63 results about "Bloodstream infection" patented technology

The invention belongs to the field of molecular biological detection, and provides a joint detection composition which is mainly used for detecting related pathogens by using a multiple fluorescent PCR (Polymerase Chain Reaction) analysis method. Therefore, at least four of staphylococcus aureus, staphylococcus capitis, bacteroides fragilis, streptococcus agalactiae, streptococcus salivarius and staphylococcus hominis are detected and distinguished in a single-tube reaction system at the same time. Meanwhile, the detection sensitivity of the composition is higher and reaches 200 copies / mL, and the detection is more accurate.

The invention discloses a severe patient organ failure assessment and risk stratification method based on the occurrence time point of blood flow infection, and relates to the field of severe medicine prognosis risk assessment modeling. Comprising the following steps: firstly, collecting daily sequential organ failure evaluation scores of a blood flow infection patient during an infection occurrence period, and dividing data into a training set and a verification set; importing the training set into a group trajectory model for fitting, determining the optimal grouping number of trajectory groups, and verifying the model through a verification set; then, according to a verification result, the patients are allocated to the track group with the highest membership probability for research; finally, a Cox proportional risk regression model is adopted, the correlation between different track groups and the death rate is evaluated, and decisive factors influencing prognosis are determined. According to the method, a robust framework is provided for hierarchical management of ICU blood flow infection patients, the death rate of the patients can be independently predicted, reference is provided for early intervention and formulation of personalized management strategies, and the clinical outcome of the patients is finally improved.

The invention belongs to the field of molecular biological detection, and provides a joint detection composition which is mainly used for detecting related pathogens by using a multiple fluorescent PCR (Polymerase Chain Reaction) analysis method. Therefore, at least four of the klebsiella aerogenes, the stenotrophomonas maltophilia, the streptococcus angina, the acinetobacter baumannii, the klebsiella pneumoniae and the klebsiella oxytoca can be simultaneously detected and distinguished in a single-tube reaction system. Meanwhile, the detection sensitivity of the composition is higher and reaches 500 copies / mL, and the detection is more accurate.

The invention provides a primer probe set for detecting candida bloodstream infection, a kit and application, and belongs to the technical field of microbiological detection. The primer probe group for detecting candida bloodstream infection comprises a primer probe group 1 and / or a primer probe group 2, the primer probe group 1 is one or more of a primer probe for detecting candida albicans, a primer probe for detecting candida tropicalis and a primer probe for detecting candida glabrata; and the primer probe group 2 is one or two of a primer probe for detecting candida krusei and a primer probe for detecting candida parapsilosis. The primer probe group 1 disclosed by the invention can be used for simultaneously and rapidly detecting candida albicans, candida tropicalis and candida glabrata; the primer probe group 2 can be used for simultaneously and rapidly detecting the candida krusei and the candida parapsilosis, and has the characteristics of high sensitivity, high detection specificity, simplicity and convenience in operation, short detection time and the like.

Catheter clips and catheter insertion assemblies including such catheter clips facilitate sterile catheterization technique, thereby reducing the risk of catheter-related bloodstream infections and increasing positive patient-centered outcomes. In an example, a catheter clip can include a pair of lever arms mated to each other. Each lever arm of the pair of lever arms can be bilaterally asymmetric but identical to the other lever arm of the pair of lever arms. An elastomeric band can be over a jaw end portion of the catheter clip. A catheter retainer can be coupled to a handle end portion of the catheter clip. The catheter retainer can be configured to hold a catheter connector of a catheter such that at least a proximal portion of the catheter remains in a sterile field when the catheter clip is clipped onto a sterile drape that establishes at least a portion of the sterile field.

The invention belongs to the field of molecular biology detection, particularly relates to detection of blood flow infection related pathogens, and more particularly relates to detection and distinguishing of at least four pathogens in burkholderia cepacia, streptococcus mirabilis, staphylococcus capitis, staphylococcus coriolis, proteus mirabilis and staphylococcus haemolyticus. According to the combined detection composition provided by the invention, different pathogens are detected by mainly utilizing a multiple fluorescent PCR analysis method and detecting target spots on the different pathogens; therefore, at least four pathogens in burkholderia cepacia, streptococcus mitis, staphylococcus capitis, staphylococcus coriolis, proteus mirabilis and staphylococcus haemolyticus are detected and distinguished in a single-tube reaction system at the same time, and the detection efficiency is improved. The composition disclosed by the invention is high in detection sensitivity, and the detection sensitivity reaches 200 copies / mL; the reagent / kit provided by the invention adopts a UNG enzyme and dUTP anti-pollution system, so that the accuracy of the detection effect can be guaranteed.

The invention relates to the technical field of blood culture bottles, in particular to a composition and application thereof in preparation of a microbial culture medium. Yeast extract powder, soy peptone and casein tryptone are combined into a nitrogen source, and an aerobic and anaerobic culture medium for clinical pathogenic microorganism culture is formed. Through comparison verification, in growth experiments of nine aerobic bacteria and seven anaerobic bacteria, the culture speed and the biomass accumulation of the aerobic and anaerobic culture media added with the composition are integrally superior to those of other components excluded in the prior art and screening. Therefore, the composition can effectively promote the performance of a blood culture product, improve the clinical blood flow infection detection capability, inhibit antibiotic abuse and save the life of a patient.

The invention discloses a rapid detection method for bloodstream infection pathogenic bacteria based on a rapid mass spectrometry technology, and belongs to the technical field of medical detection. Analyzing the metabolic gas of the blood flow infected pathogenic bacteria by using UVP-TOFMS to obtain a pathogenic bacteria metabolic profile spectrogram; preprocessing the obtained pathogenic bacterium metabolism profile spectrogram data; integrating the preprocessed pathogenic bacterium metabolism profile spectrogram data by using a stacking algorithm, and constructing a pathogenic bacterium identification model; evaluating the effectiveness of the pathogenic bacterium identification model on a training set and a test set through accuracy, precision, a confusion matrix and a subject operation curve; and comparing and screening characteristic metabolic markers of the pathogenic bacteria by combining the importance and significance of model characteristics, and indicating the types of the pathogenic bacteria. The method does not need sample pretreatment, is simple and convenient to operate, short in detection time (20 seconds per sample), high in accuracy and suitable for clinical popularization, and provides technical support for early diagnosis and treatment of bloodstream infection.

The invention relates to the field of medical detection, in particular to a fluorescent sensing composition, a fluorescent sensing film and application thereof. According to the invention, by screening a plurality of fluorescent indicators and combinations thereof, an indicator formula with simple components and low cost is finally obtained. Based on the formula, by optimizing the preparation process, the high-efficiency fluorescent sensing film resistant to damp-heat sterilization is developed. After a microorganism culture bottle prepared on the basis of the membrane is inoculated with Gram-negative bacilli, positive cocci and yeast fungi representative quality control strains, experiments of full-automatic microorganism culture system fluorescence curve determination, Gram staining, microorganism mass spectrum identification and the like prove that the fluorescence sensing membrane is excellent in detection performance. The fluorescent sensing film provided by the research is applied to clinical microorganism detection, can provide technical support for blood flow infection diagnosis, and is of great significance in suppressing antibiotic abuse and improving prognosis of blood flow infection patients.

The invention provides a method and a kit for detecting blood flow infection pathogens and application of the method and the kit. Specifically, the invention provides a primer probe combination for detecting bloodstream infection pathogens, and the primer probe combination comprises: (a) upstream and downstream primers and probes for detecting serratia marcescens as shown in SEQ ID NO: 7-9; (b) an upstream primer, a downstream primer and a probe which are shown as SEQ ID NO: 10-12 and are used for detecting burkholderia cepacia; (c) an upstream primer, a downstream primer and a probe which are shown as SEQ ID NO: 1-3 and are used for detecting stenotrophomonas maltophilia; and (d) upstream and downstream primers and a probe which are shown as SEQ ID NO: 4-6 and are used for detecting enterobacter cloacae. The primer probe combination provided by the invention can be used for realizing rapid, high-sensitivity and high-specificity detection of blood flow infection pathogens.

The invention provides a method and a kit for detecting blood flow infection pathogens and application of the method and the kit. Specifically, the invention provides a primer probe combination for detecting bloodstream infection pathogens, and the primer probe combination comprises: (a) upstream and downstream primers and probes for detecting candida as shown in SEQ ID NO: 4-6; (b) upstream and downstream primers and probes for detecting candida glabrata as shown in SEQ ID NO: 1-3; (c) upstream and downstream primers and probes for detecting candida tropicalis as shown in SEQ ID NO: 7-9; and (d) an upstream primer, a downstream primer and a probe which are shown as SEQ ID NO: 10-12 and are used for detecting cryptococcus. The primer probe combination provided by the invention can be used for realizing rapid, high-sensitivity and high-specificity detection of blood flow infection pathogens.

The utility model relates to the technical field of silver-containing dressings, and discloses a silver-containing dressing for preventing central venous catheter-related bloodstream infection in burn patients. The utility model comprises a base cloth block, a silver-containing dressing sheet is fixedly connected to the base cloth block below, four sides of the base cloth block are connected to base cloth strips through cut point break lines, base cloth corners are connected between the base cloth strips through cut point break lines, adhesive layers are connected to the base cloth strips and the base cloth corners below, a connecting sheet is fixedly connected to the top of the base cloth block through a plastic film sheet, and a plurality of suspended adhesive tape bodies are fixedly connected to the edge of the connecting sheet in a circumferentially equidistant array. The silver-containing dressing for preventing central venous catheter-related bloodstream infection in burn patients disclosed by the utility model can be spliced ​​to each other into a desired area size according to needs, and the seams at the splicing are neat, the user is more comfortable, and the connection between the silver-containing dressing unit sheets can be strengthened by the suspended adhesive tape body, and the connection is stable and not easy to fall apart.

The invention provides a central catheter related blood flow infection prevention and control management system and method, electronic equipment and a computer readable storage medium, and relates to the field of medical information management, and the system comprises a case management module, a medical care behavior examination module and a monitoring module; wherein the case management module is used for managing case information in a case storage library; the medical care behavior review module comprises a plurality of review sub-modules, and each review sub-module is used for acquiring an index value of a corresponding behavior index and recording the index value into the case information; the monitoring module comprises a basic index monitoring sub-module and an early warning sub-module; the basic index monitoring sub-module is used for collecting the case information of a patient in real time; monitoring whether the patient has a risk index or not, and generating a monitoring result; and the early warning sub-module is used for determining a high-risk patient with blood flow infection related to the central catheter in combination with the monitoring result and a risk evaluation strategy, and performing early warning reminding.

The application provides a blood stream infection related septic shock multi-modal fusion prediction method and system, belonging to the field of intelligent medical treatment, and solving the problems of long time consumption, insufficient sensitivity and insufficient multi-modal information analysis capability in blood stream infection related septic shock. The method comprises: acquiring multi-modal clinical data of a target patient suspected of blood stream infection within a preset observation time window to obtain a multi-modal multi-scale feature sequence formed by combining each modality spatiotemporal feature vector sequence; inputting the multi-modal multi-scale feature sequence into a time sequence encoding network based on a multi-head attention mechanism to obtain an aligned multi-modal time sequence representation sequence; setting a learnable modality weight parameter for each modality, and updating the learnable modality weight parameter, the encoder and the time sequence encoding network through a task loss function according to the label of whether blood stream infection related septic shock occurs or not in the training process to obtain a multi-modal fusion model for prediction.

A novel anti-microbial coating and uses thereof on medical devices are presented. The novel coating is comprised of a layer of boron carbon nitride (BCN) coated onto a medical device such as a catheter. The BCN coating was found to inhibit bacterial growth by at least 80% and reduce biofilm formation by at least 60% on the treated surface of the catheter. This coating can be used to reduce or prevent central line associated bloodstream infections (CLABSI) in patients as well as inhibit bacterial growth and biofilm formation on a variety of medical devices or polymeric surfaces.

The invention belongs to the technical field of biology, and particularly relates to a reagent for detecting pathogenic genes as well as a preparation method and application thereof. The invention finds that a biological sample is enriched through broad-spectrum captured protein, the captured protein is labeled with biotin, pathogenic microorganisms are separated from the biological sample through high-density streptavidin coated magnetic beads, the pathogenic microorganisms are split after pure culture, pathogenic genes are detected after nucleic acid is extracted, and the turnover time is shortened to 4 hours. The precise distribution of nucleic acid is realized through an annular array reaction cavity preloaded with 32 freeze-dried LAMP reagents in combination with centrifugal force driving. The single chip synchronously detects more than or equal to 30 major pathogen genes / drug-resistant genes, covers common bloodstream infection pathogens, and breaks through the existing POCT flux bottleneck. According to the present invention, with the kit, the blood matrix inhibition can be eliminated, the pathogen concentration can be rapidly increased (the detection threshold value can be reached after culture), the positive detection rate can be increased to the clinical requirement level (gt, 90%), and the pathogen detection limit in the blood sample can meet the clinical critical infection early diagnosis requirement of less than or equal to 10 CFU / mL;

The invention relates to the technical field of computer vision, and discloses a blood culture specimen collection amount detection method and system based on computer vision, and the method comprises the following steps: image acquisition and three-dimensional attitude calculation; performing geometric correction on the image according to the three-dimensional attitude to obtain a corrected image; matching the corrected image with a preset bottle body geometric model, and performing liquid level identification and compensation according to a matching result; according to the compensation liquid level position and a preset bottle body geometric model, the blood sample volume is calculated, and the blood culture sample collection amount is output. According to the method, through a set of complete computer vision algorithm process, the limitation of a traditional manual method and the prior art in a complex actual scene is effectively overcome, the overall quality of blood culture is remarkably improved, and a reliable guarantee is provided for rapid and accurate diagnosis of blood flow infection.

Catheter clips (110) and catheter insertion assemblies including such catheter clips facilitate sterile catheterization, thereby reducing the risk of catheter-related bloodstream infections. A catheter clip (110) can include a pair of lever arms (142) mated to each other. Each lever arm of the pair of lever arms can be bilaterally asymmetric but identical to the other lever arm of the pair of lever arms. An elastomeric band (150) can be over a jaw end portion of the catheter clip. A catheter retainer (152) can be coupled to a handle end portion of the catheter clip. The catheter retainer can be configured to hold a catheter connector of a catheter such that at least a proximal portion of the catheter remains in a sterile field when the catheter clip is clipped onto a sterile drape that establishes at least a portion of the sterile field.

The invention belongs to the technical field of medical instruments, and particularly relates to a closed type indwelling catheter blood sampling device. According to the closed type indwelling catheter blood sampling device, the communication state of the three-way pipeline can be adjusted by rotating the switching valve, diluted blood is pumped out through the injection assembly, then the injection assembly is kept still, the communication state of the three-way pipeline is switched, and a blood sampling test tube for collecting blood needing to be inspected is inserted into the needle cover through the insertion opening in the needle cover; the head of the hollow needle head can be inserted into the blood collection test tube, blood needing to be inspected directly enters the blood collection test tube, and the blood collection test tube is taken down from the hollow needle head until the blood needing to be inspected is completely sucked; the communicating state of the three-way pipeline is switched again, the diluted blood in the injection assembly is injected back into the indwelling catheter, blood drawing work in the indwelling catheter is completed, the indwelling catheter does not need to be repeatedly connected and disconnected through a plurality of injectors in the whole process, the blood drawing step is simplified, and the risk of catheter related blood flow infection is reduced.

The invention discloses an artery indwelling needle, which belongs to the technical field of medical treatment, and comprises a joint and a catheter which are communicated with each other, the silica gel plug is arranged at the joint of the joint and the catheter; the pipe sealing liquid storage cavity is formed in the connector, and a first fluid channel is arranged between the pipe sealing liquid storage cavity and the connector in a communicating mode. According to the artery indwelling needle, the dynamic pressure balance cavity, the pressure sensitive piston, the communicating cavity, the balloon and the movable plate are arranged, catheter sealing operation and needle withdrawing action are synchronously completed, catheter blockage caused by manual operation delay is avoided, the artery indwelling needle is particularly suitable for patients needing long-term catheter indwelling, no open pipeline operation exists in the whole process, and the operation is convenient. The pre-filling sterile design of the tube sealing liquid cavity is combined, the incidence rate of related blood flow infection is effectively reduced, the whole process from blood drawing completion to tube sealing liquid injection does not need to disassemble an injector, switch a valve or manually connect the tube sealing liquid, the operation steps are reduced from traditional four steps to one step, and the operation time is effectively shortened.

The present invention relates to the field of bioengineering technology, and in particular to a biomarker for distinguishing Gram-positive cocci from Gram-negative bacilli in bloodstream infections in patients with sepsis. The present invention provides a biomarker that can distinguish bloodstream infections caused by different pathogens. Based on single-cell transcriptome sequencing of S100A12 expression levels in peripheral blood dendritic cells, this biomarker can be used to rapidly identify the type of sepsis infection in patients, providing a reference for early clinical intervention and treatment of sepsis.

The present invention discloses a method, a kit and a use of emQPCR multi-target joint detection of trace DNA, the method comprising the following steps: 1) emulsion microdroplet nested multiple PCR pre-amplification, preparing a water phase and an oil phase in a certain ratio to generate an oil-in-water microdroplet system, and then performing 10-20 cycles of nested PCR pre-amplification on the prepared microdroplet system; after the microdroplet pre-amplification, the emulsion is broken to obtain a pre-amplification product of the water phase; 2) multiple nested real-time fluorescent PCR amplification detection, using a set of "nested" primer probes for each detection target, and realizing a highly specific single-tube multiple first-round nested pre-amplification and a second-round fluorescent PCR amplification multiple joint detection through this set of "nested" primer probes. The present invention provides a highly sensitive, effective and rapid molecular detection scheme for the detection of pathogens such as bloodstream infection and bone and joint infection.

The invention provides a hemodialysis catheter related blood flow infection risk screening system and method, and belongs to the technical field of medical management, and the system comprises an information input module which is used for inputting patient case information; the data acquisition module is used for acquiring multi-source data such as catheter indwelling operation records, catheter indwelling time and daily maintenance records; the risk screening module is used for screening catheter-related blood flow infection risks and determining risk levels; the evaluation module is used for evaluating the patient state and the catheter state; and the diagnosis judgment module is used for judging whether a catheter-related blood flow infection diagnosis standard is met or not and generating treatment intervention notification information. The method has the beneficial effects that by screening and determining the risk of blood flow infection related to the catheter, further evaluating the patient and catheter states, and combining an evaluation result, clinical manifestation and a microbiological examination result, judging whether an infection diagnosis standard is met and generating a treatment intervention notice, the screening efficiency is improved, and the infection occurrence rate is reduced; the treatment effect and prognosis of a patient are improved.

A novel anti-microbial coating and uses thereof on medical devices are presented. The novel coating is comprised of a layer of boron carbon nitride (BCN) coated onto a medical device such as a catheter. The BCN coating was found to inhibit bacterial growth by at least 80% and reduce biofilm formation by at least 60% on the treated surface of the catheter. This coating can be used to reduce or prevent central line associated bloodstream infections (CLABSI) in patients as well as inhibit bacterial growth and biofilm formation on a variety of medical devices or polymeric surfaces.

This invention discloses a low-infection-risk detachable fixation wing and grooveless stop clamp hemodialysis catheter system, belonging to the field of medical device technology. This invention addresses two core pain points of existing long-term hemodialysis catheters: first, it redesigns the traditional one-piece fixation wing as a detachable structure, allowing for quick assembly and disassembly by external force without damaging the catheter, facilitating skin cleaning and reducing the risk of local infection and mechanical damage; second, it optimizes the separate stop clamp into a grooveless structure integrated with the connector, employing a full, smooth inner lumen to completely eliminate dead zones for bacterial residue, reducing catheter-related bloodstream infections at the source. This invention has a simple structure, strong clinical adaptability, and can be widely applied in the field of long-term hemodialysis treatment, demonstrating significant clinical application value.

The invention discloses a shared sequence mediated enhanced single-tube nested multiplex PCR (Polymerase Chain Reaction) detection method and application, the method comprises the following steps: carrying out a first round of target amplification by utilizing a plurality of pairs of specific primers (F1 / R1-Fn / Rn) of which 5'ends are modified with the same shared sequence to generate an intermediate product containing the shared sequence; a single enhanced primer FR which is completely consistent with a shared sequence is adopted for second-round amplification, so that the detection sensitivity is remarkably improved. According to the method, single-tube nested multiplex PCR is innovatively realized through a shared sequence, the whole process is closed, pollution is avoided, and 3-10 kinds of targets can be synchronously detected. The method can realize absolute quantification less than 10 copies / [mu] L in a digital PCR system, and is especially suitable for high-sensitivity synchronous detection of blood flow infection pathogens and drug-resistant genes. The kit has high throughput of multiple PCR and high sensitivity of nested PCR, and has important application value in the fields of infectious disease diagnosis and drug-resistant gene screening.

The invention belongs to the field of biological detection. The invention provides a composition for detecting bloodstream infection pathogens based on high-throughput sequencing. Comprising staphylococcus aureus, streptococcus pneumoniae, human herpes B virus type 5, staphylococcus epidermidis, streptococcus agalactiae, orientia tsutsugamushi, coxiella burnetii, chlamydia psittaci, human herpes virus type 2, human herpes virus type 4, human herpes virus type 6, candida tropicalis, enterococcus avium, staphylococcus haemolyticus, streptococcus intermedius and plasmodium vivax. And at least 4 targets in 32 blood flow infection pathogens such as human adenovirus type C, escherichia coli, torque teno virus, corynebacterium striatum and the like. By using the composition disclosed by the invention, a plurality of targets can be amplified and enriched in one tube at the same time, so that the data volume required by subsequent sequencing is remarkably reduced, the analysis work is simplified, and the whole detection is more accurate and efficient.

The present invention provides a digital PCR kit for detecting blood stream infection pathogens. Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Escherichia coli, and Stenotrophomonas maltophilia are simultaneously detected in one tube of the kit. The buffer system of the kit includes Tris-HCl, glycerol, dNTPs, KCl, MgCl2, BSA, SSB, Taq DNA polymerase, UNG enzyme, primers, and probes. The volume concentration of glycerol in the buffer system is 4% - 8%, the mass-volume concentration of BSA in the buffer system is 0.2% - 0.8%, and the concentration of SSB in the buffer system is 2 - 4 ng / µL. In the buffer system of the present invention, the droplet stability is improved, the amplification efficiency is improved, low-concentration and high-concentration targets can be stably detected, and when a large volume of nucleic acid template is added, the droplets are stable and the amplification efficiency is not affected, meeting the clinical use effect.

The invention discloses a primer probe and a kit for rapidly detecting common candida of blood flow infection and application, and particularly relates to a candida universal primer and a Taqman-MGB probe which are required for implementing a fluorescent quantitative PCR (Polymerase Chain Reaction) technology, as well as a red blood cell lysis buffer, a white blood cell lysis buffer and the like. The lysate can efficiently lyse red blood cells and white blood cells in peripheral blood, and the primers and the probes can specifically amplify DNA of candida albicans, candida glabrata, candida parapsilosis, candida tropicalis and candida krusei. The kit takes whole blood as a detection sample and has the characteristics of high sensitivity, good precision, high detection speed and the like, so that early diagnosis and timely treatment of diseases are assisted.

The invention discloses a primer group for detecting free nucleic acid of a blood flow infection pathogen, a kit and application. The primer group comprises 19 groups of primers, trace and fragmented pathogen cfDNA in a blood sample can be subjected to targeted linear enrichment through the primer group, and then synchronous detection of multiple pathogens and drug-resistant genes is performed in combination with high-throughput sequencing. And finally, synchronous identification of 15 clinical important bloodstream infection pathogens and 4 drug-resistant genes can be realized within a relatively short time (about 12 hours), the method has high sensitivity and high specificity, the detection limit can reach 0.1 copy / mL, the detection cost and the operation difficulty are greatly reduced, and the method is suitable for clinical popularization and application.