13 results about "Bloodstream infection" patented technology

The application provides a blood stream infection related septic shock multi-modal fusion prediction method and system, belonging to the field of intelligent medical treatment, and solving the problems of long time consumption, insufficient sensitivity and insufficient multi-modal information analysis capability in blood stream infection related septic shock. The method comprises: acquiring multi-modal clinical data of a target patient suspected of blood stream infection within a preset observation time window to obtain a multi-modal multi-scale feature sequence formed by combining each modality spatiotemporal feature vector sequence; inputting the multi-modal multi-scale feature sequence into a time sequence encoding network based on a multi-head attention mechanism to obtain an aligned multi-modal time sequence representation sequence; setting a learnable modality weight parameter for each modality, and updating the learnable modality weight parameter, the encoder and the time sequence encoding network through a task loss function according to the label of whether blood stream infection related septic shock occurs or not in the training process to obtain a multi-modal fusion model for prediction.

The invention belongs to the technical field of biology, and particularly relates to a reagent for detecting pathogenic genes as well as a preparation method and application thereof. The invention finds that a biological sample is enriched through broad-spectrum captured protein, the captured protein is labeled with biotin, pathogenic microorganisms are separated from the biological sample through high-density streptavidin coated magnetic beads, the pathogenic microorganisms are split after pure culture, pathogenic genes are detected after nucleic acid is extracted, and the turnover time is shortened to 4 hours. The precise distribution of nucleic acid is realized through an annular array reaction cavity preloaded with 32 freeze-dried LAMP reagents in combination with centrifugal force driving. The single chip synchronously detects more than or equal to 30 major pathogen genes / drug-resistant genes, covers common bloodstream infection pathogens, and breaks through the existing POCT flux bottleneck. According to the present invention, with the kit, the blood matrix inhibition can be eliminated, the pathogen concentration can be rapidly increased (the detection threshold value can be reached after culture), the positive detection rate can be increased to the clinical requirement level (gt, 90%), and the pathogen detection limit in the blood sample can meet the clinical critical infection early diagnosis requirement of less than or equal to 10 CFU / mL;

The invention relates to the technical field of computer vision, and discloses a blood culture specimen collection amount detection method and system based on computer vision, and the method comprises the following steps: image acquisition and three-dimensional attitude calculation; performing geometric correction on the image according to the three-dimensional attitude to obtain a corrected image; matching the corrected image with a preset bottle body geometric model, and performing liquid level identification and compensation according to a matching result; according to the compensation liquid level position and a preset bottle body geometric model, the blood sample volume is calculated, and the blood culture sample collection amount is output. According to the method, through a set of complete computer vision algorithm process, the limitation of a traditional manual method and the prior art in a complex actual scene is effectively overcome, the overall quality of blood culture is remarkably improved, and a reliable guarantee is provided for rapid and accurate diagnosis of blood flow infection.

This invention discloses a low-infection-risk detachable fixation wing and grooveless stop clamp hemodialysis catheter system, belonging to the field of medical device technology. This invention addresses two core pain points of existing long-term hemodialysis catheters: first, it redesigns the traditional one-piece fixation wing as a detachable structure, allowing for quick assembly and disassembly by external force without damaging the catheter, facilitating skin cleaning and reducing the risk of local infection and mechanical damage; second, it optimizes the separate stop clamp into a grooveless structure integrated with the connector, employing a full, smooth inner lumen to completely eliminate dead zones for bacterial residue, reducing catheter-related bloodstream infections at the source. This invention has a simple structure, strong clinical adaptability, and can be widely applied in the field of long-term hemodialysis treatment, demonstrating significant clinical application value.

The invention provides a bloodstream infection risk prediction device based on multi-scale attention feature enhancement. The bloodstream infection risk prediction device comprises a memory and a risk early warning device. The memory stores user physiological index data and a computer program, the processor reads the data and operates the program to realize risk prediction, and the early warning device executes graded early warning according to a prediction result. The prediction process comprises a data preprocessing (physiological index feature dimension expansion) module and a three-level series model module. A standardization processing module normalizes preprocessed data to eliminate dimension differences. The feature enhancement module is used for jointly mining multivariable interaction relations and cross-patient group similarity information in individual moments through multi-branch collaboration (linear transformation, a self-attention mechanism and a Mama layer), and effectively capturing multi-scale dependence of physiological indexes; and the linear prediction module outputs a risk value through a full connection layer with Softmax. The device solves the problems of single-dimension processing, insufficient multi-scale dependence capture and high missed and false alarm rate in the prior art, the prediction stability is improved, the early accurate early warning requirement of blood flow infection in an ICU high-risk scene is met, and clinical advanced intervention is assisted to improve the prognosis of a patient.

Disclosed are patch devices, methods, kits and systems for reducing the incidence of infections associated with the insertion site of a transdermal medical device (e.g., blood stream infections, infections of the skin opening through which the device enters the body, CLABSI, CRBSI) for example, the insertion site where a cannula or catheter enters a living body by generating an electromagnetic antiseptic signal in proximity of the insertion site.

Provided herein are biomarkers that are useful in various aspects of treating and preventing complications and side effects associated with a disease or disorder, such as those associated with immune suppression, disruption of the gastrointestinal microbiome, infections (e.g., bloodstream infections), and / or GvHD, for example, in HSCT subjects. In some aspects, the biomarkers provided herein can be used in identifying a subject who is responding to a bacterial composition and / or subjects that are suitable for a treatment with a bacterial composition.

The present application relates to the field of medical detection, in particular to a fluorescence sensing composition, a fluorescence sensing film and application thereof. Through screening a variety of fluorescence indicators and combinations thereof, a simple and cost-effective indicator formula is finally obtained. Based on the formula, through optimizing the preparation process, a high-efficiency fluorescence sensing film resistant to moist heat sterilization is developed. After inoculating gram-negative bacilli, gram-positive cocci and yeast-type fungi representative quality control strains into the microorganism culture bottle prepared based on the film, through fluorescence curve determination of the full-automatic microorganism culture system, gram staining, microorganism mass spectrum identification and other experiments, it is proved that the fluorescence sensing film has excellent detection performance. The fluorescence sensing film proposed in the present application can be applied to the detection of clinical microorganisms, which can provide technical support for the diagnosis of bloodstream infections, and has important significance for curbing the abuse of antibiotics and improving the prognosis of patients with bloodstream infections.

The invention belongs to the technical field of bloodstream infection detection, and relates to a primer group for detecting bloodstream infection related pathogens, a kit and application. The invention provides a primer group for detecting blood flow infection related pathogens. The primer group comprises an upstream primer with a nucleotide sequence as shown in SEQ ID NO: 1-26 and a downstream primer with a nucleotide sequence as shown in SEQ ID NO: 27-52. The primer group provided by the invention can realize detection of 13 pathogens in blood flow infection related pathogens, and the pathogens cover common gram-negative bacteria, gram-positive bacteria and fungi and have good sensitivity and specificity.

The present application relates to blood culture bottle technical field, especially to composition and its application in the preparation of microbial culture medium, the present application combines yeast extract powder, soybean peptone and pancreatic casein peptone into nitrogen source, forming aerobic and anaerobic culture medium for clinical pathogenic microorganism culture, through comparison and verification, the aerobic and anaerobic culture medium added with the composition as described above is superior to prior art and other formula in culture speed and biomass accumulation in the growth experiment of 9 aerobic bacteria and 7 anaerobic bacteria, which shows that the composition as described above can effectively promote the performance of blood culture product, improve the ability of clinical blood stream infection detection, curb antibiotic abuse and save patients' lives.

Provided herein are biomarkers that are useful in various aspects of treating and preventing complications and side effects associated with a disease or disorder, such as those associated with immune suppression, disruption of the gastrointestinal microbiome, infections (e.g., bloodstream infections), and / or GvHD, for example, in HSCT subjects. In some aspects, the biomarkers provided herein can be used in identifying a subject who is responding to a bacterial composition and / or subjects that are suitable for a treatment with a bacterial composition.

The invention discloses a method for rapidly enriching and identifying blood flow infection pathogenic bacteria based on a nano material-liquid chromatography-mass spectrometry technology and a kit thereof, and the method comprises the following steps: (1) taking a blood culture positive culture solution, adding nano magnetic beads, blowing, beating, oscillating and uniformly mixing; (2) magnetically absorbing and abandoning the supernate, adding an enzyme digestion buffer solution into the sample, repeatedly cleaning, and abandoning the supernate; (3) adding the enzyme digestion buffer solution into the sample again, uniformly mixing, and adding the protease solution for enzyme digestion; (4) carrying out high-speed centrifugation or magnetic attraction, and carrying out mass spectrum identification on supernate; and (5) carrying out database searching qualitative analysis on the obtained mass spectrum data, and identifying to obtain the pathogenic bacteria variety. According to the invention, pathogens and magnetic beads are creatively formed into a complex, and proteomics analysis is carried out on the complex, so that the whole sample treatment time is greatly shortened, and precious time is provided for accurate medical treatment and medication of patients.

The present invention relates to peptides, fusion proteins and compositions for inducing an immune response against bacteria. The invention also relates to the treatment or prevention of infections by sepsis-inducing bacteria, and to the treatment and prevention of pathological conditions caused by such bacteria. Such pathological conditions include sepsis, pneumonia, meningitis, endocarditis, enterocolitis, urinary tract infection, soft tissue infection, gastrointestinal tract infection, bloodstream infection, encephalitis, premature delivery and dead delivery.