, and is especially suitable for high-sensitivity synchronous detection of blood flow infection pathogens and drug-resistant genes. The kit has high throughput of multiple PCR and high sensitivity of nested PCR, and has important application value in the fields of infectious disease diagnosis and drug-resistant gene screening.

The present invention provides a digital PCR kit for detecting blood stream infection pathogens. Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Escherichia coli, and Stenotrophomonas maltophilia are simultaneously detected in one tube of the kit. The buffer system of the kit includes Tris-HCl, glycerol, dNTPs, KCl, MgCl2, BSA, SSB, Taq DNA polymerase, UNG enzyme, primers, and probes. The volume concentration of glycerol in the buffer system is 4% - 8%, the mass-volume concentration of BSA in the buffer system is 0.2% - 0.8%, and the concentration of SSB in the buffer system is 2 - 4 ng / µL. In the buffer system of the present invention, the droplet stability is improved, the amplification efficiency is improved, low-concentration and high-concentration targets can be stably detected, and when a large volume of nucleic acid template is added, the droplets are stable and the amplification efficiency is not affected, meeting the clinical use effect.

The invention discloses a primer group for detecting free nucleic acid of a blood flow infection pathogen, a kit and application. The primer group comprises 19 groups of primers, trace and fragmented pathogen cfDNA in a blood sample can be subjected to targeted linear enrichment through the primer group, and then synchronous detection of multiple pathogens and drug-resistant genes is performed in combination with high-throughput sequencing. And finally, synchronous identification of 15 clinical important bloodstream infection pathogens and 4 drug-resistant genes can be realized within a relatively short time (about 12 hours), the method has high sensitivity and high specificity, the detection limit can reach 0.1 copy / mL, the detection cost and the operation difficulty are greatly reduced, and the method is suitable for clinical popularization and application.

Described herein are methods for preventing microbial infections derived from an implanted catheters when not in use. In one aspect, the method involves filing the implanted catheter when not in use with a lock solution comprising a pharmaceutically acceptable salt of methanetrisdiazeniumdiolate and a pharmaceutically acceptable carrier. The lock solution releases nitric oxide and Azanone, which makes the lock solution a highly effective antimicrobial agent. The lock solution and use thereof can be effective in reducing or preventing particularly dangerous infections such as, for example, bloodstream infections.

The invention provides a method and a kit for detecting blood flow infection pathogens and application of the method and the kit. Specifically, the invention provides a primer probe combination for detecting bloodstream infection pathogens, and the primer probe combination comprises: (a) upstream and downstream primers and probes for detecting serratia marcescens as shown in SEQ ID NO: 7-9; (b) an upstream primer, a downstream primer and a probe which are shown as SEQ ID NO: 10-12 and are used for detecting burkholderia cepacia; (c) an upstream primer, a downstream primer and a probe for detecting stenotrophomonas maltophilia as shown in SEQ ID NO: 1-3; and (d) upstream and downstream primers and a probe which are shown as SEQ ID NO: 4-6 and are used for detecting enterobacter cloacae. The primer probe combination provided by the invention can be used for realizing rapid, high-sensitivity and high-specificity detection of blood flow infection pathogens.

The invention relates to the field of medical detection, in particular to a colorimetric sensing composition, a colorimetric sensing film and application of the colorimetric sensing film. According to the invention, a domestic indicator is adopted, and the colorimetric sensing film resistant to damp-heat sterilization is developed through a simple preparation process. The sensing membrane has the characteristics of simple preparation process, low cost, stable performance and the like, and can effectively meet clinical detection requirements. The preparation method provided by the research is simple, convenient and effective, is expected to provide technical support for increasing the market proportion of domestic blood culture products and improving the blood flow infection diagnosis efficiency, and has positive significance for promoting reasonable medication and improving the treatment success rate.

The invention provides a bloodstream infection risk prediction device based on multi-scale attention feature enhancement. The bloodstream infection risk prediction device comprises a memory and a risk early warning device. The memory stores user physiological index data and a computer program, the processor reads the data and operates the program to realize risk prediction, and the early warning device executes graded early warning according to a prediction result. The prediction process comprises a data preprocessing (physiological index feature dimension expansion) module and a three-level series model module. A standardization processing module normalizes preprocessed data to eliminate dimension differences. The feature enhancement module is used for jointly mining multivariable interaction relations and cross-patient group similarity information in individual moments through multi-branch collaboration (linear transformation, a self-attention mechanism and a Mama layer), and effectively capturing multi-scale dependence of physiological indexes; and the linear prediction module outputs a risk value through a full connection layer with Softmax. The device solves the problems of single-dimension processing, insufficient multi-scale dependence capture and high missed and false alarm rate in the prior art, the prediction stability is improved, the early accurate early warning requirement of blood flow infection in an ICU high-risk scene is met, and clinical advanced intervention is assisted to improve the prognosis of a patient.

Disclosed are patch devices, methods, kits and systems for reducing the incidence of infections associated with the insertion site of a transdermal medical device (e.g., blood stream infections, infections of the skin opening through which the device enters the body, CLABSI, CRBSI) for example, the insertion site where a cannula or catheter enters a living body by generating an electromagnetic antiseptic signal in proximity of the insertion site.

Provided herein are biomarkers that are useful in various aspects of treating and preventing complications and side effects associated with a disease or disorder, such as those associated with immune suppression, disruption of the gastrointestinal microbiome, infections (e.g., bloodstream infections), and / or GvHD, for example, in HSCT subjects. In some aspects, the biomarkers provided herein can be used in identifying a subject who is responding to a bacterial composition and / or subjects that are suitable for a treatment with a bacterial composition.

The application discloses a kit and a method for detecting blood stream infection bacterial nucleic acid by using a multiple probe melting curve method, and utilizes a multiple probe melting curve technology to adopt four different fluorescent probes to carry out fluorescent PCR combined with multiple melting curves, and develops an eight-target bacterial detection kit for common eight kinds of bacteria in blood stream infection. The application has the sensitivity and the specificity of the fluorescent quantitative PCR, and has a larger detection flux than the fluorescent quantitative PCR method, is more convenient to operate, and is lower in cost.

The invention provides a primer probe combination and a kit for detecting bloodstream infection pathogens and application of the primer probe combination and the kit. Specifically, the invention provides a primer probe combination for detecting blood flow infection pathogens, and the primer probe combination comprises: (a) upstream and downstream primers and probes for detecting pseudomonas aeruginosa as shown in SEQ ID NO: 1-3; (b) upstream and downstream primers and probes for detecting acinetobacter baumannii as shown in SEQ ID NO: 4-6; (c) an upstream primer, a downstream primer and a probe which are shown as SEQ ID NO: 7-9 and are used for detecting enterococcus; and (d) upstream and downstream primers and a probe which are shown as SEQ ID NO: 10-12 and are used for detecting streptococcus. The primer probe combination provided by the invention can be used for realizing rapid, high-sensitivity and high-specificity detection of blood flow infection pathogens.

The utility model relates to the technical field of medical instruments, in particular to a non-injury needle for an infusion port, which comprises a puncture needle, a plurality of needle holes are arranged at one end of the puncture needle far away from a needle handle, and the needle holes are arranged around the puncture needle. During tube sealing, tube sealing liquid can flush the liquid storage groove of the injection seat from multiple directions through the needle holes of the infusion port non-injury needle, a puncture needle does not need to be rotated, the operation time of a nurse is shortened, pain of a patient is reduced, meanwhile, the tube sealing effect is improved, and the tube blocking probability is reduced; complications such as thrombosis and catheter-related blood flow infection are reduced.